CN106266390B - A kind of sedge extract and its application - Google Patents
A kind of sedge extract and its application Download PDFInfo
- Publication number
- CN106266390B CN106266390B CN201610686419.6A CN201610686419A CN106266390B CN 106266390 B CN106266390 B CN 106266390B CN 201610686419 A CN201610686419 A CN 201610686419A CN 106266390 B CN106266390 B CN 106266390B
- Authority
- CN
- China
- Prior art keywords
- sedge
- extract
- water
- material liquid
- alcohol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241001290610 Abildgaardia Species 0.000 title claims abstract description 108
- 239000000284 extract Substances 0.000 title claims abstract description 99
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 60
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 238000009499 grossing Methods 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 52
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 39
- 239000007788 liquid Substances 0.000 claims description 36
- 235000019441 ethanol Nutrition 0.000 claims description 30
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 24
- 239000002195 soluble material Substances 0.000 claims description 22
- 239000000706 filtrate Substances 0.000 claims description 13
- 239000002904 solvent Substances 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 8
- QYYMDNHUJFIDDQ-UHFFFAOYSA-N 5-chloro-2-methyl-1,2-thiazol-3-one;2-methyl-1,2-thiazol-3-one Chemical compound CN1SC=CC1=O.CN1SC(Cl)=CC1=O QYYMDNHUJFIDDQ-UHFFFAOYSA-N 0.000 claims description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 6
- 229930003944 flavone Natural products 0.000 claims description 6
- 235000011949 flavones Nutrition 0.000 claims description 6
- 239000002304 perfume Substances 0.000 claims description 6
- 239000012286 potassium permanganate Substances 0.000 claims description 6
- 239000003755 preservative agent Substances 0.000 claims description 6
- 230000002335 preservative effect Effects 0.000 claims description 6
- 239000000654 additive Substances 0.000 claims description 5
- 230000000996 additive effect Effects 0.000 claims description 5
- 150000002213 flavones Chemical class 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 239000003945 anionic surfactant Substances 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 206010047601 Vitamin B1 deficiency Diseases 0.000 abstract description 6
- 208000002894 beriberi Diseases 0.000 abstract description 6
- 230000003078 antioxidant effect Effects 0.000 abstract description 4
- 239000003963 antioxidant agent Substances 0.000 abstract description 2
- 239000002537 cosmetic Substances 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000003115 biocidal effect Effects 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 24
- 239000000523 sample Substances 0.000 description 21
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 20
- -1 hydroxyl radical free radical Chemical class 0.000 description 17
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
- 239000012153 distilled water Substances 0.000 description 11
- 229960004756 ethanol Drugs 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 10
- 229930003268 Vitamin C Natural products 0.000 description 10
- 238000002835 absorbance Methods 0.000 description 10
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 10
- 235000019154 vitamin C Nutrition 0.000 description 10
- 239000011718 vitamin C Substances 0.000 description 10
- 238000005259 measurement Methods 0.000 description 9
- 239000000047 product Substances 0.000 description 8
- 230000002000 scavenging effect Effects 0.000 description 8
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 7
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
- 239000006286 aqueous extract Substances 0.000 description 5
- 230000003385 bacteriostatic effect Effects 0.000 description 5
- 229960000935 dehydrated alcohol Drugs 0.000 description 5
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 5
- 229960004889 salicylic acid Drugs 0.000 description 5
- 239000012488 sample solution Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 241000194103 Bacillus pumilus Species 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- 241000222122 Candida albicans Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 230000003213 activating effect Effects 0.000 description 4
- 229940095731 candida albicans Drugs 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 3
- 239000012490 blank solution Substances 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 230000031700 light absorption Effects 0.000 description 3
- 239000012263 liquid product Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 229940079877 pyrogallol Drugs 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- 125000000129 anionic group Chemical group 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000006701 autoxidation reaction Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 238000009631 Broth culture Methods 0.000 description 1
- 241000722731 Carex Species 0.000 description 1
- 241000045459 Carex meyeriana Species 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 241000130764 Tinea Species 0.000 description 1
- 208000002474 Tinea Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 210000003056 antler Anatomy 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229960005150 glycerol Drugs 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 229960002163 hydrogen peroxide Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical class [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 235000019394 potassium persulphate Nutrition 0.000 description 1
- LJCNRYVRMXRIQR-OLXYHTOASA-L potassium sodium L-tartrate Chemical compound [Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O LJCNRYVRMXRIQR-OLXYHTOASA-L 0.000 description 1
- 229940074439 potassium sodium tartrate Drugs 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 238000009991 scouring Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 235000011006 sodium potassium tartrate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/89—Cyperaceae (Sedge family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Dermatology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Birds (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The present invention is a kind of sedge extract and its application, which is sedge water extract.The present invention prepares the smoothing toner, beriberi liquor and resurgent water to work well using the sedge extract, the extract has good inoxidizability and biocidal property, can be used as preparation of the raw material applied to antioxidant health-care product, food, cosmetics, cleaning supplies and drug.
Description
Technical field
The present invention relates to natural extracts preparation field, specially a kind of sedge extract and its application.
Background technique
Sedge Carex meyeriana Kunth is that Cyperaceae sedge belongs to Carex L. herbaceos perennial.1837
Year is first recorded in Polish " Enum II "." Chinese Plants will " record of volume 12, sedge integrated distribution are remote in China northeast, Russia
The states such as eastern area and Siberia, Mongolia, Korea.The applicating history of sedge is long, as the record of inner sock object, has
The history of centuries.Because it has the characteristics that peculiar heat-preserving function and ventilative, absorbing sweat, moisture-proof, cold-proof, antibacterial, main product in addition
In the Northeast, China, and ginseng, pilose antler and claim " northeast Triratna ".In view of the long Popular Utilization history of sedge and unique
Effect in addition to insole, develops sedge serial health product, such as back belt, mattress, pillow with neck, cushion, automobile in recent years
Cushion etc..Then, so far, the research of sedge active constituent is but rarely reported, causes the exploitation of this precious resources
Still in the low-level repetition stage.
Summary of the invention
The purpose of the present invention is to provide a kind of sedge extract and its applications, extract the active constituent of sedge,
Improve the comprehensive utilization of sedge resource.The purpose of the present invention is what is be achieved through the following technical solutions.
A kind of sedge extract, the extract is using sedge as raw material, using water as Extraction solvent.
Further, the preparation step of the extract are as follows: by sedge grinding and sieving, the sedge after sieving is added
Solvent extracts 8~10 hours, and solid-liquid ratio is 1:1~1:20, natural filtration, filter residue repetition extraction, merging filtrate, decompression steaming
Except solvent obtains sedge extract.
Further, the preparation step of the extract are as follows: sedge is crushed to 0.45~0.55cm, solvent is added,
Solid-liquid ratio is 1:10~1:20, and in 85~95 DEG C of 2~3h of extraction, filtering, filter residue repeats to extract 1~3 time, merges to obtain total filtrate,
And it is evaporated under reduced pressure to the 1/2 of total filtrate volume and obtains sedge extract.
A kind of sedge smoothing toner, the smoothing toner include the sedge extract and glycerol, propylene glycol and ethyl alcohol.
The preparation method of the smoothing toner,
Including following preparation step:
1) the sedge extract is added to the water, sequentially adds glycerol, propylene glycol, soluble perfume, stirred molten
Solution, obtains water-soluble material liquid, and the sedge extract, glycerol, propylene glycol account for the volume fraction point of the water-soluble material liquid
Not Wei 50%~65%, 15.0%~20.0%, 1.5%~5.0%, the additive amount of soluble perfume is 0.05~0.2g/
100mLWater-soluble material liquid；
2) preservative is dissolved in the ethanol solution that volume fraction is 10.0%~20.0%, stirring and dissolving obtains alcohol-soluble
Material liquid, the preservative are the mixture of any one in Kathon CG and EDTA or both, the addition of the Kathon CG and EDTA
Amount is respectively 0.05~0.10g/100mLAlcohol-soluble material liquidWith 0.10~0.15g/100mLAlcohol-soluble material liquid；
3) the water-soluble material liquid and the alcohol-soluble material liquid are mixed, is stirred evenly, left undisturbed overnight, filtered, dress
Bottle.
A kind of sedge beriberi liquor includes the sedge extract and salicylic acid, ethyl alcohol, dioxygen in the beriberi liquor
Water, glycerol and acetic acid, the mass fraction of each ingredient are as follows: sedge extract 90~95, salicylic acid 0.5~2 are ethyl alcohol 4~6, double
Oxygen water 0.5~2, glycerol 1~3, acetic acid 0.5~2.
A kind of sedge resurgent water, the resurgent water include the sedge extract, potassium permanganate, anionic surface
Activating agent and water, the mass fraction of each ingredient are as follows: sedge extract 70~80, potassium permanganate 0.001~0.01, anion table
Face activating agent 5~20, water 5~25.
Beneficial effects of the present invention:
1) present invention is extracted the good sedge extract of antioxidant activity from " northeast Triratna " sedge, which draws
Careless extract can be applied to health care product, cosmetics and field of medicaments, for this characteristic resources deeply develop provide it is new
Development approach；
2) preparation method of sedge extract provided by the present invention is simple, strong operability, environmental-friendly, industrialization
It is larger to amplify potentiality；
3) this it is clearly demarcated in sedge extract also there is good bacteriostatic activity while there is antioxidant activity, can open
Issue the daily chemical products such as smoothing toner, beriberi liquor.
Detailed description of the invention
Fig. 1 is Scavenging activity figure of the sedge extract to hydroxyl radical free radical.
Fig. 2 is Scavenging activity figure of the sedge extract to DPPH free radical.
Fig. 3 is Scavenging activity figure of the sedge extract to ABTS free radical.
Fig. 4 is Scavenging activity figure of the sedge extract to superoxide anion.
Specific embodiment
Embodiment 1
A kind of sedge extract, the extract the preparation method comprises the following steps: by sedge be crushed to length be 0.5cm ±
0.05cm, smashed sedge are sieved in 60 mesh, are put in a round bottom flask, and solvent is added and extracts 8~10h, in normal pressure
Under be filtered, be concentrated to the 1/2 of stoste with Rotary Evaporators, filter residue repeats to extract twice, merging filtrate, evaporating solvent under reduced pressure
Obtain sedge extract.
Extraction solvent is water, and solid-liquid ratio is 1:1~1:20, preferably 1:10.
Embodiment 2
A kind of sedge extract, the preparation step of the extract are as follows: sedge is crushed to 0.45~0.55cm, is added
Enter water, solid-liquid ratio is 1:10~1:20, and in 85~95 DEG C of 2~3h of extraction, filtering, filter residue repeats to extract 1~3 time, merges always
Filtrate, and be evaporated under reduced pressure to the 1/2 of total filtrate volume and obtain sedge extract.
Embodiment 3
A kind of sedge smoothing toner,
Sedge extract is prepared in preparation process first:
Sedge is crushed to 0.45~0.55cm, water is added, solid-liquid ratio is 1:10~1:20 (preferably 1:10), 85~
95 DEG C (preferably 90 DEG C) 2~3h of extraction, filtering, filter residue repeat to extract 1~3 time, merge to obtain total filtrate, and are evaporated under reduced pressure to total filter
The 1/2 of liquid product obtains sedge extract.
Subsequent preparation step:
1) the sedge extract is added to the water, sequentially adds glycerol, propylene glycol, soluble perfume, stirred molten
Solution, obtains water-soluble material liquid, and the sedge extract, glycerol, propylene glycol account for the volume fraction point of the water-soluble material liquid
Not Wei 50%~65%, 15.0%~20.0%, 1.5%~5.0%, the additive amount of soluble perfume is 0.05~0.2g/
100mLWater-soluble material liquid；
2) preservative is dissolved in the ethanol solution that volume fraction is 10.0%~20.0%, stirring and dissolving obtains alcohol-soluble
Material liquid, the preservative are the mixture of any one in Kathon CG and EDTA or both, the addition of the Kathon CG and EDTA
Amount is respectively 0.05~0.10g/100mLAlcohol-soluble material liquidWith 0.10~0.15g/100mLAlcohol-soluble material liquid；
3) the water-soluble material liquid and the alcohol-soluble material liquid are mixed, is stirred evenly, left undisturbed overnight, filtered, dress
Bottle.
Since sedge aqueous extract has anti-oxidant and bacteriostasis, this product can reach the effect of skin nutrition, prolong
The aging of slow skin, product are easy to use.
Embodiment 4
A kind of sedge beriberi liquor,
Sedge extract is prepared in preparation process first:
Sedge is crushed to 0.45~0.55cm, water is added, solid-liquid ratio is 1:10~1:20 (preferably 1:10), 85~
95 DEG C (preferably 90 DEG C) 2~3h of extraction, filtering, filter residue repeat to extract 1~3 time, merge to obtain total filtrate, and are evaporated under reduced pressure to total filter
The 1/2 of liquid product obtains sedge extract.
Comprising the sedge extract and salicylic acid, ethyl alcohol, hydrogen peroxide, glycerol and acetic acid in the beriberi liquor, respectively
The mass fraction of ingredient are as follows: sedge extract 90~95, salicylic acid 0.5~2, ethyl alcohol 4~6, hydrogen peroxide 0.5~2, glycerol 1
~3, acetic acid 0.5~2, preferably sedge extract 90%, salicylic acid 1%, ethyl alcohol 5%, hydrogen peroxide 1%, 2% glycerol, 1% vinegar
Water-soluble, the faint yellow aqueous solution for having sedge smell of pale green-is made in acid.The aqueous solution has stronger sterilization, antibacterial function
Energy.The sweat gland secretion of adjustable human body has good effect to foot odour, treatment tinea of feet and hands is removed.The product can be made into wiping
Agent or lotion are directly administered, and can also be sprayed on socks, insole indirect delivery.
Embodiment 5
A kind of sedge resurgent water,
Sedge extract is prepared in preparation process first:
Sedge is crushed to 0.45~0.55cm, water is added, solid-liquid ratio is 1:10~1:20 (preferably 1:10), 85~
95 DEG C (preferably 90 DEG C) 2~3h of extraction, filtering, filter residue repeat to extract 1~3 time, merge to obtain total filtrate, and are evaporated under reduced pressure to total filter
The 1/2 of liquid product obtains sedge extract.
A kind of sedge resurgent water, the resurgent water include the sedge extract, potassium permanganate, anionic surface
Activating agent and water, the mass fraction of each ingredient are as follows: sedge extract 70~80, potassium permanganate 0.001~0.01, anion table
Face activating agent 5~20, water 5~25.
Water-soluble, the faint yellow aqueous solution for having sedge smell is made in mentioned component.It is dipped at quarterly intervals with dry scouring pad
Resurgent water is wiped repeatedly wall painting surface, can remove dust and dirt, and can keep the distinctive side's fragrance breath of sedge.
The antioxidation activity in vitro of sedge extract provided by the present invention is evaluated and experimental result:
1. the measurement of sedge extract removing hydroxyl radical free radical ability
It with reference to Fenton method, takes 0.75mmol/L Phen 1mL in test tube, sequentially adds PBS (pH 7.4) 2mL,
Distilled water 1mL after mixing well, is added 0.75mmol/L ferrous sulfate 1mL, mixes, and adding mass fraction is 0.12% H2O2
1mL, 37 DEG C of water-bath 60min measure its absorbance A at 536nmpValue；H is replaced with distilled water2O2, measure its absorbance AbValue；
It takes and replaces distilled water 1mL with ethyl alcohol dilution 1,0.2,0.1,0.07, the sedge alcohol extract solution of 0.05mg/mL respectively, measurement
Its absorbance AsValue.Separately take the sedge water extract (crow i.e. in embodiment 2 of 0.07,0.05,0.04,0.03,0.02mg/mL
Careless extract being drawn, similarly hereinafter) solution replaces distilled water 1mL, measure another set absorbance AsValue.0.1,0.2,0.5,0.7,1mg/
ML vitamin C (VC) is positive control.Sample is calculated to the clearance rate of hydroxyl radical free radical, formula is as follows:
Clearance rate (%)=(As-Ap)/(Ab-Ap) × 100%
As shown in Figure 1, within the scope of 0.02~1mg/mL mass concentration, sedge alcohol extract (is replaced with same volume ethyl alcohol
Water extracts to obtain sedge alcohol extract, similarly hereinafter) and water extract be in dose-effect relationship to the Scavenging activity of hydroxyl radical free radical, with mass concentration
Increase, clearance rate increases.Wherein, sedge water extract removing hydroxyl radical free radical ability is better than alcohol extract: when water extract concentration reaches
When to 0.07mg/mL, clearance rate is maximum, is 91.85 ℅；And when alcohol extract concentration reaches 1mg/mL, maximal clearance only has
84.12℅.The two is superior to VC to the Scavenging activity of hydroxyl radical free radical.
2. the measurement of sedge extract scavenging ability of DPPH free radical
The sedge alcohol extract solution 2mL of 2,1,0.5,0.33,0.25mg/mL is taken, isometric concentration, which is added, is
The DPPH solution (being prepared with dehydrated alcohol) of 0.1mmol/L, is protected from light 30min at room temperature, measures at 517nm after mixing
Absorbance Ai；And it returns to zero using 4mL dehydrated alcohol and distilled water (the two is respectively 2mL) as blank.Blank control group is 2mL sample
Product liquid adds 2mL dehydrated alcohol, and the light absorption value at 517nm is Aj, be arranged blank control group the purpose of be for Polysaccharide removing
The light absorption value of sample itself.Model control group is that 2mL DPPH solution adds 2mL dehydrated alcohol, the light absorption value at 517nm
For A0.Changing mass concentration into respectively is 2, the sedge water extract solution of 1,0.2,0.1,0.067mg/mL, 0.0033,
0.0065, the VC of 0.0125,0.025,0.05mg/mL, repeats the above process.Sample is calculated to the clearance rate of DPPH free radical,
Formula is as follows:
Clearance rate (%)=[1- (Ai-Aj)/Ao] × 100%
As shown in Figure 2, within the scope of 0.067~2mg/mL mass concentration, sedge alcohol extract and water extract to DPPH from
Dose-effect relationship is presented by the Scavenging activity of base, is increased with mass concentration, power enhancing is removed.When mass concentration reaches 2mg/mL, water
The clearance rate of extract is 99.7 ℅, and alcohol extract is 62.8 ℅, shows that the ability of sedge water extract removing DPPH free radical is better than
Alcohol extract, but the two is weaker than VC.
3. the measurement of sedge extract removing ABTS free radical ability
5mL 7mmoL/L ABTS and 88 μ L 140mmoL/L potassium peroxydisulfates are mixed, stood under the conditions of room temperature is protected from light
Night (12-16h) forms ABTS free radical stock solution, is then diluted to ABTS working solution with 1:50 ratio with distilled water, makes 30
DEG C, absorbance is 0.702 at 734nm wavelength.
Precision pipettes the sedge alcohol extracting of different quality concentration (2,0.33,0.2,0.1,0.067,0.05mg/mL) respectively
Object and each 2.0mL of water extract solution example are separately added into ABTS measurement liquid 2.0mL, accurately shake 10s, stand in test tube
After 6min, A is measured at 734nm.Dehydrated alcohol 2.0mL is pipetted in test tube simultaneously, is handled in the same way, is measured Ao。
Using 1,2,2.5,3mg/mL VC solution as positive control.Replication 3 times, calculate clearance rate.
Clearance rate (%)=(Ao-ASample)/Ao× 100%
From the figure 3, it may be seen that within the scope of 0.05~2mg/mL mass concentration, removing of the sedge alcohol extract to ABTS free radical
Power first increases and then decreases, when concentration reaches 0.33mg/mL, clearance rate is maximum, is 79.36 ℅；Water extract clearance rate is but with dense
Degree increases and reduces, and when concentration is 0.05mg/mL, clearance rate is maximum, is 80.83 ℅.It is comprehensively compared, sedge water extract pair
The removing power of ABTS free radical is greater than alcohol extract.Meanwhile the two removes power and is better than VC, when concentration reaches 3mg/mL, VC's
Clearance rate is 60.21 ℅.
4. the measurement of sedge extract removing ultra-oxygen anion free radical ability
50mmol/L Tris-HCl buffer (pH 8.2, EDTA-2Na containing 2mmol/L) 4.5mL is taken, is separately added into not
Sedge alcohol extract, water extract and each 1mL of VC solution of homogenous quantities concentration (0.01,0.02,0.03,0.05,0.07mg/mL),
Pyrogallol (with the preparation of 10mmol/L hydrochloric acid) 0.2mL, pours into cuvette, immediately with isometric 10mmol/L hydrochloric acid generation after mixing
It is blank zeroing for pyrogallol solution, measures an absorbance A value every 30s at 325 nm wavelength, calculates in the range of linearity
A value changes with time value, and control group replaces sample solution with isometric deionized water.It calculates to ultra-oxygen anion free radical
Clearance rate.
Clearance rate (%)=(V1-V2)/V1× 100%
In formula, V1For the autoxidation rate of control tube pyrogallol；V2For the autoxidation rate of sample cell pyrogallol.
As shown in Figure 4, when mass concentration is 0.01~0.07mg/mL, sedge alcohol extract and water extract are to super oxygen yin
The removing power of ion is in dose-effect relationship.Alcohol extract removes power and increases with concentration and enhance, and it is 63.79 ℅ that maximum, which removes power,；Water mentions
Object is removed power and is then increased with concentration, and presentation first enhances, the trend weakened afterwards, and maximal clearance is 78.16 ℅, corresponding mass concentration
For 0.05mg/mL.The above result shows that sedge water extract is better than alcohol extract to the removing power of superoxide anion, it is suitable with VC
(maximal clearance is 78.74 ℅).
The identification of aqueous extract chemical component and active constituent content measuring:
Sedge crushes about 0.5cm or so, is put into round-bottomed flask and extracts, and is mentioned with water (1:10, w:v) as 90 DEG C of solvent
Take 2~3h, extract 2 times, merging filtrate, natural filtration, filtrate is concentrated to the 1/2 of stoste volume, carry out chemical component identification and
Assay.
One, chemical component is identified
It takes the above-mentioned sample of 2mL to be separately added into 10mL test tube, carries out chemical component identification.
1. protein
(1) sample heating is boiled, is precipitated；
(2) 5%H is added in sample2SO4, do not heat and precipitate；
(3) add 40%NaOH solution and 1%CuSO in sample4There is brownish red in solution；
(4) add 0.2% ninhydrin solution in sample, bluish violet occur.
Contain protein in above four description of test samples.
2. sugar
3 test tubes are taken to carry out following 3 experiments:
(1) add CuSO in sample4, potassium sodium tartrate solution there is brick-red precipitating；
(2) add the dense H of alpha-Naphthol-in sample2SO4There is purplish red colour circle；
(3) add 0.1mol/LAgNO in sample3, 5mol/L ammonium hydroxide there is silver-colored brown.
The above experimental result illustrates in sample containing sugar.
3. flavones
(1) a small amount of magnesium powder (or zinc powder) is added in sample, then enriching HCl 4~5 drips, 2~3min of boiling water bath, occurs red
Color.
(2) alchlor reacts: sample spot after doing, is sprayed alchlor ethanol solution, sees under ultraviolet lamp on filter paper
It examines, yellow is presented.
The above experimental result illustrates to contain flavone compound in sample.
Two, assay
1. the measurement of protein content
Precision pipettes sample solution 1.0mL, and distilled water 1.0mL is added, and it is molten that 5.0mL Coomassie brilliant G-250 is then added
Liquid shakes up colour developing, as solution to be measured；Precision pipettes distilled water 2.0mL, is placed in test tube, and Coomassie brilliant G-250 examination is added
Agent 5.0mL shakes up colour developing, as blank solution.After solution to be measured and the static 2min of blank solution, surveyed at 595nm wavelength
Determine absorbance value, Bovine serum albumin standard curve is y=5.4954x-0.2806.Three groups of parallel laboratory tests are done, protein is measured
Content is 0.338%.
2. measurement of the polysaccharide content
Sample solution is diluted 30 times, 0.5mL dilution is pipetted in test tube, adds the phenol solution and 3.5mL of 1mL 5%
Dense H2SO4, oscillation mixes, and by mixed liquor in 40 DEG C of water bath processing 30min, cools down 15min at room temperature, measures it at 490nm
Absorbance value.Blank solution is done with distilled water instead of sample diluting liquid, glucose standard curve y=6.6671x+0.0202,
Measuring polyoses content is 2.26%.
3. the measurement of flavones content
Sample solution is diluted 10 times, 2mL dilution is pipetted in volumetric flask, 1% alchlor 4mL is added, is added
60% ethyl alcohol is settled to 10mL.15min is stood at room temperature, and absorbance value is measured at 400nm wavelength.It brings standard curve into and asks yellow
Ketone concentration, blank control group are that flavones is not added, and rutin standard curve y=23.994x+0.0046, measuring flavones content is
0.195%.
The test of sedge Aqueous extracts bacteriostatic activity:
Select Escherichia coli, bacillus subtilis, bacillus pumilus, staphylococcus aureus, Candida albicans for inspection
Determine bacterium, investigates the bacteriostatic activity of sedge aqueous extract.
One, culture medium
Escherichia coli, bacillus subtilis, bacillus pumilus, staphylococcus aureus: trypticase soybean broth culture
Base；
Candida albicans: Sabouraud dextrose medium.
Two, different strains condition of culture
Escherichia coli, bacillus subtilis, staphylococcus aureus, bacillus pumilus: 35 DEG C of constant temperature, culture is for 24 hours；
Candida albicans: 25 DEG C of constant temperature, 48h is cultivated.
Three, bacteriostatic test
1. the preparation of bacteria suspension
Strain is inoculated in corresponding slant medium, is cultivated by corresponding condition of culture；It will with 10mL sterile water
Bacterium on inclined-plane, which is washed down, to pour into triangular flask, is counted with blood counting chamber, and bacteria concentration is adjusted 108Cfu/mL~109cfu/mL
In range, obtain bacteria suspension and be put into 4 DEG C of refrigerator to save backup.
2. the production of the sample scraps of paper
200mL sedge aqueous extract is concentrated, solid is dried to obtain.Being each configured to concentration by solvent of distilled water is
The sample solution of 1mg/mL and 5mg/mL.
Filter paper is broken into the circular paper that diameter is 6mm with punch, and the 20 above-mentioned solution of μ L are added dropwise, each concentration includes
4 scraps of paper, each 5 groups, each group spontaneously dries spare using distilled water as blank control.
3. the operation of paper disk method experiment
Different culture medium is poured into and is prepared into plate in plate, 100 μ L bacteria suspensions is taken to be spread evenly across culture base table respectively
Face.The sample scraps of paper are put on corresponding plate with tweezers, plate is inverted and is cultivated.It is Escherichia coli, bacillus subtilis, golden yellow
Color staphylococcus, bacillus pumilus are put into 35 DEG C of constant incubator and cultivate for 24 hours, and Candida albicans puts 25 DEG C of constant temperature into
48h is cultivated in incubator, observes inhibition zone.
4. result
For the blank control scraps of paper without inhibition zone, all samples scraps of paper have inhibition zone, illustrate 1mg/mL~5mg/mL concentration
Sedge water extract in range has bacteriostatic activity to 5 kinds of test organisms.
Claims (3)
1. a kind of sedge extract, it is characterised in that: the extract is using sedge as raw material, using water as Extraction solvent；
Sedge is crushed to 0.5cm, solvent is added, solid-liquid ratio 1:10, in 90 DEG C of 2 ~ 3h of extraction, filtering, filter residue repeats to mention
It takes 2 times, merges to obtain total filtrate, and be evaporated under reduced pressure to the 1/2 of total filtrate volume and obtain sedge extract, wherein protein content is
0.338%, polyoses content 2.26%, flavones content 0.195%.
2. a kind of sedge smoothing toner, it is characterised in that: the smoothing toner include sedge extract described in claim 1 and
Glycerol, propylene glycol and ethyl alcohol；Including following preparation step:
The sedge extract is added to the water, glycerol, propylene glycol, soluble perfume are sequentially added, stirring and dissolving obtains water
Dissolubility material liquid, the sedge extract, glycerol, propylene glycol account for the volume fraction of the water-soluble material liquid be respectively 50% ~
65%, 15.0% ~ 20.0%, 1.5% ~ 5.0%, the additive amount of soluble perfume be every 100mL water-soluble material liquid addition 0.05 ~
0.2g；
Preservative is dissolved in the ethanol solution that volume fraction is 10.0% ~ 20.0%, stirring and dissolving obtains alcohol-soluble material liquid, institute
The mixture that preservative is any one in Kathon CG and EDTA or both is stated, the additive amount of the Kathon CG is that every 100mL alcohol is molten
Property material liquid add 0.05 ~ 0.10g, the additive amount of EDTA is respectively that every 100mL alcohol-soluble material liquid adds 0.10 ~ 0.15g；
The water-soluble material liquid and the alcohol-soluble material liquid are mixed, stirred evenly, left undisturbed overnight, is filtered, bottling.
3. a kind of sedge resurgent water, it is characterised in that: the resurgent water include sedge extract described in claim 1,
Potassium permanganate, anionic surfactant and water, the mass fraction of each ingredient are as follows: sedge extract 70 ~ 80, potassium permanganate
0.001 ~ 0.01, anionic surfactant 5 ~ 20, water 5 ~ 25.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610320619X | 2016-05-16 | ||
| CN201610320619 | 2016-05-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106266390A CN106266390A (en) | 2017-01-04 |
| CN106266390B true CN106266390B (en) | 2019-10-11 |
Family
ID=57679955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610686419.6A Active CN106266390B (en) | 2016-05-16 | 2016-08-18 | A kind of sedge extract and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106266390B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108392449A (en) * | 2018-05-24 | 2018-08-14 | 广东山石微量元素研究院（普通合伙） | A kind of Khotan jade anti-aging lotion and preparation method thereof |
| CN111851145A (en) * | 2020-06-28 | 2020-10-30 | 杭州壹澍美生态科技发展有限公司 | Antibacterial and mothproof Wula sedge wallpaper and processing technology thereof |
| CN111973529A (en) * | 2020-09-04 | 2020-11-24 | 吉林化工学院 | Moisturizing whitening cream and preparation method thereof |
| CN112438400A (en) * | 2020-11-13 | 2021-03-05 | 吉林化工学院 | Method for purifying Meyer sedge total flavonoids and application thereof |
| CN112516232A (en) * | 2020-12-25 | 2021-03-19 | 长春中医药大学 | Antibacterial, anti-inflammatory and antioxidant carex meyeriana extract, and preparation method and application thereof |
| CN113712883B (en) * | 2021-09-28 | 2022-11-15 | 植物医生(广东)生物科技有限公司 | Repairing cream containing dendrobium officinale extract and preparation method of repairing cream |
| CN114404508A (en) * | 2022-02-23 | 2022-04-29 | 吉林农业科技学院 | Preparation method of Meyer sedge extract |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367235A (en) * | 2001-12-03 | 2002-09-04 | 阎淑梅 | Black ant liquor and its preparation method |
-
2016
- 2016-08-18 CN CN201610686419.6A patent/CN106266390B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1367235A (en) * | 2001-12-03 | 2002-09-04 | 阎淑梅 | Black ant liquor and its preparation method |
Non-Patent Citations (1)
| Title |
|---|
| "乌拉草化学成分和药理作用的初步研究";余克娇;《中国优秀博硕士学位论文全文数据库（硕士） 医药卫生科技辑》;20070115(第1期);正文第8页第2、4、6段、第9页第1段、第10页第3-5段、第15页第2段和最后一段、第16页第1、3段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106266390A (en) | 2017-01-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106266390B (en) | A kind of sedge extract and its application | |
| CN106726898A (en) | A kind of Ramulus et Folium Mussaendae Pubescentis extract and preparation method and application | |
| CN102940597B (en) | Natural compound botanical antioxidant, as well as preparation method and application thereof in cosmetics | |
| CN103462858B (en) | There is the Herba bromi japonici mud, facial film and preparation method thereof of clean skin-care effect | |
| Macwan et al. | A comparative evaluation of in vitro antioxidant properties of Bamboo Bambusa arundinacea leaves extracts | |
| CN105596243A (en) | Essence liquid containing nano-gold and thermal spring water and mask | |
| CN108714126A (en) | Skin care compositions and the preparation method and application thereof with moisturizing and anti-oxidation efficacy | |
| CN109528843A (en) | A kind of extracting method of flavone of hawthorn fruit | |
| KR102626140B1 (en) | Anti-pollution cosmetic composition containing camellia extract, aurea helianthus extract, carpinus tschonoskii extract, silkworm extract and centella asiatica extract | |
| JP5600239B2 (en) | Method for producing lignin extract and lignin extract | |
| KR101839491B1 (en) | Extraction Method of Houttuynia cordata Thunberg Extract and Cosmetic materials Using the same | |
| Malam et al. | Comparative in vitro antioxidant studies of ethanolic extracts of Psidium guajava stem bark and Telfairia occidentalis leaf | |
| CN105832648B (en) | A kind of nourishing anti-aging peony seed oil mask sheet and preparation method thereof | |
| CN108938479A (en) | A kind of mildy wash of the extract containing water lily and preparation method thereof | |
| Rahim et al. | Antioxidant activity of alstonia Angustifolia ethanolic leaf extract | |
| CN108653037A (en) | Skin conditioning agent composition, its application and whitening mask | |
| KR102251189B1 (en) | Manufacturing method of polysaccharide and cosmetic composition | |
| CN106924117A (en) | The washing product composition prepared with remaining artemisia annua residue after extraction qinghaosu | |
| KR101435872B1 (en) | Cosmetic composition for anti-oxidation and anti-aging comprising Ginseng seeds extract | |
| CN105342917A (en) | Whitening cream based on natural extracts, and preparation method thereof | |
| CN110448602A (en) | A kind of extracting method and its application of tree peony proanthocyanidin | |
| CN110179734A (en) | A kind of reparation moisture retention liquid and facial mask liquid and preparation method thereof | |
| CN110141543A (en) | Has the orchid extract and combinations thereof of anti-human yeast-like fungi function | |
| TW201904599A (en) | Dragon fruit flower extract, preparation method thereof, use thereof and composition for improving skin condition | |
| KR101608882B1 (en) | Cosmetic Composition Comprising Golden Peanut Extracts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Room 7088-1330, 7th Floor, No. 1558 Jiangnan Road, Ningbo High tech Zone, Zhejiang Province Patentee after: Ningbo Yili Ula Grass Products Co.,Ltd. Address before: 132001 Tian Tai Yi She, Dongcheng Street, Longtan District, Jilin City, Jilin Province Patentee before: JILIN YILI ULA GRASS PRODUCTS CO.,LTD. |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20240613 Address after: Room 2702-2, No. 1357 Yinxian Avenue, Shounan Street, Yinzhou District, Ningbo City, Zhejiang Province, 315000 Patentee after: Ningbo Duoli Ula Grass Products Co.,Ltd. Country or region after: China Address before: Room 7088-1330, 7th Floor, No. 1558 Jiangnan Road, Ningbo High tech Zone, Zhejiang Province Patentee before: Ningbo Yili Ula Grass Products Co.,Ltd. Country or region before: China |