CN106045587A - Preparation method of fertilizer containing microorganisms - Google Patents
Preparation method of fertilizer containing microorganisms Download PDFInfo
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- CN106045587A CN106045587A CN201610400417.6A CN201610400417A CN106045587A CN 106045587 A CN106045587 A CN 106045587A CN 201610400417 A CN201610400417 A CN 201610400417A CN 106045587 A CN106045587 A CN 106045587A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention relates to a preparation method of a fertilizer containing microorganisms. Plant residues, an acid-base regulating agent, distilled water, a culture medium and the microorganisms are added to a fermentation tank and fermented at certain temperature for certain time, a product is stored in the fermentation tank for 3-5 days after pH adjustment and cooling, slaked lime is added, pH is adjusted, the culture medium and beneficial microorganisms are continuously added to the fermentation tank and fermented at certain temperature, then, the product is stored in the fermentation tank for 3-5 days after pH adjustment and cooling, the process is repeated once, the product is air-dried and added to the fertilizer, and the fertilizer containing the microorganisms is obtained. The natural ecological environment of soil is simulated, fermentation is performed three times, a fermented microbial agent can rapidly adapt to the environment after being applied to the soil and the survival rate is ensured.
Description
Technical field
The present invention relates to the preparation method of a kind of fertilizer containing microorganism.
Background technology
The microbial inoculum quantity in the purity of microbial inoculum and unit mass is mainly included for the evaluation index of agricultural micro organism.Cause
This, the bacterial preparation process as prior art is usually: carry out once under the conditions of the temperature suitably survived in microorganism and pH
Fermentation technology, is equivalent to hot-house culture.
Owing to the acidifying of current soil compaction is serious, outside air temperature is changeable, own " indigenous " micro-life again in soil environment
Thing, the microbial bacterial agent of hot-house culture is directly rendered in soil, micro-because not adapting to " indigenous " in rugged environment and soil
The biological substantially all death of competition, surviving because forming population effect on a small quantity even if having, also cannot play microbial bacteria
The effect of agent.
Summary of the invention
The technical problem to be solved is to provide the preparation method of a kind of fertilizer containing microorganism.The method
Simulation soil natural ecological environment, makes the microbial inoculum after fermentation after putting into soil, can quickly adapt to environment, it is ensured that survival rate；And
Improve the struggle for existence ability of microbial inoculum.
In order to solve above-mentioned technical problem, the present invention by the following technical solutions:
The preparation method of a kind of fertilizer containing microorganism, it is characterised in that follow the steps below:
1), preparation product A: plant residue, acid-base modifier and water after drying according to mass ratio 1000:1～10:250～
500 join in fermentation tank, at 85 DEG C～130 DEG C of high temperature sterilizes 15～30min, obtain product A；
And prepare fermentation seed liquid: beneficial microbe is seeded to fluid medium, at 25 DEG C～40 DEG C, cultivates 20～30 little
Time, obtain fermentation seed liquid；The mass ratio of beneficial microbe and fluid medium is 2～15:100;
2), product B is prepared: join in product A according to the mass ratio 1～15:100 of seed liquor Yu product A by fermentation seed liquid,
Ferment 3～25 days under the conditions of 25 DEG C～40 DEG C, pH5～8, obtain product B；
3), preparation products C: adjust the pH to 5.0～5.5 of product B, and temperature in fermentation tank is cooled to 0 DEG C～8 DEG C, preserve 3
～5 days, then temperature is recovered to 25 DEG C～40 DEG C, obtain products C；
4), preparing product D: joined by fermentation seed liquid in products C, fermentation seed liquid is 1～15 with the mass ratio of products C:
100, at 25 DEG C～40 DEG C, ferment 3～25 days under the conditions of pH5～8, obtain product D；
5) fertilizer containing microorganism or product E, are prepared: product D obtains microbial bacterial agent after air-drying, the microorganism that will obtain
Microbial inoculum joins in fertilizer, obtains the fertilizer containing microorganism；
Or, adjust the pH to 5.0～5.5 of product D, and temperature in fermentation tank be cooled to 0 DEG C～8 DEG C, preserve 3～5 days,
Then temperature is recovered to 25 DEG C～40 DEG C, obtain product E；
6), preparation product F: being joined by fermentation seed liquid in product E, fermentation seed liquid is 1～15 with the mass ratio of product E:
100, at 25 DEG C～40 DEG C, ferment 3～25 days under the conditions of pH5～8, obtain product F；
7), microbial bacterial agent is prepared: product F obtains microbial bacterial agent after air-drying；
8), the preparation fertilizer containing microorganism: the microbial bacterial agent obtained is joined in fertilizer, obtains the fertilizer containing microorganism
Material；
Described plant residue is in Thallus Laminariae (Thallus Eckloniae), Entermorpha, bean cake, cottonseed meal, Testa Tritici, wheat stalk, corn straw, furfural dregs and mushroom residue
A kind of or arbitrary proportion two or more；
Described acid-base modifier is the two or more of a kind of or arbitrary proportion in plant ash, Calx, citric acid and oxalic acid；
Described beneficial microbe be two kinds of a kind of or arbitrary proportion in lactic acid bacteria, root nodule bacteria, bacillus cereus and yeast with
On.
Microbial bacterial agent described in step 5) and step 8) joins the feed postition of fertilizer and includes the most straight
Connect to join in fertilizer and after the interior addition manner of mixing and production, microbial bacterial agent is wrapped on fertilizer by the way of parcel
Outer addition manner.
In step 1), described plant residue is the Thallus Laminariae (Thallus Eckloniae) of mass ratio 2:5:3, bean cake and Testa Tritici；Acid-base modifier is ripe stone
Ash；The mass ratio of described plant residue, acid-base modifier and water is 1000:1:350；Sterilising temp 120 DEG C, sterilization time
20min。
In step 1), when preparing fermentation seed liquid, beneficial microbe is seeded in fluid medium, cultivates 26 at 35 DEG C
Hour, obtain fermentation seed liquid；The mass ratio of beneficial microbe and fluid medium is 8:100.
Step 2) in, seed liquor is 8:100 with the mass ratio of product A, ferments 8 days under the conditions of 35 DEG C, pH6.5～7.5.
In step 3) and step 5), in fermentation tank, temperature is cooled to 4 DEG C, preserves 4 days, recovers temperature to 35 DEG C.
The quality of fermentation seed liquid and product E in the mass ratio of fermentation seed liquid and products C and step 6) in step 4)
Ratio is 4:100；Step 4) and step 6), all at 35 DEG C, are fermented 8 days under the conditions of pH6.5～7.5.
Described beneficial microbe is bacillus subtilis.
Described fluid medium is made into according to following mass ratio: Carnis Bovis seu Bubali cream 1～10 parts, and peptone 1～10 parts are solvable
Property starch 1～10 parts, potassium nitrate 0.1～3 parts, dipotassium hydrogen phosphate 0.1～3 parts, magnesium sulfate 0.1～3 parts, ferrous sulfate
0.01～2 part, sodium chloride 0.1～3 parts, agar 5～25 parts, 1000 parts of water.
The present invention has a following Advantageous Effects:
1, the spawn culture simulation soil natural ecological environment of the present invention, makes microbial bacterial agent be applied in agricultural and is adapted to ring
Border, improves survival rate, preferably plays effect of microbial bacterial agent.
2, the present invention is by three fermentations, wherein can produce a small amount of miscellaneous bacteria, therefore in second time and third time sweat
Miscellaneous bacteria is controlled.If it exceeds three fermentations, miscellaneous bacteria controllability will become very poor.It addition, the present invention is supplemented by twice seed liquor,
Making probiotics quantity occupy absolute advantages and miscellaneous bacteria competes, the microbial inoculum turned out has stronger struggle for existence power, it is possible to effectively
Prevent from microbial bacterial agent from putting in soil to be killed by " indigenous " microorganism, play effect of microbial bacterial agent.
3, currently preferred bacillus acidophilus, has stronger acid resistance, and sweat is controlled.
4, microbial inoculum prepared by the present invention is in improvement soil, prevention and control disease, suppresses nematicide, improves crop quality, improves crop
The effect of the aspects such as yield is more prominent.
5, the present invention also has certain effect in terms of administering heavy metal pollution.
Detailed description of the invention
The present invention is further illustrated below in conjunction with instantiation.
Embodiment 1
Thallus Laminariae (Thallus Eckloniae), Entermorpha, Testa Tritici, Calx and distilled water joined fermentation tank according to mass ratio 200:500:300:1:350
In at 120 DEG C of high temperature sterilize 20min, obtain product A；And prepare fermentation fermentation seed liquid: bacillus subtilis is seeded to liquid
Body culture medium, at 35 DEG C, is cultivated 26 hours；Described fluid medium is: described fluid medium is: glucose 12g, egg
White peptone 4g, soluble starch 2g, potassium nitrate 0.6g, potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.8g, zinc sulfate 0.01g, sodium chloride
1g, agar 15g, distilled water 1000ml, pH6.8；The ratio of bacillus subtilis and fluid medium is 8:100;By fermentation kind
Sub-liquid joins in product A, and wherein fermentation seed liquid is 8:100 with the mass ratio of product A, at 35 DEG C, and pH6.5～7.5 conditions
Lower aerobic fermentation 8 days, obtains product B；Adjusting the pH to 5.0～5.5 of product B, cooling and fermentation tank temperature, to 4 DEG C, preserves 3 days,
Temperature is recovered to 35 DEG C, obtains products C；Fermentation seed liquid is joined in products C, wherein fermentation seed liquid and products C
Mass ratio is 4:100, at 35 DEG C, and aerobic fermentation 8 days under the conditions of pH6.5～7.5, obtain product D；Adjust the pH to 5.0 of product D
～5.5, cooling and fermentation tank temperature, to 4 DEG C, preserves 3 days, recovers temperature to 35 DEG C, obtains product E；Fermentation seed liquid is added
In product E, wherein the mass ratio of fermentation seed liquid and product E is 4:100, at 35 DEG C, and under the conditions of pH6.5～7.5 aerobic
Ferment 8 days, obtains product F；Product F obtains microbial bacterial agent after air-drying, and joins in fertilizer by the microbial bacterial agent obtained, to obtain final product
Fertilizer containing microorganism.
Embodiment 2
Thallus Laminariae (Thallus Eckloniae), Entermorpha, Testa Tritici, Calx and distilled water joined fermentation tank according to mass ratio 200:500:300:1:350
In at 130 DEG C of high temperature sterilize 15min, obtain product A；And prepare fermentation fermentation seed liquid: by Azospirillum brasilense and hay bud
Spore bacillus is seeded to fluid medium at 35 DEG C, cultivates 26 hours；Described fluid medium is: described fluid medium is:
Glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.8g, zinc sulfate
0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8；Azospirillum brasilense, bacillus subtilis and liquid culture
The ratio of base is 4:4:100;Being joined by fermentation seed liquid in product A, wherein fermentation seed liquid is 8 with the mass ratio of product A:
100, at 35 DEG C, under the conditions of pH6.5～7.5, aerobic fermentation 6 days, obtain product B；Adjust the pH to 5.0～5.5 of product B, cold
But fermentation jar temperature is to 4 DEG C, preserves 3 days, recovers temperature to 35 DEG C, obtains products C；Fermentation seed liquid is joined products C
In, wherein fermentation seed liquid is 4:100 with the mass ratio of products C, at 35 DEG C, and aerobic fermentation 6 days under the conditions of pH6.5～7.5,
To product D；Adjusting the pH to 5.0～5.5 of product D, cooling and fermentation tank temperature, to 4 DEG C, preserves 3 days, recovers temperature to 35 DEG C,
Obtain product E；Being joined by fermentation seed liquid in product E, wherein fermentation seed liquid is 4:100 with the mass ratio of product E, 35
DEG C, under the conditions of pH6.5～7.5, aerobic fermentation 6 days, obtain product F；Product F obtains microbial bacterial agent after air-drying, micro-by obtain
Bacteria agent joins in fertilizer, obtains the fertilizer containing microorganism.
Embodiment 3
Thallus Laminariae (Thallus Eckloniae), Entermorpha, Testa Tritici, Calx and distilled water joined fermentation tank according to mass ratio 200:500:300:2:300
In at 115 DEG C of high temperature sterilize 25min, obtain product A；And prepare fermentation fermentation seed liquid: bacillus subtilis is seeded to liquid
Body culture medium, at 30 DEG C, is cultivated 26 hours；Described fluid medium is: described fluid medium is: glucose 12g, egg
White peptone 4g, soluble starch 2g, potassium nitrate 0.6g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.8g, zinc sulfate 0.01g, sodium chloride
1g, agar 15g, distilled water 1000ml, pH6.8；The ratio of bacillus subtilis and fluid medium is 8:100;
Being joined by fermentation seed liquid in product A, wherein fermentation seed liquid is 8:100 with the mass ratio of product A, at 30 DEG C,
Under the conditions of pH6.5～7.5, aerobic fermentation 5 days, obtain product B；Adjust the pH to 5.0～5.5 of product B, cooling and fermentation tank temperature
To 5 DEG C, preserve 3 days, temperature is recovered to 30 DEG C, obtains products C；Fermentation seed liquid is joined in products C, kind of wherein fermenting
Sub-liquid is 4:100 with the mass ratio of products C, at 30 DEG C, and aerobic fermentation 5 days under the conditions of pH6.5～7.5, obtain product D；Adjust
The pH of product D to 5.0～5.5, cooling and fermentation tank temperature, to 5 DEG C, preserves 3 days, recovers temperature to 30 DEG C, obtains product E；Will
Fermentation seed liquid joins in product E, and wherein the mass ratio of fermentation seed liquid and product E is 4:100, at 30 DEG C, pH6.5～
Under the conditions of 7.5, aerobic fermentation 5 days, obtain product F；Product F obtains microbial bacterial agent after air-drying, and is added by the microbial bacterial agent obtained
Enter in fertilizer, obtain the fertilizer containing microorganism.
Embodiment 4
Adding Thallus Laminariae (Thallus Eckloniae), Entermorpha, Testa Tritici, Calx, citric acid and distilled water according to mass ratio 200:500:300:1:1:350
At 95 DEG C of high temperature sterilize 30min in fermentation tank, obtain product A；And prepare fermentation fermentation seed liquid: by bacillus subtilis
It is seeded to fluid medium at 40 DEG C, cultivates 24 hours；Described fluid medium is: described fluid medium is: glucose
12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.8g, zinc sulfate 0.01g,
Sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8；The ratio of bacillus subtilis and fluid medium is 5:100;Will
Fermentation seed liquid joins in product A, and wherein the mass ratio of fermentation seed liquid and product A is 8:100, at 40 DEG C, pH6.5～
Under the conditions of 7.5, aerobic fermentation 10 days, obtain product B；Adjust product B pH to 5.0～5.5, cooling and fermentation tank temperature to 4 DEG C,
Preserve 3 days, temperature is recovered to 40 DEG C, obtains products C；Fermentation seed liquid is joined in products C, wherein fermentation seed liquid with
The mass ratio of products C is 4:100, at 40 DEG C, and aerobic fermentation 8 days under the conditions of pH6.5～7.5, obtain product D；Adjust product D's
PH to 5.0～5.5, cooling and fermentation tank temperature, to 4 DEG C, preserves 3 days, recovers temperature to 40 DEG C, obtains product E；By fermentation kind
Sub-liquid joins in product E, and wherein fermentation seed liquid is 4:100 with the mass ratio of product E, at 40 DEG C, and pH6.5～7.5 conditions
Lower aerobic fermentation 8 days, obtains product F；Product F obtains microbial bacterial agent after air-drying, and the microbial bacterial agent obtained is joined fertilizer
In material, obtain the fertilizer containing microorganism.
Embodiment 5
Additional embodiment (two-step method)
Further illustrate below in conjunction with experimental data and the method have the benefit that
1 materials and methods:
1.1 test sites: Weifang Shouguang City, neutral brown earth.
1.2 variety of watermelon: black beauty.
1.3 for examination fertilizer: for the common fertilizer containing microorganism (for one time fermentation, other manufacture method and embodiment 1
Identical), embodiment 1, embodiment 2, embodiment 3 and embodiment 4 do effectiveness comparison.
1.4 for examination plot: upper one season crop be black beauty's Citrullus vulgaris, genus second crop soil.
1.5 EXPERIMENTAL DESIGN: test sets 5 process, repeats for 2 times, Gong10Ge community, totally 2 groups, each community random alignment.Little
District area 60m2, use mechanical planting, 80 black beauty's Citrullus vulgariss of every community sowing.Every community base respectively is executed common containing microorganism
Fertilizer (for one time fermentation, other manufacture method is same as in Example 1), embodiment 1, embodiment 2, embodiment 3 and embodiment
4, community list contracture does not calculate yield.
On May 12nd, 2014 is sowed, and on August 21st, 2014 gathers in the crops, and this experiment other management in addition to experiment fertilizer difference are equal
Unanimously.
2 results and analysis
Embodiment 1, embodiment 2, embodiment 3 and embodiment 4 and the common fertilizer containing microorganism are (for one time fermentation, other system
Make method same as in Example 1) process and compare, embodiment 1, embodiment 2, embodiment 3 and embodiment 4 watermelon growing gesture are strong, root
System's prosperity, tolerance to cold is strong, Ye Senong, and pest and disease damage is light, owing to experimental plot is continuous cropping field, finds experimental station on June 15th, 2014
Pitchy fleck occur on the blade that the Citrullus vulgaris of reason has, June 22, black splotch became diameter 1～the brown patch of 2 centimetres, brown patch
For circular or irregular cycle.July 5 find climing upper generation water soaking mode scab, it is withered that central authorities become brown, and brown part in
Starlike dry and cracked, inside, in cork shape dry rot, is identified as watermelon grafting, and Citrullus vulgaris of falling ill each community is checked, and checks number
According to such as table 1:
Table 1
As can be seen from Table 1, product of the present invention is substantially better than one time fermentation product at anti-continuous cropping, disease resistance aspect.
Each community the yield of watermelon is done statistical result such as table 2:
Table 2
Embodiment 1 rate of growth is the highest as can be seen from Table 2, and effect is best.Comprehensive Tables 1 and 2 result draws, with common containing micro-
Biological fertilizer is compared, and it is strong that the present invention has disease-resistant performance, the obvious advantage of output increased.
Discuss: the purity emphasizing probiotics that at present bacterial manure is too much and quantity, and ignore suitable in soil of probiotics
Ying Xinghe and the resistance property of pathogenic bacteria, a group of sheep and ten wolf effects are different, think personally that the future of agriculture is heavy with bacterium research
Point is to improve agricultural adaptability and the resistance property of bacterium.
Claims (9)
1. the preparation method of the fertilizer containing microorganism, it is characterised in that follow the steps below:
1), preparation product A: plant residue, acid-base modifier and water after drying according to mass ratio 1000:1～10:250～
500 join in fermentation tank, at 85 DEG C～130 DEG C of high temperature sterilizes 15～30min, obtain product A；
And prepare fermentation seed liquid: beneficial microbe is seeded to fluid medium, at 25 DEG C～40 DEG C, cultivates 20～30 little
Time, obtain fermentation seed liquid；The mass ratio of beneficial microbe and fluid medium is 2～15:100;
2), product B is prepared: join in product A according to the mass ratio 1～15:100 of seed liquor Yu product A by fermentation seed liquid,
Ferment 3～25 days under the conditions of 25 DEG C～40 DEG C, pH5～8, obtain product B；
3), preparation products C: adjust the pH to 5.0～5.5 of product B, and temperature in fermentation tank is cooled to 0 DEG C～8 DEG C, preserve 3
～5 days, then temperature is recovered to 25 DEG C～40 DEG C, obtain products C；
4), preparing product D: joined by fermentation seed liquid in products C, fermentation seed liquid is 1～15 with the mass ratio of products C:
100, at 25 DEG C～40 DEG C, ferment 3～25 days under the conditions of pH5～8, obtain product D；
5) fertilizer containing microorganism or product E, are prepared: product D obtains microbial bacterial agent after air-drying, the microorganism that will obtain
Microbial inoculum joins in fertilizer, obtains the fertilizer containing microorganism；
Or, adjust the pH to 5.0～5.5 of product D, and temperature in fermentation tank be cooled to 0 DEG C～8 DEG C, preserve 3～5 days,
Then temperature is recovered to 25 DEG C～40 DEG C, obtain product E；
6), preparation product F: being joined by fermentation seed liquid in product E, fermentation seed liquid is 1～15 with the mass ratio of product E:
100, at 25 DEG C～40 DEG C, ferment 3～25 days under the conditions of pH5～8, obtain product F；
7), microbial bacterial agent is prepared: product F obtains microbial bacterial agent after air-drying；
8), the preparation fertilizer containing microorganism: the microbial bacterial agent obtained is joined in fertilizer, obtains the fertilizer containing microorganism
Material；
Described plant residue is in Thallus Laminariae (Thallus Eckloniae), Entermorpha, bean cake, cottonseed meal, Testa Tritici, wheat stalk, corn straw, furfural dregs and mushroom residue
A kind of or arbitrary proportion two or more；
Described acid-base modifier is the two or more of a kind of or arbitrary proportion in plant ash, Calx, citric acid and oxalic acid；
Described beneficial microbe be two kinds of a kind of or arbitrary proportion in lactic acid bacteria, root nodule bacteria, bacillus cereus and yeast with
On.
2. the preparation method of the fertilizer containing microorganism as claimed in claim 1, it is characterised in that: in step 5) and step 8)
Described microbial bacterial agent joins in the feed postition of fertilizer includes being added directly in fertilizer mixing in process of production and adds
The outer addition manner after add mode and production, microbial bacterial agent being wrapped on fertilizer by the way of parcel.
3. the preparation method of the fertilizer containing microorganism as claimed in claim 1, it is characterised in that:
In step 1), described plant residue is the Thallus Laminariae (Thallus Eckloniae) of mass ratio 2:5:3, bean cake and Testa Tritici；Acid-base modifier is Calx；Institute
The mass ratio stating plant residue, acid-base modifier and water is 1000:1:350；Sterilising temp 120 DEG C, sterilization time 20min.
4. the preparation method of the fertilizer containing microorganism as claimed in claim 1, it is characterised in that:
In step 1), when preparing fermentation seed liquid, beneficial microbe is seeded in fluid medium, cultivates 26 little at 35 DEG C
Time, obtain fermentation seed liquid；The mass ratio of beneficial microbe and fluid medium is 8:100.
5. the preparation method of the fertilizer containing microorganism as claimed in claim 1, it is characterised in that:
Step 2) in, seed liquor is 8:100 with the mass ratio of product A, ferments 8 days under the conditions of 35 DEG C, pH6.5～7.5.
6. the preparation method of the fertilizer containing microorganism as claimed in claim 1, it is characterised in that:
In step 3) and step 5), in fermentation tank, temperature is cooled to 4 DEG C, preserves 4 days, recovers temperature to 35 DEG C.
7. the preparation method of the fertilizer containing microorganism as claimed in claim 1, it is characterised in that:
In step 4), fermentation seed liquid is equal with the mass ratio of product E with fermentation seed liquid in the mass ratio of products C and step 6)
For 4:100；Step 4) and step 6), all at 35 DEG C, are fermented 8 days under the conditions of pH6.5～7.5.
8. the preparation method of the fertilizer containing microorganism as described in claim 1 to 7 any one, it is characterised in that:
Described beneficial microbe is bacillus subtilis.
9. the preparation method of the fertilizer containing microorganism as described in claim 1 to 7 any one, it is characterised in that:
Described fluid medium is made into according to following mass ratio: Carnis Bovis seu Bubali cream 1～10 parts, peptone 1～10 parts, and solubility is formed sediment
Powder 1～10 parts, potassium nitrate 0.1～3 parts, dipotassium hydrogen phosphate 0.1～3 parts, magnesium sulfate 0.1～3 parts, ferrous sulfate 0.01
～2 parts, sodium chloride 0.1～3 parts, agar 5～25 parts, 1000 parts of water.
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| CN201610400417.6A CN106045587A (en) | 2016-06-08 | 2016-06-08 | Preparation method of fertilizer containing microorganisms |
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|---|---|---|---|
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352329A (en) * | 2011-09-28 | 2012-02-15 | 重庆泰平药业有限公司 | Production method for microbial germ powder |
| CN103787708A (en) * | 2013-12-22 | 2014-05-14 | 宾阳县明翔新材料科技有限公司 | Method for producing straw decomposition agent by utilizing filter mud, cassava residues and waste alcohol mash |
-
2016
- 2016-06-08 CN CN201610400417.6A patent/CN106045587A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352329A (en) * | 2011-09-28 | 2012-02-15 | 重庆泰平药业有限公司 | Production method for microbial germ powder |
| CN103787708A (en) * | 2013-12-22 | 2014-05-14 | 宾阳县明翔新材料科技有限公司 | Method for producing straw decomposition agent by utilizing filter mud, cassava residues and waste alcohol mash |
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Application publication date: 20161026 |