CN106187518A - A kind of novel microbiological compound fertilizer material - Google Patents
A kind of novel microbiological compound fertilizer material Download PDFInfo
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- CN106187518A CN106187518A CN201610574788.6A CN201610574788A CN106187518A CN 106187518 A CN106187518 A CN 106187518A CN 201610574788 A CN201610574788 A CN 201610574788A CN 106187518 A CN106187518 A CN 106187518A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The present invention relates to a kind of novel microbiological compound fertilizer material, first the bacterial strain of separation screening is prepared as microbial bacterial agent by certain technique, then the complex fertilizer of a certain amount of microbial inoculum Yu certain formula is prepared as the novel complex fertilizer containing microorganism by physics roll-in prilling.Its viable count of novel complex fertilizer prepared by the present invention is 0.5 hundred million more than CFU/g, and pH value is 5.5 ~ 6.5, and N, P, K content ratio is 1 ~ 2:1:0.8 ~ 1.5.The high temperature resistant resistance of microorganism salt tolerant of the present invention is extremely strong, and the granulating technique of use is gentle, environmental protection, and whole technique is handling by force, green energy conservation.
Description
Technical field
The invention belongs to agricultural fertilizer field, be specifically related to a kind of microbiological compound fertilizer material.
Background technology
Excessively using of chemical fertilizer causes Soil structure destruction, nature biotechnology multiformity and ecosystem stability fall
Degradation the most serious a series of problem under low, the eutrophication of rivers and lakes, agricultural product quality.Microbial manure, refers to contain
There are the goods of specified microorganisms live body, are applied to agricultural production, by the vital movement of microorganism contained therein, increase plant and support
The supply divided or promotion plant growing, improve yield, improve quality of agricultural product and agroecological environment.Developing modern agriculture,
Realizing agricultural sustainable development, the development to fertilizer industry proposes new higher requirement.And microbial manure is ploughed in lifting
The aspects such as geological measuring, improvement widespread pollution from the overuse of fertilizers and pesticides in rural area, lifting quality of agricultural product have irreplaceable effect, widely popularize application raw
Thing fertilizer is the major action realizing agricultural sustainable development.
The research and development pattern of China's bio-feritlizer the most more tradition, apparatus and process is not enough, does not the most become bio-fertilizer
The Innovation Main Body of material, there is also some clearly disadvantageous: 1) living bacteria count of fertilizer is the lowest；2) strain was compounding with chemical fertilizer
In journey, mortality rate is high, causes addition bacterium amount many, and survival rate is low；3) traditional microbial manure uses a large amount of organic as filling out
Material, causes miscellaneous bacteria amount reproduction during depositing, and miscellaneous bacteria rate is higher；4) decline of process bacterium amount is deposited rapidly by compound fertilizer, guarantees the quality
Phase is short；5) content of beary metal of microbial manure is higher.
Summary of the invention
Present invention aim at providing a kind of novel microbiological compound fertilizer material, for overcoming existing microbial manure not
Foot, and there is higher viable bacteria content, Microbial survival rate is higher, and has more preferable fertilizer efficiency.
For achieving the above object, the present invention is by the following technical solutions:
A kind of microbiological compound fertilizer material, it is characterised in that it is proportionally passed through physics roll-in prilling by following components
It is prepared from: microbial bacteria 0.2-0.5 weight portion, ammonium sulfate 40-50 weight portion, potassium sulfate 20-30 weight portion, ammonium dihydrogen phosphate
25-35 weight portion, carbamide 10-20 part.
Described microbial bacterial agent is to be prepared from according to following steps:
1) product A and fermentation seed liquid are prepared: acid-base modifier and water are added separately in the plant residue after drying,
In fermentation tank, high temperature sterilize 15～30min at 85 DEG C～130 DEG C, obtains product A, standby, the plant residue after drying, soda acid
Mass ratio between regulator and water is 1000: 1～10: 250～500；
To be seeded in fluid medium addicted to sugar bacillus cereus, and cultivate 20～30 hours at 25 DEG C～35 DEG C, obtain
Fermentation seed liquid, standby, the mass ratio addicted to sugar bacillus cereus and fluid medium is 2～15: 100；
2) product B is prepared: joined by fermentation seed liquid according to the mass ratio 1～15: 100 of fermentation seed liquid Yu product A
In product A, ferment 3～10 days under the conditions of 25 DEG C～35 DEG C, pH5～8, obtain product B；
3) prepare products C: adjust the pH to 4.5～5 of product B, and temperature in fermentation tank is cooled to 0 DEG C～8 DEG C, preserve
3～5 days, then temperature is recovered to 25 DEG C～35 DEG C, obtain products C；
4) product D is prepared: according to the mass ratio 1～15: 100 of fermentation seed liquid and products C by step 1) seed prepared
Liquid joins in products C, 25 DEG C～35 DEG C, ferment 3～10 days under the conditions of pH 5～8, obtain product D；
5) microbial bacterial agent is prepared: adjust the pH to 4.5～5 of product D, and temperature in fermentation tank is cooled to 0 DEG C～8
DEG C, preserve 3～5 days, then temperature is recovered to 25 DEG C～35 DEG C, eventually pass and be dried to obtain microbial bacterial agent；
Described plant residue is Thallus Laminariae (Thallus Eckloniae), Entermorpha, bean cake, cottonseed meal, Testa Tritici, wheat stalk, corn straw, furfural dregs and mushroom
A kind of or arbitrary proportion in mushroom slag two or more；
Described acid-base modifier be two kinds of a kind of or arbitrary proportion in plant ash, Calx, citric acid and oxalic acid with
On.
Described its viable count of microbiological compound fertilizer material is 0.5 hundred million more than CFU/g, and pH value is 5.5～6.5, N, P, K content
Ratio is 1～2: 1: 0.8～1.5.
Described is that its preserving number is CGMCC No12155 addicted to sugar bacillus cereus YIC～Alg3 addicted to sugar bacillus cereus.
Beneficial effects of the present invention:
1. the living bacteria count of the Chemical Mixed Fertilizer of the present invention is high, all at 0.5 hundred million more than CFU/g.
2. the present invention uses physics roll-in granulating technique to be compounded with complex fertilizer by microbial bacterial agent, whole process green ring
Protect, it is to avoid high temperature and the chemical reagent erosion to microorganism, ensure that the survival rate of microorganism to greatest extent.
3. the strain that the present invention uses has high-salt tolerance, heat-resisting quantity, can directly compound with Chemical Mixed Fertilizer inorganic salt, and
The environment of this high salt makes other miscellaneous bacterias not survive, and it also avoid that a large amount of organic interpolations causes deposits process simultaneously
In the breeding of a large amount of miscellaneous bacterias cause the putrid and deteriorated of fertilizer, greatly reduce miscellaneous bacteria rate, it is ensured that the high-quality of fertilizer.
4. the strain that the present invention uses has high resistance, overcomes tradition microbial inoculum in hypersaline environment long-term storage process
The drawback of middle mortality, greatly reduces the mortality rate of strain, it is ensured that living bacteria count, substantially prolongs guaranteeing the quality of fertilizer
Phase.
5. the material of the use present invention and the microbiological compound fertilizer material content of beary metal of technique production are relatively low.
Accompanying drawing explanation
Fig. 1 is microexamination YIC～the Gram’s staining of Alg3 bacterial strain and assembles agglomerating phenomenon figure.
Fig. 2 is YIC～Alg3 bacterial strain phylogenetic tree schematic diagram based on 16SrDNA sequence construct.
Detailed description of the invention
In conjunction with specific embodiments technical scheme is described in further detail, but not as to the present invention's
Limit.
Embodiment 1: a kind of novel complex fertilizer
Preparation product A and fermentation seed liquid: acid-base modifier and water are added separately in the plant residue after drying,
In fermentation tank, high temperature sterilize 30min at 100 DEG C, obtains product A, standby, plant residue, acid-base modifier and water after drying it
Between mass ratio be 1000: 5: 300；
Described plant residue is 1 part of Thallus Laminariae (Thallus Eckloniae), Entermorpha 1 part, bean cake 2 parts, and acid-base modifier is plant ash 1 part, citric acid 1
Part,
To be seeded in fluid medium addicted to sugar bacillus cereus YIC～Alg3, and cultivate 20 hours at 25 DEG C, be sent out
Ferment seed liquor, standby, the mass ratio addicted to sugar bacillus cereus YIC～Alg3 and fluid medium is 7: 100；
2) product B is prepared: according to the mass ratio 5: 100 of fermentation seed liquid Yu product A, fermentation seed liquid is joined product A
In, ferment 5 days under the conditions of 27 DEG C, pH7～8, obtain product B；
3) products C is prepared: adjust the pH to 4.5～5 of product B, and temperature in fermentation tank is cooled to 2 DEG C, preserve 3 days,
Then temperature is recovered to 27 DEG C, obtain products C；
4) product D is prepared: according to the mass ratio 7: 100 of fermentation seed liquid and products C by step 1) seed liquor prepared adds
Enter in products C, 27 DEG C, ferment 8 days under the conditions of pH 5～6, obtain product D；
5) prepare microbial bacterial agent: adjust the pH to 4.5～5 of product D, and temperature in fermentation tank is cooled to 7 DEG C, preserve
4 days, then temperature is recovered to 30 DEG C, eventually pass and be dried to obtain microbial bacterial agent；
6) microbiological compound fertilizer material is prepared: following components be proportionally prepared from by physics roll-in granulating technique:
Microbial bacterial agent 0.3 weight portion, ammonium sulfate 40 weight portion, potassium sulfate 25 weight portion, ammonium dihydrogen phosphate 25 weight portion, carbamide 17 weight
Amount part.
Embodiment 2: a kind of novel complex fertilizer
Preparation product A and fermentation seed liquid: acid-base modifier and water are added separately in the plant residue after drying,
In fermentation tank, high temperature sterilize 15min at 121 DEG C, obtains product A, standby, plant residue, acid-base modifier and water after drying it
Between mass ratio be 1000: 4: 310；
Described plant residue is 1 part of Testa Tritici, wheat stalk 1 part, cottonseed meal 1 part, bean cake 2 parts, and acid-base modifier is vegetation
Ash 1 part, oxalic acid 1 part,
To be seeded in fluid medium addicted to sugar bacillus cereus YIC～Alg3, and cultivate 24 hours at 30 DEG C, be sent out
Ferment seed liquor, standby, the mass ratio addicted to sugar bacillus cereus YIC～Alg3 and fluid medium is 10: 100；
2) product B is prepared: according to the mass ratio 10: 100 of fermentation seed liquid Yu product A, fermentation seed liquid is joined product
In A, ferment 8 days under the conditions of 30 DEG C, pH6～7, obtain product B；
3) products C is prepared: adjust the pH to 4.5～5 of product B, and temperature in fermentation tank is cooled to 3 DEG C, preserve 4 days,
Then temperature is recovered to 25 DEG C, obtain products C；
4) product D is prepared: according to the mass ratio 10: 100 of fermentation seed liquid and products C by step 1) seed liquor prepared adds
Enter in products C, 30 DEG C, ferment 7 days under the conditions of pH 6～7, obtain product D；
5) prepare microbial bacterial agent: adjust the pH to 4.5～5 of product D, and temperature in fermentation tank is cooled to 3 DEG C, preserve
5 days, then temperature is recovered to 30 DEG C, eventually pass and be dried to obtain microbial bacterial agent；
6) microbiological compound fertilizer material is prepared: following components be proportionally prepared from by physics roll-in granulating technique:
Microbial bacterial agent 0.4 weight portion, ammonium sulfate 45 weight portion, potassium sulfate 20 weight portion, ammonium dihydrogen phosphate 30 weight portion, carbamide 15 weight
Amount part.
Embodiment 3: a kind of novel complex fertilizer
Preparation product A and fermentation seed liquid: acid-base modifier and water are added separately in the plant residue after drying,
In fermentation tank, high temperature sterilize 30min at 121 DEG C, obtains product A, standby, plant residue, acid-base modifier and water after drying it
Between mass ratio be 1000: 6: 420；
Described plant residue is 2 parts of Testa Tritici, wheat stalk 1 part, corn straw 1 part, furfural dregs 1 part, acid-base modifier
For Calx 1 part, oxalic acid 1 part,
To be seeded in fluid medium addicted to sugar bacillus cereus YIC～Alg3, and cultivate 30 hours at 27 DEG C, be sent out
Ferment seed liquor, standby, the mass ratio addicted to sugar bacillus cereus YIC～Alg3 and fluid medium is 13: 100；
2) product B is prepared: according to the mass ratio 13: 100 of fermentation seed liquid Yu product A, fermentation seed liquid is joined product
In A, ferment 4 days under the conditions of 33 DEG C, pH6～7, obtain product B；
3) products C is prepared: adjust the pH to 4.5～5 of product B, and temperature in fermentation tank is cooled to 6 DEG C, preserve 5 days,
Then temperature is recovered to 30 DEG C, obtain products C；
4) product D is prepared: according to the mass ratio 13: 100 of fermentation seed liquid and products C by step 1) seed liquor prepared adds
Enter in products C, 33 DEG C, ferment 4 days under the conditions of pH 6～7, obtain product D；
5) prepare microbial bacterial agent: adjust the pH to 4.5～5 of product D, and temperature in fermentation tank is cooled to 2 DEG C, preserve
5 days, then temperature is recovered to 25 DEG C, eventually pass and be dried to obtain microbial bacterial agent；
6) microbiological compound fertilizer material is prepared: following components be proportionally prepared from by physics roll-in granulating technique:
Microbial bacterial agent 0.5 weight portion, ammonium sulfate 40 weight portion, potassium sulfate 30 weight portion, ammonium dihydrogen phosphate 25 weight portion, carbamide 12 weight
Amount part.
Embodiment 4: a kind of novel complex fertilizer
Preparation product A and fermentation seed liquid: acid-base modifier and water are added separately in the plant residue after drying,
In fermentation tank, high temperature sterilize 30min at 125 DEG C, obtains product A, standby, plant residue, acid-base modifier and water after drying it
Between mass ratio be 1000: 7: 500；
Described plant residue is mushroom residue 2 parts, wheat stalk 1 part, corn straw 1 part, furfural dregs 1 part, acid-alkali accommodation
Agent is Calx 2 parts, citric acid 1 part,
To be seeded in fluid medium addicted to sugar bacillus cereus YIC～Alg3, and cultivate 22 hours at 35 DEG C, be sent out
Ferment seed liquor, standby, the mass ratio addicted to sugar bacillus cereus YIC～Alg3 and fluid medium is 6: 100；
2) product B is prepared: according to the mass ratio 6: 100 of fermentation seed liquid Yu product A, fermentation seed liquid is joined product A
In, ferment 3 days under the conditions of 35 DEG C, pH5～6, obtain product B；
3) products C is prepared: adjust the pH to 4.5～5 of product B, and temperature in fermentation tank is cooled to 4 DEG C, preserve 3 days,
Then temperature is recovered to 27 DEG C, obtain products C；
4) product D is prepared: according to the mass ratio 7: 100 of fermentation seed liquid and products C by step 1) seed liquor prepared adds
Enter in products C, 35 DEG C, ferment 3 days under the conditions of pH 6～7, obtain product D；
5) prepare microbial bacterial agent: adjust the pH to 4.5～5 of product D, and temperature in fermentation tank is cooled to 3 DEG C, preserve
3 days, then temperature is recovered to 28 DEG C, eventually pass and be dried to obtain microbial bacterial agent；
6) microbiological compound fertilizer material is prepared: following components be proportionally prepared from by physics roll-in granulating technique:
Microbial bacterial agent 0.3 weight portion, ammonium sulfate 40 weight portion, potassium sulfate 26 weight portion, ammonium dihydrogen phosphate 35 weight portion, carbamide 10 weight
Amount part.
Comparative example 1: a kind of complex fertilizer containing microorganism
Prepare microbiological compound fertilizer material: following components be proportionally prepared from by physics roll-in granulating technique: city
Sell bacillus subtilis microbial agent 0.5 weight portion, ammonium sulfate 40 weight portion, potassium sulfate 30 weight portion, ammonium dihydrogen phosphate 25 weight portion,
Carbamide 12 weight portion.
Comparative example 2: a kind of complex fertilizer containing microorganism
Prepare microbiological compound fertilizer material: following components be proportionally prepared from by physics roll-in granulating technique: city
Sell bacillus licheniformis agent 0.3 weight portion, ammonium sulfate 40 weight portion, potassium sulfate 26 weight portion, ammonium dihydrogen phosphate 35 weight portion,
Carbamide 10 weight portion.
Comparative example 3: a kind of complex fertilizer containing microorganism
Prepare microbiological compound fertilizer material: following components be proportionally prepared from by physics roll-in granulating technique: city
Sell bacillus megaterium microbial inoculum 0.3 weight portion, ammonium sulfate 40 weight portion, potassium sulfate 25 weight portion, ammonium dihydrogen phosphate 25 weight portion,
Carbamide 17 weight portion.
Product checking: product indices is entered according to People's Republic of China (PRC) agricultural industry criteria NY/T 798-2004
Row detection, testing result is shown in Table 1, table 2.
Table 1: the embodiment of the present invention and the quality measurements of comparative example
Table 2: the living bacteria count testing result (unit: hundred million cfu/g) of the embodiment of the present invention and comparative example
| Project | March | June | JIUYUE | December | 18 months | 24 months |
| Embodiment 1 | 0.83 | 0.83 | 0.79 | 0.73 | 0.64 | 0.58 |
| Embodiment 2 | 0.87 | 0.85 | 0.78 | 0.70 | 0.63 | 0.54 |
| Embodiment 3 | 0.92 | 0.90 | 0.85 | 0.77 | 0.66 | 0.57 |
| Embodiment 4 | 0.85 | 0.84 | 0.77 | 0.71 | 0.63 | 0.55 |
| Comparative example 1 | 0.88 | 0.72 | 0.61 | 0.53 | 0.22 | 0.11 |
| Comparative example 2 | 0.91 | 0.77 | 0.58 | 0.44 | 0.17 | 0.05 |
| Comparative example 3 | 0.87 | 0.71 | 0.49 | 0.38 | 0.09 | 0.02 |
Result above shows, the microbial manure indices that the present invention produces is obviously higher than national standard, due to this
It is resistance that the bacterial strain that invention uses has high-salt tolerance, and miscellaneous bacteria rate substantially reduces, and colibacillus of excrement number is almost nil, and ascarid is dead
Rate 100%.And through the storage of 24 months, the living bacteria count in embodiments of the invention all can reach 0.5 hundred million/more than g,
And other strain quantity used in comparative example declines rapidly.
Embodiments of the invention and comparative example related fertilizer are imposed on growing tomatoes plot, and mu consumption is 50kg.Plantation
Phase is 1 year, and soil property specific performance index is shown in Table 3.
Table 3: the property indices after soil improvement
Result shows: use the microbial manure of the present invention, soil function be improved significantly, aeration porosity is
13%-16%, soil pH 6.1-6.4, soil weight 1.49-1.57g/cm3, content of beary metal reduces the substantially micro-life of soil
Thing content is improved significantly.
Use embodiment and comparative example product in the present invention and Fructus Lycopersici esculenti crop affected situation, and use what market was bought
Bacterial manure series products contrasts, and fertilizer efficiency the results are shown in Table 4.
Table 4: fertilizer efficiency comparing result
Result shows: in the case of dose is suitable, uses the microbiological compound fertilizer product of the present invention, hence it is evident that promotes and makees
Article matter, greatly reduces effect pest and disease damage, effectively the resistance of enhancing crop, energy raising effect yield 9-12%,
Preserving number addicted to sugar bacillus cereus YIC～Alg3 of the present invention is CGMCC No.12155, depositary institution's title:
China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution address: BeiChen West Road, Chaoyang District, BeiJing City 1
Number institute 3, preservation date: on 03 02nd, 2016, Latin Classification And Nomenclature: Bacillus halosaccharovorans.
The described biological nature addicted to sugar bacillus cereus YIC～Alg3 is as follows:
Alginic acid carbon source culture medium: sodium alginate 5g, ammonium sulfate 5g, magnesium sulfate 1g, dipotassium hydrogen phosphate 2g, ferrous sulfate
0.01g, agar 20g, distilled water 1000ml, pH7.2～7.4.
After YIC～Alg3 bacterial strain grows 48h on alginic acid sole carbon source flat board, bacterium colony is irregular, and faint yellow, centre is not
Transparent, edge is translucent, surface folding.It is shaft-like that microscope observes bacterial strain, can produce spore, gram at end or proximal end
The positive, can move, and Later growth thalline easily assembles agglomerating (Fig. 1).Growth temperature range 20～45 DEG C, PH scope 6～9, NaCl
Concentration range 0～15%, bacterial strain optimum growth temperature 37 DEG C, the most suitable growth pH 7.5.Catalase, oxidase, VP tests the positive, energy
Enough utilize glucose, mannose, mannitol, fructose, lactose, galactose, starch；Sorbitol, cellulose can not be utilized.System is sent out
Educate tree as shown in Figure 2.According to its physiological and biochemical property, 16SrDNA sequence and phylogenetic tree information, by this identification of strains be addicted to
Sugar bacillus halosaccharovorans.
Note :+represent and can utilize ,～represent and can not utilize.
Claims (4)
1. a microbiological compound fertilizer material, it is characterised in that it is proportionally passed through physics roll-in prilling system by following components
For forming: microbial bacterial agent 0.2-0.5 weight portion, ammonium sulfate 40-50 weight portion, potassium sulfate 20-30 weight portion, ammonium dihydrogen phosphate
25-35 weight portion, carbamide 10-20 part.
2. microbiological compound fertilizer material as claimed in claim 1, it is characterised in that: described microbial bacterial agent is according to following steps
It is prepared from:
1) prepare product A and fermentation seed liquid: acid-base modifier and water are added separately in the plant residue after drying, sending out
In ferment tank, high temperature sterilize 15～30min at 85 DEG C～130 DEG C, obtains product A, standby, the plant residue after drying, acid-alkali accommodation
Mass ratio between agent and water is 1000:1～10:250～500；
To be seeded in fluid medium addicted to sugar bacillus cereus, and cultivate 20～30 hours at 25 DEG C～35 DEG C, be fermented
Seed liquor, standby, the mass ratio addicted to sugar bacillus cereus and fluid medium is 2～15:100;
2) product B is prepared: according to the mass ratio 1～15:100 of fermentation seed liquid Yu product A, fermentation seed liquid is joined product A
In, ferment 3～10 days under the conditions of 25 DEG C～35 DEG C, pH5～8, obtain product B；
3) prepare products C: adjust the pH to 4.5～5 of product B, and temperature in fermentation tank is cooled to 0 DEG C～8 DEG C, preserve 3～5
My god, then temperature is recovered to 25 DEG C～35 DEG C, obtain products C；
4) product D is prepared: seed liquor step 1) prepared according to the mass ratio 1～15:100 of fermentation seed liquid with products C adds
Enter in products C, 25 DEG C～35 DEG C, ferment 3～10 days under the conditions of pH 5～8, obtain product D；
5) prepare microbial bacterial agent: adjust the pH to 4.5～5 of product D, and temperature in fermentation tank is cooled to 0 DEG C～8 DEG C, protect
Deposit 3～5 days, then temperature is recovered to 25 DEG C～35 DEG C, eventually pass and be dried to obtain microbial bacterial agent；
Described plant residue is Thallus Laminariae (Thallus Eckloniae), Entermorpha, bean cake, cottonseed meal, Testa Tritici, wheat stalk, corn straw, furfural dregs and mushroom residue
In a kind of or arbitrary proportion two or more；
Described acid-base modifier is the two or more of a kind of or arbitrary proportion in plant ash, Calx, citric acid and oxalic acid.
3. microbiological compound fertilizer material as claimed in claim 1, it is characterised in that: described its viable count of microbiological compound fertilizer material
Being 0.5 hundred million more than CFU/g, pH value is 5.5 ~ 6.5, and N, P, K content ratio is 1 ~ 2:1:0.8 ~ 1.5.
4. the microbiological compound fertilizer material as described in claim 1 or 2 or 3, it is characterised in that: described is addicted to sugar addicted to sugar bacillus cereus
Bacillus cereus YIC～Alg3, its preserving number is CGMCC No12155.
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