CN106172555A - A kind of compositions preventing tobacco bacterial wilt - Google Patents
A kind of compositions preventing tobacco bacterial wilt Download PDFInfo
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- CN106172555A CN106172555A CN201610551308.4A CN201610551308A CN106172555A CN 106172555 A CN106172555 A CN 106172555A CN 201610551308 A CN201610551308 A CN 201610551308A CN 106172555 A CN106172555 A CN 106172555A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
Abstract
The invention discloses the preparation method of a kind of compositions preventing tobacco bacterial wilt: the preparation of (1) Rhizoma Zingiberis Recens extract；(2) preparation of soapberry extract；(3) preparation of Aralia mandshrica extract；(4) preparation of leaf of Vaccinium bracteatum Thunb. extract；(5) according to parts by weight, take Rhizoma Zingiberis Recens extract 8 12 parts, soapberry extract 35 parts, Aralia mandshrica 23 parts, leaf of Vaccinium bracteatum Thunb. extract 24 parts, wood vinegar 0.5 0.9 parts, pure water 100 parts, be mixed to get the plant extract of compositions for preventing tobacco bacterial wilt.Result: induction of resistance active effect is 70.2 92.3%.Later of the pinching moon, relative to matched group sickness rate 53.5%, the flue-cured tobacco sickness rate that the present embodiment processes is only 6.3 10.8%, and prevention effect is 51.2 69.8%.
Description
Technical field
The invention belongs to the present invention relates to plant pest green ecological Prevention Technique field, particularly a kind of prevention Nicotiana tabacum L.
The compositions of bacterial wilt.
Background technology
Tobacco bacterial wilt, also known as cigarette pestilence, half of crazy, Granville wilt disease (Granvillewilt), is by Ralstonia
The bacillary soil-borne disease that solanacearum causes.Now this disease is distributed widely in the torrid zone, subtropical zone and ground that some are warm
District, is the common important disease in south China tobacco planting district.Fall ill particularly in Guangdong, Hunan, Fujian, Guizhou and Deng Yan district, Sichuan
Seriously, the biggest loss is brought to leaf tobacco production.Tobacco bacterial wilt can be rated as Nicotiana tabacum L. " cancer ", there is no special effect agent preventing and treating at present,
And Biological control has safety nuisance free, the advantage such as long-acting, it it is the focus of disease control research.
Ralstonia solanacearum Yabuuhi et al. claims Ralstonia solanacearum, belongs to antibacterial.Thalline is shaft-like,
The blunt circle in two ends, size 0.9 2 × 0.5-0.8 (um), there are 13 flagellums, many one poles are raw, without pod membrane, Gram-negative,
Aerobic.Pathogen growth temperature limits 10 DEG C 37 DEG C, and 30 35 DEG C the suitableeest.52 DEG C lethal through 10 minutes.Optimum pH 6.6.This bacterium is
Identifying 5 microspecies and 5 biotypes, the bacterial strain infecting Nicotiana tabacum L. is microspecies 1 and biotype I, III, IV, under natural conditions, biological
Type the most often changes.Pathogenic bacteria mainly in soil, be lost on the invalid body in soil and in the pin main body that grows and rhizosphere more
Winter.Seed does not carries disease germs.Main primary infection inoculum is the pathogen in soil, invalid tissue and fertilizer.Pathogen borrows irrigation and drainage
Water, flowing water, the fertilizer that carries disease germs, sick Seedling or the sick soil being attached on seedling, and people and animals and the tool of production carry disease germs propagation.Sick field flowing water is
The most important mode that disease infects and propagates.
The principal element that bacterial wilt occurs includes: soil, weather, fertilizer are waterlogging-resistant with measures of fertilizer, water project situation and drought resisting
The environmental conditions such as measure；Vega cause of disease cumulant；The disease resistance of tobacco bred；Cigarette strain growth and development state and well developed root system journey
Degree；Tobacco field fertilizing level and fertilizer type；Disease finds and the opportunity of preventing and treating.
Above-mentioned factor influences each other, and interacts.As a whole, cause of disease radix is big, soil property is bad and variety resistance is poor
Being to cause bacterial wilt large area that sick basic reason occurs, climate change is the odjective cause causing plant disease epidemic, artificially payes attention to
Not and the technical measures subjective reason being to cause plant disease epidemic not in place.Soil infection, early stage are infected, concentrate after rain the morbidity to be
The typical way that bacterial wilt occurs.There is the dead cigarette of large area in You Yan district, it is simply that due to infecting of early stage bacterial wilt, after adding rain
Transpiration cause.
The root system development situation of Nicotiana tabacum L., improving activity of root system are closely related with its disease resistance, and the appropriate arid after tobacco seedlings is transplanted has
Be beneficial to the growth of tobacco root, if tobacco seedlings transplant after vega high humidity or water again in 4 weeks, especially to vega after transplanting
Interior Nitrogen Top Dressing, cigarette strain root system development can be caused to be obstructed, vigor poor, add transpiration, be easily caused disease large area occur.
The basis of chemical prevention depends on the growth basis of Nicotiana tabacum L., and this is also that some prevents and treats poor former of the effect of chemical control of bacterial wilt
One of because of.
It is desirable to provide a kind of compositions preventing tobacco bacterial wilt, this product compared with prior art, sky of drawing materials
So, tobacco bacterial wilt can effectively be prevented.
Summary of the invention
It is an object of the invention to provide a kind of compositions preventing tobacco bacterial wilt, this product compared with prior art, takes
Material is natural, can effectively prevent tobacco bacterial wilt.
In order to realize above-mentioned purpose, present invention employs following technical scheme:
The preparation method of a kind of compositions preventing tobacco bacterial wilt, it is characterised in that step is as follows:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of Aralia mandshrica extract, dries Aralia mandshrica, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of leaf of Vaccinium bracteatum Thunb. extract, dries leaf of Vaccinium bracteatum Thunb., pulverizes, and presses 1:3 with methanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, Rhizoma Zingiberis Recens extract 8-12 part, soapberry extract 3-5 part, Aralia mandshrica 2-3 part, leaf of Vaccinium bracteatum Thunb. are taken
Extract 2-4 part, wood vinegar 0.5-0.9 part, pure water 100 parts, it is mixed to get for preventing planting of the compositions of tobacco bacterial wilt
Thing extract.
The using method of above-mentioned composition is: respectively in tobacco transplant, fragmentary morbidity, prosperous for a long time medicament is uniformly sprayed at
The page, 20 mL/ strain sprinklings process, coprocessing 3 times.
For examination flue-cured tobacco cultivars cloud and mist 97 kind, conventional flue-cured tobacco cultivars, Yun yan85 combine selection-breeding with CV 87 and form.
The investigation of cigarette strain incidence uses the mode of community generaI investigation, by GB/23222-2008 " tobacco disease insect pest classification and tune
Checking method " investigate and manage incidence everywhere, and calculate each process disease index, prevention effect.Matched group uses clear water to process.
Tobacco bacterial wilt severity Scaling standard (in units of strain):
0 grade: Herb is anosis；
1 grade: stem is even chlorisis speckle, or sick side 1/2 is wilting with lower blade；
3 grades: there is black streak in stem, but less than 1/2, or sick side 1/2～2/3 blade is wilting；
5 grades: stem's black streak is more than 1/2, but does not arrives stem top, or sick side 2/3 is wilting with blade；
7 grades: stem's black streak arrives stem top, or diseased plant blade is the most wilting；
Sickness rate (%)=diseased plant number/investigation strain number × 100
Disease index=Σ (morbidity strain number × this disease level representative value)/(investigating total strain number × superlative degree representative value) × 100
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100
The invention have benefit that:
1. invention aims to provide a kind of compositions preventing tobacco bacterial wilt, and this product compared with prior art, takes
Material is natural, can effectively prevent tobacco bacterial wilt.
2. consumption is few, cost-effective.
Detailed description of the invention
Embodiment 1:
The preparation method of a kind of compositions preventing tobacco bacterial wilt, it is characterised in that step is as follows:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of Aralia mandshrica extract, dries Aralia mandshrica, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of leaf of Vaccinium bracteatum Thunb. extract, dries leaf of Vaccinium bracteatum Thunb., pulverizes, and presses 1:3 with methanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, Rhizoma Zingiberis Recens extract 8 parts, soapberry extract 5 parts, Aralia mandshrica 2 parts, leaf of Vaccinium bracteatum Thunb. extract 4 are taken
Part, wood vinegar 0.5 part, pure water 100 parts, it is mixed to get the plant extract of compositions for preventing tobacco bacterial wilt.
The using method of above-mentioned composition is: respectively in tobacco transplant, fragmentary morbidity, prosperous for a long time medicament is uniformly sprayed at
The page, 20 mL/ strain sprinklings process, coprocessing 3 times.
For examination flue-cured tobacco cultivars cloud and mist 97 kind, conventional flue-cured tobacco cultivars, Yun yan85 combine selection-breeding with CV 87 and form.
Result: later of the pinching moon, relative to matched group sickness rate 53.5%, the flue-cured tobacco sickness rate that the present embodiment processes is only
Being 10.8%, prevention effect is 51.2%.
Embodiment 2:
The preparation method of a kind of compositions preventing tobacco bacterial wilt, it is characterised in that step is as follows:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of Aralia mandshrica extract, dries Aralia mandshrica, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of leaf of Vaccinium bracteatum Thunb. extract, dries leaf of Vaccinium bracteatum Thunb., pulverizes, and presses 1:3 with methanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, Rhizoma Zingiberis Recens extract 12 parts, soapberry extract 3 parts, Aralia mandshrica 3 parts, leaf of Vaccinium bracteatum Thunb. extract are taken
2 parts, wood vinegar 0.9 part, pure water 100 parts, it is mixed to get the plant extract of compositions for preventing tobacco bacterial wilt.
The using method of above-mentioned composition is: respectively in tobacco transplant, fragmentary morbidity, prosperous for a long time medicament is uniformly sprayed at
The page, 20 mL/ strain sprinklings process, coprocessing 3 times.
For examination flue-cured tobacco cultivars cloud and mist 97 kind, conventional flue-cured tobacco cultivars, Yun yan85 combine selection-breeding with CV 87 and form.
Result: later of the pinching moon, relative to matched group sickness rate 53.5%, the flue-cured tobacco sickness rate that the present embodiment processes is only
Being 6.3%, prevention effect is 69.8%.
Embodiment 3:
The preparation method of a kind of compositions preventing tobacco bacterial wilt, it is characterised in that step is as follows:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of Aralia mandshrica extract, dries Aralia mandshrica, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of leaf of Vaccinium bracteatum Thunb. extract, dries leaf of Vaccinium bracteatum Thunb., pulverizes, and presses 1:3 with methanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, Rhizoma Zingiberis Recens extract 9 parts, soapberry extract 4 parts, Aralia mandshrica 2 parts, leaf of Vaccinium bracteatum Thunb. extract 3 are taken
Part, wood vinegar 0.6 part, pure water 100 parts, it is mixed to get the plant extract of compositions for preventing tobacco bacterial wilt.
The using method of above-mentioned composition is: respectively in tobacco transplant, fragmentary morbidity, prosperous for a long time medicament is uniformly sprayed at
The page, 20 mL/ strain sprinklings process, coprocessing 3 times.
For examination flue-cured tobacco cultivars cloud and mist 97 kind, conventional flue-cured tobacco cultivars, Yun yan85 combine selection-breeding with CV 87 and form.
Result: later of the pinching moon, relative to matched group sickness rate 53.5%, the flue-cured tobacco sickness rate that the present embodiment processes is only
Being 9.4%, prevention effect is 54.7%.
Embodiment 4:
The preparation method of a kind of compositions preventing tobacco bacterial wilt, it is characterised in that step is as follows:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of Aralia mandshrica extract, dries Aralia mandshrica, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of leaf of Vaccinium bracteatum Thunb. extract, dries leaf of Vaccinium bracteatum Thunb., pulverizes, and presses 1:3 with methanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, Rhizoma Zingiberis Recens extract 11 parts, soapberry extract 5 parts, Aralia mandshrica 3 parts, leaf of Vaccinium bracteatum Thunb. extract are taken
4 parts, wood vinegar 0.8 part, pure water 100 parts, it is mixed to get the plant extract of compositions for preventing tobacco bacterial wilt.
The using method of above-mentioned composition is: respectively in tobacco transplant, fragmentary morbidity, prosperous for a long time medicament is uniformly sprayed at
The page, 20 mL/ strain sprinklings process, coprocessing 3 times.
For examination flue-cured tobacco cultivars cloud and mist 97 kind, conventional flue-cured tobacco cultivars, Yun yan85 combine selection-breeding with CV 87 and form.
Result: later of the pinching moon, relative to matched group sickness rate 53.5%, the flue-cured tobacco sickness rate that the present embodiment processes is only
Being 7.7%, prevention effect is 67.4%.
Embodiment 5:
The preparation method of a kind of compositions preventing tobacco bacterial wilt, it is characterised in that step is as follows:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of Aralia mandshrica extract, dries Aralia mandshrica, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of leaf of Vaccinium bracteatum Thunb. extract, dries leaf of Vaccinium bracteatum Thunb., pulverizes, and presses 1:3 with methanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) according to parts by weight, Rhizoma Zingiberis Recens extract 10 parts, soapberry extract 3 parts, Aralia mandshrica 2 parts, leaf of Vaccinium bracteatum Thunb. extract are taken
2 parts, wood vinegar 0.7 part, pure water 100 parts, it is mixed to get the plant extract of compositions for preventing tobacco bacterial wilt.
The using method of above-mentioned composition is: respectively in tobacco transplant, fragmentary morbidity, prosperous for a long time medicament is uniformly sprayed at
The page, 20 mL/ strain sprinklings process, coprocessing 3 times.
For examination flue-cured tobacco cultivars cloud and mist 97 kind, conventional flue-cured tobacco cultivars, Yun yan85 combine selection-breeding with CV 87 and form.
Result: later of the pinching moon, relative to matched group sickness rate 53.5%, the flue-cured tobacco sickness rate that the present embodiment processes is only
Being 8.1%, prevention effect is 60.1%.
Visible, the present invention provides a kind of compositions preventing tobacco bacterial wilt, and this product compared with prior art, takes
Material is natural, can effectively prevent tobacco bacterial wilt.Consumption is few, cost-effective.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope that the invention discloses, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (3)
1. the preparation method of the compositions preventing tobacco bacterial wilt, it is characterised in that step is as follows:
(1) preparation of Rhizoma Zingiberis Recens extract, dries Rhizoma Zingiberis Recens, pulverize, with methanol press 1:5 extract twice, filtration, filtrate 30 DEG C with
Lower Rotary Evaporators concentrating under reduced pressure, obtains extractum, and cryopreservation is standby；
(2) preparation of soapberry extract, dries Fructus Sapindi Mukouossi, pulverizes, and presses 1:5 with water and extracts three times, filters, and filtrate is at 30 DEG C
Hereinafter using Rotary Evaporators concentrating under reduced pressure, obtain extractum, cryopreservation is standby；
(3) preparation of Aralia mandshrica extract, dries Aralia mandshrica, pulverizes, and presses 1:3 with ethanol and extracts three times, filters, and filtrate is 30
Using Rotary Evaporators concentrating under reduced pressure below DEG C, obtain extractum, cryopreservation is standby；
(4) preparation of leaf of Vaccinium bracteatum Thunb. extract, dries leaf of Vaccinium bracteatum Thunb., pulverizes, and presses 1:3 with methanol and extracts three times, filters, filter
Liquid uses Rotary Evaporators concentrating under reduced pressure below 30 DEG C, obtains extractum, and cryopreservation is standby；
(5) Rhizoma Zingiberis Recens extract is taken, soapberry extract, Aralia mandshrica, leaf of Vaccinium bracteatum Thunb. extract, wood vinegar, pure water, it is mixed to get use
Plant extract in the compositions of prevention tobacco bacterial wilt.
2. the preparation method of the compositions of the prevention tobacco bacterial wilt described in claim 1, it is characterised in that:
Described step (5) is: according to parts by weight, takes Rhizoma Zingiberis Recens extract 8-12 part, soapberry extract 3-5 part, Aralia mandshrica 2-3
Part, leaf of Vaccinium bracteatum Thunb. extract 2-4 part, wood vinegar 0.5-0.9 part, pure water 100 parts, it is mixed to get for preventing tobacco bacterial wilt
The plant extract of compositions.
3. the compositions of the prevention tobacco bacterial wilt that the method described in claim 1-2 prepares.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107413160A (en) * | 2017-04-27 | 2017-12-01 | 舒尔环保科技（合肥）有限公司 | A kind of material for air purification and preparation method thereof |
| CN107779420A (en) * | 2017-06-27 | 2018-03-09 | 湖北省烟草公司恩施州公司 | A kind of Nei Shengbeilaisi bacillus of two plants of antagonism tobacco bacterial wilts and its application |
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| CN105145691A (en) * | 2015-09-18 | 2015-12-16 | 张滋柠 | Household preparation and use method of biological insecticidal bacteriostatic agent |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107413160A (en) * | 2017-04-27 | 2017-12-01 | 舒尔环保科技（合肥）有限公司 | A kind of material for air purification and preparation method thereof |
| CN107779420A (en) * | 2017-06-27 | 2018-03-09 | 湖北省烟草公司恩施州公司 | A kind of Nei Shengbeilaisi bacillus of two plants of antagonism tobacco bacterial wilts and its application |
| CN107779420B (en) * | 2017-06-27 | 2020-10-30 | 湖北省烟草公司恩施州公司 | Two-strain endogenous Bacillus belgii for antagonizing tobacco bacterial wilt and application thereof |
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