Background technology
The blood of the Ink vessel transfusing circulation in human body or animal body performs such as inferior several functions: will be by pulmonary
The oxygen sucked is transported to histiocyte, from tissue to pulmonary delivery carbon dioxide and be discharged into outside, and will be by
The nutrient that digestive tract absorbs is transported to internal organs or histiocyte, as the catabolite of tissue, and will be for
Material unnecessary for organism is transported to kidney and is discharged to external, the He Er that will secrete from endocrine gland
Cover (hormone) and be transported to effect organ, tissue, be uniformly dispersed body heat and keep body temperature regularly,
In addition, the antibacterial invaded in organism, foreign body etc. are destroyed, Non-toxic.
Though this blood also serves as to judge the leading indicator of various disease or health status, but in blood
The platelet rich in somatomedin (Growth factor) and leukocyte be to use with therapeutic purposes.
Blood comprises erythrocyte, leukocyte, platelet etc., and wherein, platelet is primarily present in blood plasma,
Blood plasma is divided into platelet rich plasma (Platelet Rich Plasma, PRP) layer and platelet poor plasma (Platelet
Poor Plasma, PPP) layer.
Be referred to as PRP (Platelet Rich Plasma: platelet concentrate blood plasma) rich in hematoblastic blood plasma
Be positioned at blood plasma relatively under position, comprise cytokine (cytokine), platelet derived growth because of
Son (Platelet Derived Growth Factor, PDGF), transforming growth factor-beta 1 (Transforming
Growth Factor-BETA1, TGF-BETA1), VEGF (Vascular Endothelial
GrowthFactor, VEGP) etc. somatomedin, illustrated in the data such as paper its to dermatosis and
Wound healing is the most effective.
It addition, the almost depletion of blood of referred to as PPP (Platelet Poor Plasma: platelet poor plasma) is little
The blood plasma of plate is used as autoblood implant or autologous plasma cosmetics.
Wherein, Platelet Concentrate (PRP) layer only accounts for about the 1% of gathered blood, and viscosity is relatively
High and be attached to erythrocyte, therefore, it is difficult to gather.If described Platelet Concentrate (PRP) is transplanted to
Affected part, the most inside the knee, ligament, muscle etc., then play the stem cell stimulated around and contribute to
The effect of Hemapoiesis, uses with therapeutic purposes the most always, but because its amount is few and is attached to erythrocyte
And be difficult to gather, if erythrocyte enters into the human body, then can cause the biggest misery, and also can be because of
This produces inflammation, so focusing on and gathering the technology removing erythrocytic Platelet Concentrate (PRP).
But, currently in order to extract Platelet Concentrate layer, have by centrifugal separation processes, a secondary
Centrifugal separation processes carries out the process concentrated, and described process is to have top, bottom being injected into by blood
Behind the lower liquid indoor of the first separation container of liquid chamber, the first separation container being injected with blood is entered
Centrifugation of row, promotes the leukocyte and buffy separated in a centrifugal separation processes
(Buffy coat) layer and make its grade move to top liquid chamber.
Hereafter, there is following process: serve leukocyte and the blood of portion's liquid chamber to secondary separation container sidesway
Heavy buffycoat, again carries out secondary centrifuging separation and makes its grade concentrate, thus extract Platelet Concentrate.
Now, there is following problem points: not only to a secondary separation centrifugation of the container sidesway warp let-off
Blood stratum disjunctum during produce loss, and a centrifugation operation, secondary centrifuging separate
Operation is relatively complicated and the activity duration is longer.
Summary of the invention
[problem that invention to be solved]
The present invention proposes to solve described various problem points, its object is to provide a kind of structure
The most improved for extracting the blood separation rich in hematoblastic somatomedin and extracting dress
Put: an available container carries out extracting and gathering the process of Platelet Concentrate, and simplifies centrifugal
Separation process, even if only passing through separation and the extraction that centrifugal separation processes also can realize Platelet Concentrate one time
Operation.
A further object of the present invention is the separation providing a kind of structure to realize separated blood easily
Hematoblastic separation in Ceng and the mode of extraction improved for extract rich in hematoblastic growth because of
The blood separation of son and extraction element.
A further object of the present invention is that providing a kind of structure to reclaim with available syringe extracts in a reservoir
The mode of most of leukocyte obtain dividing for extracting the blood rich in hematoblastic somatomedin of improvement
From and extraction element.
[solving the means of problem]
Possessing in order to reach the present invention of described purpose: main body tube, it forms the receipts of accommodating blood in bottom
Hold spatial portion, formed on the top in described receiving space portion and there is in the way of internal diameter is narrower than bottom inclination
Portion and the passage portion of upper end open, and it is formed with separated space portion, described separated space portion houses via institute
A stratum disjunctum part for the blood stating the centrifugation by described blood of passage portion and separate;
Slide block (slider), it is attached to described receiving space portion in the way of slidably (sliding),
The blood being forced through described centrifugation via described passage portion to side, described separated space portion and separate
Stratum disjunctum;And
Upper cap (cap), it is attached to the upper end of described main body tube in removably mode, in central authorities
The joint portion that upper end with described passage portion is combined when described main body tube is combined is formed at bottom, and has storage
Depositing portion, described reservoir is to be formed, at described slide block in the way of a side lower part prolongation of described joint portion
During rising, flow into for the Platelet Concentrate in the stratum disjunctum of blood and house in the stratum disjunctum of described blood
Platelet Concentrate;And
Described upper cap forms inlet, first-class outlet and second outlet, described injection at upper surface
Mouth is available for entering in order to the first syringe being injected described blood by described passage portion, and described first flows out
Mouth is available for extracting to outside the second syringe of the Platelet Concentrate being housed in described reservoir and enters, institute
State second outlet and be available for extracting to outside the 3rd syringe of the leukocyte being housed in described separated space portion
Enter,
Possessing guiding tube, described guiding tube is attached to described second outlet side, extends to described separation sky
Between portion bottom till, guide the pin (needle) of described 3rd syringe.
It addition, assembly of the invention: in described inlet, first-class outlet, second outlet combination can
The the first elastic rubber entered for each first syringe described, the second syringe, the pin of the 3rd syringe
Plug, the second rubber stopper, the 3rd rubber stopper.
It addition, assembly of the invention: described guiding tube comprises the major diameter being attached to described 3rd rubber stopper
Portion, by the minor diameter extended in the way of eccentric with described major diameter portion and by described major diameter portion with
The rake that minor diameter connects,
In the bottom of described minor diameter, the end of the pin being formed for described 3rd syringe is passed through
Notch.
It addition, assembly of the invention is also equipped with lower cover, described lower cover is combined under described main body tube
Portion, prevents described slide block from coming off, and is being centrally formed through hole, in order to be available for promoting described cunning to upside
Block and make its elevating mechanism risen pass in and out.
It addition, assembly of the invention: described upper cap is also equipped with separating plate, and described separating plate is with vertically
Prominent mode is formed at the bottom surface of described reservoir.
[effect of invention]
The present invention has the effect that.
First, it is internally formed, at main body tube, the separated space portion divided with middle passage portion as medium
And receiving space portion, the reservoir connected with passage portion when combining upper cap is arranged in the bottom of upper cap,
Therefore have the effect that the stratum disjunctum that can be separated and extract blood by a container, thus can prevent
Loss in transfer, and significantly shorten the activity duration.
Second, the present invention is to realize the separation of blood in a container by a centrifugal separation processes
And extract, the convenience therefore with operation improves, and the advantage that can improve the efficiency of operation.
3rd, though the degree of depth containing the separated space portion 104 of leukocyte is relatively deep, but the 3rd syringe 30
Pin 31 be directed into guiding tube 400, therefore can realize stable recovery.
4th, the separated space by rake 105a division receiving space portion 102 and separated space portion 104
The bottom in portion 104 is more towards the internal face side of main body tube 100, then space is the narrowest.In this case,
The end guiding the pin 31 in guiding tube 400 is positioned at the position corresponding with the mid portion of rake 105a
Put, therefore, it is difficult to extract the leukocyte of the foot remaining in the narrow separated space portion 104 of gradually stenosis,
But because being positioned at the minor diameter 402 of the lower side of guiding tube 400 and notch 402a in an eccentric manner and
The end of curved guide pin 31, thus the leukocyte of the foot remaining in separated space portion 104 can be extracted.
Detailed description of the invention
The present invention is carried out when containing blood in being formed at the receiving space portion of bottom of main body tube
Centrifugation, makes the leukocyte of the stratum disjunctum as the blood being performing centrifugal separation on be separated to the separation of main body tube
In spatial portion, in the way of connecting with the passage portion of main body tube, combine upper cap afterwards and only can to collect
And extraction Platelet Concentrate, thus can by a centrifugation and container carry out blood separation,
And the extraction operation of Platelet Concentrate and leukocyte.
Hereinafter, referring to the drawings, in detail the preferred embodiments of the present invention are illustrated.
If illustrated referring to figs. 1 through Fig. 5, then the present invention for extract rich in hematoblastic growth
Blood separation and the extraction element of the factor possess: main body tube 100, and it forms the receipts of accommodating blood in bottom
Hold spatial portion 102, formed in the way of internal diameter is narrower than bottom on the top in described receiving space portion 102
There is the passage portion 105 of rake 105a and upper end open, and be formed with separated space portion 104, described
Separated space portion 104 houses the centrifugation by described blood via described passage portion 105 and separates
Blood stratum disjunctum a part;Slide block 200, it is slidably coupled to described receiving space
Portion 102, is forced through described centrifugal point via described passage portion 105 to side, described separated space portion 104
From and the stratum disjunctum of blood that separates;And upper cap 300, it is attached to described master in removably mode
The upper end of body pipe 100, is formed at when described main body tube 100 is combined and described path in central lower
The joint portion 310 that the upper end in portion 105 combines, and there is reservoir 320, described reservoir 320 is with to institute
The mode of the side lower part prolongation stating joint portion 310 is formed, when described slide block 200 rises, for blood
Stratum disjunctum in Platelet Concentrate P flow into and house the Platelet Concentrate P in the stratum disjunctum of described blood.
Upper surface at described upper cap 300 is formed with inlet 300c, first-class outlet 300a and second
Flow export 300b, described inlet 300c are available in order to inject described blood by described passage portion 105
First syringe 10 enters, and described first-class outlet 300a is available for extracting to outside being housed in described storage
Second syringe 20 of the Platelet Concentrate P in portion 320 enters, and described second outlet 300b is available for
The 3rd syringe 30 extracting the leukocyte W being housed in described separated space portion 104 to outside enters.
Combining guiding tube 400 in described second outlet 300b side, described guiding tube 400 extends to described
Till the bottom in separated space portion 104, guide the pin 31 of described 3rd syringe 30.
It is available for described in described inlet 300c, first-class outlet 300a, second outlet 300b combination
Each first syringe, the second syringe, the pin (needle) of the 3rd syringe 10,20,30 enter
First rubber stopper of elasticity, the second rubber stopper, the 3rd rubber stopper 304,302,303.
If illustrated in more detail, then separated space portion 104 is in the upside of main body tube 100, with
Passage portion 105 divides, with receiving space portion 102, the structure come for medium, and in constructed as below:
When described slide block 200 rises, the stratum disjunctum one of the blood separated for use by the centrifugation of described blood
Part flows into and houses a stratum disjunctum part for described blood by described passage portion 105.
It addition, in the bottom of main body tube 100, combine lower cover 250 in removably mode, described under
Portion's lid 250 prevents described slide block 200 from coming off, and is formed with through hole 255, in order to be available for promoting to upside
Described slide block 200 and make it increase lifting tool (not shown) turnover.
Passage portion 105 is formed at the upper side in receiving space portion 102 in a vertical manner, is narrower than receipts with internal diameter
The mode of the internal diameter holding spatial portion 102 has rake 105a.
Thus, the separated space by rake 105a division receiving space portion 102 and separated space portion 104
The bottom in portion 104 is more towards the internal face side of main body tube 100, then the space housing leukocyte W is the narrowest.
The joint portion 310 of upper cap 300 is formed with the combined hole 312 of lower openings, in order to be engaged to lead to
The upper end outer peripheral face in road portion 105.
Reservoir 320 has in the way of the position that bottom face is positioned at less than the bottom of joint portion 310
The structure formed.
That is, reservoir 320 has the structure housing the Platelet Concentrate P flowed into by passage portion 105.
Slide block 200 comprises the first plunger member, the second plunger member 210,220.
First plunger member 210 combines sealing (sealing) parts of multiple annular state at outer peripheral face periphery,
Can to keep airtight, and reduce receiving space portion 102 when carrying out vertical motion with uniform pressure
Volume.
Second plunger member 220 is silicon (silicon) material, is combined in the bottom of described first plunger member 210,
When utilizing lifting tool to make the first plunger member 210 carry out vertical motion, the first plunger member 210 can be protected.
First plunger member 210 has constructed as below: by by lifting tool (cylinder (cylinder) especially
On actuator (actuator) or the finger of people) climbing power transmitted and carry out vertical motion, along main body tube
The inner peripheral surface in the receiving space portion 102 of 100 rises.
Now, lifting tool can enter into receiving space portion 102 by the through hole 255 of lower cover 250
Inside, gives unlifting pressure to slide block 200.
When slide block 200 carries out vertical motion, the extracted amount (such as, 1.0~2.5cc) of Platelet Concentrate P
Can change according to the change of height.
After collecting the Platelet Concentrate P of set volume, will be able to be extracted by the second syringe 20
Outside is extracted at the Platelet Concentrate P of described reservoir 320.
On the other hand, reservoir 320 and joint portion 310 can also be integral to a main body
The bottom surfaces of portion's lid 300, or the bottom of upper cap 300 it is attached in removably mode.
Fig. 8 and Fig. 9 represents another embodiment of assembly of the invention.
The present embodiment represents the modification of described guiding tube 400, and described guiding tube 400 has constructed as below:
Comprise the major diameter portion 401 of described 3rd rubber stopper 303 that is attached to, with inclined with described major diameter portion 401
Minor diameter 402 that the mode of the heart extends and by described major diameter portion 401 with minor diameter 402 even
The rake 403 connect, and it is formed for described 3rd syringe in the bottom of described minor diameter 402
The notch 402a that the end of the pin 31 of 30 is passed through.
If the effect to the present invention with this composition illustrates, as follows.
First, with reference to Fig. 5 a, the present invention is used for the extraction blood separation rich in hematoblastic somatomedin
And extraction element makes the first syringe 10 being filled with blood after the first rubber stopper 304 side is passed through, lead to
Cross passage portion 105 in the receiving space portion 102 of main body tube 100, inject blood, utilize centrifugal separator
It is centrifuged the blood being infused in receiving space portion 102 separating as Fig. 5 b.
The injection rate of blood can be injected according to 30~about 50cc.
Now, upper cap 300 keeps the state being combined in the top of main body tube 100, in order at centrifugal point
Hermetic separation spatial portion 104 during from.
If centrifugation terminates, then, as shown in Fig. 5 c before slide block 200 carries out lifting action, make
Joint portion 310 and passage portion 105 upwards side shifting in the way of being spaced apart.
If centrifugation terminates, it is little that the most described blood is divided into erythrocyte R, leukocyte W, deshydremia
Plate P, is sequentially separated into leukocyte W, Platelet Concentrate P, erythrocyte R according to difference of specific gravity from upside.
Secondly, if making lifting tool enter into through hole 255 inside of lower cover 250 and to upside pushing and withstanding
Slide block 200, then slide block 200 carries out vertical motion in the inside in receiving space portion 102 and reduces collecting blood
Volume in the receiving space portion 102 of liquid stratum disjunctum.
Slide block 200 makes the white blood being stored in the stratum disjunctum of the blood in receiving space portion 102 when rising
Ball W layer is injected in the separated space portion 104 of main body tube 100 by passage portion 105.
The vertical motion of described slide block 200 is the injection rising to leukocyte W in separated space portion 104
Till end.
Now, Platelet Concentrate P is positioned at the inside of passage portion 105, and erythrocyte R keeps being housed in collecting
The state of the bottom of spatial portion 102.
Hereafter, as fig 5d, if making upper cap 300 again carry out down maneuver and make joint portion
310 combine in the way of being engaged to the end of passage portion 105, then the inside in receiving space portion 102 is with upper
The reservoir 320 of portion's lid 300 combines in the way of communicating with each other.
As depicted in fig. 5e, if after the joint portion 310 of upper cap 300 is engaged to passage portion 105, make
Industry personnel make slide block 200 carry out secondary rising again with lifting tool, then be filled with the stratum disjunctum of blood
The volume in the described receiving space portion 102 of a part reduces further and Platelet Concentrate P passes through passage portion
The upper end of 105 is flowed into the inside of the reservoir 320 of upper cap 300.
The secondary vertical motion of slide block 200 proceeds to Platelet Concentrate P inflow knot in reservoir 320
Till bundle.
It is stored into the capacity of Platelet Concentrate P of reservoir 320 injection rate according to blood and the shape of blood
Matter and variable, substantially concentrate store 1.5~about 3.0cc.
If the inflow that Platelet Concentrate P is in reservoir 320 terminates, the most as shown in figure 5f, operation people
Member performs following operation: make the second syringe 20 outside by the second rubber stopper 302 of upper cap 300
Portion extracts the Platelet Concentrate (PRP) being stored in reservoir 320.
Secondly, as shown in fig. 5g, following operation is performed: make the 3rd syringe 30 by upper cap 300
The 3rd rubber stopper 303 and extract the leukocyte W that is stored in separated space portion 104 to outside.Now,
The pin 31 of the 3rd syringe 30 is directed into guiding tube 400 and can stably be directed to deeper the dividing of the degree of depth
From spatial portion 104.
On the other hand, Fig. 6 and Fig. 7 is the figure of another embodiment of the reservoir representing the present invention.
Another embodiment of the reservoir of the present invention is identical with the element of the front embodiment illustrated before,
But being also equipped with separating plate 330, described separating plate 330 is formed in the way of direction vertically upward is prominent
The bottom surface of reservoir 320, in order to the left side being divided in figure the inside of reservoir 320 and right side.
Separating plate 330 performs following function: even if the mistake caused because of the excessive vertical motion of slide block 200
Operation and Platelet Concentrate P and erythrocyte R are mixed in reservoir 320 with unsegregated state, it is possible to
Difference of specific gravity is utilized to make the stratum disjunctum of be mixed into blood divide each other in divided reservoir 320
From.
When slide block 200 exceedingly rises, there is following worry: be filled in receiving space portion 102
Platelet Concentrate P and erythrocyte R is flowed in reservoir 320 by passage portion 105.
In this case, divide even if Platelet Concentrate P is mixed into erythrocyte R by separating plate 33
In becoming the reservoir 320 in left and right space, it is possible to utilize the difference in specific gravity of Platelet Concentrate P and erythrocyte R
Different and in the reservoir 320 on left side and right side separate.
That is, if the Platelet Concentrate P being relatively lighter than erythrocyte R is flowed into the right side storage of separating plate 330
Depositing after in portion 320 makes erythrocyte R flow into, then cross the top of separating plate 330 and storage on the left of being flowed into
Deposit the interior volume in portion 320.
Platelet Concentrate P is separated to separating plate 330 benchmark in the reservoir 320 in left side, erythrocyte
R keeps separating the state in right side reservoir 320.
Hereafter, the extracting tools such as syringe are utilized to extract the process of the Platelet Concentrate P separated with before
The process illustrated is identical.
On the other hand, with reference to Fig. 5 g, rake 105a receiving space portion 102 and separated space portion are divided
104 and the bottom in separated space portion 104 more towards the internal face side of main body tube 100, then space is the narrowest.
In this case, as shown in fig. 5g, guide be positioned in the end of the pin 31 of guiding tube 400 with
The position that the mid portion of rake 105a is corresponding, remains in, therefore, it is difficult to extract, the separation that gradually stenosis is narrow
The leukocyte of the foot of spatial portion 104.
With reference to Fig. 8 and Fig. 9 of expression another embodiment of the present invention, the device of the present embodiment is by with partially
Heart mode is positioned at the minor diameter 402 of the lower side of guiding tube 400 and notch 402a and to main body tube
The internal face lateral bend of 100 guides the end of pin 31, therefore can extract and remain in separated space portion 104
The leukocyte of foot.
Therefore, the present invention for extracting rich in the blood separation of hematoblastic somatomedin and extraction element
Can by a container and centrifugal separation processes realize the separation that be carried out by the centrifugation of blood and
Concentrate operation, therefore there is the loss that can prevent producing during existing transfer, and improve operation
The effect of convenience.