CN101153312A - Blood albumen with oxidation resistance liveness and method for preparing the same - Google Patents
Blood albumen with oxidation resistance liveness and method for preparing the same Download PDFInfo
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- CN101153312A CN101153312A CNA200710072775XA CN200710072775A CN101153312A CN 101153312 A CN101153312 A CN 101153312A CN A200710072775X A CNA200710072775X A CN A200710072775XA CN 200710072775 A CN200710072775 A CN 200710072775A CN 101153312 A CN101153312 A CN 101153312A
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Abstract
The invention provides an albumin peptide with anti-oxidative activity and a method for preparing the same. A substrate of porcine albumin with density of 4 percent undergoes preheating for 10 minutes under 90 DEG C, and is added with alkali proteinase for hydrolysis, with the ratio between the enzyme and the substrate [E/S] as 2: 100-200, the hydrolysis is carried out under 45-55 DEG C with pH 7.5-8.5 for 3-5 hours to obtain the product. The invention has simple operation, and is more reliable and safer compared with the synthesized antioxidant, thereby being suitable for industrialized production, capable of increasing dramatically the utilization ratio of the resource of albumin, and increasing the comprehensive utilization ratio of the blood. The prepared anti-oxidative albumin peptide with the anti-oxidative function both in vivo and in vitro can be added to the foods as the antioxidant.
Description
(1) technical field
The present invention relates to a kind of antioxidant from natural food.Specifically a kind of serum protein with anti-oxidant activity.The invention still further relates to a kind of preparation method with serum protein of anti-oxidant activity.
(2) background technology
Animal blood is the main by product of butchering processing, and protein content is about about 18%, but but very low in the utilization ratio of China's blood, therefore causes the huge waste of protein resource and brings bigger environmental stress thereupon.At present, utilize different protein resource development function polypeptide to become the focus of research.
Porcine blood serum albumen has good antioxygenation, the normal operation of its protection life.Serum protein is an antioxidant main in the blood circulation, and according to bibliographical information, serum protein can protect cultured cells to make it avoid the injury of oxyradical; Serum protein can suppress the catalytic lipid oxidation of iron ion; In vivo test research then shows, the low-density lipoprotein (LDL) that serum protein has a protection people makes it avoid oxidation and prevents erythrocytic hemolytic action.
(3) summary of the invention
The object of the present invention is to provide a kind of equal Wheat Protein in vitro and in vivo, can be used as antioxidant and be added into serum protein in the food with anti-oxidant activity.The present invention also aims to provide a kind of preparation method with serum protein of anti-oxidant activity.
The object of the present invention is achieved like this:
It is that concentration is 4% porcine blood serum protein substrate, under 90 ℃ of temperature, carry out after 10 minutes the thermal treatment, adding Sumizyme MP is hydrolyzed, the ratio [E/S] of enzyme-to-substrate concentration is 2: 100-120, under the condition of 45-55 ℃ of temperature, pH value 7.5-8.5, the molecular weight mixed peptide between 550.23-1022.7 that obtained in hydrolysis 3-5 hour.Product of the present invention is to adopt such method to prepare: be 4% porcine blood serum protein substrate with concentration, under 90 ℃ of temperature, carry out after 10 minutes the thermal treatment, adding Sumizyme MP is hydrolyzed, the ratio [E/S] of enzyme-to-substrate concentration is 2: 100-120, under the condition of 45-55 ℃ of temperature, pH value 7.5-8.5, obtained product in hydrolysis 3-5 hour.
Degree of hydrolysis of the present invention (DH) can reach 18.85%; Porcine blood serum protein peptide after the hydrolysis shows good antioxidant activity.
The characteristic of hydrolysate of the present invention can be verified by the following method:
Antioxidation activity in vitro carries out in Yelkin TTS lipid oxidation system (being called for short the Lipsome oxidation system), Lipsome oxidation system compound method is: take by weighing 0.89472g Repone K, 0.0776g Histidine, thin up is to 100mL, transfer its pH value to 6.8, the soybean lecithin 1g that gets purity 20% again is dissolved in Repone K-Histidine buffered soln, makes the liposome of 2mg/mL.Get the liposome 5mL of 10 times of dilutions when carrying out anti-oxidant the test, add sample 1mL, and add 0.1mL 50mm Fecl
3Solution and 0.1mL 10mm xitix salts solution, 37 ℃ of water-bath 1h, the serum protein hydrolyzate is compared with unhydrolysed serum protein, and its TBARs (stream is for the veronal acid number) descends 44.97%, and peroxide value (POV value) descends 47.06%; Reducing power (FRAP value) improves 3.68 times, and the clearance rate of DPPH free radical (1, the 1-hexichol is for bitter taste acyl group free radical) is reached 60%; The serum protein peptide of molecular weight less than 3kDa separated, behind SephadexG-25 gel chromatography, SP-SephadexC-25 cationic exchange and RP-HPLC purifying, obtained one group of active higher anti-oxidation peptide, the unimodal of the higher peptide of activity will be obtained after its separation, it is 550.23 that mass spectrum (MS) measurement result is shown as molecular weight, 568.19, the mixed peptide that 656.18 and 1022.7 peptide section is formed; Zooperal result shows, after rat oral gavage handled one month, the serum protein peptide can significantly reduce rat blood serum total cholesterol level (TC) and triglyceride levels (TG) and mda (MDA) content, and the serum protein peptide can significantly improve the vigor of catalase (CAT), superoxide-dismutase (SOD) and Selenoperoxidase (GSH-Px) in the serum.
Preparation method of the present invention is simple to operate, compares safe and reliablely with synthetized oxidation preventive agent, is fit to suitability for industrialized production, can increase substantially the utilization ratio of serum protein resource, improves the level of blood comprehensive utilization.The equal in vitro and in vivo Wheat Protein of prepared serum protein anti-oxidation peptide can be used as antioxidant and is added in the food.
(4) embodiment
For example the present invention is done in more detail below and describes:
Porcine blood serum protein substrate concentration is 4%, at 90 ℃, after the thermal treatment of 10min, adds Sumizyme MP and is hydrolyzed, and the ratio [E/S] of enzyme-to-substrate concentration is 2: 100, and hydrolysis temperature is 50 ℃, control pH value 8.0, hydrolysis time 4h.
Claims (3)
1. serum protein with anti-oxidant activity, it is characterized in that: it is that concentration is 4% porcine blood serum protein substrate, under 90 ℃ of temperature, carry out after 10 minutes the thermal treatment, adding Sumizyme MP is hydrolyzed, the ratio of enzyme-to-substrate concentration is 2: 100-120, under the condition of 45-55 ℃ of temperature, pH value 7.5-8.5, the molecular weight mixed peptide between 550.23-1022.7 that obtained in hydrolysis 3-5 hour.
2. preparation method with serum protein of anti-oxidant activity, it is characterized in that: be 4% porcine blood serum protein substrate with concentration, under 90 ℃ of temperature, carry out after 10 minutes the thermal treatment, adding Sumizyme MP is hydrolyzed, the ratio of enzyme-to-substrate concentration is 2: 100-120, under the condition of 45-55 ℃ of temperature, pH value 7.5-8.5, obtained product in hydrolysis 3-5 hour.
3. the preparation method with serum protein of anti-oxidant activity according to claim 2, it is characterized in that: be 4% porcine blood serum protein substrate with concentration, under 90 ℃ of temperature, carry out after 10 minutes the thermal treatment, adding Sumizyme MP is hydrolyzed, the ratio of enzyme-to-substrate concentration is 2: 100, under the condition of 50 ℃ of temperature, pH value 8.0, hydrolysis obtained product in 4 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710072775XA CN101153312B (en) | 2007-09-07 | 2007-09-07 | Blood albumen with oxidation resistance liveness and method for preparing the same |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200710072775XA CN101153312B (en) | 2007-09-07 | 2007-09-07 | Blood albumen with oxidation resistance liveness and method for preparing the same |
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| Publication Number | Publication Date |
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| CN101153312A true CN101153312A (en) | 2008-04-02 |
| CN101153312B CN101153312B (en) | 2011-04-27 |
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| CN200710072775XA Expired - Fee Related CN101153312B (en) | 2007-09-07 | 2007-09-07 | Blood albumen with oxidation resistance liveness and method for preparing the same |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824458A (en) * | 2010-04-15 | 2010-09-08 | 江南大学 | Method for preparing high-efficiency antioxidant potato protein antioxidant peptide |
| CN102191304A (en) * | 2011-03-23 | 2011-09-21 | 连云港福润食品有限公司 | Porcine plasma antioxidant peptide |
| CN101608205B (en) * | 2009-06-19 | 2012-05-23 | 东北农业大学 | Preparation method of pig serum protein antioxidant peptide by adopting microbial fermentation and product thereof |
| CN102703553A (en) * | 2012-05-04 | 2012-10-03 | 东北农业大学 | Method for using polysaccharide stem grafting coupling technique to improving pig plasma protein peptide antioxidant activity |
| CN106174396A (en) * | 2016-07-05 | 2016-12-07 | 成都大学 | A kind of method utilizing animal blood to make seasoning bottom material of chafing dish |
-
2007
- 2007-09-07 CN CN200710072775XA patent/CN101153312B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101608205B (en) * | 2009-06-19 | 2012-05-23 | 东北农业大学 | Preparation method of pig serum protein antioxidant peptide by adopting microbial fermentation and product thereof |
| CN101824458A (en) * | 2010-04-15 | 2010-09-08 | 江南大学 | Method for preparing high-efficiency antioxidant potato protein antioxidant peptide |
| CN102191304A (en) * | 2011-03-23 | 2011-09-21 | 连云港福润食品有限公司 | Porcine plasma antioxidant peptide |
| CN102703553A (en) * | 2012-05-04 | 2012-10-03 | 东北农业大学 | Method for using polysaccharide stem grafting coupling technique to improving pig plasma protein peptide antioxidant activity |
| CN106174396A (en) * | 2016-07-05 | 2016-12-07 | 成都大学 | A kind of method utilizing animal blood to make seasoning bottom material of chafing dish |
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| Publication number | Publication date |
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| CN101153312B (en) | 2011-04-27 |
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Inventor after: Kong Baohua Inventor after: Liu Jian Inventor after: Diao Xinping Inventor after: Zhang Hongwei Inventor before: Kong Baohua Inventor before: Liu Jian |
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Free format text: CORRECT: INVENTOR; FROM: KONG BAOHUA LIU QIAN TO: KONG BAOHUA LIU QIAN DIAO XINPING ZHANG HONGWEI |
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