CN106047972A - Novel method for recycling high-concentration organic wastewater - Google Patents
Novel method for recycling high-concentration organic wastewater Download PDFInfo
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- CN106047972A CN106047972A CN201610588547.7A CN201610588547A CN106047972A CN 106047972 A CN106047972 A CN 106047972A CN 201610588547 A CN201610588547 A CN 201610588547A CN 106047972 A CN106047972 A CN 106047972A
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- enzymolysis
- wastewater
- organic wastewater
- supernatant
- precipitation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
Abstract
The invention utilizes bio-enzymolysis and modern bio-separation technologies and provides a novel method for recycling high-concentration organic wastewater. The novel method has the advantages that through a preparation process coordinating gradient separation with ultrasonic-assisted secondary enzymolysis, functional active substances like protein and polysaccharide in the high-concentration organic wastewater generated during deep processing of wheat are fully separated out and biologically modified to obtain products like protein peptide and pentosan higher in functional activity. Under the process condition, up-to-standard discharging of the organic wastewater is realized, sewage treatment cost can be saved by more than 200 RMB for each ton of wastewater, and the environment-protection benefit is good.
Description
Technical field
The present invention relates to a kind of high concentrated organic wastewater resource recycling new method, belong to grain processing byproduct comprehensive
Utilize technical field.
Background technology
Semen Tritici aestivi is as China staple food grain crop, in addition to being converted into flour and Flour product in a large number, along with China's grain yield
Cumulative year after year, increasing Semen Tritici aestivi is used for deep processing and converts, and most typical application is to produce wheaten starch and gluten meal is (little
Aleuronat powder), the further biofermentation of wheaten starch produces ethanol.The most both at home and abroad in wheaten starch, gluten meal processing the most generally
Using two kinds of techniques, one is traditional dough batter process (i.e. Martin's method), and another is starch adhesive partition method (three-phase decanter method).No
Which kind of method is opinion take, and because being wet separation, must produce substantial amounts of processing waste water, the most domestic commonly used dough
Batter process, often produces 1 ton of starch 5-10 to be produced ton waste water.These waste water still contain starch, protein, pentosan
The material such as (or claim araboxylan), soluble dietary fiber, phytic acid, COD, BOD value reaches up to tens the most up to a million, as
Fruit does not make any process and is difficult to reach discharge standard.Some Domestic enterprise muscle waste water of taking to wash one's face this mixes continuation with starch slurry
The technique of fermenting and producing ethanol solves the transition problem of wherein most starch, but the protein of the overwhelming majority and non-starch are many
Glucide is still in waste water.At present Some Domestic enterprise by techniques such as precipitating, concentrate, be dried directly by this waste water
Insoluble matter albumen, the material sediment such as dietary fiber is dried and produces low value-added feedstuff in addition trans-utilization.But, at supernatant
Layer waste liquid still suffers from the soluble protein that enriches and polysaccharide material is difficult to recycle, only as discharge of wastewater, follow-up dirt
Water harnessing cost is the highest.Along with domestic for the raising of environmental requirement, wheaten starch, ethanol deep-processing factory are because of waste water control
Cost and living environment more and more difficult.According to soluble polysaccharide contained in this effluent part of domestic and international research report-
Araboxylan is that one has the different physiological roles such as immunomodulating, blood fat reducing and loosening bowel to relieve constipation, added value pole in fact
High active polysaccharide.The state such as the U.S., Japan is in the i.e. similar active polysaccharide of once extraction from Testa oryzae of the nineties in last century
(Biobran, or claim MGN3), market value high (about 30,000 yuan/kg).Domestic not yet have similar products to develop.Another from waste water
From the point of view of the utilization of protein resource, the country such as American-European lacks economy, mucedin allergic human population generally etc. because of Semen Tritici aestivi relatively Semen Maydis,
The deep processing conversion aspect industrialization level of wheat protein is the highest, and its research focus focuses mostly on how to reduce Semen Tritici aestivi and paddy
The anaphylaxis aspect of protein powder, rarely seen have the research report extracting this albuminoid from garbage, the market demand of its feedstuff industry
Also mainly met by from China's import.Separately there are some researches show the wheat protein conversion through particular organisms enzyme, can generate life
Object has good promotion intestinal injury reparation and the bioactive peptide-glutamine peptide of trophic function.This DMV company of product Holland exists
The most on sale, staple market is towards body-building group, and market price higher (200-300 unit/kg), because price is higher the most current
Still lack market.And in internal feed industry, especially piglet feed produces and has bigger demand to this type of peptide, but at present
Supply product by Feedstock treating, enzyme chooses and technology controlling and process is limited, product quality and effect are difficult to ensure, scarce on market
The peptide product of weary high glutamine content.
Separately from domestic disclosed patent, report and patent application that wheat starch wastewater processes are the most rare.Disclosed
" make gluten remaining starch water reuse method (201110027334) " in be only that the starch in waste water is recycled
Make a kind of Speciality Foods-wine skin；" a kind of wheaten starch and alcohol waste water BYSB-plus water distributor special
(201120353476) ", " a kind of wheaten starch and alcohol waste water BYSB-plus special assembled three phase separator
(201120353893) " it is the utility model patent of two example wastewater treatment equipments.The most apparently, the warp of this part by-product resource
Ji, society and ecological benefits not yet find full expression.
Summary of the invention
Pin the problems referred to above of the present invention, utilize biological enzymolysis and modern biotechnology isolation technics, it is provided that a kind of high concentration organic waste
Recycling of water resource recycling new method.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The present invention provides a kind of high concentrated organic wastewater resource recycling new method, it is characterised in that specifically include following step
Rapid:
(1) Wastewater Pretreatment: regulation wastewater pH is to 6.5～7.0；
(2) sedimentation: utilize natural subsidence to be separated by macromole insoluble substance in waste water；
(3) centrifugal: the supernatant after sedimentation is centrifuged by the centrifuge utilizing rotating speed to be not less than 3000r/min, removes phytic acid；
(4) ultrasound-assisted enzymolysis: add wastewater quality the ratio 1～flavor protease of 2%, 1～2% in the supernatant after centrifugal
Papain, enzymolysis 20～30min under the conditions of ultrasonic assistant, hydrolysis temperature 50～60 DEG C, ultrasonic power 300W, the phase
Between use blender at the uniform velocity stir；
(5) concentrate: enzymolysis solution is concentrated into the 1/2～2/3 of original volume；
(6) isoelectric precipitation: thoroughly do away with destination protein matter, the isoelectric point, IP of polypeptide utilizes isoelectric point method to carry out precipitate and separate；
(7) separate: being separated by the solid-liquid after isoelectric precipitation, solid is protein peptide；
(8) ultrasound-assisted enzymolysis: mass ratio 1～the non-starch polysaccharide enzyme of 2% in the supernatant after isoelectric precipitation separates,
Enzymolysis 20～30min under the conditions of ultrasonic assistant, hydrolysis temperature 40～50 DEG C, ultrasonic power 300W, period employing blender
At the uniform velocity stir；
(9) concentrate: the enzymolysis solution after enzymolysis is concentrated into the 1/3 of original volume;
(10) alcohol analysis: 95% ethanol being gradually added equal-volume ratio in solution precipitates；
(11) pulverizing is dried: the precipitation of acquisition dried and i.e. obtain Semen Tritici aestivi polyose after pulverizing.
The present invention provides a kind of high concentrated organic wastewater resource recycling new method, it is advantageous that: divided by echelon
Work in coordination with preparation technology from ultrasonic assistant secondary enzymolysis, by Semen Tritici aestivi deep processing produce high concentrated organic wastewater in albumen and
The functional activity materials such as polysaccharide are sufficiently separated out, and carry out bio-modification and obtain the more preferable protein peptide of functional activity, pentosan
Deng product.Result of the test shows that protein extracting ratio reaches more than 95% at the process conditions, and polysaccharide extract rate reaches more than 92%,
In albumen after separation, polypeptide accounts for more than the 80% of total product content, and glutamine content >=23%(accounts for Tot Prot)；Analyze
Composition from polysaccharide is mainly pentosan and part xylan.Additionally, result of the test shows to change process conditions or parameter can show
Write and reduce product extraction ratio and organic wastewater resources regeneration comprehensive utilization ratio (P < 0.01)；At the process conditions, organic wastewater is real
Having showed qualified discharge, waste water per ton can save waste water control cost more than 200 yuan, has preferable environmental benefit, and this is special at present
Profit achieves industrialization Transformation Application, annual benefit more than 30,000,000 yuan in company.
Detailed description of the invention
Embodiment 1:
A kind of high concentrated organic wastewater resource recycling new method, it is characterised in that specifically include following steps:
(1) Wastewater Pretreatment: regulation wastewater pH is to 6.5～7.0；
(2) sedimentation: utilize natural subsidence to be separated by macromole insoluble substance in waste water；
(3) centrifugal: the supernatant after sedimentation is centrifuged by the centrifuge utilizing rotating speed to be not less than 3000r/min, removes phytic acid；
(4) ultrasound-assisted enzymolysis: add the wastewater quality flavor protease than 2%, the Fructus Chaenomelis of 2% in the supernatant after centrifugal
Protease, enzymolysis 20min under the conditions of ultrasonic assistant, hydrolysis temperature 50～60 DEG C, ultrasonic power 300W, period uses stirs
Machine at the uniform velocity stirs；
(5) concentrate: enzymolysis solution is concentrated into the 1/2～2/3 of original volume；
(6) isoelectric precipitation: thoroughly do away with destination protein matter, the isoelectric point, IP of polypeptide utilizes isoelectric point method to carry out precipitate and separate；
(7) separate: being separated by the solid-liquid after isoelectric precipitation, solid is protein peptide；
(8) ultrasound-assisted enzymolysis: the non-starch polysaccharide enzyme of mass ratio 2% in the supernatant after isoelectric precipitation separates, super
Enzymolysis 20min under sound wave subsidiary conditions, hydrolysis temperature 40～50 DEG C, ultrasonic power 300W, period employing blender at the uniform velocity stirs；
(9) concentrate: the enzymolysis solution after enzymolysis is concentrated into the 1/3 of original volume;
(10) alcohol analysis: 95% ethanol being gradually added equal-volume ratio in solution precipitates；
(11) pulverizing is dried: the precipitation of acquisition dried and i.e. obtain Semen Tritici aestivi polyose after pulverizing.
Embodiment 2:
A kind of high concentrated organic wastewater resource recycling new method, it is characterised in that specifically include following steps:
(1) Wastewater Pretreatment: regulation wastewater pH is to 6.5～7.0；
(2) sedimentation: utilize natural subsidence to be separated by macromole insoluble substance in waste water；
(3) centrifugal: the supernatant after sedimentation is centrifuged by the centrifuge utilizing rotating speed to be not less than 3000r/min, removes phytic acid；
(4) ultrasound-assisted enzymolysis: add the wastewater quality flavor protease than 1%, the Fructus Chaenomelis of 1% in the supernatant after centrifugal
Protease, enzymolysis 30min under the conditions of ultrasonic assistant, hydrolysis temperature 50～60 DEG C, ultrasonic power 300W, period uses stirs
Machine at the uniform velocity stirs；
(5) concentrate: enzymolysis solution is concentrated into the 1/2～2/3 of original volume；
(6) isoelectric precipitation: thoroughly do away with destination protein matter, the isoelectric point, IP of polypeptide utilizes isoelectric point method to carry out precipitate and separate；
(7) separate: being separated by the solid-liquid after isoelectric precipitation, solid is protein peptide；
(8) ultrasound-assisted enzymolysis: the non-starch polysaccharide enzyme of mass ratio 1% in the supernatant after isoelectric precipitation separates, super
Enzymolysis 30min under sound wave subsidiary conditions, hydrolysis temperature 40～50 DEG C, ultrasonic power 300W, period employing blender at the uniform velocity stirs；
(9) concentrate: the enzymolysis solution after enzymolysis is concentrated into the 1/3 of original volume;
(10) alcohol analysis: 95% ethanol being gradually added equal-volume ratio in solution precipitates；
(11) pulverizing is dried: the precipitation of acquisition dried and i.e. obtain Semen Tritici aestivi polyose after pulverizing.
Embodiment 3:
A kind of high concentrated organic wastewater resource recycling new method, it is characterised in that specifically include following steps:
(1) Wastewater Pretreatment: regulation wastewater pH is to 6.5～7.0；
(2) sedimentation: utilize natural subsidence to be separated by macromole insoluble substance in waste water；
(3) centrifugal: the supernatant after sedimentation is centrifuged by the centrifuge utilizing rotating speed to be not less than 3000r/min, removes phytic acid；
(4) ultrasound-assisted enzymolysis: the supernatant after centrifugal adds the wastewater quality flavor protease than 1.5%, 1.5%
Papain, enzymolysis 25min under the conditions of ultrasonic assistant, hydrolysis temperature 50～60 DEG C, ultrasonic power 300W, period employing
Blender at the uniform velocity stirs；
(5) concentrate: enzymolysis solution is concentrated into the 1/2～2/3 of original volume；
(6) isoelectric precipitation: thoroughly do away with destination protein matter, the isoelectric point, IP of polypeptide utilizes isoelectric point method to carry out precipitate and separate；
(7) separate: being separated by the solid-liquid after isoelectric precipitation, solid is protein peptide；
(8) ultrasound-assisted enzymolysis: the non-starch polysaccharide enzyme of mass ratio 1.5% in the supernatant after isoelectric precipitation separates,
Enzymolysis 25min under the conditions of ultrasonic assistant, hydrolysis temperature 40～50 DEG C, ultrasonic power 300W, period employing blender at the uniform velocity stirs
Mix；
(9) concentrate: the enzymolysis solution after enzymolysis is concentrated into the 1/3 of original volume;
(10) alcohol analysis: 95% ethanol being gradually added equal-volume ratio in solution precipitates；
(11) pulverizing is dried: the precipitation of acquisition dried and i.e. obtain Semen Tritici aestivi polyose after pulverizing.
The present invention provides a kind of high concentrated organic wastewater resource recycling new method, by animal experiment research polysaccharide
Functional, primary study polysaccharide is preventing constipation, is reducing effect of the aspect such as cholesterol, the liver protecting, and result shows: (1)
Mice with constipation model test shows that the polysaccharide prepared has function of relaxing bowel, after per os gives the polysaccharide of mice various dose,
Each dosage group all can substantially increase the Small intestine advance activity of mice, shortens the defecation time first of mice with constipation, increases constipation little
The defecation grain number of Mus and defecation weight, and in process of the test, each dosage group is showed no diarrhoea phenomenon, illustrates that mice gives agent
Rationally, the form of feces is pitchy graininess to amount, and feces is the softest compared with control group mice feces, surface wettability, feces
Moistening degree is moderate, and sense organ is normal；(2) by mouse feeding high lipid food, hyperlipemia in mice model is set up, to try
Test research.Result of the test shows, blank group may determine that with model control group blood lipid level difference statistically and makes
Mould success.Test group can significantly reduce TC, TG, LDL-c, level (P < 0.01) in pole compared with model control group；(3) by height
The mensuration of lipidemia mouse blood sugar value, is compared with model control group by blank group, illustrates that mouse blood sugar model is set up, test
Result shows, Polysaccharides on Mice has preferable function of blood sugar reduction, with model control group significant difference (P < 0.01).
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to aforementioned reality
Execute example to be described in detail by invention, but for the person of ordinary skill of the art, still can be to aforementioned reality
Execute the technical scheme described in example to modify, or wherein portion of techniques feature is carried out equivalent；And these are revised
Or replace, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (1)
1. high concentrated organic wastewater resource recycling new method, it is characterised in that specifically include following steps:
(1) Wastewater Pretreatment: regulation wastewater pH is to 6.5～7.0；
(2) sedimentation: utilize natural subsidence to be separated by macromole insoluble substance in waste water；
(3) centrifugal: the supernatant after sedimentation is centrifuged by the centrifuge utilizing rotating speed to be not less than 3000r/min, removes phytic acid；
(4) ultrasound-assisted enzymolysis: add wastewater quality the ratio 1～flavor protease of 2%, 1～2% in the supernatant after centrifugal
Papain, enzymolysis 20～30min under the conditions of ultrasonic assistant, hydrolysis temperature 50～60 DEG C, ultrasonic power 300W, the phase
Between use blender at the uniform velocity stir；
(5) concentrate: enzymolysis solution is concentrated into the 1/2～2/3 of original volume；
(6) isoelectric precipitation: thoroughly do away with destination protein matter, the isoelectric point, IP of polypeptide utilizes isoelectric point method to carry out precipitate and separate；
(7) separate: being separated by the solid-liquid after isoelectric precipitation, solid is protein peptide；
(8) ultrasound-assisted enzymolysis: mass ratio 1～the non-starch polysaccharide enzyme of 2% in the supernatant after isoelectric precipitation separates,
Enzymolysis 20～30min under the conditions of ultrasonic assistant, hydrolysis temperature 40～50 DEG C, ultrasonic power 300W, period employing blender
At the uniform velocity stir；
(9) concentrate: the enzymolysis solution after enzymolysis is concentrated into the 1/3 of original volume;
(10) alcohol analysis: 95% ethanol being gradually added equal-volume ratio in solution precipitates；
(11) pulverizing is dried: the precipitation of acquisition dried and i.e. obtain Semen Tritici aestivi polyose after pulverizing.
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| CN201610588547.7A CN106047972A (en) | 2016-07-25 | 2016-07-25 | Novel method for recycling high-concentration organic wastewater |
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| CN201610588547.7A CN106047972A (en) | 2016-07-25 | 2016-07-25 | Novel method for recycling high-concentration organic wastewater |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613220A (en) * | 2013-11-21 | 2014-03-05 | 中国农业科学院农产品加工研究所 | Method for extracting multiple functional components from wheat starch processing wastewater |
| CN104894189A (en) * | 2015-07-06 | 2015-09-09 | 青岛嘉瑞生物技术有限公司 | Method for extracting xylooligosaccharide from wheat bran |
| CN104921149A (en) * | 2015-07-08 | 2015-09-23 | 青岛嘉瑞生物技术有限公司 | Technology for extracting bran dietary fibers by combining ultrasonic-assisted enzymolysis and microbial fermentation |
-
2016
- 2016-07-25 CN CN201610588547.7A patent/CN106047972A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613220A (en) * | 2013-11-21 | 2014-03-05 | 中国农业科学院农产品加工研究所 | Method for extracting multiple functional components from wheat starch processing wastewater |
| CN104894189A (en) * | 2015-07-06 | 2015-09-09 | 青岛嘉瑞生物技术有限公司 | Method for extracting xylooligosaccharide from wheat bran |
| CN104921149A (en) * | 2015-07-08 | 2015-09-23 | 青岛嘉瑞生物技术有限公司 | Technology for extracting bran dietary fibers by combining ultrasonic-assisted enzymolysis and microbial fermentation |
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