CN106236816A - A kind of extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid - Google Patents
A kind of extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid Download PDFInfo
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- CN106236816A CN106236816A CN201610677875.4A CN201610677875A CN106236816A CN 106236816 A CN106236816 A CN 106236816A CN 201610677875 A CN201610677875 A CN 201610677875A CN 106236816 A CN106236816 A CN 106236816A
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- miq
- alkaloid
- herba hylotelephii
- hylotelephii erythrosticti
- extracting method
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/41—Crassulaceae (Stonecrop family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The present invention relates to the extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid, comprise the steps: to extract: after being pulverized by Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti, soaked in solvent, by filtering after soak supersound process, take filter liquor；Concentrate: by filter liquor concentrating under reduced pressure, take concentrated solution；Dry: concentrated solution is dried；Wherein, solvent is water or ethanol solution that mass concentration is 55%～65%.The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid according to the present invention, process is simple, extracts content high, effectively raises the extraction efficiency of alkaloid, have good using value.
Description
Technical field
The present invention relates to medicinal chemistry arts, in particular it relates to the extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid.
Background technology
Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti, has another name called Sedum spectabile Boreau, magnificent Herba hylotelephii erythrosticti, Sedum spectabile etc., is commonly called as Hylotelephium erythrostictum (Miq.) H.Ohba, HUDOU seven, and Radix Notoginseng of invigorating blood circulation, is scape
It section sengreen.With all herbal medicine, can adopt the whole year, many using fresh herbs.Its property is flat, bitter in the mouth, micro-acid, there is expelling wind and removing dampness, invigorate blood circulation
Effect of blood stasis dispelling, hemostasis and pain-relieving and heat-clearing and toxic substances removing.Research shows containing abundant nutrient substance in sengreen, such as biology
Alkali, steroidal, tannin, flavone, condensed tannin and raw cyanogen compound etc..Wherein alkaloid compound has antiinflammatory, antibacterial, disease-resistant
Poison, protect the liver, many pharmacologically actives such as anticancer.
The extracting method of existing Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is more single, and the content of the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid extracted also compares
Low.It would therefore be highly desirable to a kind of more economically, effective and easy method extract the alkaloid in Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti.
Summary of the invention
It is an object of the present invention to propose a kind of economy, the extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid effective, simple.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid according to embodiments of the present invention, comprises the steps: to extract: by eight
After precious Herba hylotelephii erythrosticti is pulverized, soaked in solvent, by filtering after soak supersound process, take filter liquor；Concentrate: filter liquor is reduced pressure dense
Contracting, takes concentrated solution；Dry: concentrated solution is dried；Wherein, solvent is water or ethanol solution that mass concentration is 55%～65%.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid according to embodiments of the present invention, process is simple, extracts content high, has
The extraction efficiency that improve alkaloid of effect, has good using value.
It addition, the extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid according to the above embodiment of the present invention, it is also possible to have as
Lower additional technical characteristic:
Further, in extraction step, after Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is pulverized, soaked in solvent, obtain the first soak, by first
Filter after soak supersound process, obtain the first filter liquor and the first filter cake；Soaked in solvent the first filter cake, obtains the second immersion
Liquid, by filtering after the second soak supersound process, obtains the second filter liquor and the second filter cake；Soaked in solvent the second filter cake,
To the 3rd soak, by filtering after the 3rd soak supersound process, obtain the 3rd filter liquor and the 3rd filter cake；Leach first
Liquid, the second filter liquor and the 3rd filter liquor merge, and obtain filter liquor；Wherein, solvent is water or mass concentration is 55%～65%
Ethanol solution.
Advantageously, water is distilled water.
Advantageously, the mass concentration of ethanol solution is 60%.
Advantageously, when solvent is water, in extraction step, the temperature of supersound process is 75 DEG C～80 DEG C；Solvent is ethanol
Time, in extraction step, the temperature of supersound process is 65 DEG C～75 DEG C.
Advantageously, in extraction step, the parts by weight of solvent are 20 parts～40 parts.
Advantageously, in extraction step, the time of supersound process is 20min～40min.
Advantageously, in baking step, drying temperature is 65 DEG C～85 DEG C.
Advantageously, in extraction step, the time of solvent soaking is 1h～3h.
Advantageously, in concentrating under reduced pressure step, the temperature of concentrating under reduced pressure is 50 DEG C～65 DEG C.
The additional aspect of the present invention and advantage will part be given in the following description, and part will become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Detailed description of the invention
Embodiments of the invention are described below in detail.The embodiments described below is exemplary, it is intended to be used for explaining this
Invention, and be not considered as limiting the invention.
Embodiment 1
Weigh the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti that 1kg is dried, be ground into coarse powder, add 30kg 60% ethanol solution, soak after 2h
Supersound process 30min at a temperature of 65 DEG C, by filtered through gauze, obtains the first filtrate and the first filter cake；Add in the first filter cake again
The ethanol solution of the 60% of 30kg, supersound process 30min at a temperature of 65 DEG C, by filtered through gauze, obtain the second filtrate and second
Filter cake；Again toward the ethanol solution of add 30kg in the second filter cake 60%, supersound process 30min at a temperature of 65 DEG C, use gauze
Filter to obtain the 3rd filtrate and the 3rd filter cake.Merge the first filtrate, the second filtrate and the 3rd filtrate, and move in Rotary Evaporators
Concentrating at a temperature of 50 DEG C, concentrated solution moves in the culture dish weighed, and then dries at a temperature of 65 DEG C in thermostatic drying chamber and claims
Weight.
The content of the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid of the different cultivars that table 1 embodiment 1 is extracted
| Sequence number | Place | Color | Alkaloid |
| 1 | Hai Hu new district Wen Huilu | Bright red | 0.121% |
| 2 | Nursery, the south of a city | Hemerocallis citrina Baroni | 0.077% |
| 3 | Nursery, the south of a city | Bright red | 0.078% |
| 4 | Nursery, the south of a city | Plum | 0.260% |
| 5 | Nursery, the south of a city | White | 0.312% |
Embodiment 2
Weighing the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti that 1kg is dried, be ground into coarse powder, the ethanol solution of the 65% of addition 30kg, after soaking 1.5h
Supersound process 30min at a temperature of 75 DEG C, obtains the first filtrate and the first filter cake by filtered through gauze；Add in the first filter cake again
60% ethanol solution of 30kg, supersound process 30min at a temperature of 75 DEG C, obtain the second filtrate and the second filter cake by filtered through gauze；
Again toward the second filter cake adds 60% ethanol solution of 30kg, supersound process 30min at a temperature of 75 DEG C, obtain by filtered through gauze
Three filtrates and the 3rd filter cake.Merge the first filtrate, the second filtrate and the 3rd filtrate and move in Rotary Evaporators 65 DEG C of temperature
Lower concentration, concentrated solution moves in the culture dish weighed, and then dries at a temperature of 85 DEG C in thermostatic drying chamber and weighs.
The content of the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid of the different cultivars that table 2 embodiment 2 is extracted
| Sequence number | Place | Color | Content |
| 1 | Hai Hu new district Wen Huilu | Bright red | 0.102% |
| 2 | Nursery, the south of a city | Hemerocallis citrina Baroni | 0.067% |
| 3 | Nursery, the south of a city | Bright red | 0.058% |
| 4 | Nursery, the south of a city | Plum | 0.240% |
| 5 | Nursery, the south of a city | White | 0.307% |
Embodiment 3
The dry Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti weighing 1kg is ground into coarse powder, adds distilled water supersound extraction at 80 DEG C of 30kg
30min.It is repeated 2 times the process of supersound extraction, and filters after extracting solution is merged, move in Rotary Evaporators at a temperature of 60 DEG C
Concentrating, concentrated solution moves in the culture dish weighed, and then dries at a temperature of 70 DEG C in thermostatic drying chamber and weighs.
The weight of the Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid of the different cultivars that table 3 embodiment 3 is extracted
| Sequence number | Place | Color | Content |
| 1 | Hai Hu new district Wen Huilu | Bright red | 0.047% |
| 2 | Nursery, the south of a city | Hemerocallis citrina Baroni | 0.057% |
| 3 | Nursery, the south of a city | Bright red | 0.028% |
| 4 | Nursery, the south of a city | Plum | 0.117% |
| 5 | Nursery, the south of a city | White | 0.288% |
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid according to embodiments of the present invention, process is simple, extracts content high, has
The extraction efficiency that improve alkaloid of effect, has good using value.
In the description of this specification, reference term " embodiment ", " some embodiments ", " example ", " specifically show
Example " or the description of " some examples " etc. means to combine this embodiment or example describes specific features, structure, material or spy
Point is contained at least one embodiment or the example of the present invention.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.And, the specific features of description, structure, material or feature can be in office
One or more embodiments or example combine in an appropriate manner.Additionally, in the case of the most conflicting, the skill of this area
The feature of the different embodiments described in this specification or example and different embodiment or example can be tied by art personnel
Close and combination.
Although above it has been shown and described that embodiments of the invention, it is to be understood that above-described embodiment is example
Property, it is impossible to being interpreted as limitation of the present invention, those of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, revises, replaces and modification.
Claims (10)
1. the extracting method of a Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid, it is characterised in that comprise the steps:
Extract: after Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is pulverized, soaked in solvent, by filtering after described soak supersound process, take filter liquor；
Concentrate: by described filter liquor concentrating under reduced pressure, take concentrated solution；
Dry: described concentrated solution is dried；
Wherein, described solvent is water or ethanol solution that mass concentration is 55%～65%.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid the most according to claim 1, it is characterised in that
In described extraction step, after Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti is pulverized, soaked in solvent, obtain the first soak, soak described first
Filter after liquid supersound process, obtain the first filter liquor and the first filter cake；
Soaked in solvent the first filter cake, obtains the second soak, by filtering after described second soak supersound process, obtains second
Filter liquor and the second filter cake；
Soaked in solvent the second filter cake, obtains the 3rd soak, by filtering after described 3rd soak supersound process, obtains the 3rd
Filter liquor and the 3rd filter cake；
Described first filter liquor, described second filter liquor and described 3rd filter liquor are merged, obtains filter liquor；Wherein, described
Solvent is water or ethanol solution that mass concentration is 55%～65%.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid the most according to claim 1 and 2, it is characterised in that described water is
Distilled water.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid the most according to claim 1 and 2, it is characterised in that described ethanol
The mass concentration of solution is 60%.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid the most according to claim 1 and 2, it is characterised in that described solvent
During for water, in described extraction step, the temperature of described supersound process is 75 DEG C～80 DEG C；When described solvent is ethanol, in institute
Stating in extraction step, the temperature of described supersound process is 65 DEG C～75 DEG C.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid the most according to claim 1 and 2, it is characterised in that carry described
Taking in step, the parts by weight of described solvent are 20 parts～40 parts.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid the most according to claim 1 and 2, it is characterised in that carry described
Taking in step, the time of described supersound process is 20min～40min.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid the most according to claim 1 and 2, it is characterised in that in described baking
In dry step, described drying temperature is 65 DEG C～85 DEG C.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid the most according to claim 1 and 2, it is characterised in that carry described
Taking in step, the time of described solvent soaking is 1h～3h.
The extracting method of a kind of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid the most according to claim 1 and 2, it is characterised in that subtract described
In pressure concentration step, the temperature of concentrating under reduced pressure is 50 DEG C～65 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610677875.4A CN106236816A (en) | 2016-08-16 | 2016-08-16 | A kind of extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid |
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| CN201610677875.4A CN106236816A (en) | 2016-08-16 | 2016-08-16 | A kind of extracting method of Hylotelephium erythrostictum (Miq.) H.Ohba Herba hylotelephii erythrosticti alkaloid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3099992A1 (en) * | 2019-08-21 | 2021-02-26 | Laboratoires De Biologie Vegetale Yves Rocher | COSMETIC USE OF A SPECTABILE SEDUM EXTRACT AS A SLIMMING AGENT, AND COMPOSITIONS CONTAINING SUCH EXTRACT |
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| KR200451591Y1 (en) * | 2008-06-24 | 2010-12-27 | 주식회사 바낙스 | Brake apparatus of bait cast reel |
| CN103141263A (en) * | 2013-03-20 | 2013-06-12 | 山西省农业科学院园艺研究所 | Breeding method for Sedum Spectabile Boreau |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3099992A1 (en) * | 2019-08-21 | 2021-02-26 | Laboratoires De Biologie Vegetale Yves Rocher | COSMETIC USE OF A SPECTABILE SEDUM EXTRACT AS A SLIMMING AGENT, AND COMPOSITIONS CONTAINING SUCH EXTRACT |
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