CN102058018A - Animal feed additive and application thereof - Google Patents

Abstract

The invention relates to an animal feed additive and application thereof. Residue obtained after microbial oil is extracted is taken as a raw material, and is hydrolyzed to prepare the animal feed additive. The additive is rich in proteins, also contains a certain amount of oil and crude fibers, and is a nutritious protein feed. The animal feed additive is simple in preparation process, wide in production raw material sources, contributes to large-scale production, solves the problem that the residue obtained after the microbial oil is extracted has high crude fiber content and is difficultly digested and absorbed by animals, reduces the production cost of the microbial oil, reduces environmental pollution caused by waste materials and promotes comprehensive utilization of resources.

Description

A kind of animal feed additive and application thereof

Technical field

The present invention relates to animal-breeding feed, specifically a kind of preparation method of animal feed additive and application thereof.

Background technology

Along with the raising of economic development and living standards of the people, the mankind have stimulated the development of animal husbandry and aquaculture greatly to the demand of high nutritive value animal protein.The simple feed market that relies on traditional approach to provide high-quality proteins such as soybean cake, fish meal can not satisfy rapid growth, thus people often use mycoprotein as an alternative product solve the deficiency of protein feed supply.

Mycoprotein claims single cell protein again, typically refer to by cultivating the bacterium protein that unicellular microorganism obtains, wherein protein content is up to 40%～80%, be rich in simultaneously polysaccharide, vitamin, mineral matter and unknown somatomedin etc. (Xiong Zhihui. the progress of single cell protein in feed industry, culture and feed, 2006, (7), 11-15).Mycoprotein has good palatability, can strengthen effects such as animal immunizing power.

In China, about the research of exploitation and popularization microbial fermentation production mycoprotein, more concentrating on used on non-edible resource and the waste resource.But, still exist production cost to cross problems such as height because raw material sources are limited or conversion ratio is low.Simultaneously, nucleic acid content is too high because mycoprotein exists in application process, the amino acid supply is uneven, and contain composition such as stodgy mannosan, often animal health or feed digestion are had side effects, thereby limited its use amount in the animal daily ration greatly.

Some oleaginous microorganism, as bacterium, saccharomycete, mould and algae etc., under given conditions, the grease that can surpass its biological total amount 20% (w/w) at intracellular accumulation, especially in eukaryotic microorganisms, some produce oil kind can surpass its grease more than biological total amount 70% (w/w) at intracellular accumulation, these greases mainly exist with the form of fatty acid triglycercide, it forms similar (Ratledge C to general animal and plant fat, Wynn JP.The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms.Advances in Applied Microbiology, 2002,51,1-52).Well-known is that the microbial grease production of purpose has realized industrialization in states such as Japan and the United States and Sweden to obtain polyunsaturated fatty acid (PUFAs).Microbe oil fermentation also becomes biodiesel industry and bioeconomic important research direction gradually in recent years.

Because microbial grease is intracellular product, bacterium slag that association is a large amount of when obtaining grease.No matter miscellaneous oleaginous microorganism is eucaryon or prokaryotic micro-organisms, and the residue behind the extraction grease all is the potential source of feeding mycoprotein.Especially the yeast of high yield grease, mould and little algae etc., all higher because of its fat content and biological safety, receive much concern in recent years.To produce the research of microbial greases also more and more for various non-grain crops (as jerusalem artichoke, cassava, sweet potato etc.), agriculture and forestry discarded object (as maize straw), industrial wastes and discarded object etc. to utilize these oleaginous microorganisms to ferment.

Industrial grease extracting method commonly used comprises milling process, lixiviation process and supercritical fluid extraction (ZL 03139630.5) etc.Simultaneously, in order to improve the grease yield, also often adopt different pre-treating methods at different production bacterial classifications, as earlier will steaming stir-fry after the thalline drying again, or carry out enzyme hydrolysis (as autolysis method or enzymatic isolation method), physics fragmentation (as grinding, ultrasonic disruption, microwave method, freeze-thaw method or desivac) or chemical Treatment methods such as (as with acid heat method, fat-soluble organic solvent or surfactant processing) in advance and make the thalline broken wall.These wall-breaking methods, some only is applicable to that the grease of laboratory small amount of sample extracts, and some is then handled because of the violent acid heat of process, or adds the chemical reagent that some is difficult for removing, and the nutritional labeling in the thalline is subjected to very big destruction, and the bacterium slag using value of gained is lower.Therefore in extensive grease leaching process, select suitable grease extracting method and bacterium slag post-processing approach very necessary, this not only helps accelerating the process of industrialization of industries such as microbial grease and biodiesel, more can promote comprehensive utilization of resources, solve the problems such as short supply of mycoprotein raw materials for production.

Except containing a small amount of grease, also contain more protein and cell wall constituent in the microbe oil fermentation bacterium slag.Generally, no solvent residue in squeezing or the bacterium slag that obtains of supercritical fluid extraction, can be directly as mycoprotein in feed applications.It should be noted that the cell wall constituent of microbe oil fermentation bacterium slag, often in the grease leaching process, do not obtain effective hydrolysis, be difficult for being digested and assimilated, the use amount and the feeding effect of appreciable impact bacterium slag by animal.Common saccharomycetic cell membrane based on glucan and (or) mannosan, also have some protein and lipid in addition; The cell membrane of mould mainly is made up of chitin; The alga cells wall mainly is made up of cellulose, also contains different polysaccharide, lipid and protein.On the one hand, polysaccharide such as cell wall polysaccharides such as mannosan, chitin, cellulose except digestibility is low, also usually produce more negative physiological effect to animal; On the other hand, yeast cell wall polysaccharide also can be used as feed addictive, has immunologic function as glucan, and manna oligosacchride also is good " pathogen adsorbent ", often is used in aquaculture feed.In sum, adopt microbe oil fermentation bacterium slag to make feeding mycoprotein,, can play the effect that improves animal immunizing power, widen its scope of application in the animal daily ration, significantly improve feeding effect if in advance it is carried out appropriate hydrolysis.

In the microbial grease leaching process, need composition such as pair cell wall to carry out physics, chemistry and/or biochemistry and handle, make that grease fully discharges in the born of the same parents, to improve the grease yield; The bacterium slag reclaim and the precipitation process in, also can partly remove or passivation some anti-nutrition wherein by adjusting process.But these processing methods do not have very strong specific aim and practicality to the oleaginous microorganism bacterium slag that contains more cell wall polysaccharides.

The invention provides a kind of animal feed additive and preparation method thereof, the residue after promptly extracting with microbial grease is a raw material, by making after the hydrolysis process.This animal feed additive can be used as mycoprotein or feed addictive is used in feed, presses solid content meter, and additive amount can be 0.1%～20% of mixed fodder total amount.The processing method mild condition that the present invention adopts, with strong points.Not only can reduce the production cost of microbial grease, solve waste bacterial slag pollution on the environment problem, also can reduce feed cost simultaneously, reduce the use amount that comprises high price nutritional labelings such as dregs of beans, fish meal and grease in the feed.

Summary of the invention

The present invention aims to provide a kind of animal feed additive and preparation method thereof, residue after promptly extracting with microbial grease is a raw material, after hydrolysis process, directly make total solid concentration and be the liquid additive finished product of 10%～60% (w/w) or make the solid additives finished product 20 ℃～100 ℃ dryings.This additive finished product is used in feed as mycoprotein or feed addictive, presses solid content meter, and additive amount can be 0.1%～20% of mixed fodder total amount.

The raw material sources that the present invention uses surpass the eucaryon or the prokaryotic micro-organisms of its biological total amount 20% (w/w) at the intracellular accumulation grease after fermented and cultured.They include but not limited to, the produce oil bacterium is as Arthrobacter genus, Rhodococcus genus or Mycobacterium genus etc.; The oleaginous yeast bacterium, as Candida genus, Cryptococcus genus, Hansenula genus, Lipomyces genus, Rhodosporidium genus, Rhodotorula genus, Endomyces genus, Schwanniomye genus, Trichosporon genus, Sporobolomyces genus, Trigonopsis genus or Yarrowia genus etc.; The produce oil mould is as Mortierella genus, Cunninghamella genus, Mucor genus or Aspergillus genus etc.; The little algae of produce oil is as Crypthecodinium genus, Botryococcus genus, Chlorella genus, Nannochloropsis genus, Haliphthoros genus or Schizochytrium genus etc.These bacterial strains can be directly from comprising that Chinese common micro-organisms culture presevation administrative center (CGMCC), American type culture collection (ATCC) and Chinese industrial microorganism fungus kind preservation administrative center culture presevation mechanisms such as (CICC) buy or from the occurring in nature separation, also can using the artificial or natural mutation bacterial strain different with original bacterial strain proterties.

Preferred biological safety height in mentioned microorganism, non-causing a disease and the non-bacterial strain that produces poison, as being widely used in producing Mortierella isabellina Mortierella isabellina or the Mortierella alpina Mortierella alpina of GLA and ARA, the Kou Shi that produces DHA conceals dinoflagellate Crypthecodinium cohnii or produce oil chlorella Chlorella protothecoides etc., and produce oil rhodotorula Rhodosporidium toruloides or this Da Shi saccharomyces oleaginosus Lipomyces starkeyi etc., perhaps use the high productive mutant of these bacterial strains, and before feeding, carry out corresponding toxicity test.

Grease extracting method used in the present invention, comprise that milling process, supercritical fluid extraction, organic solvent extractionprocess, acid heat method and other can effectively separate the method that obtains grease from the oleaginous microorganism thalline, but preferred the employing comprises milling process, supercritical fluid extraction and low toxicity organic solvent extractionprocess.

Microbe oil fermentation bacterium pulp water provided by the present invention is separated processing method and is comprised enzyme hydrolysis, acid hydrolysis or the combination of their necessity, and concrete hydrolysising condition is:

(1) enzyme hydrolysis: the residue after the microbial grease extraction is mixed with water, make total solid concentration reach 10%～90% (w/w), add hydrolase to total enzyme concentration and reach 0.005%～3% (w/w), 20 ℃～90 ℃ following insulated and stirred 10 minutes～40 hours.

(2) acid hydrolysis: the residue after the microbial grease extraction is mixed with water, make total solid concentration reach 10%～90% (w/w), with acid for adjusting pH 1.0～5.0,, be neutralized to pH 5.5～7.5 with alkali again 20 ℃～90 ℃ following insulated and stirred 10 minutes～24 hours.

It is a kind of in dextranase, mannase, glucomannan enzyme, cellulase, glusulase or the chitinase or their combination that mentioned microorganism oil fermentation bacterium pulp water is separated hydrolase used in the processing method, and used acid is inorganic acid or organic acid; Used alkali is inorganic base or organic base.

Microbe oil fermentation bacterium slag is as stated above after the hydrolysis process, directly makes total solid concentration and is the liquid additive finished product of 10%～60% (w/w) or make the solid additives finished product 20 ℃～100 ℃ dryings.This additive finished product can be used as mycoprotein or feed addictive is used in feed, presses solid content meter, and additive amount can be 0.1%～20% of mixed fodder total amount.

The invention has the beneficial effects as follows: 1) compare with traditional animal feeding-stuff containing somatic protein, microbe oil fermentation bacterium slag of the present invention through enzyme or acid hydrolysis, effectively reduces the crude fiber content in the bacterium slag, more helps animal and absorbs; 2) in the microbe oil fermentation bacterium slag of the present invention except the carbohydrate that is rich in protein, easily digests and assimilates, also contain a certain amount of grease, can reduce the grease addition in the feed, take into account the balance of energy and protein in the feed formula, reduced feed cost; 3) also contain a certain amount of polyunsaturated fatty acid in the certain micro-organisms oil fermentation bacterium slag of the present invention, materials such as mineral matter, pigment help to improve the immunity of animal; 4) microbe oil fermentation bacterium slag of the present invention can effectively reduce the production cost of microbial grease, reduces the environmental pollution that waste bacterial slag causes, and promotes comprehensive utilization of resources.In a word, preparation technology of the present invention is simple, and raw material sources is wide, and equipment investment is few, promotes easily, helps realizing large-scale industrial production.

The specific embodiment

Following examples have been chosen some typical oleaginous microorganism bacterial strains, illustrated that the residue that extracts behind the grease makes method and the effect thereof of animal feed additive, this will help to understand this patent, but not other oleaginous microorganism material enforcement this patent invention of restriction application in any form.

Embodiment 1

(bacterium source is in Chinese common micro-organisms culture presevation administrative center to adopt shadow yeast Sporobolomyces reseus AS 2.618, CGMCC) be bacterial classification, adopt document (Xiong Wangxian, Tao Yang, Liu Guangye. " fermentation condition of shadow yeast fermentation starch from sweet potato enzyme hydrolyzate produce oil fat " used and the environmental organism journal, 2008,14 (4): 558-561) described optimal medium and the most adaptable method are cultivated thalline, being cultured in the zymotic fluid concentration of reduced sugar is reduced to 1% and finishes when following, collect thalline, after the drying oil content is 40% dry mycelium 380g.Keeping temperature is 35 ℃, and pressure is 50MPa, and dry mycelium was squeezed in oil press 5～6 hours, again filter cake is carried out multiple press for extracting juice, squeezes crude oil 110g altogether, reclaims solid bacterium slag 260g.To be adjusted to total solid concentration be 10% with adding entry in the bacterium slag after the squeezing, adds the mannase and the dextranase that mix by 1: 1 part by weight, to the total enzyme weight concentration of mixed liquor be 1%, 37 ℃ of insulated and stirred 40 hours.80 ℃ down dry, pulverize solid-state animal feed additive finished product 275g.This finished product is a brown powder, and band fat fragrance contains crude fat 12%, crude protein 32%, crude fibre 5%, ash content 5%, moisture 9%.

Embodiment 2

Adopting red winter spore Rhodosporidium toruloides AS 2.1389 (bacterial classification derives from CGMCC) of circle is bacterial classification, according to document (Hua Yanyan, Zhao Xin, Zhao Jin, Zhang Sufang, Zhao Zongbao. the research of the red winter spore culture propagation jerusalem artichoke stem tuber produce oil fat of circle, Chinese biological engineering magazine, 2007,27 (10): 59-63.) described method, collect the thalline that the white skin jerusalem artichoke clear liquid of fermentation is obtained, can obtain oil content after the drying and be 62% dry mycelium 585g.In grinder dry mycelium is clayed into power, press 100g bacterium powder and added the 0.2L n-hexane extraction 5 hours, Separation of Solid and Liquid adds the n-hexane re-extract twice again.Merge and to extract crude oil 330g altogether after the n-hexane layer is removed solvent, by document (how to conquer east the Chen Tao chief editor. microbial grease is learned, Beijing: Chemical Industry Press, 2005.12) described method carries out carrying out precipitation treatment, recovery solid bacterium slag 255g.Aforementioned bacterium slag being mixed with suitable quantity of water to be adjusted to total solid concentration be 90%, is 1.5 with the sulphur acid for adjusting pH value, and 90 ℃ of insulated and stirred 20 minutes, cool to room temperature was neutralized to pH 5.5～6.0 with sodium hydroxide solution.Continue to add water, being adjusted to total solid concentration is 30%, gets liquid animal feeds additive finished product 850mL.This finished product is the brown suspension, and slightly fragrance contains crude fat 3.3%, crude protein 10%, crude fibre 2%, ash content 1.3%.

Embodiment 3

Adopting rhodotorula glutinis Rhodotorula glutinis AS 2.703 (bacterium source is in CGMCC) is bacterial classification, with reference to " method of microbiological treatment trade waste and the method for prepare microbial grease by fermentation and special strain therefore thereof (number of patent application 200710179996) " disclosed thalline cultural method, the somatic cells that collection utilizes starch wastewater cultivate to obtain obtains oil content and is 35% dry mycelium 300g after the drying.With reference to " extracting method of the supercritical carbon dioxide extracting of microbial grease (patent No. ZL03139630.5) " disclosed grease extracting method, at volume is in the extraction kettle of 1L, extracting pressure is 15MPa, extraction temperature is 45 ℃, parsing pressure is 5MPa, resolution temperature is 35 ℃, reaches that total extraction time is 8～10 hours after the extracting pressure.Coextraction crude oil 95g from extraction-container, residue bacterium slag adds entry, and to be adjusted to total solid concentration be 40%, adding glucomannan enzyme total enzyme concentration to the mixed liquor is 2.5%, 50 ℃ of insulated and stirred 1 hour, get bacterium slag hydrolyzate, 70 ℃ down dry, pulverize solid-state animal feed additive finished product 210g.This finished product is a pink powder, and band fat fragrance contains crude fat 3%, crude protein 32%, crude fibre 5%, ash content 6%, moisture 6%.

Embodiment 4

Adopting Mortierella isabellina Mortierella isabellina AS 3.3410 (bacterium source is in CGMCC) is bacterial classification, with glucose is fermentation raw material, cultivate and the grease extracting method with reference to ZL 00116051.6 disclosed thalline, all move into spore inoculating cultivation in the seeding tank that the 120L material is housed in the fermentation tank of 1200L after 45 hours, cultivated 96 hours, and be reduced to 1% at reduced sugar and put jar when following.Adopt plate-press dryer 80 ℃ dry down, obtain oil content and be 55% dried mycelium 50kg.In oil press, after under 105 ℃ dry mycelium steam being fried 20min, squeezed 3～4 hours, obtain crude oil 25kg, residue bacterium slag 24.5kg with indirect steam.Residue bacterium slag adds entry, and to be adjusted to total solid concentration be 30%, and adding chitinase to total enzyme concentration is 0.3%, 60 ℃ of insulated and stirred 10 hours, bacterium slag hydrolyzate, 80 ℃ dry down, pulverize solid-state animal feed additive finished product 26.5kg.This finished product is the yellowish-brown powder, and slightly fragrance contains crude fat 6%, crude protein 22%, crude fibre 11%, coarse ash 7%, moisture 9%.

Embodiment 5

(bacterium source ATCC) is bacterial classification in American type culture collection, with reference to embodiment 1 described thalline cultural method, can obtain oil content and be 37% dried mycelium 34kg to adopt Mortierella alpina Mortieralla Alpina ATCC 32222.The grease extracting method is squeezed crude oil 11kg altogether with embodiment 1.With adding entry to total solid concentration in the bacterium cake after the squeezing is 60%, and interpolation chitinase to enzyme concentration is 0.005%, 20 ℃ of insulated and stirred 10 minutes; Add entry again and be diluted to total solid concentration and reach 10%, regulate 4.0,20 ℃ of insulated and stirred of pH 1 hour, be neutralized to pH 7.0 with sodium hydroxide solution again with acetate, bacterium slag hydrolyzate, 80 ℃ dry down, pulverize solid-state animal feed additive finished product 24kg.This finished product is a brown powder, and band fat fragrance contains crude fat 5%, crude protein 30%, crude fibre 8%, ash content 4%, moisture 9%.

Embodiment 6

Adopting Blang grape algae Botryococcus braunii LB572 (bacterium source is in DSMZ of Texas ,Usa university) is bacterial classification, reference literature (Xu H, Miao XL, Wu QY.High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters.J Biotechnol, 2006,126 (4): 499-507.) described culture medium and fermentation condition, with glucose is raw material, add glycine, being cultured in the zymotic fluid concentration of reduced sugar is reduced to 1% and finishes when following, obtaining dry cell weight is the zymotic fluid 10L of 60g/L (oil content 55%), collect thalline, the grease extracting method is extracted crude oil 290g altogether with embodiment 3, residue algae-residue 305g, algae-residue is mixed with water, to total solid concentration be 40%, adding cellulase to enzyme concentration is 3%, 60 ℃ of insulated and stirred 12 hours.It is 10% that aforementioned hydrolyzate is diluted with water to total solid concentration, gets liquid animal feeds additive finished product 3.0L.This finished product is the bottle green suspension, and slightly fragrance contains crude fat 1.2%, crude protein 3.5%, crude fibre 0.7%, ash content 0.4%.

Embodiment 7

Adopting latent dinoflagellate Crypthecodinium cohnii ATCC 30772 (bacterium source is in ATCC) of Kou Shi is bacterial classification, with glucose is primary raw material, with reference to the disclosed alginic cell cultural method of CN 1986822A, inoculum concentration is 10%, keep 25 ℃ of jar temperature, tank pressure 0.09kPa, stir speed (S.S.) 150rpm fermented 120 hours.Be reduced to 0.5% at reduced sugar and put jar when following.Zymotic fluid is after concentrating, and obtaining dry cell weight is the zymotic fluid 60L of 53g/L (oil content is 42%).Add the chloroform with concentrated broth volume equivalent, 25 ℃ of following stirring and leaching 3 hours, sedimentation separation.Take off a layer chloroform layer, obtain crude oil 1.1kg behind the evaporating solvent.Collect the upper strata, centrifugal treating obtains the method carrying out precipitation treatment of wet algae-residue by embodiment 2, obtains algae-residue 2.8kg after the drying.It is 50% that algae-residue and water are mixed to total solid concentration, with the lemon acid for adjusting pH value is 5.0, add cellulase to its concentration and be 0.03% and glusulase to its concentration be 0.1%, at 50 ℃, insulated and stirred 3 hours, be neutralized to pH 7.0 with ammonia spirit again, 60 ℃ down dry, pulverize solid-state animal feed additive finished product 2.9kg.This finished product is a brown powder, and slightly fragrance contains crude fat 7%, crude protein 25%, crude fibre 6%, ash content 3%, moisture 9%.

Embodiment 8

Adopting red winter spore Rhodosporidium toruloides AS 2.1389 (bacterial classification derives from CGMCC) of circle is bacterial classification, according to document (Hua Yanyan, Zhao Xin, Zhao Jin, Zhang Sufang, Zhao Zongbao. the research of the red winter spore culture propagation jerusalem artichoke stem tuber produce oil fat of circle, Chinese biological engineering magazine, 2007,27 (10): 59-63.) described method, collect the thalline that the white skin jerusalem artichoke clear liquid of fermentation is obtained, can obtain oil content after the drying and be 62% dry mycelium 585g.In grinder dry mycelium is clayed into power, press 100g bacterium powder and added the 0.2L n-hexane extraction 5 hours, Separation of Solid and Liquid adds the n-hexane re-extract twice again.Merge and to extract crude oil 330g altogether after the n-hexane layer is removed solvent, by document (how to conquer east the Chen Tao chief editor. microbial grease is learned, Beijing: Chemical Industry Press, 2005.12) described method carries out carrying out precipitation treatment, recovery solid bacterium slag 255g.Aforementioned bacterium slag being mixed with suitable quantity of water to be adjusted to total solid concentration be 90%, is 2.0 with the vinegar acid for adjusting pH value, and 90 ℃ of insulated and stirred 40 minutes, cool to room temperature was neutralized to pH 6.0 with sodium carbonate liquor.Continue to add water, being adjusted to total solid concentration is 60%, gets liquid animal feeds additive finished product 430mL.This finished product is the brown viscous fluid, and slightly fragrance contains crude fat 6.5%, crude protein 20%, crude fibre 4.1%, ash content 2.7%.

Following examples have been chosen representational animal feed additive finished product in the foregoing description; add in the animal feed; animals such as chicken, pig, milk cow or fish are used to feed; this will help further to understand this patent, but not limit animal feed additive finished product of the present invention in any form and addition manner is used in other fowl, poultry or aquatic animal feed.

The application of application examples 1 in broiler fodder

The solid-state animal feed additive finished product of embodiment 1 preparation, through acute toxicity test, feeding test for 7 days and mutagenicity test, its result are nontoxic product in advance, do not have tangible mutation effect, can partly replace dregs of beans, imported fish meal and vegetable oil in broiler fodder, to use as protein feed.

Experimental group feed formula: corn flour 55.3%, dregs of beans 26%, solid-state animal feed additive finished product 15%, grease 1.2%, lysine 0.14%, methionine 0.12%, vitamin 0.01%, calcium phosphate 1.0%, salt 0.3%, stone flour 0.93%.

Trophic level: crude protein 20.2%, lysine 1.08%, methionine 0.41%, calcium 0.61%, phosphorus 0.78%, metabolizable energy 12.12 million Jiao/kilograms.

The control group feed formula: Dalian China becomes fryer concentrated feed 35%, corn flour 65%.

Test is to like to pull out increasingly 3 ages in week of (Arbor Acres) fryer with chicken, each 200 of experimental group and control groups, and random packet, the raising condition is identical with management condition, and deliver for sale 8 all ages.

The result that feeds is as follows: the average end of experimental group weighs 3.27 kilograms/, feedstuff-meat ratio 1.83: 1; The average end of control group weighs 3.02 kilograms/, feedstuff-meat ratio 1.91: 1.

The application of application examples 2 in the growing and fattening pig feed

The animal feed additive finished product of embodiment 5 preparations, through acute toxicity test, feeding test for 7 days and mutagenicity test, its result are nontoxic product, do not have tangible mutation effect, can partly replace dregs of beans, imported fish meal and vegetable oil as the application of protein feed in growth big porker feed.

Experimental group feed formula: corn flour 60%, wheat bran 14%, soya-bean cake 4%, animal feed additive finished product 20%, oyster shell whiting 0.70%, bone meal 0.82%, lysine 0.23%, salt 0.25%.

Trophic level reference value: crude protein 15.09%, lysine 0.74%, methionine+cystine 0.42%, calcium 0.6%, phosphorus 0.5%, salt 0.25%, digestible energy 13.86 million Jiao/kilograms.

The control group feed formula: China becomes 838 concentration pig fodders 20%, corn flour 75%, wheat bran 5%.

Test with pig is: Du Changda three way cross kind, and average 26.6 kilograms/head of starting weight, each 80 of experimental group and control groups, random packet, similarity condition was raised 100 days.

The result that feeds is as follows: the average end of experimental group weighs 108.5 kilograms/head, feedstuff-meat ratio 3.42: 1, and the average end of control group weighs 103.5 kilograms/head, feedstuff-meat ratio 3.68: 1.

The application of application examples 3 in fish feed

Press the solid-state animal feed additive finished product of embodiment 7 methods preparation, through acute toxicity test, feeding test for 7 days and mutagenicity test, its result is nontoxic product, does not have tangible mutation effect, can be used as additive of protein feed and uses in fish feed.

Control group feed formula: dregs of beans 25%, fish meal 3%, wheat bran 62%, inferior powder 10%.

The experimental group feed formula: on control group feed formula basis, add the solid-state animal feed additive finished product of pressing the preparation of embodiment 7 methods, consumption is 5%.

Experimental group feed nutrition level: crude protein 34.1%, crude fat 5.9%, crude fibre 7.6%, coarse ash 6.2%, soluble-salt 1.5%.

Control group feed nutrition level: crude protein 32.3%, crude fat 5.3%, crude fibre 7.2%, coarse ash 6.0%, soluble-salt 1.4%.

Fish culture in net pen is used in test, and experimental group and control group are gross weight 200kg when putting in a suitable place to breed, average cabrage 95g.During results, experimental group gross weight 901kg, average cabrage 450g, control group gross weight 850kg, average cabrage 437g.This shows that add 5% solid-state animal feed additive finished product as the feed addictive fish of feeding in mixed fodder, effect of increasing production reaches 6%, the fry death rate obviously reduces.

The application of application examples 4 in milk cow forage

Press the liquid animal feeds additive finished product of embodiment 2 methods preparation, through acute toxicity test, feeding test for 7 days and mutagenicity test, its result is nontoxic product, does not have tangible mutation effect.Can be used as feed addictive uses in milk cow forage.

Test with milk cow is: 80 of china holstein cowses, and body condition is normal, and the output of milk and the phase of giving milk are approaching, are divided into two groups at random, and one group is experimental group (40), and another group is control group (40).The difference of experimental group and control group is, liquid animal feeds additive finished product is sprayed at the surface of the mixed fodder of throwing something and feeding every day, and consumption accounts for 0.8 ± 0.1% of the mixed fodder total amount of at every turn throwing something and feeding, and 30 days experimental periods, milks the record output of milk every day three times.Utilize the DHI system, gathered the milk sample every 7 days 1 time, measure milk elements.

The result that feeds and milk elements measurement result are as follows: experimental group milk cow common property milk 45690kg, control group milk cow common property milk 42787kg, the butterfat percnetage of experimental group and protein ratio are respectively 3.5 ± 0.1 and 2.9 ± 0.2, the butterfat percnetage of control group and protein ratio 3.2 ± 0.1 and 2.5 ± 0.2.Show and use liquid animal feeds additive finished product feeding cow of the present invention, fat and the protein content that improves in the cow's milk all had facilitation preferably.

Claims (7)

1. animal feed additive is characterized in that: its main component is the solid residue after microbial grease extracts, i.e. microbe oil fermentation bacterium slag.

2. animal feed additive according to claim 1 is characterized in that: described microbe oil fermentation bacterium slag gets additive product preferably by hydrolysis process, improves its bioavilability.

3. animal feed additive according to claim 2, its feature also is: described hydrolysis process mode is enzyme hydrolysis, acid hydrolysis or their combination, concrete hydrolysising condition is:

(1) enzyme hydrolysis: microbe oil fermentation bacterium slag is mixed with water, make its total solid concentration reach 10%～90% (w/w), add hydrolase to total enzyme concentration and reach 0.005%～3% (w/w), be incubated 10 minutes～40 hours down at 20 ℃～90 ℃;

(2) acid hydrolysis: microbe oil fermentation bacterium slag is mixed with water, make its total solid concentration reach 10%～90% (w/w),, be incubated 10 minutes～24 hours down, be neutralized to pH 5.5～7.5 with alkali again at 20 ℃～90 ℃ with acid for adjusting pH 1.0～5.0.

4. animal feed additive according to claim 2, its feature also is: described hydrolase is one or more the combination in dextranase, mannase, glucomannan enzyme, cellulase, glusulase or the chitinase; Used acid is inorganic acid or organic acid; Used alkali is inorganic base or organic base.

5. animal feed additive according to claim 2, its feature also is: described microbe oil fermentation bacterium slag is directly made total solid concentration and is the liquid additive finished product of 10%～60% (w/w) or make the solid additives finished product 20 ℃～100 ℃ dryings after hydrolysis process.

6. animal feed additive according to claim 1 and 2, its feature also is: described microorganism is for surpassing the eucaryon or the prokaryotic micro-organisms of its biological total amount 20% (w/w) at the intracellular accumulation grease.

7. the application of the described animal feed additive of claim 1 is characterized in that: described additive is used for animal-breeding feed as mycoprotein, presses solid content meter, and additive amount can be mixes 0.1%～20% of back feed gross weight.