CN106172404A - A kind of containing SYP-3375 and the bactericidal composition of bromothalonil and application - Google Patents
A kind of containing SYP-3375 and the bactericidal composition of bromothalonil and application Download PDFInfo
- Publication number
- CN106172404A CN106172404A CN201610595215.1A CN201610595215A CN106172404A CN 106172404 A CN106172404 A CN 106172404A CN 201610595215 A CN201610595215 A CN 201610595215A CN 106172404 A CN106172404 A CN 106172404A
- Authority
- CN
- China
- Prior art keywords
- syp
- bromothalonil
- bactericidal composition
- preventing
- treating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/34—Nitriles
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Toxicology (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of containing SYP-3375 with the bactericidal composition of bromothalonil, effective ingredient is SYP-3375 and bromothalonil, and SYP-3375 is 5:1～1:35 with the weight ratio of bromothalonil.Can also be containing Pesticide Science allowing use and acceptable adjuvant, dosage form can be wettable powder, suspending agent, cream, aqueous emulsion, water dispersible granules or microemulsion.The bactericidal composition of the present invention, the mechanism of action of effective ingredient SYP-3375 and bromothalonil is entirely different, compounds and can act in sites many to pathogen, Synergistic, expands fungicidal spectrum, improves preventive effect, reduce Pesticide use amount, delay drug-fast generation, reduce resistance risk.The bactericidal composition of the present invention, for preventing and treating the multiple trunk disease on fruit tree, forest, is simultaneously available for preventing and treating various vegetables and the fruit diseases such as spot defoliation, scab, anthrax.It is particularly suited for the diseases such as canker of apple fruit, eyespot in apple limb, Fructus Pruni pseudocerasi bleeding disease, Ramulus Juglandis rot.
Description
Technical field
The present invention relates to a kind of containing SYP-3375 with the bactericidal composition of bromothalonil, and preventing and treating fruit tree, forest branch
Disease and vegetable disease are applied, belongs to the application of pesticide control technical field.
Background technology
Trunk disease is one of Major Diseases of fruit tree, forest, and its incidence rate, hazardness increase the weight of year by year in recent years, becomes
For harm fruit industry, the great disease of production of forestry.Fruit tree, forest trunk disease include canker, Trunk canker, bleeding disease,
The diseases such as dry rot, Peptic Ulcers, branch-rot, wherein, apple tree Trunk canker popularizing along with fruit bagging technology in recent years
Promoting generation increasingly severe, fall ill the heaviest with Fuji, red magnitude seeds, diseased plant rate reaches more than 90%；Apple tree canker
The major branch of main harm apple tree and side shoot, cause Fructus Mali pumilae tree vigo(u)r weak, and fruit yield and quality decline, serious orchard of falling ill
A large amount of dead trees even ruin garden；Widely, and its parasitics is weak, adds SPS for the host range of dothiorella gregaria pathogenic bacteria
The most capable, cause a large amount of pathogenic bacteria to be spread along with the allocation and transportation of nursery stock, cause dothiorella gregaria occur increasingly severe, particularly
Bigger to the nursery stock at the initial stage of planting and the harm of treelet, cause the death of a large amount of nursery stock, cause the failure of whole spring afforestation, according to
Report, in the area that morbidity is serious, the sickness rate of forest is up to 96.1%～100%, and dothiorella gregaria has become development China
The serious hindrance of Poplar Plantation.
In view of the order of severity of trunk disease harm, recent domestic scholar has carried out substantial amounts of research to it, and has carried
Go out many effectively preventing measures, but in today of wildness outburst, every prophylactico-therapeutic measures all difficulties have reached preferable prevention effect.
The preferable medicament asomate of the most conventional preventing and treating trunk disease belongs to high poison, persistent pesticide, and the Ministry of Agriculture is complete the end of the year 2015
Face forbids selling and using, and other medicaments such as lime sulfur, copper humate etc., although play a role, but exist relatively
Many problems and shortcomings, are mainly manifested in model of action single, and prevention effect is unstable, the aspects such as the drug effect phase is short, cause overall anti-
Controlling effect undesirable, scab relapse rate is high, and wound healing is poor.Therefore, low toxicity a kind of efficiently in the urgent need to invention, environmental protection, can
Thorough sterilization can promote again preventing and treating fruit tree, the therapeutic agent of forest trunk disease of the low cost of wound healing.
Methoxy acrylic acid series bactericidal agent is antibacterial kind the most newly developed, is a big mileage in antibacterial field
Upright stone tablet.Methoxy acrylic bactericide has the mechanism of action of uniqueness, and it is by pinning the electronics between cytochrome b and c1
Transmit and stop the ATP of cell to synthesize, thus suppress its mitochondrial respiratory and play bacteriostasis.14-demethylation enzyme is pressed down
The bacterial strain that preparation, benzamides, dicarboxylic dihydrazides amine and benzimidazole produce resistance is effective.Have protection, treat, root out,
Infiltration, systemic activity, and can be in plant, soil and water are quickly degraded.Although this series bactericidal agent mechanism of action is unique,
But pathogen produces the speed of resistance the most quickly to it, use cost is high, so limiting methoxy acrylic acid series bactericidal agent
Spread uses.SYP-3375 is a kind of methoxy-methyl acrylate compounds, English common name:
Coumoxystrobin, chemical name: (E)-2-(2-((3-butyl-4-methyl-coumarin-7-base-epoxide) methyl) benzene
Base)-3-methoxy-methyl acrylate.There is protection and therapeutical effect, be the disinfectant use in agriculture of a kind of high-efficiency broad spectrum, to by flagellum
Bacterium, combine plant disease such as apple tree canker that bacterium, ascomycetes, basidiomycetes and Fungi Imperfecti cause, apple tree ring spot, Oryza sativa L.
Rice blast, sigatoka, alternaria leaf spot of apple etc. have good preventive effect.
Bromothalonil, english common name: bromothalonil, chemical formula: C6H6Br2N2, chemical name 1,2'-bis-is bromo-
2,4-dicyanobutanes, are a kind of wide spectrum, low toxicity, mildew-resistant, algae-killing bactericidal agents.Can suppress and root out antibacterial, fungus and algae
Growth, has preferable prevention effect to corps diseases.It is widely used in fruit tree, Fructus Vitis viniferae, vegetable, Cotton Gossypii, Semen arachidis hypogaeae, Citrullus vulgaris, cigarette
The various crop such as grass, Camellia sinensis, flowers, prevent and treat anthrax, scab, shot hole, powdery mildew, rust, damping-off, damping off, root
The multiple kinds of crops diseases such as stem rot.
At present, there is not yet Flos Caryophylli chrysanthemum ester and bromothalonil are carried out compounding use and be used for preventing and treating fruit tree, forest branch,
The relevant report of vegetable disease.
Summary of the invention
For above-mentioned prior art, the invention provides a kind of containing SYP-3375 with the bactericidal composition of bromothalonil, and
Preventing and treating fruit tree, forest branch, vegetable disease are applied.
The present invention is achieved by the following technical solutions:
A kind of containing SYP-3375 with the bactericidal composition of bromothalonil, effective ingredient is SYP-3375 and bromothalonil, Flos Caryophylli bacterium
Ester is 5:1～1:35 with the weight ratio of bromothalonil, it is preferred that for 1:1～15.
In the described bactericidal composition containing SYP-3375 and bromothalonil, can be containing Pesticide Science allowing use and can connect
The adjuvant being subject to.
Further, the described bactericidal composition containing SYP-3375 and bromothalonil is made up of the component of following weight portion
: Wu Junzuo 1%～80%, octyl trichlamide 1%～80%, surplus is to allow in Pesticide Science to use and acceptable adjuvant.
Described Pesticide Science allows use and be selected from solvent, penetrating agent, dispersant, diffusant with acceptable adjuvant, collapse
Solve agent, emulsifying agent, stabilizer, wetting agent, defoamer, antifreezing agent etc. and other contributes to the stable of effective ingredient drug effect and plays
Allow make used additives.
Described containing SYP-3375 with the bactericidal composition of bromothalonil, can prepare by processing method well known in the prior art
Becoming to be suitable for any one dosage form that agricultural production uses, preferred dosage form is: wettable powder, suspending agent, cream, aqueous emulsion, water
Dispersible granule, microemulsion.
The present invention containing SYP-3375 and the bactericidal composition of bromothalonil, be used for preventing and treating fruit tree, forest (apple tree, Fructus Pruni pseudocerasi
Tree, pear tree, peach tree, jujube tree, Semen Castaneae, Semen Juglandis, Caulis et folium euphorbiae milii, willow, willow etc.) on multiple trunk disease, be simultaneously available for preventing and treating
Various vegetables and the fruit diseases such as spot defoliation, scab, anthrax, be particularly suited for canker of apple fruit, Fructus Mali pumilae branch wheel
The disease such as stricture of vagina disease, Fructus Pruni pseudocerasi bleeding disease, peach gummosis, dothiorella gregaria, Ramulus Persicae rot, alternaria leaf spot of apple, Semen Juglandis anthrax
Evil.Preferably, when preventing and treating Valsa mali, SYP-3375 is preferred with 5:1～1:35 with the weight ratio of both bromothalonils, and 1:
5～25 more preferably, and 1:10 is optimal.When preventing and treating eyespot in apple limb bacterium, the weight ratio of SYP-3375 and both bromothalonils is with 1:2
～15 be preferred, more preferably, 1:10 is optimal for 1:5～15.When preventing and treating Fructus Pruni pseudocerasi gummosis pathogenic bacteria, SYP-3375 and the weight of both bromothalonils
Ratio is preferred with 1:2～15, and more preferably, 1:10 is optimal for 1:5～15.When preventing and treating dothiorella gregaria bacterium, SYP-3375 and bromothalonil two
The weight ratio of person is preferred with 1:2～15, and more preferably, 1:10 is optimal for 1:5～15.Prevent and treat Ramulus Persicae rot bacterium time, SYP-3375 with
The weight ratio of both bromothalonils is preferred with 2:1～1:35, and more preferably, 1:10 is optimal for 1:5～20.At preventing and treating Alternaria mali
Time, SYP-3375 is preferred with 2:1～1:25 with the weight ratio of both bromothalonils, and more preferably, 1:5 is optimal for 1:2～15.At preventing and treating Semen Juglandis
During anthrax bacteria, SYP-3375 is preferred with 1:2～15 with the weight ratio of both bromothalonils, and 1:10 is optimal.It addition, the present invention's contains
The bactericidal composition of SYP-3375 and bromothalonil is alternatively arranged as fruit tree, forest grafting, the sealing compound of beta pruning.
Although SYP-3375 is the antibacterial of a kind of high-efficiency broad spectrum, but as methoxy acrylic ester compounds, effect
Mechanism is single, and field is reused pathogenic bacteria for a long time and is easily generated resistance, causes preventive effect to reduce, and use cost is high.The present invention sends out
A person of good sense is found by experiment that, by bactericide compounded to SYP-3375s different for the mechanism of action and bromothalonil, has the most collaborative increasing
Effect effect, and can improve medicament osmosis, extends the lasting period, not only significantly improve to fruit tree, the treatment of forest trunk disease,
Protected effect, simultaneously by the treatment protection of tree body is added conditioning, reaches the purpose cured trunk disease and prevent it from recurring.
The present invention containing SYP-3375 and the bactericidal composition of bromothalonil, effective ingredient SYP-3375 and the effect of bromothalonil
Mechanism is entirely different, compounds and can act in sites many to pathogen, and Synergistic expands fungicidal spectrum, improves preventive effect, reduces pesticide and makes
Consumption, delays drug-fast generation, reduces resistance risk.
The present invention containing SYP-3375 and the bactericidal composition of bromothalonil, can provide with finished product preparation form, it is also possible to single
Agent form provides, and before using, directly mixing, is then watered mixing and is made into desired concn, smear after striking off branch position disease scar, with
Shi Peihe whole plant spraying using effect is more preferably.
The present invention containing SYP-3375 and the bactericidal composition of bromothalonil, have the advantage that 1, there is Synergistic make
With, improve prevention effect；2, fungicidal spectrum is expanded；3, two kinds of effective ingredient mechanisms of action are different, and mixture uses and delays pathogen to resist
The generation of the property of medicine；4, reduce formulation rate, reduce access times, reduce use cost.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.
Instrument involved in following embodiment, reagent, material etc., unless otherwise noted, be in prior art existing
Conventional instrument, reagent, material etc., can be either commercially available by regular.Experimental technique involved in following embodiment, inspection
Survey methods etc., unless otherwise noted, are existing normal experiment method in prior art, detection method etc..
The preventive and therapeutic effect of disease is studied (Toxicity Determination) by experiment SYP-3375 and bromothalonil compounded
One, reagent agent: the former medicine of SYP-3375, the former medicine of bromothalonil, and thiophanate-methyl, Tebuconazole.96% Flos Caryophylli bacterium
The former medicine of ester first dissolves with a small amount of DMF (DMF), then with the water containing the tween 80 that volume fraction is 0.2%
Solution dilution is made into the mother solution of 10000 μ g/mL, by the 97% former medicinal acetone solution of bromothalonil, and is made into the mother of 10000 μ g/mL
Liquid, the volume fraction with 0.2% adds emulsifying agent Tween-80, preserves standby in refrigerator at 4 DEG C, dilute with sterilized water during use
Release to debita spissitudo.
Two, for examination pathogen: Valsa mali Valsa mali, eyespot in apple limb bacterium Botryosphaeria
Dothidea, Ramulus Persicae rot bacterium Phomopsis amygdali, Fructus Pruni pseudocerasi gummosis pathogenic bacteria Botryosphaeria dothidea, poplar
Tree ulcer bacteria Botryosphaeria dothidea, Alternaria mali Alternaria mali, Semen Juglandis anthrax
Bacterium Colletotrichum gloeosporioides.
Three, assay method:
(1) Toxicity Determination: use mycelial growth rate method that SYP-3375, bromothalonil and mixture preparation thereof are carried out poison
Power measures.
Single dose toxicity test method:
First reagent agent (including SYP-3375, bromothalonil, thiophanate-methyl, ambam, Difenoconazole) is configured to 7
Individual Concentraton gradient (SYP-3375: 1.0,2.0,4.0,8.0,16.0,32.0,7 Concentraton gradient such as 64.0mg/kg；Bromothalonil
1.0,2.0,4.0,8.0,16.0,32.0,7 Concentraton gradient such as 64.0mg/kg；Thiophanate-methyl 0.5,1.0,2.0,4.0,
8.0,16.0,7 Concentraton gradient such as 32.0mg/kg；Ambam 3.0,6.0,12.0,24.0,48.0,96.0,192.0mg/kg
Deng 7 Concentraton gradient；Difenoconazole 1.0,2.0,4.0,8.0,16.0,32.0,7 Concentraton gradient such as 64.0mg/kg), will
Pathogen is 26 DEG C of precultures 4d in PDA culture medium, are beating on the same circumference of colony edge with the card punch of diameter 7mm
Taking bacterium cake, be inoculated on the culture medium flat plate containing different agents concentration, every ware is a piece of, cultivates 4d in being placed in 26 DEG C of calorstats,
Measure the colony growth diameter of each process by decussation method, with clear water for comparison, every concentration processes and is repeated 4 times.Measure bacterium colony
The most linear increment, determines the medicament suppression ratio to colony growth.By mycelial growth suppression probit and drug concentration pair
Linear regression analysis between numerical value, obtains each medicament to concentration (EC in the effectively suppression of bacterial strain50Value).
Mixed agent joint toxicity measuring:
On the basis of single dose toxicity test, its EC will be pressed respectively for the SYP-3375 of mixture, bromothalonil50Value dosage presses body
Long-pending ratio is respectively provided with 10: 1,5: 1,2: 1,1: 1,1: 2,1: 5,1: 10,1: 15,1: 20,1: 25,1: 30,1: 35,1: 40,1:
45,1: 50 totally 15 proportionings, being configured to final mass concentration (referring to Flos Caryophylli chrysanthemum ester, the gross mass of bromothalonil) by different ratio is
0.25,0.5,1.0,2.0,4.0,8.0, the pastille PDA culture medium of 7 series concentration of 16.0mg/kg, determine that medicament is to bacterium colony
The suppression ratio of growth.Often process (the every concentration level of every bacterial strain) to be repeated 4 times.By mycelial growth suppression probit and drug concentration
Linear regression analysis between logarithm value, obtains Mixed Pharmacy to concentration EC in the effectively suppression of bacterial strain50Value and CTC value, with not
The PDA culture medium of adding medicine is as comparison.
(2) data statistic analysis
Test data is carried out statistical analysis by Microsoft Excel 2003, DPS data processing work platform, calculates
Go out the EC of every kind of medicament50Value, 95% confidence limit.The abundant method of Sun Yun (1960) is utilized to calculate the co-toxicity coefficient of mixed agent, according to common poison
Coefficient magnitude evaluates the potentiation of mixed agent.
According to the below equation each medicament suppression ratio of calculating:
Developing restraint rate (%)=[(comparison antibacterial circle diameter-process antibacterial circle diameter)/(comparison antibacterial circle diameter-5mm
Punching diameter)] × 100；
Developing restraint rate (%)=[(comparison colony diameter-process colony diameter)/(comparison colony diameter-bacterium cake diameter)]
×100；
Toxicity index TI=(standard agent EC50/ reagent agent EC50)×100；
Mixed agent actual toxicity index ATI=(standard agent EC50/ mixture preparation EC50)×100；
(PA and PB is respectively mixed agent to the TI × PB of TI × PA+ single dose B of mixed agent theory toxicity index TTI=single dose A
The percentage composition of middle effective ingredient)；
Theoretical toxicity index TTI × 100 of the actual toxicity index ATI/ mixed agent of co-toxicity coefficient CTC=mixed agent；
Potentiation judges: CTC >=120, has potentiation；80 ＜ CTC ＜ 120, for summation action；CTC≤80, for
Antagonism.
(3) result and analysis: as shown in table 1～table 8.
The 15 kinds of medicaments of the table toxicity test result to Valsa mali
Table 2 SYP-3375 and the bromothalonil different proportion mixture toxicity test result to Valsa mali
From table 1～2, SYP-3375, bromothalonil and mixture preparation thereof, Valsa mali is had well suppression
Effect, when wherein the proportioning of SYP-3375 and bromothalonil is between 5:1～1:35, all exists to the co-toxicity coefficient for examination pathogen
More than 120, show that both antibacterial have good synergistic function, especially proportioning at 1:5～1 under this proportioning:
Between 25, potentiation is the most notable.
35 kinds of medicaments of table are to eyespot in apple limb bacterium, Fructus Pruni pseudocerasi gummosis pathogenic bacteria, the toxicity test result of dothiorella gregaria bacterium
Eyespot in apple limb bacterium, Fructus Pruni pseudocerasi gummosis pathogenic bacteria, willow are burst by table 4 SYP-3375 with bromothalonil different proportion mixture
The toxicity test result of infections pathogenic bacteria
From table 3～4, SYP-3375, bromothalonil and mixture preparation thereof, to eyespot in apple limb bacterium, Fructus Pruni pseudocerasi gummosis
Pathogenic bacteria, dothiorella gregaria bacterium are respectively provided with good inhibiting effect, wherein the proportioning of SYP-3375 and bromothalonil 1:2～1:15 it
Between time, to the co-toxicity coefficient for examination pathogen all more than 120, show that both antibacterial have well association under this proportioning
Same potentiation, especially proportioning potentiation between 1:5～1:15 is the most notable.
The 54 kinds of medicaments of the table toxicity test result to Ramulus Persicae rot bacterium
Table 6 SYP-3375 and the bromothalonil different proportion mixture toxicity test result to Ramulus Persicae rot bacterium
From table 5～6, SYP-3375, bromothalonil and mixture preparation thereof, Ramulus Persicae rot bacterium is had well suppression
Effect, when wherein the proportioning of SYP-3375 and bromothalonil is between 2:1～1:35, all exists to the co-toxicity coefficient for examination pathogen
More than 120, show that both antibacterial have good synergistic function, especially proportioning at 1:5～1 under this proportioning:
Between 20, potentiation is the most notable.
The 74 kinds of medicaments of the table toxicity test result to Alternaria mali
Table 8 SYP-3375 and the bromothalonil different proportion mixture toxicity test result to Alternaria mali
From table 7～8, Wu Junzuo, octyl trichlamide and mixture preparation thereof, Alternaria mali is had and well presses down
Make use, when wherein the proportioning of Wu Junzuo and octyl trichlamide is between 2:1～1:25, the co-toxicity coefficient for examination pathogen is all existed
More than 120, show that both antibacterial have good synergistic function, especially proportioning at 1:2～1 under this proportioning:
Between 15, potentiation is the most notable.
The 75 kinds of medicaments of the table toxicity test result to Semen Juglandis anthrax bacteria
Table 8 SYP-3375 and the bromothalonil different proportion mixture toxicity test result to Semen Juglandis anthrax bacteria
From table 7～8, SYP-3375, bromothalonil and mixture preparation thereof, Semen Juglandis anthrax bacteria is had well suppression
Effect, when wherein the proportioning of SYP-3375 and bromothalonil is between 1:2～1:15, all exists to the co-toxicity coefficient for examination pathogen
More than 120, show that both antibacterial have good synergistic function under this proportioning.
Embodiment 1 8% SYP-3375 bromothalonil wettable powder
Component composition is following (each component is by weight percentage):
Preparation method is: above-mentioned each active component, various auxiliary agent and filler are sufficiently mixed in proportion uniformly, mixture warp
Ultra-fine pulverizer disintegrating, prepares 8% SYP-3375 bromothalonil wettable powder.
Embodiment 2 20% SYP-3375 bromothalonil suspending agent
Component composition is following (each component is by weight percentage):
Preparation method is: above-mentioned each active component, various auxiliary agent and water are sufficiently mixed in proportion uniformly, puts into sand milling
Machine, is ground to particle diameter 5 μm, 20% SYP-3375 bromothalonil suspending agent.
Embodiment 3 11% SYP-3375 bromothalonil cream
Component composition is following (each component is by weight percentage):
Preparation method is: above-mentioned each active component, various auxiliary agent and water are sufficiently mixed in proportion, adds in mixing kettle,
The 11% SYP-3375 bromothalonil cream stirred.
Embodiment 4:21% SYP-3375 bromothalonil aqueous emulsion
Component composition is following (each component is by weight percentage):
Preparation method is: above-mentioned each active component, various auxiliary agent are sufficiently mixed in proportion, makes to be dissolved into all and oil phase,
Under high-speed stirred, oil phase is mixed with water, obtains 21% SYP-3375 bromothalonil aqueous emulsion.
Embodiment 5:6% SYP-3375 bromothalonil water dispersible granule
Component composition is following (each component is by weight percentage):
Preparation method is: by above-mentioned each active component, Negel formaldehyde condensation products, wetting agent, disintegrating agent, filler
It is sufficiently mixed in proportion, is crushed to particle diameter 5 μm through jet mill, obtains female powder, mother's powder is sufficiently mixed with sodium lignin sulfonate
Rear pelletize, dry, sieve, thus prepare 6% SYP-3375 bromothalonil water dispersible granule.
Embodiment 6:16% SYP-3375 bromothalonil microemulsion
Component composition is following (each component is by weight percentage):
Preparation method is: above-mentioned each active component, various auxiliary agent are sufficiently mixed in proportion, makes to be dissolved into all and oil phase,
Under high-speed stirred, oil phase is mixed with water, obtains 16% SYP-3375 bromothalonil microemulsion.
Application example 1: field control canker of apple fruit field control effectiveness test
Within 2014, it is carried out example 1 (8% SYP-3375 bromothalonil wettable powder) in Feicheng, Shandong Province to prevent and treat
Apple tree canker field control effectiveness test, verifies the prevention effect of this medicament, effective dose and the safety to apple tree.Test
Method is with reference to " GB/T 17980.98-2004 pesticide field efficacy medicine test criterion (two) bactericidal agent for preventing and treating apple tree canker drug effect
Test ".Test site is in Zhang An village, An Jiazhuan town, and test tree is 17 years tissue regeneration promoting Red Stars, spacing in the rows × line-spacing=3m × 4m, rich water pipe
Reason level is general, extensive management, and tree vigo(u)r is weak, and the trunk disease such as canker, ring spot occurs heavier.
Test medicine and dosage is: 8% SYP-3375 bromothalonil wettable powder 100 times, 200 times, 400 times；3% first
Base thiophanate paste；2.12% copper humate water preparation；40% asomate wettable powder 50 times；Blank, only removes disease
Scar is without chemicals treatment.Tree vigo(u)r scar position close, sick and the close sick scar of sick scar size is selected to be marked.Every community becomes tree in age
At least 5 strains, the newest sick scar at least 8, it is repeated 4 times.
Test and carry out on March 10th, 2014.Medication after scab cleaning, is to use to produce upper conventional to scrape method for the treatment of, the most first
With scraper, canker disease portion being shaved the most clear limit and exceed disease portion slough 0.5-1cm, sick scar otch requires to stand stubble, fusiformis, so
After again with hairbrush by liquid medicine painting disease portion and beyond otch 2-3cm, all moisten with coating position, medicinal liquid is the dirtiest for degree.
Blank only strikes off scab, clear good limit, but is not coated with medicament.
A scab recurrence is respectively investigated when December in 2014 early winter on the 3rd and the early spring on the 15th March in 2015.Record is multiple
Morbidity scar quantity.Simultaneously with vernier caliper measurement maximum healing width (mm) that records disease scar.And medication after scab is cleared up
Statistical is carried out respectively with direct drug injection.
Drug effect statistical analysis technique: calculate pharmacy control efficacy by following equation.
a.
b.
(note: in table 9～table 13, each extension rate, to the solid such as wettable powder, water dispersible granules as shown in table 9 for result
For dosage form, refer to quality multiple, for liquid dosage form, refer to volume multiple).
The pharmacodynamic results of apple tree canker prevented and treated by table 9 8% SYP-3375 bromothalonil wettable powder
Field apple tree canker therapeutic test result sees the above table 9.From table, 8% SYP-3375 bromothalonil is wettable
Property the field control effect that processes of each concentration of powder best, cure rate is more than 80.0%, wherein with 100 times of best results, aobvious
Work is better than other each conventional treatment, and wound healing is fast, smooth.
Application example 2: field apple tree Trunk canker therapeutic test
Within 2014, it is carried out example 2 (20% SYP-3375 bromothalonil suspending agent) in Feicheng, Shandong Province and prevents and treats Fructus Mali pumilae
Tree Trunk canker field control effectiveness test, verifies the prevention effect of this medicament, effective dose and the safety to apple tree.Test
Place is at Wu villages and small towns Wu Cun, and test tree is 19 years raw golden marshall apples, and rich water quality management level is general, and tree vigo(u)r is weak, spacing in the rows × line-spacing
=3 × 4m, branch disease tumor is more.
Design 5 tests to process: 20% SYP-3375 bromothalonil suspending agent 200 times, 400 times, 600 times；3% methyl sulfur
Bacterium spirit paste；43% tebuconazole suspension concentrates 200 times；Blank, only removes sick scar without chemicals treatment.Every 20 strain trees are one
Individual community, is repeated 4 times, and every community selects the position that tree vigo(u)r scar close, sick is close to be marked.
Test and carry out on November 30th, 2014, strike off on trunk and 2 years sick above branches after tumor, be uniformly coated with hairbrush
Smearing medicinal liquid, until trunk base portion, average every strain is coated with medicinal liquid about about 500 milliliters, after having smeared, entirely sets spraying.In coating
Before, the two strain trees in every community by all directions in every orientation, orientation select the raw branches of 5 3-4, paint demarcation the most respectively
The trunk having certain sick tumor of 60-100 square centimeter or branch section.
Before dispenser with each investigation on April 30 in 2015 once, investigate altogether twice.Investigate in each labelling area of each community
Sick tumor quantity, before coating, sick tumor number is as radix, the sick tumor rate of increase of statistics, calculates prevention effect.
Result is as shown in table 10.
Table 10 20% SYP-3375 bromothalonil suspending agent preventing and treating eyespot in apple limb test initial data statistical table
(2015)
20% SYP-3375 bromothalonil suspending agent preventing and treating eyespot in apple limb result of the test sees the above table 10.Can by table
Seeing, 40% 200 times of field control effects of SYP-3375 bromothalonil aqueous emulsion are best, and cure rate, 86.34%, is significantly better than it
He processes, and uses 40% SYP-3375 bromothalonil aqueous emulsion on producing in the way of branch is smeared, and bactericidal effect is good, to ring
Environment pollution is little, is the applicable dosage form of preventing and treating eyespot in apple limb.
Application example 3: field cherry tree bleeding disease therapeutic test
Within 2014, it is carried out example 4 (21% SYP-3375 bromothalonil aqueous emulsion) in Tai'an, Shandong Province and prevents and treats Fructus Pruni pseudocerasi
Tree bleeding disease field control effectiveness test, verifies the prevention effect of this medicament, effective dose and the safety to cherry tree.
Test is carried out in large cherry planting base, the village, Xiyuan, Taishan District, and test tree is 16 years raw red lights, choose tree vigo(u)r suitable,
The plant that the state of an illness is close, control measures are consistent is as examination material.Design 5 tests to process: 21% SYP-3375 bromothalonil water and milk
Agent 200 times, 400 times, 600 times；43% tebuconazole suspension concentrates 200 times；3% thiophanate-methyl paste；5 Baume degrees lime sulfurs；
Blank, only removes colloid without chemicals treatment.Every 4 strain trees are a community, are repeated 4 times, random alignment.
Remove disease portion dead bark, breach and blob of viscose on April 25th, 2014 (before rainy season starts), indulge at sick cortex with scraper
Horizontal cut, then smears test medicine with scrub-brush, and scope exceeds disease portion 3～5cm.It is spaced and repastes once for 7 days.
Every community is coated with 20 gummosis scabs, often processes and amounts to 80.It is coated with latter 60 days to observe and cures (no longer gummosis) number and stream
Healing state (stopping gummosis, the most dead tissue is replaced by cambium) at glue, using cure rate as indicator-specific statistics prevention effect.
Result is as shown in table 11.
Table 11 field cherry tree bleeding disease therapeutic test result
Field cherry tree bleeding disease therapeutic test the results are shown in Table 11.21% 200 times of fields of SYP-3375 bromothalonil aqueous emulsion
Between prevention effect best, cure rate, 85.0%, is significantly better than other each conventional treatment, and wound healing is fast, smooth.
Application example 4: field Ramulus Persicae rot therapeutic test
(6% SYP-3375 bromothalonil is water-dispersible within 2014, to be carried out example 5 in peach garden, the village, Xiyuan, Tai'an, Shandong Province
Granule) preventing and treating Ramulus Persicae rot field control effectiveness test, verify the prevention effect of this medicament, effective dose and the safety to peach tree
Property.
Test is carried out in peach garden, the village, Xiyuan, and test tree is early raw for storehouse side, chooses that tree vigo(u)r is suitable, the state of an illness is close, control measures
Consistent plant is as examination material.Design 5 tests to process: 6% SYP-3375 bromothalonil water dispersible granule 1000 times, 1500
Again, 2000 times；43% tebuconazole suspension concentrates 2500 times；50% carbendazol wettable powder 600 times；Blank.Every 4 strain trees
It is a community, is repeated 4 times, community random alignment, and sign of listing.
Disease investigation and preventive effect calculate 15d and 40d after last 1 dispenser respectively, investigation, calculate strain disease branch rate and deadwood
Rate, calculates effect of chemical control simultaneously.
Starting the 1st dispenser in their early stage, power driven sprayer carries out foliar spray process, each dispenser interval 10～
15d.After last dispenser and medicine thereof, 20d investigates sickness rate and the disease index of each process community, during investigation every strain tree eastern,
South, west, direction, 4, north totally 80 shoots (20, every direction), calculate Investigate incidence and disease index, use DMRT method statistics
Analyze result of the test, simultaneously investigation peach tree whether affected by institute's dispenser agent and produced poisoning, and the symptom type of poisoning and
Extent of injury.
The field severity Scaling of Ramulus Persicae rot divides 6 grades, specific as follows:
0 grade, Herb is anosis；1 grade, peach branch scab is around the 50% of branch girth, and 10% is wilting with lower blade；3 grades,
Peach branch scab is wilting around 51%～100%, 11%～30% blade of branch girth；5 grades, peach branch scab cincture
Branch 1 week, 31%～50% blade is wilting, and branch is slightly withered；7 grades, peach branch scab around branch 1 week, 51%～
70% blade is wilting, fallen leaves, and branch moderate is withered；9 grades, Ramulus Persicae branch scab cincture branch 1 week, 70% withers with blade
Withering, fall leaves, branch is withered.Disease index, preventive effect etc. are with reference to the method for Huang Guoyang, and data statistics utilizes DPS software to complete.
a.
b.
The results are shown in Table 12.
Table 12 field Ramulus Persicae rot prevents and treats result
Field Ramulus Persicae rot therapeutic test the results are shown in Table 12.6% SYP-3375 bromothalonil water dispersible granule 1000 times,
1500 times of field control effects are best, and last prevention effect, all more than 90.0%, is significantly better than other and respectively processes, and to Fructus Persicae
Tree safety.
Application example 5: the field control Semen Juglandis anthrax test of pesticide effectiveness
Within 2014, it is carried out example 6 (16% fourth in Taishan District of Taian City, Shandong Province Shandong Fruit-tree Inst. five orchard
Fragrant bacterium ester bromothalonil microemulsion) preventing and treating Semen Juglandis anthrax field control effectiveness test, verify the prevention effect of this medicament, effective dose
And the safety to walnut.
This garden is sandy loam, and test tree is susceptible variety " the fragrant tinkling of pieces of jade ", the age of tree 14 years, spacing in the rows × line-spacing=3 × 5m, rich water pipe
Reason level is general, and anthrax occurs heavier over the years, chooses tree vigo(u)r is suitable, the state of an illness is close, control measures are consistent plant as examination
Material.Design 5 tests to process: 16% SYP-3375 bromothalonil microemulsion 1000 times, 1500 times, 2000 times；43% Tebuconazole
Suspending agent 3000 times；50% carbendazol wettable powder 600 times；Blank.May 27, June 11, June 25, July
9 days, July 23, August 7 carry out spraying medicine, dispenser 6 times altogether.Every 4 strain trees are a community, are repeated 4 times, community random alignment.
Investigation morbidity radix during dispenser for the first time on May 27.During dispenser, investigation is not fallen ill for the first time, therefore morbidity radix is
Zero.August 7 (after the 5th medicine 14 days), August 21 (after the 6th medicine 14 days) respectively investigate an incidence.
Strain test tree is all investigated in every community 2.Investigation tree is upper, total fruit number of (landing) and anthrax fruit number, statistics disease under tree
Really rate, calculates prevention effect.
Drug effect statistical analysis technique: calculate pharmacy control efficacy by following equation；DPS software is utilized to carry out the new multipole of Duncan poor
Check its diversity (P≤0.05).
a.
b.
Result is as shown in table 13.
Table 13 field Semen Juglandis anthrax prevents and treats result
Field Semen Juglandis anthracnose treatment result of the test is shown in Table 13.16% SYP-3375 bromothalonil microemulsion 1000 times,
1500 times of field control effects are best, and last investigation cure rate, all more than 90.0%, is significantly better than other and respectively processes, and right
Walnut safety.
Claims (10)
1. one kind contains SYP-3375 and the bactericidal composition of bromothalonil, it is characterised in that: effective ingredient is SYP-3375 and bromine bacterium
Nitrile, SYP-3375 is 5:1～1:35 with the weight ratio of bromothalonil.
It is the most according to claim 1 containing SYP-3375 with the bactericidal composition of bromothalonil, it is characterised in that: described containing Flos Caryophylli
The bactericidal composition of bacterium ester and bromothalonil is made up of the component of following weight portion: Flos Caryophylli polyester 1%～80%, bromothalonil
1%～80%, surplus is to allow in Pesticide Science to use and acceptable adjuvant；Described Pesticide Science allows use and can connect
The adjuvant being subject to is selected from solvent, penetrating agent, dispersant, diffusant, disintegrating agent, emulsifying agent, stabilizer, wetting agent, defoamer, antifreeze
Agent, or other contributes to the stable of effective ingredient drug effect and allowing of playing makes used additives.
It is the most according to claim 1 containing SYP-3375 with the bactericidal composition of bromothalonil, it is characterised in that: described sterilization group
The dosage form of compound is wettable powder, suspending agent, cream, aqueous emulsion, water dispersible granules or microemulsion.
4. according to according to any one of claims 1 to 3 containing SYP-3375 and the bactericidal composition of bromothalonil, its feature exists
In: the dosage form of described bactericidal composition is wettable powder, is made up of the component of following percetage by weight:
5. according to according to any one of claims 1 to 3 containing SYP-3375 and the bactericidal composition of bromothalonil, its feature exists
In: the dosage form of described bactericidal composition is suspending agent, is made up of the component of following percetage by weight:
6. according to according to any one of claims 1 to 3 containing SYP-3375 and the bactericidal composition of bromothalonil, its feature exists
In: the dosage form of described bactericidal composition is cream, is made up of the component of following percetage by weight:
7. according to according to any one of claims 1 to 3 containing SYP-3375 and the bactericidal composition of bromothalonil, its feature exists
In: the dosage form of described bactericidal composition is aqueous emulsion, water dispersible granules or microemulsion,
When dosage form is aqueous emulsion, it is made up of the component of following percetage by weight:
When dosage form is water dispersible granules, it is made up of the component of following percetage by weight:
When dosage form is microemulsion, it is made up of the component of following percetage by weight:
8. according to the bactericidal composition containing SYP-3375 and bromothalonil according to any one of claim 1～7 preventing and treating fruit tree,
Application in forest branch, vegetable disease, or as fruit tree, forest grafting, beta pruning sealing compound in application.
Application the most according to claim 8, it is characterised in that: described disease is selected from canker of apple fruit, Fructus Mali pumilae branch wheel stricture of vagina
Disease, Fructus Pruni pseudocerasi bleeding disease, peach gummosis, dothiorella gregaria, Ramulus Persicae rot, alternaria leaf spot of apple, Semen Juglandis anthrax.
Application the most according to claim 9, it is characterised in that: when preventing and treating Valsa mali, SYP-3375 and bromine bacterium
The weight ratio of both nitriles is preferred with 5:1～1:35, and more preferably, 1:10 is optimal for 1:5～25；When preventing and treating eyespot in apple limb bacterium,
SYP-3375 is preferred with 1:2～15 with the weight ratio of both bromothalonils, and more preferably, 1:10 is optimal for 1:5～15；At preventing and treating Fructus Pruni pseudocerasi gummosis
During pathogenic bacteria, SYP-3375 is preferred with 1:2～15 with the weight ratio of both bromothalonils, and more preferably, 1:10 is optimal for 1:5～15；In preventing and treating
During dothiorella gregaria bacterium, SYP-3375 is preferred with 1:2～15 with the weight ratio of both bromothalonils, and more preferably, 1:10 is for 1:5～15
Good；When preventing and treating Ramulus Persicae rot bacterium, SYP-3375 is preferred with 2:1～1:35 with the weight ratio of both bromothalonils, and 1:5～20 is more
Good, 1:10 is optimal；When preventing and treating Alternaria mali, the weight ratio of SYP-3375 and both bromothalonils is with 2:1～1:25
Being preferred, more preferably, 1:5 is optimal for 1:2～15；When preventing and treating Semen Juglandis anthrax bacteria, the weight ratio of SYP-3375 and both bromothalonils with
1:2～15 is preferred, and 1:10 is optimal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610595215.1A CN106172404B (en) | 2016-07-26 | 2016-07-26 | A kind of bactericidal composition and its application containing coumoxystrobin and bromothalonil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610595215.1A CN106172404B (en) | 2016-07-26 | 2016-07-26 | A kind of bactericidal composition and its application containing coumoxystrobin and bromothalonil |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106172404A true CN106172404A (en) | 2016-12-07 |
| CN106172404B CN106172404B (en) | 2018-04-17 |
Family
ID=57495768
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610595215.1A Active CN106172404B (en) | 2016-07-26 | 2016-07-26 | A kind of bactericidal composition and its application containing coumoxystrobin and bromothalonil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106172404B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047586A (en) * | 2016-11-02 | 2017-08-18 | 塔里木大学 | A kind of method of preventing and treating Xinjiang Cold region apple jujube black spot |
| CN107980777A (en) * | 2017-12-07 | 2018-05-04 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition containing bromothalonil and eugenol |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690490A (en) * | 2009-10-14 | 2010-04-07 | 成都皇牌作物科学有限公司 | Bactericidal composition compounded by bromothalonil and methoxy acrylic acid esters |
| CN104430357A (en) * | 2014-11-10 | 2015-03-25 | 吉林省八达农药有限公司 | Bactericide composition containing coumoxystrobin with synergistic effect |
| CN104770368A (en) * | 2015-04-16 | 2015-07-15 | 山东省果树研究所 | Bactericidal composition containing coumoxystrobin and xinjunan and application thereof |
| CN105076141A (en) * | 2014-11-17 | 2015-11-25 | 吉林省八达农药有限公司 | Composition containing coumoxystrobin and related bactericide |
-
2016
- 2016-07-26 CN CN201610595215.1A patent/CN106172404B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690490A (en) * | 2009-10-14 | 2010-04-07 | 成都皇牌作物科学有限公司 | Bactericidal composition compounded by bromothalonil and methoxy acrylic acid esters |
| CN104430357A (en) * | 2014-11-10 | 2015-03-25 | 吉林省八达农药有限公司 | Bactericide composition containing coumoxystrobin with synergistic effect |
| CN105076141A (en) * | 2014-11-17 | 2015-11-25 | 吉林省八达农药有限公司 | Composition containing coumoxystrobin and related bactericide |
| CN104770368A (en) * | 2015-04-16 | 2015-07-15 | 山东省果树研究所 | Bactericidal composition containing coumoxystrobin and xinjunan and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047586A (en) * | 2016-11-02 | 2017-08-18 | 塔里木大学 | A kind of method of preventing and treating Xinjiang Cold region apple jujube black spot |
| CN107980777A (en) * | 2017-12-07 | 2018-05-04 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition containing bromothalonil and eugenol |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106172404B (en) | 2018-04-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101669486B (en) | Benzolkresoxim-methyl containing bactericide composition | |
| CN104770368B (en) | Bactericidal composition and its application containing SYP-3375 and octyl trichlamide | |
| CN101548677B (en) | Sterilizing compound of ethirimol and triazole | |
| CN101755847A (en) | Metarhiziumanisopliae compound insecticide composition and use thereof | |
| CN104322558A (en) | Sterilization composition and applications thereof | |
| CN103814923A (en) | Dispersible oil suspending agent containing pyraclostrobin | |
| CN101326918B (en) | Water-soluble granular formulation containing Imidacloprid, and preparation method and use thereof | |
| CN104170825A (en) | Composite herbicide containing pyrithiobac-sodium, fluorochloridone and haloxyfop-R-methyl, and application thereof | |
| CN101836630A (en) | Pesticide composition containing amino-oligosaccharide and kresoxim-methyl and use method and application thereof | |
| CN106172425B (en) | A kind of bactericidal composition and its application containing octyl trichlamide and propiconazole | |
| CN102365956A (en) | Sterilization composition containing fluopicolide and methoxyl acrylic acid ester compounds | |
| CN106172404B (en) | A kind of bactericidal composition and its application containing coumoxystrobin and bromothalonil | |
| CN102027936A (en) | Water dispersible granule containing kresoxim-methyl and tebuconazole | |
| CN102388890B (en) | Bactericidal composition containing SYP-3375 and cyprodinil | |
| CN101861868B (en) | Pesticide compound containing penoxsulam and quinclorac and application thereof | |
| CN101658173A (en) | Sterilization composition containing tetraconazole | |
| CN103918686A (en) | Dispersing oil suspending agent containing thiophanate-methyl | |
| CN114617127B (en) | Pesticide sterilization composition and application thereof | |
| CN110896948A (en) | Insecticidal composition containing d-limonene | |
| CN103651452A (en) | Bactericidal composition containing octyl trichlamide and carbendazim and application of bactericidal composition | |
| CN103168781B (en) | Compound sterilizing agent containing fluopicolide and fludioxonil and application of sterilizing agent | |
| CN104145983A (en) | Dispersible oil suspension containing sulfoxaflor | |
| CN110547295B (en) | Moss prevention and treatment agent and application thereof | |
| CN109430264B (en) | Bactericide composition containing terpene alcohol and chitosan, and preparation method and application thereof | |
| CN106212472B (en) | A kind of bactericidal composition and its application containing octyl trichlamide and penconazole |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Fan Kun Inventor after: Jin Xiaodi Inventor after: Fu Li Inventor after: Qu Jianlu Inventor after: Qin Chenglai Inventor before: Fan Kun Inventor before: Fu Li Inventor before: Qu Jianlu Inventor before: Qin Chenglai |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |