CN106039404A - Preparation method for extracellular matrix (ECM) composite biological patch - Google Patents
Preparation method for extracellular matrix (ECM) composite biological patch Download PDFInfo
- Publication number
- CN106039404A CN106039404A CN201610213295.XA CN201610213295A CN106039404A CN 106039404 A CN106039404 A CN 106039404A CN 201610213295 A CN201610213295 A CN 201610213295A CN 106039404 A CN106039404 A CN 106039404A
- Authority
- CN
- China
- Prior art keywords
- preparation
- layer
- extracellular matrix
- laying
- evacuation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3633—Extracellular matrix [ECM]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Botany (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- General Chemical & Material Sciences (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention relates to a preparation method for an ECM composite biological patch. Specifically, the preparation method comprises the following steps: partially overlapping a plurality of ECM units along a transverse or longitudinal direction to form a layer and partially overlapping a plurality of ECM units along a direction perpendicular to the layer in sequence so as to form a second layer; repeating the previous step as needed until a desired layer number is obtained; and then subjecting the laid material to vacuumized lamination so as to obtain the composite biological patch. The composite biological patch prepared by using the method has improved tensile strength and can reduce consumption of ECM.
Description
Technical field
The present invention relates to the preparation method of a kind of extracellular matrix compound bio sticking patch.
Background technology
Extracellular matrix (extracellular matrix, ECM) is kind of a complicated dynamical system, and it is not only dimension
Hold biological support necessary to tissue and organ, avoid tissue and organ to be dissolved into a pasty state, can also release in addition
Put important signaling molecule, regulation and control body carries out self-regeneration.The ECM biomaterial master applied in the market
Small intestinal submucosa to be included (small intestinal submucosa, SIS), bladder mucosa layer basement membrane (urinary
Bladder membrane, UBM), amniotic membrane, corium, pericardium, peritoneum etc., have with its biological sticking patch made
The advantages such as, foreign reaction good with the human-body biological compatibility.
Rebuild due to hernia and repair materials is all had certain position stretch wanting of intensity and size by the disease such as cerebral dura mater reparation
Ask, and its tensile strength of monolithic ECM (such as SIS and UBM) biomaterial and size far can not meet demand,
If this is accomplished by reaching certain size and intensity, to meet clinical demand with dry tablet ECM Material cladding molding.
Report that some ECM biomaterials are combined method of superposition (such as CN 103877619 A), but its material weight
It is strong to product stretching that folded too much (more than 1cm) easily causes product local technique uneven substantially, composite molding
The most limited and to biomaterial the loss of increase of degree is the most more serious, and large-scale production in the future, to economic benefit
There is certain impact.
Summary of the invention
The present invention relates to the preparation method of a kind of extracellular matrix compound bio sticking patch, described method includes walking as follows
Rapid:
1) placement that partly overlapped successively by multiple extracellular matrix unit X in one direction forms one and lays layer A；
2) repeat step 1 along the direction Y vertical with X above layer A laying) step obtain laying layer B；
3) as required according to the number of plies of abovementioned steps paving to needs；
4) then the material evacuation laid lamination is obtained extracellular matrix compound bio sticking patch；
Wherein, in step 1), 2) described in overlapping part width be 0.1 to 1cm.
Described step 1) laying can carry out on flat board, step 3) complete after can spread on paver
If one flat plate；Flat board in preferably two steps is bakelite material.Described flat board can run through with multiple
Hole, preferably hole be shaped as square, rhombus or circle.
In a preferred embodiment of the present invention, it is covered with hospital gauze between described flat board and extracellular matrix.
In a preferred embodiment of the present invention, step 3) in the number of plies be 2 to 20 layers.
In presently preferred embodiment, step 1) complete the once-paving of X-direction after, root
According to needing the multiple laying layer A of laying that partly overlap successively along perpendicular Y-direction, the wherein overlap of Y-direction
Position width is 0.1 to 1cm.
In presently preferred embodiment, step 2) complete the once-paving of Y-direction after, root
According to needing the multiple laying layer B of laying that partly overlap successively along perpendicular X-direction, the wherein overlap of X-direction
Position width is 0.1 to 1cm.
Step 4) in evacuation lamination method have no particular limits, in yet other embodiments
In, with holes including the two sides laid is placed in a side with the cell epimatrix material of bakelite plate
In valve bag, the then step of evacuation；The most described valve bag is for thickening valve bag；The most described hole is certainly
Envelope side central authorities.The big I wherein thickening valve bag is determined according to the size of bakelite plate, the most permissible
From No. 1 (6*8.5cm) to No. 12 (45*34cm).
In a preferred embodiment of the present invention, sustainable 2-180 minute of the time of evacuation.
In presently preferred embodiment, after evacuation lamination, obtain extracellular matrix compound bio
Sew up with absorbable thread after sticking patch is lyophilised.Lyophilizing operation can be in-10～-80 DEG C, pre-more than lyophilizing 2h, then adjust
Joint vacuum pressure < 300Pa (preferably < 50Pa), more than lyophilizing 4h.
The preparation method of the present invention may additionally include between any two ECM unit or two laying interlayers add bonding
The step of agent.Wherein, described binding agent include but not limited to Fibrin Glue (concentration can be 30～150g/L),
Sodium alginate (concentration can be 0.1%～3%) etc..Between two-layer ECM or two lay appropriate the adding of interlayer
Enter above-mentioned binding agent and can strengthen the interaction between multilamellar ECM.
In a preferred embodiment of the present invention, after also including individually having laid laying layer A or laying layer B
The step of evacuation lamination, alternately lays after these single layer A or layer B evacuation being laminated again, has laid
Vacuum lamination again after one-tenth, can further improve the intensity of the compound bio sticking patch finally completed.
In the preparation process in accordance with the present invention, described cell epimatrix material is not particularly limited, preferably SIS or
UBM。
Present invention also offers a kind of compound bio sticking patch prepared by said method.
Heretofore described X refers to the two and substantially becomes 90 degree with the vertical relation of Y-direction, but it is not intended that
It must be precise perpendicularity, as long as the anisotropy reducing product can be played, the stretching improving ECM is strong
The effect of degree, i.e. meets heretofore described " vertical relation ".
The biological sticking patch using the method for the present invention to be prepared as has the advantage, that between (1) each ECM unit
Compound tight, and product hot strength is high, and (hot strength before stitching is more than 85N, the hot strength after stitching
More than 105N)；(2) compound overlapping part is short, saves material, and large-scale production can produce bigger economic effect
Benefit；(3) the material entirety uniformity after being combined is good.
Accompanying drawing explanation
Fig. 1 be the embodiment of the present invention 1 Small Intestine tissue longitudinally, laterally；
Fig. 2 is that in the embodiment of the present invention 1, in each laying layer, SIS lays pattern；
Fig. 3 is the laying pattern (being 3 row in figure) of multiple row SIS layer A in the embodiment of the present invention 1；
Fig. 4 is that in the embodiment of the present invention 1, adjacent laying interlayer lays pattern.
Detailed description of the invention
It is used for further describing the present invention below in conjunction with embodiment, but these embodiments the model of the unrestricted present invention
Enclose.
Embodiment 1
(1) preparation of SIS or UBM: pig small intestine or bladder obtain SIS or UBM material after mechanical treatment,
After peracetic acid+ethanol disinfection cleaning, obtain SIS or UBM.
A, SIS is longitudinally cut open along intestinal tube, and transversely SIS is cut into the SIS of 5-30cm longitudinal length
Unit；
B, UBM are not required to cutting, directly arrive next step (the same SIS of remaining method).
(2) making of vacuum lamination apparatus: vacuum pump rubber tube is connected a pair of mouth bottle,suction one end,
And bottle,suction is placed in equipped with in the bubble chamber of dry ice, stand-by.
(3) take A4 paper size (30*21cm) bakelite material flat board and be evenly distributed with many through holes
(pattern has square, rhombus, circle etc.)] be laid on table top, and on one layer of flat board upper berth medical husky cloth or
Do not spread, the left upper end of medical husky cloth will be laid in by first piece of SIS unit of gained in (1), by second SIS
Unit and first SIS cell mesh overlap are placed, and the certain distance that staggers along the longitudinal direction, it is ensured that two SIS
Unit horizontally-parallel, the lap width of two SIS unit between 0.1-1cm, the 3rd SIS unit
Place along longitudinal direction before and second SIS cell mesh overlap, make the horizontally-parallel of two SIS unit equally,
The lap width 0.1-1cm of two SIS unit, is repeated in, until SIS in terms of left upper end turns left lower end
(if need to spread secondary series, two SIS unit are in addition to needing longitudinal overlap 0.1-1cm, laterally to be paved with whole corrosion resistant plate
Also overlapping 0.1-1cm is needed), obtain SIS layer A；
(4) the SIS layer A half-twist angle on table top that will complete, the same step of method (4), separately take SIS
Unit starts overlapping laying along the SIS layer A left upper end edge after half-twist, until being paved with whole bakelite
Plate, obtains SIS layer B；
(5) repeat the above steps (4) and (5) are laid to the required number of plies (2-20 layer) as required；
(6) it is layered in the number of plies set with one piece of medical husky cloth again, then presses another block band in the above and run through
The bakelite plate in hole, and laying absorbent filter or absorbent cotton on husky cloth.
(7) manufacture method of lamination bag: take No. 10 (24*34cm) and thicken valve bag, in its side
A centre bore is bored in centre, and by one end little for a mini-funnels (such as: survey vital capacity blow gun) in valve bag outward
Through centre bore.
(8) the bakelite plate completed in (6) is put into the lamination bag in (7), and by middle-size and small-size to (7)
Funnel tips upside down on last layer bakelite plate.
(9) spout of funnel exposed in (8) is connected to the other end of bottle,suction in (2) by rubber tube.Open
Dynamic vacuum pump, starts laminating technology, persistent period 2min-12h.
Embodiment 2
Prepare the semi-finished product in (9) according to the mode of embodiment 1, with freeze dryer lyophilizing (-50 DEG C, vacuum
Under the conditions of 50 μ bar, lyophilizing 16 hours), by stitching tough machine by the absorbable linear slit of product middle after lyophilizing after lyophilizing
Close.
Embodiment 3
Take an A4 paper size bakelite plate, use SIS to prepare compound bio sticking patch wherein by embodiment 1 mode
Totally 6 layers of A layer and B layer (length of overlapped part is 0.8cm), lamination be placed on freeze dryer (-50 DEG C, vacuum 50 μ bar
Under the conditions of, lyophilizing 16 hours), obtain SIS biological sticking patch finished product.(recording hot strength with tension tester is
52N)。
Embodiment 4
Take a B5 paper size polyethylene board, use SIS to prepare compound bio sticking patch wherein by by embodiment 1 mode
Totally 4 layers of A layer and B layer (length of overlapped part is 0.8cm), gluing compound 4 layers, start laminating technology,
4 layers of UBM biological sticking patch of a composite molding, be placed in freeze dryer (-45 DEG C, under the conditions of vacuum 20 μ bar, lyophilizing
12 hours), obtain UBM biological sticking patch finished product.(recording hot strength with tension tester is 90N).
Embodiment 5
Take a B5 paper size polyethylene board, use SIS to prepare compound bio sticking patch wherein by by embodiment 1 mode
Totally 12 layers of A layer and B layer (length of overlapped part is 0.9cm), gluing compound 12 layers, start laminating technology,
12 layers of UBM biological sticking patch of a composite molding,
Dry 12 hours), after lyophilizing, gained semi-finished product sew up arbitrary graphic pattern through 4-0 absorbable thread, obtain UBM biological sticking patch
Finished product.(recording hot strength with tension tester is 110N).
Claims (14)
1. a preparation method for extracellular matrix compound bio sticking patch, described method comprises the steps:
1) placement that partly overlapped successively by multiple extracellular matrix unit X in one direction forms one and lays layer A；
2) repeat step 1 along the direction Y vertical with X above layer A laying) step obtain laying layer B；
3) abovementioned steps paving is repeated as required to the number of plies needed；
4) then the material evacuation laid lamination is obtained extracellular matrix compound bio sticking patch；
Wherein, in step 1), 2) described in overlapping part width be 0.1 to 1cm.
Preparation method the most according to claim 1, wherein said step 1) be laid on flat board and carry out,
Step 3) complete after above paver lay one flat plate；Flat board in preferably two steps is selected from phenolic aldehyde
One or both in plastics, polyethylene, epoxy resin, polymethyl methacrylate.
Preparation method the most according to claim 2, wherein said flat board is with multiple through holes, preferably hole
Be shaped as square, rhombus or circle.
Preparation method the most according to claim 2, is covered with between wherein said flat board and extracellular matrix
Hospital gauze.
Preparation method the most according to claim 1, wherein step 3) in the number of plies be 2 to 20 layers.
Preparation method the most according to claim 1, wherein step 1) complete the once-paving of X-direction after,
Partly overlap successively laying multiple laying layer A as required along perpendicular Y-direction, the wherein weight of Y-direction
Folded position width is 0.1 to 1cm.
Preparation method the most according to claim 1, wherein step 2) complete the once-paving of Y-direction after,
Partly overlap successively laying multiple laying layer B as required along perpendicular X-direction, the wherein weight of X-direction
Folded position width is 0.1 to 1cm.
Preparation method the most according to claim 1, wherein said step 4) evacuation lamination include by
The two sides laid is placed in a side valve bag with holes with the cell epimatrix material of bakelite plate,
Then the step of evacuation；The most described valve bag is for thickening valve bag；The most described hole is in valve bag side
Centre.
Preparation method the most according to claim 8, wherein said evacuation continues 2-180 minute.
Preparation method the most according to claim 1, obtains extracellular matrix after also including evacuation lamination
The step sewed up with absorbable thread after compound bio sticking patch is lyophilised.
11. preparation methoies according to claim 1, it is characterised in that be additionally included in any two ECM mono-
Between unit or two lay interlayer add binding agents steps, the most described binding agent is Fibrin Glue or alginic acid
Sodium.
12. preparation methoies according to claim 1, it is characterised in that also include laying layer A or laying
The step of layer B independent evacuation lamination.
13. preparation methoies according to claim 1, described extracellular matrix is selected from SIS or UBM.
14. 1 kinds of biological benefits prepared by the preparation method as described in claim 1-13 any one
Sheet.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2015101619672 | 2015-04-08 | ||
| CN201510161967 | 2015-04-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106039404A true CN106039404A (en) | 2016-10-26 |
Family
ID=57484265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610213295.XA Pending CN106039404A (en) | 2015-04-08 | 2016-04-07 | Preparation method for extracellular matrix (ECM) composite biological patch |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106039404A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107233624A (en) * | 2017-06-16 | 2017-10-10 | 卓阮医疗科技（苏州）有限公司 | A kind of splicing biomaterial of two-way mechanics uniform intensity and preparation method thereof |
| CN107320774A (en) * | 2017-06-16 | 2017-11-07 | 卓阮医疗科技（苏州）有限公司 | One kind splicing biomaterial and preparation method thereof |
| CN108126241A (en) * | 2018-03-01 | 2018-06-08 | 北京迈迪顶峰医疗科技有限公司 | Tissue repair sticking patch, main body and preparation method |
| CN110464505A (en) * | 2019-07-12 | 2019-11-19 | 上海大学 | A kind of enhanced reticular structure stomach wall of elasticity repairs sticking patch and preparation method thereof |
| CN113713168A (en) * | 2021-09-02 | 2021-11-30 | 上海大学 | Abdominal wall repairing patch and preparation method thereof |
| CN113908343A (en) * | 2021-09-30 | 2022-01-11 | 卓阮医疗科技（苏州）有限公司 | Biomaterial with improved bending performance |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012112441A1 (en) * | 2011-02-14 | 2012-08-23 | Mimedx Group Inc. | Laminated tissue grafts composed of wharton's jelly and methods of making and using the same |
| CN103251987A (en) * | 2013-04-07 | 2013-08-21 | 陕西佰傲再生医学有限公司 | Acellular biological patch, preparation method and apparatus thereof |
| CN103877619A (en) * | 2014-01-24 | 2014-06-25 | 北京大清生物技术有限公司 | Compound SIS tissue repair material and preparation method thereof |
| WO2014138309A1 (en) * | 2013-03-06 | 2014-09-12 | Awod, Inc., A California Corporation | Chronic wound dressing with variable pore sizes |
-
2016
- 2016-04-07 CN CN201610213295.XA patent/CN106039404A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012112441A1 (en) * | 2011-02-14 | 2012-08-23 | Mimedx Group Inc. | Laminated tissue grafts composed of wharton's jelly and methods of making and using the same |
| WO2014138309A1 (en) * | 2013-03-06 | 2014-09-12 | Awod, Inc., A California Corporation | Chronic wound dressing with variable pore sizes |
| CN103251987A (en) * | 2013-04-07 | 2013-08-21 | 陕西佰傲再生医学有限公司 | Acellular biological patch, preparation method and apparatus thereof |
| CN103877619A (en) * | 2014-01-24 | 2014-06-25 | 北京大清生物技术有限公司 | Compound SIS tissue repair material and preparation method thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107233624A (en) * | 2017-06-16 | 2017-10-10 | 卓阮医疗科技（苏州）有限公司 | A kind of splicing biomaterial of two-way mechanics uniform intensity and preparation method thereof |
| CN107320774A (en) * | 2017-06-16 | 2017-11-07 | 卓阮医疗科技（苏州）有限公司 | One kind splicing biomaterial and preparation method thereof |
| CN107320774B (en) * | 2017-06-16 | 2020-04-07 | 卓阮医疗科技（苏州）有限公司 | Spliced biological material and preparation method thereof |
| CN108126241A (en) * | 2018-03-01 | 2018-06-08 | 北京迈迪顶峰医疗科技有限公司 | Tissue repair sticking patch, main body and preparation method |
| CN110464505A (en) * | 2019-07-12 | 2019-11-19 | 上海大学 | A kind of enhanced reticular structure stomach wall of elasticity repairs sticking patch and preparation method thereof |
| CN113713168A (en) * | 2021-09-02 | 2021-11-30 | 上海大学 | Abdominal wall repairing patch and preparation method thereof |
| CN113713168B (en) * | 2021-09-02 | 2022-07-29 | 上海大学 | Abdominal wall repairing patch and preparation method thereof |
| CN113908343A (en) * | 2021-09-30 | 2022-01-11 | 卓阮医疗科技（苏州）有限公司 | Biomaterial with improved bending performance |
| CN113908343B (en) * | 2021-09-30 | 2023-01-20 | 卓阮医疗科技（苏州）有限公司 | Biomaterial with improved bending performance |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106039404A (en) | Preparation method for extracellular matrix (ECM) composite biological patch | |
| CA3099695A1 (en) | 2d and 3d bioscaffold extracellular structural unit and tissue structure design and methods of manufacture | |
| CN101015709B (en) | Dural graft and method of preparing the same | |
| CN104096268B (en) | A kind of mineralized collagen artificial periosteum and preparation method thereof | |
| CN108478866B (en) | Tissue repair scaffold, preparation method and application thereof | |
| CN107050519B (en) | Preparation method of multilayer absorbable biological membrane | |
| JP2000508202A (en) | Porous submucosal tissue graft construct | |
| WO2017088818A1 (en) | Tissue repair fiber membrane, preparation method and application thereof, and tissue repair product | |
| DE102006042732B3 (en) | Artificial capillary membrane system for wound treatment, comprises a capillary hollow fiber membrane system having a common inlet and outlet connected with a pumping device and a fluid media feed pump arranged in the capillary system | |
| CN107823714A (en) | For the formation system for preparing tissue engineered bone cartilage frame and biological 3D printing manufacturing process | |
| CN107854727B (en) | Biological tendon repair material and preparation method thereof | |
| CN106267347A (en) | Biological sticking patch and preparation method thereof at the bottom of basin | |
| CN203220462U (en) | Perforated biological patch | |
| CN106132450A (en) | The pleura substrate of decellularization | |
| CN103877619A (en) | Compound SIS tissue repair material and preparation method thereof | |
| CN107405425A (en) | It is laminated tissue transplantation product | |
| CN106039405A (en) | Preparation method for extracellular matrix (ECM) composite biological patch | |
| CN1830492B (en) | Bioprotein artificial meninges sheet | |
| CN102908676A (en) | Hollowed PP/PVDF (Polypropylene/Polyvinylidene Fluoride) composite hernia sticking patch and preparation method thereof | |
| CN114225110B (en) | Collagen-based dura mater and preparation method thereof | |
| CN106901868B (en) | A kind of upper joint capsule rebuilds reparation sticking patch and preparation method thereof | |
| JP2000237300A (en) | Hollow yarn with fine diameter, its hollow yarn bundle and manufacture thereof | |
| CN106137455B (en) | A kind of cranium face, nose repair sticking patch | |
| CN206228466U (en) | A kind of cranium face, nose repair sticking patch | |
| CN107898489A (en) | One kind skin-grafting packing fixed structure and fixing means |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161026 |
|
| RJ01 | Rejection of invention patent application after publication |