CN106260594A - A kind of pre-mixing feed additive improving sow reproductive performance - Google Patents
A kind of pre-mixing feed additive improving sow reproductive performance Download PDFInfo
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Abstract
The invention discloses a kind of pre-mixing feed additive improving sow reproductive performance, this pre-mixing feed additive is mainly by composite antibacterial peptide moiety, form including Catfish antimicrobial peptide mutant, sea hare antibacterial peptide mutant, maduca sexta antibacterial peptide mutant, enterococcus faecalis and adjuvant, it is adaptable to sow whole process uses.Method of the present invention can significantly improve swinery intestinal microecology, it is obviously improved sow immunity, and then raising sow reproductive performance, the most just it is to increase tire all total young number, the tire all strong young number of young number, tire all alive, and compared with the existing methods, it is also possible to many nutrition is provided, improve newborn quality and the quantity of sow, strengthen the body constitution of piglet, reduce breeding cycle.
Description
Technical field
The invention belongs to the technical field of animal feed, be specifically related to a kind of premixed feed improving sow reproductive performance
Additive.
Background technology
The development produced along with intensive, large scale of pig farm and management development, manufacturer is in order to realize sow group
The high efficiency produced, requires more and more higher to the quality of swinery, kind, health status farrowing rate.By improving management means, make
Swinery, under the strict production schedule controls, carries out full production, makes the abnormal superseded ratio of swinery relative therefrom
Increase.At present in Swine Production, the difficult problem perplexing the people that raises pigs is: litter size is few, causes the reproductive capacity on pig farm to decline, sow group
Supplement difficulty.If we add sow livestock on hand, improve farrowing nest number, the feeding cost of sow certainly will be increased, but also cause
The field of sow house is nervous；It is not added with the livestock on hand of sow on the contrary, childbirth, child care, it is bred as the field relative free of house, causes waste
And increase cost.Sow occupies critically important status in Swine Production, and whole Swine Production efficiency is extremely closed by the health of sow
Important.Sow gestation to be experienced, suckling and nonpregnant three cycles and breedings in the normal breeding cycle, farrow and wean three
Link, any link goes wrong, and can reduce sow reproductive performance, reduces production efficiency.
In these links, in the immune level of sow and digestive tract, colony balance plays an important role, sow digestive tract
The unbalance meeting of microorganism causes the generation of animal intestinal disease, and such as constipation, diarrhoea etc., and the decline of immunity causes multiple disease
Occur and sow is in sub-health state.
Many studies have shown that, sow feed adds probiotic bacteria and antibacterial peptide, to Milking ability, milk quality, wean son
The reproductive performances such as pig body weight have certain facilitation.Gorgeous equality is raised 7d antenatal to multiparity and first farrowing sow every day every respectively
Feeding microbial ecological agent, it was found that Suprapubic arch sling group and first farrowing sow group, piglet growth speed, piglet survival ratio significantly improve.
The research such as Li Bo shows, adds cecropin antimicrobial peptides and can significantly improve sow in sow antenatal gestation material and postpartum breastfeeding material
Reproductive performance and the growth performance of piglet, improve the health level of piglet.
People are a large amount of in feedstuff adds chemicals and antibiotic, to improving fertility of sow and preventing disease to achieve
Certain effect, but its effect is not satisfactory, and easily drug residue problem, health is adversely affected.
Summary of the invention
It is an object of the invention to provide a kind of pre-mixing feed additive improving sow reproductive performance.
The technical solution used in the present invention is:
A kind of pre-mixing feed additive, described feed additive percentage meter by weight contains following components: 0.5%～
10% Catfish antimicrobial peptide mutant, 0.5%～10% sea hare antibacterial peptide mutant Da-B2M, 0.5%～10% maduca sexta resist
Bacterium peptide mutant Ms-mcM and 0.5%～10% enterococcus faecalis agent.
Further, described feed additive percentage meter by weight contains following components: 0.5%～10% Catfish antimicrobial peptide
Mutant, 0.5%～10% sea hare antibacterial peptide mutant Da-B2M, 0.5%～10% maduca sexta antibacterial peptide mutant Ms-
McM, 0.5%～10% enterococcus faecalis agent, surplus is adjuvant.
Further, described feed additive percentage meter by weight contains following components: 2.0%～6.0% Silurus asotus fish is antibacterial
Peptide mutant, 1.0%～2.0% sea hare antibacterial peptide mutant Da-B2M, 1.0%～2.0% maduca sexta antibacterial peptide mutant
Ms-mcM, 0.5%～10% enterococcus faecalis agent, surplus is adjuvant.
Further, described Catfish antimicrobial peptide mutant, sea hare antibacterial peptide mutant Da-B2M and maduca sexta antibacterial peptide
The mass ratio of mutant is 4:1:1.
Further, described feed additive percentage meter by weight contains following components: 4.0% Catfish antimicrobial peptide is suddenlyd change
Body, 1.0% sea hare antibacterial peptide mutant Da-B2M, 1.0% maduca sexta antibacterial peptide mutant Ms-mcM, 0.5% dung intestinal ball and
93.5% adjuvant.
Further, the aminoacid sequence of described Catfish antimicrobial peptide mutant be KGRGKQGGKVRKSS (SEQ ID NO:
1)。
Further, the aminoacid sequence of described sea hare antibacterial peptide mutant Da-B2M is SHISCGEALHKCMASHSKPF
SCSCKFHMYLQQQ (SEQ ID NO:2).
Further, the aminoacid sequence of described maduca sexta antibacterial peptide mutant Ms-mcM is GKIPIGAIKQAGKAIG
KGLRAINIASTTHDVVSFFRPKKKRH (SEQ ID NO:3).
Further, in described enterococcus faecalis agent, the concentration of enterococcus faecalis is 2 × 109～3 × 109CFU/g。
Further, described adjuvant contains antioxidant, compound vitamin, mineral element additive, cassiri, ferment
At least one in female culture, zeolite powder.
The invention has the beneficial effects as follows:
1) pre-mixing feed additive of the present invention contains specific composite antibacterial peptide moiety, i.e. Catfish antimicrobial peptide mutant,
Sea hare (Dolabella auricularia) antibacterial peptide (Dolabellanin B2) mutant Da-B2M, maduca sexta
(Manduca sexta) antibacterial peptide MS moricin mutant Ms-mcM；Simultaneously possibly together with specific enterococcus faecalis
(Enterococcus faecium) GLM105, deposit number is CGMCC No.11155, it is adaptable to sow whole process uses；This
The mutant of the specific antibacterial peptide of bright selection and the combination application of specific bacterial strain, serve more preferable effect, is more beneficial for improving mother
Swine reproduction performance, is the most just to increase tire all total young number, tire and all lives all strong young number of young number, tire, and with existing method
Compare, it is also possible to many nutrition is provided, improve newborn quality and the quantity of sow, strengthen the body constitution of piglet, can shorten simultaneously
Feeding time and scale of feeding, reduce production cost.
2) present invention uses specific antibacterial peptide mutant and specific bacterial strain can improve effect of Swinery immunity power, promote sow
The function of reproductive capacity, and compare traditional drugs physical property additive, has the many merits such as noresidue, production cost be relatively low.
3) present invention is according to features such as the different antibacterial characteristics of antibacterial peptide, immunocompetences, specific compounding dissimilar
Antibacterial peptide, obtain recombinant antimicrobial peptide；It is aided with pig endogenous bacterial strain (enterococcus faecalis) simultaneously, ultimately forms recombinant antimicrobial peptide, benefit
The compound premixing feed additives that raw bacterium is compounding.Test result indicate that, compound premixing feed additives can significantly improve sow reproductive ability
Energy (young number etc. is all good for by tire all total young number, tire young number, tire all alive), may be provided for many nutrition simultaneously, improves sow
Breast quality and quantity, strengthen the body constitution of piglet, reduces the feeding piglet cycle.Method low cost of the present invention, the cycle is short, makes
With convenient, reality application is made great sense.
Detailed description of the invention
A kind of pre-mixing feed additive, described feed additive percentage meter by weight contains following components: 0.5%～
10% Catfish antimicrobial peptide mutant, 0.5%～10% sea hare antibacterial peptide mutant Da-B2M, 0.5%～10% maduca sexta resist
Bacterium peptide mutant Ms-mcM and 0.5%～10% enterococcus faecalis agent.
Preferably, described feed additive percentage meter by weight contains following components: 0.5%～10% Catfish antimicrobial peptide is dashed forward
Variant, 0.5%～10% sea hare antibacterial peptide mutant Da-B2M, 0.5%～10% maduca sexta antibacterial peptide mutant Ms-mcM,
0.5%～10% enterococcus faecalis agent, surplus is adjuvant.
Preferably, described feed additive percentage meter by weight contains following components: 2.0%～6.0% Catfish antimicrobial peptide
Mutant, 1.0%～2.0% sea hare antibacterial peptide mutant Da-B2M, 1.0%～2.0% maduca sexta antibacterial peptide mutant Ms-
McM, 0.5%～10% enterococcus faecalis agent, surplus is adjuvant.
Preferably, described Catfish antimicrobial peptide mutant, sea hare antibacterial peptide mutant Da-B2M and maduca sexta antibacterial peptide are prominent
The mass ratio of variant is 4:1:1.
Preferably, described feed additive percentage meter by weight contains following components: 4.0% Catfish antimicrobial peptide mutant,
1.0% sea hare antibacterial peptide mutant Da-B2M, 1.0% maduca sexta antibacterial peptide mutant Ms-mcM, 0.5% dung intestinal ball and
93.5% adjuvant.
Preferably, the aminoacid sequence of described Catfish antimicrobial peptide mutant is KGRGKQGGKVRKSS (SEQ ID NO:1).
Preferably, the aminoacid sequence of described sea hare antibacterial peptide mutant Da-B2M is SHISCGEALHKCMASHSKPFSC
SCKFHMYLQQQ (SEQ ID NO:2).
Preferably, the aminoacid sequence of described maduca sexta antibacterial peptide mutant Ms-mcM is GKIPIGAIKQAGKAIGKG
LRAINIASTTHDVVSFFRPKKKRH (SEQ ID NO:3).
Preferably, in described enterococcus faecalis agent, the concentration of enterococcus faecalis is 2 × 109～3 × 109CFU/g。
Preferably, the Classification And Nomenclature of described enterococcus faecalis is (Enterococcus faecium) GLM105, deposit number
For CGMCC No.11155.
Preferably, described adjuvant contains antioxidant, compound vitamin, mineral element additive, cassiri, yeast
At least one in culture, zeolite powder.
It is furthermore preferred that described adjuvant is composed of the following components: 53%～55% cassiri, 32%～33% yeast training
Support thing, 9%～11% fermented bean cake, 0.2%～0.4% compound vitamin, 0.15%～0.25% mineral element additive,
1.0%～2.0% zeolite powder, 1.3%～1.6% calcium hydrogen phosphate, described percent is mass percent.
The using method of any of the above-described described pre-mixing feed additive, in arm's length basis daily ration add 0.1%～
The pre-mixing feed additive of 0.5%.
Below in conjunction with specific embodiment, the present invention is further illustrated, but is not limited thereto.
Embodiment 1～4 one kind of pre-mixing feed additive
The formula of the pre-mixing feed additive prepared by embodiment 1～4 and the formula of corresponding comparative example 1～3 are as follows
Shown in table 1 (in table, percent all represents mass percent).
The formula of the pre-mixing feed additive prepared by table 1 embodiment 1～4 and the formula of corresponding comparative example 1～3
In upper table, the aminoacid sequence of described Catfish antimicrobial peptide mutant is KGRGKQGGKVRKSS (SEQ ID NO:1).
The aminoacid sequence of described sea hare antibacterial peptide mutant Da-B2M is SHISCGEALHKCMASHSKPFSCSCKFHMY
LQQQ (SEQ ID NO:2).
The aminoacid sequence of described maduca sexta antibacterial peptide mutant Ms-mcM is GKIPIGAIKQAGKAIGKGLRAINIA
STTHDVVSFFRPKKKRH (SEQ ID NO:3).
In described enterococcus faecalis agent, the concentration of enterococcus faecalis is 2.5 × 109CFU/g；The Classification And Nomenclature of described enterococcus faecalis
For (Enterococcus faecium) GLM105, deposit number is that CGMCC No.11155 (sees patent document
CN105132321A)。
The formula of described adjuvant is: 54% cassiri, 32.5% yeast culture, 10% fermented bean cake, 0.3% multiple
Closing vitamin, 0.2% mineral element additive, 1.5% zeolite powder, 1.5% calcium hydrogen phosphate, described percent is percent mass
Number.
In above-mentioned table, the preparation method of pre-mixing feed additive (hereinafter referred to as premix material) is in stirring by all raw materials
After machine is sufficiently stirred for.
Embodiment 5 plant application experiment
Experimental technique: this experimental example is carried out in pig plant, randomly selects 400 healthy sows, is randomly divided into 8
Group, often group 50, wherein the 1st group is experimental group to the 7th group, is separately added into the pre-mixing feed additive of different formulations in table 1
Raising, the 8th group is matched group.Experimental group sow adds 0.3% premixed feed after breeding to wean in feedstuff
Additive, matched group to before wean, normally feeds at feedstuff, monthly adds week age after breeding, and record test group and
Matched group sow health status, farrowing head number, piglet Birth weight, weaning weight and health status.Experiment detailed design such as table 2.
Table 2 daily ration structure
Experimental period: start after insemination of sows to piglet 28 age in days to terminate, amount to 142 days.
Experimental result and conclusion:
(1) pre-mixing feed additive characterizes the impact of health status to sow
As shown in table 3, during testing, 7 test group are not notable with the equal feed intake of head of matched group, sickness rate difference,
Various symptoms also no significant difference.Result shows, pre-mixing feed additive of the present invention (premix material) is improving the sign of sow
In health status the most notable with the difference of the premix material of comparative example and matched group.
Table 3 sow characterizes health status
Note: different lowercase alphabets show that significant difference (P ＜ 0.05) capitalization represents that difference is extremely notable (P ＜ 0.01).
(2) pre-mixing feed additive is on number born of sow, the weak tire of piglet, stillborn fetus, the impact of mummy
As shown in table 4, weak young number than matched group few 19 in using 1 group of embodiment 1 pre-mixing feed additive；Stillborn fetus
Number few 8 than matched group, mummy number than matched group few 12；Total sodium selenite number is higher than matched group 104；
Weak young number than matched group few 16 in using 2 groups of embodiment 2 pre-mixing feed additive；Stillborn fetus number compares matched group
Few 7, mummy number than matched group few 11；Total sodium selenite number is higher than matched group 95；
Weak young number than matched group few 17 in using 3 groups of embodiment 3 pre-mixing feed additive；Stillborn fetus number compares matched group
Few 6, mummy number than matched group few 9；Experimental group total sodium selenite number is higher than matched group 93；
Weak young number than matched group few 15 in using 4 groups of embodiment 4 pre-mixing feed additive；Stillborn fetus number compares matched group
Few 5, mummy number than matched group few 9；Experimental group total sodium selenite number is higher than matched group 93；
Weak young number than matched group few 15 in using 5 groups of comparative example 1 premix material；Stillborn fetus number few 3 than matched group, wood are
Her number than matched group few 7；Experimental group total sodium selenite number is higher than matched group 79；
Weak young number than matched group few 8 in using 6 groups of comparative example 2 premix material；Stillborn fetus number few 4 than matched group, wood are
Her number than matched group few 8；Experimental group total sodium selenite number is higher than matched group 69；
Weak young number than matched group few 12 in using 7 groups of comparative example 3 premix material；Stillborn fetus number few 2 than matched group, wood are
Her number than matched group few 8；Experimental group total sodium selenite number is higher than matched group 75.
Summary result, pre-mixing feed additive of the present invention is to number born of sow, the weak tire of piglet, stillborn fetus, mummy
More notable Deng the impact of sow reproductive performance, the reproductive performance of the mixed fodder additive on sow of the present invention under different formulations
All it is obviously improved, and its effect is also apparently higher than the formula in comparative example, especially use embodiment 1 pre-mixing feed additive
Time, sow reproductive performance is promoted best results,.
Table 4 number born of sow, the weak tire of piglet, stillborn fetus, mummy situation
| Item compared | 1 group | 2 groups | 3 groups | 4 groups | 5 groups | 6 groups | 7 groups | Matched group |
| Total healthy young number | 630 | 621 | 619 | 619 | 605 | 595 | 601 | 526 |
| Total weak young number | 14 | 17 | 16 | 18 | 18 | 25 | 21 | 33 |
| Total stillborn fetus number | 10 | 11 | 12 | 13 | 15 | 14 | 16 | 18 |
| Total mummy number | 6 | 7 | 9 | 9 | 11 | 10 | 10 | 18 |
(3) pre-mixing feed additive is on sows farrowing head number, suckling pig Birth weight, the impact of weaning weight
As shown in table 5, all litter sizes in 1 group of embodiment 1 pre-mixing feed additive are used to be higher than 1.3 (P of matched group
< 0.01), piglet Birth weight be higher than matched group 0.23kg (P < 0.01), 25 age in days weaned piglets heavily higher than matched group 0.90kg (P <
0.05)；
All litter sizes in 2 groups of embodiment 2 pre-mixing feed additive are used to be higher than matched group 1.22 (P < 0.01), young
Pig Birth weight is higher than matched group 0.14kg (P < 0.05), and 25 age in days weaned piglets are heavily higher than matched group 0.69kg (P < 0.05)；
All litter sizes in 3 groups of embodiment 3 pre-mixing feed additive are used to be higher than matched group 1.22 (P < 0.01), young
Pig Birth weight is higher than matched group 0.15kg (P < 0.05), and 25 age in days weaned piglets are heavily higher than matched group 0.78kg (P < 0.05)；
All litter sizes in 4 groups of embodiment 4 pre-mixing feed additive are used to be higher than matched group 1.28 (P < 0.01), young
Pig Birth weight is higher than matched group 0.19kg (P < 0.05), and 25 age in days weaned piglets are heavily higher than matched group 0.80kg (P < 0.05)；
All litter sizes in 5 groups of comparative example 1 premix material are used to be higher than matched group higher than matched group 1.08, piglet Birth weight
0.09kg, 25 age in days weaned piglets are heavily higher than matched group 0.55kg；
All litter sizes in 6 groups of comparative example 2 premix material are used to be higher than matched group higher than matched group 0.98, piglet Birth weight
0.12kg, 25 age in days weaned piglets are heavily higher than matched group 0.78kg；
All litter sizes in 7 groups of comparative example 3 premix material are used to be higher than matched group higher than matched group 1.06, piglet Birth weight
0.17kg, 25 age in days weaned piglets are heavily higher than matched group 0.76kg.
Table 5 sows farrowing head number, suckling pig Birth weight, weaning weight situation
Note: a represents significant difference (P ＜ 0.05), and A represents that difference is extremely notable (P ＜ 0.01).
Summary result, pre-mixing feed additive of the present invention is to farrowing head number, suckling pig Birth weight, weaning weight etc.
The impact of sow reproductive performance is more notable, and the reproductive performance of the mixed fodder additive on sow of the present invention under different formulations is equal
It is obviously improved, and its effect is also apparently higher than the formula in comparative example, especially use embodiment 1 pre-mixing feed additive
Time, sow reproductive performance is promoted best results, all farrowing head number reach 13.20 ± 0.4, suckling pig birth weigh 1.51 ±
0.13kg, wean weigh 7.37 ± 0.24kg.
6 one kinds of pre-mixing feed additives of embodiment
The present embodiment pre-mixing feed additive percentage meter by weight contains following components: 10% Catfish antimicrobial peptide is suddenlyd change
Body, 0.5% sea hare antibacterial peptide mutant Da-B2M, 10% maduca sexta antibacterial peptide mutant Ms-mcM and 10% enterococcus faecalis
Agent, surplus is adjuvant；
The aminoacid sequence of described Catfish antimicrobial peptide mutant is KGRGKQGGKVRKSS (SEQID NO:1).
The aminoacid sequence of described sea hare antibacterial peptide mutant Da-B2M is SHISCGEALHKCMASHSKPFSCSCKFHMY
LQQQ (SEQ ID NO:2).
The aminoacid sequence of described maduca sexta antibacterial peptide mutant Ms-mcM is GKIPIGAIKQAGKAIGKGLRAINIA
STTHDVVSFFRPKKKRH (SEQ ID NO:3).
In described enterococcus faecalis agent, the concentration of enterococcus faecalis is 2.5 × 109CFU/g。
The formula of described adjuvant is: 54% cassiri, 32.5% yeast culture, 10% fermented bean cake, 0.3% multiple
Closing vitamin, 0.2% mineral element additive, 1.5% zeolite powder, 1.5% calcium hydrogen phosphate, described percent is percent mass
Number.
7 one kinds of pre-mixing feed additives of embodiment
The present embodiment pre-mixing feed additive percentage meter by weight contains following components: 0.5% Catfish antimicrobial peptide is suddenlyd change
Body, 10% sea hare antibacterial peptide mutant Da-B2M, 0.5% maduca sexta antibacterial peptide mutant Ms-mcM and 5% enterococcus faecalis agent,
Surplus is adjuvant；
The aminoacid sequence of described Catfish antimicrobial peptide mutant is KGRGKQGGKVRKSS (SEQ ID NO:1).
The aminoacid sequence of described sea hare antibacterial peptide mutant Da-B2M is SHISCGEALHKCMASHSKPFSCSCKFHMY
LQQQ (SEQ ID NO:2).
The aminoacid sequence of described maduca sexta antibacterial peptide mutant Ms-mcM is GKIPIGAIKQAGKAIGKGLRAINIA
STTHDVVSFFRPKKKRH (SEQ ID NO:3).
In described enterococcus faecalis agent, the concentration of enterococcus faecalis is 2.5 × 109CFU/g。
The formula of described adjuvant is: 54% cassiri, 32.5% yeast culture, 10% fermented bean cake, 0.3% multiple
Closing vitamin, 0.2% mineral element additive, 1.5% zeolite powder, 1.5% calcium hydrogen phosphate, described percent is percent mass
Number.
8 one kinds of pre-mixing feed additives of embodiment
The present embodiment pre-mixing feed additive percentage meter by weight contains following components: 6.0% Catfish antimicrobial peptide is suddenlyd change
Body, 2.0% sea hare antibacterial peptide mutant Da-B2M, 1.0% maduca sexta antibacterial peptide mutant Ms-mcM, 4% enterococcus faecalis agent,
Surplus is adjuvant.
The aminoacid sequence of described Catfish antimicrobial peptide mutant is KGRGKQGGKVRKSS (SEQ ID NO:1).
The aminoacid sequence of described sea hare antibacterial peptide mutant Da-B2M is SHISCGEALHKCMASHSKPFSCSCKFHMY
LQQQ (SEQ ID NO:2).
The aminoacid sequence of described maduca sexta antibacterial peptide mutant Ms-mcM is GKIPIGAIKQAGKAIGKGLRAINIA
STTHDVVSFFRPKKKRH (SEQ ID NO:3).
The concentration of described enterococcus faecalis is 3 × 109CFU/g。
The formula of described adjuvant is: 54% cassiri, 32.5% yeast culture, 10% fermented bean cake, 0.3% multiple
Closing vitamin, 0.2% mineral element additive, 1.5% zeolite powder, 1.5% calcium hydrogen phosphate, described percent is percent mass
Number.
9 one kinds of pre-mixing feed additives of embodiment
The present embodiment pre-mixing feed additive percentage meter by weight contains following components: 2.0% Catfish antimicrobial peptide is suddenlyd change
Body, 1.0% sea hare antibacterial peptide mutant Da-B2M, 2.0% maduca sexta antibacterial peptide mutant Ms-mcM, 10% enterococcus faecalis
Agent, surplus is adjuvant.
The aminoacid sequence of described Catfish antimicrobial peptide mutant is KGRGKQGGKVRKSS (SEQ ID NO:1).
The aminoacid sequence of described sea hare antibacterial peptide mutant Da-B2M is SHISCGEALHKCMASHSKPFSCSCKFHMY
LQQQ (SEQ ID NO:2).
The aminoacid sequence of described maduca sexta antibacterial peptide mutant Ms-mcM is GKIPIGAIKQAGKAIGKGLRAINIA
STTHDVVSFFRPKKKRH (SEQ ID NO:3).
In described enterococcus faecalis agent, the concentration of enterococcus faecalis is 2 × 109CFU/g。
The formula of described adjuvant is: 54% cassiri, 32.5% yeast culture, 10% fermented bean cake, 0.3% multiple
Closing vitamin, 0.2% mineral element additive, 1.5% zeolite powder, 1.5% calcium hydrogen phosphate, described percent is percent mass
Number.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not by above-described embodiment
Limit, the change made under other any spirit without departing from the present invention and principle, modify, substitute, combine, simplify,
All should be the substitute mode of equivalence, within being included in protection scope of the present invention.
<110>Jiangsu Shang Bao animal husbandry company limited
Guangzhou Glam Biotechnology Co., Ltd.
<120>a kind of pre-mixing feed additive improving sow reproductive performance
<130>
<160> 3
<170> PatentIn version 3.5
<210> 1
<211> 14
<212> PRT
<213>artificial sequence
<400> 1
Lys Gly Arg Gly Lys Gln Gly Gly Lys Val Arg Lys Ser Ser
1 5 10
<210> 2
<211> 33
<212> PRT
<213>artificial sequence
<400> 2
Ser His Ile Ser Cys Gly Glu Ala Leu His Lys Cys Met Ala Ser His
1 5 10 15
Ser Lys Pro Phe Ser Cys Ser Cys Lys Phe His Met Tyr Leu Gln Gln
20 25 30
Gln
Claims (10)
1. a pre-mixing feed additive, it is characterised in that: described feed additive percentage meter by weight contains following components:
0.5% ~ 10% Catfish antimicrobial peptide mutant, 0.5% ~ 10% sea hare antibacterial peptide mutant Da-B2M, 0.5% ~ 10% Nicotiana tabacum L. sky
Moth antibacterial peptide mutant Ms-mcM and 0.5% ~ 10% enterococcus faecalis agent.
A kind of pre-mixing feed additive the most according to claim 1, it is characterised in that: described feed additive is by weight
Percentage meter contains following components: 0.5% ~ 10% Catfish antimicrobial peptide mutant, 0.5% ~ 10% sea hare antibacterial peptide mutant Da-
B2M, 0.5% ~ 10% maduca sexta antibacterial peptide mutant Ms-mcM, 0.5% ~ 10% enterococcus faecalis agent, surplus is adjuvant.
A kind of pre-mixing feed additive the most according to claim 2, it is characterised in that: described feed additive is by weight
Percentage meter contains following components: 2.0% ~ 6.0% Catfish antimicrobial peptide mutant, 1.0% ~ 2.0% sea hare antibacterial peptide mutant Da-
B2M, 1.0% ~ 2.0% maduca sexta antibacterial peptide mutant Ms-mcM, 0.5% ~ 10% enterococcus faecalis agent, surplus is adjuvant.
A kind of pre-mixing feed additive the most according to claim 3, it is characterised in that: described Catfish antimicrobial peptide is suddenlyd change
The mass ratio of body, sea hare antibacterial peptide mutant Da-B2M and maduca sexta antibacterial peptide mutant is 4:1:1.
5. a pre-mixing feed additive, it is characterised in that: described feed additive percentage meter by weight contains following components:
4.0% Catfish antimicrobial peptide mutant, 1.0% sea hare antibacterial peptide mutant Da-B2M, 1.0% maduca sexta antibacterial peptide mutant
Ms-mcM, 0.5% dung intestinal ball and 93.5% adjuvant.
6. according to the arbitrary described a kind of pre-mixing feed additive of claim 1 ~ 5, it is characterised in that: described Catfish antimicrobial peptide
The aminoacid sequence of mutant is KGRGKQGGKVRKSS(SEQ ID NO:1).
7. according to the arbitrary described a kind of pre-mixing feed additive of claim 1 ~ 5, it is characterised in that: described sea hare antibacterial peptide
The aminoacid sequence of mutant Da-B2M is SHISCGEALHKCMASHSKPFSCSCKFHMYLQQQ(SEQ ID NO:2).
8. according to the arbitrary described a kind of pre-mixing feed additive of claim 1 ~ 5, it is characterised in that: described maduca sexta resists
The aminoacid sequence of bacterium peptide mutant Ms-mcM is GKIPIGAIKQAGKAIGKGLRAINIASTTHDVVSFFRPKKKRH(SEQ
ID NO:3).
9. according to the arbitrary described a kind of pre-mixing feed additive of claim 1 ~ 5, it is characterised in that: described enterococcus faecalis agent
The concentration of middle enterococcus faecalis is 2 × 109 ~3×109 CFU/g。
10. according to the arbitrary described a kind of pre-mixing feed additive of claim 1 ~ 5, it is characterised in that: described adjuvant contains
At least one in antioxidant, compound vitamin, mineral element additive, cassiri, yeast culture, zeolite powder.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL2032464A (en) * | 2021-07-23 | 2023-02-07 | Univ Liaocheng | Enterococcus faecium and use thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101164431A (en) * | 2007-10-08 | 2008-04-23 | 国家海洋局第一海洋研究所 | Feed additive with antibiotic function and application |
| CN102178112A (en) * | 2011-05-12 | 2011-09-14 | 比利美英伟营养饲料（深圳）有限公司 | Premix feed for relieving astriction of sows |
| CN102726618A (en) * | 2011-03-31 | 2012-10-17 | 成都世纪投资有限公司 | Composite premix for pigs |
| CN103288924A (en) * | 2012-10-23 | 2013-09-11 | 广州格拉姆生物科技有限公司 | Catfish antimicrobial peptide mutant and preparation method thereof |
| CN104605170A (en) * | 2013-11-04 | 2015-05-13 | 浙江金华神农农业科技有限公司 | Pig compound premix and pig feed |
| CN105132321A (en) * | 2015-09-02 | 2015-12-09 | 广州格拉姆生物科技有限公司 | Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method |
| CN105661039A (en) * | 2016-01-20 | 2016-06-15 | 四川中农致远生物科技有限公司 | Breeding sow feed additives and preparation method thereof |
-
2016
- 2016-08-12 CN CN201610667751.8A patent/CN106260594B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101164431A (en) * | 2007-10-08 | 2008-04-23 | 国家海洋局第一海洋研究所 | Feed additive with antibiotic function and application |
| CN102726618A (en) * | 2011-03-31 | 2012-10-17 | 成都世纪投资有限公司 | Composite premix for pigs |
| CN102178112A (en) * | 2011-05-12 | 2011-09-14 | 比利美英伟营养饲料（深圳）有限公司 | Premix feed for relieving astriction of sows |
| CN103288924A (en) * | 2012-10-23 | 2013-09-11 | 广州格拉姆生物科技有限公司 | Catfish antimicrobial peptide mutant and preparation method thereof |
| CN104605170A (en) * | 2013-11-04 | 2015-05-13 | 浙江金华神农农业科技有限公司 | Pig compound premix and pig feed |
| CN105132321A (en) * | 2015-09-02 | 2015-12-09 | 广州格拉姆生物科技有限公司 | Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method |
| CN105661039A (en) * | 2016-01-20 | 2016-06-15 | 四川中农致远生物科技有限公司 | Breeding sow feed additives and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL2032464A (en) * | 2021-07-23 | 2023-02-07 | Univ Liaocheng | Enterococcus faecium and use thereof |
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