CN102210726A - Method for combined extraction of mallow flavones and mallow saponins in seashore mallow and application - Google Patents
Method for combined extraction of mallow flavones and mallow saponins in seashore mallow and application Download PDFInfo
- Publication number
- CN102210726A CN102210726A CN2011101574512A CN201110157451A CN102210726A CN 102210726 A CN102210726 A CN 102210726A CN 2011101574512 A CN2011101574512 A CN 2011101574512A CN 201110157451 A CN201110157451 A CN 201110157451A CN 102210726 A CN102210726 A CN 102210726A
- Authority
- CN
- China
- Prior art keywords
- radix malvae
- malvae sylvestris
- flavone
- saponin
- mallow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229930003944 flavone Natural products 0.000 title claims abstract description 71
- 235000011949 flavones Nutrition 0.000 title claims abstract description 71
- 229930182490 saponin Natural products 0.000 title claims abstract description 52
- 150000007949 saponins Chemical class 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000000605 extraction Methods 0.000 title claims abstract description 6
- 240000000982 Malva neglecta Species 0.000 title abstract 8
- 235000000060 Malva neglecta Nutrition 0.000 title abstract 8
- 150000002213 flavones Chemical class 0.000 title abstract 5
- 235000017709 saponins Nutrition 0.000 title abstract 5
- 241000341711 Kosteletzkya pentacarpos Species 0.000 title abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 50
- 239000000287 crude extract Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 7
- 239000011347 resin Substances 0.000 claims abstract description 7
- 229920005989 resin Polymers 0.000 claims abstract description 7
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 6
- 235000013402 health food Nutrition 0.000 claims abstract description 4
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims description 66
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 66
- 150000002212 flavone derivatives Chemical class 0.000 claims description 52
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims description 47
- 241000982152 Kosteletzkya virginica Species 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 14
- 238000010828 elution Methods 0.000 claims description 11
- 239000000706 filtrate Substances 0.000 claims description 7
- 239000003463 adsorbent Substances 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000002604 ultrasonography Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000011550 stock solution Substances 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 238000007796 conventional method Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 230000009467 reduction Effects 0.000 claims description 3
- 238000002390 rotary evaporation Methods 0.000 claims description 3
- 238000009834 vaporization Methods 0.000 claims description 3
- 230000008016 vaporization Effects 0.000 claims description 3
- 239000008176 lyophilized powder Substances 0.000 claims description 2
- 238000011321 prophylaxis Methods 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 238000011282 treatment Methods 0.000 claims description 2
- 239000003480 eluent Substances 0.000 abstract description 4
- 239000002775 capsule Substances 0.000 abstract description 2
- 238000001035 drying Methods 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- -1 flavone compound Chemical class 0.000 description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- 230000000694 effects Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 238000002835 absorbance Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 230000008827 biological function Effects 0.000 description 5
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 229930182486 flavonoid glycoside Natural products 0.000 description 4
- 150000007955 flavonoid glycosides Chemical class 0.000 description 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 235000013824 polyphenols Nutrition 0.000 description 4
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 4
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 4
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 4
- 235000012141 vanillin Nutrition 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 3
- 230000000259 anti-tumor effect Effects 0.000 description 3
- 229940093797 bioflavonoids Drugs 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 3
- 150000002338 glycosides Chemical class 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 3
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 3
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 3
- 235000005493 rutin Nutrition 0.000 description 3
- 229960004555 rutoside Drugs 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 150000008130 triterpenoid saponins Chemical class 0.000 description 3
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- HDPISCIDKUMRDI-UHFFFAOYSA-M N(=O)[O-].[Na+].[N+](=O)([O-])[O-].[Al+3] Chemical compound N(=O)[O-].[Na+].[N+](=O)([O-])[O-].[Al+3] HDPISCIDKUMRDI-UHFFFAOYSA-M 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 229930182470 glycoside Natural products 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000010288 sodium nitrite Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- YHQXBTXEYZIYOV-UHFFFAOYSA-N 3-methylbut-1-ene Chemical group CC(C)C=C YHQXBTXEYZIYOV-UHFFFAOYSA-N 0.000 description 1
- 241000208173 Apiaceae Species 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 102100034279 Calcium-binding mitochondrial carrier protein Aralar2 Human genes 0.000 description 1
- 108010001394 Disaccharidases Proteins 0.000 description 1
- 241001339235 Eremalche Species 0.000 description 1
- 241000218791 Ginkgoaceae Species 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 241001093501 Rutaceae Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 229940112822 chewing gum Drugs 0.000 description 1
- 108010084210 citrin Proteins 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000000527 lymphocytic effect Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 125000001424 substituent group Chemical class 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 150000004043 trisaccharides Chemical class 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for combined extraction of mallow flavones and mallow saponins in seashore mallow, which is characterized by comprising the following steps of: taking the root tuber of fresh seashore mallow, and obtaining dry powder; carrying out ultrasonic treatment; extracting with 70% ethanol; removing the ethanol, and concentrating to obtain crude extracts of flavones and saponins; separating with macroporous resin; washing with water; eluting with 40% ethanol to obtain an eluent containing mallow flavones; then, eluting with 80% ethanol to obtain an eluent containing mallow saponins; and respectively concentrating and drying the two eluents. The mallow flavones and mallow saponins prepared by the method disclosed by the invention can be used for preparing health foods for preventing tumors or medicaments for treating tumors, and can be respectively or jointly prepared into powder, tablets, oral solutions, capsules and other foods or medicaments.
Description
Technical field
The invention belongs to technical field of biochemical separation, particularly a kind of method of extracting Radix Malvae sylvestris flavone and Radix Malvae sylvestris saponin of uniting from Kosteletzkya virginica the invention still further relates to the Radix Malvae sylvestris flavone that preceding method extracts and the purposes of Radix Malvae sylvestris saponin.
Background technology
Kosteletzkya virginica (kostelezkya virginica) is the perennial perennial root halophytes of Malvaceae mallow, and natural distributed is coastal sabkha littoral zone in the eastern united states.Introduced a fine variety to China by Nanjing University in 1992.Bibliographical information is arranged, the flower of Kosteletzkya virginica, leaf, root edible.Especially root can be eaten raw, can fry, can make tea-drinking, dries to pulverize to can be used as confection and chewing-gum material, to increase adhesiveness.The root leachate can be concentrated into the Ovum Gallus domesticus album shape and substitute the Ovum Gallus domesticus album use.
Two of flavone compound general references have a series of chemical compounds that the phenyl ring of phenolic hydroxyl group links mutually by central thricarbon atom, often are connected with functional groups such as phenolic hydroxyl group, methoxyl group, methyl, isopentene group in the flavone compound structure.In addition, its also normal and sugar is combined into glycosides.
Bioflavonoids (bioflavonoids) also claim Citrin, normal and vitamin C is followed existence, and is extensive in distributed in nature, the platymiscium secondary metabolite, be one group of natural pigment that is present in vegetable, fruit, flower and the corn, because of being the yellow bioflavonoids that claims more.The natural flavone compounds is many to be existed with the glycoside form, and because kind, quantity, coupled position and the connecting mode difference of sugar can be formed various flavonoid glycoside.The saccharide of forming flavonoid glycoside comprises monosaccharide, disaccharidase, trisaccharide and acidylate sugar.The flavonoid glycoside solid is an amorphous powder, and all the other flavone compounds mostly are crystalline solid.In the solvent that flavonoid glycoside is generally soluble in water, ethanol, methanol isopolarity are strong; But be insoluble in or be insoluble in the organic solvents such as benzene, chloroform.The long more then water-soluble degree of sugar chain is big more.Flavone compound because of having phenolic hydroxyl group, so show acid more in the molecule.Acid power is different because of phenolic hydroxyl group number, position.Present known flavone compound monomer has kind more than 8 000, and majority has color, and most of and sugar are combined into glycoside and exist with the form of aglucon in plant, and small part exists with free form.It is to be parent nucleus with the flavone, is connected with a series of substituent groups as hydroxyl etc. simultaneously, and its basic framework has C6-C3-C6.Flavone is present in flowers and fruits, leaf, stem, skin and the root of plant mostly, is the secondary metabolites of plant, and it is more extensive to distribute in plant kingdom, mainly is distributed in Rutaceae, Labiatae, pulse family, Umbelliferae, Ginkgoaceae, Compositae etc.Flavone compound more and more causes gazing at of people with the broad-spectrum pharmacological action.
Flavone compound has the antioxidation ability and then is higher than vitamin C far away.
Flavone compound has antitumor action.
Flavone compound has antiinflammatory action.
Flavone compound has the effect of enhance immunity.
Flavone compound has the effect of protection cardiovascular organization.
Flavone compound has the effect of protection Liver and kidney.
Flavone compound has blood coagulation resisting function.
The preparation technology of Radix Malvae sylvestris saponin has had patent application, as application number is: 200910032019.3.But the technology of separation and Extraction Radix Malvae sylvestris flavone and Radix Malvae sylvestris saponin is not reported so far from Kosteletzkya virginica.
Summary of the invention
The problem that the present invention need solve is at the deficiencies in the prior art, provides a kind of new, technology reasonably to unite the method for extracting Radix Malvae sylvestris flavone and Radix Malvae sylvestris saponin from Kosteletzkya virginica.
The present invention also provides the Radix Malvae sylvestris flavone that said method makes, the purposes of Radix Malvae sylvestris saponin.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of method of extracting Radix Malvae sylvestris flavone and Radix Malvae sylvestris saponin of uniting from Kosteletzkya virginica, is characterized in: gather the bright tuber of Kosteletzkya virginica, clean, oven dry is pulverized, and adding weight ratio in dry powder is the distilled water of 1:15, uses the Ultrasound Instrument supersound extraction; Ultrasound condition is: 60 ℃ of temperature, and power 200W, 1 hour time, after the ultrasonic end, to filter, filtrate is deposited, and the above-mentioned condition of filtering residue reuse repeats to extract once, and twice filtrate merges, and reduction vaporization gets concentrated solution; Adding ethanol in concentrated solution, to make ultimate density be 70%; Left standstill after the stirring 10-12 hour, and got the supernatant concentrating under reduced pressure and remove ethanol, must contain the crude extract of Radix Malvae sylvestris flavone, Radix Malvae sylvestris saponin;
Get the crude extract that contains Radix Malvae sylvestris flavone, Radix Malvae sylvestris saponin, separate again, according to a conventional method macroporous adsorbent resin is handled, the dress post with macroporous adsorbent resin; To contain Radix Malvae sylvestris flavone then, the crude extract upper prop of Radix Malvae sylvestris saponin, behind the end of the sample, earlier with distilled water flush away impurity, use 40% ethanol elution Radix Malvae sylvestris flavone then, collection contains the ethanol elution of Radix Malvae sylvestris flavone, after the eluting Radix Malvae sylvestris flavone finishes, re-use 80% ethanol elution Radix Malvae sylvestris saponin, obtain containing the ethanol elution of Radix Malvae sylvestris saponin, with these two kinds of elute solns respectively rotary evaporation remove ethanol, obtain containing Radix Malvae sylvestris flavone respectively, the concentrated solution of Radix Malvae sylvestris saponin is handled two kinds of solution respectively as required, directly make raw material and use, or stock solution is made lyophilized powder deposit standby with stock solution.
The Radix Malvae sylvestris flavone that above-described the inventive method makes, the application of Radix Malvae sylvestris saponin in the medicine of health food for preparing prophylaxis of tumours or treatment tumor.
Below be that usefulness the inventive method that the inventor did is separated the Radix Malvae sylvestris flavone that makes, the biological function test and the result thereof of Radix Malvae sylvestris saponin.
1.1. the immunocompetence of Radix Malvae sylvestris flavone test
Table 1. flavone is to the lymphocytic in-vitro multiplication effect of mouse thymus (24h)
Table 2. Radix Malvae sylvestris flavone is to the in-vitro multiplication test (24h) of mouse spleen lymphocyte
1.2 Radix Malvae sylvestris flavone anti-tumor activity test
Table 3. flavone is to the inhibitory action (24h) of hep-s cell growth
2. the biological function of Radix Malvae sylvestris saponin test
2.1 the antitumor of Radix Malvae sylvestris saponin test
Table 4. Radix Malvae sylvestris saponin is to the influence of HEP-S cell proliferation
According to table 1,2, the Kosteletzkya virginica flavone has the effect of significant raising mouse immune cell proliferation; According to table 3 and 4, Kosteletzkya virginica flavone and Saponin have the effect of remarkable inhibition Hep-S growth of tumour cell again.
According to inventor's result of study proof Radix Malvae sylvestris flavone, Radix Malvae sylvestris saponin, content is higher in Radix Malvae sylvestris tuber portion.Prove that from biological experiment Radix Malvae sylvestris flavone, Radix Malvae sylvestris saponin all have good biological function.Can make powder according to the different biological function of two kinds of materials, tablet, capsule, oral liquid, injection as people's health food, medicine, play a role for promoting people health.
The beneficial effect of the inventive method is: it uses ultrasonic technique, unites for the first time to have extracted Radix Malvae sylvestris flavone, Radix Malvae sylvestris saponin from Kosteletzkya virginica.Analyze after testing, Radix Malvae sylvestris flavone is a Flavonoid substances, and Radix Malvae sylvestris saponin is a triterpenoid saponin.Radix Malvae sylvestris flavone, Radix Malvae sylvestris certain herbaceous plants with big flowers Saponin are used for the zooblast experiment in vitro, find to have enhance immunity, suppress the growth of tumour cell effect, these two kinds of materials can be made as corresponding medicine according to its biological function.
The specific embodiment
Below further describe concrete technical scheme of the present invention,, and do not constitute restriction its right so that those skilled in the art understands the present invention further.
Embodiment 1.A kind of method of extracting Radix Malvae sylvestris flavone and Radix Malvae sylvestris saponin of from Kosteletzkya virginica, uniting, its step is as follows:
1. gather the bright tuber of Kosteletzkya virginica, clean, oven dry is pulverized.Get dry powder 50 and restrain the adding distilled water 750ml of 1:15 in proportion, stir evenly, use the Ultrasound Instrument supersound extraction, ultrasound condition: 60 ℃ of temperature, power 200W, 1 hour time.After the ultrasonic end, use two-layer filtered through gauze.Filtrate is deposited, and the above-mentioned condition of filtering residue reuse repeats once.Twice filtrate merges, and gets filtrate 950ml, and reduction vaporization gets concentrated solution 350ml.Adding 95% ethanol 910ml, to make ultimate density be 70%.Left standstill 10 hours after stirring several times, supernatant is got in siphon, and supernatant (70% ethanol extract) concentrating under reduced pressure is removed ethanol, gets Radix Malvae sylvestris flavone, Saponin crude extract 165ml.
With Radix Malvae sylvestris flavone, Saponin crude extract 165ml, adding distil water 300ml separates with macroporous adsorbent resin again.According to a conventional method macroporous adsorbent resin is handled the dress post; Then with Radix Malvae sylvestris flavone, Saponin crude extract upper prop, behind the end of the sample, earlier with distilled water flush away impurity, use 40% ethanol elution then, obtain containing the eluent 315ml of Radix Malvae sylvestris flavone, and then use 80% ethanol elution, obtain containing the ethanol elution 435ml of Radix Malvae sylvestris saponin, with two kinds of solution respectively rotary evaporation remove ethanol, obtain containing the concentrated solution 32ml of Radix Malvae sylvestris flavone.The concentrated solution 35ml of Radix Malvae sylvestris saponin, lyophilizing respectively then, Radix Malvae sylvestris flavone obtains 0.36g.Through measuring content with sodium nitrite-aluminum nitrate method is 12.5%.Radix Malvae sylvestris saponin obtains 0.31g, and measuring its content with perchloric acid-vanillin method is 14.3%.
2. the mensuration of Radix Malvae sylvestris flavone: sodium nitrite-aluminum nitrate method
According to result of study, Radix Malvae sylvestris flavone is a Flavonoid substances, with sodium sulfite-aluminum nitrate and its reaction, can produce color reaction, at the 510nm place absorption maximum is arranged.Radix Malvae sylvestris flavone can utilize rutin as reference flavone standard curve, calculates content and purity.
Agents useful for same: 1) standard rutin (0.1mg/ml);
2) 50% methanol;
3) 5% sodium nitrite
4) 10% aluminum nitrate
Accurately draw 0.1mg/ml rutin titer 0.0,1.0,2.0,3.0,4.0 5.0ml is in vitro common, each pipe adds 5% sodium nitrite solution 0.3ml respectively, left standstill 5 minutes, and added 10% aluminum nitrate solution 0.3ml again, left standstill 5 minutes, add with 50% methanol again and end every pipe 10ml, leave standstill colour developing in 5 minutes.The 510nm place measures absorbance value.With concentration absorbance value is made standard curve.The Radix Malvae sylvestris flavone sample gets 1.0,2.0 with the sample liquid of method preparation 0.1mg-0.5mg/ml concentration, 5.0ml, and all the other operate same standard curve.Radix Malvae sylvestris flavone content with the absorbance value contrast standard curve calculation sample that determines.
2. the mensuration of Radix Malvae sylvestris saponin: perchloric acid-vanillin method
Analyze after deliberation, Radix Malvae sylvestris saponin is a triterpenoid saponin, can produce color reaction according to triterpenoid saponin with vanillin under the perchloric acid effect, at the 546nm place absorption maximum is arranged.Radix Malvae sylvestris saponin content can pass through reference Saponin standard substance curve, calculates content and purity.
Vanillin reagent: 4%; 4.0 the gram vanillin adds the dissolving of 100ml glacial acetic acid and gets final product.Accurately draw 1.0mg/ml Saponin titer 0.0,0.025,0.035,0.050,0.075,0.10ml in the tool plug in vitro,
Add methanol and supply 0.10ml, add 4% vanillin reagent 0.4ml respectively, add perchloric acid 0.8ml again, mixing, close plug places 60 ℃ of water-bath heating 15 minutes, takes out, and with cold water cooling 5 minutes, each pipe added glacial acetic acid 5.0ml again, shakes up, and leaves standstill 10 minutes immediately.The 546nm place measures absorbance value.With concentration absorbance value is made standard curve.The Radix Malvae sylvestris saponin sample gets 0.01,0.02 with the sample liquid of method preparation 1.0mg/ml concentration, and 0.05ml adds methanol to 0.10ml, and all the other operate same standard curve.Radix Malvae sylvestris saponin content with the absorbance value contrast standard curve calculation sample that determines.
Claims (2)
1. unite the method for extracting Radix Malvae sylvestris flavone and Radix Malvae sylvestris saponin for one kind from Kosteletzkya virginica, it is characterized in that: gather the bright tuber of Kosteletzkya virginica, clean, oven dry is pulverized, and adding weight ratio in dry powder is the distilled water of 1:15, uses the Ultrasound Instrument supersound extraction; Ultrasound condition is: 60 ℃ of temperature, and power 200W, 1 hour time, after the ultrasonic end, to filter, filtrate is deposited, and the above-mentioned condition of filtering residue reuse repeats to extract once, and twice filtrate merges, and reduction vaporization gets concentrated solution; Adding ethanol in concentrated solution, to make ultimate density be 70%; Left standstill after the stirring 10-12 hour, and got the supernatant concentrating under reduced pressure and remove ethanol, must contain the crude extract of Radix Malvae sylvestris flavone, Radix Malvae sylvestris saponin;
Get the crude extract that contains Radix Malvae sylvestris flavone, Radix Malvae sylvestris saponin, separate again, according to a conventional method macroporous adsorbent resin is handled, the dress post with macroporous adsorbent resin; To contain Radix Malvae sylvestris flavone then, the crude extract upper prop of Radix Malvae sylvestris saponin, behind the end of the sample, earlier with distilled water flush away impurity, use 40% ethanol elution Radix Malvae sylvestris flavone then, collection contains the ethanol elution of Radix Malvae sylvestris flavone, after the eluting Radix Malvae sylvestris flavone finishes, re-use 80% ethanol elution Radix Malvae sylvestris saponin, obtain containing the ethanol elution of Radix Malvae sylvestris saponin, with these two kinds of elute solns respectively rotary evaporation remove ethanol, obtain containing Radix Malvae sylvestris flavone respectively, the concentrated solution of Radix Malvae sylvestris saponin is handled two kinds of solution respectively as required, directly make raw material and use, or stock solution is made lyophilized powder deposit standby with stock solution.
2. the Radix Malvae sylvestris flavone that makes of the described method of claim 1, the Radix Malvae sylvestris saponin application in the medicine of the health food of preparation prophylaxis of tumours or treatment tumor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101574512A CN102210726A (en) | 2011-06-13 | 2011-06-13 | Method for combined extraction of mallow flavones and mallow saponins in seashore mallow and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101574512A CN102210726A (en) | 2011-06-13 | 2011-06-13 | Method for combined extraction of mallow flavones and mallow saponins in seashore mallow and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102210726A true CN102210726A (en) | 2011-10-12 |
Family
ID=44742411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101574512A Pending CN102210726A (en) | 2011-06-13 | 2011-06-13 | Method for combined extraction of mallow flavones and mallow saponins in seashore mallow and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102210726A (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102861112A (en) * | 2012-08-31 | 2013-01-09 | 江苏省中国科学院植物研究所 | Kosteletzkya virginica lignan extract and preparation method and application thereof |
| CN103540483A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Preparation method of fresh Kosteletzkya virginica root tuber wine |
| CN103540484A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Preparation method of Kosteletzkya virginica dry root tuber medicinal wine |
| CN103540487A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Fresh Kosteletzkya virginica root tuber medicinal wine |
| CN103540486A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Preparation method of fresh Kosteletzkya virginica root tuber medicinal wine |
| CN103540491A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Fresh Kosteletzkya virginica root tuber wine and preparation method thereof |
| CN103540489A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Preparation method of Kosteletzkya virginica dry root tuber wine |
| CN103540482A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Kosteletzkya virginica dry root tuber wine |
| CN103540485A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Application of kosteletzkya virginica earthnuts to preparation of wine |
| CN103540490A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Kosteletzkya virginica dry root tuber medicinal wine |
| CN103540492A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Kosteletzkya virginica dry root tuber wine and preparation method thereof |
| CN109091511A (en) * | 2018-07-18 | 2018-12-28 | 中华全国供销合作总社南京野生植物综合利用研究所 | A kind of Microwave Extraction technique of kostelezkya virginica root tuber flavones |
| CN115068394A (en) * | 2022-06-28 | 2022-09-20 | 中北大学 | Whitening toner, emulsion and cream of compound natural plant extract |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601699A (en) * | 2009-07-07 | 2009-12-16 | 南京大学 | The preparation technology of kostelezkya virginica saponin and purposes |
-
2011
- 2011-06-13 CN CN2011101574512A patent/CN102210726A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601699A (en) * | 2009-07-07 | 2009-12-16 | 南京大学 | The preparation technology of kostelezkya virginica saponin and purposes |
Non-Patent Citations (1)
| Title |
|---|
| 艾国等: "黄蜀葵花化学成分及药理活性的研究进展", 《中国成人医药教育论坛》, 31 December 2010 (2010-12-31), pages 223 - 231 * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103540489A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Preparation method of Kosteletzkya virginica dry root tuber wine |
| CN103540485A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Application of kosteletzkya virginica earthnuts to preparation of wine |
| CN103540484A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Preparation method of Kosteletzkya virginica dry root tuber medicinal wine |
| CN103540487A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Fresh Kosteletzkya virginica root tuber medicinal wine |
| CN103540486A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Preparation method of fresh Kosteletzkya virginica root tuber medicinal wine |
| CN103540491A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Fresh Kosteletzkya virginica root tuber wine and preparation method thereof |
| CN103540483A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Preparation method of fresh Kosteletzkya virginica root tuber wine |
| CN103540482A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Kosteletzkya virginica dry root tuber wine |
| CN103540492A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Kosteletzkya virginica dry root tuber wine and preparation method thereof |
| CN103540490A (en) * | 2012-07-14 | 2014-01-29 | 南京大学连云港高新技术研究院 | Kosteletzkya virginica dry root tuber medicinal wine |
| CN102861112A (en) * | 2012-08-31 | 2013-01-09 | 江苏省中国科学院植物研究所 | Kosteletzkya virginica lignan extract and preparation method and application thereof |
| CN102861112B (en) * | 2012-08-31 | 2014-12-10 | 江苏省中国科学院植物研究所 | Kosteletzkya virginica lignan extract and preparation method and application thereof |
| CN109091511A (en) * | 2018-07-18 | 2018-12-28 | 中华全国供销合作总社南京野生植物综合利用研究所 | A kind of Microwave Extraction technique of kostelezkya virginica root tuber flavones |
| CN115068394A (en) * | 2022-06-28 | 2022-09-20 | 中北大学 | Whitening toner, emulsion and cream of compound natural plant extract |
| CN115068394B (en) * | 2022-06-28 | 2023-09-19 | 中北大学 | Whitening toner, emulsion and face cream containing compound natural plant extracts |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102210726A (en) | Method for combined extraction of mallow flavones and mallow saponins in seashore mallow and application | |
| CN101787061B (en) | Application of Quzhazhigan in preparation of preparations for preventing and treating cardiac-cerebral ischemia diseases, and preparation method thereof | |
| CN101242850A (en) | Composition, function and use of xanthoceras sorbifolia extract and compound isolated from same, method for preparing same | |
| Yuan-Yuan et al. | Chemical constituents from Solanum torvum | |
| CN101899070A (en) | The preparation method of flavonoid glycoside in the compression leg sharp separation cake of camellia oleifera seeds in a kind of | |
| CN110051726A (en) | The preparation method and application of general flavone and total starches in a kind of Qingqian Willow leaf | |
| CN110627861B (en) | Anemarrhena steroid saponin compound and preparation method and application thereof | |
| CN102286056A (en) | Oleanolic acid derivative and preparation method thereof | |
| CN101830892B (en) | Method for separating glycoside chemical components from tibetan capillaris | |
| CN101880306A (en) | Stauntonia brachyanthera Hand-Mazz saponins components as well as preparation method and application thereof | |
| CN109010415B (en) | Petroleum ether mung bean extract and preparation method and application thereof | |
| CN101703669B (en) | Smilax china effective fractions and extraction as well as purification process thereof | |
| CN101601699B (en) | Kostelezkya virginica saponin preparation process and application | |
| CN103113438B (en) | A kind of method from Extracting Hesperidin from Orange Peel and Yellow Pigment in Tangerine Peel | |
| CN102670935B (en) | Method for extracting total saponins from allium chinense | |
| CN102382156A (en) | Preparation method of rehmannioside D standard substance | |
| CN110283055B (en) | Compound and preparation method and application thereof | |
| CN107722087B (en) | Gynostemma pentaphylla flavonoid compound, preparation method thereof and application thereof in antitumor drugs | |
| CN113577142A (en) | Yi medicine polygonum cuspidatum extract as well as preparation method and anticancer application thereof | |
| CN109091602B (en) | Effective component of semen allii tuberosi, extraction method and application thereof in preparing liver injury protection medicine | |
| CN107573315B (en) | Flavonoid compound in hawthorn as well as preparation method and application thereof | |
| CN115304616B (en) | Gastrodia elata active compound and preparation method and application thereof | |
| CN115521322B (en) | Isopentenyl flavone compound, and preparation method and application thereof | |
| CN110218154B (en) | Compound separated from mung bean and separation method and application thereof | |
| CN103665090B (en) | Ilex cornuta saponin compound, its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20111012 |