CN106135629A - A kind of extracting method of plant edible food leaf protein - Google Patents
A kind of extracting method of plant edible food leaf protein Download PDFInfo
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- CN106135629A CN106135629A CN201510112276.3A CN201510112276A CN106135629A CN 106135629 A CN106135629 A CN 106135629A CN 201510112276 A CN201510112276 A CN 201510112276A CN 106135629 A CN106135629 A CN 106135629A
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- 101710138460 Leaf protein Proteins 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 47
- 235000013305 food Nutrition 0.000 title claims abstract description 20
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 48
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 44
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 44
- 241000196324 Embryophyta Species 0.000 claims abstract description 30
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 22
- 239000004310 lactic acid Substances 0.000 claims abstract description 22
- 239000011780 sodium chloride Substances 0.000 claims abstract description 22
- 238000010009 beating Methods 0.000 claims abstract description 21
- 238000001914 filtration Methods 0.000 claims abstract description 16
- 238000010438 heat treatment Methods 0.000 claims abstract description 15
- 230000001376 precipitating effect Effects 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 11
- 239000002253 acid Substances 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 239000006210 lotion Substances 0.000 claims abstract description 7
- 238000004806 packaging method and process Methods 0.000 claims abstract description 7
- 238000001556 precipitation Methods 0.000 claims abstract description 7
- 238000000746 purification Methods 0.000 claims abstract description 7
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 239000006228 supernatant Substances 0.000 claims abstract description 7
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000004108 freeze drying Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 2
- 238000005265 energy consumption Methods 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 description 20
- 102000004169 proteins and genes Human genes 0.000 description 20
- 239000002002 slurry Substances 0.000 description 8
- 241000219823 Medicago Species 0.000 description 6
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000007859 condensation product Substances 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000001814 protein method Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108010049994 Chloroplast Proteins Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000010624 Medicago sativa Nutrition 0.000 description 1
- 102000008934 Muscle Proteins Human genes 0.000 description 1
- 108010074084 Muscle Proteins Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Abstract
The invention discloses the extracting method of a kind of plant edible food leaf protein, comprise the steps: a, pull an oar, squeeze the juice: fresh plant stem-leaf is cleaned, with beater making beating, and squeeze the juice and obtain juice, using NaCl solution to pull an oar in described beating process, the concentration of described NaCl solution is 0.2-1%；B, heating: the juice in step a is heated to 70-90 DEG C, be incubated 5-9min, be then quickly cooled to 40-45 DEG C；C, acid adding precipitation: adding lactic acid in the juice after cooling, precipitating 1-3h, the pH value of described lactic acid is 3.5-4, and the volume dosage of described lactic acid is the 6-8% of juice volume；The albumen lotion precipitating is carried out filtering separation, makes albumen wet cake by d, Purification by filtration: filter off supernatant liquor；E, drying, packaging.The extracting method of the plant edible food leaf protein that the present invention provides, can extract leaf protein fast and efficiently, and energy consumption is low.
Description
Technical field
The present invention relates to vegetable protein extractive technique field, be specifically related to the extraction of a kind of plant edible food leaf protein
Method.
Background technology
Protein is one of base substance constituting plant cell, in growing process, aerial part stem
Leaf cell constantly has the synthesis of protein, for building the needs of new cell tissue and organ, this part egg
White matter is referred to as plant leaf protein (Leaves Protein Concentrates is called for short LPC), and they belong to
In functional protein class.Owing to being to there is axis, Ye Zhong, so being the reproducible egg of a kind of maximum
White matter resource.Plant leaf protein contains 17～18 kinds of amino acid, contains 8 kinds of amino acid necessary to human body,
Possibly together with vitamin, do not contain saturated fatty acid and cholesterol from plant leaf protein unlike animal muscle protein.
With the raising of the development of food industry and living standards of the people, pollution-free food is increasingly by people's
Like, from plant, extract leaf protein, there is considerable application prospect.At present, the extraction of plant leaf protein
Method mainly has: 1) heating aggregation method: plant is smashed to pieces filtration filter after extract juice, use steam heating or
Direct-fired way makes protein condense.The particle that this condensation product is formed is big, it is easy to separating and filtering,
And the particle of cohesion is bigger, consolidation is also got in cohesion, and the danger gone mouldy is less.In order to make leaf protein fully extract
Out, typically use fractional heating, i.e. first add to 60 DEG C～70 DEG C, be quickly cooled to 40 DEG C, leach
Condensation product is chloroplast protein, does not all reach the requirement of edible protein at the aspect such as mouthfeel, color, only
Can serve as feed；It is then again heated to 80 DEG C～90 DEG C, and continues 2～4 minutes, leach condensation product main
For cytoplasm protein, can be as edible protein.The main deficiency of the method is that energy consumption is high, does not meets and currently advocates
The low-carbon (LC) theory led, and under high temperature, protein easily hardens, and has a strong impact on product quality；2) acidifying heating or
Alkalization heating aggregation method: wherein alkali cohesion is to add a certain amount of alkali in juice after the press, makes juice
PH value reaches 4.0～6.0, and then heating makes leaf protein condense out.The method can obtain more coagulating
Polymers, but condensation product quality softness is not readily separated；3) fermentation method: juice is fermented under anoxic conditions two days
Left and right, utilizing Bacillus acidi lactici to produce lactic acid makes leaf protein condense out.The method saves the energy, but owing to sending out
The ferment time is long, and the enzymolysis of leaf protein extends, and can cause certain nutritive loss.
It is, therefore, desirable to provide a kind of new method for extracting leaf protein, meet and both saved the energy, leaf can be increased again
Protein extraction amount, and ensure the requirement of leaf protein quality.
Content of the invention
It is an object of the invention to overcome disadvantage mentioned above, a kind of leaf protein of plant edible food fast and efficiently is provided
Extracting method, and the method energy consumption is low.
The technical scheme is that
The extracting method of a kind of plant edible food leaf protein, comprises the steps:
A, pull an oar, squeeze the juice: fresh plant stem-leaf is cleaned, with beater making beating, and squeeze the juice and obtain juice,
Using NaCl solution to pull an oar in described beating process, the concentration of described NaCl solution is 0.2-1%；
B, heating: the juice in step a is heated to 70-90 DEG C, be incubated 5-9min, then quickly cool down
To 40-45 DEG C；
C, acid adding precipitation: add lactic acid in the juice after cooling, precipitate 1-3h, the pH value of described lactic acid
For 3.5-4, the volume dosage of described lactic acid is the 6-8% of juice volume；
The albumen lotion precipitating is carried out filtering separation, makes by d, Purification by filtration: filter off supernatant liquor
Albumen wet cake；
E, drying, packaging.
Further, in described step a, the concentration of described NaCl solution is 0.5%.
Further, in described step a, the volume ratio 1: 1-5 of described raw material and described NaCl solution.
Further, the technique of described step b is, juice is heated to 70 DEG C, is incubated 7min, then soon
Speed is cooled to 45 DEG C.
Further, in described step e, use and be spray-dried or freeze-drying.
Further, in described step d, the water content of albumen wet cake is 55-58%.
Further, in described step e, baking temperature is 105 DEG C.
Further, in described step a, pull an oar after the segment that plant stem-leaf is cut into 0.5-1cm.
The extracting method of the plant edible food leaf protein that the present invention provides, has the advantage that
1st, the extracting method of the plant edible food leaf protein that the present invention provides, uses NaCl solution in beating process
Making beating, can promote the dissolving of protein, increases the extracted amount of protein, and dilute NaCl solution is because of salt ion simultaneously
Be combined with protein portion, the advantage of protected protein not mutability, thus increase the stability of protein.
2nd, the extracting method of the plant edible food leaf protein that the present invention provides, by heating means and acid adding intermediate processing
In conjunction with, by optimizing each technique, shortening the sedimentation time of leaf protein, the protein obtaining is easily separated；And
In heating steps, once heat, saved energy consumption.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that retouched
The embodiment stated is only a part of embodiment of the present invention, rather than whole embodiments.Based in the present invention
Embodiment, those of ordinary skill in the art obtained under the premise of not making creative work all its
His embodiment, broadly falls into the scope of protection of the invention.
Embodiment 1
Yield of alfalfa is high, nutritious, best in quality, is the artificial grass of China's cultivated area maximum.
Medicago sativa l. PC gross protein value is generally 43-60%, and content is up to 70% sometimes, its amino acid content and
Composition is better than other vegetable proteins.Therefore, this test select alfalfa extract plant leaf protein.
Extracting method is as follows:
A, pull an oar, squeeze the juice: carry out beating with beater after fresh alfalfa stem and leaf is cut into the segment of 0.5-1cm
Slurry, making beating uses the dilute NaCl solution of 0.2%, then with squeezer, slurry after making beating is squeezed out juice；Former
Material and the volume ratio 1: 5 of NaCl solution；
B, heating: the juice in step a is heated to 80 DEG C and carries out solidifying and being sterilized, and be incubated 5min,
Then it is quickly cooled to 40 DEG C；
C, acid adding precipitation: adding lactic acid in the juice after cooling, precipitating 2h, the pH value of lactic acid is 4,
And the volume dosage of lactic acid is the 6% of juice volume；
The albumen lotion precipitating is carried out filtering separation, makes by d, Purification by filtration: filter off supernatant liquor
Albumen wet cake, the water content of control albumen wet cake is 55%；
E, drying, packaging, drying process uses and is spray-dried, and baking temperature is 100 DEG C.
Measurement leaf protein yield, and measure the content of protein.
Wherein, leaf protein yield=fresh sample gained leaf protein does grams/fresh sample grams × 100%；Protein content
Assay method is according to GB/T6432-1994.
Embodiment 2
Method for extracting leaf protein is as follows:
A, pull an oar, squeeze the juice: carry out beating with beater after fresh alfalfa stem and leaf is cut into the segment of 0.5-1cm
Slurry, making beating uses the dilute NaCl solution of 0.5%, then with squeezer, slurry after making beating is squeezed out juice；Former
Material and the volume ratio 1: 2 of NaCl solution；
B, heating: the juice in step a is heated to 70 DEG C and carries out solidifying and being sterilized, and be incubated 7min,
Then it is quickly cooled to 45 DEG C；
C, acid adding precipitation: adding lactic acid in the juice after cooling, precipitating 2.5h, the pH value of lactic acid is 3.6,
And the volume dosage of lactic acid is the 7% of juice volume；
The albumen lotion precipitating is carried out filtering separation, makes by d, Purification by filtration: filter off supernatant liquor
Albumen wet cake, the water content of control albumen wet cake is 58%；
E, drying, packaging, drying process uses freeze-drying, and baking temperature is 105 DEG C.
Measurement leaf protein yield, and measure the content of protein.
Embodiment 3
Method for extracting leaf protein is as follows:
A, pull an oar, squeeze the juice: carry out beating with beater after fresh alfalfa stem and leaf is cut into the segment of 0.5-1cm
Slurry, making beating uses the dilute NaCl solution of 0.8%, then with squeezer, slurry after making beating is squeezed out juice；Former
Material and the volume ratio 1: 3 of NaCl solution；
B, heating: the juice in step a is heated to 90 DEG C and carries out solidifying and being sterilized, and be incubated 9min,
Then it is quickly cooled to 43 DEG C；
C, acid adding precipitation: adding lactic acid in the juice after cooling, precipitating 1h, the pH value of lactic acid is 3.8,
And the volume dosage of lactic acid is the 8% of juice volume；
The albumen lotion precipitating is carried out filtering separation, makes by d, Purification by filtration: filter off supernatant liquor
Albumen wet cake, the water content of control albumen wet cake is 56%；
E, drying, packaging, drying process uses freeze-drying, and baking temperature is 103 DEG C.
Measurement leaf protein yield, and measure the content of protein.
Embodiment 4
Method for extracting leaf protein is as follows:
A, pull an oar, squeeze the juice: carry out beating with beater after fresh alfalfa stem and leaf is cut into the segment of 0.5-1cm
Slurry, making beating uses the dilute NaCl solution of 1%, then with squeezer, slurry after making beating is squeezed out juice；Raw material
Volume ratio 1: 1 with NaCl solution；
B, heating: the juice in step a is heated to 75 DEG C and carries out solidifying and being sterilized, and be incubated 8min,
Then it is quickly cooled to 42 DEG C；
C, acid adding precipitation: adding lactic acid in the juice after cooling, precipitating 1.5h, the pH value of lactic acid is 4,
And the volume dosage of lactic acid is the 6% of juice volume；
The albumen lotion precipitating is carried out filtering separation, makes by d, Purification by filtration: filter off supernatant liquor
Albumen wet cake, the water content of control albumen wet cake is 57%；
E, drying, packaging, drying process uses freeze-drying, and baking temperature is 110 DEG C.
Measurement leaf protein yield, and measure the content of protein.
Embodiment 5-6
Solution used in beating process, on the basis of embodiment 2, is respectively modified as by method for extracting leaf protein
The NaCl solution of 5% and water, other technologies feature invariant, obtain embodiment 5-6.
Embodiment 7-8
Temperature retention time in step b, on the basis of embodiment 2, is respectively modified as by method for extracting leaf protein
3min, 11min, other technologies feature invariant, obtain embodiment 7-8.
Embodiment 9
Step b juice temperature, on the basis of embodiment 2, is cooled fast to 50 DEG C by method for extracting leaf protein,
Other technologies feature invariant.
Embodiment 10
Step b juice temperature, on the basis of embodiment 2, is heated to 100 DEG C by method for extracting leaf protein, its
His technical characteristic is constant.
Embodiment 11-12
Method for extracting leaf protein is on the basis of embodiment 2, and control albumen wet cake water content is revised as the 50%th, 60%,
Other technologies feature invariant, obtains embodiment 11-12.
Measurement leaf protein yield and protein content result are as follows:
| Embodiment | Leaf protein yield (%) | Protein content (g/kg) |
| Embodiment 1 | 7.53 | 647.7 |
| Embodiment 2 | 7.64 | 652.3 |
| Embodiment 3 | 7.58 | 650.7 |
| Embodiment 4 | 7.62 | 648.4 |
| Embodiment 5 | 7.35 | 631.9 |
| Embodiment 6 | 6.72 | 621.4 |
| Embodiment 7 | 7.25 | 629.6 |
| Embodiment 8 | 7.41 | 641.5 |
| Embodiment 9 | 7.38 | 637.8 |
| Embodiment 10 | 7.46 | 645.1 |
| Embodiment 11 | 7.60 | 653.2 |
| Embodiment 12 | 7.65 | 651.7 |
The leaf protein yield recording above-described embodiment and protein content are analyzed understanding, carry in the present invention
In the method for extracting leaf protein of confession, concentration, the juice of NaCl solution heat the temperature retention time in technique, juice
Chilling temperature etc. bigger on the extraction process impact of leaf protein.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all the present invention's
Any modification of being made in protection domain, equivalent etc., should be included within the scope of the present invention.
Claims (8)
1. the extracting method of a plant edible food leaf protein, it is characterised in that described extracting method comprises the steps:
A, pull an oar, squeeze the juice: fresh plant stem-leaf is cleaned, with beater making beating, and squeeze the juice and obtain juice,
Using NaCl solution to pull an oar in described beating process, the concentration of described NaCl solution is 0.2-1%；
B, heating: the juice in step a is heated to 70-90 DEG C, be incubated 5-9min, then quickly cool down
To 40-45 DEG C；
C, acid adding precipitation: add lactic acid in the juice after cooling, precipitate 1-3h, the pH value of described lactic acid
For 3.5-4, the volume dosage of described lactic acid is the 6-8% of juice volume；
The albumen lotion precipitating is carried out filtering separation, makes by d, Purification by filtration: filter off supernatant liquor
Albumen wet cake；
E, drying, packaging.
2. the extracting method of plant edible food leaf protein according to claim 1, it is characterised in that described step
In a, the concentration of described NaCl solution is 0.5%.
3. the extracting method of plant edible food leaf protein according to claim 2, it is characterised in that described step
In a, the volume ratio 1: 1-5 of described raw material and described NaCl solution.
4. the extracting method of plant edible food leaf protein according to claim 1, it is characterised in that described step
The technique of b is, juice is heated to 70 DEG C, is incubated 7min, is then quickly cooled to 45 DEG C.
5. the extracting method of plant edible food leaf protein according to claim 1, it is characterised in that described step
In e, use and be spray-dried or freeze-drying.
6. the extracting method of plant edible food leaf protein according to claim 1, it is characterised in that described step
In d, the water content of albumen wet cake is 55-58%.
7. the extracting method of plant edible food leaf protein according to claim 5, it is characterised in that described step
In e, baking temperature is 105 DEG C.
8. the extracting method of plant edible food leaf protein according to any one of claim 1 to 7, its feature exists
In, in described step a, pulling an oar after the segment that plant stem-leaf is cut into 0.5-1cm.
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| CN201510112276.3A CN106135629A (en) | 2015-03-16 | 2015-03-16 | A kind of extracting method of plant edible food leaf protein |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111616256A (en) * | 2020-06-15 | 2020-09-04 | 宁翠平 | Method for extracting leaf-eating vegetable protein |
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|---|---|---|---|---|
| CN101371678A (en) * | 2008-09-04 | 2009-02-25 | 甘肃省农业科学院畜草品种改良研究所 | Method for directly separating and extracting leaf protein of heart trefoil for feeding and eating |
| CN102090500A (en) * | 2010-06-10 | 2011-06-15 | 长沙富仁投资管理有限公司 | Extraction method of medicinal and edible plant leaf protein and SOD (Super Oxide Dlsmutase) |
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2015
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101371678A (en) * | 2008-09-04 | 2009-02-25 | 甘肃省农业科学院畜草品种改良研究所 | Method for directly separating and extracting leaf protein of heart trefoil for feeding and eating |
| CN102090500A (en) * | 2010-06-10 | 2011-06-15 | 长沙富仁投资管理有限公司 | Extraction method of medicinal and edible plant leaf protein and SOD (Super Oxide Dlsmutase) |
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| 刘青广: "苜蓿叶蛋白的提取及深加工技术的研究", 《中国优秀硕士学位论文全文数据库工程科技I辑》 * |
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| CN111616256A (en) * | 2020-06-15 | 2020-09-04 | 宁翠平 | Method for extracting leaf-eating vegetable protein |
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Effective date of registration: 20200806 Address after: Huangjin formation, Yuehua village, Yuelu District, Changsha City, Hunan Province Applicant after: Zhao Jianhong Address before: 410000 new Kang Road, Ningxiang economic and Technological Development Zone, Hunan province (third housing, 609 incubators, Hong Kong) Applicant before: HUNAN DONGTING AGRICULTURE SCIENCE AND TECHNOLOGY Co.,Ltd. |
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Application publication date: 20161123 |
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