CN106190818A - Full automatic microorganism is identified and drug sensitive test system - Google Patents
Full automatic microorganism is identified and drug sensitive test system Download PDFInfo
- Publication number
- CN106190818A CN106190818A CN201610598928.3A CN201610598928A CN106190818A CN 106190818 A CN106190818 A CN 106190818A CN 201610598928 A CN201610598928 A CN 201610598928A CN 106190818 A CN106190818 A CN 106190818A
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- China
- Prior art keywords
- card
- storehouse
- test
- full automatic
- drug sensitive
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Abstract
nullThe invention belongs to microbiological test equipment technical field，Particularly relate to a kind of full automatic microorganism identify and drug sensitive test system，Gas-tight silo is filled including card、Card loading pocket、Card hatches storehouse and card unloading storehouse，And optical reader、Chromacoder and data processing equipment，Wherein card is filled on gas-tight silo equipped with vacuum extractor，Card is filled and is provided with connecting tube between gas-tight silo and vacuum extractor，Equipped with valve in connecting tube，Card is filled and is respectively equipped with card holder inlet/outlet in the import and export of gas-tight silo，Card holder transporter it is provided with in card loading pocket、Barcode recognizer、Heat-sealing machine、Card loader and card holder emptier，Card is provided with rotatable card and hatches frame and heating unit in hatching storehouse，Card unloading is provided with card emptier in storehouse，Optical reader includes card shifter and optical reader，Chromacoder is that optical signalling turns digital signal device，Data processing equipment includes main frame and display.Work efficiency of the present invention is high, security performance is good, and qualification result is fast, accuracy is high.
Description
Technical field
The invention belongs to microbiological test equipment technical field, particularly relate to a kind of full automatic microorganism and identify and susceptibility
Pilot system.
Background technology
Traditional microbial identification and drug sensitive test are by hand or mechanical arm is loaded, last after hatching certain time
Unification end-point method carries out colorimetric qualification.This sample-adding and qualification mode have the drawback that: 1, feed rate is slow, work effect
Rate is low；Environment is easily caused biological dirty by the microbial aerosol 2, produced during sample-adding and the bread board accidentally spilt
Dye, constitutes a threat to staff's health；3, end-point method colorimetric is according to the work of staff all bread boards hatched
Breath time and custom is unified carries out colorimetric to the last moment so that the major part sample accused of can transmitting messages in advance is postponed artificially
Get off, be easily delayed the state of an illness diagnosis and treatment of patient.
Chinese utility model patent CN201999938U discloses a kind of automatic filling test card for bacteria drug sensitivity test, structure
Simply, it is not necessary to complex instrument, drug sensitive test can be carried out automatically to fill.Its design principle is (i.e. to test based on bread board
Card) forvacuum, finally that it is airtight.Specifically used process, i.e. carries out drug sensitive test when automatically filling, due to its sample introduction tube head
Being to be provided with water soluble plastic film, this film dissolves when contact liq sample, and liquid sample is under atmospheric pressure
Automatically into bread board (i.e. testing card).This sample loading alternative has the drawback that: 1, bread board (i.e. testing card) preservation condition is severe
Carving, sealing system is easily leaked gas, and causes vacuum degree deficiency, occurs that sample-adding is not fully filled situation about maybe can not be loaded；2, it is only suitable on a small quantity
The single sample-adding of specimen, it is impossible to carry out batch and be loaded.
Summary of the invention
The technical problem to be solved in the present invention overcomes above-mentioned the deficiencies in the prior art exactly, and provides a kind of work efficiency
Height, security performance are good, and the full automatic microorganism that qualification result is fast, accuracy is high is identified and drug sensitive test system.
For solving above-mentioned technical problem, the technical solution used in the present invention is:
A kind of full automatic microorganism of design is identified and drug sensitive test system, fills gas-tight silo, card loading pocket, card including card
Hatching storehouse and card unloading storehouse, and optical reader, chromacoder and data processing equipment, wherein card is filled close
Equipped with vacuum extractor on envelope storehouse, card is filled and is provided with connecting tube, equipped with valve in connecting tube between gas-tight silo and vacuum extractor
Door, card is filled and is respectively equipped with card holder inlet/outlet in the import and export of gas-tight silo；
Card holder transporter, barcode recognizer, heat-sealing machine, card loader and card holder unloading it is provided with in described card loading pocket
Device, card fill gas-tight silo outlet with card loading pocket set by entrance be connected；
Described card is provided with rotatable card and hatches frame and heating unit in hatching storehouse, card is hatched storehouse and is provided with card import and export
Respectively with card loading pocket set by outlet and card unload entrance set by storehouse and connect；The unloading of described card is provided with card unloading in storehouse
Device；
Described optical reader includes card shifter and optical reader, and is installed in card loading pocket or card unloading storehouse
In；Described chromacoder is that optical signalling turns digital signal device；Described data processing equipment includes main frame and display.
Preferably, identify at above-mentioned full automatic microorganism and in drug sensitive test system, also include the card carriages matched
The test tube filling liquid microorganism specimen filled on and and test card, test card is circumscribed with sample feeding pipe, sample introduction
Pipe end is inserted in test tube；Bar code is all posted in card carriages and test card.
Preferably, in the qualification of above-mentioned full automatic microorganism and drug sensitive test system, described test card includes plastic plate
And the microchannel that on plastic plate, set test microvia connects with test microvia, described test microvia is matrix of holes, plastic plate
Two surfaces are coated with transparent membrane and form transparent chamber with matrix of holes, and the described external sample feeding pipe of test card, sample feeding pipe passes through side
Face interface successively with plastic sheet surface set by microchannel and test microvia connect.
Preferably, identify and in drug sensitive test system at above-mentioned full automatic microorganism, on described plastic plate, in test microvia
Edge is provided with the secondary orifices being connected；Coated reaction substrate or antibiotic it is preinstalled with in test microvia.
Preferably, identifying and in drug sensitive test system at above-mentioned full automatic microorganism, described card holder inlet/outlet is provided with certainly
Dynamic opener.
Preferably, identify at above-mentioned full automatic microorganism and in drug sensitive test system, be respectively relative to card holder transporter, card
Sheet loader, card holder emptier, card shifter and card emptier, corresponding coupling is provided with track；It is provided with bottom card carriages
Draw-in groove, fastens with respective rail.
Preferably, in the qualification of above-mentioned full automatic microorganism and drug sensitive test system, it is axis water that described card hatches frame
The flat circular cylinder structure arranged, is radially provided with multiple card groove along drum surface.
Preferably, in the qualification of above-mentioned full automatic microorganism and drug sensitive test system, described vacuum means is set to vacuum pump；
Described valve is air door.
Preferably, in the qualification of above-mentioned full automatic microorganism and drug sensitive test system, described chromacoder is electric charge
Coupled apparatus, and be arranged in main frame.
Preferably, identifying and in drug sensitive test system at above-mentioned full automatic microorganism, described card fills gas-tight silo, card
Loading pocket, card hatch storehouse and card unloading storehouse is that a word is arranged side by side.
Technical solution of the present invention provides the benefit that:
1, the present invention is by being placed in test card, test tube and bracket in filling gas-tight silo, and rear employing vacuum extractor will fill storehouse
Gradually extract out with the air in card, until it reaches near vacuum.Close vacuum equipment, slowly open valve, fill the pressure in storehouse
Power is progressively recovered, and in sample tube, liquid sample can enter in card test microvia due to the effect of atmospheric pressure, reaches card
The purpose that sample is filled.Card can single be filled, it is also possible to multiple be filled with simultaneously.Relative to existing mode, sample-adding effect
Rate significantly improves；And be the real-time evacuation of qualification process rather than forvacuum, do not have and cause vacuum because of gas leakage
Deficiency, occurs that sample-adding is not fully filled situation about maybe can not be loaded.
2, frame and heating unit, card shifter and optical reader, and signal are hatched owing to being provided with rotatable card
Conversion equipment and the setting of data processing equipment, can make card constantly be reversed rotation mixing and carry out constant temperature hatching on frame
Cultivating, along with the increase of micro organism quantity, in card test microvia, the most coated substrate can be sent out due to the effect of microorganism
Raw color or the change of turbidity.The most at regular intervals, card will be removed by card shifter, by optical reader to card
Upper each test microvia scans reading subtly, is sent back to hatch frame the most again and continues to cultivate.Then second card sector-meeting quilt
Similarly remove and read.And so on, hatch all cards on frame and all can periodically be removed and carry out optical reading.
The optical signalling read can be converted into digital signal in time and be exported, main frame dynamically analyze, can
With quickly draw microbial identification result or drug sensitive test result.Thus, the mode of this dynamic Testing and appraisal, relative to tradition
The method of end point determination, its qualification result is fast, accuracy is high.
3, multiple test card and corresponding can be placed owing to test card is provided with on matrix of holes, and each bracket
Test tube, coordinates Vacuuming structure, can complete batch and be loaded, further increase sample-adding efficiency, also a saving in gas-tight silo
Consumables cost and save the consumption of liquid reagent；The purpose that arranges of secondary orifices is to obtain enough vacuum pressure differential, and
The bubble that microorganism growth process produces can also enter into this hole, reduces the error that optics judges, improves the standard of test result
Really property.Hatch frame drum surface and be provided with multiple card slot radially, multiple cards can be carried out clamping, to multiple cards simultaneously
Sheet carries out constant temperature culture, and periodically removes and carry out optical reading, then further increase work efficiency.
4 additionally, the links identified, i.e. card is filled, card loads, card is hatched, optical reading and card,
Unloading of card holder etc., is all to carry out in the environment of airtight, has stopped environment is caused biological pollution, it is ensured that staff
Healthy.Thus, relative to work on hand mode, this system uses process safety performance higher.
Accompanying drawing explanation
Below in conjunction with the accompanying drawings the detailed description of the invention of the present invention is described in further detail, wherein:
Fig. 1 is one of structural representation of full automatic microorganism of the present invention qualification and drug sensitive test system；
Fig. 2 is the card carriages shown in Fig. 1 and test tube, the assembling structural representation of test card；
Fig. 3 is the structural representation of the test card shown in Fig. 2；
Fig. 4 is the A-A sectional structure schematic diagram shown in Fig. 3；
Fig. 5 is the side-looking structural representation that the card shown in Fig. 1 hatches storehouse；
Fig. 6 is the B-B sectional structure schematic diagram shown in Fig. 1；
Sequence number in figure: 1, gas-tight silo filled by card, 2, card loading pocket, 3, card hatch storehouse, 4, card unloading storehouse, 5, vacuum
Pump, 6, connecting tube, 7, air door, 8, card holder inlet/outlet, 9, card holder transporter, 10, barcode recognizer, 11, heat-sealing machine,
12, card loader, 13, card holder emptier, 14, card emptier, 15, optical reader, 16, main frame and display, 17, survey
Examination card, 18, sample feeding pipe, 19, test tube, 20, card carriages, 21, bar code, 22, plastic plate, 23, test microvia, 24, micro-logical
Road, 25, secondary orifices, 26, card hatch frame, 27, heating unit, 28, card import and export, 29, card groove, 30, track, 31, thoroughly
Bright thin film.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the present invention, the technical scheme in the embodiment of the present invention is carried out the most complete description, aobvious
The most described embodiment is only the present invention schematic part detailed description of the invention, is not limited to the scope of the present invention,
Equivalent variations that any those skilled in the art is made on the premise of without departing from present inventive concept and principle and amendment, all
The scope of protection of the invention should be belonged to.
Seeing Fig. 1-6, in figure, full automatic microorganism of the present invention is identified and drug sensitive test system, arranges including word order
Card fill gas-tight silo 1, card loading pocket 2, card hatch storehouse 3 and card unloading storehouse 4, and optical reader, signal
Conversion equipment and data processing equipment, also include the card carriages matched and on filled fill liquid microorganism specimen
Test tube and test card.
Seeing Fig. 1, in figure, card is filled equipped with vacuum pump 5 on gas-tight silo, and card is filled and set between gas-tight silo and vacuum pump
Having connecting tube 6, equipped with air door 7 in connecting tube, card is filled and is respectively equipped with card holder inlet/outlet in the import and export of gas-tight silo；Card
Frame inlet/outlet includes that card holder enters door and card holder removal door 8 is provided with automatic-opening mechanism.It is provided with card holder in card loading pocket to turn
Fortune device 9, barcode recognizer 10, heat-sealing machine 11, card loader 12 and card holder emptier 13, card fill gas-tight silo outlet with
Entrance set by card loading pocket is connected.Card unloading is provided with card emptier 14 in storehouse；Optical reader includes that card moves
Dynamic device and optical reader 15, and be installed in card unloading storehouse, wherein card shifter can use card emptier；Data
Processing means includes main frame and display 16；Chromacoder is that optical signalling turns digital signal device, specially electric charge coupling
Clutch part, and be arranged in main frame.
Seeing Fig. 2-4, in figure, test card 17 is circumscribed with sample feeding pipe 18, sample feeding pipe end is inserted in test tube 19；Card
Bar code 21 is all posted on sheet bracket 20 and test card.Test card includes that test set on plastic plate 22 and plastic plate is micro-
The microchannel 24 that hole 23 connects with test microvia, test microvia is matrix of holes, and plastic plate two surface is coated with transparent membrane 31
With matrix of holes form transparent chamber, test block external sample feeding pipe, sample feeding pipe by side face port successively with plastic sheet surface institute
If microchannel connects with test microvia.On plastic plate, it is provided with the secondary orifices 25 being connected in test microvia edge；In test microvia
It is preinstalled with coated substrate or antibiotic (not shown).
Seeing Fig. 5-6, in figure, card is provided with rotatable card and hatches frame 26 and heating unit 27 in hatching storehouse, card is incubated
Educate storehouse be provided with card import and export 28 respectively with card loading pocket set by outlet and card unload entrance set by storehouse and connect；Described card
It is the circular cylinder structure that axis horizontal is arranged that sheet hatches frame, is radially provided with multiple card groove 29 along drum surface.
Further, see Fig. 1, in figure, be respectively relative to card holder transporter, card loader, card holder emptier, card
Shifter and card emptier, corresponding coupling is provided with track 30；It is provided with draw-in groove bottom card carriages, fastens with respective rail.
Specific works process approximately as:
The test tube and test card that fill liquid microorganism specimen are loaded onto card carriages simultaneously, and the sample feeding pipe of card inserts examination
In pipe, then bracket is inserted on filling gas-tight silo inner orbit.Start vacuum machine, fill the air in gas-tight silo and card gradually
Can be drawn out of, until it reaches near vacuum.Closing vacuum pump, slowly open air door, the pressure filling gas-tight silo is the most extensive
Multiple, in sample tube, liquid sample can enter in card test microvia due to the effect of atmospheric pressure, reaches card sample and fills
Purpose.Card can single be filled, it is also possible to multiple be filled with simultaneously.
After card is filled, the card holder removal door filling gas-tight silo automatically opens up, and test card bracket is in transporter effect
Under transfer on the track in loading pocket, bar code scanner respectively card holder and card are carried out classifying and numbering.Afterwards, heat seal
Machine starts, and is blocked close to the part of card body by suction pipe, is broken by suction pipe cross section envelope due to the effect of heat seal while blocking
Close.Then card is hatched frame by loader loading one by one, and test card bracket is from the unloaded release of loading pocket.
Card is constantly reversed rotation mixing and carries out constant temperature culture hatching on frame, along with the increase of micro organism quantity,
In card micropore, the most coated reaction substrate can occur the change of color or turbidity due to the effect of microorganism.Every necessarily
Time, card will be removed by card shifter, optical reader micropore each on card is scanned reading subtly,
It is sent back to hatch frame the most again continue to cultivate.Then second card sector-meeting is similarly removed and is read.And so on, hatch frame
On all cards all can periodically be removed and be carried out optical reading.The optical signalling read can be converted in time
Digital signal and be exported, computer dynamically analyze, quickly can draw qualification result.The card of each is identified
Result out after, card will unloaded device release, fall into unloading storehouse.
Thus, the whole work process of this system, work efficiency is high, security performance is good, and qualification result is fast, accuracy is high,
Enforcement easy to spread.
In this specification, each embodiment uses the mode gone forward one by one to be described, and what each embodiment stressed is and it
The difference of its embodiment, between each embodiment, identical similar part is cross-referenced.
Described above to disclosed embodiment, makes those skilled in the art be capable of or uses the present invention.To these
The many places amendment of embodiment is apparent to those skilled in the art, and generic principles defined herein can be
On the premise of spirit or scope without departing from invention, realize in other embodiments.Such as the relative position in each storehouse is according to reality
Border situation can freely adjust, and as compact conformation, card unloading storehouse may be mounted at lower section or the side of card loading pocket
Lower section, notably card unloading storehouse shares storehouse etc. with card loading pocket, belongs to invention which is intended to be protected.Cause
This, the present invention will be not restricted to these embodiments illustrated herein, and be to fit to and principles disclosed herein and features of novelty
Consistent widest range.
Claims (10)
1. full automatic microorganism is identified and a drug sensitive test system, it is characterized in that: include that gas-tight silo filled by card, card loads
Storehouse and card unloading storehouse, and optical reader, chromacoder and data processing equipment are hatched in storehouse, card, wherein block
Sheet is filled equipped with vacuum extractor on gas-tight silo, and card is filled and is provided with connecting tube, connecting tube between gas-tight silo and vacuum extractor
On equipped with valve, card is filled and is respectively equipped with card holder inlet/outlet in the import and export of gas-tight silo；
Card holder transporter, barcode recognizer, heat-sealing machine, card loader and card holder unloading it is provided with in described card loading pocket
Device, card fill gas-tight silo outlet with card loading pocket set by entrance be connected；
Described card is provided with rotatable card and hatches frame and heating unit in hatching storehouse, card is hatched storehouse and is provided with card import and export
Respectively with card loading pocket set by outlet and card unload entrance set by storehouse and connect；The unloading of described card is provided with card unloading in storehouse
Device；
Described optical reader includes card shifter and optical reader, and is installed in card loading pocket or card unloading storehouse
In；Described chromacoder is that optical signalling turns digital signal device；Described data processing equipment includes main frame and display.
Full automatic microorganism the most according to claim 1 is identified and drug sensitive test system, it is characterized in that: also include matching
Card carriages and on the test tube filling liquid microorganism specimen that filled and test card, test card is circumscribed with into
Sample pipe, sample feeding pipe end is inserted in test tube；Bar code is all posted in card carriages and test card.
Full automatic microorganism the most according to claim 2 is identified and drug sensitive test system, it is characterized in that: described test card
The microchannel connected with test microvia including test microvia set on plastic plate and plastic plate, described test microvia is series
Through hole, plastic plate two surface is coated with transparent membrane and forms transparent chamber, the described external sample feeding pipe of test card with matrix of holes, enters
Sample pipe by side face port successively with plastic sheet surface set by microchannel and test microvia connect.
Full automatic microorganism the most according to claim 3 is identified and drug sensitive test system, it is characterized in that: described plastic plate
Above, the secondary orifices being connected it is provided with in test microvia edge；Coated reaction substrate or antibiotic it is preinstalled with in test microvia.
5. identify and drug sensitive test system according to the full automatic microorganism described in any one of claim 2 to 4, it is characterized in that: institute
State card holder inlet/outlet and be provided with automatic-opening mechanism.
Full automatic microorganism the most according to claim 5 is identified and drug sensitive test system, it is characterized in that: be respectively relative to card
Frame transporter, card loader, card holder emptier, card shifter and card emptier, corresponding coupling is provided with track；Card holder
It is provided with draw-in groove bottom frame, fastens with respective rail.
Full automatic microorganism the most according to claim 6 is identified and drug sensitive test system, it is characterized in that: described card is hatched
Frame is the circular cylinder structure that axis horizontal is arranged, and is radially provided with multiple card groove along drum surface.
Full automatic microorganism the most according to claim 6 is identified and drug sensitive test system, it is characterized in that: described vacuum means
It is set to vacuum pump；Described valve is air door.
Full automatic microorganism the most according to claim 6 is identified and drug sensitive test system, it is characterized in that: described signal is changed
Device is charge-coupled image sensor, and is arranged in main frame.
Full automatic microorganism the most according to claim 6 is identified and drug sensitive test system, it is characterized in that: described card is filled out
Fill gas-tight silo, card loading pocket, card hatches storehouse and card unloading storehouse is that a word is arranged side by side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610598928.3A CN106190818A (en) | 2016-07-27 | 2016-07-27 | Full automatic microorganism is identified and drug sensitive test system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610598928.3A CN106190818A (en) | 2016-07-27 | 2016-07-27 | Full automatic microorganism is identified and drug sensitive test system |
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| Publication Number | Publication Date |
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| CN106190818A true CN106190818A (en) | 2016-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610598928.3A Pending CN106190818A (en) | 2016-07-27 | 2016-07-27 | Full automatic microorganism is identified and drug sensitive test system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226555A (en) * | 2018-03-16 | 2018-06-29 | 李涛 | The quick sample adding device of clinical samples and method |
| CN108226555B (en) * | 2018-03-16 | 2024-05-03 | 李涛 | Rapid sample adding device and method for clinical samples |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226555A (en) * | 2018-03-16 | 2018-06-29 | 李涛 | The quick sample adding device of clinical samples and method |
| CN108226555B (en) * | 2018-03-16 | 2024-05-03 | 李涛 | Rapid sample adding device and method for clinical samples |
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| PB01 | Publication | ||
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Application publication date: 20161207 |