CN107167586A - A kind of antistreptolysin O (ASO) detection kit and preparation method thereof - Google Patents
A kind of antistreptolysin O (ASO) detection kit and preparation method thereof Download PDFInfo
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- CN107167586A CN107167586A CN201710547376.8A CN201710547376A CN107167586A CN 107167586 A CN107167586 A CN 107167586A CN 201710547376 A CN201710547376 A CN 201710547376A CN 107167586 A CN107167586 A CN 107167586A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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Abstract
The invention discloses a kind of antistreptolysin O (ASO) detection kit, the step of prior art is required to centrifugation or ultrafiltration is solved, wastes time and energy, is unfavorable for industrialized problem.The present invention includes reagent two, and the reagent two is to use after latex, buffer solution, EDC and HEPES hybrid reactions, adds NaOH regulation pH to 7.00~8.00, finally adds the finished product being made after SLO, sealer I and sealer II；The concentration of each material is in finished product：Latex 58.00ml/L~62.00ml/L；Buffer solution 140.00ml/L；EDC 0.0525g/L~0.0924g/L；HEPES 5.9578g/L；SLO 74.00mg/L~78.00mg/L；Sealer I 20.00ml/L；Sealer II 10.00ml/L.Present invention, avoiding the impurity elimination process such as the centrifugation of conventional latex sensitization process, ultrafiltration, production efficiency is substantially increased, time cost and human cost has been saved.
Description
Technical field
The present invention relates to a kind of kit and preparation method thereof, and in particular to a kind of antistreptolysin O (ASO) detection reagent
Box and preparation method thereof.
Background technology
Immunoturbidimetry (Turbidimetric inhibition immuno assay) is that antigen-antibody combines dynamic survey
Determine method.Its general principle is：When antigen and antibody react and suitable (the general provision antibody of ratio in special dilution system
It is excessive) when, in the presence of the poly- agent of rush (polyethylene glycol etc.) of the soluble immune complex of formation in dilution system, from liquid phase
Separate out, form particulate, reaction solution turbidity is occurred.When antibody concentration is fixed, the amount of the immune complex of formation is with sample
The increase of middle amount of antigen and increase, the turbidity of reaction solution is consequently increased.By the turbidity and series of standards that determine reaction solution
Product are compareed, you can calculate the content of antigen in sample.
Latex enhancing immune turbidimetry is that the corresponding antibody/antigen of test substance is coated on into a diameter of nano rubber latex
On particle, make antigen-antibody conjugate formation network structure, light is after, and the Strength Changes of transmitted light and scattered light are more aobvious
Write, so as to improve the sensitiveness of experiment.
The patent of Beijing Strong Biotechnologies, Inc. discloses the method for being related to latex sensitization and reagent (application
Number 201010615283.2), specifically, the method for the latex sensitization invented is first passes through chemical bond by latex nothing to do with egg
It is white to combine, glutaraldehyde is reused by antigen or antibody linked on unrelated protein, so that latex sensitization.The preparation of reagent two
Journey, which needs to centrifuge twice, changes liquid, is unfavorable for preparation of industrialization.
The patent of Ningbo Meikang Biotechnology Co., Ltd. discloses a kind of detection of antistreptolysin O (ASO) antibody
Kit (application number 201410804871.9).Its described albumen latex particle composites is that combined protein is marked at polystyrene
The compound formed on latex particle；The combined protein is formed by streptolysin O and inert protein by chemical crosslinking.
The preparation process of reagent two needs a ultrafiltration, and liquid is changed in centrifugation twice, is wasted time and energy.
The side for being related to coupling protein matter on a kind of carboxylated polystyrene latex particles with spacerarm is invented by Medical University Of Tianjin
Method (application number 201210005525.5).Its described will be coupled when the latex microsphere of antibody is separated with remaining antibody employs
Tangential flow filtration technology, overcomes the shortcoming easy to fall off and inactivation of antibody in centrifugal separation process.
To sum up, excess component is removed, change buffer solution in order to reach in preparation method of the prior art, change the mesh such as pH value
Be required to centrifuged or ultrafiltration step, cause existing preparation method to waste time and energy, be unfavorable for industrialization.Moreover, existing skill
Inert protein or unrelated protein are introduced in the file that art is recorded, the process of the introducing inert protein or unrelated protein is cumbersome, and
Success rate is low, efficiency is low and time-consuming.
The content of the invention
The technical problems to be solved by the invention are：The step of prior art is required to centrifugation or ultrafiltration, wastes time and energy,
It is unfavorable for industrialized problem, it is therefore intended that there is provided a kind of antistreptolysin O (ASO) detection kit and preparation method thereof, its
By the optimization of the scope that feeds intake of each component, the step of removing excess component without centrifugation, ultrafiltration etc. can be effectively reached, makes sensitization
Latex afterwards participates in reacting directly as reagent two, and substantially increasing conventional latex strengthens the preparation efficiency of immunoturbidimetry reagent,
Avoid the purge processes such as the centrifugation taken time and effort, ultrafiltration.
The present invention is achieved through the following technical solutions：
A kind of antistreptolysin O (ASO) detection kit, including reagent two, the reagent two be using latex, buffer solution,
After EDC and HEPES hybrid reactions, NaOH regulation pH to 7.00~8.00 are added, SLO, sealer I and closing is finally added
The finished product being made after agent II；The concentration of each material is in finished product：
The present invention is avoided that the introducing of inert protein or unrelated protein by the optimization of said components, greatly simplifies behaviour
Make step.Also, the present invention is based on multiple experiments, and then determines the scope that feeds intake of each component in reagent two, by institute of the present invention
The scope that feeds intake set up is fed intake, and can remove centrifugation from or ultrafiltration removes the purpose of excess component, be highly suitable for industrialization
Production, improves production efficiency, and effect is more notable.
Further, the buffer solution is MES-NaOH solution, and the sealer I is Tween-20 solution, the sealer
II is BSA solution.Further, the concentration of the MES-NaOH solution is 25~50mM, and pH value is 6.00；The Tween-20
The concentration of solution is 20%；The concentration of the BSA solution is 10%.
Being preferably provided with by the scope of the present invention value of the invention, can effectively improve while preparation process is being simplified
Accuracy in detection.
Preferably, also include in the BSA solution 3% NaN3。NaN3Effect be anti-corrosion, be added into after reagent two
Final concentration is changed into 0.03%, still there is antisepsis.
Further, the latex is spaced arm latex.Streptolysin O molecular weight is about 69kd, and molecular weight is smaller,
After sensitization to latex, steric hindrance is formed, covers by the antibody recognition site in sample to be tested, is unfavorable for antigenic determinant
Exposure, is difficult to recognize that reaction efficiency is low by the antibody in sample to be tested.The present invention can use the latex of spaced arm simultaneously
Conventional latex is replaced, the set-up mode can effectively reduce the steric hindrance problem of antigen-antibody combination, be conducive to antigen to determine
The exposure of cluster, so as to be recognized rapidly by the antibody in sample to be tested, occurs specific agglutination, reaches preferable latex intensified
The effect that immunoturbidimetry is determined.
Percent concentrations of the present invention refer both to w/v concentration, i.e. mass/volume concentration, and unit is g/ml.Said components
Middle EDC is carbodiimide, and HEPES is 4- hydroxyethyl piperazineethanesulfonic acids, and SLO is streptolysin O, and MES is 2- morpholine second sulphurs
Acid, BSA is bovine serum albumin(BSA).
To ensure the accuracy weighed, the mode of EDC additions is：EDC is configured to the EDC that concentration is 0.7~1.2% molten
Liquid, the consumption of EDC solution is 7.50ml/L~7.70ml/L.Meanwhile, the concentration of heretofore described NaOH solution is preferably provided with
For 20%.
A kind of preparation method of antistreptolysin O (ASO) detection kit, wherein, the preparation method of reagent two includes：
Latex, buffer solution, EDC, HEPES, SLO, sealer I and sealer II are taken respectively；EDC solution is prepared, is prepared
NaOH solution；
Latex is added in buffer solution, EDC solution is added and is activated, add HEPES, adjusted with NaOH solution
PH, adds SLO and is chemically crosslinked, and then adds sealer I and sealer II and is closed, is eventually fabricated finished product.
Further, the time that the EDC solution is activated is 15min.The time of the chemical crosslinking is 2h.The envelope
The time closed is 0.5h.
The present invention compared with prior art, has the following advantages and advantages：
1st, control process of the present invention is very precisely strict, it is to avoid the impurity elimination such as centrifugation, ultrafiltration of conventional latex sensitization process
Process, substantially increasing conventional latex strengthens the preparation efficiency of immunoturbidimetry reagent, substantially increases production efficiency；
2nd, superior performance of the present invention, preparation process is very simple, easy, greatlys save time cost and human cost,
Overcome the shortcoming of prior art.
Embodiment
For the object, technical solutions and advantages of the present invention are more clearly understood, with reference to embodiment, to present invention work
Further to describe in detail, exemplary embodiment and its explanation of the invention is only used for explaining the present invention, is not intended as to this
The restriction of invention.
Embodiment 1
In a kind of antistreptolysin O (ASO) detection kit, including reagent one and reagent two, the present embodiment, the reagent two
In the concentration of each material be：
The latex of the reagent of this in the present embodiment two is preferably spaced arm latex.
Buffer solution is preferably the MES-NaOH solution that concentration is 25~50mM, and its preparation method is：Made after being dissolved using MES
Into the solution of 25~50mM concentration, MES-NaOH solution is then made using NaOH regulations pH to 6.0.Sealer I is preferably
Concentration is 20% Tween-20 solution.
Sealer II is preferably the BSA solution that concentration is 10%, also containing 3% NaN in the BSA solution3；Sealer II
Preparation process be：Weigh 10g BSA and 3g NaN3Afterwards, both are dissolved into water, and constant volume is that BSA is made to 100ml
Solution.
By taking 100ml as an example, the specific preparation method of the reagent two of the present embodiment is as follows：
NaOH is configured to the NaOH solution that concentration is 20%, EDC is configured to the EDC solution that concentration is 0.9%；Respectively
Measure latex 6ml, MES-NaOH solution 14ml, EDC solution 0.76ml, HEPES 0.5958g, SLO 7.6mg, Tween-20 molten
Liquid 2ml and BSA solution 1ml；HEPES is dissolved.
Latex is added in buffer solution, the activation that EDC solution carries out carboxyl is added, soak time is 15min, then add
Enter HEPES, pH to 7.60 is adjusted using NaOH solution, add SLO and be chemically crosslinked, the chemical crosslinking time is 2h, Ran Houjia
Enter sealer I and sealer II is closed, off-period is 0.5h, and last constant volume to 100ml is that finished product is made.
The reagent one of the present embodiment is the reagent one in existing antistreptolysin O (ASO) detection kit, in the present embodiment
There is provided the composition of one of which reagent one, concrete composition composition is as follows：
By taking 1000ml as an example, the specific preparation method of the reagent one of the present embodiment is as follows：
About 800ml purified waters are measured, weighs and dissolves HEPES 23.83g, pH value is adjusted to 7.00 with 20%NaOH, claims
Take and dissolve 9.00~15.00g of NaCl, measure and dissolve Tween-20 10.0ml, weigh and dissolve PEG6000 25.00~
33.00g, measures and dissolves preservative 0.30ml, measures and dissolves 10%BSA 10ml, last constant volume to 1000ml is to be made into
Product.
Embodiment 2
The present embodiment and the difference of embodiment 1 be, the amount of the preparation of reagent two in the present embodiment is different, the present embodiment with
Exemplified by 500ml, the specific preparation method of the reagent two of the present embodiment is as follows：
Latex 30ml, buffer solution 70ml, EDC 3.8ml, HEPES 2.9789g, SLO 38mg, sealer I are measured respectively
10ml and sealer II 5ml；EDC is configured to the EDC solution that concentration is 0.7~1.2%, HEPES is dissolved, will
NaOH is configured to the NaOH solution that concentration is 20%；
Latex is added in buffer solution, the activation that EDC solution carries out carboxyl is added, soak time is 15min, then add
Enter HEPES, pH to 7.40 is adjusted using NaOH solution, add SLO and be chemically crosslinked, the chemical crosslinking time is 2h, Ran Houjia
Enter sealer I and sealer II is closed, off-period is 0.5h, and last constant volume to 500ml is that finished product is made.
Embodiment 3
The present embodiment and the difference of embodiment 1 are that the preparation method of reagent two is different in the present embodiment, the present embodiment increase
The step of centrifugation, the specific setting of the present embodiment is as follows：
Latex 6ml, buffer solution 14ml, EDC 0.76ml, HEPES 0.5958g, SLO 7.6mg, sealer I are measured respectively
2ml and sealer II 1ml；；EDC is configured to the EDC solution that concentration is 0.7~1.2%；It is molten using NaOH after HEPES dissolvings
Liquid adjusts pH value to 7.50, obtains and redissolves liquid；
Latex is added in buffer solution, the activation that EDC solution carries out carboxyl is added, soak time is 15min,
18000r/min centrifuges 15min, adds the redissolution liquid that pH value is 7.50 and is redissolved, adds SLO and be chemically crosslinked, chemistry
Crosslinking time is 2h, 18000r/min centrifugation 15min, adds the redissolution liquid that pH value is 7.50 and is redissolved, then with then adding
Enter sealer I and sealer II is closed, off-period is 0.5h, and last constant volume to 100ml is that finished product is made.
The kit being made of above-described embodiment 1- embodiments 3 is detected that calibration data is as shown in table 1, third party
As shown in table 2, the testing result for doing clinical trial using 40 samples is as shown in table 3 for Quality Control test result.
Table 1
Table 2
Table 3
It can be proved by the data result of above-mentioned table 1- tables 3：The present invention without the impurity elimination process such as centrifugation, ultrafiltration, according to
So effectively testing result can be made very accurate.
Embodiment 4
The present embodiment and the difference of embodiment 1 are that the addition of each component is different in reagent two in the present embodiment, this reality
The concentration of each component in example is applied less than the present invention, the specific setting of the present embodiment is as follows：
Latex 5.6ml, buffer solution 14ml, EDC solution 0.73ml, HEPES 0.5958g, SLO 7.2mg, sealer I
2ml and sealer II 1ml；The pH value of NaOH regulation solution is 6.90.
Embodiment 5
The present embodiment and the difference of embodiment 1 are that the addition of each component is different in reagent two in the present embodiment, this reality
The specific setting for applying example is as follows：
Latex 5.9ml, buffer solution 14ml, EDC solution 0.755ml, HEPES 0.5958g, SLO 7.5mg, sealer I
2ml and sealer II 1ml；The pH value of NaOH regulation solution is 7.10.
Embodiment 6
The present embodiment and the difference of embodiment 1 are that the addition of each component is different in reagent two in the present embodiment, this reality
The specific setting for applying example is as follows：
Latex 6.1ml, buffer solution 14ml, EDC solution 0.765ml, HEPES 0.5958g, SLO 7.7mg, sealer I
2ml and sealer II 1ml；The pH value of NaOH regulation solution is 7.90.
Embodiment 7
The present embodiment and the difference of embodiment 1 are that the addition of each component is different in reagent two in the present embodiment, this reality
The concentration of each component in example is applied higher than the present invention, the specific setting of the present embodiment is as follows：
Latex 6.4ml, buffer solution 14ml, EDC solution 0.79ml, HEPES 0.5958g, SLO 8.0mg, sealer I
2ml and sealer II 1ml；The pH value of NaOH regulation solution is 8.10.
The kit being made of above-described embodiment 2 and embodiment 4- embodiments 7 is detected that third party's Quality Control test is tied
Fruit is as shown in table 4.
Table 4
, can be in letter by being preferably provided with for the scope of the present invention value by above-described embodiment 4-7 test data
While changing preparation process, accuracy in detection is effectively improved, effect is more notable.
Above-described embodiment, has been carried out further to the purpose of the present invention, technical scheme and beneficial effect
Describe in detail, should be understood that the embodiment that the foregoing is only the present invention, be not intended to limit the present invention
Protection domain, within the spirit and principles of the invention, any modification, equivalent substitution and improvements done etc. all should be included
Within protection scope of the present invention.
Claims (10)
1. a kind of antistreptolysin O (ASO) detection kit, including reagent two, it is characterised in that the reagent two be using latex,
After buffer solution, EDC and HEPES hybrid reactions, NaOH solution regulation pH to 7.00~8.00 is added, SLO, envelope is finally added
Close the finished product being made after agent I and sealer II；The concentration of each material is in finished product：
2. a kind of antistreptolysin O (ASO) detection kit according to claim 1, it is characterised in that the buffer solution
For MES-NaOH solution, the sealer I is Tween-20 solution, and the sealer II is BSA solution.
3. a kind of antistreptolysin O (ASO) detection kit according to claim 2, it is characterised in that the MES-
The concentration of NaOH solution is 25~50mM, and pH value is 6.00；The concentration of the Tween-20 solution is 20%；The BSA solution
Concentration be 10%.
4. a kind of antistreptolysin O (ASO) detection kit according to claim 3, it is characterised in that the BSA solution
In also include 3% NaN3。
5. a kind of antistreptolysin O (ASO) detection kit according to claim 1, it is characterised in that the latex is
Spaced arm latex.
6. a kind of antistreptolysin O (ASO) detection kit according to claim 1, it is characterised in that the NaOH is molten
The concentration of liquid is 20%；EDC is configured to the EDC solution that concentration is 0.7~1.2%, the consumption of EDC solution for 7.50ml/L~
7.70ml/L。
7. a kind of preparation method of antistreptolysin O (ASO) detection kit according to any one of claim 1~6, its
It is characterised by, the preparation method of reagent two includes：
Latex, buffer solution, EDC, HEPES, SLO, sealer I and sealer II are taken respectively；Prepare EDC solution respectively and NaOH is molten
Liquid；
By latex add buffer solution in, add EDC solution and activated, add HEPES, with NaOH solution regulation pH to
7.00~8.00, add SLO and be chemically crosslinked, then add sealer I and sealer II and closed, is eventually fabricated into
Product.
8. a kind of preparation method of antistreptolysin O (ASO) detection kit according to claim 7, it is characterised in that
The time that the EDC solution is activated is 15min.
9. a kind of preparation method of antistreptolysin O (ASO) detection kit according to claim 7, it is characterised in that
The time of the chemical crosslinking is 2h.
10. a kind of preparation method of antistreptolysin O (ASO) detection kit according to claim 7, it is characterised in that
The time of the closing is 0.5h.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108761089A (en) * | 2018-06-29 | 2018-11-06 | 迈克生物股份有限公司 | Preparation method for the reagent for detecting β2-microglobulin |
| CN108761090A (en) * | 2018-06-29 | 2018-11-06 | 迈克生物股份有限公司 | Preparation method for the reagent for detecting c reactive protein |
| CN108982827A (en) * | 2018-06-26 | 2018-12-11 | 浙江卓运生物科技有限公司 | A kind of latex immunoturbidimetry reagent |
| CN113311154A (en) * | 2021-05-28 | 2021-08-27 | 宁波瑞源生物科技有限公司 | Coupling method, diluent and application of immunomagnetic particles |
| CN114113578A (en) * | 2021-11-30 | 2022-03-01 | 湖南永和阳光生物科技股份有限公司 | Method for coupling fluorescent microspheres with antibody and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63298062A (en) * | 1987-05-28 | 1988-12-05 | Hitachi Chem Co Ltd | Determination of anti-streptolysin o antibody |
| CN1036078A (en) * | 1987-12-21 | 1989-10-04 | 艾博特公司 | The apparatus and method of chromatographic binding assay |
| CN101302526A (en) * | 2008-06-27 | 2008-11-12 | 四川省迈克科技有限责任公司 | Recombinant soluble streptococcus hemolyticus haemolysin O gene, recombinant protein and preparation thereof |
| CN102590524A (en) * | 2011-12-30 | 2012-07-18 | 北京九强生物技术股份有限公司 | Assay kit for neutrophil gelatinase-associated lipocalin |
| CN104198723A (en) * | 2014-08-11 | 2014-12-10 | 南京普朗医疗设备有限公司 | Rapid NGAL (Neutrophil Gelatinase Associated Lipocalin) detection kit based on amino acid spacer arm |
| CN104215754A (en) * | 2014-08-14 | 2014-12-17 | 上海睿康生物科技有限公司 | SLO protection eukaryotic expression, and kit containing eukaryotically expressed SLO protein |
| CN104897904A (en) * | 2015-05-29 | 2015-09-09 | 中生北控生物科技股份有限公司 | Method for marking protein by carboxyl microsphere |
-
2017
- 2017-07-06 CN CN201710547376.8A patent/CN107167586B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63298062A (en) * | 1987-05-28 | 1988-12-05 | Hitachi Chem Co Ltd | Determination of anti-streptolysin o antibody |
| CN1036078A (en) * | 1987-12-21 | 1989-10-04 | 艾博特公司 | The apparatus and method of chromatographic binding assay |
| CN101302526A (en) * | 2008-06-27 | 2008-11-12 | 四川省迈克科技有限责任公司 | Recombinant soluble streptococcus hemolyticus haemolysin O gene, recombinant protein and preparation thereof |
| CN102590524A (en) * | 2011-12-30 | 2012-07-18 | 北京九强生物技术股份有限公司 | Assay kit for neutrophil gelatinase-associated lipocalin |
| CN104198723A (en) * | 2014-08-11 | 2014-12-10 | 南京普朗医疗设备有限公司 | Rapid NGAL (Neutrophil Gelatinase Associated Lipocalin) detection kit based on amino acid spacer arm |
| CN104215754A (en) * | 2014-08-14 | 2014-12-17 | 上海睿康生物科技有限公司 | SLO protection eukaryotic expression, and kit containing eukaryotically expressed SLO protein |
| CN104897904A (en) * | 2015-05-29 | 2015-09-09 | 中生北控生物科技股份有限公司 | Method for marking protein by carboxyl microsphere |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108982827A (en) * | 2018-06-26 | 2018-12-11 | 浙江卓运生物科技有限公司 | A kind of latex immunoturbidimetry reagent |
| CN108761089A (en) * | 2018-06-29 | 2018-11-06 | 迈克生物股份有限公司 | Preparation method for the reagent for detecting β2-microglobulin |
| CN108761090A (en) * | 2018-06-29 | 2018-11-06 | 迈克生物股份有限公司 | Preparation method for the reagent for detecting c reactive protein |
| CN113311154A (en) * | 2021-05-28 | 2021-08-27 | 宁波瑞源生物科技有限公司 | Coupling method, diluent and application of immunomagnetic particles |
| CN114113578A (en) * | 2021-11-30 | 2022-03-01 | 湖南永和阳光生物科技股份有限公司 | Method for coupling fluorescent microspheres with antibody and application thereof |
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