CN106248626A - A kind of detection method of soil humic acid content - Google Patents
A kind of detection method of soil humic acid content Download PDFInfo
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- CN106248626A CN106248626A CN201610883148.3A CN201610883148A CN106248626A CN 106248626 A CN106248626 A CN 106248626A CN 201610883148 A CN201610883148 A CN 201610883148A CN 106248626 A CN106248626 A CN 106248626A
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- soil
- humic acid
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- acid content
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
Abstract
The invention discloses the detection method of a kind of soil humic acid content, comprise the following steps: gather the required pedotheque measuring content of humic acid；By the pedotheque removal of impurity, air-dried, grinding, under the conditions of 45 55 DEG C, finally it is dried 2h, obtains soil to be measured；Measure the soil to be measured reflectance at 350 2500nm wave bands, obtain the reflectivity data of 350 2500nm wave bands；After the reflectivity data of 350 2500nm wave bands is carried out multiplicative scatter correction, use wavelet de-noising, then be smoothed；Extract the soil to be measured reflectivity data at specific wavelength；The reflectivity data extracted is substituted into soil humic acid content detection model, calculates the content of humic acid of this pedotheque.The detection method of soil humic acid content of the present invention, achieve the quick and precisely detection of content of humic acid in soil, need not prepare any chemical reagent, avoid the chemistry tail liquid emissions pollution to environment and the injury to human body during mensuration, also greatly simplify operating procedure simultaneously, shorten the detection time.
Description
Technical field
The invention belongs to soil nutrient detection technique field, be specifically related to the detection method of a kind of soil humic acid content.
Background technology
Humic acid is animals and plants remains through the decomposition of microorganism and conversion and geochemical a series of process and shape
The type organic become and accumulate, its molecular surface has more oxygen-containing functional group, has faintly acid, colloidality, absorption
Property, complexing and the oxidation feature such as originality, soil fertilizer, feeder capability can be improved, and form crumb structure, thus improve soil
The constructional aspect of earth, makes soil fertility be improved.Poisonous organic or inorganic chemical materials many in environment are deposited by humic acid
Very important effect, heavy metal ion and the transmission of organic pollution is being played with transport, the cyclic process of nutrient substance
Have a great impact with conversion.Humic acid contains various nutrient elements necessary to growth and development of plants, obtains the corruption in soil
Grow acid content data, be soil fertility diagnosis, the digit basis of soil rational application of fertilizer necessity, be also Land planning and utilization
Important evidence.
The detection method of existing soil humic acid content needs to prepare chemical reagent, chemistry tail liquid emissions during mensuration
Pollution to environment and the injury to human body, and there is complex operation step, the defect of detection time length.Therefore, it is necessary to
Develop the detection method of pollution-free, easy and simple to handle, the most accurate a kind of soil humic acid content.
Summary of the invention
It is an object of the invention to provide the detection method of a kind of soil humic acid content, solve existing detection method and measure
During the chemistry tail liquid emissions pollution to environment and the injury to human body, and there is complex operation step, the detection time is long
Problem.
The detection method of a kind of soil humic acid content that the present invention provides, comprises the following steps:
Step 1, gathers the required pedotheque measuring content of humic acid；
Step 2, by the pedotheque removal of impurity, air-dried, grinding, is finally dried 2h under the conditions of 45-55 DEG C, obtains soil to be measured
Earth；
Step 3, measures the soil to be measured reflectance at 350-2500nm wave band, obtains the reflectance of 350-2500nm wave band
Data；
Step 4, after the reflectivity data of 350-2500nm wave band is carried out multiplicative scatter correction, uses wavelet de-noising, then
It is smoothed；
Step 5, extract soil to be measured 500nm, 556nm, 559nm, 560nm, 561nm, 562nm, 1341nm,
The reflectivity data of 1342nm, 1343nm, 1413nm, 1424nm, 1425nm, 1426nm, 2093nm wavelength；
Step 6, the reflectivity data substitution soil humic acid content detection model that will extract:
Y=28.98904x1-10.24487x2-16.95119x3-15.52339x4-14.64154x5-15.1408x6-
59.47598x7-57.51946x8-56.13704x9+72.16309x10+88.56385x11+85.33824x12+83.2752x13-
110.1966x14+ 1.77989, calculate the content of humic acid of this pedotheque；
Wherein, Y is content of humic acid, and unit is g/kg；x1、x2、x3、x4、x5、x6、x7、x8、x9、x10、x11、x12、x13、
x14Be respectively soil to be measured 500nm, 556nm, 559nm, 560nm, 561nm, 562nm, 1341nm, 1342nm, 1343nm,
The reflectance of 1413nm, 1424nm, 1425nm, 1426nm, 2093nm wavelength.
Preferably, in step 2, by pedotheque in room-dry, and after grinding, via diameter is the sieve of 2mm.
Preferably, in step 3, the determining instrument of described spectral reflectivity is the QualitySpec that ASD company of the U.S. produces
Trek Handheld spectrometer, its spectral resolution 350-700nm be 3nm, 701-1400nm be that 9.8nm, 1401-2500nm are
8.1nm。
Preferably, in step 3, soil to be measured is laid in the pitch-dark culture dish of a diameter of 5cm, during mensuration, keeps
Indoor environment temperature is constant, control well QualitySpec Trek Handheld spectrometer probe with soil surface to be measured away from
From, make probe internally provided light source not light leak.
Preferably, after soil to be measured is laid in the culture dish of a diameter of 5cm, the thickness of soil to be measured is 10-15mm.
Preferably, in step 4, the method that described smoothing processing uses is Savitzky-Golay Smoothing, smooth
Window is 7.
Preferably, in step 5,500nm, 556nm, 559nm, 560nm, 561nm, 562nm, 1341nm, 1342nm,
The reflectivity data of 1343nm, 1413nm, 1424nm, 1425nm, 1426nm, 2093nm wavelength, the most same soil to be measured
It is repeated 4 times the meansigma methods of the reflectivity data of mensuration.
The detection method of soil humic acid content of the present invention, it is achieved that the quick and precisely detection of content of humic acid in soil,
Need not prepare any chemical reagent, it is to avoid the chemistry tail liquid emissions pollution to environment and the wound to human body during measuring
Evil, also greatly simplify operating procedure simultaneously, shortens the detection time.Soil humic acid data determination in agricultural production can be met
Demand, it is provided that the means of quick obtaining dose foundation, be suitable for popularization and application.
Accompanying drawing explanation
Fig. 1 soil humic acid measured value and detected value scatterplot.
Detailed description of the invention
Below in conjunction with the accompanying drawings and specific embodiment, the detailed description of the invention of invention is described in detail, it is to be understood that
Protection scope of the present invention is not limited by detailed description of the invention.
Embodiment 1
Step 1, gathers the required pedotheque measuring content of humic acid, and pedotheque depth selection is 20-40cm, due to
In sample area, the content of humic acid of diverse location soil is different, in order to draw the corruption in this regional soil more accurately
Growing acid content, in order to verify feasibility and the repeatability of the method, we acquire 100 pedotheques altogether and are measured.
Step 2, by the pedotheque removal of impurity, disposed within air-dried, with mortar grinder, then via diameter is the sieve of 2mm,
Last dry 2h under the conditions of 50 DEG C, obtains soil to be measured.
Described impurity is stone, macroscopic root system of plant and dry branches and fallen leaves.
It should be noted that the arbitrary temperature between 45-55 DEG C is used equally to dry soil sample, and can reach same
Effect, therefore the present embodiment is only as a example by 50 DEG C, and the step of the present invention is described.
Step 3, is laid in soil to be measured in the pitch-dark culture dish of a diameter of 5cm, and soil to be measured is laid in diameter
After the culture dish of 5cm, the thickness of soil to be measured is 15mm, during mensuration, keeps indoor environment temperature constant, controls well
The probe of QualitySpec Trek Handheld spectrometer and the distance of soil surface to be measured, make probe internally provided light source not light leak,
Then the QualitySpec Trek Handheld spectrometer produced with ASD company of the U.S. measures soil to be measured at 350-2500nm ripple
The reflectance of section, obtains the reflectivity data of 350-2500nm wave band.
As long as may be used for measuring reflectance it should be noted that soil is paved with flat board, it is preferred that soil to be measured
Thickness is 10-15mm, and above-mentioned condition all can reach same effect, therefore the present embodiment is only as a example by 15mm, illustrates the present invention's
Step.
It should be noted that in the QualitySpec Trek Handheld spectrometer of ASD company production, spectral resolution exists
350-700nm be 3nm, 701-1400nm be 9.8nm, 1401-2500nm be 8.1nm.
Step 4, after the reflectivity data of 350-2500nm wave band is carried out multiplicative scatter correction, uses wavelet de-noising, then
Being smoothed, the method that smoothing processing uses is Savitzky-Golay Smoothing, and smooth window is 7；
Step 5, extract soil to be measured 500nm, 556nm, 559nm, 560nm, 561nm, 562nm, 1341nm,
The reflectivity data of 1342nm, 1343nm, 1413nm, 1424nm, 1425nm, 1426nm, 2093nm wavelength；
Step 6, the reflectivity data substitution soil humic acid content detection model that will extract:
Y=28.98904x1-10.24487x2-16.95119x3-15.52339x4-14.64154x5-15.1408x6-
59.47598x7-57.51946x8-56.13704x9+72.16309x10+88.56385x11+85.33824x12+83.2752x13-
110.1966x14+ 1.77989, calculate the content of humic acid of this pedotheque；
Wherein, Y is content of humic acid, and unit is g/kg；x1、x2、x3、x4、x5、x6、x7、x8、x9、x10、x11、x12、x13、
x14Be respectively soil to be measured 500nm, 556nm, 559nm, 560nm, 561nm, 562nm, 1341nm, 1342nm, 1343nm,
The reflectance of 1413nm, 1424nm, 1425nm, 1426nm, 2093nm wavelength.
It should be noted that for the content of humic acid detecting each pedotheque more accurately, we are to same soil
Earth sample is repeated 4 times mensuration, obtains the reflectivity data of 4 groups of 350-2500nm wave bands, and to often organizing 350-2500nm wave band
After reflectivity data carries out multiplicative scatter correction, use wavelet de-noising, then be smoothed；Extract the same of 4 mensuration respectively
One pedotheque 500nm, 556nm, 559nm, 560nm, 561nm, 562nm, 1341nm, 1342nm, 1343nm, 1413nm,
The reflectivity data of 1424nm, 1425nm, 1426nm, 2093nm wavelength, takes the meansigma methods of corresponding wavelength respectively, brings soil into rotten
Grow acid content detection model, i.e. obtain the content of humic acid of a pedotheque.
Table 1 is that the actual value of 100 the pedotheque content of humic acid utilizing indoor chemical assay to obtain is real with utilization
Executing the statistical data of the detected value that the method for example 1 obtains, as known from Table 1,100 pedotheque actual values are average with detected value
Value, maximum, minima are the most close；Fig. 1 is the fitting degree of pedotheque content of humic acid detected value and actual value.Very
The coefficient of determination (R between real-valued and detected value2) reaching 0.79, root-mean-square error (RMSE) only has 0.48g/kg, average absolute
Error (MAE) only has 0.37g/kg, and standard deviation reaches 2.20 with root-mean-square error ratio (RPD), according to RPD evaluation criterion, RPD >
2.0, illustrate that model has the ability of high-precision forecast.This result shows, the method for the present invention can detect soil accurately and rapidly
Earth sample content of humic acid.
Table 1 soil humic acid content detection result
Although preferred embodiments of the present invention have been described, but those skilled in the art once know basic creation
Property concept, then can make other change and amendment to these embodiments.So, claims are intended to be construed to include excellent
Select embodiment and fall into all changes and the amendment of the scope of the invention.
Obviously, those skilled in the art can carry out various change and the modification essence without deviating from the present invention to the present invention
God and scope.So, if these amendments of the present invention and modification belong to the scope of the claims in the present invention and equivalent technologies thereof
Within, then the present invention is also intended to comprise these change and modification.
Claims (7)
1. the detection method of a soil humic acid content, it is characterised in that comprise the following steps:
Step 1, gathers the required pedotheque measuring content of humic acid；
Step 2, by the pedotheque removal of impurity, air-dried, grinding, is finally dried 2h under the conditions of 45-55 DEG C, obtains soil to be measured；
Step 3, measures the soil to be measured reflectance at 350-2500nm wave band, obtains the reflectance number of 350-2500nm wave band
According to；
Step 4, after the reflectivity data of 350-2500nm wave band is carried out multiplicative scatter correction, uses wavelet de-noising, then carries out
Smoothing processing；
Step 5, extract soil to be measured 500nm, 556nm, 559nm, 560nm, 561nm, 562nm, 1341nm, 1342nm,
The reflectivity data of 1343nm, 1413nm, 1424nm, 1425nm, 1426nm, 2093nm wavelength；
Step 6, the reflectivity data substitution soil humic acid content detection model that will extract:
Y=28.98904x1-10.24487x2-16.95119x3-15.52339x4-14.64154x5-15.1408x6-
59.47598x7-57.51946x8-56.13704x9+72.16309x10+88.56385x11+85.33824x12+83.2752x13-
110.1966x14+ 1.77989, calculate the content of humic acid of this pedotheque；
Wherein, Y is content of humic acid, and unit is g/kg；x1、x2、x3、x4、x5、x6、x7、x8、x9、x10、x11、x12、x13、x14Respectively
For soil to be measured 500nm, 556nm, 559nm, 560nm, 561nm, 562nm, 1341nm, 1342nm, 1343nm, 1413nm,
The reflectance of 1424nm, 1425nm, 1426nm, 2093nm wavelength.
The detection method of soil humic acid content the most according to claim 1, it is characterised in that in step 2, by soil-like
Product are in room-dry, and after grinding, via diameter is the sieve of 2mm.
The detection method of soil humic acid content the most according to claim 1, it is characterised in that in step 3, described spectrum
The determining instrument of reflectance is the QualitySpec Trek Handheld spectrometer that ASD company of the U.S. produces, its spectral resolution
350-700nm be 3nm, 701-1400nm be 9.8nm, 1401-2500nm be 8.1nm.
The detection method of soil humic acid content the most according to claim 3, it is characterised in that in step 3, by soil to be measured
Earth is laid in the pitch-dark culture dish of a diameter of 5cm, during mensuration, keeps indoor environment temperature constant, controls well
The probe of QualitySpec Trek Handheld spectrometer and the distance of soil surface to be measured, make probe internally provided light source not light leak.
The detection method of soil humic acid content the most according to claim 4, it is characterised in that soil to be measured is laid in
After the culture dish of a diameter of 5cm, the thickness of soil to be measured is 10-15mm.
The detection method of soil humic acid content the most according to claim 1, it is characterised in that in step 4, described smooth
Processing the method used is Savitzky-Golay Smoothing, and smooth window is 7.
The detection method of soil humic acid content the most according to claim 1, it is characterised in that in step 5,500nm,
556nm、559nm、560nm、561nm、562nm、1341nm、1342nm、1343nm、1413nm、1424nm、1425nm、
The reflectivity data of 1426nm, 2093nm wavelength, the most same soil to be measured is repeated 4 times the average of the reflectivity data of mensuration
Value.
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Citations (3)
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|---|---|---|---|---|
| CN101769868A (en) * | 2008-12-29 | 2010-07-07 | 中国科学院沈阳应用生态研究所 | Soil moisture reflection spectrum characteristic analysis method |
| CN102830071A (en) * | 2012-08-13 | 2012-12-19 | 浙江大学 | Detection apparatus and method for total phosphorus content in soil |
| CN103048276A (en) * | 2012-12-14 | 2013-04-17 | 北京农业信息技术研究中心 | Spectral index constructing method for detecting carbon nitrogen ratios of canopy leaves of crops |
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2016
- 2016-10-09 CN CN201610883148.3A patent/CN106248626A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101769868A (en) * | 2008-12-29 | 2010-07-07 | 中国科学院沈阳应用生态研究所 | Soil moisture reflection spectrum characteristic analysis method |
| CN102830071A (en) * | 2012-08-13 | 2012-12-19 | 浙江大学 | Detection apparatus and method for total phosphorus content in soil |
| CN103048276A (en) * | 2012-12-14 | 2013-04-17 | 北京农业信息技术研究中心 | Spectral index constructing method for detecting carbon nitrogen ratios of canopy leaves of crops |
Non-Patent Citations (3)
| Title |
|---|
| 丁海泉 等: "土壤有机质近红外光谱分析组合波长的优选", 《光学精密工程》 * |
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Application publication date: 20161221 |