CN106093377A - A kind of test kit for the quick coupling of albumen, antibody, enzyme and nucleic acid and preparation method thereof - Google Patents
A kind of test kit for the quick coupling of albumen, antibody, enzyme and nucleic acid and preparation method thereof Download PDFInfo
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- CN106093377A CN106093377A CN201610661895.2A CN201610661895A CN106093377A CN 106093377 A CN106093377 A CN 106093377A CN 201610661895 A CN201610661895 A CN 201610661895A CN 106093377 A CN106093377 A CN 106093377A
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- buffer
- antibody
- test kit
- albumen
- enzyme
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
Abstract
The present invention relates to a kind of test kit for the quick coupling of albumen, antibody, enzyme and nucleic acid, including being respectively provided with activation magnetic microsphere；Cleaning mixture；Coupling buffer；Block buffer；Preserve the test tube of buffer.Wherein, the preparation process of activation magnetic microsphere includes that (1) respectively takes 500mL N, and 4A molecular sieve crossed by N dimethyl acetylamide (DMAC) and isopropanol；(2) take carboxyl magnetic bead DMAC to wash two to three times；(3) carboxyl magnetic bead after washing adds DMAC and N N-Hydroxysuccinimide, 40 DEG C of reaction 0.5h；(4) collecting reaction product, washes twice with isopropanol, is eventually adding isopropanol and is configured to the activated magnetic beads suspension of 10mg/mL, 4 DEG C of preservations.The present invention solves user complex operation, complicated difficult problem during magnetic bead activation, ligand coupling, magnetic bead closing etc., simplifies operating procedure, it is achieved the Appropriate application of resource.
Description
Technical field
The invention belongs to biotinylation kit Material Field, be specifically related to a kind of for albumen, antibody, enzyme and nucleic acid coupling
Test kit and be equipped with method, can quickly, simply, efficiently by albumen, antibody, enzyme, amido modified nucleic acid and other band ammonia
The molecule covalent of base is coupled to activate on magnetic microsphere, and the magnetic bead after coupling part can be used for detecting, isolated and purified etc..
Background technology
Immunomagnetic beads is the new immunological technique that development in recent years is got up, it by distinctive for solidified reagents advantage with
The high degree of specificity of immunological response be incorporated into one, based on immunology, penetrate into pathology, physiology, pharmacology, microorganism,
The every field such as biochemistry and molecular genetics, it is at immune detection, cell separation, biological macromolecule purifying and molecular biology
Etc. aspect obtained increasingly being widely applied.
Use the preparation method of Core-Shell Particles, with monodispersed polymer microballoon as template, magnetisable material is deposited
Internal in template microsphere or be wrapped in template microsphere surface, synthesis covers inorganic matter or high score containing superparamagnetism material, surface
The composite of sub-polymer.This kind of composite has superparamagnetism, it is possible to realize solid-liquid separation under the action of a magnetic field, is dividing
Analysis detection easily coordinate instrument realize full-automatic operation；The most this kind of composite also has homogeneous particle diameter, huge
Surface area, excellent stability, utilize the functional group such as amino, carboxyl, aldehyde radical, sulfydryl etc. of these material surfaces to resist
The covalent coupling of body, can be used for combining corresponding antigen or antibody, so can do displacement under the effect of externally-applied magnetic field, from
And the purpose of separation, detection and the purification to gene, protein, cell, microorganism etc. can be realized.It is at immune detection, base
Because the aspects such as extraction, protein purification, cell separation, microorganism enrichment have a wide range of applications.
Along with immunomagnetic beads is more and more extensive in field application such as medical science, biology, food safetys, asks the most real
Topic pendulum is in face of application person: the biomolecule that how can need, as the simplicity such as albumen, antibody, enzyme, nucleic probe,
Covalent coupling is on magnetic bead fast and efficiently？Especially for the application personnel of the specialty such as medical science, biology, bromatology, due to
Lack the knowledge relevant to the chemical reaction such as group activation and coupling, therefore the group to magnetic bead surfaces be modified, activate and
Ligand coupling has just become their operation weakness.Utilize carboxyl magnetic bead coupled antibody the most in actual applications, be frequently encountered by
Problem just includes: use which kind of activator？The addition of activator, activation temperature and soak time are how many？Antibody coupling
What condition is？From the point of view of layman or abecedarian, these Considerations seriously constrain the dexterity of operation.
For problem above, can be by the Professional knowledge each grasped, developing one can fast and easy operation
Test kit, this test kit provides the reagent such as the buffer that the most activated good magnetic bead and each step need to use, is so
Make the operator being never did ligand coupling the most successfully by ligand coupling to magnetic bead, in order to subsequent applications, from
And avoid operator to consume too much energy on the operations such as magnetic bead activation, buffer and reaction condition screening, save
Human and material resources and time, improve operating efficiency, reduce operational error.
Summary of the invention
The technical problem to be solved be for above-mentioned prior art provide a kind of for albumen, antibody, enzyme and
The test kit of the quick coupling of nucleic acid and related reagent are equipped with method, can quickly, simply, efficiently by albumen, antibody, enzyme, amino
The nucleic acid modified and the molecule covalent of other band amino are coupled to activate on magnetic microsphere, and the magnetic bead after coupling part can be used for examining
Survey, isolated and purified etc..
The present invention solves the technical scheme that the problems referred to above are used: a kind of quickly even for albumen, antibody, enzyme and nucleic acid
The test kit of connection, including being respectively provided with activation magnetic microsphere；Cleaning mixture；Coupling buffer；Block buffer；Preserve buffer
Test tube.
The preparation method of mentioned reagent box, including following key step:
One, the preparation process of activation magnetic microsphere is as follows
(1) respectively take 500mL DMAC N,N' dimethyl acetamide (DMAC) and 4A molecular sieve crossed by isopropanol；
(2) take carboxyl magnetic bead DMAC to wash two to three times；
(3) carboxyl magnetic bead after washing adds DMAC and N-hydroxy-succinamide, 40 DEG C of reaction 0.5h；
(4) collecting reaction product, washes twice with isopropanol, be eventually adding isopropanol be configured to 10mg/mL activated magnetic beads hang
Supernatant liquid, 4 DEG C of preservations.
Two, the outfit process of cleaning mixture is, takes purified water 1L, and adding concentrated hydrochloric acid regulation pH is 2.5～5.0,0.22 μm filter
Membrane filtration, 4 DEG C of preservations.
Three, the outfit process of coupling buffer is: take 3-(N-morpholine) propane sulfonic acid (MOPS) 20.926g and sodium chloride
8.78g is dissolved in 1L purified water, and adding 5M sodium hydroxide solution regulation pH is 5.0～8.0,0.22 μm membrane filtration, 4 DEG C of guarantors
Deposit.
Four, the outfit process of Block buffer is: takes ethanolamine solutions 168.24g and is dissolved in 600mL purified water, adds dense
Salt acid for adjusting pH is 8.0～9.0, then adds purified water and is settled to 1L, last 0.22 μm membrane filtration, 4 DEG C of preservations.
Five, the outfit process preserving buffer is:
(1) NaCl 8g is weighed respectively, KCl 0.2g, Na2HPO412H2O 3.63g, KH2PO40.24g is in 2L beaker；
(2) measuring 900mL purified water in 2L beaker with graduated cylinder, Glass rod stirring makes solid fully dissolve；
(3) adjust pH value to 6.5～7.5 with 0.1M hydrochloric acid；
(4) taking 1mLproclin300 in 2L beaker with 1mL pipettor, Glass rod stirs；
(5) solution in beaker is poured in 1L volumetric flask, add purified water constant volume to 1L；
(6) by solution joined in e with 0.22 μm membrane filtration, 4 DEG C save backup.
The particle diameter of above-mentioned activation magnetic microsphere is 0.1～100 μm.
Activation magnetic microsphere Facing material is inorganic matter such as silicon dioxide, artificial-synthetic copolymer such as polystyrene, poly-first
Base glycidyl acrylate or natural polymer such as agarose, glucosan, cellulose.
Preferably, the content of activation magnetic microsphere surface active group N-hydroxy-succinamide is 20～2000 μm ol/
g。
In the present invention, cleaning mixture can also is that the 2-(N-morpholine of pH value 2.5～5.0) ethyl sulfonic acid aqueous solution or pH value
The MOPS solution of 2.5～5.0.
In the present invention, coupling buffer can also is that the 2-(N-morpholine of pH value 5.0～8.0) ethanesulfonic acid buffer, phosphoric acid
Salt buffer or carbonate buffer solution.
In the present invention, Block buffer can also is that Tris HCl buffer or the glycine solution of pH value 8.0～9.0.
Owing to surface active groups N-hydroxy-succinamide easily hydrolyzes, in traditional aqueous phase preparation process, N-hydroxyl
The formation of butanimide is carried out with hydrolysis simultaneously, is finally reached a kind of dynamic equilibrium, traditional aqueous phase synthesis method, N-hydroxyl
The content of the succinimide activated group of base is the most relatively low, is unfavorable for the coupling of follow-up part, and the hydrolysis of activated group is anti-simultaneously
Should also be unfavorable for activating the long-term preservation of magnetic microsphere, this is also equipped with a difficult problem present in process and preservation process, constrains
The development of related kit.The present invention, by using organic synthesis method to substitute traditional aqueous phase synthesis method, passes through molecular sieve simultaneously
Treatment technology removes the micro-moisture in organic solvent, has reached to prepare overactivity group content and realized activation magnetic microsphere long
The purpose that phase preserves, the test kit for preparation commercialization is laid a good foundation.
The beneficial effects are mainly as follows and provided the user a kind of test kit, interest concessions magnetic bead carry out albumen,
The operation of the quick coupling of antibody, enzyme and nucleic acid becomes a reality, and solves user and closes at magnetic bead activation, ligand coupling, magnetic bead
The problem encountered during Deng, simplifies operating procedure, it is achieved the Appropriate application of resource.
This test kit has the advantage that when carrying out albumen, antibody, enzyme and nucleic acid coupling
(1) simple to operate.Only part need to be dissolved in the coupling buffer that test kit carries, then with activated magnetic beads mixed at room temperature
0.5～1h just can be by part covalent coupling to magnetic bead, even the new hand never done also can be easily accomplished, and success rate
High；
(2) quick.Whole ligand coupling process about 2h just can complete, and the activation of the magnetic bead of routine, ligand coupling, magnetic bead were closed
Journey needs 5h, and the time has saved 60% than originally.
(3) scope of application is wider.It is suitable for albumen, antibody, enzyme, amido modified nucleic acid and the molecule of other band amino；
(4) ligand coupling efficiency and coupling amount are high.In the activation magnetic microsphere preparation process of core, by introducing organic being harmonious
Cheng Fa, prepares the activation magnetic microsphere of high N-hydroxy-succinamide content, improves coupling efficiency and the coupling of bio-ligand
Amount.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be described, for embodiment be only to product of the present invention or method
Make generality to illustrate, contribute to being more fully understood that the present invention, but be not limiting upon the scope of the invention.Described in following embodiment in fact
Proved recipe method, if no special instructions, is conventional method；Described material, if no special instructions, the most commercially obtains.
The test kit for the quick coupling of albumen, antibody, enzyme and nucleic acid of the present embodiment, including being respectively provided with activation magnetic
Microsphere；Cleaning mixture；Coupling buffer；Block buffer；Preserve the test tube of buffer.
The preparation method of mentioned reagent box, including following key step:
One, the preparation process of activation magnetic microsphere is as follows
(1) respectively take 500mL DMAC N,N' dimethyl acetamide (DMAC) and 4A molecular sieve crossed by isopropanol；
(2) take carboxyl magnetic bead DMAC to wash two to three times；
(3) carboxyl magnetic bead after washing adds DMAC and N-hydroxy-succinamide, 40 DEG C of reaction 0.5h；
(4) collecting reaction product, washes twice with isopropanol, be eventually adding isopropanol be configured to 10mg/mL activated magnetic beads hang
Supernatant liquid, 4 DEG C of preservations.
Two, the outfit process of cleaning mixture is, takes purified water 1L, and adding concentrated hydrochloric acid regulation pH is 2.5～5.0,0.22 μm filter
Membrane filtration, 4 DEG C of preservations.
Three, the outfit process of coupling buffer is: take 3-(N-morpholine) propane sulfonic acid (MOPS) 20.926g and sodium chloride
8.78g is dissolved in 1L purified water, and adding 5M sodium hydroxide solution regulation pH is 5.0～8.0,0.22 μm membrane filtration, 4 DEG C of guarantors
Deposit.
Four, the outfit process of Block buffer is: takes ethanolamine solutions 168.24g and is dissolved in 600mL purified water, adds dense
Salt acid for adjusting pH is 8.0～9.0, then adds purified water and is settled to 1L, last 0.22 μm membrane filtration, 4 DEG C of preservations.
Five, the outfit process preserving buffer is:
(1) NaCl 8g is weighed respectively, KCl 0.2g, Na2HPO412H2O 3.63g, KH2PO40.24g is in 2L beaker；
(2) measuring 900mL purified water in 2L beaker with graduated cylinder, Glass rod stirring makes solid fully dissolve；
(3) adjust pH value to 6.5～7.5 with 0.1M hydrochloric acid；
(4) taking 1mLproclin300 in 2L beaker with 1mL pipettor, Glass rod stirs；
(5) solution in beaker is poured in 1L volumetric flask, add purified water constant volume to 1L；
(6) by solution joined in e with 0.22 μm membrane filtration, 4 DEG C save backup.
Embodiment 1
Protein A coupling
1.Protein solution A is prepared: analytical balance weighs 10mg Protein A and is dissolved in the coupling that 10mL test kit carries and delays
Rush in liquid, be made into the Protein solution A of 1mg/mL；
2. activated magnetic beads washing: take 200mg and activate magnetic microsphere, the cleaning mixture quick wash carried with 10mL test kit 1 time,
Magnetic removes cleaning mixture after making magnetic bead adherent；
3. ligand coupling: in the Protein solution A of preparation joins the magnetic bead after washing in step 2 in step 1, pass through
After magnetic bead is mixed homogeneously with ligand solution by vibration, it is placed on mixed instrument, slowly rotates, room temperature reaction 1h, just can be by protein
A covalent coupling is on magnetic bead；
4. activated magnetic beads is closed: the bead suspension of Protein A is placed on magnetic separation rack by coupling in step 3, magnetic
After making magnetic bead adherent, remove Protein solution A.Add the Block buffer that 20mL test kit carries, by vibration by magnetic bead
Mix homogeneously with Block buffer, and be placed on mixed instrument, slowly rotate, room temperature reaction 1h, to activation remaining on magnetic bead
Site is closed；
5. washing and preservation: the bead suspension after closing in step 4 is placed on magnetic separation rack, and magnetic makes magnetic bead adherent
After, remove Block buffer.It is subsequently adding the preservation buffer quick wash 1 time that 20mL test kit carries, repeats above-mentioned washing
Step is once；
6. magnetic bead is configured to the bead suspension of certain working concentration by the last preservation buffer in right amount that again adds, and is placed in 4 DEG C
Save backup.
Embodiment 2
Carcinoembryonic antigen (CEA) antibody coupling
1.CEA solution is prepared: being made into 0.2mg/mL in the coupling buffer carried by CEA antibody test kit；
2. activated magnetic beads washing: take 10mg and activate magnetic microsphere, the cleaning mixture quick wash carried with 1mL test kit 1 time, magnetic
Suction removes cleaning mixture after making magnetic bead adherent；
3. ligand coupling: in the CEA solution 0.5mL of preparation joins the magnetic bead after washing in step 2 in step 1, by shaking
Swing after magnetic bead is mixed homogeneously with ligand solution, be placed on mixed instrument, slowly rotate, room temperature reaction 1h, just can be by antibody covalency
It is coupled on magnetic bead；
4. activated magnetic beads is closed: the bead suspension of CEA is placed on magnetic separation rack by coupling in step 3, and magnetic makes magnetic bead
After adherent, remove CEA solution.It is subsequently adding the Block buffer that 1mL test kit carries, by vibration, magnetic bead is buffered with closing
After liquid mix homogeneously, it is placed on mixed instrument, slowly rotates, room temperature reaction 1h, closes activated sites point remaining on magnetic bead；
5. washing and preservation: the bead suspension after closing in step 4 is placed on magnetic separation rack, and magnetic makes magnetic bead adherent
After, remove Block buffer.The preservation buffer quick wash that then addition 1mL test kit carries 1 time, repeats above-mentioned purge step
The most once；
6. it is eventually adding the appropriate buffer that preserves and magnetic bead is configured to the bead suspension of certain working concentration, be placed in 4 DEG C of preservations
Standby.
Embodiment 3
Amido modified nucleic probe coupling
1. nucleic probe solution preparation: take nucleic probe amido modified for 30nmol and be dissolved in the coupling that 150 μ L test kits carry and delay
Rush in liquid；
2. activated magnetic beads washing: take 10mg and activate magnetic microsphere, the cleaning mixture quick wash carried with 1mL test kit 1 time, magnetic
Suction removes cleaning mixture after making magnetic bead adherent；
3. ligand coupling: in the nucleic probe solution 150 μ L of preparation joins the magnetic bead after washing in step 2 in step 1,
After magnetic bead being mixed homogeneously with ligand solution by vibration, it is placed on mixed instrument, slowly rotates, room temperature reaction 1h, just can will join
Body covalent coupling is on magnetic bead；
4. activated magnetic beads is closed: the bead suspension of nucleic probe is placed on magnetic separation rack by coupling in step 3, and magnetic makes
After magnetic bead is adherent, remove nucleic probe solution.It is subsequently adding the Block buffer that 1mL test kit carries, by vibration by magnetic bead
After mixing homogeneously with Block buffer, being placed on mixed instrument, slowly rotate, room temperature reaction 1h, to activated sites remaining on magnetic bead
Point is closed；
5. washing and preservation: the bead suspension after closing in step 4 is placed on magnetic separation rack, and magnetic makes magnetic bead adherent
After, remove Block buffer.It is subsequently adding the preservation buffer quick wash 1 time that 1mL test kit carries, repeats above-mentioned purge step
The most once；
6. magnetic bead is configured to the bead suspension of certain working concentration by the last preservation buffer in right amount that again adds, and is placed in 4 DEG C
Save backup.
In addition to the implementation, present invention additionally comprises other embodiments, all employing equivalents or equivalence to replace
The technical scheme that mode is formed, all should fall within the scope of the hereto appended claims.
Claims (9)
1. one kind is used for albumen, antibody, enzyme and the test kit of the quick coupling of nucleic acid, it is characterised in that: include being respectively provided with activation
Magnetic microsphere；Cleaning mixture；Coupling buffer；Block buffer；Preserve the test tube of buffer.
2. the preparation side of the test kit for the quick coupling of albumen, antibody, enzyme and nucleic acid according to claim 1
Method, it is characterised in that: comprise the steps,
One, the preparation process of activation magnetic microsphere is as follows
(1) respectively take 500mL DMAC N,N' dimethyl acetamide (DMAC) and 4A molecular sieve crossed by isopropanol；
(2) take carboxyl magnetic bead DMAC to wash two to three times；
(3) carboxyl magnetic bead after washing adds DMAC and N-hydroxy-succinamide, 40 DEG C of reaction 0.5h；
(4) collecting reaction product, washes twice with isopropanol, be eventually adding isopropanol be configured to 10mg/mL activated magnetic beads hang
Supernatant liquid, 4 DEG C of preservations；
Two, the outfit process of cleaning mixture is, takes purified water 1L, and adding concentrated hydrochloric acid regulation pH is 2.5～5.0,0.22 μm filter membrane mistake
Filter, 4 DEG C of preservations；
Three, the outfit process of coupling buffer is: take 3-(N-morpholine) propane sulfonic acid (MOPS) 20.926g and sodium chloride 8.78g molten
In 1L purified water, adding 5M sodium hydroxide solution regulation pH is 5.0～8.0,0.22 μm membrane filtration, 4 DEG C of preservations；
Four, the outfit process of Block buffer is: takes ethanolamine solutions 168.24g and is dissolved in 600mL purified water, adds concentrated hydrochloric acid
Regulation pH is 8.0～9.0, then adds purified water and is settled to 1L, last 0.22 μm membrane filtration, 4 DEG C of preservations；
Five, the outfit process preserving buffer is:
(1) NaCl 8g is weighed respectively, KCl 0.2g, Na2HPO412H2O 3.63g, KH2PO40.24g is in 2L beaker；
(2) measuring 900mL purified water in 2L beaker with graduated cylinder, Glass rod stirring makes solid fully dissolve；
(3) adjust pH value to 6.5～7.5 with 0.1M hydrochloric acid；
(4) taking 1mLproclin300 in 2L beaker with 1mL pipettor, Glass rod stirs；
(5) solution in beaker is poured in 1L volumetric flask, add purified water constant volume to 1L；
(6) by solution joined in e with 0.22 μm membrane filtration, 4 DEG C save backup.
The most according to claim 2, be used for the preparation method of the test kit of albumen, antibody, enzyme and the quick coupling of nucleic acid, it is special
Levy and be: the particle diameter of described activation magnetic microsphere is 0.1～100 μm.
4. according to the preparation method of the test kit being used for albumen, antibody, enzyme and the quick coupling of nucleic acid described in Claims 2 or 3, its
It is characterised by: described activation magnetic microsphere Facing material is inorganic matter, artificial-synthetic copolymer or natural polymer.
The most according to claim 4, be used for the preparation method of the test kit of albumen, antibody, enzyme and the quick coupling of nucleic acid, it is special
Levying and be: described inorganic matter is silicon dioxide, described artificial-synthetic copolymer is polystyrene, polymethyl acid glycidyl
Ester, described natural polymer is agarose, glucosan, cellulose.
6. according to the preparation method of the test kit being used for albumen, antibody, enzyme and the quick coupling of nucleic acid described in Claims 2 or 3, its
It is characterised by: the content of described activation magnetic microsphere surface active group N-hydroxy-succinamide is 20～2000 μm ol/g.
The most according to claim 2, be used for the preparation method of the test kit of albumen, antibody, enzyme and the quick coupling of nucleic acid, it is special
Levy and be: described cleaning mixture can also is that the 2-(N-morpholine of pH value 2.5～5.0) ethyl sulfonic acid aqueous solution or pH value 2.5～5.0
MOPS solution.
The most according to claim 2, be used for the preparation method of the test kit of albumen, antibody, enzyme and the quick coupling of nucleic acid, it is special
Levy and be: described coupling buffer can also is that the 2-(N-morpholine of pH value 5.0～8.0) ethanesulfonic acid buffer, phosphate-buffered
Liquid or carbonate buffer solution.
The most according to claim 2, be used for the preparation method of the test kit of albumen, antibody, enzyme and the quick coupling of nucleic acid, it is special
Levy and be: described Block buffer can also is that Tris HCl buffer or the glycine solution of pH value 8.0～9.0.
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| CN107462708A (en) * | 2017-06-28 | 2017-12-12 | 迈克生物股份有限公司 | Method, application and the kit of antigen or antibody coating magnetic particle |
| CN107988207A (en) * | 2017-12-26 | 2018-05-04 | 济南凯晨科技有限公司 | Extracted from complex samples and purify the kit and method of salmonella DNA |
| CN108279303A (en) * | 2018-01-31 | 2018-07-13 | 珠海丽珠试剂股份有限公司 | A kind of method of antibody coupling carboxylation magnetic bead |
| CN110699427A (en) * | 2019-10-25 | 2020-01-17 | 江苏为真生物医药技术股份有限公司 | Carboxyl magnetic bead coupling modified nucleic acid probe and preparation method and application thereof |
| CN111013508A (en) * | 2020-01-02 | 2020-04-17 | 金丽素 | Preparation method of carboxyl biological magnetic beads |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107462708A (en) * | 2017-06-28 | 2017-12-12 | 迈克生物股份有限公司 | Method, application and the kit of antigen or antibody coating magnetic particle |
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| CN110699427A (en) * | 2019-10-25 | 2020-01-17 | 江苏为真生物医药技术股份有限公司 | Carboxyl magnetic bead coupling modified nucleic acid probe and preparation method and application thereof |
| CN111013508A (en) * | 2020-01-02 | 2020-04-17 | 金丽素 | Preparation method of carboxyl biological magnetic beads |
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