CN106192459A - A kind of utilize the prodigiosin colouring method to silk fabric - Google Patents
A kind of utilize the prodigiosin colouring method to silk fabric Download PDFInfo
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- CN106192459A CN106192459A CN201610534232.4A CN201610534232A CN106192459A CN 106192459 A CN106192459 A CN 106192459A CN 201610534232 A CN201610534232 A CN 201610534232A CN 106192459 A CN106192459 A CN 106192459A
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- prodigiosin
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
- D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
- D06P1/34—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using natural dyestuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
- D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
- D06P1/44—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using insoluble pigments or auxiliary substances, e.g. binders
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
- D06P3/00—Special processes of dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form, classified according to the material treated
- D06P3/02—Material containing basic nitrogen
- D06P3/04—Material containing basic nitrogen containing amide groups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Textile Engineering (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
A kind of utilize the prodigiosin colouring method to silk fabric, have adjusted the culture process of original prodigiosin, it is better that the prodigiosin making cultivation is applied to silk fabric, particularly dye heavier in the case of, time needed for the consumption of the prodigiosin added and dyeing all has greatly improved, without adding the chemical reagent such as mordant in dyeing course, low cost, health environment-friendly.
Description
Technical field
The present invention relates to technical field of textile printing, particularly relate to a kind of utilize the prodigiosin dyeing side to silk fabric
Method.
Background technology
In recent years, due to environmental problem and the impact of energy problem, people tend to pursuing continuable, eco-friendly and
Human health advantageous approach is produced dyestuff.Exploitation natural dye is the general trend of world's application dyestuff development, natural dye
Material refers to obtain from plant, animal or mineral resources, the little or no dyestuff through chemical process, a lot of natural dyes
Having safe and reliable, tone is natural, close to advantages such as natural materials, and the trend being dyestuff and developing natural, multi-functional.Therefore from certainly
So resource filters out the microorganism that can produce natural dye, and then the natural dye of developing new product variety, especially there is merit
The natural dye of energy property, has broad prospects.Along with the development of biotechnology, utilize biotechnology to produce natural dye and behave
Open wide field.Biofermentation product stability is high, and specificity is strong, pollution-free, production process to people's fanout free region, because of
This, biological fermentation process is a kind of method of good synthetic dyestuffs.
The natural pigment of the methoxypyrrole framing structure that bacterial dye prodigiosin is made up of three pyrrole rings, is one
A little actinomycetes, Serratieae and the secondary metabolite of other antibacterials, have immunosuppressant, antibacterium, antifungal and malaria
Etc. multiple biological activity.Therefore, bacterial dye prodigiosin just has antibacterial function as its dyed fabric after dyeing,
And there is anticancer effect.
Donghua University discloses prodigiosin to Pilus Caprae seu Ovis, the dyeing side of acrylon in four patents of 2010 and 2011 applications
Method, but the colouring method of silk fiber is not studied.
Summary of the invention
In order to solve the problem in background technology, the present invention provides a kind of and utilizes the prodigiosin dyeing side to silk fabric
Method.
A kind of utilize the prodigiosin colouring method to silk fabric, comprise the following steps:
A, dissolve the prodigiosin by by ethyl acetate, and add Elymus nutans extract, be then added in water, make
Staining solution；
B, using above-mentioned staining solution to dye silk fabric, the consumption of prodigiosin is silk fabric mass fraction
0.1-0.5%, dye bath ratio is 1: 10-20, regulation dye liquor pH value be 4.0-6.0, dyeing temperature is 60-70 DEG C, insulation
Time is 15-25min.
After C, dyeing terminate, by fabric washing, 120 DEG C of drying 3-5min after insulation is terminated,.
Preferably, the preparation method of described prodigiosin, comprise the following steps:
Step one: the strain (Serratia marcesens, purchased from Chinese medicine antibacterial preservation administrative center) bought is carried out dilute
Release, after will dilution after antibacterial coat put on plating medium in constant incubator cultivate；
Step 2: picking red pigment and the deeper bacterial strain of color are coated on plating medium, and by this bacterial strain at 4 DEG C
Under the conditions of preserve.
Step 3: the bacterial strain screened is cultivated in seed culture medium 6-8h, the rear fermentation medium that accesses is cultivated
15h；
Step 4: respectively supernatant and thalline are separated；Supernatant directly by ethyl acetate by prodigiosin from water
Extracting in solution, flocculate extracts after concentrating by rotary evaporator after being first centrifuged in acidic methanol.
Plating medium in described step one, including following main component: yeast powder 10g/L, peptone 20g/L,
Glucose 2g/L, agar powder 20g/L, constant volume to 1L, surplus is water；
The described seed culture medium in step 3, including following main component: sucrose 15g/L, yeast extract 0.5g/L,
Sodium chloride 0.2g/L, magnesium sulfate 1g/L, constant volume to 1L, surplus is water.
Preferably, described silk fabric needs before use through sterilization processing.
Preferably, described bactericidal treatments is: 150 DEG C of high temperature sterilize 10min.
Elymus nutans is Perennual herbaceous plant, high 50-70 centimetre, cultigen 80-120 centimetre.Rhizome is dredged
Plexi, well developed root system, in palpus shape.Stalk is upright, and 3-4 saves, and it is bent that base portion saves slightly knee joint.Blade is flat, long 6-8 centimetre, wide 3-5 millimeter,
The micro-preesed grass in both sides or bottom smooth, and dredge raw pubescence above, and sheath is in addition to base portion person, and remaining is all shorter than internode.The tip of a leaf is extremely short, is about
0.5 millimeter.Spike arrangement is relatively tight, the side of the many relatively cobs of small ear, and generally curved, tip is sagging, long 5-12 centimetre,
Cob often saves typically two pieces of small ears；Respectively save close to top end only tool one piece of small ear, base portion have sterile small ear, small ear stalk short or
Nothing；Small ear is green, and maturation zone purple, long 12-15 millimeter, every small ear little Hua Han 3-4, the most only 2-3 flower can be educated.Grain husk is in oval
Shape, has 3-4 arteries and veins, long 4-5 millimeter, the short awns that tool 1-4 millimeter is long；The long lanceolar of lemma, has 5 arteries and veins；Awn length 12-20 millimeter, coarse,
Outwards contrary flexure or slightly launch, glumelle and lemma have 5 arteries and veins, isometric, tip blunt circle or truncated.Turn black after flower pesticide maturation.Caryopsis, plants
Sub-lanceolar, puce, mass of 1000 kernel 2.85-3.2 gram.
Elymus nutans is internal containing flavone compound after deliberation, adds Elymus nutans in the present invention, on the one hand can
On the other hand to improve Color, the anti-microbial property of silk fabric after dyeing can be promoted.
Of the present invention utilize the prodigiosin colouring method to silk fabric, have adjusted the training of original prodigiosin
Support technique, make the prodigiosin of cultivation be applied to silk fabric better, particularly dye heavier in the case of, the spirit of addition
Time needed for the consumption of bacterioruberin and dyeing all has greatly improved, without adding the chemistry examinations such as mordant in dyeing course
Agent, low cost, health environment-friendly.
Detailed description of the invention
Embodiment 1
A kind of utilize the prodigiosin colouring method to silk fabric, comprise the following steps:
A, dissolve the prodigiosin by by ethyl acetate, and add Elymus nutans extract, be then added in water, make
Staining solution；
B, using above-mentioned staining solution to dye silk fabric, the consumption of prodigiosin is silk fabric mass fraction
0.4%, dye bath ratio is 1: 15, regulation dye liquor pH value be 4.0-6.0, dyeing temperature is 65 DEG C, and temperature retention time is
20min。
After C, dyeing terminate, by fabric washing, 120 DEG C of drying 4min after insulation is terminated,.
The preparation method of described prodigiosin, comprises the following steps:
Step one: the strain (Serratia marcesens, purchased from Chinese medicine antibacterial preservation administrative center) bought is carried out dilute
Release, after will dilution after antibacterial coat plating medium (yeast powder 10g/L, peptone 20g/L, glucose 2g/L, agar powder
Put on 20g/L) in constant incubator and cultivate；
Step 2: picking red pigment and the deeper bacterial strain of color are coated on plating medium, and by this bacterial strain at 4 DEG C
Under the conditions of preserve.
Step 3: by the bacterial strain that screens at seed culture medium (sucrose 15g/L, yeast extract 0.5g/L, sodium chloride
0.2g/L, magnesium sulfate 1g/L, adjust pH value 7.0-7.5) the middle 6h that cultivates, the rear fermentation medium that accesses cultivates 15h；
Step 4: respectively supernatant and thalline are separated；Supernatant directly by ethyl acetate by prodigiosin from water
Extracting in solution, flocculate extracts after concentrating by rotary evaporator after being first centrifuged in acidic methanol.
Described silk fabric needs through sterilization processing before use, and described bactericidal treatments is: 150 DEG C of high temperature
Sterilizing 10min.
Embodiment 2
A kind of utilize the prodigiosin colouring method to silk fabric, comprise the following steps:
A, dissolve the prodigiosin by by ethyl acetate, and add Elymus nutans extract, be then added in water, make
Staining solution；
B, using above-mentioned staining solution to dye silk fabric, the consumption of prodigiosin is silk fabric mass fraction
0.5%, dye bath ratio is 1: 20, regulation dye liquor pH value be 4.0-5.0, dyeing temperature is 65 DEG C, and temperature retention time is
25min。
After C, dyeing terminate, by fabric washing, 120 DEG C of drying 3min after insulation is terminated,.
The preparation method of described prodigiosin, comprises the following steps:
Step one: the strain (Serratia marcesens, purchased from Chinese medicine antibacterial preservation administrative center) bought is carried out dilute
Release, after will dilution after antibacterial coat plating medium (yeast powder 10g/L, peptone 20g/L, glucose 2g/L, agar powder
Put on 20g/L) in constant incubator and cultivate；
Step 2: picking red pigment and the deeper bacterial strain of color are coated on plating medium, and by this bacterial strain at 4 DEG C
Under the conditions of preserve.
Step 3: by the bacterial strain that screens at seed culture medium (sucrose 15g/L, yeast extract 0.5g/L, sodium chloride
0.2g/L, magnesium sulfate 1g/L, adjust pH value 7.0-7.5) the middle 7h that cultivates, the rear fermentation medium that accesses cultivates 15h；
Step 4: respectively supernatant and thalline are separated；Supernatant directly by ethyl acetate by prodigiosin from water
Extracting in solution, flocculate extracts after concentrating by rotary evaporator after being first centrifuged in acidic methanol.
Described silk fabric needs through sterilization processing before use, and described bactericidal treatments is: 150 DEG C of high temperature
Sterilizing 10min.
Embodiment 3
A kind of utilize the prodigiosin colouring method to silk fabric, comprise the following steps:
A, dissolve the prodigiosin by by ethyl acetate, and add Elymus nutans extract, be then added in water, make
Staining solution；
B, using above-mentioned staining solution to dye silk fabric, the consumption of prodigiosin is silk fabric mass fraction
0.1%, dye bath ratio is 1: 10, regulation dye liquor pH value be 4.0-5.5, dyeing temperature is 60 DEG C, and temperature retention time is
15min。
After C, dyeing terminate, by fabric washing, 120 DEG C of drying 5min after insulation is terminated,.
The preparation method of described prodigiosin, comprises the following steps:
Step one: the strain (Serratia marcesens, purchased from Chinese medicine antibacterial preservation administrative center) bought is carried out dilute
Release, after will dilution after antibacterial coat plating medium (yeast powder 10g/L, peptone 20g/L, glucose 2g/L, agar powder
Put on 20g/L) in constant incubator and cultivate；
Step 2: picking red pigment and the deeper bacterial strain of color are coated on plating medium, and by this bacterial strain at 4 DEG C
Under the conditions of preserve.
Step 3: by the bacterial strain that screens at seed culture medium (sucrose 15g/L, yeast extract 0.5g/L, sodium chloride
0.2g/L, magnesium sulfate 1g/L, adjust pH value 7.0-7.5) the middle 8h that cultivates, the rear fermentation medium that accesses cultivates 15h；
Step 4: respectively supernatant and thalline are separated；Supernatant directly by ethyl acetate by prodigiosin from water
Extracting in solution, flocculate extracts after concentrating by rotary evaporator after being first centrifuged in acidic methanol.
Described silk fabric needs through sterilization processing before use, and described bactericidal treatments is: 150 DEG C of high temperature
Sterilizing 10min.
The silk fabric using embodiment of the present invention 1-3 method is detected, and by the parameter in dyeing and east Hua Da
Learn disclosed CN102493228A prodigiosin the colouring method of Pilus Caprae seu Ovis is contrasted, obtain following data:
Comparative example 1
Being removed by Elymus nutans extract in embodiment 1, other conditions are constant, test its Color and antibiotic property
Can, obtain following data:
| Embodiment 1 | Comparative example 1 | |
| WASHING COLOR FASTNESS | 4 grades | 4 grades |
| Dry fastness | 4 grades | 3 grades |
| Fastness to wet rubbing | 4 grades | 3 grades |
| Biocidal property (Candida albicans) | 98 | 85 |
| Biocidal property (escherichia coli) | 95 | 80 |
| Biocidal property (staphylococcus aureus) | 97 | 90 |
By comparative example 1 it is recognised that be added without Elymus nutans extract, to the Color of silk fabric and anti-
Bacterium performance all has considerable influence.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope that the invention discloses, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (5)
1. one kind utilizes the prodigiosin colouring method to silk fabric, it is characterised in that comprise the following steps:
A, dissolve the prodigiosin by by ethyl acetate, and add Elymus nutans extract, be then added in water, make dyeing
Solution；
B, using above-mentioned staining solution to dye silk fabric, the consumption of prodigiosin is silk fabric mass fraction
0.1-0.5%, dye bath ratio is 1: 10-20, and the pH value of regulation dye liquor is 4.0-6.0, and dyeing temperature is 60-70 DEG C, during insulation
Between be 15-25min；
After C, dyeing terminate, by fabric washing, 120 DEG C of drying 3-5min after insulation is terminated,.
The most according to claim 1 utilize the prodigiosin colouring method to silk fabric, it is characterised in that described spirit
The preparation method of bacterioruberin, comprises the following steps:
Step one: the strain of purchase is diluted, after will dilution after antibacterial coat put on plating medium constant temperature training
Support in case and cultivate；
Step 2: picking red pigment and the deeper bacterial strain of color are coated on plating medium, and by this bacterial strain the condition of 4 DEG C
Lower preservation；
Step 3: the bacterial strain screened is cultivated in seed culture medium 6-8h, the rear fermentation medium that accesses cultivates 15h；
Step 4: respectively supernatant and thalline are separated；Supernatant directly by ethyl acetate by prodigiosin from aqueous solution
In extract, extract in acidic methanol after concentrating by rotary evaporator after flocculate is first centrifugal；
Plating medium in described step one, including following main component: yeast powder 10g/L, peptone 20g/L, Fructus Vitis viniferae
Sugar 2g/L, agar powder 20g/L, constant volume to 1L, surplus is water；
The described seed culture medium in step 3, including following main component: sucrose 15g/L, yeast extract 0.5g/L, chlorination
Sodium 0.2g/L, magnesium sulfate 1g/L, constant volume to 1L, surplus is water.
The most according to claim 1 utilize the prodigiosin colouring method to silk fabric, it is characterised in that described silkworm
Silk fabrics is before use through sterilization processing.
The most according to claim 3 utilize the prodigiosin colouring method to silk fabric, it is characterised in that described kills
Bacterium processing method is: 150 DEG C of high temperature sterilize 10min.
The most according to claim 1 utilize the prodigiosin colouring method to silk fabric, it is characterised in that include following
Step:
A, dissolve the prodigiosin by by ethyl acetate, and add Elymus nutans extract, be then added in water, make dyeing
Solution；
B, using above-mentioned staining solution to dye silk fabric, the consumption of prodigiosin is silk fabric mass fraction
0.4%, dye bath ratio is 1: 15, and the pH value of regulation dye liquor is 4.0-6.0, and dyeing temperature is 65 DEG C, and temperature retention time is 20min；
After C, dyeing terminate, by fabric washing, 120 DEG C of drying 4min after insulation is terminated,；
The preparation method of described prodigiosin, comprises the following steps:
Step one: the strain of purchase is diluted, after will dilution after antibacterial coat put on plating medium constant temperature training
Support in case and cultivate；
Step 2: picking red pigment and the deeper bacterial strain of color are coated on plating medium, and by this bacterial strain the condition of 4 DEG C
Lower preservation；
Step 3: the bacterial strain screened is cultivated in seed culture medium 6h, the rear fermentation medium that accesses cultivates 15h；
Step 4: respectively supernatant and thalline are separated；Supernatant directly by ethyl acetate by prodigiosin from aqueous solution
In extract, extract in acidic methanol after concentrating by rotary evaporator after flocculate is first centrifugal；
Plating medium in described step one, including following main component: yeast powder 10g/L, peptone 20g/L, Fructus Vitis viniferae
Sugar 2g/L, agar powder 20g/L, constant volume to 1L, surplus is water；
The described seed culture medium in step 3, including following main component: sucrose 15g/L, yeast extract 0.5g/L, chlorination
Sodium 0.2g/L, magnesium sulfate 1g/L, constant volume to 1L, surplus is water；Regulation pH is 7.0-7.5；
Described silk fabric needs through sterilization processing before use, and described bactericidal treatments is: 150 DEG C of high temperature sterilizes
10min。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610534232.4A CN106192459A (en) | 2016-07-04 | 2016-07-04 | A kind of utilize the prodigiosin colouring method to silk fabric |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610534232.4A CN106192459A (en) | 2016-07-04 | 2016-07-04 | A kind of utilize the prodigiosin colouring method to silk fabric |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108411631A (en) * | 2018-04-16 | 2018-08-17 | 佛山市尚柏科技有限公司 | A kind of washable textile product inorganic agent |
| CN108720032A (en) * | 2017-04-19 | 2018-11-02 | 嘉兴学院 | A kind of natural pigment pure cellulose food and preparation method thereof |
| CN112375791A (en) * | 2020-11-26 | 2021-02-19 | 河池学院 | Preparation and dyeing method of streptomyces spectabilis haematochrome |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102493220A (en) * | 2011-12-09 | 2012-06-13 | 东华大学 | Method for dyeing acrylic fiber fabrics by utilizing bacterium dyestuff prodigiosin |
| CN102493228A (en) * | 2011-12-09 | 2012-06-13 | 东华大学 | Method for dyeing wool fabric by using bacterial dye of prodigiosin |
-
2016
- 2016-07-04 CN CN201610534232.4A patent/CN106192459A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102493220A (en) * | 2011-12-09 | 2012-06-13 | 东华大学 | Method for dyeing acrylic fiber fabrics by utilizing bacterium dyestuff prodigiosin |
| CN102493228A (en) * | 2011-12-09 | 2012-06-13 | 东华大学 | Method for dyeing wool fabric by using bacterial dye of prodigiosin |
Non-Patent Citations (1)
| Title |
|---|
| 钟绵国: "灵菌红素的合成及其染色性能研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108720032A (en) * | 2017-04-19 | 2018-11-02 | 嘉兴学院 | A kind of natural pigment pure cellulose food and preparation method thereof |
| CN108411631A (en) * | 2018-04-16 | 2018-08-17 | 佛山市尚柏科技有限公司 | A kind of washable textile product inorganic agent |
| CN112375791A (en) * | 2020-11-26 | 2021-02-19 | 河池学院 | Preparation and dyeing method of streptomyces spectabilis haematochrome |
| CN112375791B (en) * | 2020-11-26 | 2022-04-22 | 河池学院 | Preparation and dyeing method of streptomyces spectabilis haematochrome |
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