CN106047543A - Abelmoschus manihot health-care wine for preventing and treating diabetes and preparation method thereof - Google Patents

Abelmoschus manihot health-care wine for preventing and treating diabetes and preparation method thereof Download PDF

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Publication number
CN106047543A
CN106047543A CN201610481260.4A CN201610481260A CN106047543A CN 106047543 A CN106047543 A CN 106047543A CN 201610481260 A CN201610481260 A CN 201610481260A CN 106047543 A CN106047543 A CN 106047543A
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parts
wine
abelmoschus manihot
health care
preparation
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Inventor
王飞
王一飞
王闯
马婧
王巧利
任哲
王林涛
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Guangzhou Jinan Biomedicine Research and Development Base Co Ltd
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Guangzhou Jinan Biomedicine Research and Development Base Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G1/00—Preparation of wine or sparkling wine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13—Coniferophyta (gymnosperms)
    • A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/27—Asclepiadaceae (Milkweed family), e.g. hoya
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/41—Crassulaceae (Stonecrop family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/42—Cucurbitaceae (Cucumber family)
    • A61K36/424—Gynostemma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/62—Nymphaeaceae (Water-lily family)

Abstract

The invention belongs to the field of health-care products, and particularly relates to an Abelmoschus manihot health-care wine for preventing and treating diabetes and a preparation method thereof. The Abelmoschus manihot health-care wine is prepared from the following raw materials in parts by weight: 18-30 parts of Abelmoschus manihot, 10-20 parts of kudzu root, 6-15 parts of gynostemma pentaphyllum, 5-10 parts of swallowwort rhizome, 10-15 parts of lotus leaf, 5-15 parts of Rhodiola rosea, 10-18 parts of Persea americana Mill peel, 8-20 parts of pine needles, 3-10 parts of Suaeda sulsa, 12-20 parts of Semen Euryales, 3-7 parts of L-arabinose and 50-75 parts of dry red wine. The Abelmoschus manihot health-care wine provided by the invention has the advantages of low sugar content and low alcohol content, and is suitable for patients with diabetes to drink. The Abelmoschus manihot health-care wine provided by the invention has abundant health-care nutrient components, has the effects of nourishing yin, promoting blood circulation, reinforcing the spleen, tonifying the kidney, invigorating qi, promoting salivation, promoting the circulation of qi and relieving the depressed liver, is capable of regulating the blood sugar and blood fat metabolism of the human body and assisting in lowering the blood sugar and enhancing the immunity of the human body, and has obvious prevention and improvement effects on diabetes.

Description

A kind of Abelmoschus manihot health care wine preventing and treating diabetes and preparation method thereof
Technical field
The invention belongs to health product technology field, be specifically related to a kind of Abelmoschus manihot health care wine preventing and treating diabetes and preparation thereof Method.
Background technology
Hibiscus manihot L. (formal name used at school: Hibiseu Smanihot L.), has another name called vegetable Furong, the wildest Hibisci Mutabilis, for annual herb brocade Kurrajong Abelmoschus plant, some areas another name is glutinous dry or wych-elm skin.Hibiscus manihot L. is multiple rich in biological flavone and needed by human body Aminoacid and trace element, measure Hibiscus manihot L. flavones content and show, Hibiscus manihot L. flavones content is up to 5.6% (dry weight), is Know content soprano in natural product.Flower of Hibiscus manihot L dietotherapeutic, containing various bioactivators, wherein, modern medicine grinds Studying carefully and show, the total flavones of flower of Hibiscus manihot L has the effects such as analgesia, anti-cerebral ischemia and treatment oral ulcer.And full price albumen contained by it Matter, high polysaccharide glue, dietary fiber, trace element-selenium, zinc, multiple non-saponifiable matter etc., have and significantly regulate human endocrine, immunity Power, enhancing body oxidation resistance, antiinflammatory, town pain, the effect such as resisting fatigue, defying age, anticancer, anti-cancer, blood fat reducing.
Chinese patent application 2015101925358 discloses a kind of earthworm healthcare wine and processing technology, described health promoting wine Each component consists of earthworm extract 10～30 parts, 65～60 degree of Chinese liquor 65～85 parts and Hibiscus manihot L. 10～25 by mass fraction Part.The earthworm healthcare wine that this patent provides has blood pressure lowering dredging collateral, improves microcirculation in human body, prevents cardiovascular and cerebrovascular disease, strengthens machine The effects such as body immunity, but it is inconspicuous for the reducing effect of blood glucose, and owing to its alcohol content is high, it is unsuitable for diabetes patient Take.
Diabetes be one group because insulin is absolute or insulin sensitivity is dropped by relative hyposecretion and target tissue cell Low cause a series of metabolism disorder syndromes such as internal sugar, protein, fat and electrolyte, with glucose tolerance reduction, blood glucose Increase and be characterized with glycosuria.In early days the most asymptomatic clinically, being developed to the symptom phase just has polyphagia, polydipsia, polyuria, excessive thirst, polyphagia, disappears The syndrome such as thin, tired.Patients on long-term, often occur together the pathological changes such as cardiovascular, kidney, eye and nerve.The model that diabetes spp is quenched one's thirst in the traditional Chinese medical science Farmland, its Etiological can be divided into following 5 points in the traditional Chinese medical science: 1) innate deficiency, the five internal organs are weak；2) feelings will is uncomfortable, strongly fragrant long fire-transformation； 3) eating and drinking without temperance, intrinsic heat impairment of body fluid；4) diseases caused by exogenous pathogenic factor six climate exopathogens, heat-transformation impairment of YIN；5) forced labour mistake degree, impairment of the kidney due to indulgence in sexual activities.The sugar of literature research at present The pathogenesis of urine disease mainly has hyperactivity of fire caused by deficiency of YIN, deficiency of both QI and YIN, deficiency in both YIN and YANG, obstruction of collaterals by blood stasis, insufficiency of the spleen leading to overabundance of dampness etc..Controlling of current diabetes Treat and mainly supplement to come by the way of combining by diet control cooperation hypoglycemic medicine (for type ii diabetes) or insulin Treatment diabetes.
Chinese patent CN 101607031 B disclose a kind of medicated wine treating and preventing diabetes and preparation method thereof and Using method, this medicated wine is prepared by the raw materials in: the Cortex Eucommiae 10～30, Radix Dipsaci 10～30, Radix Asparagi 10～30, Semen euryales 20 ～40, Semen Cuscutae 40～60, Fructus Corni 40～60, Radix Rehmanniae Preparata 10～30, the Radix Rehmanniae 5～20, RHIIZOMA DIOSCOREAE fr0m Henan of China 5～20, Semen Nelumbinis 5～20, bar Halberd sky 5～20, Radix Polygoni Multiflori 5～20, Radix Notoginseng 5～20, the Radix Astragali 20～40, Radix Salviae Miltiorrhizae 15～30, Radix Ginseng 5～15, Cordyceps 5～15 He Radix Glycyrrhizae 5～15, its preparation method, for be cleaned by above-mentioned raw materials medical material, is processed into coarser powder and puts into cloth bag, steeps 6 with high spirit submergence ～15 days, taking out cloth bag, then the cloth bag of taking-up is put into submergence bubble 10～20 days in yellow wine, obtain described medicated wine, this medicated wine is treated Effect is notable, taking convenience.
Chinese patent CN 101125177 B discloses a kind of Miao's wine treating diabetes, three kinds of medicated wine mix, First medicated wine is by 15 taste herbal medicine immersions such as Lignum Santali Albi, Lignum Aquilariae Resinatum, Herba Cistanches in the alcoholic strength height pure grain wine not less than 50 degree, Sealing is deposited and is stored half a year；The second medicated wine is at wine by 12 taste herbal medicine immersions such as RHIIZOMA DIOSCOREAE fr0m Henan of China, propolis, Ganoderma, Radix Panacis Quinquefolii, Radix Pseudostellariaes In the rice vinegar wine of precision about 12 degree, seal cellar for storing things and deposit half a year；The third medicated wine is by Radix Platycodonis, RADIX PUERARIAE, Carapax Et Plastrum Testudinis (processed), processs Trionyx sinensis Wiegmann First-class 11 taste herbal medicine immersions, in alcoholic strength is about the low-alcohol rice wine of about ten degree, seals cellar for storing things and deposit half a year, finally by above-mentioned leaching The medicated wine soaked uniformly is mixed by the weight ratio of 5:5:8 successively, through steps Temperature Treatment and get final product.Described Miao's wine has coordination The dirty function of liver,spleen,kidney three, and the function such as reducing blood glucose, balancing blood fat, regulation blood pressure, can be used for treating diabetes.
Existing preventing and treating diabetic health care wine is many to be mixed immersion and prepares by raw material with wine, and material effective component leaches slow, leaching Going out rate low, raw material availability is low, and alimentary health-care function is not enough；And use some expensive Chinese medicines, such as Radix Ginseng, Radix Notoginseng etc., raw material more Cost is high, and the health promoting wine prepared also more exists the problems such as flavour of a drug are dense, mouthfeel is puckery, bitter, poor stability, thus limits health care The promotion and application of wine.Meanwhile, existing preventing and treating diabetic health care wine many uses Chinese liquor and yellow wine are as base liquor, sugar content and ethanol Spending higher, diabetes patient is drinking frequency and the amount of drinking aspect has the restriction that comparison is strict, thus further limit this The development of class health promoting wine.
Summary of the invention
It is an object of the invention to overcome existing technological deficiency, it is provided that a kind of Abelmoschus manihot health care wine preventing and treating diabetes and Its preparation method.The Abelmoschus manihot health care wine formula that the present invention provides is reasonable, and cost of material is low, the Abelmoschus manihot health care wine prepared Nutritive and health protection components enriches, and has nourishing yin and promoting blood circulation, effect of reinforcing spleen and kidney, supplementing QI for promoting the production of body fluid and promoting QI circulation for relieving depression, can regulate human body blood Diabetes are had and significantly prevent and improve effect by sugar and blood lipid metabolism, auxiliary hyperglycemic and enhancing body immunity.Meanwhile, The Abelmoschus manihot health care wine wine body clarification that the present invention provides is bright, and mouthfeel is coordinated easypro suitable, and delicate fragrance is light refreshing, and without taste of Chinese medicine, stability is strong.
Technical scheme is as follows:
A kind of Abelmoschus manihot health care wine preventing and treating diabetes, by weight, described Abelmoschus manihot health care wine prepare raw material bag Include following composition: Hibiscus manihot L. 18～30 parts, Radix Puerariae 10～20 parts, Herb Gynostemmae Pentaphylli 6～15 parts, Rhizoma Cynanchi Stauntonii 5～10 parts, Folium Nelumbinis 10～15 Part, Radix Rhodiolae 5～15 parts, Camphor tree Pericarpium Pyri 10～18 parts, Folium Pini 8～20 parts, Suaeda salsa 3～10 parts, Semen Euryales 12～20 parts, L-I Uncle's sugar 3～7 parts and claret 50～75 parts.
Preferably, by weight, the raw material of preparing of described Abelmoschus manihot health care wine includes following composition: Hibiscus manihot L. 28 parts, Radix Puerariae 15 parts, Herb Gynostemmae Pentaphylli 10 parts, Rhizoma Cynanchi Stauntonii 7 parts, 10 parts of Folium Nelumbinis, Radix Rhodiolae 8 parts, Camphor tree Pericarpium Pyri 18 parts, Folium Pini 15 parts, Suaeda salsa 5 parts, Semen Euryales 18 parts, L-arabinose 5 parts and claret 65 parts.
The preferred flower of Hibiscus manihot L of Hibiscus manihot L. of the present invention.
The present invention provide the Abelmoschus manihot health care wine preventing and treating diabetes with claret as base liquor, rich in tannin, pigment, The polyphenol substances such as flavonoid, have the functions such as antioxidation, blood circulation promoting, aid digestion, diuresis, can prevent and treat cardiovascular and cerebrovascular disease Generation with prevention diabetic complication.The Abelmoschus manihot health care wine formula that the present invention provides is reasonable, and cost of material is low, extra dry red wine Fructus Vitis viniferae The common Chinese medicine materials such as wine and Hibiscus manihot L., Radix Puerariae, Herb Gynostemmae Pentaphylli compound the Abelmoschus manihot health care wine prepared and have nourishing yin and promoting blood circulation, spleen reinforcing Effect of kidney tonifying, supplementing QI for promoting the production of body fluid and promoting QI circulation for relieving depression, can regulate blood sugar for human body and blood lipid metabolism, auxiliary hyperglycemic and enhancing human body and exempt from Epidemic disease power, has diabetes particularly type ii diabetes and significantly prevents and improve effect.Meanwhile, with claret for base liquor system The Abelmoschus manihot health care wine sugar content and the alcoholic strength that obtain are low, are suitable to patients with diabetes mellitus.
Correspondingly, the present invention also provides for the preparation side of a kind of Abelmoschus manihot health care wine preventing and treating diabetes of the present invention Method, comprises the steps:
S1: depletion tree mallow, Herb Gynostemmae Pentaphylli, Rhizoma Cynanchi Stauntonii, Camphor tree Pericarpium Pyri and Semen Euryales, roguing dry, pulverize after cleaning, and crosses 40～60 mesh Sieve, adds water and the cellulase of 0.5～1.0 ‰ of medical material gross mass 6～10 times amount, is 40～60 DEG C in temperature, and pH is 4.2 ～enzymolysis 1～2h under conditions of 4.7, the most ultrasonic extraction 45～60min, it is eventually adding the sugar of medical material gross mass 0.2～0.5 ‰ Changing enzyme, be 60～65 DEG C in temperature, pH is enzymolysis 1～2h under conditions of 4.0～4.5, filters, and filtrate reduced in volume is to original volume 1/8～1/10, obtain extracting solution I；
S2: take Radix Puerariae, Folium Nelumbinis, Radix Rhodiolae, Folium Pini and Suaeda salsa, roguing dry, pulverize after cleaning, and crosses 40～60 mesh sieves, Add the ethanol of medical material gross mass 8～12 times amount, ultrasonic extraction 1～2 times, each 1～2h, filter, merging filtrate, filtrate decompression Ethanol is distilled off, is evaporated to the 1/8～1/10 of original volume, obtains extracting solution II；
S3: take the Angel wine high activity dried yeast of claret quality 0.05～0.1 ‰, by activating solution after activation Merge with extracting solution II described in extracting solution I and S2 described in claret, S1, at the bar that temperature is 15～20 DEG C after stirring Sealed fermenting 10～20 days under part, filter, obtain Abelmoschus manihot health care wine stock solution；
S4: added by L-arabinose in Abelmoschus manihot health care wine stock solution described in S3, adds purified water regulation wine after stirring and dissolving Precision is 8～12 °, add clear stable agent, after stirring and evenly mixing temperature be-8～-4 DEG C under conditions of standing 10～15 days；
S5: filter, sterilizing, bottling, obtain Abelmoschus manihot health care wine of the present invention.
Preferably, in described step S1, the temperature of ultrasonic extraction is 70～85 DEG C, and power is 450～800W.
Preferably, in described step S2, the volume fraction of ethanol is 60～80%.
Preferably, in described step S2, the temperature of ultrasonic extraction is 60～75 DEG C, and power is 300～500W.
Preferably, in described step S3, the activation method of Angel wine high activity dried yeast is that Angel wine is high Active dry yeast by liquid-solid ratio 18～22mL/g add 38～40 DEG C containing crystal sugar 4～8% warm water in stir 25～40min, Obtain activating solution.
Preferably, in described step S4, clarifier is selected from bentonite, chitosan, gelatin, casein and kieselguhr Plant or multiple.
Preferably, the addition of described clarifier is 0.2～0.4g/L.
Preferably, described clarifier is chitosan and bentonitic mixture, counts in mass ratio, chitosan: bentonite is 0.4～0.7:1.
Compared with prior art, the beneficial effects of the present invention is:
1) the Abelmoschus manihot health care wine preventing and treating diabetes that the present invention provides is with claret as base liquor, sugar content and ethanol Spend low, be suitable to patients with diabetes mellitus；
2) the Abelmoschus manihot health care wine formula that the present invention provides is reasonable, and cost of material is low, the Abelmoschus manihot health care wine prepared Nutritive and health protection components enriches, and has nourishing yin and promoting blood circulation, effect of reinforcing spleen and kidney, supplementing QI for promoting the production of body fluid and promoting QI circulation for relieving depression, can regulate human body blood Diabetes are had and significantly prevent and improve effect by sugar and blood lipid metabolism, auxiliary hyperglycemic and enhancing body immunity；
3) the Abelmoschus manihot health care wine wine body clarification that the present invention provides is bright, and mouthfeel is coordinated easypro suitable, and delicate fragrance is light refreshing, without Chinese medicine Taste, stability is strong.
Detailed description of the invention
Further describe the present invention below by way of detailed description of the invention, but the present invention is not limited only to following example.At this In the range of invention or without departing from present disclosure, spirit and scope, the present invention is suitably modified, replaces merit Imitate identical component, will become apparent to those skilled in the art that they are considered as being included in the model of the present invention Within enclosing.
Embodiment 1 present invention prevents and treats the preparation of the Abelmoschus manihot health care wine of diabetes
The embodiment of the present invention 1 prevents and treats the Abelmoschus manihot health care wine of diabetes, and by weight, it is prepared raw material and includes becoming as follows Point: Hibiscus manihot L. 18 parts, Radix Puerariae 15 parts, Herb Gynostemmae Pentaphylli 10 parts, Rhizoma Cynanchi Stauntonii 5 parts, 10 parts of Folium Nelumbinis, Radix Rhodiolae 8 parts, Camphor tree Pericarpium Pyri 10 parts, Folium Pini 15 parts, Suaeda salsa 5 parts, Semen Euryales 15 parts, L-arabinose 7 parts and claret 75 parts.
Preparation method:
S1: depletion tree mallow, Herb Gynostemmae Pentaphylli, Rhizoma Cynanchi Stauntonii, Camphor tree Pericarpium Pyri and Semen Euryales, roguing dry, pulverize after cleaning, and crosses 40 mesh sieves, adds Entering water and the cellulase of 0.6 ‰ of medical material gross mass 6 times amount, be 50 DEG C in temperature, pH is enzymolysis 1h under conditions of 4.5, so After be 80 DEG C in temperature, power is ultrasonic extraction 50min under conditions of 700W, is eventually adding the saccharifying of medical material gross mass 0.3 ‰ Enzyme, is 60 DEG C in temperature, and pH is enzymolysis 2h under conditions of 4.5, filters, the 1/10 of filtrate reduced in volume to original volume, obtains extraction Liquid I；
S2: take Radix Puerariae, Folium Nelumbinis, Radix Rhodiolae, Folium Pini and Suaeda salsa, roguing dry, pulverize after cleaning, and crosses 40 mesh sieves, adds The ethanol that volume fraction is 75% of medical material gross mass 12 times amount, is 60 DEG C in temperature, and power is ultrasonic leaching under conditions of 400W Carrying 2 times, each 1.5h, filter, merging filtrate, filtrate decompression is distilled off ethanol, is evaporated to the 1/10 of original volume, must carry Take liquid II；
S3: take the Angel wine high activity dried yeast of claret quality 0.05 ‰, is added by solid-to-liquid ratio 20mL/g 40 DEG C of warm waters containing crystal sugar 4% stir 30min, obtains activating solution, then activating solution is extracted with described in claret, S1 Extracting solution II described in liquid I and S2 merges, and after stirring, sealed fermenting 20 days, filtration under conditions of temperature is 18 DEG C, obtains golden Tree mallow health promoting wine stock solution；
S4: added by L-arabinose in Abelmoschus manihot health care wine stock solution described in S3, adds purified water regulation wine after stirring and dissolving Precision is 12 °, adds the bentonite of 0.4g/L, stands 15 days after stirring and evenly mixing under conditions of temperature is-4 DEG C；
S5: filter, sterilizing, bottling, obtain Abelmoschus manihot health care wine described in the embodiment of the present invention 1.
Embodiment 2 present invention prevents and treats the preparation of the Abelmoschus manihot health care wine of diabetes
The embodiment of the present invention 2 prevents and treats the Abelmoschus manihot health care wine of diabetes, and by weight, it is prepared raw material and includes becoming as follows Point: Hibiscus manihot L. 28 parts, Radix Puerariae 15 parts, Herb Gynostemmae Pentaphylli 10 parts, Rhizoma Cynanchi Stauntonii 7 parts, 10 parts of Folium Nelumbinis, Radix Rhodiolae 8 parts, Camphor tree Pericarpium Pyri 18 parts, Folium Pini 15 parts, Suaeda salsa 5 parts, Semen Euryales 18 parts, L-arabinose 5 parts and claret 65 parts.
Preparation method:
S1: depletion tree mallow, Herb Gynostemmae Pentaphylli, Rhizoma Cynanchi Stauntonii, Camphor tree Pericarpium Pyri and Semen Euryales, roguing dry, pulverize after cleaning, and crosses 60 mesh sieves, adds Entering water and the cellulase of 0.8 ‰ of medical material gross mass 10 times amount, be 50 DEG C in temperature, pH is enzymolysis 2h under conditions of 4.5, so After be 85 DEG C in temperature, power is ultrasonic extraction 50min under conditions of 600W, is eventually adding the saccharifying of medical material gross mass 0.5 ‰ Enzyme, is 65 DEG C in temperature, and pH is enzymolysis 2h under conditions of 4.3, filters, the 1/10 of filtrate reduced in volume to original volume, obtains extraction Liquid I；
S2: take Radix Puerariae, Folium Nelumbinis, Radix Rhodiolae, Folium Pini and Suaeda salsa, roguing dry, pulverize after cleaning, and crosses 60 mesh sieves, adds The ethanol that volume fraction is 70% of medical material gross mass 10 times amount, is 60 DEG C in temperature, and power is ultrasonic leaching under conditions of 400W Carrying 2 times, each 2h, filter, merging filtrate, filtrate decompression is distilled off ethanol, is evaporated to the 1/10 of original volume, obtains extraction Liquid II；
S3: take the Angel wine high activity dried yeast of claret quality 0.08 ‰, is added by solid-to-liquid ratio 20mL/g 40 DEG C of warm waters containing crystal sugar 5% stir 30min, obtains activating solution, then activating solution is extracted with described in claret, S1 Extracting solution II described in liquid I and S2 merges, and after stirring, sealed fermenting 20 days, filtration under conditions of temperature is 18 DEG C, obtains golden Tree mallow health promoting wine stock solution；
S4: added by L-arabinose in Abelmoschus manihot health care wine stock solution described in S3, adds purified water regulation wine after stirring and dissolving Precision is 10 °, adds the clear stable agent of 0.3g/L, stands 15 days after stirring and evenly mixing under conditions of temperature is-4 DEG C；Wherein, Described clear stable agent is chitosan and the mixture of bentonite 0.6:1 in mass ratio mixing；
S5: filter, sterilizing, bottling, obtain Abelmoschus manihot health care wine described in the embodiment of the present invention 2.
Embodiment 3 present invention prevents and treats the preparation of the Abelmoschus manihot health care wine of diabetes
The embodiment of the present invention 3 prevents and treats the Abelmoschus manihot health care wine of diabetes, and by weight, it is prepared raw material and includes becoming as follows Point: Hibiscus manihot L. 30 parts, Radix Puerariae 20 parts, Herb Gynostemmae Pentaphylli 6 parts, Rhizoma Cynanchi Stauntonii 10 parts, 10 parts of Folium Nelumbinis, Radix Rhodiolae 5 parts, Camphor tree Pericarpium Pyri 10 parts, Folium Pini 8 Part, Suaeda salsa 10 parts, Semen Euryales 12 parts, L-arabinose 6 parts and claret 70 parts.
Preparation method is with embodiment 2.
Comparative example 1
Comparative example 1 Abelmoschus manihot health care wine, by weight, it is prepared raw material and includes following composition: Hibiscus manihot L. 28 parts, Radix Puerariae 15 parts, Herb Gynostemmae Pentaphylli 10 parts, Rhizoma Cynanchi Stauntonii 7 parts, 10 parts of Folium Nelumbinis, Stigma Maydis 8 parts, Camphor tree Pericarpium Pyri 18 parts, Folium Pini 15 parts, Suaeda salsa 5 parts, Semen Euryales 18 parts, L-arabinose 5 parts and claret 65 parts.
Preparation method is similar to embodiment 2.
Difference with embodiment 2 is, Radix Rhodiolae is replaced with Stigma Maydis.
Comparative example 2
Comparative example 2 Abelmoschus manihot health care wine, by weight, it is prepared raw material and includes following composition: Hibiscus manihot L. 28 parts, Radix Puerariae 15 parts, Herb Gynostemmae Pentaphylli 18 parts, Rhizoma Cynanchi Stauntonii 7 parts, 10 parts of Folium Nelumbinis, Radix Rhodiolae 18 parts, Folium Pini 15 parts, Suaeda salsa 5 parts, Semen Euryales 18 parts, L-Ah Draw uncle's sugar 5 parts and claret 65 parts.
Preparation method is similar to embodiment 2.
Difference with embodiment 2 is, without Camphor tree Pericarpium Pyri, increases Herb Gynostemmae Pentaphylli and the consumption of Radix Rhodiolae.
Test example one
1, given the test agent: the Abelmoschus manihot health care wine of embodiment 1～3, comparative example 1～2 preparation, claret (use purification It is 10 ° that water adjusts alcoholic strength).
2, experimental animal selects: healthy adult Wistar rat, male, (150 ± 20) g, SPF level, cures purchased from Guangdong Province Learn Experimental Animal Center.
3, the Abelmoschus manihot health care wine of the present invention impact on normal rat blood sugar value
3.1 test methods:
Taking 48 rats, test is omnidistance commonly to maintain forage feed, and after buying, under barrier environment, adaptability feeds 1 In week, fasting takes tail vein blood glucose meter and measures fasting blood sugar (FBG) after can't help water 12h, according to blood sugar level by all greatly Mus is randomly divided into 6 groups, often group 8.It is respectively Normal group, positive controls, embodiment 1～3 groups and comparative example 1～2 groups, Wherein, Normal group gavage gives equal-volume normal saline, and positive controls gavage gives claret, embodiment 1～3 Group, comparative example 1～the 2 groups corresponding Hibiscus manihot L. that gavage gives the embodiment of the present invention 1～3, comparative example 1～2 prepares respectively is protected Strong wine, given low is 25mL/kg, the most each gavage once, continuous gavage 30 days.
After last gavage gives corresponding given the test agent, all Rat Fast can't help water 12h, and tail venous blood sampling measures fasting blood Sugar value.3.2 result of the tests: be shown in Table 1.
The impact on normal rats blood glucose value of the table 1 Abelmoschus manihot health care of the present invention wine
Group N Blood glucose value (mmol L before test-1) Blood glucose value (mmol L after test-1)
Normal group 8 5.46±0.42 5.65±0.58Δ*
Positive controls 8 5.36±0.78 5.42±0.36Δ*
Embodiment 1 group 8 5.58±0.62 5.72±0.75Δ*
Embodiment 2 groups 8 5.64±0.58 5.86±0.42Δ*
Embodiment 3 groups 8 5.50±0.54 5.86±0.48Δ*
Comparative example 1 group 8 5.63±0.65 5.76±0.74Δ*
Comparative example 2 groups 8 5.59±0.73 5.48±0.97Δ*
Note: after test, blood glucose value compares with blood glucose value before test,ΔP ＞ 0.05；After test in blood glucose value, with normal control Group compares,*P ＞ 0.05.
From table 1 result of the test, before and after test, the blood glucose value of each group rat is without notable change (P ＞ 0.05), test After, the blood glucose value of blood glucose value and rats in normal control group that gavage gives the rats in test groups of corresponding given the test agent compares, without aobvious Write difference (P ＞ 0.05), illustrate that the blood glucose value of normal rat is not made significant difference by the Abelmoschus manihot health care wine that the present invention prepares.
4, the Abelmoschus manihot health care wine of the present invention impact on type ii diabetes rat model
4.1 modeling
Taking 75 rats, maintain feedstuff adaptability to feed 1 week with common under barrier environment, fasting takes after can't help water 12h Tail vein blood glucose meter measures fasting blood sugar, randomly selects 8 rats as Normal group, modeling according to blood sugar level Test is omnidistance commonly to maintain forage feed；Residue rat is modeling group, and modeling test is omnidistance feeds with high lipid food, wherein, The formula of high lipid food is: Adeps Sus domestica 10%, sucrose 20%, yolk powder 10%, common maintenance feedstuff 60%.
After high lipid food feeds 4 weeks, modeling group Rat Fast can't help water 12h, lumbar injection streptozotocin STZ After 35mg/kg, 72h, all modeling rat tail veins take hematometry fasting blood sugar, with FBG >=16.7mmol L-1For becoming mould mark After standard, and 1 week, blood glucose is considered as modeling success without notable change person.This test obtains type ii diabetes rat model 59 altogether.
4.2 test method
Taking 8 rats and 56 modeling successful type ii diabetes rat models of modeling group of Normal group, fasting is not After prohibiting water 12h, tail venous blood sampling measures fasting blood sugar, and wherein 56 type ii diabetes rat models are divided at random by blood sugar level Become 7 groups, often group 8, respectively model control group, positive controls, embodiment 1～3 groups and comparative example 1～2 groups.Wherein, just Often matched group and model control group gavage give equal-volume normal saline, and positive controls gavage gives claret, implement Example 1～3 groups, comparative example 1～the 2 groups corresponding gold that gavage gives the embodiment of the present invention 1～3, comparative example 1～2 prepares respectively Tree mallow health promoting wine, given low is 25mL/kg, the most each gavage once, continuous gavage 30 days.Rats in normal control group test is complete Journey is commonly to maintain forage feed；The test of type ii diabetes rat model is omnidistance feeds with high lipid food.
After last gavage gives corresponding given the test agent, water 12h is can't help in fasting, and each group rat tail vein takes hematometry fasting blood Sugar value, respectively group rat blood sugar value and blood glucose decline percentage rate.Computing formula: blood glucose declines percentage rate=[(blood glucose before test Blood glucose value after value-test) the front blood glucose of/test] × 100%.
Respectively group rats by intraperitoneal injection 3mL/kg 10% chloral hydrate solution is anaesthetized, ventral aorta blood sampling detection T-CHOL (TC) and the content of triglyceride (TG) in each group rat blood serum.Finally putting to death rat, observing each internal organs has nontoxic Property change.
4.3 result of the tests: be shown in Table 2.
The impact on type ii diabetes rat model of the table 2 Abelmoschus manihot health care of the present invention wine
Note: after test, blood glucose value compares with blood glucose value before test,ΔP ＜ 0.01；Compare with Normal group,*P ＜ 0.01； Compare with model control group,#P ＜ 0.05,##P ＜ 0.01.
This test example 1～3 groups of continuous gavages of type ii diabetes rat model give the embodiment of the present invention 1～3 system The standby Abelmoschus manihot health care wine obtained 30 days, none is dead for process of the test rat, puts to death rat and observe each internal organs not after off-test Find toxic reaction, illustrate that Abelmoschus manihot health care wine safety of the present invention is high.
As seen from the results in Table 2, before and after test, gavage gives the Abelmoschus manihot health care wine that the embodiment of the present invention 1～3 prepares Embodiment 1～the blood glucose value of 3 groups of type ii diabetes rat models be all remarkably decreased (P ＜ 0.01), Hibiscus manihot L. of the present invention is described Health promoting wine can reduce the blood glucose value of type ii diabetes rat model, though and the blood glucose of comparative example 1～2 groups of rats has declined, But DeGrain.Meanwhile, TC, TG value of embodiment 1～3 groups of type ii diabetes rat models and model control group phase after test Ratio, is remarkably decreased (P ＜ 0.01), illustrates that Abelmoschus manihot health care wine of the present invention raises tool to TC, TG of type ii diabetes rat model There is obvious regulation effect.
In sum, the blood glucose value of normal rat is not made significant difference by the Abelmoschus manihot health care wine that the present invention provides, simultaneously can Significantly reduce blood glucose value and TC, TG value of type ii diabetes rat model, illustrate that Abelmoschus manihot health care wine of the present invention is reducing blood glucose On the basis of, TC, TG value is also had significant regulation effect, can be used for preventing and treating diabetes, particularly type ii diabetes, its In, the effect of embodiment 2 is the most notable, for preferred embodiment.
Test example two
1, test material: embodiment 1-3 prepares Abelmoschus manihot health care wine.
2, test method:
With reference to the test method of " Chinese Pharmacopoeia two annex XIX C of version in 2010 ", Hibiscus manihot L. is prepared by embodiment 1-3 and protects Strong wine carries out long term test, observes Hibiscus manihot L. Abelmoschus manihot health care colours of wine and color and luster.
3, result of the test:
Result of the test is as shown in table 3.
The long-term test results of table 3 Abelmoschus manihot health care wine
As shown in Table 2, the Abelmoschus manihot health care wine that prepared by embodiment of the present invention 1-3 is 25 DEG C ± 2 DEG C, relative humidity in temperature Be place 18 months under conditions of 60% ± 10% after still clarification, glossy, Abelmoschus manihot health care wine prepared by the present invention is described There is higher stability, can effectively improve the organoleptic indicator of Abelmoschus manihot health care wine, be conducive to long-term preservation.
Above content is to combine concrete preferred implementation further description made for the present invention, it is impossible to assert Being embodied as of the present invention is confined to these explanations.For general technical staff of the technical field of the invention, On the premise of present inventive concept, it is also possible to make some simple deduction or replace, all should be considered as belonging to the present invention's Protection domain.

Claims (10)

1. the Abelmoschus manihot health care wine preventing and treating diabetes, it is characterised in that by weight, the system of described Abelmoschus manihot health care wine Standby raw material includes following composition: Hibiscus manihot L. 18～30 parts, Radix Puerariae 10～20 parts, Herb Gynostemmae Pentaphylli 6～15 parts, Rhizoma Cynanchi Stauntonii 5～10 parts, Folium Nelumbinis 10～15 parts, Radix Rhodiolae 5～15 parts, Camphor tree Pericarpium Pyri 10～18 parts, Folium Pini 8～20 parts, Suaeda salsa 3～10 parts, Semen Euryales 12～20 parts, L-arabinose 3～7 parts and claret 50～75 parts.
Prevent and treat the Abelmoschus manihot health care wine of diabetes the most as claimed in claim 1, it is characterised in that by weight, described gold The raw material of preparing of tree mallow health promoting wine includes following composition: Hibiscus manihot L. 28 parts, Radix Puerariae 15 parts, Herb Gynostemmae Pentaphylli 10 parts, Rhizoma Cynanchi Stauntonii 7 parts, Folium Nelumbinis 10 parts, Radix Rhodiolae 8 parts, Camphor tree Pericarpium Pyri 18 parts, Folium Pini 15 parts, Suaeda salsa 5 parts, Semen Euryales 18 parts, L-arabinose 5 parts and extra dry red wine Fructus Vitis viniferae Wine 65 parts.
3. the preparation method of the Abelmoschus manihot health care wine preventing and treating diabetes as claimed in claim 1, it is characterised in that described Preparation method comprises the steps:
S1: depletion tree mallow, Herb Gynostemmae Pentaphylli, Rhizoma Cynanchi Stauntonii, Camphor tree Pericarpium Pyri and Semen Euryales, roguing dry, pulverize after cleaning, and crosses 40～60 mesh sieves, adds Entering water and the cellulase of 0.5～1.0 ‰ of medical material gross mass 6～10 times amount, be 40～60 DEG C in temperature, pH is 4.2～4.7 Under conditions of enzymolysis 1～2h, the most ultrasonic extraction 45～60min, be eventually adding the saccharifying enzyme of medical material gross mass 0.2～0.5 ‰, Being 60～65 DEG C in temperature, pH is enzymolysis 1～2h under conditions of 4.0～4.5, filters, the 1/8 of filtrate reduced in volume to original volume ～1/10, obtain extracting solution I；
S2: take Radix Puerariae, Folium Nelumbinis, Radix Rhodiolae, Folium Pini and Suaeda salsa, roguing dry, pulverize after cleaning, and crosses 40～60 mesh sieves, adds The ethanol of medical material gross mass 8～12 times amount, ultrasonic extraction 1～2 times, each 1～2h, filter, merging filtrate, filtrate decompression is distilled Remove ethanol, be evaporated to the 1/8～1/10 of original volume, obtain extracting solution II；
S3: take the Angel wine high activity dried yeast of claret quality 0.05～0.1 ‰, by activating solution with dry after activation Extracting solution II described in extracting solution I and S2 described in red wine, S1 merges, after stirring under conditions of temperature is 15～20 DEG C Sealed fermenting 10～20 days, filter, obtain Abelmoschus manihot health care wine stock solution；
S4: added by L-arabinose in Abelmoschus manihot health care wine stock solution described in S3, adds purified water regulation alcoholic strength after stirring and dissolving Be 8～12 °, add clear stable agent, after stirring and evenly mixing temperature be-8～-4 DEG C under conditions of standing 10～15 days；
S5: filter, sterilizing, bottling, obtain Abelmoschus manihot health care wine of the present invention.
4. preparation method as claimed in claim 3, it is characterised in that in described step S1, the temperature of ultrasonic extraction be 70～ 85 DEG C, power is 450～800W.
5. preparation method as claimed in claim 3, it is characterised in that in described step S2, the volume fraction of ethanol be 60～ 80%.
6. preparation method as claimed in claim 3, it is characterised in that in described step S2, the temperature of ultrasonic extraction be 60～ 75 DEG C, power is 300～500W.
7. preparation method as claimed in claim 3, it is characterised in that in described step S3, the Angel dry ferment of wine high activity Female activation method be by Angel wine high activity dried yeast by liquid-solid ratio 18～22mL/g addition 38～40 DEG C contain crystal sugar 4～ The warm water of 8% stirs 25～40min, obtains activating solution.
8. preparation method as claimed in claim 3, it is characterised in that in described step S4, clear stable agent selected from bentonite, One or more in chitosan, gelatin, casein and kieselguhr.
9. preparation method as claimed in claim 8, it is characterised in that the addition of described clear stable agent is 0.2～0.4g/ L。
10. preparation method as claimed in claim 8, it is characterised in that described clear stable agent is chitosan and bentonitic Mixture, counts in mass ratio, chitosan: bentonite is 0.4～0.7:1.
CN201610481260.4A 2016-06-24 2016-06-24 Abelmoschus manihot health-care wine for preventing and treating diabetes and preparation method thereof Withdrawn CN106047543A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104673573A (en) * 2015-03-23 2015-06-03 孙燕霞 Wine with diabetes prevention and treatment function and preparation method thereof
CN105368654A (en) * 2015-09-24 2016-03-02 浙江秀地生物科技有限公司 Preparation method of okra rice wine

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104673573A (en) * 2015-03-23 2015-06-03 孙燕霞 Wine with diabetes prevention and treatment function and preparation method thereof
CN105368654A (en) * 2015-09-24 2016-03-02 浙江秀地生物科技有限公司 Preparation method of okra rice wine

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Application publication date: 20161026