CN106115936A - A kind of method removing high concentration chrome wastewater pollutant - Google Patents

A kind of method removing high concentration chrome wastewater pollutant Download PDF

Info

Publication number
CN106115936A
CN106115936A CN201610581674.4A CN201610581674A CN106115936A CN 106115936 A CN106115936 A CN 106115936A CN 201610581674 A CN201610581674 A CN 201610581674A CN 106115936 A CN106115936 A CN 106115936A
Authority
CN
China
Prior art keywords
liquid
parts
solid
sewage
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610581674.4A
Other languages
Chinese (zh)
Other versions
CN106115936B (en
Inventor
李娜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rongsheng Chemical Co.,Ltd.
Original Assignee
李娜
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 李娜 filed Critical 李娜
Priority to CN201610581674.4A priority Critical patent/CN106115936B/en
Publication of CN106115936A publication Critical patent/CN106115936A/en
Application granted granted Critical
Publication of CN106115936B publication Critical patent/CN106115936B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00—Multistage treatment of water, waste water or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F2001/007—Processes including a sedimentation step
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/101—Sulfur compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/20—Heavy metals or heavy metal compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/20—Heavy metals or heavy metal compounds
    • C02F2101/22—Chromium or chromium compounds, e.g. chromates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/34—Organic compounds containing oxygen
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/40—Organic compounds containing sulfur
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/06—Controlling or monitoring parameters in water treatment pH
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed

Abstract

The present invention relates to a kind of method removing high concentration chrome wastewater pollutant, comprise the steps: first by sewage through solid-liquid separator, carry out solid-liquid separation, remove bulk solid particulate matter, liquid enters sedimentation tank subsequently, precipitate 12 hours, again liquid is removed solid floccules by circular hole drainage screen, through the liquid entrance biological reaction pool of circular hole filter screen filtration, regulation PH is 7, adds composite bacteria agent capable 10 grams by every cubic metre of liquid every time, add 1 every day, add continuously one week, finally stand 3 days, liquid is discharged.The method of the present invention can be removed effectively containing ammonia nitrogen sulfur phosphorus and the pollutant of chromium, and input cost is low, possesses preferable application prospect.

Description

A kind of method removing high concentration chrome wastewater pollutant
Technical field
The invention belongs to environmental technology field, be specifically related to a kind of method removing dirty water pollutant.
Background technology
Along with industrial development and the raising of people's living standard, trade effluent amount and city domestic sewage Amount surges the most with surprising rapidity, these sewage or or polluted river, river and the lake that the mankind depend on for existence, structure Become one of reason of prestige association environment for human survival.
Improving constantly environmental quality requirement to meet the public, nitrogen has been worked out the strictest discharge mark by country Standard, research and development are economical, denitrogenate treatment technology efficiently has become emphasis and the focus of Water Pollution Control Engineering area research.Though Many methods are so had can effectively to remove ammonia, as physical method has reverse osmosis, distillation, soil irrigation；Chemical method has ion to hand over Change method, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatments, catalytic pyrolysis；Biological method has nitrification and algae Class cultivates, but physical method treatment effect is the best, and than chemical method, biological method processes waste water and has the following advantages: 1) every kind The product that being both for property of chemical article is the strongest, is possible to when running into other chemical substances lose efficacy, and biological preparation is to dirt The removal of dye thing has light spectrality；2) chemical products can temporarily eliminate some harmful substance and cover stink, lacks and can not hinder The only generation of harmful substance；3), after using chemical products, water body has residual, secondary pollution may be caused.Biological preparation institute Containing natural microbial, without pathogenic bacterium and pathogen, these microorganisms are under the catalytic action of enzyme, with the organic nutrition in sewage Material is food, and after sewage is purified, these microbes gradually decrease with the reduction of pollutant, until withering away；4) Nontoxic, non-corrosiveness, easy to use, substantially need not interpolation equipment or engineering, save fund input.
Sewage mainly has sanitary sewage and industrial wastewater.Trade effluent comparison of ingredients is complicated, the most substantial amounts of artificial conjunction Becoming compound to enter environment, this kind of material is mainly ammonia nitrogen class, sulfide and phosphorus-containing compound, owing to these materials itself are tied The complexity of structure, can not be decomposed by the microorganisms utilization at short notice, and traditional method of wastewater treatment activated sludge culture is tamed and dociled These pollutant can not be removed by microorganism effectively that change, and these materials accumulate the most in the environment, relies to us Cause the biggest pollution with the ecological environment of existence, bring the biggest harm to the physical and mental health of the mankind.Quite a few industry of China Contaminating enterprises would rather be punished also to be unwilling to invest and administer waste water, even if there have sewage-treatment plant to run to be the most abnormal.Therefore, open Sending out a kind of, construction investment is few, operating cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
Summary of the invention
In order to overcome the deficiencies in the prior art, effectively simply remove the ammonia nitrogen sulfur phosphorus in sewage and heavy metal etc. and pollute Thing, the invention provides a kind of method removing dirty water pollutant, and its technical scheme realizes by the following method:
A kind of method removing high concentration chrome wastewater pollutant, it comprises the steps:
The preparation of composite bacteria agent capable: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stirs, then stand 6 Hour, cold drying at the most rearmounted 4 DEG C, dried water content control, 6%, to obtain final product；Described carrier by bamboo charcoal, chitosan and Kieselguhr is mixed to get according to the mass ratio of 2:2:1, the particle diameter of bamboo charcoal preferably 10 mesh；
Sewage disinfection treatment: first by sewage through solid-liquid separator, carries out solid-liquid separation, removes bulk solid particulate matter Matter, liquid enters sedimentation tank subsequently, precipitates 12 hours, then by circular hole drainage screen, liquid is removed solid floccules, circular hole mistake The Circularhole diameter of filter screen is 0.1mm, biological oxidation: through the liquid entrance biological reaction pool of circular hole drainage screen, regulation pH is 7, presses Every cubic metre of liquid adds composite bacteria agent capable 10 grams every time, adds every day 1 time, adds continuously one week, finally stands 3 days, by liquid Discharge.
Preferably, described composite bacteria agent capable is mixed by the raw material bacterium of following weight portion:
Rhodococcus fascians 10 parts, thiobacillus denitrificans 9 parts, Pseudomonas stutzeri 7 parts, Sphingomonas 6 parts, Bacillus pumilus 5 parts, Phanerochaete chrysosporium 2 parts；The concentration of above-mentioned each raw material bacterium all controls in (1-2) × 108Individual/ml.Above-mentioned strain is permissible It is the conventional bacterial strain of prior art,
Preferably,
Described Rhodococcus fascians is Rhodococcus fascians (Rhodococcusrhodochrous) ATCC15906；
Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC25259；
Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M209107；
Described Sphingomonas is Sphingomonas (Sphingomonas sp.) CGMCC N0.4589；
Described Bacillus pumilus is Bacillus pumilus (Bacillus pumilus) ATCC27142；
Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC24725。
Bacterium of the present invention all can be from business such as CGMCC, CCTCC and American Type Culture collection warehousing (ATCC) Approach is commercially available.
Preferably, described thiobacillus denitrificans is the encoding gene having proceeded to coding any one protein variant following ATCC25259 bacterial strain, described protein variant for relative to albumen original amino acid suddenly change the most as follows 30G/E, 59L/S、63L/T、92F/L、121E/G、189R/V、240E/P、253Q/S、300C/P、309G/S、321A/P、339S/I、 359L/H, 363V/G, albumen original amino acid sees GenBank:NP_745112.1.
Present invention simultaneously provides a kind of can the transgenic strain of efficient absorption chromium, this bacterial strain is by by aminoacid sequence such as Sequence shown in Genbank:NP_745112.1 imports to realize its purpose in thiobacillus denitrificans.
And described aminoacid sequence can have a sudden change in following no site, 30G/E, 59L/S, 63L/T, 92F/L、121E/G、189R/V、240E/P、253Q/S、300C/P、309G/S、321A/P、339S/I、359L/H、363V/G。 (30G/E represents that the G aminoacid original series the 30th replaces with E aminoacid).Aminoacid sequence after these sudden changes is not grudged The bacterial strain having imported the aminoacid sequence after this sudden change has similar effect equally.And applicant is by substantial amounts of experiment card Real, not all replacement all has similar effect, has the thiobacillus denitrificans after substantial amounts of replacement and other bacterial strain one Play the effect without preferable sewage disposal.
Utilize DNAMAN software design primer, be separately added into restriction enzyme site, complete according to aminoacid sequence NP_745112.1 Nucleotide sequence PDA gene described in gene chemical synthesis, obtains purpose fragment by carrying out amplification, and PCR amplification obtains genes of interest Corresponding mutational site (is incorporated in gene order by multiplex PCR simultaneously, thus obtains different mutants base by PDA Cause), carry out double digestion PCR primer with restricted enzyme, be connected with the cloning vector PWB980 also passing through double digestion, The recombinant plasmid transformed being proved to be successful is entered in described thiobacillus denitrificans bacterium, it is thus achieved that the genetic engineering bacterium of degraded chromium.
The amplification culture of each strain of the present invention is the cellar culture mode of this area, it is also possible to described in reference literature Training method obtain.
What the present invention obtained mainly has the beneficial effect that: the present invention uses pure microbial inoculum to process sewage, has effectively purified dirt Water, and dephosphorization, denitrogenation remove simultaneously, and the heavy metal chromium that simultaneously can remove sewage Central China on this basis solves tradition work In skill, dephosphorization and denitrification effect are difficult to take into account the contradiction of heavy metals removal simultaneously, have preferable application prospect.
Detailed description of the invention
Embodiment 1
A kind of method removing dirty water pollutant, it comprises the steps:
The preparation of composite bacteria agent capable: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stirs, then stand 6 Hour, cold drying at the most rearmounted 4 DEG C, dried water content control, 6%, to obtain final product；Carrier is by bamboo charcoal, chitosan and diatom Soil is mixed to get according to the mass ratio of 2:2:1；Above-mentioned mixed bacteria liquid is mixed by the raw material bacterium of following weight portion: Rhodococcus fascians 10 parts, thiobacillus denitrificans 9 parts, Pseudomonas stutzeri 7 parts, Sphingomonas 6 parts, Bacillus pumilus 5 parts, yellow archespore hair is put down Lead fungi 2 parts；The concentration of above-mentioned each raw material bacterium all controls at I X I08/ml.
Sewage disinfection treatment: being first 300mg/L by sewage NH3-N, sulfide 80mg/L, is 70mg/L containing phosphor contaminant, Cadmium content is 35mg/ml, through solid-liquid separator, carries out solid-liquid separation, removes bulk solid particulate matter, and liquid enters subsequently Sedimentation tank, precipitates 12 hours, then by circular hole drainage screen, liquid is removed solid floccules, and the Circularhole diameter of circular hole drainage screen is 0.1mm；
Biological oxidation: through the liquid entrance biological reaction pool of circular hole drainage screen, regulation pH is 7.0, by every cubic metre of liquid Adding composite bacteria agent capable 10 grams, every day adds I time every time, adds continuously one week, finally stands 3 days, is discharged by liquid.After testing, The content of ammonia nitrogen in sewage is respectively 12.5mg/L, sulfide 4.5mg/L, is 2.5mg/L containing phosphor contaminant, and clearance all reaches More than 95%；And chromium content is 33mg/mL, removal effect is the most notable.
Embodiment 2
Utilize DNAMAN software design primer, be separately added into restriction enzyme site, complete according to aminoacid sequence NP_745112.1 Nucleotide sequence GP gene described in gene chemical synthesis, obtains purpose fragment by carrying out amplification, and PCR amplification obtains genes of interest GP (simultaneously by multiplex PCR by corresponding mutational site 30G/E, 59L/S, 63L/T, 92F/L, 121E/G, 189R/V, 240E/P, 253Q/S, 300C/P, 309G/S, 321A/P, 339S/I, 359L/H, 363V/G are incorporated in gene order, thus obtain not Same mutant gene), double digestion PCR primer, it is connected with the cloning vector PWB980 also passing through double digestion, checking is become The recombinant plasmid transformed of merit enters in described thiobacillus denitrificans ATCC25259, it is thus achieved that the genetic engineering bacterium of degraded chromium.
The wastewater treatment efficiency checking of embodiment 3 thiobacillus denitrificans genetic engineering bacterium and remaining several microbial inoculum
According to the method for embodiment 1, carrying out corresponding sewage disposal experiment, its sewage belongs to same with the sewage of embodiment 1 One batch, has the pollutant levels of same concentrations.Wherein each component composition of microbial inoculum is identical with embodiment 1.
The genetic engineering bacterium in the different mutational site being respectively adopted embodiment 2 preparation is removed the reaction of chromium, passes through Experiment find, 30G/E, 59L/S, 63L/T, 92F/L, 121E/G, 189R/V, 240E/P, 253Q/S, 300C/P, 309G/S, These several mutant bacterias of 321A/P, 339S/I, 359L/H, 363V/G all have and have the effect being remarkably reinforced relative to original bacteria. Being not more than total processing time 4 days, its result is as follows: sewage is with a collection of sewage, thus guaranteed conditions is consistent.
Each pollutant levels of sewage: NH3-N is 300mg/L, sulfide 80mg/L, is 70mg/L containing phosphor contaminant, chromium Content is 35mg/ml.
As can be seen from the above results, the bacterium that the thiobacillus denitrificans using genetic engineering to build is constituted with other several bacterial strains Agent, in addition to having the effect of original preferable removal ammonia nitrogen, sulfide and phosphorus, also has the effect preferably removing chromium, And in terms of removing ammonia nitrogen, sulfide and phosphorus, also there is between engineered thiobacillus denitrificans and other several bacterial strains increasing Strong synergism.

Claims (4)

1. the method removing high concentration chrome wastewater pollutant, its comprise the steps: first by sewage through solid-liquid separator, enter Row solid-liquid separation, removes bulk solid particulate matter, and liquid enters sedimentation tank subsequently, precipitates 12 hours, then by liquid by circle Hole drainage screen removes solid floccules, and through the liquid entrance biological reaction pool of circular hole filter screen filtration, regulation PH is 7, by often standing Side's rice liquid adds composite bacteria agent capable 10 grams every time, adds every day 1 time, adds continuously one week, finally stands 3 days, is discharged by liquid.
2. the method for claim 1, it is characterised in that: described composite bacteria agent capable is by the raw material bacterium mixing of following weight portion Become: Rhodococcus fascians 10 parts, thiobacillus denitrificans 9 parts, Pseudomonas stutzeri 7 parts, Sphingomonas 6 parts, Bacillus pumilus 5 parts, Phanerochaete chrysosporium 2 parts；The concentration of above-mentioned each raw material bacterium all controls in (1-2) × 108Individual/ml.
3. method as claimed in claim 2, it is characterised in that: described thiobacillus denitrificans is for having proceeded to coding any one egg following The ATCC25259 bacterial strain of the encoding gene of leucismus body, described protein variant for relative to albumen original amino acid carry out as Lower sudden change 121E/G, albumen original amino acid sees GenBank:NP_745112.1.
4. a protein variant, it is characterised in that: described protein variant is for carry out as follows relative to albumen original amino acid Sudden change 121E/G, albumen original amino acid sees GenBank:NP_745112.1.
CN201610581674.4A 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants Active CN106115936B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610581674.4A CN106115936B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201510441588.9A CN104973738B (en) 2015-07-26 2015-07-26 Method for removing pollutants from chromium-containing sewage
CN201610581674.4A CN106115936B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201510441588.9A Division CN104973738B (en) 2015-07-26 2015-07-26 Method for removing pollutants from chromium-containing sewage

Publications (2)

Publication Number Publication Date
CN106115936A true CN106115936A (en) 2016-11-16
CN106115936B CN106115936B (en) 2021-10-15

Family

ID=54270768

Family Applications (13)

Application Number Title Priority Date Filing Date
CN201610581675.9A Active CN106007238B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610582797.XA Expired - Fee Related CN105967464B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610582799.9A Active CN106007242B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610582800.8A Active CN106007243B (en) 2015-07-26 2015-07-26 A method of removal high concentration chrome wastewater pollutant
CN201510441588.9A Expired - Fee Related CN104973738B (en) 2015-07-26 2015-07-26 Method for removing pollutants from chromium-containing sewage
CN201610582801.2A Expired - Fee Related CN106007244B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581765.8A Active CN105948427B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581672.5A Active CN106007237B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581673.XA Active CN105967461B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610582798.4A Active CN105948279B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581674.4A Active CN106115936B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581764.3A Active CN106007240B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581781.7A Active CN105967462B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants

Family Applications Before (10)

Application Number Title Priority Date Filing Date
CN201610581675.9A Active CN106007238B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610582797.XA Expired - Fee Related CN105967464B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610582799.9A Active CN106007242B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610582800.8A Active CN106007243B (en) 2015-07-26 2015-07-26 A method of removal high concentration chrome wastewater pollutant
CN201510441588.9A Expired - Fee Related CN104973738B (en) 2015-07-26 2015-07-26 Method for removing pollutants from chromium-containing sewage
CN201610582801.2A Expired - Fee Related CN106007244B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581765.8A Active CN105948427B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581672.5A Active CN106007237B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581673.XA Active CN105967461B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610582798.4A Active CN105948279B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN201610581764.3A Active CN106007240B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants
CN201610581781.7A Active CN105967462B (en) 2015-07-26 2015-07-26 Method for removing chromium-containing sewage pollutants

Country Status (1)

Country Link
CN (13) CN106007238B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105523642A (en) * 2015-12-21 2016-04-27 天津欧盼科技开发有限公司 Heavy metal polluted water body restoration agent
CN106746350A (en) * 2017-01-15 2017-05-31 海宁蒙努皮革制品有限公司 Improved leather waste water retracting device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5804435A (en) * 1994-02-02 1998-09-08 Tonen Corporation Method for obtaining organic solvent-resistant microorganisms and organic solvent-resistant microorganisms obtainable by the method
CN101857846A (en) * 2010-04-20 2010-10-13 深圳清华大学研究院 Rhodococcus erythropolis and microbial inoculum and application thereof
CN103449680A (en) * 2013-09-13 2013-12-18 李丽萍 Method for removing pollutants in sewage

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1047155C (en) * 1993-06-11 1999-12-08 中国科学院成都生物研究所 New technology for treating electroplating waste water with micro-organism
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
BRPI0600263B1 (en) * 2006-01-18 2021-04-13 Fundação De Amparo À Pesquisa Do Estado de São Paulo USE OF MACROLAMPIS SP LUCIFERASE AS A DUAL GENE REPORTER IN SIMULTANEOUS BIOSENSORS OF GENE EXPRESSION AND INTRACELLULAR PH VARIATIONS, PHOSPHATE CONCENTRATIONS AND DIVALENT HEAVY METAL CATIONS
KR100755746B1 (en) * 2006-05-22 2007-09-06 한국과학기술원 Method for preparing quantum dot using metal binding protein and recombinant microorganisms therefor
US20120080374A1 (en) * 2010-10-04 2012-04-05 Pacific Advanced Civil Engineering, Inc. Ozone and anaerobic biological pretreatment for a desalination process
WO2013077780A1 (en) * 2011-11-22 2013-05-30 Hans Giertz Method and apparatus for purifying aqueous solutions by shielding or subjecting chemical substances to low frequency electromagnetic radiation
CN103374062B (en) * 2012-04-23 2015-09-30 中国科学院上海生命科学研究院 OsVIT1 and OsVIT2 gene and improve the application of iron Zn content in rice paddy seed
CN104355497B (en) * 2014-11-13 2016-04-06 国网山东平邑县供电公司 A kind of method processing electroplating wastewater

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5804435A (en) * 1994-02-02 1998-09-08 Tonen Corporation Method for obtaining organic solvent-resistant microorganisms and organic solvent-resistant microorganisms obtainable by the method
CN101857846A (en) * 2010-04-20 2010-10-13 深圳清华大学研究院 Rhodococcus erythropolis and microbial inoculum and application thereof
CN103449680A (en) * 2013-09-13 2013-12-18 李丽萍 Method for removing pollutants in sewage

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张迎明 等: "《镍钴转运酶NiCoT基因的克隆表达及基因工程菌对镍离子的富集》", 《环境科学》 *

Also Published As

Publication number Publication date
CN104973738B (en) 2017-01-18
CN106007244B (en) 2021-02-05
CN106007244A (en) 2016-10-12
CN106007243B (en) 2019-05-10
CN105948427B (en) 2021-10-29
CN105948427A (en) 2016-09-21
CN105948279B (en) 2021-04-09
CN104973738A (en) 2015-10-14
CN105948279A (en) 2016-09-21
CN105967464B (en) 2021-04-27
CN106115936B (en) 2021-10-15
CN106007238B (en) 2021-10-29
CN105967462A (en) 2016-09-28
CN106007237A (en) 2016-10-12
CN106007237B (en) 2021-10-29
CN106007243A (en) 2016-10-12
CN106007240A (en) 2016-10-12
CN105967464A (en) 2016-09-28
CN105967461A (en) 2016-09-28
CN105967462B (en) 2021-06-04
CN105967461B (en) 2021-09-24
CN106007242A (en) 2016-10-12
CN106007242B (en) 2021-10-26
CN106007238A (en) 2016-10-12
CN106007240B (en) 2021-04-27

Similar Documents

Publication Publication Date Title
CN105036471B (en) A kind of method for removing leaded wastewater pollutants
CN105130100B (en) It is a kind of to remove the method containing cadmium wastewater pollutants
CN104973738B (en) Method for removing pollutants from chromium-containing sewage
CN106430865B (en) A method of removing mercurous wastewater pollutants
CN106587506B (en) A method of removal wastewater pollutants containing arsenic

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20210913

Address after: 062550 north of 50m Road East of forensic hospital, Jinbao Road, Renqiu City, Cangzhou City, Hebei Province

Applicant after: Rongsheng Chemical Co.,Ltd.

Address before: 100193 China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing

Applicant before: Li Na

GR01 Patent grant
GR01 Patent grant