CN204401001U - Airborne particulate matter collection device in cell culture medium - Google Patents

Abstract

The utility model discloses a device for collecting atmospheric particles in a cell culture medium. At present, the main reason for the in-depth research on the toxicity of particulate matter is that the extraction of PM2.5 particulate matter is difficult and the toxicity evaluation is not comprehensive. The cylinder body of the utility model is divided into three layers; the membrane fixing frame is nested on the upper layer of the cylinder body; multiple filter membranes are layered on the membrane fixing frame; the bottom of the upper partition and the top of the lower partition are fixed with ultraviolet light Bacteria lamp; the air hose is fixed on the middle layer of the cylinder through an iron ring; the upper end of the air hose extends into the bottom of the film holder, and the lower end passes through the lower partition; the air hose is equipped with a flow meter and an adjustable pump The U-shaped tube is arranged on the lower layer of the cylinder, and the outer end extends out of the cylinder; the temperature measurement control system, the heating system, the adjustable pump and the ultraviolet sterilizing lamp are all connected in series with the timer and then connected to the main circuit. The utility model can avoid the problems of complicated extraction of particulate matter and lack of pollutants in the extraction process in the evaluation process of atmospheric particulate matter toxicity, resulting in incomplete evaluation.

Description

Atmospheric particle collection device in cell culture medium

technical field

The utility model belongs to the field of environmental toxicology, in particular to a device for collecting atmospheric particles in cell culture medium.

Background technique

Air is the basis for the reproduction of life on earth and the development of human beings. Its state and changes always affect human activities and survival. In 2013, China's smog problem was serious, and large areas of smog broke out in various places. Taking Beijing as an example, there were 26 days of smog in January alone. The emergence of smog has seriously affected human health, increasing the incidence of respiratory infections and other complications. Especially PM2.5 particles, compared with coarser atmospheric particles, have small particle size, large area, strong activity, easy to be accompanied by toxic and harmful substances (such as heavy metals, microorganisms, etc.), and the residence time in the atmosphere Long distance and long transportation distance, so it has a greater impact on human health and atmospheric environment quality.

However, the current research on the toxicity of particulate matter in smog is not in-depth. A large number of studies only use big data to show that the increase of PM2.5 is positively correlated with the number of hospitalized respiratory diseases or the incidence of lung cancer, lacking basic experimental research and theoretical support. The main reason is that the extraction of PM2.5 particles is difficult, including the complexity of the extraction process. At present, the extraction of PM2.5 particles mainly adopts the method of putting the polytetrafluoroethylene filter membrane that collects PM2.5 particles into 2ml of sterile water for water ultrasound, then drying, weighing the quality, and then using sterile water to prepare the PM2.5 The exposure solution was stored at -20°C for use in the cytotoxicity test. This method has the following three defects: 1. The soluble particulate matter in PM2.5 is excluded; 2. There may be condensation during the extraction process of the particulate matter, resulting in a change in particle size; 3. The exposure process is a one-time exposure, which is different from the actual cumulative exposure. Therefore, the current extraction method for PM2.5 has defects, resulting in incomplete toxicity evaluation.

At this stage, some achievements have been made in the collection device and sterilization device for atmospheric fine particles. The patent No. 201010208349.6 and the patent titled "Three-channel Atmospheric Particle Sampler" provide a device for collecting atmospheric particles of different particle sizes; The patent number is 201110408426.7, and the invention name is "Purification treatment device for PM2.5 level dust collection and the killing of bacteria carried by it", which provides a dust sterilization device; but the above two devices cannot be applied to the treatment of atmospheric particulate matter. Cellular toxicity evaluation. For this reason, it is necessary to research and develop a new type of device that integrates atmospheric particulate matter collection, sterilization, and cell exposure to provide new technologies and ideas for the toxicity evaluation of atmospheric particulate matter, which is helpful for reducing the environmental risk of atmospheric fine particulate matter and the impact on the population. Health hazards are significant.

Contents of the invention

The purpose of this utility model is to address the deficiencies of the prior art, to provide a high-efficiency, simple operation, and comprehensive pollutant collection device for collecting atmospheric particulate matter in cell culture medium, to overcome the complexity of atmospheric particulate matter extraction, and the lack of some pollutants during the extraction process , It is difficult to accurately reflect the defects of PM10 and PM2.5 on human tissue cells, making the toxicity evaluation of atmospheric fine particles more accurate.

For solving the problems of the technologies described above, the technical scheme of the utility model is as follows:

The utility model includes a cylinder body, a particulate matter filtration system, a particulate matter degerming system and a cell exposure system; the cylinder body is in the shape of a cylinder with an open top and a closed bottom, and the inner wall is divided into an upper layer, a middle layer and a lower layer by two partitions; The particulate matter filtration system includes a membrane holder and a filter membrane; the bottom of the membrane holder is closed, and is integrally nested in the upper layer of the inner wall of the cylinder; multiple filter membranes are layered on the membrane holder; the particulate matter The sterilization system includes an iron ring, an ultraviolet sterilizing lamp and an air-conducting hose; the bottom of the upper partition and the top of the lower partition are fixed with an ultraviolet sterilizing lamp; It is spiral in the vertical direction and fixed by an iron ring; the upper end of the air guide hose passes through the upper partition and extends into the bottom of the membrane holder, and the lower end passes through the lower partition; the lower end of the air guide hose is provided with flow meter and adjustable pump; the cell exposure system includes a temperature measurement control system, a heating system and a U-shaped tube; the lower side wall of the cylinder is provided with a cell culture dish inlet, and the door plate covers the cell culture dish inlet; the described The U-shaped tube is arranged on the lower layer of the cylinder as a whole, and the outer end extends out of the cylinder; the lower end of the air guide hose and the end of the U-shaped tube extending out of the cylinder are equipped with a one-way valve; the temperature measurement control system and heating The systems are all arranged in the lower layer of the cylinder to form a temperature control system; the temperature measurement control system, heating system, adjustable pump and ultraviolet sterilizing lamp are all connected in series with the timer and then connected to the general circuit.

The cylinder is fixed on an adjustable tripod.

The ultraviolet sterilizing lamp is equipped with a switch, which can adjust the intensity of ultraviolet rays, and can also manually adjust the ultraviolet exposure time.

Both the lower layer of the cylinder body and the inner side wall of the door panel are coated with a heat insulating layer.

The cell exposure system also includes a culture dish placement platform, and the culture dish placement platform is arranged at the bottom of the barrel.

When the gas flows from the top of the air guiding hose to the bottom, the one-way valve on it opens, and when the gas flows from the inner end of the U-shaped tube to the outer end, the one-way valve on it opens.

The beneficial effect that the utility model has:

The utility model integrates atmospheric particulate matter collection and cytotoxicity exposure, and can avoid the complicated extraction of atmospheric particulate matter in the existing evaluation process of atmospheric particulate matter toxicity, and the lack of pollutants in the extraction process, thereby causing the problem of incomplete evaluation, making atmospheric particulate matter pollutants Toxicity assessment is organically combined with local pollutant monitoring data to comprehensively and accurately evaluate the toxicity characteristics of air pollutants in different regions. The utility model has the advantages of simple operation, ingenious design, easy implementation, convenient transportation and accurate toxicity evaluation.

Description of drawings

Fig. 1 is the overall structural representation of the utility model.

Detailed ways

Below in conjunction with accompanying drawing and embodiment the utility model is further described.

As shown in Figure 1, the atmospheric particle collection device in the cell culture medium includes a cylinder body, a particle filter system, a particle sterilization system and a cell exposure system; the cylinder body is fixed on an adjustable tripod 10; the cylinder body is open at the top and closed at the bottom Cylindrical shape with an inner diameter of 25cm, the inner wall is divided into upper, middle and lower layers by two partitions, the upper layer is 15cm high, the middle layer is 25cm high, and the lower layer is 30cm high; the particle filtration system includes membrane fixing frame 1 and filter membrane 2; membrane fixing The bottom of the frame 1 is closed, and is nested in the upper layer of the inner wall of the cylinder as a whole; two filter membranes 2 are placed on the membrane fixing frame 1 in two layers; the particulate matter sterilization system includes an iron ring 3, an ultraviolet sterilizing lamp 4 and an air guide soft Tube 5; two ultraviolet sterilizing lamps 4 are fixed on the bottom of the upper partition, and the other two ultraviolet sterilizing lamps 4 are fixed on the top of the lower partition; the air guide hose 5 is arranged in the middle layer of the cylinder, and the whole is along the vertical direction It is spiral and fixed by the iron ring 3; the upper end of the air guide hose 5 passes through the upper partition and extends into the bottom of the film holder 1, and the lower end passes through the lower partition; the lower end of the air guide hose 5 is provided with Flowmeter 8 and adjustable pump 14 ensure the stability of the airflow; the cell exposure system includes a temperature measurement control system 7, a heating system 9, a petri dish placement platform 11 and a U-shaped tube 15; the lower side wall of the cylinder is provided with cells The entrance of the culture dish, the door plate 12 covers the entrance of the cell culture dish; the lower layer of the cylinder body and the inner wall of the door plate 12 are coated with a heat insulating layer 16; the culture dish placement platform 11 is set at the bottom of the cylinder body; the U-shaped tube 15 is integrally set on the cylinder body The lower layer, and the outer end extends out of the cylinder; the lower end of the air guiding hose 5 and the end of the U-shaped tube 15 extending out of the cylinder are all provided with a one-way valve 6; when the gas flows from the top of the air guiding hose 5 to the bottom When the gas flows from the inner end of the U-shaped pipe 15 to the outer end, the one-way valve 6 on it is opened, and the one-way valve 6 is automatically closed under the condition of no air flow to prevent the external bacteria from entering after the test is stopped; temperature measurement Both the control system 7 and the heating system 9 are arranged in the lower layer of the cylinder to form a temperature control system to ensure that the cells are under normal exposure temperature conditions. The temperature measurement control system 7, the heating system 9, the adjustable pump 14 and all the ultraviolet sterilizing lamps 4 are all connected in series with the timer 13 and then connected to the general circuit. The timer 13 can set the exposure time according to the test requirements.

The height of the membrane holder 1 is 5 cm, and filter membranes 2 with different apertures can be placed therein.

The UV sterilizing lamp 4 is equipped with a switch, which can adjust the intensity of ultraviolet rays, and also can manually adjust the ultraviolet exposure time.

The working principle of the atmospheric particle collection device in the cell culture medium:

First turn on the adjustable pump 14, adjust the air flow into the air guide hose 5 to the vital capacity required for the test, and set the number of breaths per minute, then turn on the temperature measurement control system 7 and the heating system 9 to adjust the temperature of the lower layer of the cylinder to the temperature required for normal cell growth. After the parameters are adjusted, inject sterile water into the U-shaped tube 15 to prevent external bacteria from entering and affect the test results; then put the sterile cell culture dish with cells on the culture dish placement platform 11 through the cell culture dish inlet, The lower end of the air guiding hose 5 and the inner end of the U-shaped pipe 15 are all inserted into the sterile cell culture dish, and the exposure time is set on the timer 13 . During operation, the adjustable pump 14 pumps the outside air to the filter membrane 2, sieves out large particle size particles, and obtains required small particle size particles. After the outside air is filtered by the filter membrane 2, it enters the air guide hose 5 and is irradiated by the ultraviolet sterilizing lamp 4 to sterilize the particulate matter. The sterilized particulate matter enters the sterile cell culture medium, and the excess gas passes through the U-shaped tube 15 out, into the air. After the test, since there is no air flow in the air guide hose 5 and the U-shaped tube 15, the two one-way valves 6 are automatically closed, the sterile cell culture dish is taken out, the biochemical indicators of the cells are detected, and the toxicity of the atmospheric particles is evaluated.

The atmospheric particulate matter collection device in the cell culture medium integrates atmospheric particulate matter collection and cytotoxic exposure, which can avoid the problems of complex extraction of atmospheric particulate matter in the existing atmospheric particulate matter toxicity evaluation process, and the lack of pollutants in the extraction process, resulting in incomplete evaluation. , so that the toxicity evaluation of atmospheric particulate matter pollutants can be organically combined with local pollutant monitoring data, and the toxicity characteristics of air pollutants in different regions can be comprehensively and accurately evaluated.

Claims (6)

1. airborne particulate matter collection device in cell culture medium, comprises cylindrical shell, particulate matter filtering system, particulate matter sterilization system and cell exposure system, it is characterized in that:

Described cylindrical shell is the round shape of open-top, closed bottom, and inwall two pieces of baffle for separatings become the upper, middle and lower; Described particulate matter filtering system comprises film anchor and filter membrane; The closed bottom of described film anchor, entirety is nested in the inwall upper strata of cylindrical shell; Multiple filter membrane layerings are placed on film anchor; Described particulate matter sterilization system comprises iron hoop, ultraviolet sterilization lamp and soft air-guide pipe; The bottom of upper baffle plate and the top of lower partition are all fixed with ultraviolet sterilization lamp; Described soft air-guide pipe is arranged on cylindrical shell middle level, and entirety vertically in the shape of a spiral, and is fixed by iron hoop; The upper end of soft air-guide pipe is passed the dividing plate on top and stretches into bottom film anchor, and lower end is through the dividing plate of bottom; The lower end of soft air-guide pipe is provided with under meter and tunable pump; Described cell exposure system comprises temperature measuring Controlling System, heating system and U-tube; Lower floor's sidewall of cylindrical shell offers Tissue Culture Dish import, and door-plate covers Tissue Culture Dish import; Described U-tube is integrally provided in cylindrical shell lower floor, and outer end is stretched out outside cylindrical shell; One end that the lower end of soft air-guide pipe and U-tube are stretched out outside cylindrical shell is equipped with check valve; Described temperature measuring Controlling System and heating system are all arranged on cylindrical shell lower floor, composition temperature controlling system; Described temperature measuring Controlling System, heating system, tunable pump and ultraviolet sterilization lamp access way circuit after all connecting with timer.

2. airborne particulate matter collection device in cell culture medium according to claim 1, is characterized in that: described cylindrical shell is fixed on adjustable tripod.

3. airborne particulate matter collection device in cell culture medium according to claim 1, is characterized in that: described ultraviolet sterilization lamp is furnished with switch, adjustable uitraviolet intensity, also can the manual regulation UV exposure time.

4. airborne particulate matter collection device in cell culture medium according to claim 1, is characterized in that: described cylindrical shell lower floor and the inner side-wall of door-plate all scribble thermofin.

5. airborne particulate matter collection device in cell culture medium according to claim 1, is characterized in that: described cell exposure system also comprises culture dish placement platform, and described culture dish placement platform is arranged on cylinder body bottom.

6. airborne particulate matter collection device in cell culture medium according to claim 1, is characterized in that: the one-way valve opens direction on described soft air-guide pipe be top to bottom direction, the one-way valve opens direction in U-tube is inner to outer extreme direction.