CN106047679B - Microorganism concn detection device and its application method in a kind of water of electrical control - Google Patents
Microorganism concn detection device and its application method in a kind of water of electrical control Download PDFInfo
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- CN106047679B CN106047679B CN201610376305.1A CN201610376305A CN106047679B CN 106047679 B CN106047679 B CN 106047679B CN 201610376305 A CN201610376305 A CN 201610376305A CN 106047679 B CN106047679 B CN 106047679B
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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Abstract
The invention discloses microorganism concn detection device and its application methods in a kind of water of electrical control, including culture dish, inlet channel, manually-operated gate, water sample container, inlet channel interface, water sample, push-pull device at fixed, chassis, outgassing column, nutrient solution, magnifying glass, air filtration film, pure water, outlet conduit, electrically operated valve, outlet conduit interface, aperture and control device, microorganism concn detection device and its application method are easy to operate in a kind of water of electrical control, it is not easy by environmental pollution, microorganism concn dilution changes in gradient, it is convenient to count, the concentration drawn more has reliability, instrument is reusable, it is good for the environment；By control device, the electrified accurate water yield for controlling water sample container is realized.
Description
Technical field
The present invention relates to electrical and microorganism metering fields, are specially microorganism concn detection in a kind of water of electrical control
Device and its application method.
Background technology
Water is Source of life, is the natural surroundings that microorganism is widely present, all contains in various natural waters a certain number of
Microorganism.Microorganism concn detects, and has extremely important meaning on the safety of water source and control infectious disease is ensured, while is also to comment
The important indicator of valency water quality condition.
The method of microorganism concn detection, there is colony counting method, i.e., therein micro- by sample to be tested after appropriate dilution
Biology is fully dispersed into individual cells, a certain amount of dilution sample liquid is taken to be applied on tablet, by culture, by each unicellular life
Length breeds and forms macroscopic bacterium colony, i.e. unicellular, the statistics clump count that a single bacterium colony should be represented in raw sample,
It can be conversed in sample according to its extension rate and sampling inoculum concentration and contain bacterium number.But many detecting instruments are all disposable
It uses, gives it up after use, cause to waste.
The shortcomings that above method is：Time-consuming, cumbersome to be vulnerable to environmental pollution, and obtained result differs and fixes
Really, instrument is disposable, causes to waste.
The content of the invention
It is an object of the invention to provide microorganism concn detection device and its application method in a kind of water of electrical control,
To solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical solution：Microorganism concn is examined in a kind of water of electrical control
Device is surveyed, including culture dish, water sample container, chassis and control device, the position at four angles in chassis is provided with column, uses
In being provided with one or more culture dishes in support chassis, the chassis, inlet channel is connected at the top of the culture dish,
Manually-operated gate is provided between culture dish and inlet channel, the manually-operated gate is close at the top of culture dish, and culture dish bottom connects
Then outlet conduit is provided with electrically operated valve between culture dish and outlet conduit, the electrically operated valve is located inside chassis, tightly
Culture dish bottom is pasted, the culture dish includes outgassing column, nutrient solution, magnifying glass and aperture, and the outgassing column includes air filtration
Film and pure water, the magnifying glass are located at the top of culture dish, and convenient for observing clump count, the aperture is for exclusion volatilization
Moisture and injection nutrient solution, the outlet conduit interface of the outlet conduit are used to connect suction pump or container；The control
Device processed connects water sample container by conducting wire, and then is accurately controlled the water yield of water sample container；
The water sample container includes inlet channel interface, water sample and push-pull device at fixed, wherein, the inlet channel interface
For connecting inlet channel, water sample is positioned in water sample container, under the action of push-pull device at fixed is controlled by control device, water sample
It is transferred to by water sample container by inlet channel in each culture dish.
Preferably, the culture dish volume is 1mL or 10mL.
Preferably, the air filtration film is hydrophobic permeable membrane, can only pass through gas, it is impossible to pass through liquid.
Preferably, the manually-operated gate is Manual check valve.
Preferably, the electrically operated valve is motorized non-return valve.
Microorganism concn detection device in a kind of water of electrical control, application method are：
Chassis level is positioned on platform by the first step, it is ensured that the aperture on culture dish is sealing, manually-operated gate and electricity
Movable valve is to close, in case culture dish is contaminated；
Second step, the upper inlet channel of inlet channel interface connection by water sample container；
3rd step, the push-pull device at fixed for opening water sample container, the water sample after diluting no dilution or by a certain percentage are placed
It is put into water sample container, then by push-pull device at fixed in water sample container, opens manually-operated gate, and push-and-pull dress is controlled by control device
It puts and pressurizes to water sample, the water sample of certain volume is made to be flowed by inlet channel in each culture dish；
4th step, one or more culture dishes are one group, and the nutrient solution volume in each group culture dish changes in gradient respectively,
Scope is 0 ~ 90%, then the water sample flowed into each group culture dish is also had nothing in common with each other, that is, the water sample flowed into each culture dish is each
Nutrient solution in group is diluted by different proportion, when water sample is flowed into each culture dish and is full of, closes manually-operated gate；
5th step, slight wobble chassis make the nutrient solution in each culture dish be sufficiently mixed with water sample, contain in culture dish
Air outgassing column is entered by air filtration film, then the external world is drained by pure water and air filtration film respectively；
6th step, when in culture dish microorganism formed bacterium colony can be seen by the magnifying glass on culture dish when, then select
The clear denumerable culture dish of bacterium colony is selected, counts respectively, is finally converted into concentration；
7th step opens electrically operated valve, and the nutrient solution in culture dish is discharged with water sample by outlet conduit；
Water sample in water sample container is changed into pure water by the 8th step, opens manually-operated gate, is closed electrically operated valve, is passed through control
Device control push-pull device at fixed pressure processed, makes the pure water of certain volume be flowed by inlet channel in each culture dish, closes hand
Movable valve, slight wobble culture dish connect upper suction pump or container for several times, by outlet conduit interface, open electrically operated valve, will train
The water supported in ware is discharged by outlet conduit；
9th step repeats the 8th step five times, afterwards removes water sample container, and inlet channel is connected upper clean fan, is beaten
Manually-operated gate and electrically operated valve are opened, while unseals aperture, fresh air by inlet channel, is made inlet channel and training by clean fan
The moisture evaporation in ware is supported, is excluded from aperture or outlet conduit, the time continues 20 minutes；
It is out-of-blast in tenth step, inlet channel and culture dish during water stain-free, manually-operated gate and electrically operated valve are closed, it will
The nutrient solution of a certain amount of volume is sent by aperture into culture dish, and sealed aperture, in case next time is used.
Preferably, the culture dish is one group two-by-two.
Preferably, the medium volume gradient scope in each group culture dish is 10% ~ 80%.
Compared with prior art, the beneficial effects of the invention are as follows：Microorganism concn detection device and its make in the water
Multiple culture dishes are used with method, facilitate the dilution water sample of disposable various concentration gradient, and culture dish includes hydrophobic, air-permeability
Film, so will be free from gas in culture dish, valve used is all check-valves, will not be polluted by external microbe, culture dish
Top is equipped with magnifying glass, can directly select the easily several culture dish of cleaning and count bacterium colony, need not be put into micro- Microscopic observation again, and one
Secondary experiment is finished, and the device can be cleaned, and adds nutrient solution, in case next time uses, microorganism concn is examined in the water
It surveys device and its application method is easy to operate, be not easy by environmental pollution, microorganism concn dilution changes in gradient, and it is convenient to count,
The concentration drawn more has reliability, and instrument is reusable, is good for the environment；By control device, realize electrified accurate
Control the water yield of water sample container.
Description of the drawings
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is chassis-bottom structure schematic diagram of the present invention；
Fig. 3 is the structure diagram of culture dish of the present invention；
Fig. 4 is the structure diagram of outgassing column of the present invention；
Fig. 5 is the top view of the present invention；
Fig. 6 is the sectional view at Fig. 5 A-A；
Fig. 7 is the sectional view at Fig. 5 B-B.
In figure：1st, culture dish, 2, inlet channel, 3, manually-operated gate, 4, water sample container, 5, inlet channel interface, 6, water sample,
7th, push-pull device at fixed, 8, chassis, 9, outgassing column, 10, nutrient solution, 11, magnifying glass, 12, air filtration film, 13, pure water, 14, outlet pipe
Road, 15, electrically operated valve, 16, outlet conduit interface, 17, aperture, 18, control device.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work
Embodiment belongs to the scope of protection of the invention.
Embodiment 1：
- 7 are please referred to Fig.1, the present invention provides a kind of technical solution：Microorganism concn detection dress in a kind of water of electrical control
It puts, including culture dish 1, water sample container 4, chassis 8 and control device 18, it is characterized in that：The position at 8 four angles in chassis is provided with vertical
Column is used to support chassis 8, and one or more culture dishes 1 are provided on chassis 8, and 1 top of culture dish is connected to inlet channel 2, trains
It supports and manually-operated gate 3 is provided between ware 1 and inlet channel 2, manually-operated gate 3 is close to 1 top of culture dish, the connection of 1 bottom of culture dish
Outlet conduit 14, electrically operated valve 15 is provided between culture dish 1 and outlet conduit 14, electrically operated valve 15 is located inside chassis 8,
It is close to 1 bottom of culture dish, culture dish 1 includes outgassing column 9, nutrient solution 10, magnifying glass 11 and aperture 17, and outgassing column 9 includes air filtration
Film 12 and pure water 13, magnifying glass 11 are located at 1 top of culture dish, convenient for observing clump count, the water that aperture 17 volatilizees for exclusion
Divide and injection nutrient solution 10, the outlet conduit interface 16 of outlet conduit 14 are used to connect suction pump or container；Control device 18
Water sample container 4 is connected by conducting wire, and then is accurately controlled the water yield of water sample container 4；
Water sample container 4 includes inlet channel interface 5, water sample 6 and push-pull device at fixed 7, wherein, inlet channel interface 5 is used to connect
Waterpipe 2 is tapped into, water sample 6 is positioned in water sample container 4, under the action of push-pull device at fixed 7 is controlled by control device 18, water sample
6 are transferred to by inlet channel 2 in each culture dish 1 by water sample container 4.
Microorganism concn detection device operating method is in a kind of water of electrical control：
Chassis 8 is placed horizontally on platform by the first step, it is ensured that the aperture 17 on culture dish 1 is to seal, Manual check
Valve 3 is to close with motorized non-return valve 15, in case culture dish 1 is contaminated；
The inlet channel interface 5 of water sample container 4 is connected upper inlet channel 2 by second step；
3rd step, the push-pull device at fixed 7 for opening water sample container 4, the water sample 6 after diluting no dilution or by a certain percentage are put
It is placed in water sample container 4, then push-pull device at fixed 7 is put into water sample container 4, open manually-operated gate 3, and pass through control device 18 and control
Push-pull device at fixed 7 processed pressurizes to water sample 6, and the water sample 6 of certain volume is made to be flowed by inlet channel 2 in each culture dish 1；
The culture dish 1 of 10 10mL is shared in 4th step, chassis 8, is two-by-two one group, totally 5 groups, in every group of culture dish 1
Nutrient solution 10 is respectively 0mL, 2mL, 4mL, 6mL, 8mL, then the water sample 6 for flowing into each culture dish 1 is respectively 10mL,
8mL, 6mL, 4mL, 2mL when water sample 6 is flowed into each culture dish 1 and is full of, close Manual check valve 3；
5th step, slight wobble chassis 8 make the nutrient solution 10 in each culture dish 1 be sufficiently mixed with water sample 6, culture dish 1
In the air that contains outgassing column 9 is entered by air filtration film 12, then the external world is drained by pure water 13 and air filtration film 12 respectively；
6th step, when in culture dish 1 microorganism formed bacterium colony can be seen by the magnifying glass 11 on culture dish 1 when,
The clear denumerable culture dish 1 of bacterium colony is then selected, counts respectively, is finally converted into concentration；
7th step opens motorized non-return valve 15, and the nutrient solution 10 in culture dish 1 is discharged with water sample 6 by outlet conduit 14；
Water sample 6 in water sample container 4 is changed into pure water by the 8th step, opens manually-operated gate 3, closes electrically operated valve 15, is led to
Crossing control device 18 controls push-pull device at fixed 7 to press, and the pure water of certain volume is made to flow into each culture dish 1 by inlet channel 2
In, manually-operated gate 3 is closed, slight wobble culture dish 1 connects upper suction pump or container for several times, by outlet conduit interface 16, opens
Electrically operated valve 15 discharges the water in culture dish 1 by outlet conduit 14；
9th step repeats the 8th step five times, afterwards removes water sample container 4, inlet channel 2 is connected upper clean fan,
Open Manual check valve 3 and motorized non-return valve 15, while unseal aperture 17, clean fan by fresh air by inlet channel 2, make into
Moisture evaporation in waterpipe 2 and culture dish 1 is excluded from aperture 17 or outlet conduit 14, and the time continues 20 minutes；
It is out-of-blast in tenth step, inlet channel 2 and culture dish 1 during water stain-free, it closes Manual check valve 3 and stops with electronic
Return valve 15, the nutrient solution 10 of a certain amount of volume sent by aperture 17 into culture dish 1, and sealed aperture 17, in case under take second place
With.
Embodiment 2：
- 7 are please referred to Fig.1, the present invention provides a kind of technical solution：Microorganism concn detection dress in a kind of water of electrical control
It puts, including culture dish 1, water sample container 4, chassis 8 and control device 18, it is characterized in that：The position at 8 four angles in chassis is provided with vertical
Column is used to support chassis 8, and one or more culture dishes 1 are provided on chassis 8, and 1 top of culture dish is connected to inlet channel 2, trains
It supports and manually-operated gate 3 is provided between ware 1 and inlet channel 2, manually-operated gate 3 is close to 1 top of culture dish, the connection of 1 bottom of culture dish
Outlet conduit 14, electrically operated valve 15 is provided between culture dish 1 and outlet conduit 14, electrically operated valve 15 is located inside chassis 8,
It is close to 1 bottom of culture dish, culture dish 1 includes outgassing column 9, nutrient solution 10, magnifying glass 11 and aperture 17, and outgassing column 9 includes air filtration
Film 12 and pure water 13, magnifying glass 11 are located at 1 top of culture dish, convenient for observing clump count, the water that aperture 17 volatilizees for exclusion
Divide and injection nutrient solution 10, the outlet conduit interface 16 of outlet conduit 14 are used to connect suction pump or container；Control device 18
Water sample container 4 is connected by conducting wire, and then is accurately controlled the water yield of water sample container 4；
Water sample container 4 includes inlet channel interface 5, water sample 6 and push-pull device at fixed 7, wherein, inlet channel interface 5 is used to connect
Waterpipe 2 is tapped into, water sample 6 is positioned in water sample container 4, under the action of push-pull device at fixed 7 is controlled by control device 18, water sample
6 are transferred to by inlet channel 2 in each culture dish 1 by water sample container 4.
Microorganism concn detection device operating method is in a kind of water of electrical control：
Chassis 8 is placed horizontally on platform by the first step, it is ensured that the aperture 17 on culture dish 1 is to seal, Manual check
Valve 3 is to close with motorized non-return valve 15, in case culture dish 1 is contaminated；
The inlet channel interface 5 of water sample container 4 is connected upper inlet channel 2 by second step；
3rd step, the push-pull device at fixed 7 for opening water sample container 4, the water sample 6 after diluting no dilution or by a certain percentage are put
It is placed in water sample container 4, then push-pull device at fixed 7 is put into water sample container 4, open manually-operated gate 3, and pass through control device 18 and control
Push-pull device at fixed 7 processed pressurizes to water sample 6, and the water sample 6 of certain volume is made to be flowed by inlet channel 2 in each culture dish 1；
The culture dish 1 of 20 1mL is shared in 4th step, chassis 8, is two-by-two one group, totally 10 groups, in every group of culture dish 1
Nutrient solution 10 respectively 0mL, 0.1mL, 0.2mL, 0.3mL, 0.4mL, 0.5mL, 0.6mL, 0.7mL, 0.8mL,
0.9mL then flows into water sample 6 respectively 1mL, 0.9mL, 0.8mL, 0.7mL, 0.6mL, the 0.5mL of each culture dish 1,
0.4mL, 0.3mL, 0.2mL, 0.1mL when water sample 6 is flowed into each culture dish 1 and is full of, close Manual check valve
3；
5th step, slight wobble chassis 8 make the nutrient solution 10 in each culture dish 1 be sufficiently mixed with water sample 6, culture dish 1
In the air that contains outgassing column 9 is entered by air filtration film 12, then the external world is drained by pure water 13 and air filtration film 12 respectively；
6th step, when in culture dish 1 microorganism formed bacterium colony can be seen by the magnifying glass 11 on culture dish 1 when,
The clear denumerable culture dish 1 of bacterium colony is then selected, counts respectively, is finally converted into concentration；
7th step opens motorized non-return valve 15, and the nutrient solution 10 in culture dish 1 is discharged with water sample 6 by outlet conduit 14；
Water sample 6 in water sample container 4 is changed into pure water by the 8th step, opens manually-operated gate 3, closes electrically operated valve 15, is led to
Crossing control device 18 controls push-pull device at fixed 7 to press, and the pure water of certain volume is made to flow into each culture dish 1 by inlet channel 2
In, manually-operated gate 3 is closed, slight wobble culture dish 1 connects upper suction pump or container for several times, by outlet conduit interface 16, opens
Electrically operated valve 15 discharges the water in culture dish 1 by outlet conduit 14；
9th step repeats the 8th step five times, afterwards removes water sample container 4, inlet channel 2 is connected upper clean fan,
Open Manual check valve 3 and motorized non-return valve 15, while unseal aperture 17, clean fan by fresh air by inlet channel 2, make into
Moisture evaporation in waterpipe 2 and culture dish 1 is excluded from aperture 17 or outlet conduit 14, and the time continues 20 minutes；
It is out-of-blast in tenth step, inlet channel 2 and culture dish 1 during water stain-free, it closes Manual check valve 3 and stops with electronic
Return valve 15, the nutrient solution 10 of a certain amount of volume sent by aperture 17 into culture dish 1, and sealed aperture 17, in case under take second place
With.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, technique according to the invention scheme and its
It improves design and is subject to equivalent substitution or change, should all cover within the scope of the present invention.
Claims (8)
1. a kind of microorganism concn detection device in water of electrical control, including culture dish（1）, water sample container（4）, chassis（8）
And control device（18）, it is characterized in that：The chassis（8）The position at four angles is provided with column, is used to support chassis（8）,
The chassis（8）On be provided with one or more culture dishes（1）, the culture dish（1）Top is connected to inlet channel
（2）, culture dish（1）With inlet channel（2）Between be provided with manually-operated gate（3）, the manually-operated gate（3）It is close to culture dish
（1）Top, culture dish（1）Bottom is connected to outlet conduit（14）, culture dish（1）With outlet conduit（14）Between be provided with it is electronic
Valve（15）, the electrically operated valve（15）Positioned at chassis（8）Culture dish is close in inside（1）Bottom, the culture dish（1）
Including outgassing column（9）, nutrient solution（10）, magnifying glass（11）And aperture（17）, the outgassing column（9）Including air filtration film（12）With
Pure water（13）, the magnifying glass（11）Positioned at culture dish（1）Top, convenient for observing clump count, the aperture（17）With
In the moisture and injection nutrient solution that exclude volatilization（10）, the outlet conduit（14）Outlet conduit interface（16）For even
Connect suction pump or container；The control device（18）Water sample container is connected by conducting wire（4）, and then it is accurately controlled water sample appearance
Device（4）Water yield；
The water sample container（4）Including inlet channel interface（5）, water sample（6）And push-pull device at fixed（7）, wherein, the water inlet
Pipe joint（5）For connecting inlet channel（2）, water sample（6）It is positioned over water sample container（4）In, passing through control device（18）
Control push-pull device at fixed（7）Under the action of, water sample（6）By water sample container（4）By inlet channel（2）It is transferred to each culture dish
（1）In.
2. microorganism concn detection device in a kind of water of electrical control according to claim 1, it is characterised in that：It is described
Culture dish（1）Volume is 1mL or 10mL.
3. microorganism concn detection device in a kind of water of electrical control according to claim 1, it is characterised in that：It is described
Air filtration film（12）For hydrophobic permeable membrane, gas can only be passed through, it is impossible to pass through liquid.
4. microorganism concn detection device in a kind of water of electrical control according to claim 1, it is characterised in that：It is described
Manually-operated gate（3）For Manual check valve.
5. microorganism concn detection device in a kind of water of electrical control according to claim 1, it is characterised in that：It is described
Electrically operated valve（15）For motorized non-return valve.
6. microorganism concn detection device in a kind of water of electrical control according to claim 1, application method are：
The first step, by chassis（8）It is placed horizontally on platform, it is ensured that culture dish（1）On aperture（17）It is to seal, hand-operated valve
Door（3）With electrically operated valve（15）It is to close, in order to avoid culture dish（1）It is contaminated；
Second step, by water sample container（4）Inlet channel interface（5）Inlet channel in connection（2）；
3rd step opens water sample container（4）Push-pull device at fixed（7）, the water sample after diluting no dilution or by a certain percentage（6）
It is positioned over water sample container（4）In, then by push-pull device at fixed（7）It is put into water sample container（4）In, open manually-operated gate（3）, and pass through control
Device processed（18）Control push-pull device at fixed（7）To water sample（6）Pressurization makes the water sample of certain volume（6）Pass through inlet channel（2）It flows into
To each culture dish（1）In；
4th step, one or more culture dishes（1）For one group, the nutrient solution in each group culture dish（10）Volume becomes in gradient respectively
Change, scope is 0 ~ 90%, then flows into each group culture dish（1）In water sample（6）Also have nothing in common with each other, that is, flow into each culture dish（1）In
Water sample（6）By the nutrient solution in each group（10）It is diluted by different proportion, works as water sample（6）It is flowed into each culture dish（1）In
And when being full of, manually-operated gate is closed（3）；
5th step, slight wobble chassis（8）, make each culture dish（1）In nutrient solution（10）With water sample（6）It is sufficiently mixed, trains
Support ware（1）In the air that contains pass through air filtration film（12）Into outgassing column（9）, then pass through pure water respectively（13）With air filtration film
（12）Drain into the external world；
6th step treats culture dish（1）In microorganism formed bacterium colony can pass through culture dish（1）On magnifying glass（11）It sees
When, then select the clear denumerable culture dish of bacterium colony（1）, count respectively, be finally converted into concentration；
7th step opens electrically operated valve（15）, culture dish（1）In nutrient solution（10）With water sample（6）Pass through outlet conduit（14）
Discharge；
8th step, by water sample container（4）In water sample（6）It changes pure water into, opens manually-operated gate（3）, close electrically operated valve
（15）, pass through control device（18）Control push-pull device at fixed（7）Pressure makes the pure water of certain volume pass through inlet channel（2）Stream
Enter each culture dish（1）In, close manually-operated gate（3）, slight wobble culture dish（1）For several times, by outlet conduit interface（16）Even
Suction pump or container are connected, opens electrically operated valve（15）, by culture dish（1）In water pass through outlet conduit（14）Discharge；
9th step repeats the 8th step five times, afterwards by water sample container（4）It removes, by inlet channel（2）Clean fan in connection,
Open manually-operated gate（3）With electrically operated valve（15）, while unseal aperture（17）, fresh air passes through inlet channel by clean fan（2）,
Make inlet channel（2）And culture dish（1）In moisture evaporation, from aperture（17）Or outlet conduit（14）Middle exclusion, time hold
It is 20 minutes continuous；
Tenth step, inlet channel（2）And culture dish（1）It is out-of-blast during middle water stain-free, close manually-operated gate（3）With motor-driven valve
Door（15）, by the nutrient solution of a certain amount of volume（10）Pass through aperture（17）It send to culture dish（1）In, and sealed aperture（17）, with
Standby next time is used.
7. the application method of microorganism concn detection device in water according to claim 6, it is characterised in that：The training
Support ware（1）It is one group two-by-two.
8. the application method of microorganism concn detection device in water according to claim 7, it is characterised in that：Described is each
Group culture dish（1）In culture solution（10）Volume gradient scope is 10% ~ 80%.
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| CN111088156B (en) * | 2020-01-06 | 2021-03-30 | 山东省海洋资源与环境研究院 | Coliform group testing piece for marine environment monitoring and accessory device used in cooperation with coliform group testing piece |
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| CN102449460B (en) * | 2009-04-03 | 2014-11-12 | 3M创新有限公司 | Microorganism concentration process and device |
| CN102816683B (en) * | 2012-08-01 | 2013-10-09 | 江苏嘉语生物医药技术有限公司 | Biochemical culture and detection device as well as detection method thereof |
| CN102796660B (en) * | 2012-08-27 | 2015-08-05 | 中国科学院长春应用化学研究所 | For proofing unit and the on-line water quality monitoring method of monitoring water quality on line |
| CN103940709A (en) * | 2014-05-06 | 2014-07-23 | 南京中科神光科技有限公司 | Real-time microbial particle counter |
| CN205139122U (en) * | 2015-11-26 | 2016-04-06 | 边瑞玲 | Little biological detection device of food |
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