CN2294473Y

CN2294473Y - Quick medicine sensitive detector determined by bacteria

Info

Publication number: CN2294473Y
Application number: CN 96223915
Authority: CN
Inventors: 王礼文
Original assignee: Jiangsu Provincial Hospital of Chinese Medicine
Current assignee: Jiangsu Provincial Hospital of Chinese Medicine
Priority date: 1996-10-23
Filing date: 1996-10-23
Publication date: 1998-10-14
Anticipated expiration: 2006-10-23

Abstract

The utility model relates to a quick medicine-sensitive detector for identifying bacteria, which is composed of a biochemical and medicine-sensitive plate, and a seal sleeve which is sheathed out of the biochemical and medicine-sensitive plate, wherein, the biochemical and medicine-sensitive plate is provided with a plurality of chromogenic holes which are respectively communicated with liquid inlet grooves; the seal sleeve is a transparent flat box, one end of which is open; the seal sleeve is in close transition fit with the biochemical and medicine-sensitive plate. The utility model is connected with a vacuum sample injector. The design use colorimetry to carry out determination with easy and quick operation, and the utility model can effectively solve the requirement of the identification of anaerobic bacteria and the determination of bacteria MIC in medical researches and clinical work.

Description

Quick bacteria identification and susceptibility detecting device

The utility model relates to a kind of quick bacteria identification and susceptibility detecting device that medically uses.

In medical research or clinical practice, correctly identify bacterium and select effective microbiotic and dosage, most important to the treatment of infectious diseases, and this selection need be based upon on the basis of " microbiotic minimum inhibitory concentration " (being MIC) mensuration.Traditional anaerobic bacteria evaluation and bacterium MIC assay method are cumbersome, and required time is long, can not adapt to requirements of one's work such as clinical rescue.When the Murry of the U.S. had proposed to utilize bacterial growth to be suppressed, the characteristics that its oxygen consumption reduces were with the partial pressure of oxygen in the blood gas analyzer mensuration nutrient solution, the scheme of fast measuring MIC.But the blood oxygen analysis instrument involves great expense, and practical operation is cumbersome, and mensuration expense height is so be unsuitable for applying.

The utility model will provide a kind of quick bacteria identification and susceptibility detecting device of new construction, this utensil should be able to provide the subenvironment of a starvation, utilization suppresses degree by the partial pressure of oxygen reacting bacteria and anaerobion need be cooked mensuration work in the characteristics of anaerobic or low-oxygen environment growth, this tools for measurement structure should be simpler, be easy to use, and cost is low, and use cost is also low.In addition, it also can be used for the observation of anaerobism and the multiple biochemical reaction of double anaerobion, and solves the needs that quick bacteria is identified and MIC measures in medical research and the clinical position.

The scheme of finishing the foregoing invention task is as follows; Quick bacteria identification and susceptibility detecting device, constitute with the airtight cover that is enclosed within its outside by biochemistry and drug sensitive plate, biochemistry and drug sensitive plate are platelets, offer several colour developing holes on it, there is lead channel to communicate with each colour developing hole respectively, airtight cover is the transparent flat box of one one end opening, and its inside dimension and biochemistry and drug sensitive plate are made transition fit (being slidingly matched) closely.Be that drug sensitive plate can freely insert in the airtight cover, generally can be coated with during insertion, be connected use, do not have the space between the two, form vacuum seal to a certain degree with vacuum pump with vacuum grease.Biochemistry and drug sensitive plate and sealing shroud can be made with the stable material of chemical property such as plastics or glass, and biochemistry and drug sensitive plate generally can be made into rectangle, but other shape that can cooperate with sealing shroud also can.A kind of prioritization scheme is: add transparent plastic film on the surface of biochemistry and drug sensitive plate.This scheme can increase its impermeability.Concrete grammar is to load onto transparent plastic film earlier in the one side of biochemistry and drug sensitive plate, add different medicines in the colour developing hole and cross (different medicines, or the soup of same medicine variable concentrations) after, another side is front cover layer plastic sheeting again, makes the aseptic biochemical soup assay plate of sealing.When making drug sensitive detection, lead channel is connected with vacuum pump through valve, utilizes vacuum pump with after inhaling into negative pressure in the quick bacteria identification and susceptibility detecting device, throws open valve, and the bacterium liquid that external atmosphere pressure will prepare in advance, nutrient solution and colored indicator suck in the colour developing hole.After leaving standstill a period of time (for example two hours) under mistake kind of the oxygen barrier state, the degree of observing indicator discoloration can determine zmount of oxygen consumption quickly, thereby learn antibiotic fungistatic effect in each hole.When doing the anaerobion evaluation, put into different biochemical reaction substrates in each hole, utilize anaerobic or hypoxia in the hole, make the anaerobic bacteria growth.According to the difference of each colour developing hole biochemical reaction, can make Rapid identification of anaerobic bacteria and the drug sensitive test of anaerobion.Operation can be made little wedge shape with the insertion end of biochemistry and drug sensitive plate for convenience, or " chamfered edge " is made at its edge handled; Perhaps, with the inside chamfered edge of airtight cover opening or make little hydraucone (insertion end to biochemistry and drug sensitive plate does not deal with); Also can both handle simultaneously.Respectively develop the color among the design quantity and the position in hole can be consistent with the colorimetric hole of microplate reader.Making the aseptic biochemical soup assay plate of sealing, and adding oxysensible color indicator (for example U.S. blue indicator) afterwards, can utilize microplate reader to carry out colorimetric, observing the degree of indicator discoloration through lead channel.For example on 96 hole microplate reader, can once measure one group of data in 96 holes.

The utility model has utilized the principle of advanced " suppressing degree by the partial pressure of oxygen reacting bacteria ", is the instrument that utilizes colourimetry to measure, and observes color relation and can learn the result, operates very easy, quick.Because the impermeability that colour developing hole and oxygen are isolated helps the growth of anaerobion, can carry out the purpose that all kinds of biochemical reactions reach Bacteria Identification easily.The design is a kind of simple in structure, the MIC tools for measurement that is easy to use.The cost of this design own is low, and use cost is also very low, can be suitable for popularization effectively to solve the needs that MIC measures in medical research and the clinical position.

Now be described further with embodiment in conjunction with the accompanying drawings.

Fig. 1 is embodiment 1 mesophytization and drug sensitive plate structural representation;

Fig. 2 is an airtight nested structure synoptic diagram among the same embodiment;

Fig. 3 is a vacuum injector structure synoptic diagram.

Embodiment 1, and with reference to Fig. 1: biochemistry and drug sensitive plate 1 are made by non-toxic plastic, have some colour developings hole 2 on it, and fluid injection groove 3 communicates with each colour developing hole respectively.In the above and below of plate a transparent plastic sheeting 4 is arranged respectively, the size of film is consistent with the plate face, and in the insertion end of plate, plastic sheeting and plate face are pasted together.Edges of boards are made chamfered edge and are handled, and present the very little circular arc of radius.With reference to Fig. 2: airtight cover 5 is also made by non-toxic transparent plastic, be one one end opening apart from the shape box, its inner length and width, consistent with the length and width of biochemistry and drug sensitive plate, its thickness is plate and plastic film thickness sum.The mouth inside edge of airtight cover slightly is domatic, forms a little hydraucone, so that the insertion of biochemistry and drug sensitive plate.During use, between two layers of plastic thin films, making aseptic biochemistry or drug sensitive plate earlier.With the joining prioritization scheme of vacuum pump be, a vacuum injector is set, with reference to Fig. 3: the vacuum injector, by vacuum pump 6, gas admittance valve 7, vent valve 8, filter impact damper 9 and operating room 10 forms.11 is electric controller.Aseptic biochemical drug sensitive plate is put into the operating room, be pumped into negative pressure, the bacterial classification of desire test is made bacterium liquid (including developer, as the blue indicator of U.S.) receive inflow point, open valve, bacterium liquid is drawn in the hole of respectively developing the color by negative pressure.Aseptic drug sensitive plate and plastic sheeting are injected airtight cover, through after a while after (for example two hours), observe the degree of indicator discoloration, present blueness and be bacterium and be killed or grow and suppressed; Indicator fades and is little indigo plant or colourless, shows the bacterial growth breeding, and oxygen is consumed in the nutrient solution; The indicator color obviously is deeper than the minimum antibiotic concentration hole of control wells (hole that does not add soup), is this antibiotic MIC (μ g/ml).Anaerobic bacteria is identified and the susceptibility evaluation is then observed each biochemical hole to sugared tunning, and the indicator discoloration method is observed.

Claims

1, quick bacteria identification and susceptibility detecting device, it is characterized in that: constitute with the airtight cover that is enclosed within its outside by biochemistry and drug sensitive plate, biochemistry and drug sensitive plate are platelets, offer several colour developing holes on it, there is lead channel to communicate with each colour developing hole respectively, airtight cover is the transparent flat box of one one end opening, and its inside dimension and biochemistry and drug sensitive plate are made transition fit closely.

2, according to the described quick bacteria identification and susceptibility of claim 1 detecting device, it is characterized in that: on the surface of biochemistry and drug sensitive plate, add transparent plastic film.

3, according to claim 1 or 2 described quick bacteria identification and susceptibility detecting devices, it is characterized in that: the insertion end of biochemistry and drug sensitive plate is made little wedge shape, or " chamfered edge " made at its edge handled, perhaps, with the inside chamfered edge of airtight cover opening or make little hydraucone.

4, according to claim 1 or 2 described quick bacteria identification and susceptibility detecting devices, it is characterized in that; The quantity and the position in the colour developing hole of biochemistry and drug sensitive plate, consistent with microplate reader colorimetric hole.