CN101100691A - Method for extracting small peptides from pine pollen by using enzyme - Google Patents
Method for extracting small peptides from pine pollen by using enzyme Download PDFInfo
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- CN101100691A CN101100691A CNA2007100580280A CN200710058028A CN101100691A CN 101100691 A CN101100691 A CN 101100691A CN A2007100580280 A CNA2007100580280 A CN A2007100580280A CN 200710058028 A CN200710058028 A CN 200710058028A CN 101100691 A CN101100691 A CN 101100691A
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Abstract
Peptide with small molecular weight is extracted from Chinese pine pollen with enzyme, carried out by: extracting non-protein components from pollen, adding the extracted pollen into container with agitator, regulating pH to 7-9 in drinking water and stirring for 2-4 hours to mixed pollen liquid, adding enzyme into the liquid and stirring 2-16 hours, and separating residue to obtain nutrient liquid of small weight peptide, which is easily absorptive by human beings and water soluble.
Description
Technical field
The invention belongs to molecular bioengineering and biochemical engineering field, especially a kind of method of from Chinese pine pollen, extracting little peptide with enzyme.
Background technology
Pollen Pini contains whole nutritive ingredients of needed by human body, seed amino acid surplus the multiple proteins, 20 (comprise needed by human 8 kinds), 14 kinds of VITAMIN, 30 multi mineral prime elements, 11 kinds of fat, nearly hundred kinds of enzymes and coenzyme and nucleic acid, flavones, monose, polysaccharide etc. are wherein arranged, kind surplus total amount reaches 200; And collocation rationally, and the nutrition of can all replenish, balancing human body is required have the title of " green gold ".Studies show that in recent years, hydrolysis end product major part is the little peptide that 2 or 3 amino-acid residues are formed under the digestive ferment effect of protein in animal digestive tract, it is fast that the absorption of peptide has speed, it is low to consume energy, be difficult for transporting between the saturated and various peptides characteristics of uncontested property and inhibition, the bio-enzyme engineering working method is under mild reaction conditions, hydrolysis generates materials such as polypeptide and amino acid, have very high nutritive value and functional performance, and be easy to absorption of human body and digestion, it is controlled to have reaction, side reaction is few, good product quality and do not destroy characteristics such as amino acid, biological enzyme will be that the plant protein resource development and use are the most effective, the most promising working method.
Up to now, the method for extraction nutrition roughly has two classes from pollen.The first kind is to extract nutrition with the method for physics from pollen.Be characterized in keeping the molecular structure of pollen original nutrient contents constant, but the pollen extracting liquid filtration difficulty that obtains, product yield is low.If use its synthetic beverage, because it is water-soluble not high, often in the thermal sterilization process, can produce proteinic solidification phenomenon, have a strong impact on the outward appearance and the quality of product.Thereby, can only make solid products such as cake of it.Second class is to make the protein transduction in the pollen become the multiple amino acids that easily is absorbed by the body with fermentation method.This method during the fermentation, the part nutrition that breeding of microorganism and growth all need to consume pollen, thereby yield is low, and the production cycle long (the solid fermentation cycle is 10-20 days, and the liquid fermenting cycle is 4-5 days).
Summary of the invention
The object of the present invention is to provide a kind of novel method of from Chinese pine pollen, extracting little peptide.This method is not only with short production cycle, the product yield height, and also the nutrition of extracting solution is the small-molecular peptides that is absorbed by the body easily, the active small molecular peptide of gained has characteristics such as the water-soluble height of nutritive medium, Heat stability is good.
The present invention solves its technical problem and is achieved through the following technical solutions:
A kind ofly extract the method for little peptide with enzyme from Chinese pine pollen, it is characterized in that: the method for extracting little peptide may further comprise the steps:
(1). go out the composition of nonprotein in the Chinese pine pollen with solvent extraction;
(2). the Chinese pine pollen after will extracting is put into the container that has agitator, be 7～9 with 20～70 ℃ tap water regulator solution pH values after, stirred 2～4 hours, form the Chinese pine pollen mixed solution;
(3). in the Chinese pine pollen mixed solution, add enzyme, fully stirred 2～8 hours;
(4). the material slag is removed in centrifugation while hot, promptly obtains small peptide nutrient liquid.
And the weight ratio of described Chinese pine pollen and tap water is 1～5: 1～15.
And the described enzyme that adds in the Chinese pine pollen mixed solution is a kind of or wherein several mixture in papoid, Sumizyme MP, the trypsinase.
And the weight ratio of the addition of described enzyme and Chinese pine pollen (butt) is 0.2～0.4: 50～150.
And described little peptide is the active small molecular peptide that easily is absorbed by the body that 2～8 amino acid are formed.
Advantage of the present invention and beneficial effect are:
1. the present invention is the katalysis that utilizes enzyme, makes the protein transduction in the Chinese pine pollen turn to the small-molecular peptides that easily is absorbed by the body.Enzyme reaction rate is fast, is generally about 3 hours, has greatly shortened the production cycle, has improved production efficiency.The nutrition of not loss Chinese pine pollen in the enzyme-catalyzed reaction, and it is controlled to have a reaction, side reaction is few, yield height, good product quality and do not destroy characteristics such as amino acid.
2. the Chinese pine pollen extracting solution separate easily that adopts the present invention to make, water-soluble height does not destroy the nutrition of pollen, and does not exist pollen extracting liquid to be subjected to the protein coagulated phenomenon of thermogenesis.This beverage has micronutrient elements such as the active small peptide of the absorption of human body of being easy to and some mineral substance.
3. the Chinese pine pollen extracting solution of taking method of the present invention to make not only contains activated small-molecular peptides, also is rich in trace elements such as some mineral substance.This extracting solution can be used for directly making up oil Pollen Pini beverage and other purposes; The small peptide nutrient liquid of gained can be added in the preparations such as makeup, especially in the makeup of emulsion form, is beneficial to skin absorption etc.; The filter residue of gained can make the coarse-fibred diet food of high-content etc.
Embodiment
The invention will be further described below by specific embodiment, and following examples are descriptive, is not determinate, can not limit protection scope of the present invention with this.
Embodiment 1:
This extracts the method for little peptide with enzyme from Chinese pine pollen, be the catalyzed reaction effect that utilizes enzyme, makes the protein transduction in the Chinese pine pollen become the active small molecular peptide that easily is absorbed by the body, and its extracting method may further comprise the steps:
(1). extract the composition of nonprotein in the Chinese pine pollen with solvent (ethanol or water or edible solvent oil);
(2). Chinese pine pollen 100 grams after will extracting are put into the container of a belt stirrer, under 45 ℃ of temperature condition, solution are adjusted to pH=7, soaking and stirring 3 hours with 1000 drinking public water supplies that restrain.
(3). in above-mentioned solution, add 3 gram papoids, fully stirred 3 hours;
(4). centrifugation while hot, remove the material slag, make small peptide nutrient liquid.
Embodiment 2:
PH value in the processing step among the embodiment 1 (2) is risen to 9, and make the kind of enzyme into Sumizyme MP by papoid and get final product.
Other working method are with embodiment 1.
Embodiment three
PH value in the processing step among the embodiment 1 (2) is adjusted to 7.5, and makes the kind of enzyme into mixing protease (that is: the mixed enzyme of papoid and Sumizyme MP is used by 1: 1 amount) by papoid.Both interpolations add papoid again for adding the basic protein enzyme reaction earlier in proper order after 1 hour, two kinds of common reactions of enzyme separated after 2 hours, obtained small peptide nutrient liquid.
Other working method are with embodiment 1.
Claims (5)
1. one kind is extracted the method for little peptide with enzyme from Chinese pine pollen, and it is characterized in that: the method for extracting little peptide may further comprise the steps:
(1). go out the composition of nonprotein in the Chinese pine pollen with solvent extraction;
(2). the Chinese pine pollen after will extracting is put into the container that has agitator, be 7～9 with 20～70 ℃ tap water regulator solution pH values after, stirred 2～4 hours, form the Chinese pine pollen mixed solution;
(3). in the Chinese pine pollen mixed solution, add enzyme, fully stirred 2～8 hours;
(4). the material slag is removed in centrifugation while hot, promptly obtains small peptide nutrient liquid.
2. according to claim 1ly extract the method for little peptide with enzyme from Chinese pine pollen, it is characterized in that: the weight ratio of described Chinese pine pollen and tap water is 1～5: 1～15.
3. according to claim 1ly extract the method for little peptide with enzyme from Chinese pine pollen, it is characterized in that: the described enzyme that adds in the Chinese pine pollen mixed solution is a kind of or wherein several mixture in papoid, Sumizyme MP, the trypsinase.
4. describedly extract the method for little peptide with enzyme from Chinese pine pollen according to claim 1 or 3, it is characterized in that: the weight ratio of the addition of described enzyme and Chinese pine pollen (butt) is 0.2～0.4: 50～150.
5. according to claim 1ly extract the method for little peptide with enzyme from Chinese pine pollen, it is characterized in that: described little peptide is the active small molecular peptide that easily is absorbed by the body that 2～8 amino acid are formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100580280A CN101100691A (en) | 2007-07-13 | 2007-07-13 | Method for extracting small peptides from pine pollen by using enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100580280A CN101100691A (en) | 2007-07-13 | 2007-07-13 | Method for extracting small peptides from pine pollen by using enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101100691A true CN101100691A (en) | 2008-01-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CNA2007100580280A Pending CN101100691A (en) | 2007-07-13 | 2007-07-13 | Method for extracting small peptides from pine pollen by using enzyme |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229973A (en) * | 2011-06-15 | 2011-11-02 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
| CN102659917A (en) * | 2012-05-24 | 2012-09-12 | 烟台新时代健康产业有限公司 | Method for separating and preparing crude protein and enzymolysis peptide from pollen pini |
| CN107574215A (en) * | 2017-07-25 | 2018-01-12 | 赵德润 | A kind of preparation method of pollen pini active peptide |
| CN107988299A (en) * | 2017-12-01 | 2018-05-04 | 烟台新时代健康产业有限公司 | A kind of method that anti-oxidant pollen pini peptide is prepared using the pollen pini dregs of rice |
| CN109694895A (en) * | 2019-03-06 | 2019-04-30 | 河北神顺健康科技有限公司 | Preserved egg active peptide, the composition comprising it, agaric slice and its preparation process |
-
2007
- 2007-07-13 CN CNA2007100580280A patent/CN101100691A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229973A (en) * | 2011-06-15 | 2011-11-02 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
| CN102229973B (en) * | 2011-06-15 | 2013-05-01 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
| CN102659917A (en) * | 2012-05-24 | 2012-09-12 | 烟台新时代健康产业有限公司 | Method for separating and preparing crude protein and enzymolysis peptide from pollen pini |
| CN107574215A (en) * | 2017-07-25 | 2018-01-12 | 赵德润 | A kind of preparation method of pollen pini active peptide |
| CN107988299A (en) * | 2017-12-01 | 2018-05-04 | 烟台新时代健康产业有限公司 | A kind of method that anti-oxidant pollen pini peptide is prepared using the pollen pini dregs of rice |
| CN107988299B (en) * | 2017-12-01 | 2021-06-01 | 烟台新时代健康产业有限公司 | Method for preparing antioxidant pollen pini peptide by utilizing pollen pini dregs |
| CN109694895A (en) * | 2019-03-06 | 2019-04-30 | 河北神顺健康科技有限公司 | Preserved egg active peptide, the composition comprising it, agaric slice and its preparation process |
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Open date: 20080109 |