CN106173264A - Alpha Ketoglutarate application in terms of improving animal feed intake and body weight - Google Patents
Alpha Ketoglutarate application in terms of improving animal feed intake and body weight Download PDFInfo
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- CN106173264A CN106173264A CN201610535051.3A CN201610535051A CN106173264A CN 106173264 A CN106173264 A CN 106173264A CN 201610535051 A CN201610535051 A CN 201610535051A CN 106173264 A CN106173264 A CN 106173264A
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- animal
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/50—Feeding-stuffs specially adapted for particular animals for rodents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
Abstract
The present invention relates to herding and animal feeding field, specifically disclose alpha Ketoglutarate application in terms of improving animal feed intake and body weight, even if animal absorbs alpha Ketoglutarate by oral way, the daily dose preferably absorbing alpha Ketoglutarate is 0.05~1.58g/kg with the weight of animals ratio, it is possible to significantly improve feed intake and the body weight of animal.The present invention makes multiparous animal absorb alpha Ketoglutarate by the way of adding alpha Ketoglutarate in the drinking water of animal or feedstuff, the most on-demand interpolation can not only be accomplished, change dosage in time, and be capable of using on a large scale under mechanical feed feeds water system.A ketoglutaric acid used in the present invention can the most conveniently buied, and is applied to improve animal feed intake and body weight, it is possible to bring considerable economic benefit and good social benefit, improves plant and the income of cultivation self-employed entrepreneurs, promotes the development of aquaculture.
Description
Technical field
The present invention relates to herding and animal feeding field, specifically, relate to α-ketoglutaric acid and improving animal feed intake
With the application in terms of body weight.
Background technology
Improving the reproductive capacity of animal to greatest extent is one of target of pursuing of husbandry sector, is also to improve animal husbandry economy
The important channel of benefit.
China is populous nation, the biggest to the demand of meat product.Wherein, Carnis Sus domestica is the main meat of China's consumption of resident
Class.But owing to by environment, nutrition, heredity, factor stress be waited to be affected, the live pig breeding stock of China and the amount of delivering for sale the most urgently carry
High.At present, China's year live pig amount of delivering for sale is about 600,000,000, and sow breeding stock is minimum at 41,000,000, and an average sow goes out
Column number 15.Increase the live pig amount of delivering for sale, improve growing and fattening pigs the speed of growth and deliver for sale body weight be solve this production problem important
Thinking and approach.Therefore, seek a kind of raising animal feed intake, accelerate method great popularization meaning on producing that its body weight increases
Justice, is applicable not only to pig, it is possible to be generalized to Other Meat product consumption animal, the such as domestic animal such as sheep, cattle.
A-ketoglutaric acid (α-Ketoglutaric acid, α-KG) is very important cellular energy metabolism intermediate product,
It and aminoacid, sodium, potassium, calcium, magnesium etc. becomes salt can form series of products, can be organic as the composition of sport nutrition beverage
Intermediate, the matched reagent of biochemical reagents.There are some researches show, α-ketoglutaric acid can alleviate piglet diarrhea, promotes that body weight increases
Adding and skeleton development, improve insulin in blood plasma, Insulin-like growth factor II-1 growth, promote pancreas beta-secretase insulin, improving should
Swash the level of Bone Gla protein under state, improve negative nitrogen balance and the muscle alienation etc. of postoperative patient.In the recent period, scholar is had to find, necessarily
The α-ketoglutaric acid of concentration can extend the life-span of nematicide;Daily ration adds α-ketoglutaric acid can promote the growth promoter of broiler chicken
With histoorgan performance.
Summary of the invention
In order to solve problems of the prior art, it is an object of the invention to provide and a kind of improve animal and deliver speed for sale
Method.
In order to realize the object of the invention, the invention provides α-ketoglutaric acid in terms of improving animal feed intake and body weight
Application, even if animal absorbs α-ketoglutaric acid by oral way.
As preferably, the daily dose of animal consumption α-ketoglutaric acid is 0.05~1.58g/kg with the weight of animals ratio.At this
Under the picked-up daily dose of α-ketoglutaric acid, animal can improve feed intake and body weight significantly.
More preferably, the daily dose of animal consumption α-ketoglutaric acid and the weight of animals are than for 0.30g/kg.In this α-one penta
Under the picked-up daily dose of diacid, improve the best results of animal feed intake and body weight.
The present invention, by many animals carries out absorbing the research of α-ketoglutaric acid, preferably goes out feed intake and substantially carries with body weight
High animal is: mice, rat, pig, fox, rabbit and mink.Above-mentioned animal picked-up α-ketoglutaric acid after, other animals relatively
Improve feed intake and body weight with becoming apparent from.
Wherein, showing more significant animal is ICR mice.The present invention in a specific embodiment, as a example by ICR mice,
Prove beneficial effects of the present invention.
More specifically, for ICR mice, during its 2 monthly age to 10 monthly ages, every day by oral way picked-up α-
Ketoglutaric acid.Described α-ketoglutaric acid by addition in animal feed or drinking water by animal consumption, every day is to described feedstuff
Or drinking water is replaced.
The beneficial effects of the present invention is:
Present invention firstly discovers that, its feed intake and body weight can be improved significantly to detoxification α-ketoglutaric acid, such as
By adding α-ketoglutaric acid in the feedstuff of mice or drinking-water, significantly increase its feed intake and body weight.This mode is not
It is only capable of and accomplishes the most on-demand interpolation, change dosage in time, and be capable of on a large scale under mechanical feed feeds water system
Use.
A-ketoglutaric acid used in the present invention can the most conveniently buied, be applied to improve animal feed intake and
Body weight, it is possible to bring considerable economic benefit and good social benefit, improves plant and the income of cultivation self-employed entrepreneurs, promotes
The development of aquaculture.
Accompanying drawing explanation
Fig. 1 is the impact feeding various dose α-ketoglutaric acid to mice different time feed intake.
Fig. 2 is the impact feeding various dose α-ketoglutaric acid to mice different time body weight.
Detailed description of the invention
Below in conjunction with embodiment, the preferred embodiment of the present invention is described in detail.It will be appreciated that following reality
Execute providing merely to play descriptive purpose of example, be not used to the scope of the present invention is limited.The skill of this area
Art personnel, in the case of without departing substantially from spirit of the invention and spirit, can carry out various amendment and replacement to the present invention.
Experimental technique used in following embodiment if no special instructions, is conventional method.
The reagent and the material that use in following example are commercial goods.Use α-ketoglutaric acid (α-
Ketoglutaric acid, α-KG) purchased from Sigma company (St.Louis, MO, USA).
Embodiment 1
The present embodiment relates to a kind of method improving mammal feed intake and body weight by oral α-ketoglutaric acid, tool
Body is α-ketoglutaric acid to be added in feedstuff or the drinking-water of mice, and it is 0.05g/ that mice takes the daily dose of α-ketoglutaric acid
Kg, daily 1 time.Monthly gather average feed intake and the weight data of mice.
Embodiment 2~5
Compared with Example 1, distinctive points is only that: in embodiment 2~5, and the daily dose taking α-ketoglutaric acid is respectively
0.00g/kg、0.30g/kg、0.78g/kg、1.58g/kg。
Record display, takes when the daily dose of α-ketoglutaric acid is 0.05g/kg, 0.30g/kg and 0.78g/kg and can improve
The average feed intake of mice and average weight.Daily dose 1.58g/kg, lowers mice feed intake and body weight simultaneously.
Experimental example
1. laboratory animal
Using the Kunming white dams about 250 8 week old to carry out above-described embodiment 1~5, Kunming white dams is purchased from army of China
Thing Academy of Medical Sciences Experimental Animal Center, is randomly divided into 5 groups by Kunming white dams, often group 50, and dams can free choice feeding (military affairs
Academy of Medical Sciences Experimental Animal Center produces full nutrition pellet) and drinking-water.
2. experiment condition is: illumination 12h/d, and indoor temperature is 20~22 DEG C.
3. experimentation
By experiment dams after the adaptability raising of a week, in feedstuff or drinking-water, add α-ketoglutaric acid, make dams
The daily dose taking α-ketoglutaric acid is 0.05g/kg to 1.58g/kg, changes feedstuff or drinking-water every day.Monthly record statistics is each
The feed intake of group mice and body weight.
4. experimental result
The data result of above-mentioned record is carried out data process: use spass18.0 statistical software respectively data to be carried out
One factor analysis of variance (ANOVA), P < 0.05 think data have significant difference, p < 0.01 think data difference heteropole show
Write.The multiple comparisons of average uses DuncanShi to carry out, and result Means ± SEM represents.
The impact on mice feed intake of the 4.1 various dose α-ketoglutaric acid
Table 1 feeds various dose α-ketoglutaric acid 1~the impact on mice feed intake in 2 months
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 1, feed middle dosage α-ketoglutaric acid 1~the feed intake of mice within 2 months, can be significantly improved, comparing
6% is improve in matched group.
Table 2 feeds various dose α-ketoglutaric acid 3~the impact on mice feed intake in 4 months
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 2, feed middle dosage α-ketoglutaric acid 2~3 months and can dramatically increase the feed intake of mice, phase in pole
8% is improve compared with matched group.Feed high dose α-ketoglutaric acid 2~the feed intake of mice within 3 months, can also be dramatically increased, its
He does not then have effect at group.
Table 3 feeds various dose α-ketoglutaric acid 5~the impact on mice feed intake in 6 months
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 2, feed low dosage, middle dosage and high dose α-ketoglutaric acid 3~can dramatically increase little in 4 months
The feed intake of Mus, improves 6% compared to matched group.
Table 4 feeds the impact on 7~8 monthly age mice feed intakes of the various dose α-ketoglutaric acid
Note: the different letter of shoulder mark represents difference significant P < 0.05, and shoulder mark is containing same word note: it is poor that the different letter of shoulder mark represents
Different significant P<0.05, shoulder mark represents difference the most significant P>0.05. containing same letter
From upper table 4, along with the prolongation of feeding time, each group feed intake difference disappears.
Comprehensive above table 1, table 2, table 3, table 4 understand, and feed middle dosage α-ketoglutaric acid and can dramatically increase mice teenager
To becoming the feed intake of annual growth stages, promote its growth promoter.
The impact on Mouse Weight of the 4.2 various dose α-ketoglutaric acid
The 2 monthly age CD1 experiment mice weight datas that table 5 has just been bought
Group | α-ketoglutaric acid daily dose | Mice quantity (only) | Average weight (g/ is only) |
Matched group | 0.00g/kg | 50 | 26.97±1.234 |
Low dose group | 0.05g/kg | 50 | 27.30±0.8025 |
Middle dosage group | 0.30g/kg | 50 | 26.51±1.306 |
High dose group | 0.78g/kg | 50 | 27.05±0.7762 |
Maximum dose level group | 1.58g/kg | 50 | 26.57±1.349 |
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 5, also do not carry out 2 monthly age Mouse Weight no significant differences of the random packet of administering transgenic.
Table 6 feeds the impact on Mouse Weight in 1 month of various dose α-ketoglutaric acid continuously
Group | α-ketoglutaric acid daily dose | Mice quantity (only) | Average weight (g/ is only) |
Matched group | 0.00g/kg | 50 | 30.76±1.689a |
Low dose group | 0.05g/kg | 50 | 30.87±1.572a |
Middle dosage group | 0.30g/kg | 50 | 33.12±1.938c |
High dose group | 0.78g/kg | 50 | 32.67±1.807bc |
Maximum dose level group | 1.58g/kg | 50 | 31.8±2.109ab |
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 6, feed middle dosage and the Mouse Weight increase of 1 month of high dose α-ketoglutaric acid group be extremely notable,
The weightening finish of maximum dose level group is notable, improves 7.6% compared to matched group.
Table 7 feeds the impact on Mouse Weight in 2 months of various dose α-ketoglutaric acid continuously
Group | α-ketoglutaric acid daily dose | Mice quantity (only) | Average weight (g/ is only) |
Matched group | 0.00g/kg | 50 | 34±2.069a |
Low dose group | 0.05g/kg | 50 | 34.77±1.975ab |
Middle dosage group | 0.30g/kg | 50 | 35.49±1.613bc |
High dose group | 0.78g/kg | 50 | 35.25±2.071b |
Maximum dose level group | 1.58g/kg | 50 | 34.94±2.15ab |
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 7, compared to matched group, all Mouse Weight increases feeding α-ketoglutaric acid all present notable water
Flat, wherein the middle dosage α-ketoglutaric acid group Mouse Weight increase of 2 months is extremely notable, improves 4.4% compared to matched group.
Table 8 feeds the impact on Mouse Weight in 3 months of various dose α-ketoglutaric acid continuously
Group | α-ketoglutaric acid daily dose | Mice quantity (only) | Average weight (g/ is only) |
Matched group | 0.00g/kg | 50 | 34.81±2.12ab |
Low dose group | 0.05g/kg | 50 | 34.95±2.633ab |
Middle dosage group | 0.30g/kg | 50 | 36.39±2.584c |
High dose group | 0.78g/kg | 50 | 34.6±2.417a |
Maximum dose level group | 1.58g/kg | 50 | 34.63±2.21a |
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 8, feed the middle dosage α-ketoglutaric acid group Mouse Weight increase of 3 months extremely notable, compared to right
4.5% is improve according to group.
Table 9 feeds the impact on Mouse Weight in 4 months of various dose α-ketoglutaric acid continuously
Group | α-ketoglutaric acid daily dose | Mice quantity (only) | Average weight (g/ is only) |
Matched group | 0.00g/kg | 50 | 36.96±2.692b |
Low dose group | 0.05g/kg | 50 | 36.25±2.749bc |
Middle dosage group | 0.30g/kg | 50 | 37.3±2.778c |
High dose group | 0.78g/kg | 50 | 36.62±3.243a |
Maximum dose level group | 1.58g/kg | 50 | 35.13±1.754a |
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 9, compared to matched group, feeding the middle dosage α-ketoglutaric acid group Mouse Weight of 4 months increases pole
Significantly, improve 0.9% compared to matched group.
Table 10 feeds the impact on Mouse Weight in 5 months of various dose α-ketoglutaric acid continuously
Group | α-ketoglutaric acid daily dose | Mice quantity (only) | Average weight (g/ is only) |
Matched group | 0.00g/kg | 50 | 38.98±3.092b |
Low dose group | 0.05g/kg | 50 | 39.76±3.87bc |
Middle dosage group | 0.30g/kg | 50 | 40.92±2.885c |
High dose group | 0.78g/kg | 50 | 37.44±3.256a |
Maximum dose level group | 1.58g/kg | 50 | 38.39±2.98ab |
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 10, compared to matched group, feeding the middle dosage α-ketoglutaric acid group Mouse Weight of 5 months increases pole
Significantly, improve 5% compared to matched group.
Table 11 feeds the impact on Mouse Weight in 6 months of various dose α-ketoglutaric acid continuously
Group | α-ketoglutaric acid daily dose | Mice quantity (only) | Average weight (g/ is only) |
Matched group | 0.00g/kg | 50 | 38.53±3.206b |
Low dose group | 0.05g/kg | 50 | 39.43±3.685bc |
Middle dosage group | 0.30g/kg | 50 | 40.79±3.184c |
High dose group | 0.78g/kg | 50 | 35.94±2.879a |
Maximum dose level group | 1.58g/kg | 50 | 36.94±3.446a |
Note: the different letter of shoulder mark represents difference significant P<0.05, shoulder mark represents the most significant P of difference containing same letter>
0.05.
From upper table 11, compared to matched group, feed low dosage and the middle dosage α-ketoglutaric acid Mouse Weight of 6 months
Increase extremely notable, improve 5.9% compared to matched group.
In summary, feed the α-ketoglutaric acid of various dose to the mice at 2 monthly ages, middle dosage group mice teenager to becoming
Annual growth stages feed intake in early days relatively other groups want height, disappear to later stage feed intake difference, and meanwhile, weight data shows, in
The body weight increase of dosage group mice is always notable or pole is significantly higher than other each group.Middle dosage group mice shifts to an earlier date one than matched group
Within individual month, arrive and specify body weight.Therefore, we guess, middle dosage α-ketoglutaric acid can promote that animal teenager is to the adult stage of development
Feed intake so that its speed of weight increment is accelerated, reach to deliver for sale weight earlier, shorten feeding time and management cost.By 2014
The whole nation can numerous sow breeding stock 496,000,000, every sow annual farrows 1.5 nests, about 10, every nest.Deliver for sale in advance by every nest
Time calculated over 1 month, and average every sow year increases production 1.36, and the annual live pig amount of delivering for sale in the whole nation about improves 676,360,000, directly
Economic benefit about 338,100,000,000 yuan, overall economic benefit is considerable.
Therefore, the method for the present invention can apply in actual production, improves the production performance of animal, reduces animal cultivation
Time and management cost.
Although, the present invention is described in detail the most with a general description of the specific embodiments, but
On the basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (7)
1. α-ketoglutaric acid application in terms of improving animal feed intake and body weight, it is characterised in that make animal by oral side
Formula picked-up α-ketoglutaric acid.
Application the most according to claim 1, it is characterised in that the daily dose of animal consumption α-ketoglutaric acid and the weight of animals
Ratio is 0.05~1.58g/kg.
Application the most according to claim 2, it is characterised in that the daily dose of animal consumption α-ketoglutaric acid and the weight of animals
Ratio is 0.30g/kg.
4. according to the application described in any one of claims 1 to 3, it is characterised in that described animal is mice, rat, pig, Vulpes
Leopard cat, rabbit or mink.
Application the most according to claim 4, it is characterised in that described animal is ICR mice.
Application the most according to claim 5, it is characterised in that absorbed to 10 monthly ages, every day by oral way at 2 monthly ages
α-ketoglutaric acid.
7. according to the application described in claims 1 to 3 or 5~6 any one, it is characterised in that described α-ketoglutaric acid is by adding
It is added in animal feed or drinking water by animal consumption.
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CN201610535051.3A CN106173264A (en) | 2016-07-07 | 2016-07-07 | Alpha Ketoglutarate application in terms of improving animal feed intake and body weight |
CN202010337153.0A CN111387349A (en) | 2016-07-07 | 2016-07-07 | Animal feeding additive and animal feeding method |
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CN105707482A (en) * | 2016-04-14 | 2016-06-29 | 中国科学院亚热带农业生态研究所 | Antibiotic-free cattle feed and preparation method thereof |
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WO1991019489A1 (en) * | 1990-06-08 | 1991-12-26 | The Upjohn Company | METHOD FOR REGULATING RUMINAL pH |
CN102028102A (en) * | 2010-12-08 | 2011-04-27 | 江南大学 | Natural and safe growth-type feed additive |
CN103340293B (en) * | 2013-07-22 | 2014-05-07 | 湖南农业大学 | Use of alpha-ketoglutaric acid |
CN105029086A (en) * | 2015-08-26 | 2015-11-11 | 中国科学院亚热带农业生态研究所 | Application of alpha-oxoglutarate disodium in preparation of pig feed |
CN105325731A (en) * | 2015-12-07 | 2016-02-17 | 湖南农业大学 | Application of alpha-ketoglutarate serving as feed additive and corresponding feed |
CN105475230B (en) * | 2015-12-17 | 2018-04-20 | 中国农业大学 | A kind of method for improving above the average age for marriage animal reproduction power |
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- 2016-07-07 CN CN202010293512.7A patent/CN111374222A/en active Pending
- 2016-07-07 CN CN201610535051.3A patent/CN106173264A/en active Pending
- 2016-07-07 CN CN202010337153.0A patent/CN111387349A/en active Pending
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003055508A1 (en) * | 2001-12-21 | 2003-07-10 | Essentys Ab | Composition for the treatment of renal failure or gastric dysfunction of e.g. premature or neonatal mammals |
CN105231038A (en) * | 2015-09-23 | 2016-01-13 | 华南农业大学 | Application of alpha-oxoglutarate in respect of preparing pig feed additive |
CN105707482A (en) * | 2016-04-14 | 2016-06-29 | 中国科学院亚热带农业生态研究所 | Antibiotic-free cattle feed and preparation method thereof |
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