CN106190860A - A kind of bolete culture medium adding Nano bacteria cellulose and the preparation method of bolete liquid spawn - Google Patents

Abstract

The invention discloses a kind of bolete culture medium adding Nano bacteria cellulose, including following parts by weight of component: Corn Porous Starch 98 100, yeast leaching powder 89 90, glucose 36 38, edible peanut oil 2.9 3, sodium bicarbonate 1.5 1.6, yolk 2.1 2.2, poly-aspartate 2.2 2.3, Folium Ligustri pubescentis oil 1.9 2, ethylmaltol 1.4 1.5, nano-scaled electric stone powder 4.6 4.8, Nano bacteria cellulose 6.8 7 etc..The present invention uses Corn Porous Starch, yeast leaching powder to coordinate nutritional supplements matter for bolete liquid spawn, nutritious, with Nano bacteria cellulose, Corn Porous Starch is carried out modification, on the one hand the material composition of culture medium is added, on the other hand improving the uniformity of culture medium, stability and space structure characteristic, good formation and growth promoter for bolete liquid spawn mycelium pellet provide guarantee.

Description

A kind of bolete culture medium adding Nano bacteria cellulose and bolete liquid bacteria The preparation method planted

Technical field

The present invention relates to liquid spawn technical field, particularly relate to a kind of bolete adding Nano bacteria cellulose and cultivate Base and the preparation method of bolete liquid spawn.

Background technology

In bolete production process, liquid spawn has the advantage that solid spawn is incomparable, and liquid spawn has life The product cycle is short, and cell age is short and consistent, and purity is high, energetic, inoculates simple and efficient, easily carries out batch production, scale, standard metaplasia The advantages such as product.Now, increasing people is research and use liquid spawn, and the popularization of liquid spawn and use are also real Existing edible fungus industrial, scale, standardized inexorable trend.But liquid spawn there is also defect and the deficiency of self: 1, the many fast growths of liquid spawn germination point, but penetration power is not strong, and compost is crossed thick mycelia and is difficult to have thorough grasp compost, mycelia Will be along compost surface fast-growth, the internal hyphae length of compost is little, the problem having had a strong impact on strain yield and quality； Although 2, conventional liquid spawn solves some problems that solid spawn exists, can substantially shorten cultivation period, strengthen strain Vigor and resistance, reduction strain pollution rate, strain quality regularity is improved, but still suffer from the liquid obtained that such as ferments The deficiencies such as in strain the physical characteristic such as the density of fungus ball, size, the uniformity is less desirable, if the method sheared with stirring will Fungus ball is smashed, and the strain uniformity obtained can be more preferable, but, high-speed stirred is pulverized and strain is injured pole by the shearing force of making beating Greatly, the quality impact on strain is overall is the most serious.Exploitation is evenly distributed and has the high-quality liquid of smaller particle mycelium pellet Body strain is significant.Publication number CN105309194A " edible fungi particle liquid strain production new technology " provides one Mycelium pellet particle diameter 0.5 edible fungi particle liquid strain, the increase edible fungi liquid strain culture fluid ventilation of employing, make Ventilation is suitable at 1:0.6 0.9V/V min, and in air blow tank, device agitator makes culture fluid eddy motion simultaneously Extending the technology operation method of the moving line of bubble, the method for this production particle liquid strain is to change from production technology Entering, liquid spawn culture medium aspect does not improves, and implements technical difficulty and requires height；Publication number CN105130516A " molecular level biological medium manufacture method " refer to molecular level biological medium nutritional labeling and can the most comprehensively be absorbed, Promote the growth of liquid spawn, but do not mention the molecular level biological medium shadow to liquid spawn mycelium pellet diameter Ring.Development is a kind of improves edible fungus species product based on liquid spawn culture medium composition of raw material improvement liquid spawn mycelium pellet diameter Amount and the technology of quality, have great importance.

Nano bacteria cellulose is one of abundant renewable product of nature, and Nano bacteria cellulose is cellulose Physics minimum structural unit, refers to the fiber between diameter 1-100nm, Nano bacteria cellulose light weight, and good biocompatibility can Degraded, renewable, reactivity is high, and it is high to have Young's modulus, and the degree of polymerization is high, and cleanliness factor is high, and intensity is high, and specific surface area is big Advantage.Nano bacteria cellulose is made as edible fungus liquid culture growth medium raw material, the mycelia to edible fungi liquid strain Ball distribution and diameter tool have a certain impact, and then play the effect improving edible fungus species quality.

Summary of the invention

The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of Hepar Bovis seu Bubali adding Nano bacteria cellulose Bacterium culture medium and bolete liquid spawn preparation method.

The present invention is achieved by the following technical solutions:

A kind of bolete culture medium adding Nano bacteria cellulose, including following parts by weight of component: Corn Porous Starch 98- 100, yeast soaks powder 89-90, glucose 36-38, edible peanut oil 2.9-3, sodium bicarbonate 1.5-1.6, yolk 2.1-2.2, gathers Aspartic acid 2.2-2.3, Folium Ligustri pubescentis oil 1.9-2, ethylmaltol 1.4-1.5, potassium sorbate 1.1-1.2, Gypsum Fibrosum 1.1-1.2, mistake Calcium phosphate 1.0-1.2, KH2PO40.7-0.8, MgSO40.6-0.7, nano-scaled electric stone powder 4.6-4.8, Nano bacteria cellulose 6.8-7, appropriate water.

A kind of method using above-mentioned culture medium to prepare bolete liquid spawn, comprises the following steps:

(1), nano-scaled electric stone powder and water being mixed according to 0.1g/L, energising excites that to prepare sterilized water standby；By stone Gypsum Fibrosum, mistake Calcium phosphate, the total amount of KH2PO4, MgSO4 and sterilized water are blended in 30 DEG C of stirring and dissolving by 1g/L and obtain solution one；By edible peanut Oil, sodium bicarbonate, yolk, poly-aspartate, Folium Ligustri pubescentis oil, ethylmaltol, the total amount of potassium sorbate and sterilized water are pressed 4g/L and are mixed Close stirring and dissolving and obtain solution two；By Corn Porous Starch, the mixing of half Nano bacteria cellulose, stir little fire and bake 30min, Modified porous corn starch, presses 300g/L mixing by modified porous corn starch, yeast leaching powder, the total amount of glucose and sterilized water Stirring, and carry out pasteurization enzyme denaturing 10min carrying out 98 DEG C, obtain solution three standby；

(2), by solution three keep 20 DEG C, solution two keep 25 DEG C, solution one keep 30 DEG C, first solution one is joined solution two Middle mixing and stirring obtains complex liquid, obtains nutritional solution this complex liquid joins mixing and stirring in solution three, and adjusts PH, hereafter carries out energising and excites process 30min to obtain fluid medium standby this nutritional solution；

(3), by Hepar Bovis seu Bubali starter kind being first inoculated in gnotobasis on the fluid medium of half and carry out liquid fermentation, ferment bar Part is pH5.3-5.5, inoculum concentration 11%, temperature 20-21 DEG C, shaking speed 100r/min, cultivate 24-25h, obtain bolete liquid Strain one；

(4), fluid medium and the residue half Nano bacteria cellulose mix homogeneously of half will be remained, be placed into aseptic training Support high temperature sterilizing again in bottle, obtain complex medium, the bolete liquid spawn one that step (3) is obtained by 14% inoculum concentration Gnotobasis is inoculated in this culture bottle, temperature 19-20 DEG C, cultivates 30-32h and get final product.

The invention have the advantage that

The present invention uses Corn Porous Starch, yeast leaching powder to coordinate nutritional supplements matter yolk, poly-aspartate, Folium Ligustri pubescentis oil, second Base maltol and trace substance calcium potassium phosphorus magnesium prepare bolete liquid spawn, nutritious, meanwhile, and the yolk of addition, poly-sky Winter propylhomoserin, Folium Ligustri pubescentis oil, ethylmaltol have the effect improving bolete liquid spawn environmental stress ability, use bacteria nano Cellulose has carried out modification to Corn Porous Starch, on the one hand adds the material composition of culture medium, on the other hand improves The uniformity of culture medium, stability and space structure characteristic, for bolete liquid spawn mycelium pellet good formation and Growth promoter provides guarantee；Bolete liquid spawn mycelium pellet distribution prepared by basal culture medium, is sized to meet later stage Entity growth promoter demand is without further mechanical activation comminution, high financial profit.

Detailed description of the invention

A kind of bolete culture medium adding Nano bacteria cellulose, including following parts by weight of component: Corn Porous Starch 98, yeast leaching powder 89, glucose 36, edible peanut oil 2.9, sodium bicarbonate 1.5, yolk 2.1, poly-aspartate 2.2, Folium Ligustri pubescentis oil 1.9, ethylmaltol 1.4, potassium sorbate 1.1, Gypsum Fibrosum 1.1, calcium superphosphate 1.0, KH2PO40.7, MgSO40.6, nanoscale electricity Gas stone powder 4.6, Nano bacteria cellulose 6.8, appropriate water.

A kind of method that above-mentioned culture medium prepares bolete liquid spawn, comprises the following steps:

(1), nano-scaled electric stone powder and water being mixed according to 0.1g/L, energising excites that to prepare sterilized water standby；By stone Gypsum Fibrosum, mistake Calcium phosphate, the total amount of KH2PO4, MgSO4 and sterilized water are blended in 30 DEG C of stirring and dissolving by 1g/L and obtain solution one；By edible peanut Oil, sodium bicarbonate, yolk, poly-aspartate, Folium Ligustri pubescentis oil, ethylmaltol, the total amount of potassium sorbate and sterilized water are pressed 4g/L and are mixed Close stirring and dissolving and obtain solution two；By Corn Porous Starch, the mixing of half Nano bacteria cellulose, stir little fire and bake 30min, Modified porous corn starch, presses 300g/L mixing by modified porous corn starch, yeast leaching powder, the total amount of glucose and sterilized water Stirring, and carry out pasteurization enzyme denaturing 10min carrying out 98 DEG C, obtain solution three standby；

(2), by solution three keep 20 DEG C, solution two keep 25 DEG C, solution one keep 30 DEG C, first solution one is joined solution two Middle mixing and stirring obtains complex liquid, obtains nutritional solution this complex liquid joins mixing and stirring in solution three, and adjusts PH, hereafter carries out energising and excites process 30min to obtain fluid medium standby this nutritional solution；

(3), by Hepar Bovis seu Bubali starter kind being first inoculated in gnotobasis on the fluid medium of half and carry out liquid fermentation, ferment bar Part is pH5.3, inoculum concentration 11%, temperature 20 DEG C, shaking speed 100r/min, cultivate 24h, obtain bolete liquid spawn one；

(4), fluid medium and the residue half Nano bacteria cellulose mix homogeneously of half will be remained, be placed into aseptic training Support high temperature sterilizing again in bottle, obtain complex medium, the bolete liquid spawn one that step (3) is obtained by 14% inoculum concentration Gnotobasis is inoculated in this culture bottle, temperature 19 DEG C, cultivates 30h and get final product.

Claims (2)

1. the bolete culture medium adding Nano bacteria cellulose, it is characterised in that include following parts by weight of component: porous Corn starch 98-100, yeast leaching powder 89-90, glucose 36-38, edible peanut oil 2.9-3, sodium bicarbonate 1.5-1.6, yolk 2.1-2.2, poly-aspartate 2.2-2.3, Folium Ligustri pubescentis oil 1.9-2, ethylmaltol 1.4-1.5, potassium sorbate 1.1-1.2, Gypsum Fibrosum 1.1-1.2, calcium superphosphate 1.0-1.2, KH2PO40.7-0.8, MgSO40.6-0.7, nano-scaled electric stone powder 4.6-4.8, antibacterial Nano-cellulose 6.8-7, appropriate water.

2. one kind uses the method that the culture medium described in claim 1 prepares bolete liquid spawn, it is characterised in that include with Lower step:

(1), nano-scaled electric stone powder and water being mixed according to 0.1g/L, energising excites that to prepare sterilized water standby；By stone Gypsum Fibrosum, mistake Calcium phosphate, the total amount of KH2PO4, MgSO4 and sterilized water are blended in 30 DEG C of stirring and dissolving by 1g/L and obtain solution one；By edible peanut Oil, sodium bicarbonate, yolk, poly-aspartate, Folium Ligustri pubescentis oil, ethylmaltol, the total amount of potassium sorbate and sterilized water are pressed 4g/L and are mixed Close stirring and dissolving and obtain solution two；By Corn Porous Starch, the mixing of half Nano bacteria cellulose, stir little fire and bake 30min, Modified porous corn starch, presses 300g/L mixing by modified porous corn starch, yeast leaching powder, the total amount of glucose and sterilized water Stirring, and carry out pasteurization enzyme denaturing 10min carrying out 98 DEG C, obtain solution three standby；

(2), by solution three keep 20 DEG C, solution two keep 25 DEG C, solution one keep 30 DEG C, first solution one is joined solution two Middle mixing and stirring obtains complex liquid, obtains nutritional solution this complex liquid joins mixing and stirring in solution three, and adjusts PH, hereafter carries out energising and excites process 30min to obtain fluid medium standby this nutritional solution；

(3), by Hepar Bovis seu Bubali starter kind being first inoculated in gnotobasis on the fluid medium of half and carry out liquid fermentation, ferment bar Part is pH5.3-5.5, inoculum concentration 11%, temperature 20-21 DEG C, shaking speed 100r/min, cultivate 24-25h, obtain bolete liquid Strain one；

(4), fluid medium and the residue half Nano bacteria cellulose mix homogeneously of half will be remained, be placed into aseptic training Support high temperature sterilizing again in bottle, obtain complex medium, the bolete liquid spawn one that step (3) is obtained by 14% inoculum concentration Gnotobasis is inoculated in this culture bottle, temperature 19-20 DEG C, cultivates 30-32h and get final product.