CN106255873A - 一种进行自动补偿的方法、装置及相应的流式细胞分析仪 - Google Patents

一种进行自动补偿的方法、装置及相应的流式细胞分析仪 Download PDF

Info

Publication number
CN106255873A
CN106255873A CN201480075276.4A CN201480075276A CN106255873A CN 106255873 A CN106255873 A CN 106255873A CN 201480075276 A CN201480075276 A CN 201480075276A CN 106255873 A CN106255873 A CN 106255873A
Authority
CN
China
Prior art keywords
cell mass
compensation
value
benchmark
scatter diagram
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201480075276.4A
Other languages
English (en)
Other versions
CN106255873B (zh
Inventor
刘鹏昊
郭文恒
刘右林
金坚
闫华文
董丽芳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Mindray Bio Medical Electronics Co Ltd
Beijing Shen Mindray Medical Electronics Technology Research Institute Co Ltd
Original Assignee
Shenzhen Mindray Bio Medical Electronics Co Ltd
Beijing Shen Mindray Medical Electronics Technology Research Institute Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Mindray Bio Medical Electronics Co Ltd, Beijing Shen Mindray Medical Electronics Technology Research Institute Co Ltd filed Critical Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority to CN201910392236.7A priority Critical patent/CN110057744B/zh
Publication of CN106255873A publication Critical patent/CN106255873A/zh
Application granted granted Critical
Publication of CN106255873B publication Critical patent/CN106255873B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B45/00—ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B99/00—Subject matter not provided for in other groups of this subclass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Signal Processing (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Optics & Photonics (AREA)
  • Data Mining & Analysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

一种进行自动补偿的方法、相应的装置及流式细胞分析仪。该自动补偿的方法包括：根据需要进行补偿的散点图上各细胞群所处的位置，确定各细胞群中的基准细胞群以及参考细胞群，其中，基准细胞群为双阴性细胞群，参考细胞群为在补偿方向上与基准细胞群相邻的单阳性细胞群(S10)；根据基准细胞群的位置，通过逐步逼近的算法自动计算补偿值并以该补偿值更新散点图，使该散点图上参考细胞群与基准细胞群在被补偿方向上的位置差处于预定的范围(S16)。该方法可自动进行图形调节补偿，从而减少用户工作量，提升补偿的准确度。

Description

PCT国内申请，说明书已公开。

Claims (1)

  1. PCT国内申请，权利要求书已公开。
CN201480075276.4A 2014-04-10 2014-04-10 一种进行自动补偿的方法、装置及相应的流式细胞分析仪 Active CN106255873B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910392236.7A CN110057744B (zh) 2014-04-10 2014-04-10 一种进行自动补偿的方法、装置及相应的流式细胞分析仪

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2014/075109 WO2015154288A1 (zh) 2014-04-10 2014-04-10 一种进行自动补偿的方法、装置及相应的流式细胞分析仪

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201910392236.7A Division CN110057744B (zh) 2014-04-10 2014-04-10 一种进行自动补偿的方法、装置及相应的流式细胞分析仪

Publications (2)

Publication Number Publication Date
CN106255873A true CN106255873A (zh) 2016-12-21
CN106255873B CN106255873B (zh) 2019-06-04

Family

ID=54287126

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201910392236.7A Active CN110057744B (zh) 2014-04-10 2014-04-10 一种进行自动补偿的方法、装置及相应的流式细胞分析仪
CN201480075276.4A Active CN106255873B (zh) 2014-04-10 2014-04-10 一种进行自动补偿的方法、装置及相应的流式细胞分析仪

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN201910392236.7A Active CN110057744B (zh) 2014-04-10 2014-04-10 一种进行自动补偿的方法、装置及相应的流式细胞分析仪

Country Status (3)

Country Link
US (1) US9970857B2 (zh)
CN (2) CN110057744B (zh)
WO (1) WO2015154288A1 (zh)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018190340A1 (ja) * 2017-04-14 2018-10-18 国立研究開発法人国立がん研究センター エフェクター型制御性ｔ細胞の検出方法、エフェクター型制御性ｔ細胞の分析装置、エフェクター型制御性ｔ細胞の分析システム、および、プログラム
CN115176139A (zh) * 2020-01-31 2022-10-11 贝克顿·迪金森公司 用于调整训练门以适应流式细胞仪数据的方法和系统
CN112557284B (zh) * 2020-12-03 2023-05-30 桂林优利特医疗电子有限公司 一种进行自动调节cv质控的方法、装置及相应的流式细胞仪

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605805A (en) * 1993-02-09 1997-02-25 Becton, Dickinson And Company Automatic lineage assignment of acute leukemias by flow cytometry
US20070020697A1 (en) * 2005-07-25 2007-01-25 Hernani Cualing Virtual flow cytometry on immunostained tissue-tissue cytometer
CN101493400A (zh) * 2008-01-25 2009-07-29 深圳迈瑞生物医疗电子股份有限公司 一种基于形状特征的自动分类校正的方法
CN101853495A (zh) * 2010-06-03 2010-10-06 浙江工业大学 一种基于形态学的细胞分离方法
US20130183707A1 (en) * 2012-01-13 2013-07-18 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Stem cell bioinformatics

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030134305A1 (en) * 2001-08-16 2003-07-17 Dertinger Stephen D. Method for the enumeration of mammalian micronucleated erythrocyte populations with a single-laser flow cytometer
US6897954B2 (en) * 2002-12-20 2005-05-24 Becton, Dickinson And Company Instrument setup system for a fluorescence analyzer
KR101166180B1 (ko) * 2003-08-13 2012-07-18 루미넥스 코포레이션 유세포 분석기식 측정 시스템의 하나 이상의 파라미터의 제어 방법
EP1851545B1 (en) * 2005-02-25 2014-12-31 Dako Denmark A/S Cell counting
GB2428471A (en) * 2005-07-18 2007-01-31 Mathshop Ltd Flow cytometry
US7746713B2 (en) * 2007-09-12 2010-06-29 Massachusetts Institute Of Technology High density 45 nm SRAM using small-signal non-strobed regenerative sensing
CN101245368A (zh) * 2008-03-20 2008-08-20 重庆师范大学 应用流式细胞术中的散射光及自发荧光参数进行粘孢子虫分类的方法
US8154273B2 (en) * 2008-10-10 2012-04-10 Beckman Coulter, Inc. Detecting and handling coincidence in particle analysis
CN103180858B (zh) * 2011-08-25 2016-01-20 索尼公司 移动微实体流中的运动相关误差的补偿

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605805A (en) * 1993-02-09 1997-02-25 Becton, Dickinson And Company Automatic lineage assignment of acute leukemias by flow cytometry
US20070020697A1 (en) * 2005-07-25 2007-01-25 Hernani Cualing Virtual flow cytometry on immunostained tissue-tissue cytometer
CN101493400A (zh) * 2008-01-25 2009-07-29 深圳迈瑞生物医疗电子股份有限公司 一种基于形状特征的自动分类校正的方法
CN101853495A (zh) * 2010-06-03 2010-10-06 浙江工业大学 一种基于形态学的细胞分离方法
US20130183707A1 (en) * 2012-01-13 2013-07-18 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Stem cell bioinformatics

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
陈军浩 等: "流式细胞术Annexin V/PI检测细胞凋亡阳性界线值及补偿值的确定", 《现代检验医学杂志》 *
陶家平等: "流式细胞计多参数测量中的补偿问题", 《北京医科大学学报》 *

Also Published As

Publication number Publication date
WO2015154288A1 (zh) 2015-10-15
US9970857B2 (en) 2018-05-15
CN110057744B (zh) 2022-08-05
CN106255873B (zh) 2019-06-04
CN110057744A (zh) 2019-07-26
US20170097296A1 (en) 2017-04-06

Similar Documents

Publication Publication Date Title
US8532308B2 (en) Standing wave detection apparatus and method of controlling the same
CN106255873A (zh) 一种进行自动补偿的方法、装置及相应的流式细胞分析仪
JP6242291B2 (ja) 相分析装置、相分析方法、および表面分析装置
Ellwanger et al. Topologies of the (M+ 1) SSM with a singlino LSP at LEP2
JP6539829B1 (ja) 音声と非音声の度合いの検出方法
CN115421297B (zh) 光学系统最佳焦面位置的确定方法
CN107194902B (zh) 图像融合中自动确定引导滤波器参数的方法
CN114186596B (zh) 一种谱图峰的多窗口识别方法、装置以及电子设备
US8581186B2 (en) Charged particle beam apparatus
CN105093509B (zh) 显微镜的自动聚焦方法、系统及大便镜检显微镜
McCarthy et al. When does the non-variational nature of second-order Møller-Plesset energies manifest itself? All-electron correlation energies for open-shell atoms from K to Br
JP5400756B2 (ja) 荷電粒子線装置及び観察画像生成方法
EP2265965B1 (en) Relative noise of a measured signal
CN117278899A (zh) 一种蓝牙耳机的使用模式切换方法
CN108549928A (zh) 深度强化学习指导下基于连续移动的视觉跟踪方法及装置
CN108279809B (zh) 一种校准方法和装置
CN103767700B (zh) 一种心电波形电子测量的取样框调整方法和装置
JP2612353B2 (ja) エレベーター走行特性検査装置
US8165459B2 (en) Reproducing apparatus
CN114612518B (zh) 基于历史轨迹信息和细粒度匹配的孪生网络目标跟踪方法
CN106407550A (zh) 一种工业过程软测量建模方法
CN105991103A (zh) 一种音量控制方法及装置
CN108766455A (zh) 对混合信号进行降噪的方法和装置
DE102008003037B3 (de) Verfahren, Vorrichtung, computerlesbares Speichermedium und Computerprogramm-Element zum Ermitteln von physikalischen Eigenschaften atmosphärischer Partikel
Ogata et al. Inverse estimation of the vocal tract shape based on a vocal tract mapping interface

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant