CN106073795B - Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method and Sweat latent fingerprint process for show - Google Patents
Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method and Sweat latent fingerprint process for show Download PDFInfo
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- CN106073795B CN106073795B CN201610384933.4A CN201610384933A CN106073795B CN 106073795 B CN106073795 B CN 106073795B CN 201610384933 A CN201610384933 A CN 201610384933A CN 106073795 B CN106073795 B CN 106073795B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61B5/117—Identification of persons
- A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
- A61B5/1172—Identification of persons based on the shapes or appearances of their bodies or parts thereof using fingerprinting
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- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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Abstract
The fingerprint ridge shown existing for existing finger mark process for show is unintelligible, show reagent there are heavy metal ion, influences the problems such as health of operator in order to solve, and the present invention proposes a kind of gold nanoclusters Sweat latent fingerprint visualization reagent preparation method and Sweat latent fingerprint process for show.Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method of the present invention is using nanogold particle as seed, wrap up one layer of bovine serum albumin(BSA) BSA in surface, using BSA it is template in nanogold particle surface reduction gold nanoclusters in water phase, gold nanoclusters powder visualization reagent is obtained after separation, drying and grinding.Gold nanoclusters fluorescence quantum yield prepared by the present invention is high, and fluorescence duration time is long, has hypotoxicity;Finger mark shows operation rapidly and efficiently, can clearly show 30 days outmoded finger marks;Its performance for showing outmoded finger mark shows reagent better than tradition;Of the invention shows the higher fixed finger mark information of observing system energy, is conducive to test sensitivity.
Description
Technical field
The invention belongs to public security criminal technique finger marks to identify field, and in particular to a kind of gold nanoclusters Sweat latent fingerprint visualization reagent
Preparation method and Sweat latent fingerprint process for show.
Background technique
Fingerprint be it is coefficient by nature-nurture, so fingerprint everybody all have, but it is different.Due to hereditary object
The influence of many factors such as matter, skin texture, pathology and wound, the mastoid process streakline of fingerprint have specificity, diversity and
Stability, these characteristics is identify to the person and identification is provided by analysis to fingerprint ridge, identification, identification
Scientific basis.Simultaneously as having the characteristic of touching object trace, the finger mark being retained on object can really reflect the fingerprint for leaving people
Feature, latent is shown in criminal case, extracted, analyzed and is identified can effective asserting crime suspect's identity,
It is to aid in quick, the efficient approach of one kind of investigator's discovery, locking suspect.
Finger mark appearing technique, mainly by selecting corresponding method, (traditional method has luminescence method, solwution method, powder
Method, smoked aobvious method etc.), using sweat, grease, blood, the dirt etc. in the substance and finger mark that show finger mark occur association reaction or
It is irradiated using light, so that potential finger mark be made to be in the technology that coloured finger mark displays.With natural science field new technology
It continues to develop, finger mark, which shows to have become, integrates the special of the more natural science technologies such as physics, chemistry, biology, instrument analysis
Industry technology.In recent years, nano material attracts the pass of vast researcher with its special light, magnetic, electricity, sound and mechanical characteristic
Note, researcher start nano material technology being applied to showing for latent, and compared with common material, nano material is easier to
In conjunction with the inorganic, organic species in latent.And some fluorescent nano materials of tool are shone by light sources such as ultraviolet lights
Penetrating excitation can produce fluorescence, by detecting the fluorescence imaging that the nano material being incorporated on finger mark is issued to clearly anti-
Reflect fingerprint characteristic.Meanwhile nano material can also be combined with other materials, and nanocomposite is formed, can not only have
Effect ground shows difficulty, the complicated finger mark that crime point is retained on various objects, additionally it is possible to overcome and existing show that reagent is toxic to be had
Evil, there are the problems such as security risk for process for show.
It is applied to cadmium sulfide, the cadmium selenide, three oxidations that the fluorescent nano material that latent shows has molecule to coat at present
Two aluminium and Zinc oxide nanoparticle etc..But due to the poor fingerprint line for causing to show of the selective binding of finger mark residue
Line is unintelligible, contrast is unobvious, it is difficult to get a desired effect.And this kind of reagents are there are heavy metal ion, in synthesis and
During use, it is most likely that generate harm to the health of operator.
As it can be seen that existing finger mark process for show that there is the fingerprint ridges shown is unintelligible, show reagent there are heavy metal from
Son influences the problem of health of operator.
Summary of the invention
In order to solve, the fingerprint ridge shown existing for existing finger mark process for show is unintelligible, showing reagent, there are heavy metals
Ion influences the problems such as health of operator, and the present invention proposes a kind of gold nanoclusters Sweat latent fingerprint visualization reagent preparation side
Method and Sweat latent fingerprint process for show.
Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method of the present invention, using nanogold particle as seed, surface wraps up one layer
Bovine serum albumin(BSA) BSA using BSA is template in nanogold particle surface reduction gold nanoclusters in water phase, through separation, drying and
Gold nanoclusters powder visualization reagent is obtained after grinding.
Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method of the present invention, which comprises the following steps:
S101, by 48ml ultrapure water and 1ml mass fraction be 1% aqueous solution of chloraurate after mixing, in electric heating cover
Middle heating stirring is rapidly added the trisodium citrate aqueous solution that 1ml mass fraction is 5% to boiling with vigorous stirring;Keep molten
Liquid boils and is vigorously stirred 2 min, and solution colour becomes claret;Stop heating, is further continued for being vigorously stirred until solution is cooling
To room temperature, nanogold particle solution is obtained, puts it into 4 DEG C of refrigerator and saves backup;
S102, nano-Au solution 10ml in step S101 is taken, the bovine serum albumin(BSA) that concentration is 20-60mg/mL is added
(BSA) solution 3-10ml stands 10-20h after mixing, obtains the nanogold particle solution of BSA package;
The chlorauric acid solution that 5ml concentration is 5-18mmol/L is added drop-wise to step S102 by S103, under agitation dropwise
In the nanogold particle solution of the BSA package of preparation, 2min is reacted, the NaOH solution that 0.5mL concentration is 1mol/L is added, then
It is put into continuous ultrasound 5-10h in Ultrasound Instrument, obtains the gold nanoclusters solution being reduced directly as seed on its surface using nanogold;
S104, the pH value of gold nanoclusters solution prepared by step S103 is adjusted to 4.6-5.8, is centrifugated, will precipitates
10-18h is dried in vacuo after washing at 37 DEG C, finally by the grind into powder in the agate mortar of the solid after drying, obtain with
Nanogold is the gold nanoclusters Sweat latent fingerprint visualization reagent powder that seed is reduced directly on its surface.
Preferably, the step S102 are as follows: take the nano-Au solution 10ml in step S101, addition concentration is 30-50mg/
The BSA solution 5-8ml of mL, stands 16h after mixing, obtains the nanogold particle solution of BSA package;
The step S103 are as follows: under agitation, the chlorauric acid solution that 5ml concentration is 10-12mmol/L is dripped dropwise
It is added in the nanogold particle solution of BSA package of step S102 preparation, reacts 2min, it is 1mol/L's that 0.5mL concentration, which is added,
NaOH solution is subsequently placed into continuous ultrasound 6-8h in Ultrasound Instrument, obtains the gold being reduced directly as seed on its surface using nanogold
Nano-cluster solution;
The step S104 are as follows: the pH value of gold nanoclusters solution prepared by step S103 is adjusted to 5.2, is centrifugated,
12-15h will be dried in vacuo after washing of precipitate at 37 DEG C, finally by the grind into powder in the agate mortar of the solid after drying,
Obtain the gold nanoclusters Sweat latent fingerprint visualization reagent powder being reduced directly as seed on its surface using nanogold.
A kind of Sweat latent fingerprint process for show, using the gold nanoclusters Sweat latent fingerprint visualization reagent of the method for the present invention preparation to outmoded
Sweat latent fingerprint is shown, comprising the following steps:
S201, reagent preparation, prepare gold nanoclusters Sweat latent fingerprint visualization reagent powder using the method for the present invention;
S202, finger mark is restrained, volunteer restrains finger mark on object surface, makes finger mark sample;
S203, aobvious finger mark is brushed, the gold nanoclusters sweat of the aobvious step S201 preparation of brush is latent on the finger mark sample of step S202 refers to
Print visualization reagent powder;
S204, finger mark observation are fixed, and in the case where showing observing system, the Sweat latent fingerprint figure of aobvious finger mark step S203 is brushed in observation
Picture.
Further, the step S202 are as follows: volunteer soaps both hands dry only after, with thumb contact forehead and
Then finger mark is restrained in wing of nose two sides on glass, coin, cortex notebook or experimental bench surface, obtain clearly finger mark sample.
Further, the resting period is 7-30 days to the finger mark sample in the step S202 in its natural state.
Further, the step S203 are as follows: it is aobvious to pick Sweat latent fingerprint prepared by a small amount of step S201 with soft brush
Existing agent powder is gently brushed along the direction of finger mark mastoid process streakline and is shown, displays streakline.
Further, it is to utilize alternative light source, colour light filter mirror, digital list that observing system is shown in the step S204
What reverse phase machine was built shows observing system.
Further, the step S204 finger mark observation is fixed, including, in darkroom, shown with alternative light source irradiation
The Sweat latent fingerprint that reagent brush shows, the fluorescence that finger mark generates penetrate colour light filter mirror, are recorded by digital single-lens reflex camera, by finger mark cream
Lug line and its minutia are fixed up.
The present invention is based on the controllability of nano Au particle size and good biocompatibilities, utilize bovine serum albumin(BSA)
Nanogold and gold nanoclusters are combined, the regulation of size can also be realized while making it have fluorescence, with this
Prepare novel fluorescence visualization reagent, in gold nanoclusters solution, the partial size of gold nanoclusters is 10-20nm, and to Sweat latent fingerprint into
Clearly finger mark is presented under alternative light source detection in row fluorescent marker.
The present invention has the advantage that
1. the gold nanoclusters fluorescence quantum yield of preparation is high, and fluorescence duration time is long, has hypotoxicity;
2. finger mark shows operation rapidly and efficiently, it can clearly show 30 days outmoded finger marks;
3. the performance that gold nanoclusters prepared by the present invention show outmoded finger mark shows reagent better than tradition;
4. showing the higher fixed finger mark information of observing system energy, be conducive to test sensitivity.
Detailed description of the invention
Fig. 1 is that latent shows effect picture on different object surfaces.(a. glass b. coin c. experimental bench d. cortex pen
Remember this surface)
Fig. 2 is to show observing system and show schematic illustration.
Specific embodiment
Embodiment 1
A kind of production method of novel Sweat latent fingerprint visualization reagent, which comprises the following steps:
(1) after mixing by 48ml ultrapure water and 1% aqueous solution of chloraurate of 1ml, heating stirring is extremely in electric heating cover
Boiling, is vigorously stirred down and is rapidly added 5% trisodium citrate aqueous solution of 1ml.It keeps solution boiling and is vigorously stirred 2 min, it is molten
Liquid color becomes claret.Stop heating, be further continued for being vigorously stirred until solution be cooled to room temperature, obtain nano-Au solution, will
It is saved backup in its refrigerator for being put into 4 DEG C;
(2) the nano-Au solution 10ml in step (1) is taken, the BSA solution 10ml that concentration is 20mg/mL is added, mixing is equal
10h is stood after even, obtains the nanogold particle of BSA package;
(3) under agitation, the chlorauric acid solution that 5ml concentration is 5mmol/L is added drop-wise to dropwise in step (2)
In the nano-Au solution of BSA package, 2min is reacted, the NaOH solution of 0.5mL 1mol/L is added, is subsequently placed into Ultrasound Instrument and connects
Continuous ultrasound 10h, obtains the gold nanoclusters solution being reduced directly as seed on its surface using nanogold, through detecting, gained gold nano
The partial size of cluster is 12-20nm;
(4) pH value of gold nanoclusters solution prepared by step (3) is adjusted to 5.8, centrifuge separation, by after washing of precipitate
It is dried in vacuo 10h at 37 DEG C, finally by the grind into powder in the agate mortar of the solid after drying, obtains using nanogold as seed
In the gold nanoclusters powder of its surface direct-reduction.
Embodiment 2
A kind of production method of novel Sweat latent fingerprint visualization reagent, which comprises the following steps:
(1) after mixing by 48ml ultrapure water and 1% aqueous solution of chloraurate of 1ml, heating stirring is extremely in electric heating cover
Boiling, is vigorously stirred down and is rapidly added 5% trisodium citrate aqueous solution of 1ml.It keeps solution boiling and is vigorously stirred 2 min, it is molten
Liquid color becomes claret.Stop heating, be further continued for being vigorously stirred until solution be cooled to room temperature, obtain nano-Au solution, will
It is saved backup in its refrigerator for being put into 4 DEG C;
(2) the nano-Au solution 10ml in step (1) is taken, the BSA solution 3ml that concentration is 60mg/mL is added, mixing is equal
20h is stood after even, obtains the nanogold particle of BSA package;
(3) under agitation, the HAuCl4 solution that 5ml concentration is 18mmol/L is added drop-wise to dropwise in step (2)
In the nano-Au solution of BSA package, 2min is reacted, the NaOH solution of 0.5mL 1mol/L is added, is subsequently placed into Ultrasound Instrument and connects
Continuous ultrasound 5h, obtains the gold nanoclusters solution being reduced directly as seed on its surface using nanogold, through detecting, gained gold nanoclusters
Partial size be 10-15nm;
(4) pH value of gold nanoclusters solution prepared by step (3) is adjusted to 4.6, centrifuge separation, by after washing of precipitate
It is dried in vacuo 18h at 37 DEG C, finally by the grind into powder in the agate mortar of the solid after drying, obtains using nanogold as seed
In the gold nanoclusters powder of its surface direct-reduction.
Embodiment 3
A kind of production method of novel Sweat latent fingerprint visualization reagent, which comprises the following steps:
(1) after mixing by 48ml ultrapure water and 1% aqueous solution of chloraurate of 1ml, the heating stirring in electric heating cover
To boiling, it is vigorously stirred down and is rapidly added 5% trisodium citrate aqueous solution of 1ml.It keeps solution boiling and is vigorously stirred 2 min,
Solution colour becomes claret.Stop heating, be further continued for being vigorously stirred until solution be cooled to room temperature, obtain nano-Au solution,
It puts it into 4 DEG C of refrigerator and saves backup;
(2) the nano-Au solution 10ml in step (1) is taken, the BSA solution 8ml that concentration is 30mg/mL is added, mixing is equal
16h is stood after even, obtains the nanogold particle of BSA package;
(3) under agitation, the HAuCl4 solution that 5ml concentration is 10mmol/L is added drop-wise to dropwise in step (2)
In the nano-Au solution of BSA package, 2min is reacted, the NaOH solution of 0.5mL 1mol/L is added, is subsequently placed into Ultrasound Instrument and connects
Continuous ultrasound 8h, obtains the gold nanoclusters solution being reduced directly as seed on its surface using nanogold, through detecting, gained gold nanoclusters
Partial size be 11-16nm;
(4) pH value of gold nanoclusters solution prepared by step (3) is adjusted to 5.2, centrifuge separation, after washing of precipitate
12h is dried in vacuo at 37 DEG C, finally by the grind into powder in the agate mortar of the solid after drying, obtain with nanogold for kind
The gold nanoclusters powder that son is reduced directly on its surface.
Embodiment 4
A kind of production method of novel Sweat latent fingerprint visualization reagent, which comprises the following steps:
(1) after mixing by 48ml ultrapure water and 1% aqueous solution of chloraurate of 1ml, the heating stirring in electric heating cover
To boiling, it is vigorously stirred down and is rapidly added 5% trisodium citrate aqueous solution of 1ml.It keeps solution boiling and is vigorously stirred 2 min,
Solution colour becomes claret.Stop heating, be further continued for being vigorously stirred until solution be cooled to room temperature, obtain nano-Au solution,
It puts it into 4 DEG C of refrigerator and saves backup;
(2) the nano-Au solution 10ml in step (1) is taken, the BSA solution 5ml that concentration is 50mg/mL is added, mixing is equal
12h is stood after even, obtains the nanogold particle of BSA package;
(3) under agitation, the HAuCl4 solution that 5ml concentration is 12mmol/L is added drop-wise to dropwise in step (2)
In the nano-Au solution of BSA package, 2min is reacted, the NaOH solution of 0.5mL 1mol/L is added, is subsequently placed into Ultrasound Instrument and connects
Continuous ultrasound 6h, obtains the gold nanoclusters solution being reduced directly as seed on its surface using nanogold, through detecting, gained gold nanoclusters
Partial size be 12-19nm;
(4) pH value of gold nanoclusters solution prepared by step (3) is adjusted to 5.5, centrifuge separation, after washing of precipitate
15h is dried in vacuo at 37 DEG C, finally by the grind into powder in the agate mortar of the solid after drying, obtain with nanogold for kind
The gold nanoclusters powder that son is reduced directly on its surface.
Embodiment 5
A kind of novel Sweat latent fingerprint visualization reagent shows the method for outmoded Sweat latent fingerprint, comprising the following steps:
S1, novel Sweat latent fingerprint visualization reagent is prepared;
S2, volunteer soap both hands dry only after, with thumb contact forehead and wing of nose two sides, then glass,
Coin, cortex notebook restrain finger mark on experimental bench surface, obtain clearly finger mark sample, the finger mark sample is in nature
The lower resting period will be 7-30 days.
S3, Sweat latent fingerprint visualization reagent prepared by a small amount of step S1 is picked with soft brush, along finger mark mastoid process streakline
Direction, which is gently brushed, to be shown, and streakline is displayed.
S4, it is built using alternative light source, colour light filter mirror, digital single-lens reflex camera and shows that observing system is latent to fluorescence sweat to be referred to
Print carries out observation fixation, under dark condition, alternative light source is irradiated to the Sweat latent fingerprint shown by gold nanoclusters, finger mark generates
Fluorescence recorded by digital single-lens reflex camera through colour light filter mirror, finger mark mastoid process streakline and its minutia are fixed up.
The above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to preferred embodiment to this hair
It is bright to be described in detail, those skilled in the art should understand that, it can modify to technical solution of the present invention
Or equivalent replacement should all cover without departing from the objective and range of technical solution of the present invention in claim of the invention
In range.
Claims (9)
1. gold nanoclusters Sweat latent fingerprint visualization reagent preparation method, using nanogold particle as seed, it is pure that one layer of ox blood is wrapped up on surface
Albumen using bovine serum albumin(BSA) is template in nanogold particle surface reduction gold nanoclusters in water phase, through separation, drying and grinds
Gold nanoclusters powder visualization reagent is obtained after mill.
2. gold nanoclusters Sweat latent fingerprint visualization reagent preparation method according to claim 1, which is characterized in that including following step
It is rapid:
S101, by 48ml ultrapure water and 1ml mass fraction be 1% aqueous solution of chloraurate after mixing, in electric heating cover plus
Thermal agitation is rapidly added the trisodium citrate aqueous solution that 1ml mass fraction is 5% to boiling with vigorous stirring;Keep solution boiling
2 min are risen and are vigorously stirred, solution colour becomes claret;Stop heating, is further continued for being vigorously stirred until solution is cooled to room
Temperature obtains nano-Au solution, puts it into 4 DEG C of refrigerator and saves backup;
S102, nano-Au solution 10ml in step S101 is taken, the bovine serum albumin solution that concentration is 20-60mg/mL is added
3-10ml stands 10-20h after mixing, obtains the nanogold particle solution of bovine serum albumin(BSA) package;
The chlorauric acid solution that 5ml concentration is 5-18mmol/L is added drop-wise to step S102 preparation by S103, under agitation dropwise
Bovine serum albumin(BSA) package nanogold particle solution in, react 2min, be added 0.5mL concentration be 1mol/L NaOH it is molten
Liquid is subsequently placed into continuous ultrasound 5-10h in Ultrasound Instrument, obtains the gold nanoclusters being reduced directly as seed on its surface using nanogold
Solution;
S104, the pH value of gold nanoclusters solution prepared by step S103 is adjusted to 3-5.5, be centrifugated, after washing of precipitate
It is dried in vacuo 10-18h at 37 DEG C, finally by the grind into powder in the agate mortar of the solid after drying, obtains with nanogold
The gold nanoclusters Sweat latent fingerprint visualization reagent powder being reduced directly for seed on its surface.
3. gold nanoclusters Sweat latent fingerprint visualization reagent preparation method according to claim 2, which is characterized in that
The step S102 are as follows: take the nano-Au solution 10ml in step S101, the BSA solution that concentration is 30-50mg/mL is added
5-8ml stands 16h after mixing, obtains the nanogold particle solution of bovine serum albumin(BSA) package;
The step S103 are as follows: under agitation, the chlorauric acid solution that 5ml concentration is 10-12mmol/L is added drop-wise to dropwise
In the nanogold particle solution of the bovine serum albumin(BSA) package of step S102 preparation, 2min is reacted, 0.5mL concentration, which is added, is
The NaOH solution of 1mol/L is subsequently placed into continuous ultrasound 6-8h in Ultrasound Instrument, obtains direct on its surface by seed of nanogold
The gold nanoclusters solution of reduction;
The step S104 are as follows: the pH value of gold nanoclusters solution prepared by step S103 is adjusted to 4, centrifuge separation will precipitate
12-15h is dried in vacuo after washing at 37 DEG C, finally by the grind into powder in the agate mortar of the solid after drying, obtain with
Nanogold is the gold nanoclusters Sweat latent fingerprint visualization reagent powder that seed is reduced directly on its surface.
4. a kind of Sweat latent fingerprint process for show, which is characterized in that shown using finger mark described in any one of claims 1 to 3
Gold nanoclusters Sweat latent fingerprint visualization reagent prepared by agent preparation method shows outmoded Sweat latent fingerprint, comprising the following steps:
S201, reagent preparation, prepare gold nano using finger mark visualization reagent preparation method described in any one of claims 1 to 3
Cluster Sweat latent fingerprint visualization reagent powder;
S202, finger mark is restrained, volunteer restrains finger mark on object surface, makes finger mark sample;
S203, aobvious finger mark is brushed, the gold nanoclusters Sweat latent fingerprint that aobvious step S201 preparation is brushed on the finger mark sample of step S202 is aobvious
Existing agent powder;
S204, finger mark observation are fixed, and in the case where showing observing system, the Sweat latent fingerprint image of aobvious finger mark step S203 is brushed in observation.
5. Sweat latent fingerprint process for show according to claim 4, which is characterized in that the step S202 are as follows: volunteer will be double
Hand soaps dry only after, with thumb contact forehead and wing of nose two sides, then in glass, coin, cortex notebook or experiment
Finger mark is restrained on platform surface, obtains clearly finger mark sample.
6. Sweat latent fingerprint process for show according to claim 4, which is characterized in that the finger mark sample in the step S202 exists
The resting period is 7-30 days under natural conditions.
7. Sweat latent fingerprint process for show according to claim 4, which is characterized in that the step S203 are as follows: with soft brush
Son picks Sweat latent fingerprint visualization reagent powder prepared by a small amount of step S201, gently brushes and shows along the direction of finger mark mastoid process streakline, makes
Streakline displays.
8. Sweat latent fingerprint process for show according to claim 4, which is characterized in that show observing system in the step S204
Show observing system for what is built using alternative light source, colour light filter mirror, digital single-lens reflex camera.
9. Sweat latent fingerprint process for show according to claim 4, which is characterized in that the step S204 finger mark observation is fixed,
Including showing the Sweat latent fingerprint that reagent brush shows with alternative light source irradiation, the fluorescence that finger mark generates is through coloured in darkroom
Optical filtering is recorded by digital single-lens reflex camera, and finger mark mastoid process streakline and its minutia are fixed up.
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