CN106177768A - Chinese prescription of one group for the treatment of hyperthyroidism and preparation method thereof - Google Patents

Abstract

The invention discloses Chinese prescription of one group for the treatment of hyperthyroidism and preparation method thereof, it is made up of following component and weight portion thereof: fresh Cymbidium ensifolium (L.) Sw. root stem 30 35, fresh Folium Stevlae Rebaudianae 50 60, Spica Prunellae 10 12, Galla Chinensis 10 12, Radix Adenophorae (Radix Glehniae) 13 15, Sargassum 12 15, field winter 13 15, Radix Ophiopogonis 13 15, Fructus Citri tangerinae core 13 15, the Radix Rehmanniae 13 15, pollen 10 12, Thallus Laminariae (Thallus Eckloniae) 10 12, Gekko Swinhonis 10 12, Rhizoma Curcumae 10 12, Herba Scutellariae Barbatae 10 12, Fructus Polygoni Orientalis 10 12, Bufo siccus 10 12, fresh Rhizoma dioscoreae 50 60.The Chinese prescription of one group for the treatment of hyperthyroidism of the present invention, Bufo siccus has effect of heat clearing away, toxin expelling, antitumor, defying age；It is equipped with Herba Scutellariae Barbatae, Spica Prunellae, Radix Ophiopogonis, there is inducing diuresis to remove edema, yin nourishing regulating menstruation, anticancer function.

Description

Chinese prescription of one group for the treatment of hyperthyroidism and preparation method thereof

Technical field

The present invention relates to processing technique of Chinese herbs field, particularly relate to Chinese prescription and the preparation side thereof of one group for the treatment of hyperthyroidism Method.

Background technology

At present Chinese medicine processing Mechanism Study is mainly used chemical and pharmacological method, and bioconversion, intestinal absorption Transhipment, body absorption distribution and these new approaches of metabolism group and new method application are less.Therefore grind from Chinese medicine processing mechanism Studying carefully present situation on the whole, comprehensive further investigation still is apparent not enough, and the elaboration majority of Chinese medicine processing mechanism is rested on this process The two ends i.e. change of chemical composition and pharmacologic agent change, and to illustrate concoct mechanism key link--in before and after the process of preparing Chinese medicine The research such as the Absorption And Metabolism of medicine active component is the most deep enough, before lacking a frame communication Chinese medicine processing in concocting mechanism elaborate process Bridge--the research of active ingredient of Chinese herbs Absorption And Metabolism of relation between rear chemical composition change and pharmacological effect change.For oral use Medicine is the Main Means of Chinese medicine, and it is that Chinese medicine produces biology that active ingredient of Chinese herbs absorbs with metabolism during gastrointestinal transit The important step of effect, the most thoroughly illustrates the optimal point of penetration of Chinese medicine processing mechanism.And Chinese medicine generally requires and overcomes intestinal to inhale The effect receiving barrier network could be absorbed, thus plays its therapeutical effect.Intestinal absorption barrier network is the important protection of body System, can stop the absorption of poisonous substance and antigen, but be also the principal element limiting drug absorption, mainly by chemical barrier, physics Barrier and biochemical barrier composition.Chemical barrier is mainly made up of the mucus of intestinal mucosa epithelial secretion, Digestive system etc., disappears containing various Changing enzyme such as Lactose enzyme Phlorizin hydrolase (LPH), protide, many peptides and a lot of Chinese medicine ingredients can be broken by intestinal chemical barrier Bad.Physical barriers is by structure, fully functional intestinal mucosa epithelium and is covered in the mucus on its surface and constitutes, and slime layer can be effectively Protective epithelium cell microvillus, from mechanical, chemical damage, is also the obstacle of drug absorption simultaneously；And cell membrane and iuntercellular Compact siro spinning technology be then the principal element limiting drug absorption to the permeability of medicine.Biochemical barrier is mainly by enterocyte Metabolic enzyme and multi-efflux pumps are constituted, and such as II phase metabolic enzymes such as cytochrome enzyme and glucuronyl transferases, can will enter Enter metabolism before the relative medicine metabolism of cell, i.e. body circulation；P-glycoprotein (P-gp), multidrug-associated protein (MRP), first ammonia Intracellular relative medicine " pump " can be gone out extracellular by the discharging systems such as petrin (MTX), thus reduces drug concentration.Cause This, carry out intestinal absorption barrier network to active component before and after Chinese medicine processing or the influence research of component Absorption And Metabolism, can be deeper Illustrate Chinese medicine processing mechanism with entering, obtain the most objectively data from whole animal, cell and subcellsular level, for disclosing Chinese medicine Concoct theoretical scientific meaning and new thinking and method are provided.

The most white etc. utilize esi-msn to analyze Radix Aconiti Lateralis Preparata to add the chemical composition change before and after adjuvant Radix Glycyrrhizae is concocted, take off Show Radix Glycyrrhizae the attenuation mechanism in Radix Aconiti Lateralis Preparata concocting process.Research shows composition in Radix Glycyrrhizae diester-type alkaloids easily and in Radix Aconiti Lateralis Preparata And thermal decomposition product generation transesterification reaction, generate the lipo-alkaloids that toxicity is less；There is a small amount of diester-type alkaloids simultaneously With the precipitation that the chemical composition in Radix Glycyrrhizae defines slightly solubility, make the content of diester-type alkaloids in Radix Aconiti Lateralis Preparata reduce, thus reach The purpose of attenuation.Bioconversion method application in Chinese medicine processing Mechanism Study is mainly reflected in 2 aspects.One is to send out In the process of preparing Chinese medicine Mechanism Study of ferment Chinese medicine.Fermentation method is always one of Chinese medicine processing method, and it is by the effect of microorganism, in change The original property of medicine of medicine, improves curative effect, reduces toxic and side effects, expands indication.Chinese medicine is concocted actually hence with fermentable Being a kind of bioconversion reaction, available microbiology and biotechnology scheduling theory illustrate the principle of fermentation.As red in Wang Qiu based on The cooked theory being effective substance research fermentation class Chinese medicine Massa Medicata Fermentata with secondary metabolites from microorganisms and bioconversion product； The impact on Flos Carthami antioxidant activity is concocted in the microorganisms fermentations such as Feng Zhihua, and is inquired into its mechanism, result table Bright Flos Carthami is concocted its anti-oxidation efficacy through the fermentation of Bacillus licheniformis C2-13 and is significantly improved, and its reason is probably C2-13 Produced various glycoside hydrolases, by many by the glucoside compound water of phenolic hydroxyl group with glucose condensation in Flos Carthami Solve, make the number of phenolic hydroxyl group increase substantially, thus improve the non-oxidizability of Flos Carthami；In addition the aglycon produced after hydrolysis ratio is corresponding Glucosides easily absorb, bioavailability is higher, is more beneficial for playing its pharmacological action.Two is to imitate in Chinese medicine processing forebody-afterbody Answer in material base research and apply.Chinese medicine mainly with administered in oral forms, oral Chinese medicine composition play drug action it Before, in addition to producing dynamically change in the process of preparing Chinese medicine, decoction process, after entering body, also want occurrence dynamics to change, this change First process is exactly by human body intestinal canal enzyme and intestinal metabolism in digestive tract.

Chinese medicine processing has stir-fry method, preparation method, forges method, cooking process, duplicating method, baking process, stewing method etc., and these concocting methods are both needed to Heating, heating process can make the chemical composition of Chinese medicine convert, thus change clinical efficacy；The Clinical practice of Chinese medicine is with decoction It is main for being administered orally, and medicine is directly exposed to intestinal microbial population after entering enteric cavity, possibly together with various active enzyme in enteric cavity, and these intestinal microbial populations With the existence of intestinal digestive enzyme, together with the liver of body, the joint effect conversion of chemical composition of Chinese materia medica；Extract oral is inhaled Receipts process occurs mainly in small intestinal, before and after the process of preparing Chinese medicine chemical composition at intestinal absorption barrier network it may happen that interact and affect it Absorption and transport, thus affect the bioavailability of medicine and the curative effect of processed product.Therefore author propose study hypothesis: " inside and outside Chemical composition converts can affect the absorption and transport of chemical composition after the process of preparing Chinese medicine with the regulation and control of intestinal absorption barrier network and couple thereof, Thus change the curative effect of Chinese medicine processing product ".

Sugar, as one of three class biomolecule most important in life entity, but lags behind nucleic acid and albumen to its understanding Matter.Main cause is the multiformity of sugar structure.Sugar is connected by glycosidic bond, and glycosidic bond has two kinds of stereoisomeric forms in any ratio of α, β, due to Each monosaccharide has several hydroxyl often to form branched structure.The synthesis of each glycosidic bond or fracture are by being positioned ad-hoc location Glycosyl transferase or hydrolytic enzyme control, and this process is coarse, causes " microheterogeneity " of sugar chain so that it is separate pole Its difficulty.The multiformity of sugar structure so that the quantity of information that it may carry is considerably beyond protein and nucleic acid.Saccharide compound Being one of most important medicine of the mankind, the sugared drug molecule currently used includes various antibiotic, nucleoside, polysaccharide and glycolipid etc., For almost all of disease.The effective ingredient of China's Chinese medicine is also most relevant with sugar.Sugar in many plant extracts Glycoside compound, is the sugar a kind of principal mode in nature existence, provides extremely abundant medicine resource for the mankind, common Glucosides medicine such as cardiac glycoside, salicin, flavonoid glycoside etc. oneself be applied to clinic.In recent years, the rise of chemistry glycobiology research With scientists to the progressively understanding of sugar function in life process, it is greatly promoted the innovation research of sugar medicine, occurs in that saliva The new sugared medicines such as liquid acid derivative, saccharide vaccines, heparin mimetic.

Herba Lycopi has promoting blood flow to regulate menstruation, blood stasis dispelling eliminating carbuncle, effect of inducing diuresis to remove edema, for blood circulation promoting and blood stasis dispelling apoplexy due to endogenous wind conventional clinically Medicine.Herba Lycopi mainly contains volatile oil, glucoside, tannin, resin, flavonoid glycoside, phenols, saponin, aminoacid, organic acid, water Threose, galactose, fructose etc..Modern study shows that Herba Lycopi can significantly improve the abnormal erythrocyte rheology index of blood stasis animal, right Rat chronic renal failure has an improvement result, simultaneously protecting the liver, the aspect such as hepatoprotective, anti-acute hepatic failure all have preferable activity.Warp Crossing the unremitting effort of modern science, find that in the rhizome of Herba Lycopi, content of stachyose is high, Herba Lycopi's oligosaccharide is mainly by stachyose, hair Stamen spends sugar and Raffinose to form, and wherein the content of stachyose and verbascose is obviously higher than the oligosaccharide in Semen sojae atricolor.Therefore, money The abundant plant Herba Lycopi in source oneself develop the sustainable development natural product raw material becoming presently commercially available stachyose product rapidly.Herba Stachydis Japonicae Sugar as emerging functional oligose, because of its slow down aging, regulation micro ecology of gastrointestinal tract balance, the immunity that improves, toxin expelling, Lowering blood pressure aspect has physiological function, and its development and application is increasingly subject to pay attention to.Raffinose has improvement to atopic dermatitis Effect, it is possible to as the active component of hepatopathy inhibitor, also has anti-hemopathic effect Proteinaceous, anti-, and raw to the mankind Cell colonization has anticancer function.Raffinose has Stabilization to the system containing high unsaturated oils, therefore can using Raffinose as Stabilizer adds the food grade emulsion containing high unsaturated materials to, and is provided without embedding form and for food, thus prolongs The shelf life of long products.

Folium Stevlae Rebaudianae is herbaceos perennial, and province area is visitd by the Oman originating in Paraguay northeast.Containing big in its leaf The natural sweetener of amount, so that Folium Stevlae Rebaudianae has huge economic worth.For many years, dry Stevia leaves is used as natural Sweeting agent, then in the whole world, multiple countries go through to be used as food additive for its extract stevioside (STV) and Lay born of the same parents' enlightening glycosides A (RA) Add agent.The U.S., respectively in 2008 and 2009, assert that the skilful compounds of highly purified stevioside is Generally Recognized as safe state.So far Till, Folium Stevlae Rebaudianae is found that more than 100 kinds of compounds, most notable is exactly stevioside compounds, particularly content is Abundant STV and RA.Additionally, Folium Stevlae Rebaudianae also has the Diterpene glucoside compounds of other low contents, according to being connected to C13 position The difference of upper sugar chain, these compounds can be divided into four classes.Non-glucoside compound in Folium Stevlae Rebaudianae is labdane diterpenes. Additionally, Folium Stevlae Rebaudianae also includes phenols, flavonoid, water soluble vitamins, phytosterol, triterpenes, volatile ingredient and biology Alkaloid compound.

Summary of the invention

The object of the invention is contemplated to make up the defect of prior art, it is provided that the Chinese prescription of one group for the treatment of hyperthyroidism.

The present invention is achieved by the following technical solutions:

The Chinese prescription of one group for the treatment of hyperthyroidism, it is made up of following component and weight portion thereof:

Fresh Cymbidium ensifolium (L.) Sw. root stem 30-35, fresh Folium Stevlae Rebaudianae 50-60, Spica Prunellae 10-12, Galla Chinensis 10-12, Radix Adenophorae (Radix Glehniae) 13-15, Sargassum 12- 15, field winter 13-15,13-15 Radix Ophiopogonis, Fructus Citri tangerinae core 13-15, Radix Rehmanniae 13-15, pollen 10-12, Thallus Laminariae (Thallus Eckloniae) 10-12, Gekko Swinhonis 10-12, Rhizoma Curcumae 10-12, Herba Scutellariae Barbatae 10-12, Fructus Polygoni Orientalis 10-12, Bufo siccus 10-12, fresh Rhizoma dioscoreae 50-60.

The Chinese prescription of one group of described treatment hyperthyroidism, is made up of the following step:

1), respectively by Spica Prunellae, Galla Chinensis, Radix Adenophorae (Radix Glehniae), Sargassum, Tian Dong, Radix Ophiopogonis, Fructus Citri tangerinae core, the Radix Rehmanniae, Thallus Laminariae (Thallus Eckloniae), Gekko Swinhonis, Rhizoma Curcumae, half Lotus, Fructus Polygoni Orientalis, Bufo siccus are pulverized, and add the ethanol of 3-5 times of 60-70% after mixing, adjust pH9.0-10.0, reflux, extract, 2-3 time, often Secondary extraction 1.5-3 hour, collects extracting solution respectively and residue is standby；

2), by gained mixing residue in step 1, add the ethanol of 3-5 times amount 70-95%, adjust pH5.0-6.0, reflux, extract, 2-4 Secondary, extract 1.5-2.5 hour every time, collect extracting solution standby；

3), by step 1 gained extracting solution by centrifugation, filter after supernatant, reclaim after ethanol, regulate pH4.0-7.0, then through from The heart, filtration, rinse removing impurity, 70-90% ethanol elution respectively with pure water, 10-30% ethanol after macroporous resin adsorption, collect Eluent, obtains concentrated solution standby after concentration；

4), by extracting solution in step 2 by centrifugation, filter after supernatant, reclaim after ethanol, redissolve, decolouring, neutralize solution in It is centrifuged after property, is filtered to remove tannin, take supernatant, regulate pH10-12, after macroporous resin adsorption, with pure water, 10-30% ethanol Rinse respectively and remove impurity, 70-90% ethanol elution, collect eluent, be concentrated to give concentrated solution standby；

5), fresh Cymbidium ensifolium (L.) Sw. root stem, fresh Folium Stevlae Rebaudianae, fresh Rhizoma dioscoreae are rinsed well after, homogenate, mistake are shredded and be squeezed into drip except excessive moisture, After filtering slag with pollen, step 3, prepare concentrated solution in 4 and mix, vacuum dried prepare, be distributed into bag, every bag 50-55 gram, warm Mixing in water for oral taking, every day one bag.

The invention have the advantage that

1, the Chinese prescription of one group for the treatment of hyperthyroidism of the present invention, filters after fresh Cymbidium ensifolium (L.) Sw. root stem, fresh Folium Stevlae Rebaudianae, fresh Rhizoma dioscoreae are rushed fresh squeezing Slagging-off, leaves and takes the beneficiating ingredient that tissue is fine and smooth, is vacuum dried after mixing with Chinese medicine extract, as a kind of carrier mass, To improve the ratio of concentrated solution solid content, effective ingredient is played package action, and dried powder can be increased as carrier The resistance to water soak of shape finished product and storing stability.

2, the Chinese prescription of one group for the treatment of hyperthyroidism of the present invention, containing substantial amounts of natural sweetener in its leaf of Folium Stevlae Rebaudianae, Improve the mouthfeel that Chinese medicine is pained；In addition, Folium Stevlae Rebaudianae also includes phenols, flavonoid, water soluble vitamins, phytosterol, three Terpenoid, volatile ingredient and alkaloid compound, strengthen health-care effect.

3, the Chinese prescription of one group for the treatment of hyperthyroidism of the present invention, in the rhizome of contained Herba Lycopi, content of stachyose is high, Herba Lycopi Oligosaccharide is mainly made up of stachyose, verbascose and Raffinose.Stachyose is as emerging functional oligose, and it is delaying Old and feeble, regulation micro ecology of gastrointestinal tract balance, the immunity that improves, toxin expelling, lowering blood pressure aspect have splendid physiological function；Cotton Son sugar has improvement result to atopic dermatitis, it is possible to as the active component of hepatopathy inhibitor, also have anti-blood Proteinaceous, anti- Sick effect, and Human germ cells is had anticancer function.

4, the Chinese prescription of one group for the treatment of hyperthyroidism of the present invention, Bufo siccus has the merit of heat clearing away, toxin expelling, antitumor, defying age Effect；It is equipped with Herba Scutellariae Barbatae, Spica Prunellae, Radix Ophiopogonis, there is inducing diuresis to remove edema, yin nourishing regulating menstruation, anticancer function.

Detailed description of the invention

The Chinese prescription of one group for the treatment of hyperthyroidism, it is made up of following component and weight portion thereof:

Fresh Cymbidium ensifolium (L.) Sw. root stem 30, fresh Folium Stevlae Rebaudianae 50, Spica Prunellae 10, Galla Chinensis 10, Radix Adenophorae (Radix Glehniae) 13, Sargassum 12, field winter 13, Radix Ophiopogonis 13, Fructus Citri tangerinae Core 13, the Radix Rehmanniae 13, pollen 10, Thallus Laminariae (Thallus Eckloniae) 10, Gekko Swinhonis 10, Rhizoma Curcumae 10, Herba Scutellariae Barbatae 10, Fructus Polygoni Orientalis 10, Bufo siccus 10, fresh Rhizoma dioscoreae 50.

The Chinese prescription of one group of described treatment hyperthyroidism, is made up of the following step:

1), respectively by Spica Prunellae, Galla Chinensis, Radix Adenophorae (Radix Glehniae), Sargassum, Tian Dong, Radix Ophiopogonis, Fructus Citri tangerinae core, the Radix Rehmanniae, Thallus Laminariae (Thallus Eckloniae), Gekko Swinhonis, Rhizoma Curcumae, half Lotus, Fructus Polygoni Orientalis, Bufo siccus are pulverized, and add the ethanol of 3 times 60% after mixing, adjust pH9.0, reflux, extract, 2 times, extract 1.5 little every time Time, collect extracting solution respectively and residue is standby；

2), by gained mixing residue in step 1, add the ethanol of 3 times amount 70%, adjust pH5.0, reflux, extract, 2 times, extract every time 1.5 hours, collect extracting solution standby；

3), by step 1 gained extracting solution by centrifugation, filter after supernatant, reclaim after ethanol, regulate pH4.0, more by centrifugation, Filter, after macroporous resin adsorption, rinse removing impurity, 70% ethanol elution respectively with pure water, 10% ethanol, collect eluent, dense Concentrated solution is obtained standby after contracting；

4), by extracting solution in step 2 by centrifugation, filter after supernatant, reclaim after ethanol, redissolve, decolouring, neutralize solution in It is centrifuged after property, is filtered to remove tannin, take supernatant, regulate pH10, after macroporous resin adsorption, rush respectively with pure water, 10% ethanol Eccysis decontamination, 70% ethanol elution, collect eluent, be concentrated to give concentrated solution standby；

5), fresh Cymbidium ensifolium (L.) Sw. root stem, fresh Folium Stevlae Rebaudianae, fresh Rhizoma dioscoreae are rinsed well after, homogenate, mistake are shredded and be squeezed into drip except excessive moisture, After filtering slag with pollen, step 3, prepare concentrated solution in 4 and mix, vacuum dried prepared, be distributed into bag, 50 grams every bag, warm water Take after mixing it with water, every day one bag.

Claims (2)

1. the Chinese prescription of one group for the treatment of hyperthyroidism, it is characterised in that it is made up of following component and weight portion thereof:

Fresh Cymbidium ensifolium (L.) Sw. root stem 30-35, fresh Folium Stevlae Rebaudianae 50-60, Spica Prunellae 10-12, Galla Chinensis 10-12, Radix Adenophorae (Radix Glehniae) 13-15, Sargassum 12- 15, field winter 13-15,13-15 Radix Ophiopogonis, Fructus Citri tangerinae core 13-15, Radix Rehmanniae 13-15, pollen 10-12, Thallus Laminariae (Thallus Eckloniae) 10-12, Gekko Swinhonis 10-12, Rhizoma Curcumae 10-12, Herba Scutellariae Barbatae 10-12, Fructus Polygoni Orientalis 10-12, Bufo siccus 10-12, fresh Rhizoma dioscoreae 50-60.

The Chinese prescription of one group for the treatment of hyperthyroidism the most according to claim 1, it is characterised in that be made up of the following step:

1), respectively by Spica Prunellae, Galla Chinensis, Radix Adenophorae (Radix Glehniae), Sargassum, Tian Dong, Radix Ophiopogonis, Fructus Citri tangerinae core, the Radix Rehmanniae, Thallus Laminariae (Thallus Eckloniae), Gekko Swinhonis, Rhizoma Curcumae, half Lotus, Fructus Polygoni Orientalis, Bufo siccus are pulverized, and add the ethanol of 3-5 times of 60-70% after mixing, adjust pH9.0-10.0, reflux, extract, 2-3 time, often Secondary extraction 1.5-3 hour, collects extracting solution respectively and residue is standby；

2), by gained mixing residue in step 1, add the ethanol of 3-5 times amount 70-95%, adjust pH5.0-6.0, reflux, extract, 2-4 Secondary, extract 1.5-2.5 hour every time, collect extracting solution standby；

3), by step 1 gained extracting solution by centrifugation, filter after supernatant, reclaim after ethanol, regulate pH4.0-7.0, then through from The heart, filtration, rinse removing impurity, 70-90% ethanol elution respectively with pure water, 10-30% ethanol after macroporous resin adsorption, collect Eluent, obtains concentrated solution standby after concentration；

4), by extracting solution in step 2 by centrifugation, filter after supernatant, reclaim after ethanol, redissolve, decolouring, neutralize solution in It is centrifuged after property, is filtered to remove tannin, take supernatant, regulate pH10-12, after macroporous resin adsorption, with pure water, 10-30% ethanol Rinse respectively and remove impurity, 70-90% ethanol elution, collect eluent, be concentrated to give concentrated solution standby；

5), fresh Cymbidium ensifolium (L.) Sw. root stem, fresh Folium Stevlae Rebaudianae, fresh Rhizoma dioscoreae are rinsed well after, homogenate, mistake are shredded and be squeezed into drip except excessive moisture, After filtering slag with pollen, step 3, prepare concentrated solution in 4 and mix, vacuum dried prepare, be distributed into bag, every bag 50-55 gram, warm Mixing in water for oral taking, every day one bag.