CN106255697B - 磷保护基团及其制备方法和用途 - Google Patents

磷保护基团及其制备方法和用途 Download PDF

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Publication number
CN106255697B
CN106255697B CN201580022581.1A CN201580022581A CN106255697B CN 106255697 B CN106255697 B CN 106255697B CN 201580022581 A CN201580022581 A CN 201580022581A CN 106255697 B CN106255697 B CN 106255697B
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China
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compound
group
protecting group
nucleic acid
nucleotides
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Chinese (zh)
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CN106255697A (zh
Inventor
D·J·德林杰
L·蒙弗雷戈拉
M·卡拉瑟斯
M·罗伊
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Agilent Technologies Inc
University of Colorado Colorado Springs
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Agilent Technologies Inc
University of Colorado Denver
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/173—Purine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/23—Heterocyclic radicals containing two or more heterocyclic rings condensed among themselves or condensed with a common carbocyclic ring system, not provided for in groups C07H19/14 - C07H19/22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201580022581.1A 2014-04-30 2015-04-30 磷保护基团及其制备方法和用途 Active CN106255697B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461986594P 2014-04-30 2014-04-30
US61/986,594 2014-04-30
PCT/US2015/028624 WO2015168461A2 (en) 2014-04-30 2015-04-30 Phosphorous protecting groups and methods of preparation and use thereof

Publications (2)

Publication Number Publication Date
CN106255697A CN106255697A (zh) 2016-12-21
CN106255697B true CN106255697B (zh) 2020-04-28

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US (3) US10196418B2 (enExample)
EP (2) EP3137478B1 (enExample)
JP (1) JP6730932B2 (enExample)
KR (1) KR102375480B1 (enExample)
CN (1) CN106255697B (enExample)
WO (1) WO2015168461A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015168461A2 (en) 2014-04-30 2015-11-05 Agilent Technologies, Inc. Phosphorous protecting groups and methods of preparation and use thereof
CN114685589A (zh) 2016-03-13 2022-07-01 波涛生命科学有限公司 用于亚磷酰胺和寡核苷酸合成的组合物和方法
WO2018098264A1 (en) 2016-11-23 2018-05-31 Wave Life Sciences Ltd. Compositions and methods for phosphoramidite and oligonucleotide synthesis
EP3609498A1 (en) 2017-05-01 2020-02-19 Theravance Biopharma R&D IP, LLC Methods of treatment using a jak inhibitor compound
CN113508103A (zh) 2019-02-28 2021-10-15 富士胶片株式会社 肽化合物的制造方法、保护基形成用试药及稠合多环芳香族烃化合物
CA3131774A1 (en) 2019-02-28 2020-09-03 Fujifilm Corporation Method for producing peptide compound, protecting group-forming reagent, and aromatic heterocyclic compound
EP4006045A4 (en) * 2019-08-29 2022-10-19 FUJIFILM Corporation METHOD FOR PRODUCTION OF A NUCLEIC ACID COMPOUND AND NUCLEIC ACID COMPOUND
EP3805242A1 (en) * 2019-10-07 2021-04-14 Sterna Biologicals GmbH & Co. KG Method for the production of a catalytically active dna molecule having improved activity and its use in a method of treating asthma
JP2024524214A (ja) 2021-06-25 2024-07-05 セラヴァンス バイオファーマ アール＆ディー アイピー， エルエルシー Ｊａｋ阻害剤としてのイミダゾロインダゾール化合物
CN113461743B (zh) * 2021-07-09 2023-03-17 中国石油大学(华东) 6-甲硫基乙基嘌呤-2’-脱氧核苷及其制备方法和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999040101A1 (en) * 1998-02-10 1999-08-12 Isis Pharmaceuticals, Inc. Synthetic process for the preparation of oligonucleotides, nucleoside synthons used therein, and oligonucleotides prepared thereby
US6274725B1 (en) * 1998-06-02 2001-08-14 Isis Pharmaceuticals, Inc. Activators for oligonucleotide synthesis
US20060178507A1 (en) * 2004-12-30 2006-08-10 Berry & Associates, Inc. Fluorous oligonucleotide reagents and affinity purification of oligonucleotides
US20130052690A1 (en) * 2011-08-22 2013-02-28 Samsung Electronics Co., Ltd. Pcr primer and method for reducing non-specific nucleic acid amplification using a photolabile protecting group

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
JPS6251695A (ja) * 1985-08-29 1987-03-06 Yuki Gosei Yakuhin Kogyo Kk ホスホロアミダイト類の合成法
NL8700724A (nl) * 1987-03-27 1988-10-17 Univ Eindhoven Tech Poly(deoxyribonucleotiden), farmaceutische samenstellingen, gebruik en bereiding van de poly(deoxyribonucleotiden).
US5955591A (en) * 1993-05-12 1999-09-21 Imbach; Jean-Louis Phosphotriester oligonucleotides, amidites and method of preparation
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
US6531590B1 (en) * 1998-04-24 2003-03-11 Isis Pharmaceuticals, Inc. Processes for the synthesis of oligonucleotide compounds
US6222030B1 (en) 1998-08-03 2001-04-24 Agilent Technologies, Inc. Solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
US7135565B2 (en) 2000-07-28 2006-11-14 Agilent Technologies, Inc. Synthesis of polynucleotides using combined oxidation/deprotection chemistry
CA2880869A1 (en) * 2012-08-20 2014-02-27 The Regents Of The University Of California Polynucleotides having bioreversible groups
WO2015168461A2 (en) 2014-04-30 2015-11-05 Agilent Technologies, Inc. Phosphorous protecting groups and methods of preparation and use thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999040101A1 (en) * 1998-02-10 1999-08-12 Isis Pharmaceuticals, Inc. Synthetic process for the preparation of oligonucleotides, nucleoside synthons used therein, and oligonucleotides prepared thereby
US6274725B1 (en) * 1998-06-02 2001-08-14 Isis Pharmaceuticals, Inc. Activators for oligonucleotide synthesis
US20060178507A1 (en) * 2004-12-30 2006-08-10 Berry & Associates, Inc. Fluorous oligonucleotide reagents and affinity purification of oligonucleotides
US20130052690A1 (en) * 2011-08-22 2013-02-28 Samsung Electronics Co., Ltd. Pcr primer and method for reducing non-specific nucleic acid amplification using a photolabile protecting group

Also Published As

Publication number Publication date
US20190194242A1 (en) 2019-06-27
EP3137478B1 (en) 2021-09-22
JP6730932B2 (ja) 2020-07-29
KR20160147973A (ko) 2016-12-23
CN106255697A (zh) 2016-12-21
US11584773B2 (en) 2023-02-21
WO2015168461A3 (en) 2015-12-23
US20220002334A1 (en) 2022-01-06
US10196418B2 (en) 2019-02-05
US20150315227A1 (en) 2015-11-05
JP2017518966A (ja) 2017-07-13
KR102375480B1 (ko) 2022-03-16
WO2015168461A2 (en) 2015-11-05
US11104699B2 (en) 2021-08-31
EP3137478A2 (en) 2017-03-08
EP3137478A4 (en) 2018-03-07
EP3943500A1 (en) 2022-01-26

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