CN106135298A - Radix Carpesii extract and its preparation method and application - Google Patents
Radix Carpesii extract and its preparation method and application Download PDFInfo
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- CN106135298A CN106135298A CN201610604491.XA CN201610604491A CN106135298A CN 106135298 A CN106135298 A CN 106135298A CN 201610604491 A CN201610604491 A CN 201610604491A CN 106135298 A CN106135298 A CN 106135298A
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- radix carpesii
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses Radix Carpesii extract and its preparation method and application.The Radix Carpesii antibacterial of the present invention is environmentally safe, the material that reactive compound in Radix Carpesii antibacterial is naturally-occurring, derive from nature, its intrinsic participation energy and material cyclic metabolic pathway has been defined in long-term evolutionary process, so being applied in environment or noresidue on plant, biological concentration will not be caused;Comparatively safe to non-target organism;The mechanism of action of phytopathogen is different from conventional pesticide, uses rear pathogen to be difficult to it and develop immunity to drugs, pathogen is also had retarding action to the Drug resistance of conventional sterilization agent.
Description
Technical field
The present invention relates to plant extract and application thereof, be specifically related to Radix Carpesii extract and its preparation method and application.
Background technology
Since the forties in 20th century, chemical pesticide is because it is wide in variety, instant effect, easy to use, lower-price characteristic
And in pesticide market, occupy leading position.But, due to people to the unreasonable use of chemical pesticide and pesticide inherently
Shortcoming.Chemical pesticide is caused in use to create the problems such as environmental pollution, person poultry poisoning, pesticide residues.Along with people are raw
The raising of bioplasm amount and the enhancing of environmental consciousness. person poultry safety, biological pesticide pollution-free, low toxicity, low-residual are become by exploitation
New focus for pesticide field research.Botanical pesticide is as a part for biorational pesticide, and its developmental research is always
One of hot subject of novel pesticide research.Botanical pesticide derives from nature, can degrade at nature. typically do not pollute the environment
And agricultural product, in environment and human body, the probability of accumulation toxicity is little, comparatively safe to people and domestic animal, injures pest natural enemy
Little, there is the feature of low toxicity, low-residual, it is possible to keeping the high-quality of agricultural product, therefore botanical pesticide has wide market.
In recent years, the most many scholars investigated and have studied antibacterial, the bactericidal activity of some plant extracts.Some
Research shows, plant source presses down (killing) fungus matter and may act on multiple structures of somatic cells, and microorganism produces the risk of resistance relatively
Little.
Botanical fungicide has effect wide spectrum, rapid without sharp crest Vertebrate toxicity or phytotoxicity, environment degradable
And aboundresources, the advantage such as renewable, therefore the development and application to botanical fungicide has boundless prospect.
Summary of the invention
In order to solve the deficiencies in the prior art, the invention provides Radix Carpesii extract and its preparation method and application.
The technical scheme is that Radix Carpesii extract, the root of Radix Carpesii, stem, leaf and/or seed make after extracting
?.
The preparation method of Radix Carpesii extract, comprises the following steps: step one, by dry Radix Carpesii root, stem, leaf and/
Or seed pulverizing, add 80%~the ethanol of 95% extracts, collect filtrate, obtain Radix Carpesii crude extract;Step 2, Radix Carpesii is thick
Extract extracts through ethyl acetate, obtains Radix Carpesii antimicrobial extract.
Further improvement of the present invention includes:
Described step one is repeatedly extracted and is repeatedly no longer changed to lixiviating solution color, and merging filtrate obtains extracting solution, this is carried
Take liquid and be condensed into dilute extractum, obtain Radix Carpesii crude extract.
In described step 2, Radix Carpesii crude extract ester extract after ethyl acetate extracts sloughs color through macroporous adsorbent resin
Element, then through ethyl acetate purification process.
Invention further provides Radix Carpesii extract preparation preventing and treating ash arrhizus bacteria, Fulvia fulva, Fructus Lycopersici esculenti early
Application in epidemic disease bacterium, cucumber fusarium axysporum, target bacterium and/or P. capsici medicine.
Present invention also offers the preparation method of bactericidal microemulsion containing Radix Carpesii extract, in a kettle., first will
Radix Carpesii extract, insecticides adjuvant is dissolved in ethyl acetate solvent, under the conditions of 40 DEG C~50 DEG C, in the case of high-speed stirred
Gained solvent is added in distilled water, permanent stirring 1h, obtain Radix Carpesii bactericidal microemulsion.
The bactericidal microemulsion containing Radix Carpesii extract prepared according to the method described above.
Containing the bactericidal microemulsion of Radix Carpesii extract, include according to a point percentage by weight: Radix Carpesii extract 1~3 parts,
Solvent 1~2 parts, water 3~5 parts, insecticides adjuvant 1~2.
The present invention compares with antibacterial commonly used in the prior art, has a following features:
The material that reactive compound in the most environmentally safe Radix Carpesii antibacterial is naturally-occurring, derives from nature,
Long-term evolutionary process has defined its intrinsic participation energy and material cyclic metabolic pathway, thus use with environment in or
On plant, noresidue, will not cause biological concentration:
2. pair non-target organism is comparatively safe;
3. the mechanism of action of pair phytopathogen is different from conventional pesticide, uses rear pathogen to be difficult to it and produces drug resistance
Property, pathogen also there is is retarding action to the Drug resistance of conventional sterilization agent.
The Radix Carpesii antibacterial of the present invention is environmentally safe, and the reactive compound in Radix Carpesii antibacterial is naturally-occurring
Material, derives from nature, has defined its intrinsic participation energy and material cyclic metabolic pathway in long-term evolutionary process,
So being applied in environment or on plant, noresidue, biological concentration will not be caused;Comparatively safe to non-target organism;To plant
The mechanism of action of pathogen is different from conventional pesticide, uses rear pathogen to be difficult to it and develops immunity to drugs, to pathogen to routine
The Drug resistance of antibacterial also has retarding action.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is elaborated.
Embodiment 1: the preparation of Radix Carpesii antibacterial activity extract:
Take Radix Carpesii dry sample 100kg, the alcohol reflux adding 70% 2 times, add the ethanol of 800kg 70% for the first time, the
Secondary adds the ethanol of 600kg 70%, filters, united extraction liquid, and extracting solution reclaims ethanol and is concentrated into the dilute of relative density 1.05
Extractum, is extracted with ethyl acetate to obtain ethyl acetate phase by this dilute extractum, and ethyl acetate crosses D101 macroporous adsorbent resin mutually, first uses
This 30% ethanol elution, to colourless, is discarded by the ethanol elution of 30%, then the ethanol elution with 85% is the most colourless, collects this
85% ethanol elution, reclaims ethanol and is condensed into the thick paste of relative density 1.25, and vacuum drying obtains and carries containing Radix Carpesii is antibacterial
Take thing (based on lactone, mass fraction is not less than 60%).
Embodiment 2: the preparation of Radix Carpesii bactericidal microemulsion:
Antibacterial for Radix Carpesii refined extract 2 parts is joined in adjustment stirred autoclave, by insecticides adjuvant Termul 5030
1 part, by ethyl acetate 2 parts dissolving, high-speed stirred at a temperature of 40 DEG C~50 DEG C, adds deionized water 3 parts in whipping process, permanent
Temperature stirring 1h, obtains 40% Radix Carpesii bactericidal microemulsion.
Embodiment 3: Radix Carpesii microemulsion Determination of Antibacterial Activity
The PDA culture medium of Radix Carpesii microemulsion 0.02mL fusing embodiment 2 prepared is settled to 10mL, falls after mixing
Enter and culture dish is prepared as the flat board that carries disease germs.Cultivate 5-7 days for examination pathogen flat board in advance, cultivating with the card punch after sterilizing
Buying bacterium cake (a diameter of 4mm) on good bacterium colony, then bacterium cake tips upside down on mixed medicine flat board central authorities, process is repeated 3 times.Arrange
The aquesterilisa of amount is as comparison.The strain flat board connected is put into constant incubator (25 ± 1 DEG C) and is cultivated, by decussation method respectively
Measure the colony diameter of 72h, calculate suppression ratio as follows, the results are shown in Table 1.
Colony growth diameter=colony diameter-4
Table 1 Radix Carpesii microemulsion bacteriostatic activity
Note: in table, data are the meansigma methods repeated for three times, with significant difference (P between letter representation data different after column data
< 0.05, Duncan duncan's new multiple range method);"-" represents and is not detected by bacteriostatic activity.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described.The technology of the industry
Personnel, it should be appreciated that the present invention is not restricted to the described embodiments, simply illustrating this described in above-described embodiment and description
The principle of invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, and these become
Change and improvement both falls within scope of the claimed invention.Claimed scope by appending claims and
Equivalent defines.
Claims (8)
1. Radix Carpesii extract, it is characterised in that prepare after being extracted by the root of Radix Carpesii, stem, leaf and/or seed.
2. the preparation method of Radix Carpesii extract, it is characterised in that comprise the following steps: step one, by dry Radix Carpesii root,
Stem, leaf and/or seed are pulverized, and add 80%~the ethanol of 95% extracts, and collect filtrate, obtain Radix Carpesii crude extract;Step 2,
Radix Carpesii crude extract extracts through ethyl acetate, obtains Radix Carpesii antimicrobial extract.
The preparation method of Radix Carpesii extract the most according to claim 2, it is characterised in that described step one carries repeatedly
Taking and repeatedly no longer change to lixiviating solution color, merging filtrate obtains extracting solution, this extracting solution is condensed into dilute extractum, obtains Radix Carpesii thick
Extract.
The preparation method of Radix Carpesii extract the most according to claim 2, it is characterised in that Radix Carpesii in described step 2
Crude extract ester extract after ethyl acetate extracts sloughs pigment through macroporous adsorbent resin, then through ethyl acetate purification process.
5. Radix Carpesii extract preparation preventing and treating ash arrhizus bacteria, Fulvia fulva, tomato early blight bacterium, cucumber fusarium axysporum,
Application in target bacterium and/or P. capsici medicine.
6. contain the preparation method of the bactericidal microemulsion of Radix Carpesii extract, it is characterised in that in a kettle., first by Radix Carpesii
Extract, insecticides adjuvant is dissolved in ethyl acetate solvent, under the conditions of 40 DEG C~50 DEG C, by gained in the case of high-speed stirred
Solvent adds in distilled water, permanent stirring 1h, obtains Radix Carpesii bactericidal microemulsion.
7. contain the bactericidal microemulsion of Radix Carpesii extract, it is characterised in that prepare in accordance with the method for claim 6.
8. contain the bactericidal microemulsion of Radix Carpesii extract, it is characterised in that include according to a point percentage by weight: Radix Carpesii extracts
Thing 1~3 parts, solvent 1~2 parts, water 3~5 parts, insecticides adjuvant 1~2 parts.
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CN201610604491.XA CN106135298A (en) | 2016-07-28 | 2016-07-28 | Radix Carpesii extract and its preparation method and application |
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CN201610604491.XA CN106135298A (en) | 2016-07-28 | 2016-07-28 | Radix Carpesii extract and its preparation method and application |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106804629A (en) * | 2017-01-17 | 2017-06-09 | 长江大学 | To the extract and its bactericidal composition in section Chinese wax with bactericidal activity |
CN108794190A (en) * | 2018-07-26 | 2018-11-13 | 广西田阳县创新农业综合开发有限公司 | A kind of insect prevention fertilizer of mango and preparation method thereof |
CN108911845A (en) * | 2018-07-26 | 2018-11-30 | 广西田阳县创新农业综合开发有限公司 | A kind of mango Special slow release fertilizer and preparation method thereof |
CN111315234A (en) * | 2017-10-06 | 2020-06-19 | 嘉吉公司 | Easily soluble steviol glycoside compositions |
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CN101773532A (en) * | 2010-02-24 | 2010-07-14 | 周小江 | Carpesium abrotanoides total terpene lactones extract |
CN101856038A (en) * | 2009-10-19 | 2010-10-13 | 江苏省农业科学院 | Carpesium fruit extract for preventing and controlling crop insects |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106804629A (en) * | 2017-01-17 | 2017-06-09 | 长江大学 | To the extract and its bactericidal composition in section Chinese wax with bactericidal activity |
CN111315234A (en) * | 2017-10-06 | 2020-06-19 | 嘉吉公司 | Easily soluble steviol glycoside compositions |
CN111315234B (en) * | 2017-10-06 | 2024-01-02 | 嘉吉公司 | Easily soluble steviol glycoside composition |
CN108794190A (en) * | 2018-07-26 | 2018-11-13 | 广西田阳县创新农业综合开发有限公司 | A kind of insect prevention fertilizer of mango and preparation method thereof |
CN108911845A (en) * | 2018-07-26 | 2018-11-30 | 广西田阳县创新农业综合开发有限公司 | A kind of mango Special slow release fertilizer and preparation method thereof |
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Application publication date: 20161123 |