CN106073795A - Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method and Sweat latent fingerprint process for show - Google Patents
Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method and Sweat latent fingerprint process for show Download PDFInfo
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- CN106073795A CN106073795A CN201610384933.4A CN201610384933A CN106073795A CN 106073795 A CN106073795 A CN 106073795A CN 201610384933 A CN201610384933 A CN 201610384933A CN 106073795 A CN106073795 A CN 106073795A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/117—Identification of persons
- A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
- A61B5/1172—Identification of persons based on the shapes or appearances of their bodies or parts thereof using fingerprinting
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- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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Abstract
In order to solve, the fingerprint ridge manifested that existing finger mark process for show exists is unintelligible, manifest reagent exists heavy metal ion, affect the problem such as healthy of operator, and the present invention proposes one gold nanoclusters Sweat latent fingerprint visualization reagent preparation method and Sweat latent fingerprint process for show.Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method of the present invention is with nanogold particle as seed, surface one layer of bovine serum albumin BSA of parcel, with BSA for template at nanogold particle surface reduction gold nanoclusters in aqueous phase, separated, be dried and grind after obtain gold nanoclusters powder visualization reagent.Gold nanoclusters fluorescence quantum yield prepared by the present invention is high, and fluorescence duration time is long, has hypotoxicity；Finger mark manifests operation rapidly and efficiently, can clearly manifest the outmoded finger mark of 30 days；Its performance manifesting outmoded finger mark is better than tradition and manifests reagent；The present invention manifests the fixing finger mark information that viewing system can be higher, beneficially test sensitivity.
Description
Technical field
The invention belongs to public security Forensic Science finger mark and identify field, be specifically related to a kind of gold nanoclusters Sweat latent fingerprint visualization reagent
Preparation method and Sweat latent fingerprint process for show.
Background technology
Fingerprint is coefficient by nature-nurture, thus fingerprint everybody all have, but different.Due to heredity thing
The impact of the many factors such as matter, skin texture, pathology and wound, the mastoid process streakline of fingerprint have specificity, multiformity and
Stability, these characteristics be by the analysis of fingerprint ridge, identify, identify thus the person is differentiated and identification provides
Scientific basis.Simultaneously as have the characteristic of tactile thing trace, the finger mark being retained on object can truly reflect the fingerprint leaving over people
Feature, in criminal case, latent manifested, extract, analyze and identifies can effective asserting crime suspect identity,
It is to aid in the one approach quick, efficient that investigator finds, locks suspect.
Finger mark appearing technique, mainly by selecting corresponding method, (traditional method has luminescence method, solwution method, powder
Method, smoked aobvious method etc.), utilize the material manifesting finger mark and the perspiration in finger mark, oils and fats, blood, dirt etc. occur association reaction or
Light is utilized to irradiate, so that potential finger mark is the technology that coloured finger mark displays.Along with natural science field new technique
Development, finger mark manifests to become and integrates the special of the multi-door natural science technology such as physics, chemistry, biology, Instrumental Analysis
Industry technology.In recent years, nano material attracts the pass of vast researcher with its special light, magnetic, electricity, sound and mechanical characteristic
Note, research worker starts to be applied to nano material technology manifesting of latent, and compared with common material, nano material is easier to
Inorganic, organic species in latent are combined.And some are had epipolic nano material and are shone by light sources such as ultraviolet lights
Penetrate to excite and can produce fluorescence, fluorescence imaging that the nano material being combined on finger mark by detection is sent thus the most anti-
Reflect fingerprint characteristic.Meanwhile, nano material can also combine with other materials, and forms nano composite material, has been possible not only to
Effect ground manifests crime and divides the difficulty being retained on various object, complicated finger mark, additionally it is possible to overcomes and existing manifests that reagent is poisonous to be had
There is the difficult problems such as potential safety hazard in evil, process for show.
It is applied to cadmium sulfide that the fluorescent nano material that latent manifests has molecule to be coated with, cadmium selenide, three oxidations at present
Two aluminum and Zinc oxide nanoparticle etc..But, due to the fingerprint stricture of vagina that cause manifest poor with the selective binding of finger mark residue
Line is unintelligible, contrast is inconspicuous, it is difficult to get a desired effect.And there is heavy metal ion in this class reagent, synthesis and
During use, it is most likely that the healthy generation harm to operator.
Visible, existing finger mark process for show also exist that the fingerprint ridge manifested is unintelligible, manifest reagent and exist heavy metal from
Son, affect the healthy problem of operator.
Summary of the invention
In order to solve, the fingerprint ridge manifested of existing finger mark process for show existence is unintelligible, manifest reagent exists heavy metal
Ion, affecting the problem such as healthy of operator, the present invention proposes a kind of gold nanoclusters Sweat latent fingerprint visualization reagent preparation side
Method and Sweat latent fingerprint process for show.
Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method of the present invention, with nanogold particle as seed, surface wraps up one layer
Bovine serum albumin BSA, with BSA for template at nanogold particle surface reduction gold nanoclusters in aqueous phase, separated, be dried and
Gold nanoclusters powder visualization reagent is obtained after grinding.
Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method of the present invention, it is characterised in that comprise the following steps:
S101, by after 48ml ultra-pure water and aqueous solution of chloraurate mix homogeneously that 1ml mass fraction is 1%, add in electric heating cover
Thermal agitation, to boiling, is rapidly added the trisodium citrate aqueous solution that 1ml mass fraction is 5% with vigorous stirring；Keep solution boiling
Rising and be stirred vigorously 2 min, solution colour becomes claret；Stop heating, be further continued for being stirred vigorously until solution is cooled to room
Temperature, obtains nanogold particle solution, puts it in the refrigerator of 4 DEG C and saves backup；
S102, the nano-Au solution 10ml taken in step S101, add the bovine serum albumin (BSA) that concentration is 20-60mg/mL
Solution 3-10ml, stands 10-20h, obtains the nanogold particle solution of BSA parcel after mix homogeneously；
S103, under agitation, is dropwise added drop-wise to step S102 by the chlorauric acid solution that 5ml concentration is 5-18mmol/L and prepares
BSA parcel nanogold particle solution in, react 2min, add 0.5mL concentration be the NaOH solution of 1mol/L, be subsequently placed into
Continuous ultrasound 5-10h in Ultrasound Instrument, obtains the gold nanoclusters solution being reduced directly on its surface with nanometer gold for seed；
S104, the pH value of the gold nanoclusters solution prepared by step S103 being adjusted to 4.6-5.8, centrifugation, by washing of precipitate
After at 37 DEG C be vacuum dried 10-18h, finally by dried solid grind into powder in agate mortar, obtain with nanometer
The gold nanoclusters Sweat latent fingerprint visualization reagent powder that gold is reduced directly on its surface for seed.
Preferably, described step S102 is: take the nano-Au solution 10ml in step S101, and addition concentration is 30-50mg/
The BSA solution 5-8ml of mL, stands 16h, obtains the nanogold particle solution of BSA parcel after mix homogeneously；
Described step S103 is: under agitation, is dropwise added drop-wise to by the chlorauric acid solution that 5ml concentration is 10-12mmol/L
In the nanogold particle solution of BSA parcel prepared by step S102, reacting 2min, adding 0.5mL concentration is the NaOH of 1mol/L
Solution, is subsequently placed into continuous ultrasound 6-8h in Ultrasound Instrument, obtains the gold nano being reduced directly on its surface with nanometer gold for seed
Bunch solution；
Described step S104 is: the pH value of the gold nanoclusters solution prepared by step S103 is adjusted to 5.2, and centrifugation, by heavy
Form sediment at 37 DEG C, after washing, be vacuum dried 12-15h, finally by dried solid grind into powder in agate mortar, obtain
The gold nanoclusters Sweat latent fingerprint visualization reagent powder being reduced directly for seed on its surface with nanometer gold.
A kind of Sweat latent fingerprint process for show, uses gold nanoclusters Sweat latent fingerprint visualization reagent prepared by the inventive method to outmoded
Sweat latent fingerprint manifests, and comprises the following steps:
Prepared by S201, reagent, use the inventive method to prepare gold nanoclusters Sweat latent fingerprint visualization reagent powder；
S202, restraining finger mark, volunteer restrains finger mark on object surface, makes finger mark sample；
The aobvious finger mark of S203, brush, the gold nanoclusters Sweat latent fingerprint that on the finger mark sample of step S202 prepared by aobvious step S201 of brush shows
Existing agent powder；
S204, finger mark are observed fixing, under manifesting viewing system, observe the Sweat latent fingerprint image of aobvious finger mark step S203 of brush.
Further, described step S202 is: both hands are soaped after only drying by volunteer, with thumb contact forehead and
Wing of nose both sides, then restrain finger mark, it is thus achieved that finger mark sample clearly on glass, coin, cortex notebook or laboratory table surface.
Further, the finger mark sample resting period in its natural state in described step S202 is 7 30 days.
Further, described step S203 is: picks Sweat latent fingerprint prepared by a small amount of step S201 with soft brush and shows
Existing agent powder, the direction along finger mark mastoid process streakline is brushed aobvious gently, makes streakline display.
Further, described step S204 manifests viewing system for utilizing alternative light source, colour light filter mirror, digital list
What anti-phase machine was built manifests viewing system.
Further, described step S204 finger mark is observed fixing, including, in darkroom, irradiate with alternative light source and manifest
The Sweat latent fingerprint that reagent brush shows, the fluorescence that finger mark produces is through colour light filter mirror, by digital single-lens reflex camera record, by finger mark breast
Lug line and minutia thereof are fixed up.
Present invention controllability based on nano Au particle size and good biocompatibility, utilize bovine serum albumin
Nanometer gold and gold nanoclusters are combined so that it is the regulation and control of size can also be realized the while of having epipolic, with this
Preparing novel fluorescence visualization reagent, in gold nanoclusters solution, the particle diameter of gold nanoclusters is 10-20nm, and enters Sweat latent fingerprint
Row fluorescent labeling, under alternative light source detects, presents finger mark clearly.
Present invention have the advantage that
1. the gold nanoclusters fluorescence quantum yield of preparation is high, and fluorescence duration time is long, has hypotoxicity；
2. finger mark manifests operation rapidly and efficiently, can clearly manifest the outmoded finger mark of 30 days；
3. the gold nanoclusters that prepared by the present invention manifests the performance of outmoded finger mark and is better than tradition and manifests reagent；
4. manifest the fixing finger mark information that viewing system can be higher, beneficially test sensitivity.
Accompanying drawing explanation
Fig. 1 manifests design sketch for latent on different object surfaces.(a. glass b. coin c. laboratory table d. cortex pen
Remember this surface)
Fig. 2 is for manifesting viewing system and manifesting principle schematic.
Detailed description of the invention
Embodiment 1
The manufacture method of a kind of novel Sweat latent fingerprint visualization reagent, it is characterised in that comprise the following steps:
(1) by after 48ml ultra-pure water and 1ml 1% aqueous solution of chloraurate mix homogeneously, in electric heating cover, heated and stirred is to boiling
Rise, be stirred vigorously down and be rapidly added 1ml 5% trisodium citrate aqueous solution.Keep solution boiling and be stirred vigorously 2 min, solution
Color becomes claret.Stop heating, be further continued for being stirred vigorously until solution is cooled to room temperature, obtain nano-Au solution, by it
Put in the refrigerator of 4 DEG C and save backup；
(2) take the nano-Au solution 10ml in step (1), add the BSA solution 10ml that concentration is 20mg/mL, after mix homogeneously
Stand 10h, obtain the nanogold particle of BSA parcel；
(3) under agitation, the chlorauric acid solution that 5ml concentration is 5mmol/L is dropwise added drop-wise to the BSA bag in step (2)
In the nano-Au solution wrapped up in, react 2min, add the NaOH solution of 0.5mL 1mol/L, be subsequently placed into continuous ultrasound in Ultrasound Instrument
10h, obtains the gold nanoclusters solution being reduced directly on its surface with nanometer gold, after testing, the grain of gained gold nanoclusters for seed
Footpath is 12-20nm；
(4) pH value of the gold nanoclusters solution prepared by step (3) is adjusted to 5.8, centrifugation, by after washing of precipitate at 37 DEG C
Lower vacuum drying 10h, finally by dried solid grind into powder in agate mortar, obtains with nanometer gold for seed at it
The gold nanoclusters powder of surface direct-reduction.
Embodiment 2
The manufacture method of a kind of novel Sweat latent fingerprint visualization reagent, it is characterised in that comprise the following steps:
(1) by after 48ml ultra-pure water and 1ml 1% aqueous solution of chloraurate mix homogeneously, in electric heating cover, heated and stirred is to boiling
Rise, be stirred vigorously down and be rapidly added 1ml 5% trisodium citrate aqueous solution.Keep solution boiling and be stirred vigorously 2 min, solution
Color becomes claret.Stop heating, be further continued for being stirred vigorously until solution is cooled to room temperature, obtain nano-Au solution, by it
Put in the refrigerator of 4 DEG C and save backup；
(2) take the nano-Au solution 10ml in step (1), add the BSA solution 3ml that concentration is 60mg/mL, after mix homogeneously
Stand 20h, obtain the nanogold particle of BSA parcel；
(3) under agitation, the HAuCl4 solution that 5ml concentration is 18mmol/L is dropwise added drop-wise to the BSA bag in step (2)
In the nano-Au solution wrapped up in, react 2min, add the NaOH solution of 0.5mL 1mol/L, be subsequently placed into continuous ultrasound in Ultrasound Instrument
5h, obtains the gold nanoclusters solution being reduced directly on its surface with nanometer gold, after testing, the particle diameter of gained gold nanoclusters for seed
For 10-15nm；
(4) pH value of the gold nanoclusters solution prepared by step (3) is adjusted to 4.6, centrifugation, by after washing of precipitate at 37 DEG C
Lower vacuum drying 18h, finally by dried solid grind into powder in agate mortar, obtains with nanometer gold for seed at it
The gold nanoclusters powder of surface direct-reduction.
Embodiment 3
The manufacture method of a kind of novel Sweat latent fingerprint visualization reagent, it is characterised in that comprise the following steps:
(1) by after 48ml ultra-pure water and 1ml 1% aqueous solution of chloraurate mix homogeneously, in electric heating cover, heated and stirred is to boiling
Rise, be stirred vigorously down and be rapidly added 1ml 5% trisodium citrate aqueous solution.Keep solution boiling and be stirred vigorously 2 min, solution
Color becomes claret.Stop heating, be further continued for being stirred vigorously until solution is cooled to room temperature, obtain nano-Au solution, by it
Put in the refrigerator of 4 DEG C and save backup；
(2) take the nano-Au solution 10ml in step (1), add the BSA solution 8ml that concentration is 30mg/mL, after mix homogeneously
Stand 16h, obtain the nanogold particle of BSA parcel；
(3) under agitation, the HAuCl4 solution that 5ml concentration is 10mmol/L is dropwise added drop-wise to the BSA bag in step (2)
In the nano-Au solution wrapped up in, react 2min, add the NaOH solution of 0.5mL 1mol/L, be subsequently placed into continuous ultrasound in Ultrasound Instrument
8h, obtains the gold nanoclusters solution being reduced directly on its surface with nanometer gold, after testing, the particle diameter of gained gold nanoclusters for seed
For 11-16nm；
(4) pH value of the gold nanoclusters solution prepared by step (3) is adjusted to 5.2, centrifugation, by after washing of precipitate 37
It is vacuum dried 12h at DEG C, finally by dried solid grind into powder in agate mortar, obtains existing for seed with nanometer gold
The gold nanoclusters powder of its surface direct-reduction.
Embodiment 4
The manufacture method of a kind of novel Sweat latent fingerprint visualization reagent, it is characterised in that comprise the following steps:
(1) by after 48ml ultra-pure water and 1ml 1% aqueous solution of chloraurate mix homogeneously, in electric heating cover, heated and stirred is to boiling
Rise, be stirred vigorously down and be rapidly added 1ml 5% trisodium citrate aqueous solution.Keep solution boiling and be stirred vigorously 2 min, solution
Color becomes claret.Stop heating, be further continued for being stirred vigorously until solution is cooled to room temperature, obtain nano-Au solution, by it
Put in the refrigerator of 4 DEG C and save backup；
(2) take the nano-Au solution 10ml in step (1), add the BSA solution 5ml that concentration is 50mg/mL, after mix homogeneously
Stand 12h, obtain the nanogold particle of BSA parcel；
(3) under agitation, the HAuCl4 solution that 5ml concentration is 12mmol/L is dropwise added drop-wise to the BSA bag in step (2)
In the nano-Au solution wrapped up in, react 2min, add the NaOH solution of 0.5mL 1mol/L, be subsequently placed into continuous ultrasound in Ultrasound Instrument
6h, obtains the gold nanoclusters solution being reduced directly on its surface with nanometer gold, after testing, the particle diameter of gained gold nanoclusters for seed
For 12-19nm；
(4) pH value of the gold nanoclusters solution prepared by step (3) is adjusted to 5.5, centrifugation, by after washing of precipitate 37
It is vacuum dried 15h at DEG C, finally by dried solid grind into powder in agate mortar, obtains existing for seed with nanometer gold
The gold nanoclusters powder of its surface direct-reduction.
Embodiment 5
A kind of method that novel Sweat latent fingerprint visualization reagent manifests outmoded Sweat latent fingerprint, comprises the following steps:
S1, prepare novel Sweat latent fingerprint visualization reagent；
Both hands are soaped after only drying by S2, volunteer, with thumb contact forehead and wing of nose both sides, then glass, coin,
Cortex notebook, laboratory table restrain finger mark on surface, it is thus achieved that finger mark sample clearly, and this finger mark sample is deposited in its natural state
Time is 7 30 days.
S3, pick Sweat latent fingerprint visualization reagent prepared by a small amount of step S1, along finger mark mastoid process streakline with soft brush
Direction is brushed aobvious gently, makes streakline display.
S4, utilize alternative light source, colour light filter mirror, digital single-lens reflex camera to build to manifest viewing system and refer to fluorescence antiperspirant is latent
Print carries out observing fixes, and under dark condition, alternative light source irradiates the Sweat latent fingerprint manifested by gold nanoclusters, and finger mark produces
Fluorescence through colour light filter mirror by digital single-lens reflex camera record, finger mark mastoid process streakline and minutia thereof are fixed up.
Above example is only in order to illustrate technical scheme and unrestricted, although with reference to preferred embodiment to this
Bright it is described in detail, it will be understood by those within the art that, technical scheme can be modified
Or equivalent, without deviating from objective and the scope of technical solution of the present invention, it all should contain the claim in the present invention
In the middle of scope.
Claims (9)
1. gold nanoclusters Sweat latent fingerprint visualization reagent preparation method, with nanogold particle as seed, surface one layer of Sanguis Bovis seu Bubali of parcel is pure
Albumen, with bovine serum albumin for template at nanogold particle surface reduction gold nanoclusters in aqueous phase, separated, be dried and grind
Gold nanoclusters powder visualization reagent is obtained after mill.
Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method the most according to claim 1, it is characterised in that include following step
Rapid:
S101, by after 48ml ultra-pure water and aqueous solution of chloraurate mix homogeneously that 1ml mass fraction is 1%, add in electric heating cover
Thermal agitation, to boiling, is rapidly added the trisodium citrate aqueous solution that 1ml mass fraction is 5% with vigorous stirring；Keep solution boiling
Rising and be stirred vigorously 2 min, solution colour becomes claret；Stop heating, be further continued for being stirred vigorously until solution is cooled to room
Temperature, obtains nano-Au solution, puts it in the refrigerator of 4 DEG C and saves backup；
S102, the nano-Au solution 10ml taken in step S101, adding concentration is the bovine serum albumin solution of 20-60mg/mL
3-10ml, stands 10-20h, obtains the nanogold particle solution of bovine serum albumin parcel after mix homogeneously；
S103, under agitation, is dropwise added drop-wise to step S102 by the chlorauric acid solution that 5ml concentration is 5-18mmol/L and prepares
Bovine serum albumin parcel nanogold particle solution in, react 2min, adding 0.5mL concentration is that the NaOH of 1mol/L is molten
Liquid, is subsequently placed into continuous ultrasound 5-10h in Ultrasound Instrument, obtains the gold nanoclusters being reduced directly on its surface with nanometer gold for seed
Solution；
S104, the pH value of the gold nanoclusters solution prepared by step S103 being adjusted to 3-5.5, centrifugation, after washing of precipitate
At 37 DEG C, it is vacuum dried 10-18h, finally by dried solid grind into powder in agate mortar, obtains with nanometer gold
The gold nanoclusters Sweat latent fingerprint visualization reagent powder being reduced directly on its surface for seed.
Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method the most according to claim 2, it is characterised in that
Described step S102 is: take the nano-Au solution 10ml in step S101, adds the BSA solution that concentration is 30-50mg/mL
5-8ml, stands 16h, obtains the nanogold particle solution of bovine serum albumin parcel after mix homogeneously；
Described step S103 is: under agitation, is dropwise added drop-wise to by the chlorauric acid solution that 5ml concentration is 10-12mmol/L
In the nanogold particle solution of bovine serum albumin parcel prepared by step S102, reacting 2min, adding 0.5mL concentration is
The NaOH solution of 1mol/L, is subsequently placed into continuous ultrasound 6-8h in Ultrasound Instrument, obtains with nanometer gold for seed direct on its surface
The gold nanoclusters solution of reduction；
Described step S104 is: the pH value of the gold nanoclusters solution prepared by step S103 is adjusted to 4, and centrifugation, by precipitation
After washing at 37 DEG C be vacuum dried 12-15h, finally by dried solid grind into powder in agate mortar, obtain with
Nanometer gold is the gold nanoclusters Sweat latent fingerprint visualization reagent powder that seed is reduced directly on its surface.
4. a Sweat latent fingerprint process for show, it is characterised in that use the finger mark according to any one of claims 1 to 3 to manifest
Outmoded Sweat latent fingerprint is manifested by the gold nanoclusters Sweat latent fingerprint visualization reagent prepared by agent preparation method, comprises the following steps:
Prepared by S201, reagent, use the inventive method to prepare gold nanoclusters Sweat latent fingerprint visualization reagent powder；
S202, restraining finger mark, volunteer restrains finger mark on object surface, makes finger mark sample；
The aobvious finger mark of S203, brush, the gold nanoclusters Sweat latent fingerprint that on the finger mark sample of step S202 prepared by aobvious step S201 of brush shows
Existing agent powder；
S204, finger mark are observed fixing, under manifesting viewing system, observe the Sweat latent fingerprint image of aobvious finger mark step S203 of brush.
Sweat latent fingerprint process for show the most according to claim 4, it is characterised in that described step S202 is: volunteer will be double
Hands soaps after only drying, with thumb contact forehead and wing of nose both sides, then in glass, coin, cortex notebook or experiment
Platform restrains finger mark on surface, it is thus achieved that finger mark sample clearly.
Sweat latent fingerprint process for show the most according to claim 4, it is characterised in that the finger mark sample in described step S202 exists
The naturalness lower resting period is 7 30 days.
Sweat latent fingerprint process for show the most according to claim 4, it is characterised in that described step S203 is: with soft brush
Son picks Sweat latent fingerprint visualization reagent powder prepared by a small amount of step S201, and the direction along finger mark mastoid process streakline is brushed aobvious gently, makes
Streakline displays.
Sweat latent fingerprint process for show the most according to claim 4, it is characterised in that manifest viewing system in described step S204
For utilizing what alternative light source, colour light filter mirror, digital single-lens reflex camera built to manifest viewing system.
Sweat latent fingerprint process for show the most according to claim 4, it is characterised in that described step S204 finger mark is observed fixing,
Including, in darkroom, irradiating with alternative light source and manifest the Sweat latent fingerprint that reagent brush shows, the fluorescence that finger mark produces is through coloured
Filter lens, by digital single-lens reflex camera record, is fixed up finger mark mastoid process streakline and minutia thereof.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106995693A (en) * | 2017-04-01 | 2017-08-01 | 西南政法大学 | Show the method for finger mark using fluorescence magnetic noble-metal nanoclusters core-shell particles |
| CN109893138A (en) * | 2019-03-04 | 2019-06-18 | 西南政法大学 | It is a kind of visualize non-invasive diagnosis diabetes preparation and its preparation, application method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101268946A (en) * | 2008-04-30 | 2008-09-24 | 东北师范大学 | Method for latency fingerprint appearance of surface functionalization nano-gold particle |
| CN101446557A (en) * | 2008-12-29 | 2009-06-03 | 中国科学院长春应用化学研究所 | Method for strengthening latent fingerprint observation on non-permeating object by adopting gold nano-material |
| CN101485572A (en) * | 2009-02-24 | 2009-07-22 | 中国科学院长春应用化学研究所 | One-step method for developing latent finger prints using gold nanoparticles |
| CN101703404A (en) * | 2009-08-28 | 2010-05-12 | 东北师范大学 | Method for showing fingerprints on various object surfaces and keeping DNA information |
| CN103431867A (en) * | 2013-08-20 | 2013-12-11 | 北京科技大学 | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein |
-
2016
- 2016-06-02 CN CN201610384933.4A patent/CN106073795B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101268946A (en) * | 2008-04-30 | 2008-09-24 | 东北师范大学 | Method for latency fingerprint appearance of surface functionalization nano-gold particle |
| CN101446557A (en) * | 2008-12-29 | 2009-06-03 | 中国科学院长春应用化学研究所 | Method for strengthening latent fingerprint observation on non-permeating object by adopting gold nano-material |
| CN101485572A (en) * | 2009-02-24 | 2009-07-22 | 中国科学院长春应用化学研究所 | One-step method for developing latent finger prints using gold nanoparticles |
| CN101703404A (en) * | 2009-08-28 | 2010-05-12 | 东北师范大学 | Method for showing fingerprints on various object surfaces and keeping DNA information |
| CN103431867A (en) * | 2013-08-20 | 2013-12-11 | 北京科技大学 | Method for displaying latent fingerprints on fluorogold nano-cluster protected by protein |
Non-Patent Citations (1)
| Title |
|---|
| 喻彦林: "金纳米材料在潜在指纹显现中的应用", 《中国司法鉴定》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106995693A (en) * | 2017-04-01 | 2017-08-01 | 西南政法大学 | Show the method for finger mark using fluorescence magnetic noble-metal nanoclusters core-shell particles |
| CN106995693B (en) * | 2017-04-01 | 2020-03-06 | 西南政法大学 | Method for displaying fingerprint by using fluorescent magnetic precious metal nano cluster core-shell microspheres |
| CN109893138A (en) * | 2019-03-04 | 2019-06-18 | 西南政法大学 | It is a kind of visualize non-invasive diagnosis diabetes preparation and its preparation, application method |
| CN109893138B (en) * | 2019-03-04 | 2021-11-02 | 西南政法大学 | Preparation for visually diagnosing diabetes mellitus without wound, and preparation and use methods thereof |
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