CN106226302A - NBAP detection card - Google Patents
NBAP detection card Download PDFInfo
- Publication number
- CN106226302A CN106226302A CN201610783727.0A CN201610783727A CN106226302A CN 106226302 A CN106226302 A CN 106226302A CN 201610783727 A CN201610783727 A CN 201610783727A CN 106226302 A CN106226302 A CN 106226302A
- Authority
- CN
- China
- Prior art keywords
- window
- nbap
- detection card
- groove
- slide plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
Abstract
The present invention provides a kind of NBAP detection card.Described NBAP detection card includes substrate, and its surface is provided with multiple groove；Cover plate, is covered on described substrate, the lower surface corresponding with described upper surface including upper surface and run through described upper surface and described lower surface and spaced filter window and Process window, groove described in the one_to_one corresponding respectively of its position；Sample filter, is located at the upper surface of described cover plate, and including running through the sample-adding window of its surface configuration, its position is just to described filter window；And reaction film slide plate, it is located at the lower surface of described cover plate, including running through the slide plate window of its surface configuration and covering in the reaction film of described slide plate window, the position of described slide plate window is just to described filter window.It is short that the described NBAP detection card that the present invention provides has the detection used time, and blood sampling volume is little, simple operation and other advantages.
Description
Technical field
The present invention relates to in-vitro diagnosis detection technique field, be specifically related to a kind of NBAP detection card.
Background technology
Bone alkaline phosphatase, i.e. NBAP (NBAP), is secreted by osteoblast, is osteoblastic
One of phenotypic marker, it can directly reflect osteoblastic activity or function status.During rickets osteoblast proliferation active,
Piling up, its secreted bone alkaline phosphatase increases, and is mainly used in the special of rickets of child early diagnosis and subclinical discriminating
Property reference index, be also be currently used for evaluate human bone mineralising obstacle optimal parameter.NBAP is by sclerotin
In be secreted, when calcium precipitation deficiency in bone, this enzyme secretion increases, and just secretes minimizing in bone during calcium salt abundance, so
It is used for helping to check for calcium absorption not enough.NBAP catalysis activity in detection children's's blood, nationality is with examination or auxiliary
Help the metabolic osteopathy that the bone calcification obstacle that causes because of calcium malnutrition of diagnosis or other reasons cause.Bone alkaline phosphatase is normal
Reference value is as follows: normal level≤200u/L；Prevent horizontal 250u/L；Medical level 300u/L.
At present, in serum, the method that predominantly detects of bone alkaline phosphatase content includes that electrophoresis method, agglutinin agglutination, enzyme join
Immunization etc..But, these detection methods above-mentioned or needs rely on corresponding complicated instrument and equipment, or required sample is
The defects such as serum, accordingly, there exist blood using amount big, and detecting step is complicated, detection speed is slow, are not suitable for this bone alkalescence of infant
The abnormal colony occurred frequently of phosphatase.
Summary of the invention
For solving the defect that above-mentioned prior art exists, the present invention provides a kind of by gathering children's's finger tip blood, can be fast
Speed detects the NBAP detection card of enzymatic activity height.
The present invention provides a kind of NBAP detection card, and including substrate, its surface is provided with multiple groove；Lid
Plate, is covered on described substrate, and described cover plate includes the upper surface lower surface corresponding with described upper surface and runs through on described
Surface and described lower surface and spaced filter window and Process window, described filter window and the position of described Process window
Put groove described in difference one_to_one corresponding；Sample filter, is located at the upper surface of described cover plate, and described sample filter includes running through
The sample-adding window of its surface configuration, the position of described sample-adding window is just to described filter window；And reaction film slide plate, it is located at described
The lower surface of cover plate, described reaction film slide plate includes the slide plate window running through its surface configuration and covers in described slide plate window
Reaction film, the position of described slide plate window is just to described filter window, and described reaction film is contained within adsorbing bone source property alkaline phosphatase
The agglutinin of enzyme.
The described NBAP provided in the present invention detects in a kind of preferred embodiment blocked, and described groove is
Recessed groove, it is internally caved in by described substrate one side surface and forms.
The described NBAP provided in the present invention detects in a kind of preferred embodiment blocked, and described groove is
Projecting slot, it is formed by described substrate one side surface outside portion projection.
The described NBAP provided in the present invention detects in a kind of preferred embodiment blocked, described projecting slot
For cup-like structure.
The described NBAP provided in the present invention detects in a kind of preferred embodiment blocked, wherein described in one
Adsorptive pads it is provided with in groove.
The described NBAP provided in the present invention detects in a kind of preferred embodiment blocked, described substrate bag
Including the buckle being located at its edge, described cover plate includes the draw-in groove being located at its edge, and described cover plate and described substrate are by described card
Detain and realize covering being connected with described draw-in groove.
The described NBAP provided in the present invention detects in a kind of preferred embodiment blocked, described sample mistake
The transverse cross-sectional shape of filter is circular, square and any one in triangle.
The described NBAP provided in the present invention detects in a kind of preferred embodiment blocked, described filtering window
The shape of mouth, Process window, sample-adding window and slide plate window be circle, square, octahedral shape and trapezoidal in any one.
Compared to prior art, what the described NBAP detection that the present invention provides blocked has the beneficial effects that:
Efficiently solving the drawback that current test kit generally exists, it is short that it has the detection used time, and blood sampling volume is little, it is only necessary to finger tip blood, no
Need to rely on complicated instrument and equipment, simple operation and other advantages, be especially suitable for this bone alkaline phosphatase of infant abnormal occurred frequently
Colony, is suitable for large-scale promotion application.
Accompanying drawing explanation
For the technical scheme being illustrated more clearly that in the embodiment of the present invention, embodiment will be described below used in
Accompanying drawing is briefly described, it should be apparent that, the accompanying drawing in describing below is only some embodiments of the present invention, for ability
From the point of view of the those of ordinary skill of territory, on the premise of not paying creative work, it is also possible to obtain the attached of other according to these accompanying drawings
Figure, wherein:
Fig. 1 is the structural representation of NBAP detection card one preferred embodiment that the present invention provides；
Fig. 2 is the sectional view of the detection card of NBAP shown in Fig. 1.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Describe, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments wholely.
Please refer to Fig. 1 and Fig. 2, wherein Fig. 1 is that the NBAP detection card one that the present invention provides is preferable
The structural representation of embodiment, Fig. 2 is the sectional view of the detection card of NBAP shown in Fig. 1.
Described NBAP detection card 1 includes substrate 11, cover plate 13, sample filter 15 and reaction film slide plate
17。
The surface of described substrate 11 is provided with two grooves 111, is wherein provided with adsorptive pads in groove 111 described in.This enforcement
In example, described groove 111 is recessed groove, and it is internally caved in by described substrate 11 1 side surface and forms；In other cases,
Described groove 111 can be also projecting slot and structure is cup-like structure, its by the outside portion of described substrate 11 1 side surface protruding and
Become.
Described cover plate 13 is covered on described substrate 11.Described cover plate 13 includes that upper surface 131 is corresponding with described upper surface
Lower surface 133 and run through described upper surface 131 and described lower surface 133 and spaced filter window 135 and reaction window
Mouth 137, groove 111 described in one_to_one corresponding is distinguished in the position of described filter window 135 and described Process window 137.Described substrate
11 include the buckle being located at its edge, and described cover plate 13 includes the draw-in groove being located at its edge, described cover plate 13 and described substrate 11
Realize covering being connected with described draw-in groove by described buckle.
Described sample filter 15 is located at the upper surface 131 of described cover plate 13.Described sample filter 15 includes running through it
The sample-adding window 151 of surface configuration, the position of described sample-adding window 151 is just to described filter window 135.Described sample filter
The transverse cross-sectional shape of 15 is circular, square and any one in triangle.
Described reaction film slide plate 17 is located at the lower surface 133 of described cover plate 13.Described reaction film slide plate 17 includes running through it
The slide plate window 171 of surface configuration and covering in the reaction film 173 of described slide plate window 171, the position of described slide plate window 171
Just to described filter window 135, described reaction film 173 is contained within adsorbing the agglutinin of NBAP.Described filtration
Window 135, Process window 137, sample-adding window 151 and the shape of slide plate window 171 are circle, square, octahedral shape and trapezoidal
In any one.
During described NBAP detection card 1 use that the present invention provides, gather blood according to common detection methods
Liquid sample, then instills the sample handled well in detection card sample-adding window, is adding corresponding nitrite ion and stop buffer, after reaction
Showing color on reaction film, colored intensity is relevant to the bone alkaline phosphatase content in tested blood sample.
Having the beneficial effects that of the described NBAP detection card 1 that the present invention provides: efficiently solve at present
The drawback that test kit generally exists, it is short that it has the detection used time, and blood sampling volume is little, it is only necessary to finger tip blood, it is not necessary to relies on complexity
Instrument and equipment, simple operation and other advantages, it is especially suitable for the abnormal colony occurred frequently of this bone alkaline phosphatase of infant, is suitable for extensive
Popularization and application.
The foregoing is only embodiments of the invention, not thereby limit the scope of the claims of the present invention, every utilize this
Equivalent structure or equivalence flow process that bright description is made convert, or are directly or indirectly used in other relevant technology neck
Territory, within being the most in like manner included in the scope of patent protection of the present invention.
Claims (8)
1. a NBAP detection card, it is characterised in that: include
Substrate, its surface is provided with multiple groove；
Cover plate, is covered on described substrate, and described cover plate includes the upper surface lower surface corresponding with described upper surface and runs through
Described upper surface and described lower surface and spaced filter window and Process window, described filter window and described reaction window
Groove described in the one_to_one corresponding respectively of the position of mouth；
Sample filter, is located at the upper surface of described cover plate, and described sample filter includes the sample-adding window running through its surface configuration
Mouthful, described sample-adding the window's position is just to described filter window；And reaction film slide plate, be located at the lower surface of described cover plate, described instead
Film slide plate is answered to include running through the slide plate window of its surface configuration and cover in the reaction film of described slide plate window, described slide plate window
Position just to described filter window, described reaction film be contained within adsorb NBAP agglutinin.
NBAP the most according to claim 1 detection card, it is characterised in that: described groove is recessed groove,
It is internally caved in by described substrate one side surface and forms.
NBAP the most according to claim 1 detection card, it is characterised in that: described groove is projecting slot,
It is formed by described substrate one side surface outside portion projection.
NBAP the most according to claim 3 detection card, it is characterised in that: described projecting slot is cup-shaped knot
Structure.
NBAP the most according to claim 1 detection card, it is characterised in that: wherein set in groove described in
There is adsorptive pads.
NBAP the most according to claim 1 detection card, it is characterised in that: described substrate includes being located at it
The buckle at edge, described cover plate includes that the draw-in groove being located at its edge, described cover plate and described substrate pass through described buckle with described
Draw-in groove realizes covering connection.
NBAP the most according to claim 1 detection card, it is characterised in that: the horizontal stroke of described sample filter
Cross-sectional shapes is circular, square and any one in triangle.
NBAP the most according to claim 1 detection card, it is characterised in that: described filter window, reaction
Window, sample-adding window and the shape of slide plate window be circle, square, octahedral shape and trapezoidal in any one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610783727.0A CN106226302A (en) | 2016-08-31 | 2016-08-31 | NBAP detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610783727.0A CN106226302A (en) | 2016-08-31 | 2016-08-31 | NBAP detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106226302A true CN106226302A (en) | 2016-12-14 |
Family
ID=58072436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610783727.0A Pending CN106226302A (en) | 2016-08-31 | 2016-08-31 | NBAP detection card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106226302A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06273419A (en) * | 1993-03-18 | 1994-09-30 | Mochida Pharmaceut Co Ltd | Simple measuring device and method |
| CN2316658Y (en) * | 1997-11-21 | 1999-04-28 | 北京远东协和生物技术有限公司 | Diagnostic testing strip for alkali phosphoase |
| US20060062688A1 (en) * | 2004-02-03 | 2006-03-23 | Polymer Technology Systems, Inc. | Bodily fluid analysis system |
| CN103630686A (en) * | 2013-11-29 | 2014-03-12 | 电子科技大学 | Test strip card capable of automatically filtering sample |
| CN105445454A (en) * | 2015-11-20 | 2016-03-30 | 广州瑞博奥生物科技有限公司 | Quantifiable immunochromatography device |
| CN206057189U (en) * | 2016-08-31 | 2017-03-29 | 长沙励思生物技术有限公司 | NBAP detection card |
-
2016
- 2016-08-31 CN CN201610783727.0A patent/CN106226302A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06273419A (en) * | 1993-03-18 | 1994-09-30 | Mochida Pharmaceut Co Ltd | Simple measuring device and method |
| CN2316658Y (en) * | 1997-11-21 | 1999-04-28 | 北京远东协和生物技术有限公司 | Diagnostic testing strip for alkali phosphoase |
| US20060062688A1 (en) * | 2004-02-03 | 2006-03-23 | Polymer Technology Systems, Inc. | Bodily fluid analysis system |
| CN103630686A (en) * | 2013-11-29 | 2014-03-12 | 电子科技大学 | Test strip card capable of automatically filtering sample |
| CN105445454A (en) * | 2015-11-20 | 2016-03-30 | 广州瑞博奥生物科技有限公司 | Quantifiable immunochromatography device |
| CN206057189U (en) * | 2016-08-31 | 2017-03-29 | 长沙励思生物技术有限公司 | NBAP detection card |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161214 |
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| RJ01 | Rejection of invention patent application after publication |