CN106255763B - 核酸扩增 - Google Patents

核酸扩增 Download PDF

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Publication number
CN106255763B
CN106255763B CN201480073710.5A CN201480073710A CN106255763B CN 106255763 B CN106255763 B CN 106255763B CN 201480073710 A CN201480073710 A CN 201480073710A CN 106255763 B CN106255763 B CN 106255763B
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CN
China
Prior art keywords
primer
region
nucleic acid
nucleotide
template
Prior art date
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Active
Application number
CN201480073710.5A
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English (en)
Chinese (zh)
Other versions
CN106255763A (zh
Inventor
K·贝尔霍钦
J·李
P·帕特尔
A·理查德森
S·塔巴克曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Serranos Intellectual Property LLC
Original Assignee
Theranos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2014/030034 external-priority patent/WO2014145296A2/en
Application filed by Theranos Inc filed Critical Theranos Inc
Priority to CN202210640563.1A priority Critical patent/CN114807321A/zh
Publication of CN106255763A publication Critical patent/CN106255763A/zh
Application granted granted Critical
Publication of CN106255763B publication Critical patent/CN106255763B/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201480073710.5A 2013-11-22 2014-09-17 核酸扩增 Active CN106255763B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210640563.1A CN114807321A (zh) 2013-11-22 2014-09-17 核酸扩增

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201361908027P 2013-11-22 2013-11-22
US61/908,027 2013-11-22
PCT/US2014/030034 WO2014145296A2 (en) 2013-03-15 2014-03-15 Nuclei acid amplification
USPCT/US2014/030034 2014-03-15
US14/214,850 2014-03-15
US14/214,850 US9725760B2 (en) 2013-03-15 2014-03-15 Nucleic acid amplification
US201462001050P 2014-05-20 2014-05-20
US62/001,050 2014-05-20
PCT/US2014/056151 WO2015076919A1 (en) 2013-11-22 2014-09-17 Nucleic Acid Amplification

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202210640563.1A Division CN114807321A (zh) 2013-11-22 2014-09-17 核酸扩增

Publications (2)

Publication Number Publication Date
CN106255763A CN106255763A (zh) 2016-12-21
CN106255763B true CN106255763B (zh) 2022-06-24

Family

ID=53179994

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201480073710.5A Active CN106255763B (zh) 2013-11-22 2014-09-17 核酸扩增
CN202210640563.1A Pending CN114807321A (zh) 2013-11-22 2014-09-17 核酸扩增

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202210640563.1A Pending CN114807321A (zh) 2013-11-22 2014-09-17 核酸扩增

Country Status (12)

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EP (2) EP3957741A1 (enExample)
JP (3) JP2017500020A (enExample)
KR (1) KR20160088316A (enExample)
CN (2) CN106255763B (enExample)
AU (1) AU2014353462A1 (enExample)
BR (1) BR112016011692A2 (enExample)
CA (1) CA2927315A1 (enExample)
ES (1) ES2879823T3 (enExample)
HK (1) HK1232259A1 (enExample)
IL (1) IL245687A0 (enExample)
MX (1) MX370616B (enExample)
WO (1) WO2015076919A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2014233213A1 (en) 2013-03-15 2015-09-17 Theranos Ip Company, Llc Thermostable blunt-end ligase and methods of use
JP2017531424A (ja) 2014-09-17 2017-10-26 セラノス， インコーポレイテッドＴｈｅｒａｎｏｓ， Ｉｎｃ． ハイブリッド多元ステップ核酸増幅
US10822648B1 (en) 2016-07-29 2020-11-03 Labrador Diagnostics Llc Hybrid multi-step nucleic acid amplification
CN110819738A (zh) * 2019-11-20 2020-02-21 拱北海关技术中心 高致病性h7n9禽流感病毒的检测方法及其引物和探针
CN116438319A (zh) * 2020-10-21 2023-07-14 Illumina公司 包含多个插入物的测序模板以及用于提高测序通量的组合物和方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008032058A2 (en) * 2006-09-13 2008-03-20 Lingvitae As Method for sequencing a polynucleotide

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6251639B1 (en) * 1999-09-13 2001-06-26 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer
JP2004513617A (ja) * 2000-06-26 2004-05-13 ニューゲン　テクノロジーズ，　インコーポレイテッド 転写に基づく核酸増幅のための方法および組成物
JP4280519B2 (ja) * 2003-03-07 2009-06-17 アークレイ株式会社 Ｄｎａ増幅法およびそのためのキット
GB0504774D0 (en) * 2005-03-08 2005-04-13 Lingvitae As Method
WO2008070352A2 (en) * 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
GB0910302D0 (en) * 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
JP5299981B1 (ja) * 2011-09-08 2013-09-25 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008032058A2 (en) * 2006-09-13 2008-03-20 Lingvitae As Method for sequencing a polynucleotide

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Rapid detection of microbial DNA by a novel isothermal genome exponential amplification reaction (GEAR) assay;Jothikumar Prithiviraj等;《Biochemical and Biophysical Research Communications》;20120317;第420卷(第4期);第738-742页，摘要、图1 *

Also Published As

Publication number Publication date
MX370616B (es) 2019-12-17
EP3071713A1 (en) 2016-09-28
CA2927315A1 (en) 2015-05-28
JP2017500020A (ja) 2017-01-05
HK1232259A1 (zh) 2018-01-05
JP2019201670A (ja) 2019-11-28
WO2015076919A1 (en) 2015-05-28
ES2879823T3 (es) 2021-11-23
EP3957741A1 (en) 2022-02-23
JP2022172384A (ja) 2022-11-15
EP3071713B1 (en) 2021-05-12
CN114807321A (zh) 2022-07-29
EP3071713A4 (en) 2017-08-02
MX2016006602A (es) 2016-07-22
IL245687A0 (en) 2016-06-30
BR112016011692A2 (pt) 2017-09-26
AU2014353462A1 (en) 2016-05-05
CN106255763A (zh) 2016-12-21
KR20160088316A (ko) 2016-07-25

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