CN106109567A - The preparation method of anti-EV 71 virus after a kind of Herba Pogostemonis and Holotrichia diomphalia Bates compatibility of effective components - Google Patents
The preparation method of anti-EV 71 virus after a kind of Herba Pogostemonis and Holotrichia diomphalia Bates compatibility of effective components Download PDFInfo
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- CN106109567A CN106109567A CN201610558452.0A CN201610558452A CN106109567A CN 106109567 A CN106109567 A CN 106109567A CN 201610558452 A CN201610558452 A CN 201610558452A CN 106109567 A CN106109567 A CN 106109567A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/532—Agastache, e.g. giant hyssop
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention belongs to the separation of active ingredient of Chinese herbs extraction process and antivirus effective position, relate generally to the separation method of anti-EV 71 virus effective site after Herba Pogostemonis and grub extract compatibility, concretely comprise the following steps employing distilled water reflux, extract, Herba Pogostemonis, Holotrichia diomphalia Bates, precipitate with ethanol, antiviral, dissolution precipitation, uses the macroporous adsorbent resin of D101 model grub extract to carry out separating effect preferably, determines antivirus effective position.Then by the effective site of two kinds of medicines, respectively in 9:1,8:2,7:3,6:4,5:5,4:6,3:7,2:8,1:9 ratio compatibility, anti-EV 71 virus research is again carried out.The method that the present invention uses antiviral to follow the trail of be found that Herba Pogostemonis and Holotrichia diomphalia Bates antivirus effective position after compatibility, antiviral effect is preferable, and finds optimal proportion compatibility.
Description
Technical field
The present invention relates to active ingredient of Chinese herbs produce extraction process, relate to Herba Pogostemonis, Holotrichia diomphalia Bates antivirus effective position extract
New approaches new method and at the new application of pharmaceutical field, particularly relates to the mode extraction separation and purification leaves of pulse plants that antiviral is followed the trail of
Fragrant, the effective ingredient of Holotrichia diomphalia Bates anti respiratory syncytial virus, and effective ingredient carries out the antiviral study after compatibility.
Background technology
The unexpected invasion of the most various viruses, such as SARS virus, H1N1 etc., virus is quick-fried each time
Send out and the fear of the mankind all can be caused the most dead, so finding suitable antiviral drugs most important.
Herba Pogostemonis is that Labiatae stings stamen grass platymiscium, and it originates in south east asia, such as Malaysia, Philippine, India Buddhist nun
The states such as West Asia, China's introducing and planting is with a long history, can trace back to beam generation or former.
Enterovirus belongs to the enterovirus group in Parvoviridae in virusology, EV-71 be in current enterovirus group
The virus that evening finds, its infectious pole and strong and pathogenicity rate is higher, the especially complication in terms of nervous system.EV-71
Virus is one of main pathogens causing Children, it is possible to cause aseptic meningitis, brain stem encephalitis and spinal cord
The multiple nervous system disease such as the paralysis of poliomyelitis sample
Herba Pogostemonis has eliminating turbid pathogen with aromatics, delivers expelling summer-heat, effect of appetizing preventing or arresting vomiting, is usually used in treating turbid damp obstructing in middle-JIAO, the vomiting of gastral cavity painful abdominal mass, heat-damp in summer
Asthenia, cold-damp close the disease such as summer-heat, not easypro, stomachache vomiting and diarrhoea uncomfortable in chest.
The extract of Herba Pogostemonis has the effect of resisiting influenza virus and Respirovirus.
Holotrichia diomphalia Bates is the larva of the black chafer of chafer larva Korea, is about 35mm, milky, and body often bends, close SHENGHUANG
White fine, soft fur, moves in 3～6cm deep soil, stings the root of food crops, the most agriculturally it as the insect of crops
Catch and kill；But medically there is multiple pharmacological effect, such as removing blood stasis, clots absorbing, eliminating stagnation lactogenesis etc., with control lose, pain of becoming silted up, gout,
Tetanus, sore throat, carbuncle etc..Holotrichia diomphalia Bates is distributed mainly on the ground such as Northeast China, North China, Korea.
In terms of pharmacology, grub extract has antitumor, the treatment multiple biological activity such as aphtha and ocular disease, is
The animal drugs that a kind of great research and development is worth.
Summary of the invention
The technical problem to be solved is to specify that Herba Pogostemonis and the effective site of Holotrichia diomphalia Bates anti-EV-71 virus, for this
The separation and Extraction of effective site provides a kind of mode utilizing antiviral to follow the trail of and prepares Herba Pogostemonis, Holotrichia diomphalia Bates antivirus effective position
New method, and find both optimal proportion compatibilities.
It is an advantage of the invention that the present invention to isolate first in Herba Pogostemonis, Holotrichia diomphalia Bates and there is a kind of effective of anti-EV-71 virus
Position, and invented a kind of method that antiviral follows the trail of prepared by this effective site, this separation method purposiveness is strong, letter
Singly save substantial amounts of manpower and materials, be better than traditional system and separate.EV-71 virus is had relatively by the effective site prepared
Good therapeutical effect.
Herba Pogostemonis of the present invention and the optimal proportion compatibility of Holotrichia diomphalia Bates effective site antiviral are 2:3 position and optimal proportion compatibility
Preparation method, comprises the steps:
(1) a certain amount of Herba Pogostemonis, Holotrichia diomphalia Bates medical material are taken respectively by the water heating and refluxing extraction 2 times of 10 times, 2 hours for the first time second time
1.5 hour.
(2) merge the extracting solution concentrating under reduced pressure of twice, be concentrated into the medical material that every milliliter of medicinal liquid contains 1 gram, then add ethanol
The concentration of regulation ethanol is 70%, stands 24 hours.It is filtrated to get precipitation and supernatant.
(3) supernatant is reclaimed ethanol, by precipitation water dissolution, carry out Antiviral breeding respectively.Experiment display precipitation
Antiviral effect is preferable.
(4) take certain D101, HPD100, DM130, X-5, AB-8 model macroporous adsorbent resin respectively with 95% ethanol
Soak 24 hours, fill post afterwards, be washed till without alcohol taste with distilled water, be then rinsed with the aqueous hydrochloric acid solution of 5 times of column volumes 5%
Soak 3 hours, afterwards with distilled water punching to PH equal to 7, then be rinsed soaking with the NaOH aqueous solution of the 5% of 5 times of column volumes
3 hours, afterwards with distilled water punching to PH equal to 7, standby.
(5) take the precipitation in (3) and be splined on the macroporous resin column handled well, respectively with water, 25% ethanol, 50% ethanol, 75%
Ethanol is rinsed, and in each eluent, every 200ml connects one bottle, and changes next eluting after being flushed to not have color again
Liquid.
(6) the above-mentioned sample received is carried out Antiviral breeding.Experiment display is through the screening of macroporous resin, Herba Pogostemonis pair
The effect of AB-8 macroporous resin is preferable, and Holotrichia diomphalia Bates is preferable to D101 macroporous adsorbent resin effect, Herba Pogostemonis and Holotrichia diomphalia Bates water position antiviral
Effect is fine.
(7) the water position of Herba Pogostemonis with Holotrichia diomphalia Bates is entered with the ratio of 9:1,8:2,7:3,6:4,5:5,4:6,3:7,2:8,1:9 respectively
Row compatibility, result displaying ratio 2:3 is optimal proportion compatibility.
Detailed description of the invention
Example 1(1) weigh a certain amount of Herba Pogostemonis, Holotrichia diomphalia Bates and add 10 times amount heating and refluxing extraction respectively twice, 2 hours for the first time,
Secondary 1.5 hours, merges twice extracting solution, is evaporated to the medical material that every milliliter of medicinal liquid contains 1 gram, adds ethanol regulation solution second
The concentration of alcohol is 70%, stands 24 hours.
(2) the alcohol deposit fluid decompression sucking filtration (1) obtained, is precipitated, precipitation is used distilled water ultrasonic dissolution.
(3) solution that (2) obtain is splined on D101, HPD100, DM130, X-5, AB-8 macroporous absorption tree handled well
Fat, is rinsed with water, and every 200ml connects one bottle, rinses to colourless.
(4) merge water elution position in (3), be concentrated into certain volume, carry out antiviral study respectively.
(5) by the agastache rugosa water eluting position through AB-8 Resin sieving selection obtained in (4) and through D101 Resin sieving selection
Holotrichia diomphalia Bates water elution position carries out compatibility with the ratio of 9:1,8:2,7:3,6:4,5:5,4:6,3:7,2:8,1:9 respectively, again enters
Row Antiviral breeding, after result display Herba Pogostemonis and Holotrichia diomphalia Bates compatibility, antiviral effect is preferable, and ratio 2:3 is optimal compatibility ratio
Example.
Claims (10)
1. a Herba Pogostemonis and grub extract have the effective site of anti-EV-71 virus function after compatibility and a kind of utilization resists
The process of mode this effective ingredient of extraction separation and purification that virus is followed the trail of, it is characterised in that Herba Pogostemonis, the water extract-alcohol precipitation of Holotrichia diomphalia Bates
Precipitation position have suppression EV-71 virus effect, first with water extract-alcohol precipitation, next utilizes AB-8(Herba Pogostemonis), D101(Qi
Scarabaeiform) macroporous resin of model separates, each step extract separate after antiviral to be carried out track out the place of effective site,
Again the position having antivirus action is carried out compatibility, finally carry out Antiviral breeding research, draw optimal compatibility program.
Effective site the most according to claim 1 is characterized in that the precipitation position that this effective site is water extract-alcohol precipitation, warp
After macroporous resin separates, water position is the effective site of Herba Pogostemonis and Holotrichia diomphalia Bates antiviral, and the optimal proportion compatibility at water position is 2:3.
Extraction separation method the most according to claim 1, it is characterised in that the solid-liquid ratio of agastache rugosa water reflux, extract, is 1:10, carries
Taking number of times twice, extraction time is 2 hours for the first time, 1.5 hours for the second time.
Extraction separation method the most according to claim 1, it is characterised in that the concentrated solution concentration of Aqueous extracts is 1g/ml, precipitate with ethanol
Concentration is 70%, and the precipitate with ethanol time is 24 hours.
Extraction separation method the most according to claim 1, it is characterised in that carry out after precipitate with ethanol reduce pressure sucking filtration, be precipitated and
Supernatant, then carries out Antiviral breeding and is precipitated as antivirus effective position.
Extraction separation method the most according to claim 1, it is characterised in that Herba Pogostemonis is divided with the macroporous resin model used by Holotrichia diomphalia Bates
Not Wei AB-8, D101, eluent is water, 25% ethanol, 50% ethanol, 75% ethanol, and every 200ml connects one bottle, each gradient rush to nothing
Color.
Extraction separation method the most according to claim 1, it is characterised in that the eluent of Herba Pogostemonis extract is carried out antiviral
Experiment, obtaining water position is effective site.
Extraction separation method the most according to claim 1, it is characterised in that the eluent of grub extract is carried out antiviral
Experiment, obtaining water position is effective site.
Extraction separation method the most according to claim 1, it is characterised in that by agastache rugosa water position with Holotrichia diomphalia Bates water position by certain
Ratio compatibility, proportion compatibility is respectively 9:1,8:2,7:3,6:4,5:5,4:6,3:7,2:8,1:9.
Extraction separation method the most according to claim 1, it is characterised in that optimal antiviral proportion compatibility is 2:3.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610558452.0A CN106109567A (en) | 2016-07-16 | 2016-07-16 | The preparation method of anti-EV 71 virus after a kind of Herba Pogostemonis and Holotrichia diomphalia Bates compatibility of effective components |
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| CN201610558452.0A CN106109567A (en) | 2016-07-16 | 2016-07-16 | The preparation method of anti-EV 71 virus after a kind of Herba Pogostemonis and Holotrichia diomphalia Bates compatibility of effective components |
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| CN106109567A true CN106109567A (en) | 2016-11-16 |
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| CN201610558452.0A Pending CN106109567A (en) | 2016-07-16 | 2016-07-16 | The preparation method of anti-EV 71 virus after a kind of Herba Pogostemonis and Holotrichia diomphalia Bates compatibility of effective components |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101780239A (en) * | 2009-01-15 | 2010-07-21 | 颜平安 | Four-herb anti-tumor and anti-AIDS soft capsules and preparation process thereof |
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2016
- 2016-07-16 CN CN201610558452.0A patent/CN106109567A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101780239A (en) * | 2009-01-15 | 2010-07-21 | 颜平安 | Four-herb anti-tumor and anti-AIDS soft capsules and preparation process thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王清等: "藿香抗肠道EV-71病毒的体外筛选", 《辽宁中医杂志》 * |
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