KR102197006B1 - Disposible Container for Bacteria - Google Patents

Disposible Container for Bacteria Download PDF

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KR102197006B1
KR102197006B1 KR1020180143247A KR20180143247A KR102197006B1 KR 102197006 B1 KR102197006 B1 KR 102197006B1 KR 1020180143247 A KR1020180143247 A KR 1020180143247A KR 20180143247 A KR20180143247 A KR 20180143247A KR 102197006 B1 KR102197006 B1 KR 102197006B1
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bacteria
culture
sensor unit
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container
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KR20200058745A (en
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신지현
김승철
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주식회사 케이에스
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
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    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • G01—MEASURING; TESTING
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    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

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Abstract

본 발명은 세균의 배양에 따른 그 검출에 있어서 세균의 증식량의 신속한 판별을 목적으로 하는 것으로, 즉, 마개를 갖는 투명한 배양몸체 내부에 세균의 증식유무를 판단하는 광학센서부를 구비하고, 상기 광학센서부는 세균이 소비한 산소의 잔량에 따라 형광의 세기가 변하는 형광센서로 구비한 일회용 세균 콘테이너를 통하여, 투명한 배양몸체 내부에 광학센서부가 발산하는 형광의 세기를 측정하여 세균의 증식량을 측정하며, 이러한 비접촉식의 측정으로 액체배양용 검사기기를 사용함에 발생하였던 검사자의 안전 및 별도의 배양 확인을 위한 공간의 제약의 문제를 해결하고, 세균의 배양 현황 및 배양 양성 판정이 실시간으로 신속한 판독이 이루어지는 효과가 있는 것이다.The present invention is for the purpose of rapid determination of the growth amount of bacteria in the detection according to the cultivation of bacteria, that is, having an optical sensor unit for determining the presence or absence of bacterial growth inside a transparent culture body having a stopper, the optical The sensor unit measures the growth of bacteria by measuring the intensity of fluorescence emitted by the optical sensor unit inside the transparent culture body through a disposable bacteria container equipped with a fluorescence sensor that changes the intensity of fluorescence according to the remaining amount of oxygen consumed by the bacteria. , This non-contact measurement solves the problem of space constraints for the safety of the tester and separate culture confirmation that occurred when using the liquid culture test device, and the status of bacterial culture and the positive culture determination are quickly read in real time. It works.

Description

일회용 세균 콘테이너{Disposible Container for Bacteria}Disposible Container for Bacteria

본 발명은 일회용 세균 콘테이너에 관한 것으로서, 더욱 상세하게는 세균을 배양하는 콘테이너에 있어서 마개를 갖는 투명한 배양몸체 내부에 세균의 증식유무를 판단하는 광학센서부를 구비하고, 상기 광학센서부는 세균이 소비한 산소의 잔량에 따라 형광의 세기가 변하는 형광센서로 구비하여서, 세균의 증식량을 비접촉방식으로 신속하게 판별하는 것을 목적으로 하는 일회용 세균 콘테이너에 관한 것이다.The present invention relates to a disposable bacterial container, and more particularly, in a container for cultivating bacteria, comprising an optical sensor unit for determining the presence or absence of bacteria in a transparent culture body having a stopper, and the optical sensor unit The present invention relates to a disposable bacterial container for the purpose of rapidly discriminating the growth amount of bacteria in a non-contact manner, provided with a fluorescence sensor that changes the intensity of fluorescence according to the remaining amount of oxygen.

일반적으로, 세균의 배양증식은 미생물학, 유전공학, 의학 등에 기본이 되는 작업이다. 이러한 세균의 배양은 콘테이너에 배양액 또는 배지를 무균적으로 주입하고 거기에 배양하고자 하는 세균을 접종한 다음 외부의 오염원이 유입을 차단하는 마개를 무균적으로 밀폐시켜 배양하게 된다. 이러한 콘테이너는 접시형, 튜브형, 병형 등 다양한 형태가 있다.In general, the growth of bacteria is a basic task in microbiology, genetic engineering, and medicine. In the cultivation of these bacteria, a culture medium or a medium is aseptically injected into a container, the bacteria to be cultured are inoculated therein, and then a stopper that blocks the inflow of external contaminants is sealed and cultured aseptically. These containers come in a variety of shapes, such as a dish type, a tube type, and a bottle type.

이러한 세균의 배양증식의 결과를 확인하기 위해서는 일반적으로 검사실의In order to confirm the results of the growth of these bacteria, generally

배양기기 등을 활용하여 확인하였다. It was confirmed using a culture device, etc.

확인하는 방식은 접촉방식과 비접촉 방식으로 구분되는데, 접촉방식은 검사자가 배양된 세균 콘테이너 등을 직접 확인함에 있어 배양액을 채취하여 현미경 검사 또는 PCR 등을 통한 분자생물학적 방법을 통해 확인하는 방식이고, 비접촉방식은 세균이 콘테이너 내부에서 배양되었을 때에 콘테이너의 센싱 등을 통해 콘테이너 외부에서 확인되는 방식이다. The verification method is divided into a contact method and a non-contact method.The contact method is a method in which the inspector directly checks the cultured bacterial container, etc., collecting the culture medium and confirming it through a molecular biological method such as microscopic examination or PCR. The method is a method that is confirmed from the outside of the container through sensing of the container, etc., when bacteria are cultured inside the container.

비접촉방식으로서 세균의 배양상태를 확인하는 방식으로 용존산소의 검출 방식을 이용하는데 그 대표적인 형광물질인 루테늄(II) 복합체(Rudpp)가 사용된다. 이 물질은 용존산소의 농도에 반비례하여 형광을 발생시키는 특성이 있다. Rudpp가 발광하는 형광의 세기를 측정하여 산소의 농도를 측정하는 것이다. 또한, 양자수율과 형광 지속시간이 길어서 광학식 산소센서에 널리 이용되고 있다. Rudpp는 480 nm의 빛에 여기 되면 600 nm의 광을 방출한다.As a non-contact method, a method of detecting dissolved oxygen is used as a method of confirming the culture state of bacteria, and a typical fluorescent substance, Ruthenium(II) complex (Rudpp) is used. This material has the property of generating fluorescence in inverse proportion to the concentration of dissolved oxygen. The concentration of oxygen is measured by measuring the intensity of fluorescence emitted by Rudpp. In addition, the quantum yield and the fluorescence duration are long, so they are widely used in optical oxygen sensors. Rudpp emits 600 nm of light when excited by 480 nm of light.

대한민국 실용신안출원 제 20-2014-0002204호Republic of Korea Utility Model Application No. 20-2014-0002204

2018 기초의학학술대회 “새로운 배양촉진을 위한 액체배양시스템에서의 결핵균 신속배양”(연세대의대 김승철, 김아름, 전보영, 조상래)2018 Basic Medical Conference “Rapid culture of tuberculosis bacteria in a liquid culture system for promotion of new culture” (Yonsei University College of Medicine Kim Seung-cheol, Kim A-reum, Jeon Bo-young, Cho Sang-rae) 초록: Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis). Various culture media have been used for the culture of M. tuberculosis. Bacteriologic culture still remains as an obstruction in the diagnosis of tuberculosis. Growth-Enhancing Media were included a series of concentration of culture-promoting ingredient, 0.0, 0.2, 0.4, 1.0, and 2.0 mg in 7H9 broth. Growth of M. tuberculosis was analyzed by counting CFU using 7H10 agar plates. The effect on the growth of M. tuberculosis was measured in a Liquid Mycobacterial Culture System, MGIT. The Time to Position Detection (TTD) in the media containing growth-enhancing ingredient was reduced by 13.6%, 21.4%, 22.7%, 32.6%.Abstract: Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis). Various culture media have been used for the culture of M. tuberculosis. Bacteriologic culture still remains as an obstruction in the diagnosis of tuberculosis. Growth-Enhancing Media were included a series of concentration of culture-promoting ingredient, 0.0, 0.2, 0.4, 1.0, and 2.0 mg in 7H9 broth. Growth of M. tuberculosis was analyzed by counting CFU using 7H10 agar plates. The effect on the growth of M. tuberculosis was measured in a Liquid Mycobacterial Culture System, MGIT. The Time to Position Detection (TTD) in the media containing growth-enhancing ingredient was reduced by 13.6%, 21.4%, 22.7%, 32.6%.

이에 본 발명은 기존의 세균의 배양과 이를 관측하는 방법에 있어서 침투형 방식의 사용으로 인한 검사자의 안전에 대한 다양한 위험성을 해결하면서 신속하고 편리하면서 정확한 측정이 이루어지고자 하는 것이다.Accordingly, the present invention aims to achieve rapid, convenient and accurate measurement while solving various risks to the safety of an inspector due to the use of an invasive method in the conventional cultivation of bacteria and a method of observing the same.

상술한 목적을 달성하기 위한 본 발명은 콘테이너에 있어서 마개를 갖는 투명한 배양몸체 내부에 세균의 증식유무를 판단하는 광학센서부를 구비하고, 상기 광학센서부는 세균이 소비한 산소의 잔량에 따라 형광의 세기가 변하는 형광센서로 구비한 일회용 세균 콘테이너를 제공하는 것이다. The present invention for achieving the above object is provided with an optical sensor unit for determining the presence or absence of the growth of bacteria inside a transparent culture body having a stopper in the container, the optical sensor unit intensity of fluorescence according to the remaining amount of oxygen consumed by the bacteria. It is to provide a disposable bacterial container equipped with a variable fluorescence sensor.

따라서 투명한 배양몸체 내부에 광학센서부가 발산하는 형광의 세기(세균Therefore, the intensity of fluorescence emitted by the optical sensor unit inside the transparent culture body (bacterial

이 소비한 산소의 잔량에 따라 변함)를 측정하여 세균의 증식량을 측정하는 것이It varies depending on the amount of oxygen consumed) to measure the growth of bacteria.

다. 이러한 비접촉식의 측정으로 액체배양용 검사기기를 사용함에 발생하였던 검사자의 안전 및 별도의 배양 확인을 위한 공간의 제약의 문제를 해결하고, 세균의 배양 현황 및 배양 양성 판정이 실시간으로 신속한 판독이 이루어지는 것이다.All. This non-contact measurement solves the problem of the safety of the tester that occurred when using the liquid culture test device and the limitation of space for a separate culture confirmation, and quickly reads the status of bacterial culture and culture positive determination in real time. .

도 1은 본 발명의 일 실시예를 보인 예시단면도.
도 2는 본 발명에 따라 제작한 실시제작이미지.
도 3은 본 발명에 따른 판센서를 구비한 예를 보인 예시도.
도 4는 본 발명에 따른 광측정센서장치를 구비한 예를 보인 예시도.
도 5는 본 발명에 따른 마개부에 마개센서부를 구비한 예를 보안 예시도.1 is an exemplary cross-sectional view showing an embodiment of the present invention.
Figure 2 is an exemplary production image produced according to the present invention.
Figure 3 is an exemplary view showing an example provided with a plate sensor according to the present invention.
Figure 4 is an exemplary view showing an example provided with a photometric sensor device according to the present invention.
Figure 5 is an example of a security example provided with a plug sensor part in the plug according to the present invention.

본 발명은 세균의 배양에 따른 그 검출에 있어서 세균의 증식량의 신속한 판별을 목적으로 한 것으로서, 본 명세서 및 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니 되며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념을 적절하게 정의할 수 있다는 원칙에 입각하여 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다.The present invention is for the purpose of rapid determination of the growth amount of bacteria in the detection of the bacteria according to culture, and the terms or words used in the present specification and claims are not to be interpreted as being limited to a conventional or dictionary meaning. In addition, the inventor should be interpreted as a meaning and concept consistent with the technical idea of the present invention based on the principle that the concept of terms can be appropriately defined in order to describe his or her invention in the best way.

따라서, 본 명세서에 기재된 실시예와 도면에 도시된 구성은 본 발명의 가장 바람직한 일 실시예에 불과할 뿐이고, 본 발명의 기술적 사상을 모두 대변하는 것은 아니므로, 본 출원시점에 있어서 이들을 대체할 수 있는 다양한 균등물과 변형 예들이 있을 수 있음을 이해하여야 한다. Accordingly, the embodiments described in the present specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, and do not represent all the technical ideas of the present invention, so that they can be replaced at the time of application It should be understood that there may be various equivalents and variations.

즉 본 발명은 세균을 배양 및 증식된 세균을 측정하는 일회용 세균 콘테이너에 관한 것이다.That is, the present invention relates to a disposable bacterial container for culturing bacteria and measuring the grown bacteria.

일회용 세균 콘테이너는 세균을 보관하여 배양하는 몸체가 되는 상부에 입구를 갖는 투명한 배양몸체(110), 상기 배양몸체(110) 상부에 입구를 막아 밀폐하는 마개부(120), 상기 배양몸체(110) 내부 하부에 내부 배양되는 세균의 증식유무를 판단하는 광학센서부(130)로 구비하는 것이다.Disposable bacteria container is a transparent culture body 110 having an inlet at the upper portion of the body for storing and culturing bacteria, a stopper 120 sealing the entrance to the upper portion of the culture body 110, and the culture body 110 It is provided with an optical sensor unit 130 that determines whether or not the growth of bacteria to be cultured inside the lower part.

여기서, 상기 광학센서부(130)은 콘테이너 내에 산소농도에 따라 형광의 세기가 변하는 루테늄(II) 복합체를 기반으로 하는 형광염료를 포함한 형광센서로 구비하는 것이다.Here, the optical sensor unit 130 is provided as a fluorescence sensor including a fluorescent dye based on a ruthenium (II) complex in which the intensity of fluorescence changes according to the oxygen concentration in the container.

또한, 상기 광학센서부(130)의 형광세기를 측정하는 별도의 형광다중분석기를 구비하여 실시할 수 있는 것이다.In addition, it can be carried out by providing a separate fluorescence multiplex analyzer for measuring the fluorescence intensity of the optical sensor unit 130.

또한, 상기 광학센서부(130)은 실리카 분산액에 형광염료를 첨가하여 배양몸체(110) 내부 바닥에 넣고 이를 건조 소결시키는 Sol-Gel 법을 이용 제작하여 여기서 루테늄 복합체(ruthenium complexes) 형광염료의 고정화를 위한 졸-겔(sol-gel) 조성물을 상기 배양몸체(110) 하부에 고정화한 단일체로 구비하여 실시할 수 있는 것이다.In addition, the optical sensor unit 130 is manufactured using a Sol-Gel method in which a fluorescent dye is added to a silica dispersion, placed on the inner floor of the culture body 110, and dried and sintered, whereby the ruthenium complexes are immobilized. For the sol-gel (sol-gel) composition is provided as a single body immobilized in the lower portion of the culture body 110 to be carried out.

또한, 상기 광학센서부(130)은 판형의 판센서(131)로 구비하고, 상기 판센서(131)는 판센서(131)의 바닥 테두리에 콘테이너와 접착되는 접착부(132), 중심에 판센서(131)의 형광세기를 관찰하는 접착하지 않는 관찰부(133)로 구비하여 실시할 수 있는 것이다.In addition, the optical sensor unit 130 is provided as a plate sensor 131, the plate sensor 131 is an adhesive portion 132 adhered to the container on the bottom edge of the plate sensor 131, a plate sensor at the center It can be implemented by having a non-adhesive observation unit 133 for observing the fluorescence intensity of 131.

또한, 상기 광학센서부(130) 상위층에 세균의 배양 속도를 향상하는 액체 또는 젤형의 신속배양배지(201)를 구비하여 실시할 수 있는 것이다.In addition, it can be carried out by providing a liquid or gel-type rapid culture medium 201 that improves the culture rate of bacteria on the upper layer of the optical sensor unit 130.

또한, 상기 배양몸체(110) 내부 하부면 또는 외부 바닥면에 상기 광학센서부(130)의 형광세기의 변화를 측정하는 광측정센서장치(140)을 구비하여 실시할 수 있는 것이다. In addition, it can be carried out by providing an optical measurement sensor device 140 for measuring the change in the fluorescence intensity of the optical sensor unit 130 on the inner bottom surface or the outer bottom surface of the culture body 110.

여기서 상기 광측정센서장치(140)는 형광세기에 따라 색 또는 특정 이미지가 떠오르도록 형성한 디스플레이를 하여 구비할 수 있는 것이다.Here, the photometric sensor device 140 may be provided with a display formed so that a color or a specific image emerges according to fluorescence intensity.

또한, 상기 광측정센서장치(140)는 형광세기에 따라 특정주파수에 신호를 발하는 주파수발생기를 구비할 수 있는 것이다.In addition, the optical measurement sensor device 140 may be provided with a frequency generator that emits a signal at a specific frequency according to the fluorescence intensity.

또한, 상기 마개부(120)는 상부 편의 일부 또는 전체를 투명하게 형성하고, 상기 광학센서부(130)을 배양몸체(110) 내부가 아닌 상기 마개부(120)의 내부 평면에 고정하여 구비하여 실시 할 수 있는 것이다.In addition, the stopper 120 is provided by fixing a part or the whole of the upper part to be transparent, and fixing the optical sensor part 130 to the inner plane of the stopper 120 rather than the inside of the culture body 110 It can be done.

또한, 콘테이너는 초기 외부와 차단되며 상기 광측정센서가 반응하지 않도록 외부와 밀폐된 상태로 형성하여 구비하며, 밀폐된 콘테이너 내부는 질소충전 또는 진공으로 구비하여 실시할 수 있는 것이다.In addition, the container is initially blocked from the outside and is provided in a sealed state with the outside so that the photometric sensor does not react, and the inside of the sealed container can be provided with nitrogen filling or vacuum.

이하, 본 발명의 적용실시과정에 대하여 설명하면 다음과 같다.Hereinafter, the application implementation process of the present invention will be described.

상기한 바와 같이 일회용 세균 콘테이너를 투명한 배양몸체(110), 상부 입구를 막아 밀폐하는 마개부(120), 내부 하부에 광학센서부(130)로 구비하고, 상기 광학센서부(130)은 콘테이너내에 산소농도에 따라 형광의 세기가 변하는 루테늄(II) 복합체를 기반으로 하는 형광염료를 포함한 형광센서로 구비하여 실시하게 되면, 콘테이너내에 세균의 증식되면서 산소를 소모하여 광학센서부(130)이 발하는 형광의 세기가 증가 하는 것이다.As described above, a disposable bacterial container is provided as a transparent culture body 110, a stopper 120 that closes and seals the upper entrance, and an optical sensor unit 130 at the bottom of the inside, and the optical sensor unit 130 is in the container. When implemented with a fluorescent sensor including a fluorescent dye based on a ruthenium (II) complex that changes the intensity of fluorescence depending on the oxygen concentration, the fluorescence emitted by the optical sensor unit 130 consumes oxygen as bacteria grow in the container. The strength of the is to increase.

이로 인해, 세균 배양 검출시스템을 콘테이너에 광학기반의 일체형 센서로 구비하게 되어 별도의 검사장비를 사용하지 않게 되어 검사를 위한 시간 및 공간의 제약이 없으며, 직관적이며 실시간 판별로 신속한 판별이 이루어지는 것이다. 더욱이 비침습적 센서로 인하여 세균의 파괴, 외부 오염물질의 유입방지, 세균의 방출예방의 효과를 갖으며, 검사자의 안전에 대한 다양한 위험성을 해결하는 것이다.For this reason, the bacterial culture detection system is provided as an optical-based integrated sensor in the container, so that a separate inspection equipment is not used, so there is no time and space limitation for inspection, and intuitive and real-time identification allows quick identification. Moreover, due to the non-invasive sensor, it has the effect of destroying bacteria, preventing the introduction of external pollutants, and preventing the release of bacteria, and solving various risks to the safety of inspectors.

또한, 본 발명에 있어서 상기 광학센서부(130)을 Sol-Gel 법을 이용 제작한 단일체로 구비하여 실시하게 되면, 일회용 세균 콘테이너의 제작에 있어서 배양몸체(110) 내부에 광학센서부(130) 용액을 붓고 이를 건조 소결하는 방식으로 광학센서부(130)을 삽입고정함에 별도의 접착제나 물리적 삽입과정이 없어져서 제작이 간편하게 되는 것이다.In addition, in the present invention, if the optical sensor unit 130 is provided as a single body manufactured by using the Sol-Gel method, the optical sensor unit 130 inside the culture body 110 in the manufacture of a disposable bacterial container By pouring the solution and drying and sintering it, there is no separate adhesive or physical insertion process in the optical sensor unit 130 to be inserted and fixed, thereby simplifying manufacturing.

또한, 본 발명에 있어서 상기 광학센서부(130)의 형광의 세기를 상기 형광다중분석기를 이용하여 측정한다면 세균 배양의 증가값의 정확도를 높일 수 있는 것이다.In addition, in the present invention, if the intensity of fluorescence of the optical sensor unit 130 is measured using the fluorescence multiplex analyzer, the accuracy of the increase in bacterial culture can be improved.

또한, 본 발명에 있어서 상기 콘테이너 내부면 또는 외부면에 상기 광학센서부(130)의 형광세기의 변화를 측정하는 광측정센서장치(140)을 구비하여 실시하게 되면, 먼저 디스플레이를 통해 광측정센서장치(140)의 모양을 통해 직관적인 내부 세균의 정도를 확인 가능할 것이다.In addition, in the present invention, if a photometric sensor device 140 for measuring the change in fluorescence intensity of the optical sensor unit 130 is provided on an inner surface or an outer surface of the container, the photometric sensor through the display It will be possible to intuitively check the degree of internal bacteria through the shape of the device 140.

나아가, 상기 광측정센서장치(140)가 주파수발생기를 구비하여 형광에 세기에 따라 다른 주파수 신호를 발하면 이를 수신기로 수신하여 정보를 실시간 모니터링하며 원거리에서 세균 배양의 정보를 수집할 수 있는 것이다.Further, when the photometric sensor device 140 is equipped with a frequency generator and emits a different frequency signal according to the intensity of fluorescence, it is received by a receiver to monitor information in real time and collect information on bacterial culture from a distance.

또한, 본 발명에 있어서 상기 광학센서부(130)을 배양몸체(110) 내부가 아닌 상기 마개부(120) 내부에 구비하여 설치하게 되면 콘테이너에 세균을 주입할 때 광학센서부(130)과 세균의 접촉이 이루어지지 않아서 접촉에 의해 발생하는 문제점을 예방하며, 콘테이너 내부 대기와의 접촉만 이루어져서 내부 산소농도에 대한 측정이 더욱 정확하게 이루어지는 것이다.In addition, in the present invention, if the optical sensor unit 130 is provided and installed inside the closure unit 120 rather than inside the culture body 110, the optical sensor unit 130 and the bacteria are It prevents the problem caused by contact due to the lack of contact with the container, and only the contact with the atmosphere inside the container is made, so that the measurement of the internal oxygen concentration is made more accurately.

또한, 본 발명에 있어서 초기 밀폐된 콘테이너 내부에 질소충전 또는 진공으로 구비하여 실시하게 되면 광학센서부(130)의 반응으로 인한 콘테이너의 훼손 및 노화를 예방하는 것이다.In addition, in the present invention, if the container is initially filled with nitrogen or provided with a vacuum inside the container, damage and aging of the container due to the reaction of the optical sensor unit 130 are prevented.

110: 배양몸체
120: 마개부 121: 마개센서부
130: 광학센서부 131: 판센서
132: 접착부 133: 관찰부
140: 광측정센서장치
201: 신속배양배지110: culture body
120: plug portion 121: plug sensor portion
130: optical sensor unit 131: plate sensor
132: adhesive portion 133: observation portion
140: photometric sensor device
201: Rapid culture medium

Claims (4)

세균을 배양 및 증식된 세균을 측정하는 일회용 세균 콘테이너에 있어서;
일회용 세균 콘테이너는 세균을 보관하여 배양하는 몸체가 되는 상부에 입구를 갖는 투명한 배양몸체(110), 상기 배양몸체(110) 상부에 입구를 막아 밀폐하는 마개부(120), 상기 배양몸체(110) 내부 하부에 내부 배양되는 세균의 증식유무를 판단하는 광학센서부(130)로 구비하고;
상기 광학센서부(130)은 콘테이너 내에 산소농도에 따라 형광의 세기가 변하는 루테늄(II) 복합체를 기반으로 하는 형광염료를 포함한 형광센서로 구비하며;
상기 광학센서부(130)의 형광세기를 측정하는 별도의 형광다중분석기를 구비하고;
상기 광학센서부(130)은 실리카 분산액에 형광염료를 첨가하여 배양몸체(110) 내부 바닥에 넣고 이를 건조 소결시키는 Sol-Gel 법을 이용 제작하여 여기서 루테늄 복합체(ruthenium complexes) 형광염료의 고정화를 위한 졸-겔(sol-gel) 조성물을 상기 배양몸체(110) 하부에 고정화한 단일체로 구비하며;
상기 광학센서부(130)은 판형의 판센서(131)로 구비하고, 상기 판센서(131)는 판센서(131)의 바닥 테두리에 콘테이너와 접착되는 접착부(132), 중심에 판센서(131)의 형광세기를 관찰하는 접착하지 않는 관찰부(133)로 구비하며;
상기 광학센서부(130) 상위층에 세균의 배양 속도를 향상하는 액체 또는 젤형의 신속배양배지(201)를 구비하고;
상기 배양몸체(110) 내부 하부면 또는 외부 바닥면에 상기 광학센서부(130)의 형광세기의 변화를 측정하는 광측정센서장치(140)을 구비하며;
상기 광측정센서장치(140)는 형광세기에 따라 색 또는 특정 이미지가 떠오르도록 형성한 디스플레이를 하고;
상기 광측정센서장치(140)는 형광세기에 따라 특정주파수에 신호를 발하는 주파수발생기를 구비며;
상기 마개부(120)는 상부 편의 일부 또는 전체를 투명하게 형성하고, 상기 광학센서부(130)을 배양몸체(110) 내부가 아닌 상기 마개부(120)의 내부 평면에 고정하여 구비하고;
콘테이너는 초기 외부와 차단되며 상기 광측정센서가 반응하지 않도록 외부와 밀폐된 상태로 형성하여 구비하며, 밀폐된 콘테이너 내부는 질소충전한 것을 특징으로 하는 일회용 세균 콘테이너.
In the disposable bacteria container for measuring the bacteria cultured and grown bacteria;
Disposable bacteria container is a transparent culture body 110 having an inlet at the upper portion of the body for storing and culturing bacteria, a stopper 120 sealing the entrance to the upper portion of the culture body 110, and the culture body 110 It is provided with an optical sensor unit 130 that determines whether or not the growth of bacteria to be internally cultured in the lower inside;
The optical sensor unit 130 is provided as a fluorescence sensor including a fluorescent dye based on a ruthenium (II) complex whose fluorescence intensity varies according to the oxygen concentration in the container;
A separate fluorescence multiplex analyzer for measuring the fluorescence intensity of the optical sensor unit 130;
The optical sensor unit 130 is manufactured by using a Sol-Gel method in which a fluorescent dye is added to the silica dispersion, placed on the inner floor of the culture body 110, and dried and sintered, whereby the ruthenium complexes for immobilizing the fluorescent dye A sol-gel composition is provided as a single body immobilized under the culture body 110;
The optical sensor unit 130 is provided as a plate sensor 131, the plate sensor 131 is an adhesive portion 132 adhered to the container on the bottom edge of the plate sensor 131, a plate sensor 131 at the center. And a non-adhesive observation unit 133 for observing the fluorescence intensity of );
A liquid or gel-type rapid culture medium 201 is provided on the upper layer of the optical sensor unit 130 to increase the culture rate of bacteria;
A photometric sensor device 140 for measuring a change in fluorescence intensity of the optical sensor unit 130 on an inner lower surface or an outer bottom surface of the culture body 110;
The photometric sensor device 140 is configured to display a color or a specific image according to fluorescence intensity;
The photometric sensor device 140 includes a frequency generator that emits a signal at a specific frequency according to the fluorescence intensity;
The stopper 120 is provided to form a part or the whole of the upper part to be transparent, and the optical sensor unit 130 is fixed to the inner plane of the stopper 120, not the inside of the culture body 110;
The container is initially blocked from the outside and provided in a sealed state with the outside so that the photometric sensor does not react, and the inside of the sealed container is filled with nitrogen.
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JP2003521918A (en) * 2000-02-03 2003-07-22 ベクトン・ディキンソン・アンド・カンパニー Transparent sample container

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