CN106260792A - A kind of micro-ecological feed preventing grass carp parent fishes enteritis and using method thereof - Google Patents
A kind of micro-ecological feed preventing grass carp parent fishes enteritis and using method thereof Download PDFInfo
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- CN106260792A CN106260792A CN201610923390.9A CN201610923390A CN106260792A CN 106260792 A CN106260792 A CN 106260792A CN 201610923390 A CN201610923390 A CN 201610923390A CN 106260792 A CN106260792 A CN 106260792A
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- Prior art keywords
- enteritis
- grass carp
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- ecological
- parent fishes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/132—Amines having two or more amino groups, e.g. spermidine, putrescine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/884—Alismataceae (Water-plantain family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention belongs to technical field of aquaculture, it is specifically related to a kind of its using method of micro-ecological feed preventing grass carp parent fishes enteritis, at expanded pellet diet surface spraying microbial ecological agent and prevention enteritis preparation, when grass carp parent fishes cultivation stage and the discovery ill sign of enteritis, described micro-ecological feed is used to substitute conventional feed.The present invention has the advantage that in the present invention that micro-ecological feed can antagonism pathogen compared to existing technology, improve animal body immunity, make full use of the mutuality of interest between strain, obtain strong stress resistance, the effectively microecological balance of environment in maintenance animal intestinal, microbial ecological agent and prevention enteritis preparation are sprayed on expanded granular feed surface, grass carp intestinal can be acted on fast and effectively, microbial ecological agent and prevention enteritis preparation synergism simultaneously, the effect improving regulation can be played for the intestinal condition of Ctenopharyngodon idellus, prevention and the effect for the treatment of can be effectively played, greatly reduce enteritis prevalence and the mortality rate of Ctenopharyngodon idellus.
Description
Technical field
The invention belongs to technical field of aquaculture, be specifically related to a kind of micro-ecological feed preventing grass carp parent fishes enteritis its
Using method.
Background technology
Ctenopharyngodon idellus is popular freshwater fish products, is the cheap animal protein sources of high-quality important in our people's day meals, the whole nation
Annual yield, at more than 400 ten thousand tons, is one of important cultured freshwater fish of China, the main phytophage fish raised as China
Veriety, the feeding habits of Ctenopharyngodon idellus begin to when the long 2.6cm of body convert to phytophage, and pasture and water, herbage are the Natural Foods of Ctenopharyngodon idellus eating
Thing, is also that the main bait source of conventional husbandry Ctenopharyngodon idellus is seen, along with the large-scale development in market, Ctenopharyngodon idellus nursery breeding is delayed, its
In main cause be that grass carp parent fishes enteritis prevalence is high, the most ill will be dead or affect growth performance, present
Conventional counte-rplan are to find ill rear treatment in time, but Ctenopharyngodon idellus nursery breeding can be made delayed seriously, affect culture benefit.
Summary of the invention
It is an object of the invention to for existing problem, it is provided that a kind of micro-ecological feed preventing grass carp parent fishes enteritis its
Using method.
The present invention is achieved by the following technical solutions: a kind of micro-ecological feed preventing grass carp parent fishes enteritis, swollen
Change feed surface spraying microbial ecological agent and prevention enteritis preparation；
Wherein, microbial ecological agent comprises: streptococcus faecalis viable count is 1 × 109-2×109Individual/ml, Deshi Lactobacillus viable count is
5×108-1×109Individual/ml, live lactobacillus acidophilus number is 3 × 109-4×109Individual/ml, Bacillus licheniformis viable count is 1
×109-4×109Individual/ml, Saccharomyces paradoxus bacterium viable count is 1 × 109-4×109Individual/ml；
Wherein, prevention enteritis preparation is mixed by the raw material of following weight portion: sulfoethyl zymosan 5, and cryptoxanthin 1 is sub-
Spermine 1, Rhizoma Alismatis extract 3, Freund adjuvant 20；
Wherein, in sulfoethyl zymosan, the substitution value of sulfoethyl is 0.736-0.852.
As further improvement of these options, described sulfoethyl zymosan is under alkaline system, by 2-chloroethyl
Sodium sulfonate and zymosan react preparation；Preparation condition is: zymosan is 2:5 with the weight ratio of 2-chloroethyl sodium sulfonate, hydrogen
The percentage by weight of sodium hydroxide solution is 35%, and during heating, microwave frequency is 280W, and the microwave time is 36 minutes.
As further improvement of these options, the preparation method of described microbial ecological agent comprises the following steps:
(1) preparing the mixed-culture medium of streptococcus faecalis, Deshi Lactobacillus and bacillus acidophilus, its inoculum concentration is culture volume
1%, after twice cultivation and get final product；The culture medium used contains the composition of following percentage by weight: glucose 2.2-2.5%,
Semen Maydis pulp 1.5-1.8%, Radix Raphani lyophilized powder 0.4-0.8%, bean milk 1.5-2.2%, carbamide 0.2-0.6%, hemoglobin 0.7-1.2%,
Calcium chloride 0.06-0.08%, potassium dihydrogen phosphate 0.02-0.04%, ammonium sulfate 0.05-0.08%, magnesium sulfate 0.01-0.02%, surplus
For water, pH value is 6.8-7.2；
(2) preparing Bacillus licheniformis and the mixed-culture medium of Saccharomyces paradoxus bacterium, its inoculum concentration is the 0.8% of culture volume, warp
After crossing twice cultivation and get final product；The culture medium used contains the composition of following percentage by weight: glucose 1.6-1.8%, Semen Maydis pulp
1.2-1.5%, Radix Raphani lyophilized powder 0.8-1.2%, bean milk 1.5-2.2%, carbamide 0.1-0.3%, hemoglobin 1.3-1.5%, calcium chloride
0.02-0.04%, potassium dihydrogen phosphate 0.05-0.08%, ammonium sulfate 0.05-0.08%, magnesium sulfate 0.01-0.02%, surplus are water, pH
Value is 7.2-7.4；
(3) it is 6 × 10 by viable count9-1×1010Streptococcus faecalis, viable count be 3 × 109-6×109Deshi Lactobacillus and
Viable count is 5 × 109-8×109The mixed-culture medium of bacillus acidophilus, be 5 × 10 with viable count9-8×109Lichens bud
Spore bacillus and viable count are 5 × 109-8×109The mixed-culture medium of Saccharomyces paradoxus bacterium mix according to the volume ratio of 7:5 and get final product.
As further improvement of these options, described expanded pellet diet is grass carp parent fishes special swelling feedstuff, at its table
Face is equivalent to the microbial ecological agent of its weight 1.5-2% by paint finishing spraying, is then fed into cooler cooling, after completing again
Be equivalent to the prevention enteritis preparation of its weight 0.6-0.8% by vacuum method at expanded pellet diet surface spraying, after cooling, control product
Water content is 10-12%, obtains finished product packing.
The using method of a kind of micro-ecological feed preventing grass carp parent fishes enteritis, at grass carp parent fishes cultivation stage, every 5 days
Use described micro-ecological feed to substitute conventional feed once, use described micro-ecological feed to feed grass carp parent fishes two continuously every time
My god；When finding the ill sign of enteritis, use described micro-ecological feed continuously until the ill sign of enteritis disappears.
Wherein, the ill sign of described enteritis is: slow in action, appetite is poor, abdominal part swelling etc..
The present invention have the advantage that compared to existing technology in the present invention micro-ecological feed can antagonism pathogen, improve
Animal body immunity, makes full use of the mutuality of interest between strain, obtains strong stress resistance, effectively maintains environment in animal intestinal
Microecological balance, is sprayed on expanded granular feed surface by microbial ecological agent and prevention enteritis preparation so that it is can be the most effective
Act on grass carp intestinal, simultaneously microbial ecological agent and prevention enteritis preparation synergism, it is possible to for the intestinal condition of Ctenopharyngodon idellus
Play the effect improving regulation, it is possible to effectively play prevention and the effect for the treatment of, greatly reduce the enteritis prevalence of Ctenopharyngodon idellus with dead
Die rate.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
A kind of micro-ecological feed preventing grass carp parent fishes enteritis, in expanded pellet diet surface spraying microbial ecological agent and prevention enteritis system
Agent；
Wherein, microbial ecological agent comprises: streptococcus faecalis viable count is 1 × 109-2×109Individual/ml, Deshi Lactobacillus viable count is
5×108-1×109Individual/ml, live lactobacillus acidophilus number is 3 × 109-4×109Individual/ml, Bacillus licheniformis viable count is 1
×109-4×109Individual/ml, Saccharomyces paradoxus bacterium viable count is 1 × 109-4×109Individual/ml；
Wherein, prevention enteritis preparation is mixed by the raw material of following weight portion: sulfoethyl zymosan 5, and cryptoxanthin 1 is sub-
Spermine 1, Rhizoma Alismatis extract 3, Freund adjuvant 20；
Wherein, in sulfoethyl zymosan, the substitution value of sulfoethyl is 0.736-0.852.
Wherein, described sulfoethyl zymosan is under alkaline system, by 2-chloroethyl sodium sulfonate and the zymosan system of reacting
Standby；Preparation condition is: zymosan is 2:5 with the weight ratio of 2-chloroethyl sodium sulfonate, the percentage by weight of sodium hydroxide solution
Being 35%, during heating, microwave frequency is 280W, and the microwave time is 36 minutes.
Wherein, the preparation method of described microbial ecological agent comprises the following steps:
(1) preparing the mixed-culture medium of streptococcus faecalis, Deshi Lactobacillus and bacillus acidophilus, its inoculum concentration is culture volume
1%, after twice cultivation and get final product；The culture medium used contains the composition of following percentage by weight: glucose 2.4%, Semen Maydis
Slurry 1.6%, Radix Raphani lyophilized powder 0.6%, bean milk 1.8%, carbamide 0.5%, hemoglobin 01%, calcium chloride 0.08%, potassium dihydrogen phosphate
0.02%, ammonium sulfate 0.06%, magnesium sulfate 0.02%, surplus are water, and pH value is 7；
(2) preparing Bacillus licheniformis and the mixed-culture medium of Saccharomyces paradoxus bacterium, its inoculum concentration is the 0.8% of culture volume, warp
After crossing twice cultivation and get final product；The culture medium used contains the composition of following percentage by weight: glucose 1.6, Semen Maydis pulp 1.4%,
Radix Raphani lyophilized powder 1%, bean milk 2.2%, carbamide 0.2%, hemoglobin 1.5%, calcium chloride 0.03%, potassium dihydrogen phosphate 0.06%, ammonium sulfate
0.08%, magnesium sulfate 0.01%, surplus are water, and pH value is 7.4；
(3) it is 6 × 10 by viable count9-1×1010Streptococcus faecalis, viable count be 3 × 109-6×109Deshi Lactobacillus and
Viable count is 5 × 109-8×109The mixed-culture medium of bacillus acidophilus, be 5 × 10 with viable count9-8×109Lichens bud
Spore bacillus and viable count are 5 × 109-8×109The mixed-culture medium of Saccharomyces paradoxus bacterium mix according to the volume ratio of 7:5 and get final product.
Wherein, described expanded pellet diet is grass carp parent fishes special swelling feedstuff, on its surface by paint finishing spraying quite
In the microbial ecological agent of its weight 1.8%, it is then fed into cooler cooling, passes through vacuum method after completing again on expanded pellet diet surface
Spraying is equivalent to the prevention enteritis preparation of its weight 0.6%, and controlling product water content after cooling is 10-12%, obtains finished product packing
?.
The using method of a kind of micro-ecological feed preventing grass carp parent fishes enteritis, at grass carp parent fishes cultivation stage, every 5 days
Use described micro-ecological feed to substitute conventional feed once, use described micro-ecological feed to feed grass carp parent fishes two continuously every time
My god；When finding the ill sign of enteritis, use described micro-ecological feed continuously until the ill sign of enteritis disappears.
Wherein, the ill sign of described enteritis is: slow in action, appetite is poor, abdominal part swelling etc..
Use feedstuff in the present invention to feed grass carp parent fishes, make parent fish enteritis prevalence be reduced to less than 3%, feed coefficient
It is reduced to 2.06, survival rate to about 98.6%, remarkable in economical benefits.
Claims (5)
1. the micro-ecological feed preventing grass carp parent fishes enteritis, it is characterised in that in expanded pellet diet surface spraying Tiny ecosystem system
Agent and prevention enteritis preparation；
Wherein, microbial ecological agent comprises: streptococcus faecalis viable count is 1 × 109-2×109Individual/ml, Deshi Lactobacillus viable count is 5
×108-1×109Individual/ml, live lactobacillus acidophilus number is 3 × 109-4×109Individual/ml, Bacillus licheniformis viable count is 1 ×
109-4×109Individual/ml, Saccharomyces paradoxus bacterium viable count is 1 × 109-4×109Individual/ml；
Wherein, prevention enteritis preparation is mixed by the raw material of following weight portion: sulfoethyl zymosan 5, and cryptoxanthin 1 is sub-
Spermine 1, Rhizoma Alismatis extract 3, Freund adjuvant 20；
Wherein, in sulfoethyl zymosan, the substitution value of sulfoethyl is 0.736-0.852.
A kind of micro-ecological feed preventing grass carp parent fishes enteritis the most as claimed in claim 1, it is characterised in that described sulfoethyl ferment
Female polysaccharide, under alkaline system, is reacted preparation by 2-chloroethyl sodium sulfonate with zymosan；Preparation condition is: zymosan and 2-
The weight ratio of chloroethyl sodium sulfonate is 2:5, and the percentage by weight of sodium hydroxide solution is 35%, and during heating, microwave frequency is 280W,
The microwave time is 36 minutes.
A kind of micro-ecological feed preventing grass carp parent fishes enteritis the most as claimed in claim 1, it is characterised in that described Tiny ecosystem system
The preparation method of agent comprises the following steps:
(1) preparing the mixed-culture medium of streptococcus faecalis, Deshi Lactobacillus and bacillus acidophilus, its inoculum concentration is culture volume
1%, after twice cultivation and get final product；The culture medium used contains the composition of following percentage by weight: glucose 2.2-2.5%,
Semen Maydis pulp 1.5-1.8%, Radix Raphani lyophilized powder 0.4-0.8%, bean milk 1.5-2.2%, carbamide 0.2-0.6%, hemoglobin 0.7-1.2%,
Calcium chloride 0.06-0.08%, potassium dihydrogen phosphate 0.02-0.04%, ammonium sulfate 0.05-0.08%, magnesium sulfate 0.01-0.02%, surplus
For water, pH value is 6.8-7.2；
(2) preparing Bacillus licheniformis and the mixed-culture medium of Saccharomyces paradoxus bacterium, its inoculum concentration is the 0.8% of culture volume, warp
After crossing twice cultivation and get final product；The culture medium used contains the composition of following percentage by weight: glucose 1.6-1.8%, Semen Maydis pulp
1.2-1.5%, Radix Raphani lyophilized powder 0.8-1.2%, bean milk 1.5-2.2%, carbamide 0.1-0.3%, hemoglobin 1.3-1.5%, calcium chloride
0.02-0.04%, potassium dihydrogen phosphate 0.05-0.08%, ammonium sulfate 0.05-0.08%, magnesium sulfate 0.01-0.02%, surplus are water, pH
Value is 7.2-7.4；
(3) it is 6 × 10 by viable count9-1×1010Streptococcus faecalis, viable count be 3 × 109-6×109Deshi Lactobacillus and work
Bacterium number is 5 × 109-8×109The mixed-culture medium of bacillus acidophilus, be 5 × 10 with viable count9-8×109Lichens spore
Bacillus and viable count are 5 × 109-8×109The mixed-culture medium of Saccharomyces paradoxus bacterium mix according to the volume ratio of 7:5 and get final product.
4. a kind of micro-ecological feed preventing grass carp parent fishes enteritis as described in any one in claim 1-3, it is characterised in that
Described expanded pellet diet is grass carp parent fishes special swelling feedstuff, is equivalent to its weight 1.5-2% on its surface by paint finishing spraying
Microbial ecological agent, be then fed into cooler cooling, be equivalent to it by vacuum method at expanded pellet diet surface spraying again after completing
The prevention enteritis preparation of weight 0.6-0.8%, controlling product water content after cooling is 10-12%, obtains finished product packing.
5. the using method of the micro-ecological feed of a prevention grass carp parent fishes enteritis as claimed in claim 4, it is characterised in that
Grass carp parent fishes cultivation stage, used described micro-ecological feed to substitute conventional feed once every 5 days, used described Tiny ecosystem every time
Feedstuff feeds grass carp parent fishes two days continuously；When finding the ill sign of enteritis, continuously use described micro-ecological feed until enteritis
Ill sign disappears.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610923390.9A CN106260792A (en) | 2016-10-22 | 2016-10-22 | A kind of micro-ecological feed preventing grass carp parent fishes enteritis and using method thereof |
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| CN201610923390.9A CN106260792A (en) | 2016-10-22 | 2016-10-22 | A kind of micro-ecological feed preventing grass carp parent fishes enteritis and using method thereof |
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| CN201610923390.9A Pending CN106260792A (en) | 2016-10-22 | 2016-10-22 | A kind of micro-ecological feed preventing grass carp parent fishes enteritis and using method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771402A (en) * | 2019-03-19 | 2019-05-21 | 浙江工业大学 | Spermidine is damaged the application in drug in preparation treatment intestinal barrier function |
| CN111820316A (en) * | 2020-06-30 | 2020-10-27 | 新疆天康饲料有限公司 | Fermented puffed pellet feed for early stage of overwintering grass carp culture and preparation method thereof |
| CN112293605A (en) * | 2019-07-25 | 2021-02-02 | 领袖生物技术农业会社法人 | EP puffed fermented compound feed with effects of promoting digestion, immunocompetence and growth of fish and preparation method thereof |
-
2016
- 2016-10-22 CN CN201610923390.9A patent/CN106260792A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771402A (en) * | 2019-03-19 | 2019-05-21 | 浙江工业大学 | Spermidine is damaged the application in drug in preparation treatment intestinal barrier function |
| CN112293605A (en) * | 2019-07-25 | 2021-02-02 | 领袖生物技术农业会社法人 | EP puffed fermented compound feed with effects of promoting digestion, immunocompetence and growth of fish and preparation method thereof |
| CN111820316A (en) * | 2020-06-30 | 2020-10-27 | 新疆天康饲料有限公司 | Fermented puffed pellet feed for early stage of overwintering grass carp culture and preparation method thereof |
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Application publication date: 20170104 |