CN101703404A - Method for showing fingerprints on various object surfaces and keeping DNA information - Google Patents
Method for showing fingerprints on various object surfaces and keeping DNA information Download PDFInfo
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- CN101703404A CN101703404A CN200910067469A CN200910067469A CN101703404A CN 101703404 A CN101703404 A CN 101703404A CN 200910067469 A CN200910067469 A CN 200910067469A CN 200910067469 A CN200910067469 A CN 200910067469A CN 101703404 A CN101703404 A CN 101703404A
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Abstract
The invention belongs to the technical field of detection, in particular to a method for showing fingerprints on various object surfaces and keeping DNA information. The method adopts metaliding, takes functionalized gold nanoparticles and silver nanoparticles as probes, and is combined with argentation to realize that the latent fingerprints and blood fingerprints on different object surfaces can be shown in one method. The gold and silver nanoparticles are adsorbed due to the action with the ingredients of the latent and blood fingerprints, silver ions in silver staining solution can be catalyzed to be reduced on the surfaces of the fingerprints, and the generated silver covers the surfaces of the gold nanoparticles, thus enhancing the detection signals. After the blood fingerprints are shown in the method, the DNA sequence can be further detected. In the invention, the reducing agent in the silver staining solution is further expanded, when vitamin C is taken as the reducing agent, the cost is lower, the reaction condition is wild, the operation is simpler, and further environmental protection can be realized. The method has high sensitivity and wide application range, and can show old fingerprints.
Description
Technical field
The invention belongs to the detection technique field, be specifically related to be applicable to that multiple object surface keeps latent antiperspirant, the blood fingerprint manifestation method of DNA information, this method still can be carried out the DNA sequence detection after manifesting fingerprint.
Background technology
Fingerprint plays important evidential function in forensic science and criminal suit process.More than 50 hundred million people have fingerprint separately in the world.Even finger skin peels off, the skin that newly grows, grain pattern are also constant as before.Therefore, fingerprint is solved a case becomes traditional criminal investigation method.Yet in all kinds of crime cases, the crime means that the offender adopts is more and more insidious, hidden more.The fingerprint that occurs in the scene of a crime often is potential, needs could observe its texture structure with some process for show.Therefore, the evaluation fingerprint is correctly found, extracts, manifested to science, for the investigation of case with punish crime, has important practice effect and immeasurable social value.
Laten fingerprints is present in various dissimilar object surfaces usually, mainly be divided into permeability object (as A4 paper) and impermeability object (as sheet glass, plastic bottle, copper sheet etc.), the laten fingerprints that manifests different objects surface usually need be used diverse ways and reagent.Laten fingerprints may be by staying behind perspiration, blood or the hemodilution, and wherein, the occult blood fingerprint can also detect DNA sequence except obtaining fingerprint image.Past, benzidine commonly used manifested the blood fingerprint, though this reagent sensitivity is higher, because of it is that strong carcinogen is eliminated.Develop after 1987 3,5,3 ', 5 '-tetramethyl benzidine becomes the substitute products of benzidine.Tetramethyl benzidine can become aeruginous with the occult blood fingerprint manifestation, is domestic the most frequently used blood fingerprint manifestation method.After tetramethyl benzidine manifests the blood fingerprint, when carrying out DNA detection again, DNA detection is not had influence, but blood flow volume is had certain requirement, containing the less occult blood fingerprint of blood often influences the DNA information that detects.Therefore, behind the occult blood fingerprint that manifests different objects surface, still can carry out the DNA sequence test smoothly and can guarantee to obtain more information, for confirming that crime provides favourable evidence.
The present invention has realized that not only a kind of reagent manifests the latent antiperspirant on different objects surface, occult blood fingerprint, does not destroy potential DNA information, can further carry out DNA sequence test, and can manifest the laten fingerprints that blood stays when extremely rare.
Summary of the invention
The objective of the invention is to realize that different objects (permeability object and impermeability object) do not destroy latent antiperspirant, the blood development of fingerprint on the surface of DNA composition.
The present invention adopts the gold, silver nano-particle of functionalization with being probe, utilizes metaliding, and latent antiperspirant, the blood fingerprint of realizing different objects (permeability object and impermeability object) surfaces are used with a kind of method and manifested.Behind the occult blood fingerprint manifestation, do the DNA sequence test again, can obtain composing entirely with the on all four DNA of spectrogram that directly obtains with blood testing.Metaliding is to utilize the substance reaction in the blood in the gold, silver nano-particle of functionalization and the occult blood fingerprint, utilizes argentation further to enlarge signal again, shows the occult blood fingerprint of different surfaces.This method can manifest the laten fingerprints that is printed behind diluted 400 times of the blood, and is highly sensitive, effective.
(1) many metalidings
Gold nano grain is respectively the gold grain of tryptophan, glutathione modification.
1, the preparation of the gold nano grain of tryptophan functionalization:
Aqueous solution of chloraurate and tryptophan aqueous solution stir, the amount of substance ratio is 9: 10, the politef reactor of partly mixed solution being packed into is put into baking oven and is heated to 50-100 ℃, sustained response 0.5-1 hour, behind the natural cooling, obtain the gold nano grain of tryptophan functionalization, particle diameter is 25-30nm.
Or under the room temperature, aqueous solution of chloraurate and tryptophan aqueous solution, the amount of substance ratio is 9: 10, slowly is heated to 50 ℃, obtains the gold nano grain of tryptophan functionalization, particle diameter is 25-30nm.The gold nano grain of tryptophan functionalization is easy to be precipitated out from solution as solid and preserves, and is distributed to again in the water with preceding, and is easy to use effective.
2, the preparation of the gold nano grain of glutathione functionalization:
Under the room temperature, 2.5-3mM aqueous solution of chloraurate and the glutathione aqueous solution of 1.9-2.5mM stir, volume ratio is 1: 7.8, regulate pH value in the 5-8 scope with the 1M/L sodium hydroxide, the sodium borohydride frozen water solution reduction that adds new preparation, continue to stir 10-12 hour, obtain the gold nano grain of glutathione functionalization.
(2) monometallic sedimentation
Silver nano-grain is respectively the silver nano-grain of sodium citrate, aminoacid, sodium borohydride protection.
1, the preparation of the silver nano-grain of sodium citrate functionalization:
With the silver nitrate 100ml of 2.5mM, the sodium citrate 100ml of 2.5mM mixes stirring, adds the sodium borohydride frozen water solution of the 6ml5mM of new preparation, can obtain xanchromatic colloid solution, continues stirring reaction 30 minutes, and standing over night just obtains silver nano-grain.
2, the preparation of the silver nano-grain of cysteine functionalization:
The aqueous solution of silver nitrate is under agitation dropwise added the new sodium borohydride frozen water solution of preparing, can obtain xanchromatic colloid solution, the aqueous solution that adds cysteine then stirs, and continues stirring reaction 30 minutes, and standing over night just obtains silver nano-grain.
(3) process for show:
The sample such as A4 paper, sheet glass, copper sheet that is loaded with laten fingerprints was immersed above-mentioned gold, silver nanoparticles solution after 5-10 minute, the sample taking-up is washed with tap water, wash off fail with fingerprint in composition reaction nanometer gold, silver granule, immersing the silver for preparing then dyes in the solution, sample soaks after 5-15 minute and takes out, with drying after the tap water flushing, promptly obtain fingerprint image clearly.This method can be applied to permeability object A4 paper and impermeability object sheet glass, copper sheet etc. simultaneously.
Silver dyes the mixed solution that solution is hydroquinone and silver nitrate, hydroquinone is 1.5-4 with the amount of substance ratio of silver nitrate: 1, and the sodium citrate buffer that adds pH=3.5-3.8 is regulated pH value, and adding 2-3ml aqueous gelatin solution carries out sluggish, hydroquinone can reduce silver nitrate under the catalysis of Jenner's grain of rice as Reducing agent, and the argent that is reduced just is deposited on the goldc grains surface of fingerprint ridge absorption, manifest effect thereby strengthened, form clearly streakline.Reducing agent hydroquinone in the silver dye liquor can be replaced by vitamin C (ascorbic acid), the also available silver acetate of silver nitrate replaces, vitamin C is 5-12 with the amount of substance ratio of silver nitrate: 1, it is identical for manifesting effect to dye the time that manifests in the solution at silver, uses vitamin C as Reducing agent, need not regulate pH value, the reaction condition gentleness, operate simplyr, cost is lower than hydroquinone, and environmental protection more.
The present invention has following advantage:
1, method is simple, quick, easy to operate, highly sensitive, can be apparent in the laten fingerprints on permeability object and impermeability object surface, can realize manifesting of outmoded laten fingerprints.
2, not by instrument (bore hole observation) can discern and manifest laten fingerprints; All obtain manifests fingerprint and can directly take pictures under natural light and fetch data.
3, the present invention easily prepares and preserves; Cost is low.
4, the present invention is green friendly, does not contain poisonous composition, and is safe in utilization, and the operator is had no side effect.
5, the present invention does not destroy the potential information in the fingerprint, manifest fingerprint with reagent provided by the invention and method after, still can carry out DNA tests.
Description of drawings
The latent antiperspirant fingerprint on the A4 paper surface that the gold nano grain of accompanying drawing 1, tryptophan functionalization manifests;
The occult blood fingerprint on the A4 paper surface that the gold nano grain of accompanying drawing 2, tryptophan functionalization manifests;
The latent antiperspirant fingerprint on the A4 paper surface that the silver nano-grain of accompanying drawing 3, sodium citrate functionalization manifests;
The occult blood fingerprint of seal behind diluted 400 times of the blood on the A4 paper surface that the silver nano-grain of accompanying drawing 4, sodium citrate functionalization manifests;
The latent antiperspirant fingerprint of the glass surface that the gold nano grain of accompanying drawing 5, tryptophan functionalization manifests;
The occult blood fingerprint of the glass surface that the gold nano grain of accompanying drawing 6, glutathione functionalization manifests;
The latent antiperspirant fingerprint on the copper sheet surface that the silver nano-grain of accompanying drawing 7, cysteine functionalization manifests;
The occult blood fingerprint of the glass surface that the silver nano-grain of accompanying drawing 8, cysteine functionalization manifests;
Complete the composing with direct the test from blood of the DNA that tests behind the fingerprint manifestation in accompanying drawing 9, the accompanying drawing 6 obtains the full comparison of composing of DNA.
The specific embodiment
Embodiment 1: the gold nano grain of tryptophan functionalization
Preparation: aqueous solution of chloraurate and tryptophan aqueous solution stir, the amount of substance ratio is 9: 10, the politef reactor of partly mixed solution being packed into is put into baking oven and is heated to 100 ℃, sustained response 0.5 hour, behind the natural cooling, obtain the gold nano grain of tryptophan functionalization, particle diameter is 25-30nm.
Manifest: the sample such as A4 paper, sheet glass, copper sheet that will be loaded with laten fingerprints immersed above-mentioned gold nano grain solution after 10 minutes, the sample taking-up is washed with tap water, wash off fail with fingerprint in composition reaction nanogold particle, immersing the silver for preparing then dyes in the solution, sample soaks after 5 minutes and takes out, with drying after the tap water flushing, promptly obtain fingerprint image clearly.(accompanying drawing 1,5)
Embodiment 2: the gold nano grain of tryptophan functionalization
Aqueous solution of chloraurate and tryptophan aqueous solution, the amount of substance ratio is 9: 10, slowly is heated to 50 ℃, obtains the gold nano grain of tryptophan functionalization, particle diameter is 25-30nm.
Manifest: the sample such as A4 paper, sheet glass, copper sheet that will be loaded with laten fingerprints immersed above-mentioned gold nano grain solution after 5 minutes, the sample taking-up is washed with tap water, wash off fail with fingerprint in composition reaction nanogold particle, immersing the silver for preparing then dyes in the solution, sample soaks after 10 minutes and takes out, with drying after the tap water flushing, promptly obtain fingerprint image clearly.(accompanying drawing 2)
Embodiment 3: the nanogold particle of glutathione functionalization
Preparation: under the room temperature, 2.5mM aqueous solution of chloraurate and the glutathione aqueous solution of 1.9mM stir, volume ratio is 1: 7.8, with 1M/L sodium hydrate regulator solution pH=8, the sodium borohydride frozen water solution reduction that adds new preparation, continue to stir 12 hours, obtain the gold nano grain of glutathione functionalization.
Manifest: the sample such as A4 paper, sheet glass, copper sheet that will be loaded with laten fingerprints immersed above-mentioned gold nano grain solution after 10 minutes, the sample taking-up is washed with tap water, wash off fail with fingerprint in composition reaction nanogold particle, immersing the silver for preparing then dyes in the solution, sample soaks after 10 minutes and takes out, with drying after the tap water flushing, promptly obtain fingerprint image clearly.(accompanying drawing 6)
Embodiment 4: the silver-colored granule of sodium citrate functionalization
Preparation: with the silver nitrate 100ml of 2.5mM, the sodium citrate 100ml of 2.5mM mixes stirring, adds the 6ml of new preparation, the sodium borohydride frozen water solution of 5mM can obtain xanchromatic colloid solution, continues stirring reaction 30 minutes, standing over night just obtains silver nano-grain.
Manifest: the sample such as A4 paper, sheet glass, copper sheet that will be loaded with laten fingerprints immersed above-mentioned silver nano-grain solution after 8 minutes, the sample taking-up is washed with tap water, wash off fail with fingerprint in composition reaction nano-Ag particles, immersing the silver for preparing then dyes in the solution, sample soaks after 8 minutes and takes out, with drying after the tap water flushing, promptly obtain fingerprint image clearly.(accompanying drawing 3,4)
Embodiment 5: the silver-colored granule of cysteine functionalization
Preparation: the sodium borohydride frozen water solution that the aqueous solution 100ml of 1mM silver nitrate is under agitation added the new preparation of 2ml 5mM, can obtain xanchromatic colloid solution, the aqueous solution that adds cysteine then stirs, continued stirring reaction 30 minutes, standing over night just obtains the silver nano-grain of cysteine functionalization.
Manifest: the sample such as A4 paper, sheet glass, copper sheet that will be loaded with laten fingerprints immersed above-mentioned silver nano-grain solution after 10 minutes, the sample taking-up is washed with tap water, wash off fail with fingerprint in composition reaction nano-Ag particles, immersing the silver for preparing then dyes in the solution, sample soaks after 15 minutes and takes out, with drying after the tap water flushing, promptly obtain fingerprint image clearly.(accompanying drawing 7,8)
Claims (6)
1. the method that manifests multiple object fingerprint surface and reservation DNA information, it is characterized in that: detector probe is the gold nano grain and the silver nano-grain of functionalization, manifest with metaliding, be respectively many metalidings and monometallic sedimentation, wherein gold nano grain is respectively the gold grain of tryptophan, glutathione modification, and silver nano-grain is respectively the silver-colored granule of sodium citrate, cysteine modified.
2. according to the described process for show of claim 1, it is characterized in that: will be loaded with the A4 paper of dive antiperspirant or occult blood fingerprint or impermeability sheet glass or copper sheet sample and immerse above-mentioned gold or silver nano-grain solution after 5-10 minute, the sample taking-up is washed with tap water, wash off fail with fingerprint in composition reaction nanometer gold, the silver granule, immersing the silver that has prepared then dyes in the solution, sample soaks after 5-15 minute and takes out, with drying after the tap water flushing, promptly obtain fingerprint image clearly, wherein silver dyes the mixed solution that solution is hydroquinone and silver nitrate, or be the mixed solution of vitamin C and silver nitrate, when the silver of use hydroquinone and silver nitrate dyes solution, hydroquinone is 1.5-4 with the amount of substance ratio of silver nitrate: 1, the citrate buffer solution that adds pH=3.5-3.8 is transferred pH, and adding the 2-3ml aqueous gelatin solution, vitamin C is 5-12 with the amount of substance ratio of silver nitrate: 1.
3. the preparation method of the gold nano grain of tryptophan functionalization, it is characterized in that: aqueous solution of chloraurate and tryptophan aqueous solution stir, the amount of substance ratio is 9: 10, the mixed solution politef reactor of packing into is put into baking oven and is heated to 50-100 ℃, sustained response 0.5-1 hour, behind the natural cooling, obtain the gold nano grain of tryptophan functionalization;
Or under the room temperature, aqueous solution of chloraurate and tryptophan aqueous solution stir, and the amount of substance ratio is 9: 10, slowly is heated to 50 ℃, obtains the gold nano grain of tryptophan functionalization.
4. the preparation method of the gold nano grain of glutathione functionalization, it is characterized in that: under the room temperature, 2.5-3mM aqueous solution of chloraurate and the glutathione aqueous solution of 1.9-2.5mM stir, volume ratio is 1: 7.8, regulate pH value in the 5-8 scope with the 1M/L sodium hydroxide, the sodium borohydride frozen water solution reduction that adds new preparation continues to stir 10-12 hour, obtains the gold nano grain of glutathione functionalization.
5. the preparation method of the silver nano-grain of sodium citrate functionalization, it is characterized in that: under the room temperature, the aqueous solution of silver nitrate and sodium citrate is stirred, the amount of substance ratio is 1: 1, dropwise add the sodium borohydride frozen water solution of the 5mM of new preparation, can obtain xanchromatic colloid solution, continued stirring reaction 30 minutes, standing over night just obtains silver nano-grain.
6. the preparation method of the silver nano-grain of cysteine functionalization, it is characterized in that: the sodium borohydride frozen water solution that the aqueous solution of silver nitrate is under agitation dropwise added new preparation, can obtain xanchromatic colloid solution, the aqueous solution that adds cysteine then stirs, continue to stir 30 minutes, standing over night just obtains silver nano-grain.
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| CN2009100674696A CN101703404B (en) | 2009-08-28 | 2009-08-28 | Method for showing fingerprints on various object surfaces and keeping DNA information |
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| CN102586411A (en) * | 2011-01-18 | 2012-07-18 | 温州安得森生物科技有限公司 | Safe and environment-friendly DNA (Deoxyribose Nucleic Acid) silver staining method for polyacrylamide gel |
| CN103070691A (en) * | 2012-11-09 | 2013-05-01 | 哈尔滨师范大学 | Method for manifesting latent fingerprints on basis of fluorescent gold nanoclusters |
| CN103356199A (en) * | 2013-07-19 | 2013-10-23 | 北京科技大学 | Method for manifesting metal surface hidden fingerprints through chemical sedimentation |
| CN103536296A (en) * | 2013-10-18 | 2014-01-29 | 浙江大学 | Method of detecting human sweat secretion |
| CN103536297A (en) * | 2013-10-18 | 2014-01-29 | 浙江大学 | Immune colloidal gold based latent fingerprint imaging method |
| CN103622701A (en) * | 2012-08-23 | 2014-03-12 | 武汉哇哇噻纳技术开发有限公司 | Method for showing fingerprints through silanization nanometer magnetic powder |
| CN106073795A (en) * | 2016-06-02 | 2016-11-09 | 西南政法大学 | Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method and Sweat latent fingerprint process for show |
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| US6306662B1 (en) * | 1999-02-04 | 2001-10-23 | Texas Tech University | Fingerprint development methods |
| US6592929B1 (en) * | 2000-07-13 | 2003-07-15 | Ladislav H. Berka | Method of exposing and developing a fingerprint from the surface of an oxidized metal object |
| CN101200005A (en) * | 2007-09-04 | 2008-06-18 | 广西师范大学 | Simple and fast preparing process for Au-Ag complex nanometer particles |
| CN100551328C (en) * | 2008-04-30 | 2009-10-21 | 东北师范大学 | Surface-functionalized nanogold particle is used for the latent fingerprint method for visualizing |
| CN101446557A (en) * | 2008-12-29 | 2009-06-03 | 中国科学院长春应用化学研究所 | Method for strengthening latent fingerprint observation on non-permeating object by adopting gold nano-material |
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| CN102586411A (en) * | 2011-01-18 | 2012-07-18 | 温州安得森生物科技有限公司 | Safe and environment-friendly DNA (Deoxyribose Nucleic Acid) silver staining method for polyacrylamide gel |
| CN103622701B (en) * | 2012-08-23 | 2018-08-10 | 聂棱 | Silanization nano-magnetic powder shows fingerprint method |
| CN103622701A (en) * | 2012-08-23 | 2014-03-12 | 武汉哇哇噻纳技术开发有限公司 | Method for showing fingerprints through silanization nanometer magnetic powder |
| CN103070691B (en) * | 2012-11-09 | 2014-11-05 | 哈尔滨师范大学 | Method for manifesting latent fingerprints on basis of fluorescent gold nanoclusters |
| CN103070691A (en) * | 2012-11-09 | 2013-05-01 | 哈尔滨师范大学 | Method for manifesting latent fingerprints on basis of fluorescent gold nanoclusters |
| CN103356199A (en) * | 2013-07-19 | 2013-10-23 | 北京科技大学 | Method for manifesting metal surface hidden fingerprints through chemical sedimentation |
| CN103536296A (en) * | 2013-10-18 | 2014-01-29 | 浙江大学 | Method of detecting human sweat secretion |
| CN103536296B (en) * | 2013-10-18 | 2015-06-17 | 浙江大学 | Method of detecting human sweat secretion |
| CN103536297B (en) * | 2013-10-18 | 2015-06-17 | 浙江大学 | Immune colloidal gold based latent fingerprint imaging method |
| CN103536297A (en) * | 2013-10-18 | 2014-01-29 | 浙江大学 | Immune colloidal gold based latent fingerprint imaging method |
| CN106073795A (en) * | 2016-06-02 | 2016-11-09 | 西南政法大学 | Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method and Sweat latent fingerprint process for show |
| CN106073795B (en) * | 2016-06-02 | 2019-04-05 | 西南政法大学 | Gold nanoclusters Sweat latent fingerprint visualization reagent preparation method and Sweat latent fingerprint process for show |
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| CN106872437A (en) * | 2017-04-14 | 2017-06-20 | 南京工业大学 | MOFs precursor-based fingerprint rapid-visualization reagent and application thereof |
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