CN106174396A - A kind of method utilizing animal blood to make seasoning bottom material of chafing dish - Google Patents

A kind of method utilizing animal blood to make seasoning bottom material of chafing dish Download PDF

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Publication number
CN106174396A
CN106174396A CN201610524965.XA CN201610524965A CN106174396A CN 106174396 A CN106174396 A CN 106174396A CN 201610524965 A CN201610524965 A CN 201610524965A CN 106174396 A CN106174396 A CN 106174396A
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China
Prior art keywords
parts
chafing dish
bottom material
animal blood
serum albumin
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CN201610524965.XA
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Chinese (zh)
Inventor
张崟
王卫
张佳敏
吉莉莉
熊伟
边杨
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Sichuan Laochuandong Food Co Ltd
Chengdu University
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Sichuan Laochuandong Food Co Ltd
Chengdu University
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Application filed by Sichuan Laochuandong Food Co Ltd, Chengdu University filed Critical Sichuan Laochuandong Food Co Ltd
Priority to CN201610524965.XA priority Critical patent/CN106174396A/en
Publication of CN106174396A publication Critical patent/CN106174396A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The invention discloses a kind of method utilizing animal blood to make seasoning bottom material of chafing dish, the method makes the proteolysis in serum be small peptide and aminoacid by utilizing protease, make its fragrance matter at high temperature preparing in bottom material of chafing dish with glucide generation Maillard reaction again, not only can obtain the bottom material of chafing dish of unique flavor, also can realize the zero-emission of serum, thus the treatment pressure of sewage of slaughtering enterprise can be alleviated, reduce environmental pollution.

Description

A kind of method utilizing animal blood to make seasoning bottom material of chafing dish
Technical field
The present invention relates to the manufacture method of a kind of bottom material of chafing dish, particularly relate to one and utilize animal blood to make seasoning chaffy dish The method of bed material, belongs to poultry by-product manufacture field.
Background technology
Animal blood is poultry processing byproducts, in the middle-size and small-size slaughterhouse of China, often animal blood is entered sewer, thus COD, BOD of causing sewage exceed standard.Along with going deep into of green economy theory, the sewage disposal of meat producing plant is required more to come by China The strictest.The production cost of many middle-size and small-size meat producing plants is controlled to bring heavy burden by this.Animal blood has the highest battalion Supporting and be worth, in countries such as Denmark, Germany, France, animal blood is often considered tonic, thus is usually used in sausage, pudding, bread etc. Food processing.Containing rich in protein, trace element and many physiology active substances in animal blood, as immunoglobulin, Haemachrome, superoxide dismutase, thrombin etc..By animal blood recycling, it is possible not only to make the battalion such as protein therein Foster material is obtained by, and can reduce the sewage disposal burden of meat producing plant.This is very for slaughtering enterprise Favourable.
At present, the hemoglobin in animal blood can be used for extracting haemachrome, and serum is commonly used for feedstuff or straight after being dried Connect as discharge of wastewater.Although so animal blood to be achieved partially recycled utilization, but owing to operating process is loaded down with trivial details, cause back Receive relatively costly.Thus much meat producing plant is reluctant to be for recycling and reuse animal blood.Therefore, the recovery of animal blood is reduced Utilize cost, improve utilization ratio, on the hemoglobin basis in extracting blood, serum is comprehensively utilized the most necessary. Containing part plasma protein, polypeptide, fat, carbohydrate, inorganic matter etc. in serum, protease is utilized to make protein therein Be hydrolyzed to small peptide and aminoacid, then make its at high temperature with glucide generation Maillard reaction, and then produce fragrance matter, Fragrance matter is used for the exploitation of bottom material of chafing dish again, the high value added utilization of animal blood can be realized.
Summary of the invention
It is an object of the invention to provide a kind of method utilizing animal blood to make seasoning bottom material of chafing dish, the method passes through blood Clear enzymolysis and Maillard reaction make the fragrance matter in bottom material of chafing dish, not only can obtain the bottom material of chafing dish of unique flavor, Also can realize the zero-emission of serum, thus reduce meat producing plant cost of sewage disposal.
What the present invention provided utilizes the method for animal blood making seasoning bottom material of chafing dish to comprise the steps:
(1) extraction of serum albumin: take animal blood, puts into collected after centrifugation supernatant in centrifuge by animal blood, by upper Clear liquid is dried to obtain serum albumin powder;
(2) enzymolysis of serum albumin: serum albumin powder is added 100mL phosphate buffer by 2g serum albumin powder and carries out molten Solving, be subsequently adding papain, enzymolysis 3~5.5h under conditions of temperature is 55~70 DEG C, pH4~5, enzyme dosage presses serum Egg albumen powder quality is calculated as 7000~9000U/g, enzyme denaturing after enzymolysis, filters to obtain serum albumin enzymolysis solution;
(3) batch mixing: take glycine, alanine, glutamic acid, glucose and xylose in mass ratio, join after being mixed In serum albumin enzymolysis solution, mixing, homogenizing obtain mixed liquor;Take cycloheptaamylose, be dissolved in water, prepare cycloheptaamylose after homogenizing Solution;
(4) Maillard reaction and being dried: mixed liquor and cycloheptaamylose solution are pumped into by different sample feeding pipes simultaneously In spray dryer, mixed liquor carries out Maillard reaction and is dried, and carries out inclusion with cycloheptaamylose simultaneously;
(5) packaging: collect after the powder cooling that step (4) prepares, obtain Natural meat flavor;
(6) bottom material of chafing dish makes: mixed with other adjuvant by Natural meat flavor, prepares seasoning bottom material of chafing dish.
Further, in step (1), the rotating speed of centrifuge is 3500~4200r/min, and centrifugation time is 10~20min.
Further, in step (2), enzyme-removal temperature is 85~100 DEG C, and the enzyme denaturing time is 20~30min.
Further, in step (3) glycine, alanine, glutamic acid, the mass ratio of glucose and xylose be (15~ 18): (15~18): (20~30): (2~6): (28~58).
Further, glycine, alanine, glutamic acid, the mixture of glucose and xylose press 100 grams of serum albumin enzymes The ratio solving liquid addition 3~6g mixture joins in serum albumin enzymolysis solution.
Further, in step (3), cycloheptaamylose weighs by the 1.5~2.2% of serum albumin opaque amount, and presses β-ring Shape the dextrin 1.0~1.2g 100mL that adds water dissolves.
Further, in step (3) mixed liquor and cycloheptaamylose solution all use 8000~10000r/min turn The lower homogenizing 10~18min of speed.
Further, the wind speed being spray-dried in step (4) is 70~90m/s, and inlet amount is 50~65mL/min, air intake Temperature is 100~125 DEG C.
Further, other adjuvant described in step (6) includes the component of following weight portion, and each component is fragrant with natural meat flavour The weight proportion of material is: Natural meat flavor 20~40 parts, Adeps Bovis seu Bubali 1200~1500 parts, salad oil 800~1000 parts, Pixian County bean Lobe 300~500 parts, Pericarpium Zanthoxyli 500~750 parts, Semen Sojae Preparatum 10~15 parts, crystal sugar 30~50 parts, paprika 70~100 parts, Fructus Amomi Rotundus 3~ 5 parts, Fructus Tsaoko 3~5 parts, Rhizoma Kaempferiae 3~5 parts, Flos Caryophylli 1~3 parts, Fructus Amomi 3~5 parts, Oleum Linderae 3~5 parts, Fructus Cumini Cymini 3~5 parts, Cortex cinnamomi japonici (Ramulus Cinnamomi) 3~ 5 parts, Pericarpium Citri Reticulatae 3~5 parts, Herba Cymbopogonis Citrari 3~8 parts, anistree 3~5 parts, Fructus Foeniculi 3~8 parts, Rhizoma et radix valerianae 3~5 parts.
Present invention also offers the seasoning bottom material of chafing dish prepared by said method.
The present invention is by utilizing protease to make the proteolysis in serum be small peptide and aminoacid, then makes it at high temperature Make the fragrance matter in bottom material of chafing dish with glucide generation Maillard reaction, not only can obtain at the bottom of the chaffy dish of unique flavor Material, also can realize the zero-emission of serum, thus reduce meat producing plant cost of sewage disposal.
Below in conjunction with embodiment, the present invention is described in further details, but present disclosure is not limited to that.
Detailed description of the invention
Embodiment 1
What the present embodiment provided utilizes the method for animal blood making seasoning bottom material of chafing dish (spicy) to comprise the steps:
(1) extraction of serum albumin: take animal blood, puts into animal blood in centrifuge, under the rotating speed of 3500r/min from Heart 18min, collects supernatant, supernatant is dried to obtain serum albumin powder;
(2) enzymolysis of serum albumin: serum albumin powder is added 100mL phosphate buffer by 2g serum albumin powder and carries out molten Solve, be subsequently adding papain, temperature be 55 DEG C, enzymolysis 3.5h under conditions of pH4.8, enzyme dosage presses serum albumin opaque Amount is calculated as 8000U/g, at 95 DEG C of enzyme denaturing 20min after enzymolysis, obtains serum albumin enzymolysis solution after filtration;
(3) batch mixing: 15:16:20:2:47 in mass ratio takes glycine, alanine, glutamic acid, glucose and xylose, by it After mixing by 3.5% joining in serum albumin enzymolysis solution (i.e. 100 grams serum albumin enzymolysis solutions add 3.5g glycine, third Propylhomoserin, glutamic acid, the mixture of glucose and xylose), and homogenizing 16min under the rotating speed of 8500r/min, obtain mixed liquor;Press The quality of cycloheptaamylose is that the 1.5% of serum albumin opaque amount weighs cycloheptaamylose, it is added by cycloheptaamylose 1.0g Water 100mL dissolves, and homogenizing 16min under the rotating speed of 8500r/min, prepares cycloheptaamylose solution;
(4) Maillard reaction and being dried: by above-mentioned mixed liquor and cycloheptaamylose solution by different sample feeding pipes simultaneously Pumping in spray dryer, mixed liquor carries out Maillard reaction under the hot wind action of drying machine and is dried, and simultaneously Carrying out inclusion with cycloheptaamylose, the hot blast wind speed of spray drying is 75m/s, and inlet amount is 50mL/min, and inlet temperature is 120 ℃;
(5) packaging: collect to obtain Natural meat flavor at once, by natural after the powder that step (4) is prepared is cooled to room temperature Meat flavour perfume room temperature storage;
(6) making of bottom material of chafing dish: mixed with other adjuvant by Natural meat flavor, prepares spicy bottom material of chafing dish, institute The weight proportion stating Natural meat flavor and other adjuvant is: Natural meat flavor 20g, Adeps Bovis seu Bubali 1500g, salad oil 1000g, Pi County Semen Sojae Preparatum 500g, Pericarpium Zanthoxyli 750g, Semen Sojae Preparatum 15g, crystal sugar 50g, paprika 100g, Fructus Amomi Rotundus 5g, Fructus Tsaoko 5g, Rhizoma Kaempferiae 5g, Flos Caryophylli 3g, sand Core 5g, Oleum Linderae 5g, Fructus Cumini Cymini 5g, Cortex cinnamomi japonici (Ramulus Cinnamomi) 5g, Pericarpium Citri Reticulatae 5g, Herba Cymbopogonis Citrari 8g, anistree 5g, Fructus Foeniculi 8g, Rhizoma et radix valerianae 5g.
Embodiment 2
What the present embodiment provided utilizes the method for animal blood making seasoning bottom material of chafing dish (Herba Alii fistulosi fragrance) to comprise the steps:
(1) extraction of serum albumin: take animal blood, puts into animal blood in centrifuge, under the rotating speed of 4200r/min from Heart 12min, collects supernatant, supernatant is dried to obtain serum albumin powder;
(2) enzymolysis of serum albumin: serum albumin powder is added 100mL phosphate buffer by 2g serum albumin powder and carries out molten Solve, be subsequently adding papain, temperature be 70 DEG C, enzymolysis 5h under conditions of pH 5.0, enzyme dosage presses serum albumin opaque Amount is calculated as 9000U/g, at 85 DEG C of enzyme denaturing 25min after enzymolysis, obtains serum albumin enzymolysis solution after filtration;
(3) batch mixing: 18:16:25:4:30 in mass ratio takes glycine, alanine, glutamic acid, glucose and xylose, by it After mixing by 5% joining in serum albumin enzymolysis solution (i.e. 100 grams serum albumin enzymolysis solutions add 5g glycine, alanine, Glutamic acid, the mixture of glucose and xylose), and homogenizing 10min under the rotating speed of 9000r/min, obtain mixed liquor;By β-ring-type The quality of dextrin is that the 2.0% of serum albumin opaque amount weighs cycloheptaamylose, it is added water 100mL by cycloheptaamylose 1.2g Dissolve, and homogenizing 10min under the rotating speed of 9000r/min, prepare cycloheptaamylose solution;
(4) Maillard reaction and being dried: by above-mentioned mixed liquor and cycloheptaamylose solution by different sample feeding pipes simultaneously Pumping in spray dryer, mixed liquor carries out Maillard reaction under the hot wind action of drying machine and is dried, and simultaneously Carrying out inclusion with cycloheptaamylose, the hot blast wind speed of spray drying is 85m/s, and inlet amount is 60mL/min, and inlet temperature is 135 ℃;
(5) packaging: collect to obtain Natural meat flavor at once, by natural after the powder that step (4) is prepared is cooled to room temperature Meat flavour perfume room temperature storage;
(6) making of bottom material of chafing dish: mixed with other adjuvant by Natural meat flavor, prepares Herba Alii fistulosi fragrance bottom material of chafing dish, institute The weight proportion stating Natural meat flavor and other adjuvant is: Natural meat flavor 40g, Adeps Bovis seu Bubali 1200g, salad oil 900g, Pi County Semen Sojae Preparatum 300g, Pericarpium Zanthoxyli 600g, Semen Sojae Preparatum 10g, crystal sugar 30g, paprika 70g, Fructus Amomi Rotundus 3g, Fructus Tsaoko 3g, Rhizoma Kaempferiae 3g, Flos Caryophylli 1g, sand Core 3g, Oleum Linderae 3g, Fructus Cumini Cymini 3g, Cortex cinnamomi japonici (Ramulus Cinnamomi) 3g, Pericarpium Citri Reticulatae 4g, Herba Cymbopogonis Citrari 3g, anistree 4g, Fructus Foeniculi 3g, Rhizoma et radix valerianae 4g, onion powder 50g, big Bulbus Allii powder 70g.

Claims (10)

1. one kind utilizes the method that animal blood makes seasoning bottom material of chafing dish, it is characterised in that comprise the steps:
(1) extraction of serum albumin: take animal blood, puts into collected after centrifugation supernatant in centrifuge, by supernatant by animal blood It is dried to obtain serum albumin powder;
(2) enzymolysis of serum albumin: serum albumin powder is added 100mL phosphate buffer by 2g serum albumin powder and dissolves, Being subsequently adding papain, enzymolysis 3~5.5h under conditions of temperature is 55~70 DEG C, pH4~5, enzyme dosage presses serum egg White lead quality is calculated as 7000~9000U/g, enzyme denaturing after enzymolysis, filters to obtain serum albumin enzymolysis solution;
(3) batch mixing: take glycine, alanine, glutamic acid, glucose and xylose in mass ratio, joins serum after being mixed In protein enzymatic hydrolyzate, mixing, homogenizing obtain mixed liquor;Take cycloheptaamylose, be dissolved in water, to prepare cycloheptaamylose after homogenizing molten Liquid;
(4) Maillard reaction and be dried: mixed liquor and cycloheptaamylose solution are pumped into spraying by different sample feeding pipes simultaneously In drying machine, mixed liquor carries out Maillard reaction and is dried, and carries out inclusion with cycloheptaamylose simultaneously;
(5) packaging: collect after the powder cooling that step (4) prepares, obtain Natural meat flavor;
(6) bottom material of chafing dish makes: mixed with other adjuvant by Natural meat flavor, prepares seasoning bottom material of chafing dish.
The method utilizing animal blood to make seasoning bottom material of chafing dish the most according to claim 1, it is characterised in that: step (1) The rotating speed of middle centrifuge is 3500~4200r/min, and centrifugation time is 10~20min.
The method utilizing animal blood to make seasoning bottom material of chafing dish the most according to claim 1, it is characterised in that: step (2) Middle enzyme-removal temperature is 85~100 DEG C, and the enzyme denaturing time is 20~30min.
The method utilizing animal blood to make seasoning bottom material of chafing dish the most according to claim 1, it is characterised in that: step (3) Middle glycine, alanine, glutamic acid, the mass ratio of glucose and xylose are (15~18): (15~18): (20~30): (2~ 6): (28~58).
The method utilizing animal blood to make seasoning bottom material of chafing dish the most according to claim 4, it is characterised in that: glycine, Alanine, glutamic acid, the mixture of glucose and xylose are added the ratio of 3~6g mixture in 100 grams of serum albumin enzymolysis solutions Join in serum albumin enzymolysis solution.
The method utilizing animal blood to make seasoning bottom material of chafing dish the most according to claim 1, it is characterised in that: step (3) Middle cycloheptaamylose weighs by the 1.5~2.2% of serum albumin opaque amount, and adds water 100mL by cycloheptaamylose 1.0~1.2g Dissolve.
The method utilizing animal blood to make seasoning bottom material of chafing dish the most according to claim 1, it is characterised in that: step (3) Middle mixed liquor and cycloheptaamylose solution all use homogenizing 10~18min under the rotating speed of 8000~10000r/min.
The method utilizing animal blood to make seasoning bottom material of chafing dish the most according to claim 1, it is characterised in that: step (4) The wind speed of middle spray drying is 70~90m/s, and inlet amount is 50~65mL/min, and inlet temperature is 120~145 DEG C.
The method utilizing animal blood to make seasoning bottom material of chafing dish the most according to claim 1, it is characterised in that: step (6) Described in other adjuvant include the component of following weight portion, each component with the weight proportion of Natural meat flavor is: natural meat flavour Spice 20~40 parts, Adeps Bovis seu Bubali 1200~1500 parts, salad oil 800~1000 parts, Pixian watercress 300~500 parts, Pericarpium Zanthoxyli 500~ 750 parts, Semen Sojae Preparatum 10~15 parts, crystal sugar 30~50 parts, paprika 70~100 parts, Fructus Amomi Rotundus 3~5 parts, Fructus Tsaoko 3~5 parts, Rhizoma Kaempferiae 3~ 5 parts, Flos Caryophylli 1~3 parts, Fructus Amomi 3~5 parts, Oleum Linderae 3~5 parts, Fructus Cumini Cymini 3~5 parts, Cortex cinnamomi japonici (Ramulus Cinnamomi) 3~5 parts, Pericarpium Citri Reticulatae 3~5 parts, Herba Cymbopogonis Citrari 3 ~8 parts, anistree 3~5 parts, Fructus Foeniculi 3~8 parts, Rhizoma et radix valerianae 3~5 parts.
10. the seasoning bottom material of chafing dish prepared by any one method of claim 1-9.
CN201610524965.XA 2016-07-05 2016-07-05 A kind of method utilizing animal blood to make seasoning bottom material of chafing dish Pending CN106174396A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942343A (en) * 2017-05-09 2017-07-14 西藏农牧学院 A kind of Chilled Meats natural biological freshness-preserving agent and its application
CN110692801A (en) * 2019-11-14 2020-01-17 合肥工业大学 Reconstituted plasma protein powder and preparation method thereof

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GB855350A (en) * 1956-12-27 1960-11-30 Unilever Ltd Flavouring substances and their preparation
CN101153312A (en) * 2007-09-07 2008-04-02 东北农业大学 Blood albumen with oxidation resistance liveness and method for preparing the same
CN103005361A (en) * 2012-12-20 2013-04-03 南昌大学 Method for applying duck blood Maillard reaction essences in ham sausages
CN103053993A (en) * 2012-12-06 2013-04-24 南昌大学 Method of preparing meat-flavor essence from duck meat processing wastes
CN104543927A (en) * 2014-12-20 2015-04-29 重庆都好生物科技有限公司 Preparation method of pig blood reactive meat-flavor seasoner
CN104878063A (en) * 2015-06-10 2015-09-02 广西盟展鳄鱼科技开发有限公司 Crocodilian serum protein with antineoplastic function and preparation method thereof
CN105011085A (en) * 2014-04-24 2015-11-04 苏州要德食业有限公司 Butter hotpot condiment formula

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB855350A (en) * 1956-12-27 1960-11-30 Unilever Ltd Flavouring substances and their preparation
CN101153312A (en) * 2007-09-07 2008-04-02 东北农业大学 Blood albumen with oxidation resistance liveness and method for preparing the same
CN103053993A (en) * 2012-12-06 2013-04-24 南昌大学 Method of preparing meat-flavor essence from duck meat processing wastes
CN103005361A (en) * 2012-12-20 2013-04-03 南昌大学 Method for applying duck blood Maillard reaction essences in ham sausages
CN105011085A (en) * 2014-04-24 2015-11-04 苏州要德食业有限公司 Butter hotpot condiment formula
CN104543927A (en) * 2014-12-20 2015-04-29 重庆都好生物科技有限公司 Preparation method of pig blood reactive meat-flavor seasoner
CN104878063A (en) * 2015-06-10 2015-09-02 广西盟展鳄鱼科技开发有限公司 Crocodilian serum protein with antineoplastic function and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942343A (en) * 2017-05-09 2017-07-14 西藏农牧学院 A kind of Chilled Meats natural biological freshness-preserving agent and its application
CN110692801A (en) * 2019-11-14 2020-01-17 合肥工业大学 Reconstituted plasma protein powder and preparation method thereof

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