CN105967462A - Method for removing chrome-contained wastewater pollutants - Google Patents
Method for removing chrome-contained wastewater pollutants Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000003344 environmental pollutant Substances 0.000 title claims abstract description 12
- 231100000719 pollutant Toxicity 0.000 title claims abstract description 12
- 239000002351 wastewater Substances 0.000 title claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 31
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000007787 solid Substances 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 5
- 238000004062 sedimentation Methods 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 4
- 239000010865 sewage Substances 0.000 claims description 25
- 241000894006 Bacteria Species 0.000 claims description 23
- 108090000623 proteins and genes Proteins 0.000 claims description 15
- 241001509286 Thiobacillus denitrificans Species 0.000 claims description 14
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 150000001413 amino acids Chemical class 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 239000002131 composite material Substances 0.000 claims description 7
- 241000194103 Bacillus pumilus Species 0.000 claims description 6
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims description 6
- 241000589614 Pseudomonas stutzeri Species 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 241000187694 Rhodococcus fascians Species 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 5
- 241000736131 Sphingomonas Species 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 239000013618 particulate matter Substances 0.000 claims description 2
- 238000009288 screen filtration Methods 0.000 claims 1
- CPJBEAMIONWDDK-UHFFFAOYSA-N [N].N.[P].[S] Chemical compound [N].N.[P].[S] CPJBEAMIONWDDK-UHFFFAOYSA-N 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 239000002054 inoculum Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
- 229910052804 chromium Inorganic materials 0.000 description 8
- 239000011651 chromium Substances 0.000 description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 6
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 6
- 238000010353 genetic engineering Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 125000003275 alpha amino acid group Chemical group 0.000 description 4
- 229910021529 ammonia Inorganic materials 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000002068 microbial inoculum Substances 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 3
- 235000017491 Bambusa tulda Nutrition 0.000 description 3
- 241001330002 Bambuseae Species 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 239000011425 bamboo Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
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- 239000000356 contaminant Substances 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
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- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229920001661 Chitosan Polymers 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 239000013599 cloning vector Substances 0.000 description 2
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- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 101150117028 GP gene Proteins 0.000 description 1
- 241000187693 Rhodococcus rhodochrous Species 0.000 description 1
- 241001135759 Sphingomonas sp. Species 0.000 description 1
- 241001136391 Thiobacillus denitrificans ATCC 25259 Species 0.000 description 1
- CVTZKFWZDBJAHE-UHFFFAOYSA-N [N].N Chemical class [N].N CVTZKFWZDBJAHE-UHFFFAOYSA-N 0.000 description 1
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- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
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- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
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- 238000012545 processing Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
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- 238000012827 research and development Methods 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
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- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/40—Organic compounds containing sulfur
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
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- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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Abstract
The invention relates to a method for removing chrome-contained wastewater pollutants. The method comprises the following steps that firstly, wastewater is subjected to solid-liquid separation through a solid-liquid separator, large solid particulate matters are removed, then, liquid enters a sedimentation basin for sedimentation for 12 h, then the liquid passes through a circular hole filter net so that solid floccules can be removed, the liquid filtered through the circular hole filter net enters a biological reaction basin, PH is adjusted to 7, 10 g of complex microbial inoculants are added into per cubic meter of liquid every time, addition is performed one time per day and continues to be performed for one week, finally standing is performed for 3 days, and liquid is drained. According to the method, ammonia nitrogen sulfur phosphorus and chrome contained pollutants can be effectively removed, the input cost is low, and the good application prospect is achieved.
Description
Technical field
The invention belongs to environmental technology field, be specifically related to a kind of method removing dirty water pollutant.
Background technology
Along with industrial development and the raising of people's living standard, trade effluent amount and urban life
Sewage quantity surges the most with surprising rapidity, these sewage or or polluted river that the mankind depend on for existence,
River and lake, constituted one of reason of prestige association environment for human survival.
Improving constantly environmental quality requirement to meet the public, nitrogen has been worked out the strictest row by country
Putting standard, research and development are economical, denitrogenate treatment technology efficiently has become Water Pollution Control Engineering area research
Emphasis and focus.Although there being many methods can effectively remove ammonia, as physical method have reverse osmosis, distillation,
Soil irrigation；Chemical method has ion exchange, ammonia aeration, chemical precipitation method, breakpoint chlorination, electrodialysis, electricity
Chemical treatment, catalytic pyrolysis；Biological method has nitrification and algal culture, but physical method treatment effect is the best,
Than chemical method, biological method processes waste water and has the following advantages: 1) every kind of being both for property of chemical article is the strongest
Product, is possible to when running into other chemical substances lose efficacy, and biological preparation has light to the removal of pollutant
Spectrality；2) chemical products can temporarily eliminate some harmful substance and cover stink, lacks and can not stop nuisance
The generation of matter；3), after using chemical products, water body has residual, secondary pollution may be caused.Biological preparation
Contained natural microbial, without pathogenic bacterium and pathogen, these microorganisms are under the catalytic action of enzyme, with sewage
In organic nutrient substance be food, after sewage is purified, these microbes with pollutant reduction and
Gradually decrease, until withering away；4) nontoxic, non-corrosiveness, easy to use, substantially need not interpolation equipment or
Engineering, saves fund input.
Sewage mainly has sanitary sewage and industrial wastewater.Trade effluent comparison of ingredients is complicated, the most substantial amounts of people
Work synthesis compound enters environment, and this kind of material is mainly ammonia nitrogen class, sulfide and phosphorus-containing compound, due to
The complexity of these these body structures of material, can not be decomposed by the microorganisms utilization, at traditional waste water at short notice
These pollutant can not be removed by the microorganism of reason method activated sludge culture effectively, these
Material accumulates the most in the environment, and the ecological environment depended on for existence to us causes the biggest pollution, to the body of the mankind
Heart health brings the biggest harm.Quite a few industrial pollution enterprise of China would rather be punished also to be unwilling to invest and administer
Waste water, even if there have sewage-treatment plant to run to be the most abnormal.Therefore, exploitation one construction investment is few, operation
Low cost, good treatment efficiency sewage disposal technology extremely urgent.
Summary of the invention
In order to overcome the deficiencies in the prior art, effectively simply remove the ammonia nitrogen sulfur phosphorus in sewage and heavy metal etc.
Pollutant, the invention provides a kind of method removing dirty water pollutant, and its technical scheme is by such as lower section
Method realizes:
A kind of method removing high concentration chrome wastewater pollutant, it comprises the steps:
The preparation of composite bacteria agent capable: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stirs, so
Rear standing 6 hours, cold drying at the most rearmounted 4 DEG C, dried water content control, 6%, to obtain final product；Described
Carrier is mixed to get according to the mass ratio of 2:2:1 by bamboo charcoal, chitosan and kieselguhr, and the particle diameter of bamboo charcoal is excellent
Select 10 mesh；
Sewage disinfection treatment: first by sewage through solid-liquid separator, carries out solid-liquid separation, removes bulk solid
Grain material, liquid enters sedimentation tank subsequently, precipitates 12 hours, then is removed solid by circular hole drainage screen by liquid
Body flocculate, the Circularhole diameter of circular hole drainage screen is 0.1mm, biological oxidation: through the liquid entrance of circular hole drainage screen
Biological reaction pool, regulation pH is 7, adds composite bacteria agent capable 10 grams by every cubic metre of liquid every time, and every day adds
1 time, add continuously one week, finally stand 3 days, liquid is discharged.
Preferably, described composite bacteria agent capable is mixed by the raw material bacterium of following weight portion:
Rhodococcus fascians 10 parts, thiobacillus denitrificans 9 parts, Pseudomonas stutzeri 7 parts, Sphingomonas 6 parts,
Bacillus pumilus 5 parts, Phanerochaete chrysosporium 2 parts；The concentration of above-mentioned each raw material bacterium all control (1-2) ×
108Individual/ml.Above-mentioned strain can be the conventional bacterial strain of prior art,
Preferably,
Described Rhodococcus fascians is Rhodococcus fascians (Rhodococcusrhodochrous) ATCC15906；
Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC25259；
Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC
NO:M209107；
Described Sphingomonas is Sphingomonas (Sphingomonas sp.) CGMCC N0.4589；
Described Bacillus pumilus is Bacillus pumilus (Bacillus pumilus) ATCC27142；
Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete
chrysosporium)ATCC24725。
Bacterium of the present invention all can be from CGMCC, CCTCC and American Type Culture collection warehousing (ATCC)
It is commercially available etc. commercial sources.
Preferably, described thiobacillus denitrificans is the encoding gene having proceeded to coding any one protein variant following
ATCC25259 bacterial strain, described protein variant is for suddenly change the most as follows relative to albumen original amino acid
30G/E、59L/S、63L/T、92F/L、121E/G、189R/V、240E/P、253Q/S、300C/P、309G/S、
321A/P, 339S/I, 359L/H, 363V/G, albumen original amino acid sees GenBank:
NP_745112.1。
Present invention simultaneously provides a kind of can the transgenic strain of efficient absorption chromium, this bacterial strain is by by aminoacid sequence
Row sequence as shown in Genbank:NP_745112.1 imports to realize its purpose in thiobacillus denitrificans.
And described aminoacid sequence can have a sudden change in following no site, 30G/E, 59L/S,
63L/T、92F/L、121E/G、189R/V、240E/P、253Q/S、300C/P、309G/S、321A/P、
339S/I、359L/H、363V/G.(30G/E represents that the G aminoacid original series the 30th replaces with E ammonia
Base acid).The bacterial strain that aminoacid sequence after these sudden changes is not willing the aminoacid sequence after having imported this sudden change is same
There is similar effect.And applicant by substantial amounts of it is experimentally confirmed that not all replacement all has similar
Effect, the thiobacillus denitrificans after having substantial amounts of replacement does not have at preferable sewage together with other bacterial strain
The effect of reason.
Utilize DNAMAN software design primer, be separately added into restriction enzyme site, according to aminoacid sequence
Nucleotide sequence PDA gene described in the synthesis of NP_745112.1 full genome, obtains purpose sheet by carrying out amplification
Section, PCR amplification obtains genes of interest PDA and (by multiplex PCR, corresponding mutational site is incorporated into gene simultaneously
In sequence, thus obtain different mutants gene), carry out double digestion PCR primer with restricted enzyme,
It is connected with the cloning vector PWB980 also passing through double digestion, the recombinant plasmid transformed being proved to be successful is entered
In described thiobacillus denitrificans bacterium, it is thus achieved that the genetic engineering bacterium of degraded chromium.
The amplification culture of each strain of the present invention is the cellar culture mode of this area, it is also possible in reference literature
The training method recorded obtains.
What the present invention obtained mainly has the beneficial effect that: the present invention uses pure microbial inoculum to process sewage, effectively purifies
Sewage, and dephosphorization, denitrogenation remove simultaneously, can remove the heavy metal in sewage Central China on this basis simultaneously
Chromium solves dephosphorization and denitrification effect in traditional handicraft and is difficult to take into account the contradiction of heavy metals removal simultaneously, has preferably
Application prospect.
Detailed description of the invention
Embodiment 1
A kind of method removing dirty water pollutant, it comprises the steps:
The preparation of composite bacteria agent capable: mixed bacteria liquid and carrier are mixed according to the weight ratio of 1:1, stirs, then
Standing 6 hours, cold drying at the most rearmounted 4 DEG C, dried water content control, 6%, to obtain final product；Carrier is by bamboo
Charcoal, chitosan and kieselguhr are mixed to get according to the mass ratio of 2:2:1；Above-mentioned mixed bacteria liquid is by weighing as follows
The raw material bacterium of amount part mixes: Rhodococcus fascians 10 parts, thiobacillus denitrificans 9 parts, Pseudomonas stutzeri 7 parts, sheath ammonia
Alcohol sporangium 6 parts, Bacillus pumilus 5 parts, Phanerochaete chrysosporium 2 parts；The concentration of above-mentioned each raw material bacterium is equal
Control at I X I08/ml.
Sewage disinfection treatment: being first 300mg/L by sewage NH3-N, sulfide 80mg/L, containing phosphor contaminant
For 70mg/L, cadmium content is 35mg/ml, through solid-liquid separator, carries out solid-liquid separation, removes bulk solid
Body particulate matter, liquid enters sedimentation tank subsequently, precipitates 12 hours, then is removed by circular hole drainage screen by liquid
Solid floccules, the Circularhole diameter of circular hole drainage screen is 0.1mm；
Biological oxidation: through the liquid entrance biological reaction pool of circular hole drainage screen, regulation pH is 7.0, by every cubic metre
Liquid adds composite bacteria agent capable 10 grams every time, and every day adds I time, adds continuously one week, finally stands 3 days, by liquid
Body is discharged.After testing, the content of ammonia nitrogen in sewage is respectively 12.5mg/L, sulfide 4.5mg/L, contains
Phosphor contaminant is 2.5mg/L, and clearance all reaches more than 95%；And chromium content is 33mg/mL, removal effect
The most notable.
Embodiment 2
Utilize DNAMAN software design primer, be separately added into restriction enzyme site, according to aminoacid sequence
Nucleotide sequence GP gene described in the synthesis of NP_745112.1 full genome, obtains purpose fragment by carrying out amplification,
PCR amplification obtain genes of interest GP (simultaneously by multiplex PCR by corresponding mutational site 30G/E, 59L/S,
63L/T、92F/L、121E/G、189R/V、240E/P、253Q/S、300C/P、309G/S、321A/P、
339S/I, 359L/H, 363V/G are incorporated in gene order, thus obtain different mutants gene), double
Enzyme action PCR primer, is connected with the cloning vector PWB980 also passing through double digestion, the weight that will be proved to be successful
Group Plastid transformation enters in described thiobacillus denitrificans ATCC25259, it is thus achieved that the genetic engineering bacterium of degraded chromium.
The wastewater treatment efficiency checking of embodiment 3 thiobacillus denitrificans genetic engineering bacterium and remaining several microbial inoculum
According to the method for embodiment 1, carrying out corresponding sewage disposal experiment, its sewage belongs to the sewage of embodiment 1
In same batch, there are the pollutant levels of same concentrations.Wherein each component of microbial inoculum forms and embodiment 1
Identical.
The genetic engineering bacterium in the different mutational site being respectively adopted embodiment 2 preparation is removed the reaction of chromium,
Be found through experiments, 30G/E, 59L/S, 63L/T, 92F/L, 121E/G, 189R/V, 240E/P, 253Q/S,
These several mutant bacterias of 300C/P, 309G/S, 321A/P, 339S/I, 359L/H, 363V/G all have relative to
Original bacteria has the effect being remarkably reinforced.Being not more than total processing time 4 days, its result is as follows: sewage is same
Criticize sewage, thus guaranteed conditions is consistent.
Each pollutant levels of sewage: NH3-N is 300mg/L, and sulfide 80mg/L, containing phosphor contaminant
For 70mg/L, chromium content is 35mg/ml.
As can be seen from the above results, the thiobacillus denitrificans using genetic engineering to build is constituted with other several bacterial strains
Microbial inoculum in addition to there is the effect of original preferable removal ammonia nitrogen, sulfide and phosphorus, also have preferably
Remove the effect of chromium, and in terms of removing ammonia nitrogen, sulfide and phosphorus engineered thiobacillus denitrificans and its
Also there is between its several bacterial strains the synergism of enhancing.
Claims (4)
1. the method removing high concentration chrome wastewater pollutant, its comprise the steps: first by sewage through solid-liquid separator, carry out solid-liquid separation, remove bulk solid particulate matter, liquid enters sedimentation tank subsequently, precipitate 12 hours, again liquid is removed solid floccules by circular hole drainage screen, through the liquid entrance biological reaction pool of circular hole filter screen filtration, regulation PH is 7, adds composite bacteria agent capable 10 grams by every cubic metre of liquid every time, add 1 every day, add continuously one week, finally stand 3 days, liquid is discharged.
2. the method for claim 1, it is characterised in that: described composite bacteria agent capable is mixed by the raw material bacterium of following weight portion: Rhodococcus fascians 10 parts, thiobacillus denitrificans 9 parts, Pseudomonas stutzeri 7 parts, Sphingomonas 6 parts, Bacillus pumilus 5 parts, Phanerochaete chrysosporium 2 parts；The concentration of above-mentioned each raw material bacterium all controls in (1-2) × 108Individual/ml.
3. method as claimed in claim 2, it is characterized in that: described thiobacillus denitrificans is the ATCC25259 bacterial strain of the encoding gene having proceeded to coding any one protein variant following, described protein variant is for suddenling change as follows 300C/P relative to albumen original amino acid, and albumen original amino acid sees GenBank:NP_745112.1.
4. a protein variant, it is characterised in that: described protein variant is for suddenling change as follows 300C/P relative to albumen original amino acid, and albumen original amino acid sees GenBank:NP_745112.1.
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| CN201610582797.XA Expired - Fee Related CN105967464B (en) | 2015-07-26 | 2015-07-26 | Method for removing chromium-containing sewage pollutants |
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| CN201610582799.9A Active CN106007242B (en) | 2015-07-26 | 2015-07-26 | Method for removing chromium-containing sewage pollutants |
| CN201610581781.7A Active CN105967462B (en) | 2015-07-26 | 2015-07-26 | Method for removing chromium-containing sewage pollutants |
| CN201610582800.8A Active CN106007243B (en) | 2015-07-26 | 2015-07-26 | A method of removal high concentration chrome wastewater pollutant |
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| CN105967462B (en) | 2021-06-04 |
| CN105948427B (en) | 2021-10-29 |
| CN105967464A (en) | 2016-09-28 |
| CN106007237B (en) | 2021-10-29 |
| CN106007237A (en) | 2016-10-12 |
| CN106007243B (en) | 2019-05-10 |
| CN106007242A (en) | 2016-10-12 |
| CN106007238A (en) | 2016-10-12 |
| CN106115936B (en) | 2021-10-15 |
| CN105967461A (en) | 2016-09-28 |
| CN106007243A (en) | 2016-10-12 |
| CN106007244B (en) | 2021-02-05 |
| CN106115936A (en) | 2016-11-16 |
| CN104973738A (en) | 2015-10-14 |
| CN105948279A (en) | 2016-09-21 |
| CN105948279B (en) | 2021-04-09 |
| CN105948427A (en) | 2016-09-21 |
| CN104973738B (en) | 2017-01-18 |
| CN105967461B (en) | 2021-09-24 |
| CN105967464B (en) | 2021-04-27 |
| CN106007240A (en) | 2016-10-12 |
| CN106007238B (en) | 2021-10-29 |
| CN106007244A (en) | 2016-10-12 |
| CN106007242B (en) | 2021-10-26 |
| CN106007240B (en) | 2021-04-27 |
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