CN103877619A - Compound SIS tissue repair material and preparation method thereof - Google Patents
Compound SIS tissue repair material and preparation method thereof Download PDFInfo
- Publication number
- CN103877619A CN103877619A CN201410035832.7A CN201410035832A CN103877619A CN 103877619 A CN103877619 A CN 103877619A CN 201410035832 A CN201410035832 A CN 201410035832A CN 103877619 A CN103877619 A CN 103877619A
- Authority
- CN
- China
- Prior art keywords
- sis
- laying
- layer
- unit
- lay
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention provides a compound SIS tissue repair material and a preparation method thereof. The SIS is stacked according to a special laying manner, is clamped and pressed by an upper splint and a lower splint with holes, and then is dried so as to obtain a large-size SIS lamellar structure repair material, which has tightly combined layers and is bidirectionally isotropic in mechanics. The problem that the SIS is limited by the size when being used for repairing large area of tissue defect is solved. Theoretically, any size of SIS material can be obtained by the compound SIS tissue repair material prepared by the method; in addition, the layers have good binding force, so that the defects of bidirectional isomerism of the SIS material are overcome in mechanics, and the obtained product can be used in large size tissue defect repairing.
Description
Technical field
The present invention relates to field of tissue engineering technology, specifically, relate to a kind of composite S IS tissue renovation material and preparation method thereof.
Background technology
Submucous layer of small intestine (Small Intestinal Submucosa is called for short SIS) is that a kind of degradable natural biological is learned material, and it has outstanding biological superiority and wide application prospect aspect tissue repair and regeneration.SIS class tissue repair and support product have been realized industrialization production abroad.Also there is at home a lot of correlational studyes.
Current, the various synthetic materials of the main application of repair tissue damage clinically, and allosome and autologous tissue's material.Synthetic material only can not be realized tissue reconstruction as support, and a series of side reaction easily occurs, and forms cicatrix.Although the repair tissue damage effectively of autologous and allosome material, its source is very limited, and autologous material also will cause patient's secondary insult, and it is applied and is all subject to great restriction.
SIS is that raw material sources are extensive take trees-Osima jacoti, Osima excavata as raw material, by biologically-derivedization treatment technology, and the novel regeneration and restoration material of development.This material has outstanding biological superiority and clinical value widely, is mainly used in the multiple soft tissue defects such as reconstruction hernia, cerebral dura mater damage, hypospadias, urethral stricture, spongy body are damaged, anal fistula, bronchopleural fistula.
The technology of preparing of SIS consists essentially of except virus, defat, de-cell, de-sludging, the dry and several steps of sterilizing.The SIS material of preparing through said process is that one can be regenerated by induced tissue, the thin-film material of reduced immunogenicity.Some researchs are prepared into the SIS after chemical treatment the material of bilayer or multilamellar by different combinations or bonding mode, simultaneously in order to obtain abundanter function, the SIS material of multilamellar is often prepared to the material of the multilamellar opposite sex.
As by SIS material multiple-level stack own, to make resulting materials there is better planar stretch performance than the SIS of monolayer, or better tensile property.Also have research that SIS and other de-cell tissue are combined into multilayer material, described other de-cellular material comprise, the tissues such as the abdominal cavity fascia of animal origin, pericardium, dermal tissue, submucous layer of bladder by similarly except virus, defat, take off the de-cell material that cell etc. goes Process of Antigen to obtain.
Also have by the SIS of different layers or from the de-cell tissues of other of SIS combination bioactive ingredients of compound different biological active components (as somatomedin) or variable concentrations respectively, make material reach the object of different performance.As by the above-mentioned multilamellar SIS mentioning or other de-cellular material of being combined with SIS, by the SIS of different layers or from the compound different somatomedin of other de-cell tissues of SIS combination, make each layer of prepared material have the performance of different induced tissue regeneration, realize more complicated tissue repair function; Or in other de-cellular material of being combined by multilamellar SIS or with SIS, the Material cladding variable concentrations bioactive ingredients of different layers, the activity that the induced tissue of each layer of the material of preparation is regenerated has the character of successively going forward one by one or weakening.For example, the method for transmitting stage by stage of disclosed graft materials and biological active component in application number 200880127971.5; The artificial cerebral dura mater that has dura mater and arachnoidea function concurrently is disclosed in application number 03239494.2.
But, because SIS derives from pig small intestine, general pig small intestine length is about 12-20m, but diameter generally can not exceed 4cm, therefore in the time repairing the larger tissue defect of area with SIS, the application of SIS is limited to its size, even the flaky material width that SIS is obtained along its axial cutting can not exceed 13cm yet.In addition, because pig small intestine are different with the mechanical property of hoop vertically, the SIS material of monolayer is good compared with the mechanical property of hoop along the axial mechanical property of original pig small intestine.As tissue renovation material, facing to the mechanical environment of inside of human body complexity, the restriction that the material of mechanics anisotropic may also can be applied, especially, in the time that reparation is subject to the abdominal cavity fascia of tension force impact, just more easily goes wrong after implant into body.
Be at present to add cross-linking agent in order to strengthen the active force usual way of SIS interlayer, the adding to differ of cross-linking agent be formed with surely the interlaminar action of effect, increased the biological safety risk of product simultaneously.
Summary of the invention
The object of this invention is to provide a kind of novel composite S IS tissue renovation material and preparation method thereof.
The present invention is by stacking according to special ply sequence by SIS, and clamp and pressurize with upper lower plate with holes, then dry, obtain a kind of large-sized, each layer compact, the SIS lamellar structure repair materials of the two-way same sex on mechanics, solves SIS and is limited in the time repairing the larger tissue defect of area the problem of its size.
In order to realize the object of the invention, a kind of composite S IS tissue renovation material of the present invention, its preparation method comprises the following steps:
1) get submucous layer of small intestine, through sterilization, machinery divests, SIS thin film is prepared in defat, de-cell and de-sludging, SIS axially cut open along intestinal tube, and along hoop, SIS thin film is cut into the rectangle SIS unit of arbitrary dimension, retains the hoop natural width of intestinal tube;
2) getting a flat board is laid on table top, first SIS unit is laid on flat board, scrape bubble removing, by second SIS unit and the overlapping placement of first SIS cell mesh, and along the hoop direction certain distance that staggers, guarantee the axially parallel of two SIS unit, the lap width of two SIS unit is greater than 1cm, the 3rd SIS unit is along hoop direction and second overlapping placement of SIS cell mesh before, make equally the axially parallel of two SIS unit, the lap width of two SIS unit is greater than 1cm, repeat successively, until SIS lays to required area, must lay a layer I,
3) separately get SIS unit and overlap laying along laying a layer I edge, guarantee long limit, this SIS unit and lay a layer I coincident, and this SIS unit shaft to the SIS unit shaft of laying layer I to vertical laying; Then lay to required area according to the mode of laying layer I, must lay layer II;
4) repeat as required above-mentioned steps 2) and 3) lay to desired thickness;
5) separately get a flat board and cover on the above-mentioned multilamellar SIS material laying and clamp, pressurization, and be dried, sterilizing, obtains composite S IS tissue renovation material finished product.
Aforesaid method, step 1) is according to " de-cell is processed the experimentation on small intestinal submucosa cell residue and growth factor content impact " Chen Wei, Li Cihui, wushu, Xie Huiqi, Luo Jingcong, (2010) Chinese Reconstructive surgery magazine, v.24 (01): the method for describing in 94-99, by drawing materials, sterilization, machinery divests, defat, de-cell, SIS thin film is prepared in de-sludging, and SIS is axially cut open along intestinal tube, and along hoop, SIS is cut into the rectangle of arbitrary dimension, retain the hoop natural width of intestinal tube.
Aforesaid method, step 2) and 5) in the flat board that uses made by medical macromolecular materials or medical alloy material.Described medical macromolecular materials or medical alloy material include but not limited to rustless steel, titanium alloy, polypropylene, politef, PVC etc.
Aforesaid method, is also included between any two SIS unit or two steps of laying interlayer interpolation binding agent.Wherein, described binding agent includes but not limited to gelatin solution (concentration is 0.01%～15%), a-cyanoacrylate compounds (molecular weight is 114-200 dalton) etc.The gelatin solution that preferably working concentration is 0.01%～15% is cross-linked, and gelatin solution is because biological safety is better, and has very strong bonding force after dry, is often used to the bonding of biomaterial.A-cyanoacrylate compounds is a kind of binding agent of meeting water cure, and its multiple derivant has started for the sealing to wound, and in some specific operation, successfully replaces the use of operation suture thread.Between two-layer SIS or two lay interlayer appropriate above-mentioned binding agents that add and can strengthen the interaction between multilamellar SIS.
Aforesaid method, dry lyophilizing or the heated drying of comprising described in step 5).
Lyophilizing operation is specially: in-10～-80 ℃, more than pre-lyophilizing 2h, then regulate the preferred 5<0Pa of vacuum pressure <300Pa(), more than lyophilizing 4h.
Heated drying operation is specially: in 50～75 ℃ (preferably 60～65 ℃, more preferably 62 ℃) heating 8min～24h, be then placed in 1～20 ℃ of quenching.Heating-up temperature needs enough high, just can make each layer of adhesion, but had better not be higher than collagen protein irreversible denaturation thermal transition temperature.Be 8min～24h heat time heating time, mainly according to the type of processing material, and thickness, humidity, heating-up temperature is adjusted.Mode of heating comprises the modes such as heating in water bath, irradiated heat, electric boiling plate.After having heated, immediately SIS plied timber is placed in to cryogenic conditions, temperature is controlled at 1～20 ℃ (preferably 1～10 ℃, more preferably 4 ℃).The object of quenching is to stop heating process, and sets up effective connection of SIS interlayer.Generally can adopt water-bath or gas bath to quench, water-bath quenching effect is relatively better.
Dried product is cut to required size and puts into packing, by ethylene oxide sterilizing or irradiation sterilization.
The present invention also provides the composite S IS tissue renovation material that adopts said method to prepare.
Adopt the composite S IS tissue renovation material prepared of the inventive method, can obtain in theory the SIS material of arbitrary dimension size.Each interlayer has good adhesion simultaneously, has made up the two heterotropic shortcomings of SIS material itself from mechanics.Products obtained therefrom can be applicable in the Repair of tissue defect of large-size.Its advantage is embodied in:
(1) stack manner partially overlapping while laying can extend SIS material in some directions.
(2) clamping force of upper lower plate can make SIS in dry volume contraction process, produce physics packing interaction between layers.
(3) mode of intersection laying has solved the two heterotropic shortcomings of SIS.
(4) between SIS or lay interlayer adhesive add the use that is different from material cross-linking agent, binding agent adds and has guaranteed that the bonding force producing all acts between SIS or lays interlayer laying when stacking, and the impurity of not only introducing that adds of cross-linking agent may exert an influence to the biological safety of material, and the effect of cross-linking agent is intermolecular effect, at cross-linking agent crosslinked two when intermolecular, cannot guarantee that crosslinked molecule comes from respectively between SIS or lays interlayer, major part comes from same SIS thin film inside on the contrary, the flexility of SIS is lost in the interior excessive crosslinked meeting of molecule of SIS thin film simultaneously to a certain extent, and then affect the mechanical performance of final products.
Accompanying drawing explanation
Figure 1 shows that the embodiment of the present invention 1 and 2 Small Intestine tissues are axial, hoop.
Fig. 2 is that in the embodiment of the present invention 1 and 2, the interior SIS of each laying layer lays pattern.
Fig. 3 is that in the embodiment of the present invention 1 and 2, adjacent laying interlayer is laid pattern.
The specific embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that in embodiment, technological means used is well known to those skilled in the art, the raw materials used commercial goods that is.
Embodiment 1 composite S IS tissue renovation material and preparation thereof
According to the method for describing in " de-cell is processed the experimentation on small intestinal submucosa cell residue and growth factor content impact ", by drawing materials, sterilize, machinery divests, SIS thin film is prepared in defat, de-cell, de-sludging, SIS is axially cut open along intestinal tube, and the rectangle that is 20cm by SIS cutting growth limit along hoop, the hoop natural width of reservation intestinal tube.Getting a stainless steel flat plate is laid on table top, to get first SIS is laid on flat board, scrape bubble removing, the placement that afterwards second SIS and first SIS partly overlapped, and to the hoop direction certain distance that staggers, the axially parallel of two SIS materials, the lap width of two SIS is 2cm, repeat successively, until SIS lays 25cm along hoop direction, laying area is that 20cm × 25cm forms ground floor laying layer.
Get again SIS and overlap laying along a ground floor laying layer edge, guarantee the long limit of this SIS and a ground floor laying layer coincident, and the axial axial vertical laying of SIS of laying layer with ground floor of this SIS.Lay the mode repetitive operation of layer until SIS laying area obtains required size according to ground floor afterwards, the laying area entirety of the SIS of gained forms the second layer and lays layer.
Repetitive operation is laid 4 layers altogether.Get another flat board and cover the upper clamping of the multilamellar SIS laying before, and pressurization.Fixture is placed in air dry oven, and temperature general control, at 62 ℃, is the water quench cooling of 4 ℃ by temperature after 6 hours.
Dried product is cut to 15cm × 15cm size and puts into packing, then be placed in ethylene oxide sterilizing cabinet, the oxirane that passes into certain concentration under certain humiture and pressure condition kept after a period of time, take out product and be placed in aeration-drying place, product is resolved, and product can use through being up to the standards.
Products obtained therefrom along long to wide to tensile force p value >0.05, average is 70N ± 5N.
Embodiment 2 composite S IS tissue renovation material and preparations thereof
According to the method for describing in " de-cell is processed the experimentation on small intestinal submucosa cell residue and growth factor content impact ", by drawing materials, sterilize, machinery divests, SIS thin film is prepared in defat, de-cell, de-sludging, SIS is axially cut open along intestinal tube, and the rectangle that is 15cm by SIS cutting growth limit along hoop, the hoop natural width of reservation intestinal tube.Getting a stainless steel flat plate is laid on table top, to get first SIS is laid on flat board, scrape bubble removing, the placement that afterwards second SIS and first SIS partly overlapped, and to the hoop direction certain distance that staggers, the axially parallel of two SIS materials, the lap width of two SIS is 1cm, repeat successively, until SIS lays 18cm along hoop direction, laying area is that 15cm × 18cm forms ground floor laying layer.
Get again and close SIS and lay a layer edge along ground floor and overlap laying, guarantee that the long limit of this SIS and ground floor lay a layer coincident, and this SIS axial vertical laying of SIS of axially laying layer with ground floor.Lay the mode repetitive operation of layer until SIS laying area obtains required size according to ground floor afterwards, the laying area entirety of the SIS of gained forms the second layer and lays layer.
Repetitive operation is laid 3 layers altogether.Get another flat board and cover the upper clamping of the multilamellar SIS laying before, and pressurization.Fixture is placed in profound hypothermia refrigerator, 80 ℃ of pre-cooling 2h, then to regulate vacuum pressure be dry 6h in 150Pa environment.
Dried product is cut to 6cm × 13cm size and puts into packing, irradiation sterilization.
Products obtained therefrom along long to wide to tensile force p value >0.05, average is 55N ± 5N.
Figure 1 shows that small intestine is axial, hoop.
Fig. 2 is that in each laying layer, SIS lays pattern.
Fig. 3 is that adjacent laying interlayer is laid pattern.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. the preparation method of composite S IS tissue renovation material, is characterized in that, comprises the following steps:
1) get submucous layer of small intestine, through sterilization, machinery divests, SIS thin film is prepared in defat, de-cell and de-sludging, SIS axially cut open along intestinal tube, and along hoop, SIS thin film is cut into the rectangle SIS unit of arbitrary dimension, retains the hoop natural width of intestinal tube;
2) getting a flat board is laid on table top, first SIS unit is laid on flat board, scrape bubble removing, by second SIS unit and the overlapping placement of first SIS cell mesh, and along the hoop direction certain distance that staggers, guarantee the axially parallel of two SIS unit, the lap width of two SIS unit is greater than 1cm, the 3rd SIS unit is along hoop direction and second overlapping placement of SIS cell mesh before, make equally the axially parallel of two SIS unit, the lap width of two SIS unit is greater than 1cm, repeat successively, until SIS lays to required area, must lay a layer I,
3) separately get SIS unit and overlap laying along laying a layer I edge, guarantee long limit, this SIS unit and lay a layer I coincident, and this SIS unit shaft to the SIS unit shaft of laying layer I to vertical laying; Then lay to required area according to the mode of laying layer I, must lay layer II;
4) repeat as required above-mentioned steps 2) and 3) lay to desired thickness;
5) separately get a flat board and cover on the above-mentioned multilamellar SIS material laying and clamp, pressurization, and be dried, sterilizing, obtains composite S IS tissue renovation material finished product.
2. method according to claim 1, is characterized in that step 2) and 5) in use flat board made by medical macromolecular materials or medical alloy material.
3. method according to claim 2, is characterized in that, described medical macromolecular materials or medical alloy material include but not limited to rustless steel, titanium alloy, polypropylene, politef, PVC.
4. method according to claim 1, is characterized in that, is also included between any two SIS unit or two steps of laying interlayer interpolation binding agent;
Wherein, described binding agent includes but not limited to that concentration is that 0.01%～15% gelatin solution, molecular weight are the daltonian a-cyanoacrylate compounds of 114-200.
5. method according to claim 1, is characterized in that, dry lyophilizing or the heated drying of comprising described in step 5).
6. method according to claim 5, is characterized in that, lyophilizing operation is specially: in-10～-80 ℃, more than pre-lyophilizing 2h, then regulate vacuum pressure <300Pa, more than lyophilizing 4h.
7. method according to claim 5, is characterized in that, heated drying operation is specially: in 50～75 ℃ of heating 8min～24h, be then placed in 1～20 ℃ of quenching.
8. method according to claim 7, is characterized in that, quenches and adopts water-bath or gas bath to quench.
9. method according to claim 1, is characterized in that, in step 5), sterilizing adopts ethylene oxide sterilizing or irradiation sterilization.
10. the composite S IS tissue renovation material of preparing according to method described in claim 1-9 any one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410035832.7A CN103877619B (en) | 2014-01-24 | 2014-01-24 | Composite S IS tissue renovation material and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410035832.7A CN103877619B (en) | 2014-01-24 | 2014-01-24 | Composite S IS tissue renovation material and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103877619A true CN103877619A (en) | 2014-06-25 |
| CN103877619B CN103877619B (en) | 2015-10-07 |
Family
ID=50946953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410035832.7A Active CN103877619B (en) | 2014-01-24 | 2014-01-24 | Composite S IS tissue renovation material and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103877619B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105749353A (en) * | 2016-02-22 | 2016-07-13 | 江苏期佰医疗技术有限公司 | Preparation method of esophagus restoration material by SIS (small intestinal submucosa) tissue |
| CN106039405A (en) * | 2015-04-08 | 2016-10-26 | 上海宏创医疗科技有限公司 | Preparation method for extracellular matrix (ECM) composite biological patch |
| CN106039404A (en) * | 2015-04-08 | 2016-10-26 | 上海宏创医疗科技有限公司 | Preparation method for extracellular matrix (ECM) composite biological patch |
| CN106178143A (en) * | 2016-08-30 | 2016-12-07 | 常州市康蒂娜医疗科技有限公司 | A kind of anti biological sticking patch and preparation method |
| CN107050520A (en) * | 2017-03-03 | 2017-08-18 | 北京博辉瑞进生物科技有限公司 | Compound bio sticking patch and preparation method thereof |
| CN107233624A (en) * | 2017-06-16 | 2017-10-10 | 卓阮医疗科技（苏州）有限公司 | A kind of splicing biomaterial of two-way mechanics uniform intensity and preparation method thereof |
| CN107320774A (en) * | 2017-06-16 | 2017-11-07 | 卓阮医疗科技（苏州）有限公司 | One kind splicing biomaterial and preparation method thereof |
| CN108472410A (en) * | 2015-11-17 | 2018-08-31 | 柏林工业大学 | Method for producing skin equivalent and its purposes transplanted for testing in vitro and in vivo |
| CN108837184A (en) * | 2017-07-12 | 2018-11-20 | 上海白衣缘生物工程有限公司 | A kind of composite membrane and preparation method thereof for Guided Bone Regeneration |
| CN112641999A (en) * | 2020-12-04 | 2021-04-13 | 陕西佰傲再生医学有限公司 | Film paving equipment and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996031226A1 (en) * | 1995-04-07 | 1996-10-10 | Purdue Research Foundation | Large area submucosal graft constructs and method for making the same |
| CN203220462U (en) * | 2013-04-07 | 2013-10-02 | 陕西佰傲再生医学有限公司 | Perforated biological patch |
-
2014
- 2014-01-24 CN CN201410035832.7A patent/CN103877619B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996031226A1 (en) * | 1995-04-07 | 1996-10-10 | Purdue Research Foundation | Large area submucosal graft constructs and method for making the same |
| CN203220462U (en) * | 2013-04-07 | 2013-10-02 | 陕西佰傲再生医学有限公司 | Perforated biological patch |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106039405A (en) * | 2015-04-08 | 2016-10-26 | 上海宏创医疗科技有限公司 | Preparation method for extracellular matrix (ECM) composite biological patch |
| CN106039404A (en) * | 2015-04-08 | 2016-10-26 | 上海宏创医疗科技有限公司 | Preparation method for extracellular matrix (ECM) composite biological patch |
| CN108472410A (en) * | 2015-11-17 | 2018-08-31 | 柏林工业大学 | Method for producing skin equivalent and its purposes transplanted for testing in vitro and in vivo |
| CN105749353A (en) * | 2016-02-22 | 2016-07-13 | 江苏期佰医疗技术有限公司 | Preparation method of esophagus restoration material by SIS (small intestinal submucosa) tissue |
| CN106178143B (en) * | 2016-08-30 | 2019-03-29 | 常州市康蒂娜医疗科技有限公司 | One kind prevents adhesion biological sticking patch and preparation method |
| CN106178143A (en) * | 2016-08-30 | 2016-12-07 | 常州市康蒂娜医疗科技有限公司 | A kind of anti biological sticking patch and preparation method |
| CN107050520A (en) * | 2017-03-03 | 2017-08-18 | 北京博辉瑞进生物科技有限公司 | Compound bio sticking patch and preparation method thereof |
| CN107050520B (en) * | 2017-03-03 | 2020-05-05 | 北京博辉瑞进生物科技有限公司 | Composite biological patch and preparation method thereof |
| CN107233624A (en) * | 2017-06-16 | 2017-10-10 | 卓阮医疗科技（苏州）有限公司 | A kind of splicing biomaterial of two-way mechanics uniform intensity and preparation method thereof |
| CN107320774A (en) * | 2017-06-16 | 2017-11-07 | 卓阮医疗科技（苏州）有限公司 | One kind splicing biomaterial and preparation method thereof |
| CN107320774B (en) * | 2017-06-16 | 2020-04-07 | 卓阮医疗科技（苏州）有限公司 | Spliced biological material and preparation method thereof |
| CN108837184A (en) * | 2017-07-12 | 2018-11-20 | 上海白衣缘生物工程有限公司 | A kind of composite membrane and preparation method thereof for Guided Bone Regeneration |
| CN112641999A (en) * | 2020-12-04 | 2021-04-13 | 陕西佰傲再生医学有限公司 | Film paving equipment and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103877619B (en) | 2015-10-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103877619B (en) | Composite S IS tissue renovation material and preparation method thereof | |
| US11058529B2 (en) | Composite soft tissue repair material stable in repair area | |
| US8658196B2 (en) | Graft materials and methods for staged delivery of bioactive components | |
| CA2703103C (en) | Use of a regenerative biofunctional collagen biomatrix for treating visceral or parietal defects | |
| CN103251987B (en) | Acellular biological patch, preparation method and apparatus thereof | |
| CN107050519B (en) | Preparation method of multilayer absorbable biological membrane | |
| US20120158134A1 (en) | Mastopexy and Breast Reconstruction Prostheses and Method | |
| US9480771B2 (en) | Hemostatic device | |
| CN103418031A (en) | Absorbable endocranium healing patch and preparation method thereof | |
| CN106310373A (en) | Biological repair membrane and preparation method thereof | |
| Miyamoto et al. | The effects of sheet-type absorbable topical collagen hemostat used to prevent pulmonary fistula after lung surgery | |
| CN108114320A (en) | Tissue repair sticking patch, main body and preparation method | |
| US20220184275A1 (en) | Multi-layer collagen-based membrane | |
| Ale-Ebrahim et al. | The effect of cold argon plasma in atmospheric pressure on increasing blood coagulation speed and full-thickness cutaneous wound healing in rats | |
| US11826490B1 (en) | Extracellular matrix sheet devices with improved mechanical properties and method of making | |
| CN115382019A (en) | Composite material of silk fibroin and acellular matrix and preparation method thereof | |
| WO2022182646A1 (en) | Collagen matrix and n-hydroxylsuccinimide functionalized polyethylene glycol staple line reinforcement | |
| CN116322809A (en) | Adhesive for surgical suture reinforcement | |
| CN107233624A (en) | A kind of splicing biomaterial of two-way mechanics uniform intensity and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 100085 C, block, 3rd Street, Beijing, Haidian District, China C701 Patentee after: Beijing Daqing biotechnology Limited by Share Ltd Address before: 100085 Beijing city Haidian District Third Street No. 9 C-701 Wah Building Patentee before: Beijing Daqing Biotechnology Co., Ltd. |
|
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Dai Jinming Inventor after: Niu Rui Inventor after: Li Zhifeng Inventor after: Zhang Haiyan Inventor after: Chen Defu Inventor before: Niu Rui Inventor before: Li Zhifeng Inventor before: Zhang Haiyan Inventor before: Chen Defu |