CN106107051A - A kind of method producing high protein feed for raw material secondary mixed fungus fermentation with distiller's grains of beer - Google Patents
A kind of method producing high protein feed for raw material secondary mixed fungus fermentation with distiller's grains of beer Download PDFInfo
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- CN106107051A CN106107051A CN201610501491.7A CN201610501491A CN106107051A CN 106107051 A CN106107051 A CN 106107051A CN 201610501491 A CN201610501491 A CN 201610501491A CN 106107051 A CN106107051 A CN 106107051A
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- beer
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- distiller
- bacillus subtilis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of with distiller's grains of beer be the method that raw material secondary mixed fungus fermentation produces high protein feed, including following preparation process: (1) with the proportional arrangement of 6 10:2, adds distiller's grains of beer and wheat bran the water of 13 times, carries out sterilizing after mixing；(2) carry out mixed bacterium inoculation to carry out fermenting for the first time；(3) for the first time after fermentation ends, then add ammonium sulfate 3% 7%, carbamide 1 3%, carry out second time after mixed bacterium inoculation and ferment and i.e. can get high protein feed.The inventive method, during fermenting, is undertaken in two steps adding strain, reaches required purpose step by step, first reduces coarse-fibred content in culture medium, then improve the content of protein in culture medium, can preferably produce required high protein feed.
Description
Technical field
The present invention relates to a kind of method producing high protein feed for raw material secondary mixed fungus fermentation with distiller's grains of beer, belong to feedstuff
Manufacture field.
Background technology
In recent years, the method for domestic and international commonly used drying preserves distiller's grains of beer, although efficiently solve distiller's grains of beer easy
Corrupt problem, reduces environmental pollution, simultaneously facilitates accumulating.But owing to the crude fiber content in distiller's grains of beer is too high, animal is agreeable to the taste
Property poor, use that face is little, the market price is the highest, add the factors such as equipment investment, artificial and energy consumption, occur in that to sell fresh grain more dry than selling
The situation that grain is also made money, some beer company occurs that drying plant leaves unused.
Distiller's grains of beer are directly as feedstuff, the always hot issue of research.Deng Qihua[1]Distiller's grains of beer are produced pig feed close
Key technology is studied, and uses preconditioning technique, enzymolysis and the fiber content of probiotics fermention technology degraded distiller's grains of beer, improves
Protein quality, improves the Feeding Value of distiller's grains of beer.So that sugar is poor and distiller's grains of beer are as raw material, access yeast[2], many bacterial strains optimization group
Close such as bacillus subtilis, candida tropicalis solid state process fermenting and producing high protein feed[3-6], and containing multiple digestive enzyme.Profit
With Response surface methodology, the condition that optimization distiller's grains of beer solid fermentation is prepared albumen feedstuff is further studied[7]。
Sun Danfeng[8]Carrying out the feeding experiment such as pig, chicken Deng to fermented brewer's grain feedstuff, result shows respond well.
At present, there are no distiller's grains of beer secondary mixed fungus fermentation technology and produce the relevant report of high protein distiller's grains of beer feedstuff.
List of references:
[1] Deng Qihua. distiller's grains of beer produce pig feed key technology research [D]. Urumchi: Xinjiang Agricultural Univ, 2010
[2] Guo Jianhua, Dou Shaohua, Qiu Ran, etc. utilize sugar grain and distiller's grains of beer to produce the research [J] of albumen feedstuff. feed industry,
2005,26(1):4-5
[3] Cai Jun. the research [J] of Producing Protein Feed from Brewers Grain Through Fermentation. grain and feed industries, 2001, (3): 30-31
[4] Tao Min, Wang Weijia, Cai Jun. the compatibility [J] of high protein feed strain is produced in distiller's grains of beer fermentation. feed industry .2011,32
(11):58-61
[5] Wang Ying, Ma Haile. mixed culture solid state fermentation distiller's grains of beer produce the research [J] of albumen feedstuff. feed industry, 2010,31
(17):26-28
[6] Wang Chengtao, Zhu Guihua, Ma Yujun, etc. the optimization of the fermentation condition of grain slag fermentation production of fodder albumen and effect [J].
Heilongjiang Bayi Agricultural Reclamation University's journal, 2002,14 (3): 72-75
[7] An Xiaoping, Qi Jingwei, Ulan, etc. response surface optimization distiller's grains of beer solid fermentation prepares the condition research of albumen feedstuff
[J]. grain and feed industries .2013. (2): 40-43
[8] Sun Danfeng, Wang Youwei, Wang Cong, etc. fermented brewer's grain assessment of nutritional value and the impact [J] on growth of meat chicken performance.
Feed industry, 2009,30 (17): 26-28.
Summary of the invention
Distiller's grains of beer fermentation at present makes to make in solid-state mixed fungus fermentation, and strain once adds, and product fermentation certainly will be caused micro-life
Influencing each other between thing, for improving the service efficiency of distiller's grains of beer, the present invention uses, by choosing, the mycete that cellulose-decomposing ability is strong
Decomposition of cellulose, provides sugar source for subsequent fermentation, then adds yeast and bacillus subtilis, produces with mycete decomposition of cellulose
Sugar source ferment further, reach to make full use of cellulose, the one of the purpose improving protein output with distiller's grains of beer is
Raw material secondary mixed fungus fermentation produces the method for high protein feed.
Present invention provide the technical scheme that a kind of with distiller's grains of beer for raw material secondary mixed fungus fermentation production high protein feed
Method, including following preparation process:
(1) by weight, distiller's grains of beer with wheat bran with the proportional arrangement of 6-10:2, add the water of 1-3 times, go out after mixing
Bacterium；
(2) after sterilizing, carrying out mixed bacterium inoculation and carry out fermenting for the first time, mixed bacterium inoculum concentration is 18-22%, and described mixed bacterium is aspergillus niger
And Trichoderma spp., cultivate 2-4 days at 28-32 DEG C；
(3) for the first time after fermentation ends, then ammonium sulfate 3%-7% is added, carbamide 1-3%, carry out second time after mixed bacterium inoculation and ferment,
Described mixed bacterium is beer yeast and bacillus subtilis, or feed yeast and bacillus subtilis, and inoculum concentration is 15%-18%,
Cultivate 3-5 days at temperature 30-35 DEG C, i.e. can get high protein feed.
Described method, it is preferable that in step (2), the ratio of aspergillus niger and Trichoderma spp. is 6:4；Beer yeast and hay bud
The ratio of the ratio of spore bacillus or feed yeast and bacillus subtilis is 5:1；In step (1), sterilising conditions is 115 DEG C,
15min。
Described method, it is preferable that in step (1), distiller's grains of beer and wheat bran, with the proportional arrangement of 8:2, add 2 times
Water；In step (2), mixed bacterium inoculum concentration is 18-22%, cultivates 3 days at 30 DEG C；In step (3), add ammonium sulfate 3.46%, urine
Element 2%, the inoculum concentration of beer yeast and bacillus subtilis is 16.26%, or feed yeast and bacillus subtilis inoculum concentration are
16.51%, cultivate 4 days at temperature 35 DEG C.
The method have the advantages that
The present invention, with distiller's grains of beer as raw material, utilizes aspergillus niger and koning trichoderma to carry out for the first time and ferments, cultivate 3 days at 30 DEG C
Time, cellulase activity is 731.603nkat.With the distiller's grains of beer of for the first time fermentation as raw material, add beer yeast and bacillus subtilis
Bacterium combination carries out second time mixed fungus fermentation, adds ammonium sulfate 3.46%, theoretical gross protein value 38.11%；Add feed yeast
When carrying out second time mixed fungus fermentation with bacillus subtilis combination, theoretical gross protein value 43.38%.The inventive method is being entered
During row fermentation, it is undertaken in two steps adding strain, reaches required purpose step by step, first reduce in culture medium coarse-fibred
Content, then improve the content of protein in culture medium, can preferably produce required high protein feed.
Accompanying drawing explanation
Fig. 1 is the Technology Roadmap of the present invention.
Detailed description of the invention
It is further elucidated with the present invention below by the detailed description of detailed description of the invention, but is not the limit to the present invention
System, only illustrates.
Embodiment one:
Refer to Fig. 1, the primary dcreening operation of thalline: determine whether strain can utilize the nutritional labeling of distiller's grains of beer to grow.
250mL triangular flask loads treated distiller's grains of beer and wheat bran 30g(altogether takes medicated beer groove to be 8 with the ratio of wheat bran:
2), as fermentation medium.
In the beaker of 250mL, add fermentation medium 30g, add 0.5 times of distilled water, after 115 DEG C of sterilizing 15min,
Access the seed liquor (mixed bacterium is proportional allocations) of 20%, cultivate 3 days under 32 DEG C of cultivation temperature.
When the fermentation culture conditions used is consistent, the strain accessed be respectively aspergillus niger, koning trichoderma, aspergillosis,
Penicillium sp, koning trichoderma, aspergillus niger and koning trichoderma, aspergillus niger and penicillium sp, koning trichoderma and aspergillosis, koning trichoderma and penicillium sp, song
Mould and penicillium sp, aspergillus niger and koning trichoderma and aspergillosis, aspergillus niger and koning trichoderma and penicillium sp, koning trichoderma and aspergillosis and penicillium sp,
Aspergillus niger and aspergillosis and penicillium sp, calculate respectively and access different strain, the amount of the cellulase activity that culture medium produces.
The suitable strain selected, carries out further Experiment of Compatibility.
Excellent distiller's grains of beer fermentation strain compatibility and optimization: with distiller's grains of beer as main medium, with cellulase total activity be
Index, selects have good fiber element enzyme high yield fungal strain, and becomes excellent flora by compatibility；With protein content for referring to
Mark, the most excellent distiller's grains of beer yeast strain, and become excellent flora with bacillus cereus optimization blends.
Two kinds of different yeast are carried out response surface experiment respectively, draws optimal fermented bacterium.
Mixed fungus fermentation condition selection and optimization: with reducing sugar conversion coefficient as inspection target, by fiber bacterium inoculum concentration,
Fermentation time, fermentation temperature, amount of water carry out L9 (34) orthogonal, determine optimum process condition.
In single bacterium is fermented, with fermentation time, water content, inoculum concentration and temperature as test factor, carry out four factors three
The orthogonal optimization test of level.During vinasse fermentation, product coarse fiber content is as inspection target.
For investigating mixed fungus fermentation effect, with fermentation time, water content, strain ratio, inoculum concentration and temperature as test factor, enter
The Orthogonal Optimization Test of row five factor four level.During vinasse fermentation, product coarse fiber content is as inspection target.
By SPSS software (IBM SPSS Statistics 22), orthogonal experiments is analyzed, draws optimal work
Skill condition.
Secondary mixed fungus fermentation condition selection and optimization: according to the determination factor of influence of the nutritional labeling of distiller's grains of beer, carry out
Plackett-Burman EXPERIMENTAL DESIGN.Utilize the design of the Box-Behnken in response phase method that important factor is carried out further
Optimization, and carry out the checking of condition of culture.
Plackett-Burman designs: by pre-stage test, choose affect tunning gross protein value possible because of
Element, including ammonium sulfate, carbamide, inoculum concentration, strain ratio, temperature, time.In order to these 6 factors are investigated comprehensively, select
Plackett-Burman designs, and each factor takes 2 levels, and gross protein value is as response value Y.Investigate the master of each factor
Effect and reciprocal action, determine important factor in order.
Obtain from experimental result, affect the Main Factors of gross protein value, then enter next step with these 3 factors
Response surface analysis is tested.Response surface analysis: according to the center combination design principle of Box-Behnken, to Plackett-Burman
3 major influence factors of EXPERIMENTAL DESIGN screening, carry out Box-Behnken EXPERIMENTAL DESIGN.According to Box-Behnken center combination
Design principle, with gross protein value as response value, designs the response surface analysis test of 3 factor 3 level totally 15 testing sites.?
Afterwards by Minitab software processes, obtain optimised process.
(the training as raw material after mixing with distiller's grains of beer (offer of Yanjing Brewery (Hengyang) company limited) and wheat bran by weight 8:2
Support base), utilize aspergillus niger (Aspergillus niger) (Sheng Ke institute of Agricultural University Of Hunan laboratory provides) and koning trichoderma
(T.koningi AS3.2774) (Sheng Ke institute of Agricultural University Of Hunan laboratory provides) carries out one time fermentation, by weight, fermentation
Time amount of water be 2 times of gross weight of distiller's grains of beer and wheat bran, aspergillus niger: Trichoderma spp. be the mixed bacterium inoculum concentration of 6:4 be 20%(inoculum concentration
For culture volume 20%), when cultivating 3 days at 30 DEG C, cellulase activity is 731.603nkat.Beer with fermentation for the first time
Distiller grains are raw material, and to add ammonium sulfate 3.46%(addition be the 3.46% of culture volume), carbamide 2%(addition is culture medium
The 2% of volume), beer yeast (Sac-charomyces cerevisiae) (Sheng Ke institute of Agricultural University Of Hunan laboratory provide) with
It is mixed that bacillus subtilis (Bacillus subtilis) (Sheng Ke institute of Agricultural University Of Hunan laboratory provides) combination carries out second time
Bacterium is fermented, and optimum condition is temperature 35 DEG C, cultivates 4 days, and inoculum concentration 16.26%(inoculum concentration is the 16.26% of culture volume), beer
Brewer yeast is 5:1 than the ratio of bacillus subtilis, theoretical gross protein value 38.11%(Kjeldahl's method).
Embodiment two:
(cultivate as raw material after mixing with distiller's grains of beer (offer of Yanjing Brewery (Hengyang) company limited) and wheat bran by weight 8:2
Base), utilize aspergillus niger (Aspergillus niger) and koning trichoderma (T.koningi) to carry out one time fermentation, by weight,
During fermentation, amount of water is 2 times of distiller's grains of beer, aspergillus niger: Trichoderma spp. be 6:4 mixed bacterium inoculation 20%(inoculum concentration be culture volume
20%), when cultivating 3 days at 30 DEG C, cellulase activity is 731.603nkat.With the distiller's grains of beer of for the first time fermentation as raw material, add
Ammonium sulfate 5.00%, carbamide 2%, feed yeast (AS2.1180) (Hunan, Hunan light industry academy offer) and bacillus subtilis are provided
When (Bacillus subtilis) combination carries out second time mixed fungus fermentation, optimum condition is temperature 33.73 DEG C, cultivates 4 days, connects
Planting amount 16.51%, feed yeast is 5:1 than the ratio of bacillus subtilis, theoretical gross protein value 43.38%(kjeldahl determination
Method).
Claims (5)
1. the method producing high protein feed with distiller's grains of beer for raw material secondary mixed fungus fermentation, including following preparation process:
(1) by weight, distiller's grains of beer with wheat bran with the proportional arrangement of 6-10:2, add the water of 1-3 times, go out after mixing
Bacterium；
(2) after sterilizing, carrying out mixed bacterium inoculation and carry out fermenting for the first time, mixed bacterium inoculum concentration is 18-22%, and described mixed bacterium is aspergillus niger
And Trichoderma spp., cultivate 2-4 days at 28-32 DEG C；
(3) for the first time after fermentation ends, then ammonium sulfate 3%-7% is added, carbamide 1-3%, carry out second time after mixed bacterium inoculation and ferment,
Described mixed bacterium is beer yeast and bacillus subtilis, or feed yeast and bacillus subtilis, and inoculum concentration is 15%-18%,
Cultivate 3-5 days at temperature 30-35 DEG C, i.e. can get high protein feed.
Method the most according to claim 1, it is characterised in that: in step (2), the ratio of aspergillus niger and Trichoderma spp. is 6:4.
Method the most according to claim 1, it is characterised in that: in step (3), beer yeast and the ratio of bacillus subtilis
The ratio of example or feed yeast and bacillus subtilis is 5:1.
Method the most according to claim 1, it is characterised in that: in step (1), sterilising conditions is 115 DEG C, 15min.
Method the most according to claim 1, it is characterised in that: in step (1), distiller's grains of beer are joined with the ratio of 8:2 with wheat bran
Put, add the water of 2 times；In step (2), mixed bacterium inoculum concentration is 20%, cultivates 3 days at 30 DEG C；In step (3), add sulphuric acid
Ammonium 3.46%, carbamide 2%, the inoculum concentration of beer yeast and bacillus subtilis is 16.26%, or feed yeast and bacillus subtilis
Bacterium inoculum concentration is 16.51%, cultivates 4 days at temperature 35 DEG C.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900977A (en) * | 2016-12-30 | 2017-06-30 | 浙江海洋大学 | The method that feed is prepared using shrimping beam trawl |
| CN108185160A (en) * | 2017-12-04 | 2018-06-22 | 杨忠华 | A kind of preparation method of cattle feed |
| CN108902446A (en) * | 2018-06-20 | 2018-11-30 | 十堰真武酒业有限公司 | A kind of high protein lees feeds and preparation method thereof |
| CN109463572A (en) * | 2018-11-27 | 2019-03-15 | 广州科盛生物科技有限公司 | It is a kind of to enhance disease-resistant and immunocompetence feed stuff for pet fish |
| CN109964880A (en) * | 2019-03-25 | 2019-07-05 | 宜宾和美幸福科技有限公司 | For the kitchen feed and preparation method thereof of black soldier flies larva cultivation, black soldier flies larva cultural method |
| CN110583856A (en) * | 2019-10-23 | 2019-12-20 | 辽东学院 | Method for producing protein feed by carrying out two-time different mixed fermentation on refined brewer grains and blueberry pomace |
| CN110679785A (en) * | 2019-10-30 | 2020-01-14 | 博益德（北京）生物科技有限公司 | Fish brewer grain-miscellaneous meal type biological fermentation feed and preparation method and application thereof |
| CN111513207A (en) * | 2020-05-28 | 2020-08-11 | 衡阳师范学院 | Production method and use effect of biological feed capable of improving meat quality |
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- 2016-06-30 CN CN201610501491.7A patent/CN106107051A/en active Pending
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900977A (en) * | 2016-12-30 | 2017-06-30 | 浙江海洋大学 | The method that feed is prepared using shrimping beam trawl |
| CN108185160A (en) * | 2017-12-04 | 2018-06-22 | 杨忠华 | A kind of preparation method of cattle feed |
| CN108902446A (en) * | 2018-06-20 | 2018-11-30 | 十堰真武酒业有限公司 | A kind of high protein lees feeds and preparation method thereof |
| CN109463572A (en) * | 2018-11-27 | 2019-03-15 | 广州科盛生物科技有限公司 | It is a kind of to enhance disease-resistant and immunocompetence feed stuff for pet fish |
| CN109964880A (en) * | 2019-03-25 | 2019-07-05 | 宜宾和美幸福科技有限公司 | For the kitchen feed and preparation method thereof of black soldier flies larva cultivation, black soldier flies larva cultural method |
| CN110583856A (en) * | 2019-10-23 | 2019-12-20 | 辽东学院 | Method for producing protein feed by carrying out two-time different mixed fermentation on refined brewer grains and blueberry pomace |
| CN110679785A (en) * | 2019-10-30 | 2020-01-14 | 博益德（北京）生物科技有限公司 | Fish brewer grain-miscellaneous meal type biological fermentation feed and preparation method and application thereof |
| CN111513207A (en) * | 2020-05-28 | 2020-08-11 | 衡阳师范学院 | Production method and use effect of biological feed capable of improving meat quality |
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