CN106244448A - A kind of bacterium culture systems - Google Patents
A kind of bacterium culture systems Download PDFInfo
- Publication number
- CN106244448A CN106244448A CN201610627195.1A CN201610627195A CN106244448A CN 106244448 A CN106244448 A CN 106244448A CN 201610627195 A CN201610627195 A CN 201610627195A CN 106244448 A CN106244448 A CN 106244448A
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- Prior art keywords
- bacterium
- air
- filter
- culture systems
- degrees celsius
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
Abstract
The invention discloses a kind of bacterium culture systems, including: culturing rack, culturing rack is used for placing bacterium bag；Temperature control inner blower, temperature control inner blower is placed on culturing rack top；Air exhausting device, for providing oxygen during mycelial growth, air exhausting device includes that supply fan is identical with the wind direction of exhaust fan and temperature control inner blower with exhaust fan and supply fan；Thermostat, for controlling the bacterium temperature in environment；And purifier, for sending out the air filtration sterilization of collarium border.The bacterium culture systems structure of sending out of the present invention arranges rationally, it is ensured that sends out the oxygen needed for bacterium and temperature, improves and send out the efficiency that bacterium is cultivated；A collarium border has been carried out filter-sterilized process, it is ensured that the survival rate of edible fungi cultivation and quality simultaneously.
Description
Technical field
The application relates to edible fungi and cultivates field, is specifically related to a kind of bacterium culture systems.
Background technology
The solid spawn production cycle is long, and cultivating bag sterilizing efficiency is low, becomes the bottleneck of Edible Fungi development.Cured material bag-cultured
Pollute the most important reason that inoculation link is not carried out being to cause bacterium bag to pollute by sterile working's requirement.When in production, mushroom agriculture is in order to rob
Between carry out Open-Architecture Controller operation more i.e. to complete inoculation in mushroom shed be unscientific.In the environment of low temperature, miscellaneous bacteria are lacked so
The possible pollution rate of operation is the highest but once finds high pollution, has resulted in loss.Living contaminants bacterium bag is processed points for attention
Have: one is must be by the bacterium bag polluted and untainted bacterium bag separate management；Two is that the lighter bacterium bag of pollution can be used office
The method of portion's injection treatment is remedied.Will be appreciated that this is that a polluter once bacterium bag ruptures that to discharge countless miscellaneous bacteria spore tight
Heavily harm Edible Fungi environment.Pollute the most handy inoculating hood or transfer room complete inoculation, in traditional handicraft so wanting to reduce
Just carry out fumigation strict aseptic technique before using, but the surrounding air after sterilizing and in seeded process is not entered by it
Row purifies.
Cultivation base stock for edible fungi needs autoclave sterilization, under the planting material after sterilizing just can be carried out after cooling down
The operation of one step.If the method using natural cooling, not only length cool time has a strong impact on efficiency, and is likely to result in cultivation
Becoming sour of material.During whole cooling, the temperature of bacterium bag is by high step-down, and overall volume also has reducing slightly, and i.e. one
The individual process expanded with heat and contract with cold, this contraction process will result in extraneous air and sucks in bag from resistance bacterium hole, for ensureing inoculation environment
Cleaning, reduces pollution risk, and whole cleaning system is it is necessary that clean to the complete environment during this of inoculation after sterilizing
Only.
In sum, edible fungi is sent out fungus strain system and be there is temperature and oxygen supply is the most constant, sends out collarium border air cleaning journey
Degree not, causes sending out the problem that bacterium efficiency success rate reduces, needs the temperature to whole bacterium culture systems and oxygen to have
Effect controls, and purifies air, to improve the clean level sending out collarium border, thus improves efficiency and the success sending out bacterium
Rate.
Summary of the invention
The present invention provides a kind of and sends out bacterium culture systems, it is adaptable to edible fungi is cultivated, and solves temperature and oxygen supply is inadequate
Constant, send out collarium border air cleaning degree inadequate, cause sending out the problem that bacterium efficiency success rate reduces.
In order to solve the problems referred to above, this application discloses a kind of bacterium culture systems, including: culturing rack, culturing rack is used for
Place bacterium bag；Temperature control inner blower, temperature control inner blower is placed on culturing rack top；Air exhausting device, is placed on the corner sending out bacterium room,
For providing oxygen, air exhausting device to include supply fan and exhaust fan and supply fan and exhaust fan and temperature during mycelial growth
The wind direction of control inner blower is identical；Thermostat, is placed on the sidewall sending out bacterium room, for controlling the bacterium temperature in environment；And
Purifier, is placed on the roof sending out bacterium room, for sending out the air filtration sterilization of collarium border.
Further, described supply fan and the above-mentioned exhaust fan quantity of air exhausting device are equal, distribution and mutually correspondence.
Further, supply fan lower edge is higher than the upper limb of temperature control inner blower.
Further, the minimum altitude of exhaust fan lower edge is above the ground level.
Further, a bacterium temperature is controlled between 21 degrees Celsius to 25 degrees Celsius by thermostat.
Further, air exhausting device arranges two stages, and first stage air draft sooner or later, each 15 minutes, second stage set
Determine every air draft in 3 hours once, one time 30 minutes.
Further, send out three phases before, during and after bacterium temperature is set to, be respectively as follows: early stage 22 degrees Celsius Celsius to 24
Degree, mid-term 21 degrees Celsius to 23 degrees Celsius, the later stage 23 degrees Celsius to 25 degrees Celsius.
Further, purifier includes: filter, and filter sends into clean area after being filtered by extraneous air；Return air
Device, corresponding filter, extraneous air is formed backflow and delivers to filter and filter by Wind return device described in corresponding described filter；With
And disinfector, corresponding described filter, filter filtered air is carried out disinfection by disinfector.
Further, disinfector includes ozone sterilization assembly and/or uviol lamp.
Further, filter comprises primary air filter plant, middle rank filter plant and the senior filtration being sequentially connected
Equipment.
Compared with prior art, the application can obtain and include techniques below effect:
One, present invention employs laminar culturing rack, improve space availability ratio, prevent bacterium bag and push corrupt showing
As；
Two, present invention employs temperature control inner blower and air exhausting device, it is ensured that the oxygen of culture environment and heat radiation, be conducive to
The growing of edible fungi；
Three, present invention employs thermostat, it is ensured that a bacterium temperature of culture environment, improve the quality of edible fungi；
Four, present invention employs purifier, it is ensured that the clean level of culture environment, reduce pollution risk, improve
Send out the success rate of bacterium.
Accompanying drawing explanation
Accompanying drawing described herein is used for providing further understanding of the present application, constitutes the part of the application, this Shen
Schematic description and description please is used for explaining the application, is not intended that the improper restriction to the application.In the accompanying drawings:
Fig. 1 is the structural representation that the present invention sends out bacterium culture systems；
Fig. 2 is the structural representation that the present invention sends out the purifier of bacterium culture systems；
Detailed description of the invention
Describe presently filed embodiment in detail below in conjunction with drawings and Examples, thereby how the application is applied
Technological means solves technical problem and reaches the process that realizes of technology effect and can fully understand and implement according to this.
Also, it should be noted term " includes ", " comprising " or its other variant any are intended to nonexcludability
Comprise, so that include that the process of a series of key element, method, commodity or system not only include those key elements, but also wrap
Include other key element being not expressly set out, or also include want intrinsic for this process, method, commodity or system
Element.In the case of there is no more restriction, statement " including ... " key element limited, it is not excluded that including described wanting
Process, method, commodity or the system of element there is also other identical element.
The bacterium culture systems of sending out of the present invention includes: culturing rack 11, culturing rack is used for placing bacterium bag；Temperature control inner blower 12, temperature
Control inner blower is placed on culturing rack top；Air exhausting device 13, is placed on the corner sending out bacterium room, for during mycelial growth
Thering is provided oxygen, air exhausting device includes supply fan 131 and exhaust fan 132 and supply fan and exhaust fan and the wind direction of temperature control inner blower
Identical；Thermostat 14, is placed on the sidewall sending out bacterium room, for controlling the bacterium temperature in environment；And purifier 15, put
Put at the roof sending out bacterium room, for sending out the air filtration sterilization of collarium border.
Specifically, seeing accompanying drawing 1, the present invention uses tier rack type to cultivate, bacterium bag in being placed on small basket whole basket be placed in culturing rack 11
On, every layer of shelf has certain intervals, such as 45 centimetres, to ensure the patency of air circulation in bacterium bag incubation.Meanwhile, may be used
Cultivate in batches according to producing needs.It is pointed out that culturing rack arranges the temperature control inner blower 12 being suitable for the present invention,
With thermostat 14, air exhausting device 13 arranges that coordination is corresponding, this is preferably to send out bacterium for the acquirement that cooperates to cultivate bar
Part, the such as highest distance position of culturing rack 11 are arranged within the maximum range of air exhausting device 13 to ensure air circulation.This is this
The selection that skilled person can be carried out according to common knowledge.The most do not do too much restriction.
The fungus strain system of sending out of the present invention is provided with temperature control inner blower 12 and air exhausting device 13.Temperature control inner blower 12 is placed on cultivation
The top of frame 11, wind direction by door in room side-blown, can promote to cultivate the circulation of upper end air.Air exhausting device 13 is placed on to be sent out
One corner of bacterium room, including supply fan 131 and exhaust fan 132, they can be one or more, and quantity is identical, such as,
As one embodiment of the present of invention, their quantity can be 4, sets according to sending out collarium border.Supply fan 131 lower edge is set
Higher than the upper limb of temperature control inner blower, such as higher than temperature control inner blower 10 centimetres.The minimum altitude of exhaust fan lower edge is set above Ground
Face, such as exhaust fan are liftoff 20 centimetres.Further, supply fan and exhaust fan are uniformly arranged according to the landform sending out bacterium place, have one
Fixed interval, and mutually corresponding, supply fan 131 is identical with the wind direction of exhaust fan 132 and temperature control inner blower 12 simultaneously, it is ensured that cultivate
The circulation of frame both under air.As one embodiment of the present of invention, air exhausting device arranges two stages, first stage row sooner or later
Wind, each 15 minutes, second stage set every air draft in 3 hours once, one time 30 minutes.The stage of air exhausting device and air draft
Interval with send out bacterium temperature to associating.One of ordinary skill in the art can carry out the setting of air draft pattern according to a bacterium situation.
Temperature control inner blower and air exhausting device make whole bacterium system air circulation smooth and easy, provide oxygen for mycelial growth.
The present invention sends out the thermostat 14 of fungus strain system and is sent out a bacterium temperature taken the photograph to 25 at 21 degrees Celsius by temperature control device control, holding
Between family name's degree.As one embodiment of the present of invention, thermostat 14 can be placed in the sidewall sending out bacterium room, sends out bacterium temperature and is set to
Before, during and after three phases, be respectively as follows: early stage 22 degrees Celsius to 24 degrees Celsius, mid-term 21 degrees Celsius to 23 degrees Celsius, later stage 23
Degree Celsius to 25 degrees Celsius.It will be appreciated by those skilled in the art that the temperature of three phases sets according to sending out bacterium situation,
Within a suitable bacterium each falls within the scope of the present invention.
Seeing accompanying drawing 2, the present invention sends out bacterium culture apparatus and also includes purifier 15, for disappearing a collarium border air filtration
Poison.Purifier 15 is placed on the roof sending out bacterium room, including filter 151, sends into clean area after being filtered by extraneous air；Return
Wind device 152, extraneous air is formed backflow and delivers to filter and filter by corresponding described filter；And disinfector 153, to mistake
Filter filtered air carries out disinfection.Sending out in bacterium culture systems in the present invention, filter includes the primary air being sequentially connected
Filter plant 1511, middle rank filter plant 1512 and senior filter plant 1513, this is the division carried out according to filtration grade, protects
Demonstrate,prove a clean level for bacterium surrounding air.It should be noted that filter 151 is periodically replaced according to detecting appraisal, with
Ensure stability and the effectiveness of its work.As one embodiment of the present of invention, disinfector 153 includes ozone sterilization assembly
And/or uviol lamp.During duty, sterilizing installation is closed, elementary, middle and high three set air filter unit and Wind return devices
152 open, and extraneous air sends into clean area after three road defecators filter, and partial air is formed by Wind return device 152
Backflow is sent to filter again, and another part air is expelled directly out outdoor, makes clean area and outdoor formation malleation, outside not with prevention
Purified air enters indoor.It will be appreciated by those skilled in the art that sending out bacterium culture systems is in sterile purification space
Use.
Described above illustrate and describes some preferred embodiments of the present invention, but as previously mentioned, it should be understood that the present invention
Be not limited to form disclosed herein, be not to be taken as the eliminating to other embodiments, and can be used for other combinations various,
Amendment and environment, and can be in invention contemplated scope described herein, by above-mentioned teaching or the technology of association area or knowledge
It is modified.And the change that those skilled in the art are carried out and change are without departing from the spirit and scope of the present invention, the most all should be at this
In the protection domain of bright claims.
Claims (10)
1. send out bacterium culture systems for one kind, it is characterised in that including:
Culturing rack, described culturing rack is used for placing bacterium bag；
Temperature control inner blower, described temperature control inner blower is placed on described culturing rack top；
Air exhausting device, is placed on the corner sending out bacterium room, and described air exhausting device includes supply fan and exhaust fan and described supply fan
Identical with the wind direction of described exhaust fan and described temperature control inner blower；
Thermostat, is placed on and sends out bacterium room sidewall, for controlling the bacterium temperature in environment；And
Purifier, is placed on the roof sending out bacterium room, for sending out the air filtration sterilization of collarium border.
The most according to claim 1 bacterium culture systems, it is characterised in that the described supply fan of described air exhausting device and upper
State exhaust fan quantity equal, distribution and mutually correspondence.
The most according to claim 1 bacterium culture systems, it is characterised in that described supply fan lower edge is higher than in described temperature control
The upper limb of blower fan.
The most according to claim 1 bacterium culture systems, it is characterised in that the maximum height of described exhaust fan lower edge is higher than
Away from ground.
The most according to claim 1 bacterium culture systems, it is characterised in that described thermostat will be sent out bacterium temperature and control
Between 21 degrees Celsius to 25 degrees Celsius.
The most according to claim 1 bacterium culture systems, it is characterised in that described air exhausting device arranges two stages, the
The air draft sooner or later of one stage, each 15 minutes, second stage set every air draft in 3 hours once, one time 30 minutes.
Send out bacterium culture systems the most according to claim 1 or 5, it is characterised in that before described bacterium temperature is set to, in,
Rear three phases, is respectively as follows: early stage 22 degrees Celsius to 24 degrees Celsius, mid-term 21 degrees Celsius to 23 degrees Celsius, later stage 23 degrees Celsius
To 25 degrees Celsius.
The most according to claim 1 bacterium culture systems, other are characterised by, described purifier includes:
Filter, described filter sends into described clean area after being filtered by extraneous air；
Wind return device, corresponding described filter, the formation backflow of described extraneous air is delivered to described filter and is carried out by described Wind return device
Filter；And
Disinfector, corresponding described filter, described filter filtered air is carried out disinfection by described disinfector.
Purifier the most according to claim 8, other are characterised by, described disinfector include ozone sterilization assembly and/
Or uviol lamp.
Purifier the most according to claim 8, other are characterised by, described filter comprises the primary being sequentially connected
Air filter unit, middle rank filter plant and senior filter plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610627195.1A CN106244448B (en) | 2016-08-03 | 2016-08-03 | A kind of bacterium germination culture systems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610627195.1A CN106244448B (en) | 2016-08-03 | 2016-08-03 | A kind of bacterium germination culture systems |
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| Publication Number | Publication Date |
|---|---|
| CN106244448A true CN106244448A (en) | 2016-12-21 |
| CN106244448B CN106244448B (en) | 2019-04-09 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610627195.1A Active CN106244448B (en) | 2016-08-03 | 2016-08-03 | A kind of bacterium germination culture systems |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182550A (en) * | 2017-06-25 | 2017-09-22 | 江苏福利达农业科技有限公司 | A kind of gas circulating device for cultivating edible mushrooms |
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| US20120083030A1 (en) * | 2010-09-30 | 2012-04-05 | Sanyo Electric Co., Ltd. | Incubator |
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| CN205232874U (en) * | 2015-10-30 | 2016-05-18 | 重庆市洪欣食用菌有限公司 | Domestic fungus disinfects and filters culture tank |
| CN205266362U (en) * | 2015-12-14 | 2016-06-01 | 河南珍福农业科技有限公司 | Edible fungus incubator |
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|---|---|---|---|---|
| CN2399026Y (en) * | 1999-12-24 | 2000-10-04 | 石磊 | Multifunctional aseptic purifying storage case |
| US20120083030A1 (en) * | 2010-09-30 | 2012-04-05 | Sanyo Electric Co., Ltd. | Incubator |
| US20120164721A1 (en) * | 2010-12-22 | 2012-06-28 | Hitachi, Ltd. | Cell culture apparatus |
| CN204307646U (en) * | 2014-12-18 | 2015-05-06 | 齐齐哈尔医学院 | A kind of integrated sterilizing storage cabinet |
| CN204602232U (en) * | 2015-03-18 | 2015-09-02 | 青岛银泰洁净设备有限公司 | A kind of ultra-clean workbench |
| CN204634461U (en) * | 2015-04-17 | 2015-09-16 | 彭阳县福泰菌业有限责任公司 | Edible mushroom aseptic inoculation and preculture air cleaning system |
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| CN105062884A (en) * | 2015-08-31 | 2015-11-18 | 东北大学 | Enrichment and cultivation device for hydrogen-producing bacteria through photo-fermentation and using method thereof |
| CN205232874U (en) * | 2015-10-30 | 2016-05-18 | 重庆市洪欣食用菌有限公司 | Domestic fungus disinfects and filters culture tank |
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| CN107182550A (en) * | 2017-06-25 | 2017-09-22 | 江苏福利达农业科技有限公司 | A kind of gas circulating device for cultivating edible mushrooms |
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|---|---|
| CN106244448B (en) | 2019-04-09 |
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