CN106177170A - Fructus Lycii crop is used to extract the preparation method of Fructus Lycii flavone - Google Patents
Fructus Lycii crop is used to extract the preparation method of Fructus Lycii flavone Download PDFInfo
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- CN106177170A CN106177170A CN201610611439.7A CN201610611439A CN106177170A CN 106177170 A CN106177170 A CN 106177170A CN 201610611439 A CN201610611439 A CN 201610611439A CN 106177170 A CN106177170 A CN 106177170A
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- fructus lycii
- flavone
- lycii
- skin zone
- folium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses the preparation method using Fructus Lycii crop to extract Fructus Lycii flavone, comprise the following steps: the root of wild barbarum plant is removed, stay Fructus Lycii bar portion and Folium Lycii, Fructus Lycii bar portion is removed the peel, obtain Fructus Lycii skin zone, by Folium Lycii after manually picking remove impurity, dry in the shade at indoor natural ventilating together with Fructus Lycii skin zone, obtain Folium Lycii dry sample and Fructus Lycii skin zone dry sample；By Folium Lycii dry sample and Fructus Lycii skin zone dry sample after crushed, cross 80 ~ 280 mesh sieves, obtain Folium Lycii powder and Fructus Lycii skin zone powder；Use extraction, absorption, eluting, concentration technique that Folium Lycii powder and Fructus Lycii skin zone powder are carried out Folium Lycii flavone and the preparation of Fructus Lycii skin zone flavone, obtain Folium Lycii flavone concentrated solution and Fructus Lycii skin zone flavone concentrated solution；Carry out Folium Lycii flavone concentrated solution and Fructus Lycii skin zone flavone concentrated solution mixing, being dried to obtain flavone powder, bar skin and the Folium Lycii of Fructus Lycii plant are carried out the preparation of flavone as raw material, the mode using the technology such as extraction, absorption, eluting, concentration to combine.
Description
Technical field
The present invention relates to natural product fabricating technology field, specifically, be to use Fructus Lycii crop to extract Fructus Lycii Huang
The preparation method of ketone.
Background technology
Fructus Lycii is that people are to the general designation of species under the Lyciums such as commodity Fructus Lycii, plant lycium barbarum, China Fructus Lycii.People
Daily edible and medicinal Fructus Lycii mostly is the fruit " Fructus Lycii " of lycium barbarum, and lycium barbarum is unique loading " 2010
Year version Chinese Pharmacopoeia " kind, with fine jade treasure Ganoderma, Changbai Mountain Radix Ginseng, Donga Colla Corii Asini referred to as Chinese medicine four treasured.
Flavone compound is referred to two phenyl ring (A-with B-ring) with phenolic hydroxyl group and is interconnected mutually by central authorities' thricarbon atom
A series of compounds of knot, its basic parent nucleus is 2-phenyl chromone.Flavone compound structure often connects and has phenol hydroxyl
The functional groups such as base, methoxyl group, methyl, isopentene group.Additionally, it is the most normal and sugar is combined into glycosides.
The discovery history of flavone compound is the longest.As far back as the beginning of the thirties in twentyth century, one Pharmaceutical Chemist in Europe
A kind of white crystals is obtained unintentionally, by its named " Citrin " when studying the ethanol extraction of Fericarpium Citri Limoniae.Animal experiment
Confirm: the Vitamin C effect of Citrin surpasses vitamin C 10 times.After 2 years, this scientist is further discovered that: Citrin is real
On border it is a kind of mixture being made up of flavone rather than one matter, therefore Citrin is renamed as lemon with visualizing by someone later
Lemon element.Studying according to descendant, citrin contains multiple flavone, and its key component is Hesperidin.This finds the science of Citrin the earliest
Family won Nobel chemistry Prize after 8 years.Regrettably, the medicinal study of the flavone including citrin does not has reality the most all the time
Matter is in progress.
Natural flavone compounds is many to be existed with glycoside form, and due to kind, quantity, coupled position and the connection of sugar
The mode difference of connecing can form various flavonoid glycoside.The saccharide of composition flavonoid glycoside includes monosaccharide and disaccharide, trisaccharide and is acylated
Sugar.Flavonoid glycoside solid is amorphous powder, and remaining flavone compound mostly is crystalline solid.The face that flavone compound is different
Color is that natural pigment family with the addition of more multicolour.This is owing to forming cross conjugation system in its parent nucleus, and is turned by electronics
Move, reset, make conjugated chain extend, thus show color.It is molten that flavonoid glycoside is the most soluble in water, ethanol, methanol isopolarity are strong
In agent；But it is insoluble in or insoluble in the organic solvent such as benzene, chloroform.Sugar chain the most water-soluble the longest degree is the biggest.Flavone compound because of
Molecule has phenolic hydroxyl group more, thus aobvious acid.Acidity because of phenolic hydroxyl group number, position and different.
Flavone is widely present in certain plants and the berry of nature, and sum about kind more than 4,000, its molecular structure is not to the utmost
Identical, all belong to flavone such as rutin, hesperidin, quercimentin, green tea polyphenol, pattern glucosides, pattern thuja acid etc..Different molecular structures
Flavone may act on the organ that health is different, such as Semen Ginkgo Fructus Crataegi--cardiovascular system, and Lan Mei--eyes, tart fruit--urinary tract system,
Fructus Vitis viniferae--lymph, liver, fruit of Ramulus Sambuci Williamsii--immune system, at ordinary times we can pass through polyphagia Fructus Vitis viniferae, Bulbus Allii Cepae, Brassica oleracea var. botrytis L., drink red
The modes such as wine, polydipsia green tea obtain flavone, and the one as health is supplemented.Before 20 years, scientist finds have living fossil
Title ginkgo in containing relatively large number of flavone, from Folium Ginkgo, mainly extract flavone.
Summary of the invention
It is an object of the invention to provide the preparation method using Fructus Lycii crop to extract Fructus Lycii flavone, by the bar of Fructus Lycii plant
Skin and Folium Lycii carry out the preparation of flavone as raw material, the mode using the technology such as extraction, absorption, eluting, concentration to combine,
There is with low cost, extraction efficiency high.
The present invention is achieved through the following technical solutions: use Fructus Lycii crop extract Fructus Lycii flavone preparation method, including with
Lower step:
1) root of wild barbarum plant is removed, stays Fructus Lycii bar portion and Folium Lycii, Fructus Lycii bar portion is removed the peel, obtains Fructus Lycii skin zone,
By Folium Lycii after manually picking remove impurity, dry in the shade at indoor natural ventilating together with Fructus Lycii skin zone, obtain Folium Lycii dry sample and Chinese holly
Qi skin zone dry sample；
2) by Folium Lycii dry sample after crushed, cross 80 ~ 280 mesh sieves, obtain Folium Lycii powder；
3), after Fructus Lycii skin zone dry sample being pulverized, cross 80 ~ 280 mesh sieves, obtain Fructus Lycii skin zone powder；
4) use extraction, absorption, eluting, concentration technique that Folium Lycii powder carries out the preparation of Folium Lycii flavone, obtain Folium Lycii flavone
Concentrated solution；
5) use extraction, absorption, eluting, concentration technique that Fructus Lycii skin zone powder carries out the preparation of Fructus Lycii skin zone flavone, obtain Chinese wolfberry skin
Portion's flavone concentrated solution；
6) carry out Folium Lycii flavone concentrated solution and Fructus Lycii skin zone flavone concentrated solution mixing, being dried to obtain flavone powder.
The most to better implement the present invention, following set-up mode is used especially: described step 4) includes following tool
Body step:
4-1) by ethanol that Folium Lycii powder and mass concentration are 40 ~ 97.50% by 1: 40 ~ 90 material liquid volume than extraction 3 ~ 300h
After, obtain leaf portion lixiviating solution；
4-2) described leaf portion lixiviating solution is after colloid mill grinds 8 ~ 12 times, obtains the Folium Lycii extracting solution of colloidal；
After 4-3) the Folium Lycii extracting solution of described colloidal being carried out homogenizing under the pressure of 20 ~ 180Mpa, it is performing centrifugal separation on,
Obtain leaf portion supernatant and leaf portion filtering residue；
4-4) by described leaf portion supernatant with the ceramic membrane that flow velocity via hole diameter is 0.05 ~ 5 μm of 2 ~ 8t/h in temperature for 30 ~ 45
DEG C, pressure be remove impurity under conditions of 0.1 ~ 2Mpa, obtain leaf portion filtrate；
4-5) described leaf portion filtrate is after neutral macroporous adsorbent resin or alkalescence absorption with macroporous adsorbent resin, first uses deionized water
It is eluted to sugar-free, then adsorbs in macropore tree with the acetone soln eluting that 15 ~ 25 times of bed volume, mass concentration are 30% ~ 80%
Folium Lycii flavone on fat, obtains the eluent containing Folium Lycii flavone；
4-6) by the described eluent containing Folium Lycii flavone after vacuum-concentrcted, obtain Folium Lycii flavone concentrated solution.
The most to better implement the present invention, following set-up mode is used especially: described step 5) includes following tool
Body step:
5-1) by acetone that Fructus Lycii skin zone powder and mass concentration are 60 ~ 85% by 1: 50 ~ 80 material liquid volume than extraction 30 ~ 280h
After, obtain skin zone's lixiviating solution；
5-2) described skin zone lixiviating solution is after colloid mill grinds 6 ~ 12 times, obtains the Chinese wolfberry skin extracting solution of colloidal；
After 5-3) the Chinese wolfberry skin extracting solution of described colloidal being carried out homogenizing under the pressure of 20 ~ 180Mpa, it is performing centrifugal separation on,
Obtain skin zone's supernatant and skin zone's filtering residue；
5-4) by described skin zone supernatant with the ceramic membrane that flow velocity via hole diameter is 0.05 ~ 5 μm of 2 ~ 8t/h in temperature for 25 ~ 45
DEG C, pressure be remove impurity under conditions of 0.4 ~ 1.2Mpa, obtain skin zone's filtrate；
5-5) described skin zone filtrate is after neutral macroporous adsorbent resin or alkalescence absorption with macroporous adsorbent resin, first uses deionized water
It is eluted to sugar-free, then with the acetone soln that 18 ~ 22 times of bed volume, mass concentration are 22% ~ 78% or methanol solution or ethanol
Eluant solution absorption Fructus Lycii skin zone flavone on macroporous resin, obtains the eluent containing Fructus Lycii skin zone flavone；
5-6) by the described eluent containing Fructus Lycii skin zone flavone after vacuum-concentrcted, obtain Fructus Lycii skin zone flavone and concentrate
Liquid.
The most to better implement the present invention, following set-up mode is used especially: described leaf portion lixiviating solution or employing
Prepared by following preparation method: by ethanol that described Folium Lycii powder and mass concentration are 40 ~ 97.50% by 1: 10 ~ 80 feed liquid body
Long-pending ratio carries out heating in water bath at a temperature of 37.5 ~ 80 DEG C, filters and get final product after reflux, extract, 2 ~ 64h.
The most to better implement the present invention, following set-up mode is used especially: described leaf portion lixiviating solution or employing
Prepared by following preparation method: by ethanol that described Folium Lycii powder and mass concentration are 40 ~ 97.50% by 1: 10 ~ 80 feed liquid body
Long-pending ratio carries out supersound extraction at a temperature of 37.5 ~ 80 DEG C, filters and get final product after 8 ~ 48h.
Further the most to better implement the present invention, use following set-up mode especially: described step 4-2) in colloid
Rotor speed when mill grinds is 10000 ~ 180000r/min.
Further the most to better implement the present invention, use following set-up mode especially: described step 4-3) in centrifugal point
From time centrifugal speed be 8000 ~ 12000r/min, centrifugation time is 1 ~ 60min.
Further the most to better implement the present invention, use following set-up mode especially: described step 4-6) in vacuum
The condition of concentrating under reduced pressure be vacuum be 0.011 ~ 0.028MPa, temperature is 30 ~ 50 DEG C.
The present invention compared with prior art, has the following advantages and beneficial effect:
The bar skin of Fructus Lycii plant and Folium Lycii as raw material, are used the technology phases such as extraction, absorption, eluting, concentration by the present invention
In conjunction with mode carry out the preparation of flavone, there is with low cost, extraction efficiency high.
The present invention, by colloid mill break-in technology, high pressure homogenization technique and membrane separation technique use in conjunction, the most only effectively contracts
Short extraction time, decrease the Extraction solvent consumption in technical process and energy consumption, and gained flavone stable in properties, content
Height, the biological activity such as resisting cardiovascular disease, anticancer, antioxidation is high.
The present invention uses Ceramic Membranes Separating Technique, the most effectively removes the tannin in extracting solution, phlegmatic temperament, antibacterial etc. miscellaneous
Matter, and significantly reduce equipment investment, reduce energy consumption, also make process simplification simultaneously.
The present invention uses colloid mill and high pressure homogenizer, not only ensure that the effective ingredient of Folium Lycii and Fructus Lycii bar skin zone
Homogeneous, stable in properties, and gained flavone resisting cardiovascular disease, the anticancer and active height of antioxidative, and can contract significantly
Short extraction time and raising extraction ratio.
Instrument and equipment of the present invention is easy and simple to handle, and technical parameter is easily controllable, and wide accommodation can be used for industrialization extensive
Produce, form biological product industrial chain.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1:
Use Fructus Lycii crop to extract the preparation method of Fructus Lycii flavone, using the bar skin of Fructus Lycii plant and Folium Lycii as raw material, adopt
The mode combined by technology such as extraction, absorption, eluting, concentrations carries out the preparation of flavone, have with low cost, extraction efficiency is high
Etc. feature, use following set-up mode especially: comprise the following steps:
1) root of wild barbarum plant is removed, stays Fructus Lycii bar portion and Folium Lycii, Fructus Lycii bar portion is removed the peel, obtains Fructus Lycii skin zone,
By Folium Lycii after manually picking remove impurity, dry in the shade at indoor natural ventilating together with Fructus Lycii skin zone, obtain Folium Lycii dry sample and Chinese holly
Qi skin zone dry sample；
2) by Folium Lycii dry sample after crushed, cross 80 ~ 280 mesh sieves, obtain Folium Lycii powder；
3), after Fructus Lycii skin zone dry sample being pulverized, cross 80 ~ 280 mesh sieves, obtain Fructus Lycii skin zone powder；
4) use extraction, absorption, eluting, concentration technique that Folium Lycii powder carries out the preparation of Folium Lycii flavone, obtain Folium Lycii flavone
Concentrated solution；
5) use extraction, absorption, eluting, concentration technique that Fructus Lycii skin zone powder carries out the preparation of Fructus Lycii skin zone flavone, obtain Chinese wolfberry skin
Portion's flavone concentrated solution；
6) carry out Folium Lycii flavone concentrated solution and Fructus Lycii skin zone flavone concentrated solution mixing, being dried to obtain flavone powder.
Embodiment 2:
The present embodiment is to optimize further on the basis of above-described embodiment, the most to better implement the present invention, especially
Use following set-up mode: described step 4) includes step in detail below:
4-1) by ethanol that Folium Lycii powder and mass concentration are 40 ~ 97.50% by 1: 40 ~ 90 material liquid volume than extraction 3 ~ 300h
After, obtain leaf portion lixiviating solution；
4-2) described leaf portion lixiviating solution is after colloid mill grinds 8 ~ 12 times, obtains the Folium Lycii extracting solution of colloidal；
After 4-3) the Folium Lycii extracting solution of described colloidal being carried out homogenizing under the pressure of 20 ~ 180Mpa, it is performing centrifugal separation on,
Obtain leaf portion supernatant and leaf portion filtering residue；
4-4) by described leaf portion supernatant with the ceramic membrane that flow velocity via hole diameter is 0.05 ~ 5 μm of 2 ~ 8t/h in temperature for 30 ~ 45
DEG C, pressure be remove impurity under conditions of 0.1 ~ 2Mpa, obtain leaf portion filtrate；
4-5) described leaf portion filtrate is after neutral macroporous adsorbent resin or alkalescence absorption with macroporous adsorbent resin, first uses deionized water
It is eluted to sugar-free, then adsorbs in macropore tree with the acetone soln eluting that 15 ~ 25 times of bed volume, mass concentration are 30% ~ 80%
Folium Lycii flavone on fat, obtains the eluent containing Folium Lycii flavone；
4-6) by the described eluent containing Folium Lycii flavone after vacuum-concentrcted, obtain Folium Lycii flavone concentrated solution.
Embodiment 3:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, the most to better implement the present invention,
Use especially following set-up mode: described step 5) includes step in detail below:
5-1) by acetone that Fructus Lycii skin zone powder and mass concentration are 60 ~ 85% by 1: 50 ~ 80 material liquid volume than extraction 30 ~ 280h
After, obtain skin zone's lixiviating solution；
5-2) described skin zone lixiviating solution is after colloid mill grinds 6 ~ 12 times, obtains the Chinese wolfberry skin extracting solution of colloidal；
After 5-3) the Chinese wolfberry skin extracting solution of described colloidal being carried out homogenizing under the pressure of 20 ~ 180Mpa, it is performing centrifugal separation on,
Obtain skin zone's supernatant and skin zone's filtering residue；
5-4) by described skin zone supernatant with the ceramic membrane that flow velocity via hole diameter is 0.05 ~ 5 μm of 2 ~ 8t/h in temperature for 25 ~ 45
DEG C, pressure be remove impurity under conditions of 0.4 ~ 1.2Mpa, obtain skin zone's filtrate；
5-5) described skin zone filtrate is after neutral macroporous adsorbent resin or alkalescence absorption with macroporous adsorbent resin, first uses deionized water
It is eluted to sugar-free, then with the acetone soln that 18 ~ 22 times of bed volume, mass concentration are 22% ~ 78% or methanol solution or ethanol
Eluant solution absorption Fructus Lycii skin zone flavone on macroporous resin, obtains the eluent containing Fructus Lycii skin zone flavone；
5-6) by the described eluent containing Fructus Lycii skin zone flavone after vacuum-concentrcted, obtain Fructus Lycii skin zone flavone and concentrate
Liquid.
Embodiment 4:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, the most to better implement the present invention,
Use especially following set-up mode: described leaf portion lixiviating solution or use following preparation method to prepare: by described Folium Lycii powder and matter
Measure the ethanol that concentration is 40 ~ 97.50% and carry out heating in water bath by the material liquid volume of 1: 10 ~ 80 than at a temperature of 37.5 ~ 80 DEG C, warp
Filter after reflux, extract, 2 ~ 64h and get final product.
Embodiment 5:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, the most to better implement the present invention,
Use especially following set-up mode: described leaf portion lixiviating solution or use following preparation method to prepare: by described Folium Lycii powder and matter
Amount concentration be 40 ~ 97.50% ethanol carry out supersound extraction than at a temperature of 37.5 ~ 80 DEG C by the material liquid volume of 1: 10 ~ 80,8 ~
Filter after 48h and get final product.
Embodiment 6:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, the most to better implement the present invention,
Use especially following set-up mode: described step 4-2) in colloid mill rotor speed when grinding be 10000 ~ 180000r/
min。
Embodiment 7:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, the most to better implement the present invention,
Use especially following set-up mode: described step 4-3) in centrifugation time centrifugal speed be 8000 ~ 12000r/min, centrifugal
Time is 1 ~ 60min.
Embodiment 8:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, the most to better implement the present invention,
Use especially following set-up mode: described step 4-6) in the condition of vacuum-concentrcted be vacuum be 0.011 ~
0.028MPa, temperature is 30 ~ 50 DEG C.
The above, be only presently preferred embodiments of the present invention, and the present invention not does any pro forma restriction, every depends on
Any simple modification of being made above example according to the technical spirit of the present invention, equivalent variations, each fall within the protection of the present invention
Within the scope of.
Claims (8)
1. use Fructus Lycii crop to extract the preparation method of Fructus Lycii flavone, it is characterised in that: comprise the following steps:
1) root of wild barbarum plant is removed, stays Fructus Lycii bar portion and Folium Lycii, Fructus Lycii bar portion is removed the peel, obtains Fructus Lycii skin zone,
By Folium Lycii after manually picking remove impurity, dry in the shade at indoor natural ventilating together with Fructus Lycii skin zone, obtain Folium Lycii dry sample and Chinese holly
Qi skin zone dry sample；
2) by Folium Lycii dry sample after crushed, cross 80 ~ 280 mesh sieves, obtain Folium Lycii powder；
3), after Fructus Lycii skin zone dry sample being pulverized, cross 80 ~ 280 mesh sieves, obtain Fructus Lycii skin zone powder；
4) use extraction, absorption, eluting, concentration technique that Folium Lycii powder carries out the preparation of Folium Lycii flavone, obtain Folium Lycii flavone
Concentrated solution；
5) use extraction, absorption, eluting, concentration technique that Fructus Lycii skin zone powder carries out the preparation of Fructus Lycii skin zone flavone, obtain Chinese wolfberry skin
Portion's flavone concentrated solution；
6) carry out Folium Lycii flavone concentrated solution and Fructus Lycii skin zone flavone concentrated solution mixing, being dried to obtain flavone powder.
The preparation method using Fructus Lycii crop to extract Fructus Lycii flavone the most according to claim 1, it is characterised in that: described step
Rapid 4) step in detail below is included:
4-1) by ethanol that Folium Lycii powder and mass concentration are 40 ~ 97.50% by 1: 40 ~ 90 material liquid volume than extraction 3 ~ 300h
After, obtain leaf portion lixiviating solution；
4-2) described leaf portion lixiviating solution is after colloid mill grinds 8 ~ 12 times, obtains the Folium Lycii extracting solution of colloidal；
After 4-3) the Folium Lycii extracting solution of described colloidal being carried out homogenizing under the pressure of 20 ~ 180Mpa, it is performing centrifugal separation on,
Obtain leaf portion supernatant and leaf portion filtering residue；
4-4) by described leaf portion supernatant with the ceramic membrane that flow velocity via hole diameter is 0.05 ~ 5 μm of 2 ~ 8t/h in temperature for 30 ~ 45
DEG C, pressure be remove impurity under conditions of 0.1 ~ 2Mpa, obtain leaf portion filtrate；
4-5) described leaf portion filtrate is after neutral macroporous adsorbent resin or alkalescence absorption with macroporous adsorbent resin, first uses deionized water
It is eluted to sugar-free, then adsorbs in macropore tree with the acetone soln eluting that 15 ~ 25 times of bed volume, mass concentration are 30% ~ 80%
Folium Lycii flavone on fat, obtains the eluent containing Folium Lycii flavone；
4-6) by the described eluent containing Folium Lycii flavone after vacuum-concentrcted, obtain Folium Lycii flavone concentrated solution.
The preparation method using Fructus Lycii crop to extract Fructus Lycii flavone the most according to claim 1, it is characterised in that: described step
Rapid 5) step in detail below is included:
5-1) by acetone that Fructus Lycii skin zone powder and mass concentration are 60 ~ 85% by 1: 50 ~ 80 material liquid volume than extraction 30 ~ 280h
After, obtain skin zone's lixiviating solution；
5-2) described skin zone lixiviating solution is after colloid mill grinds 6 ~ 12 times, obtains the Chinese wolfberry skin extracting solution of colloidal；
After 5-3) the Chinese wolfberry skin extracting solution of described colloidal being carried out homogenizing under the pressure of 20 ~ 180Mpa, it is performing centrifugal separation on,
Obtain skin zone's supernatant and skin zone's filtering residue；
5-4) by described skin zone supernatant with the ceramic membrane that flow velocity via hole diameter is 0.05 ~ 5 μm of 2 ~ 8t/h in temperature for 25 ~ 45
DEG C, pressure be remove impurity under conditions of 0.4 ~ 1.2Mpa, obtain skin zone's filtrate；
5-5) described skin zone filtrate is after neutral macroporous adsorbent resin or alkalescence absorption with macroporous adsorbent resin, first uses deionized water
It is eluted to sugar-free, then with the acetone soln that 18 ~ 22 times of bed volume, mass concentration are 22% ~ 78% or methanol solution or ethanol
Eluant solution absorption Fructus Lycii skin zone flavone on macroporous resin, obtains the eluent containing Fructus Lycii skin zone flavone；
5-6) by the described eluent containing Fructus Lycii skin zone flavone after vacuum-concentrcted, obtain Fructus Lycii skin zone flavone and concentrate
Liquid.
The preparation method using Fructus Lycii crop to extract Fructus Lycii flavone the most according to claim 2, it is characterised in that: described leaf
Portion's lixiviating solution or use following preparation method to prepare: by described Folium Lycii powder and mass concentration be the ethanol of 40 ~ 97.50% by 1:
The material liquid volume of 10 ~ 80 carries out heating in water bath than at a temperature of 37.5 ~ 80 DEG C, filters and get final product after reflux, extract, 2 ~ 64h.
The preparation method using Fructus Lycii crop to extract Fructus Lycii flavone the most according to claim 2, it is characterised in that: described leaf
Portion's lixiviating solution or use following preparation method to prepare: by described Folium Lycii powder and mass concentration be the ethanol of 40 ~ 97.50% by 1:
The material liquid volume of 10 ~ 80 carries out supersound extraction than at a temperature of 37.5 ~ 80 DEG C, filters and get final product after 8 ~ 48h.
The preparation method using Fructus Lycii crop to extract Fructus Lycii flavone the most according to claim 2, it is characterised in that: described step
Rapid 4-2) in colloid mill rotor speed when grinding be 10000 ~ 180000r/min.
7. the preparation method using Fructus Lycii crop to extract Fructus Lycii flavone as claimed in claim 2, it is characterised in that: described step
In 4-3), centrifugal speed during centrifugation is 8000 ~ 12000r/min, and centrifugation time is 1 ~ 60min.
8. the preparation method using Fructus Lycii crop to extract Fructus Lycii flavone as claimed in claim 2, it is characterised in that: described step
The condition of the vacuum-concentrcted in 4-6) be vacuum be 0.011 ~ 0.028MPa, temperature is 30 ~ 50 DEG C.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991709A (en) * | 2010-11-09 | 2011-03-30 | 中国科学院西北高原生物研究所 | Method for preparing antioxidant lycium ruthenicum extract |
| CN102000208A (en) * | 2010-11-09 | 2011-04-06 | 中国科学院西北高原生物研究所 | Method for extracting and preparing medlar leaf flavone |
| CN102432480A (en) * | 2011-11-17 | 2012-05-02 | 中国科学院西北高原生物研究所 | Method for extracting lycine from Chinese wolfberry leaves |
-
2016
- 2016-07-30 CN CN201610611439.7A patent/CN106177170A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991709A (en) * | 2010-11-09 | 2011-03-30 | 中国科学院西北高原生物研究所 | Method for preparing antioxidant lycium ruthenicum extract |
| CN102000208A (en) * | 2010-11-09 | 2011-04-06 | 中国科学院西北高原生物研究所 | Method for extracting and preparing medlar leaf flavone |
| CN102432480A (en) * | 2011-11-17 | 2012-05-02 | 中国科学院西北高原生物研究所 | Method for extracting lycine from Chinese wolfberry leaves |
Non-Patent Citations (2)
| Title |
|---|
| 李丽娟等: "枸杞茎叶中黄酮类成分提取和含量分析", 《黑龙江科技信息》 * |
| 闫宏等: "枸杞生产加工废弃物中活性成分含量及免疫性能的测定", 《饲料工业》 * |
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