CN106018053A - Micro red cell collecting device for detecting glycated hemoglobin, and collecting and detecting method - Google Patents
Micro red cell collecting device for detecting glycated hemoglobin, and collecting and detecting method Download PDFInfo
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- CN106018053A CN106018053A CN201610515057.4A CN201610515057A CN106018053A CN 106018053 A CN106018053 A CN 106018053A CN 201610515057 A CN201610515057 A CN 201610515057A CN 106018053 A CN106018053 A CN 106018053A
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- blood
- sucting pad
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 102000017011 Glycated Hemoglobin A Human genes 0.000 title abstract description 6
- 108091005995 glycated hemoglobin Proteins 0.000 title abstract 5
- 239000008280 blood Substances 0.000 claims abstract description 151
- 210000004369 blood Anatomy 0.000 claims abstract description 149
- 238000001514 detection method Methods 0.000 claims abstract description 44
- 102000001554 Hemoglobins Human genes 0.000 claims description 41
- 108010054147 Hemoglobins Proteins 0.000 claims description 41
- 239000012790 adhesive layer Substances 0.000 claims description 38
- 210000003743 erythrocyte Anatomy 0.000 claims description 30
- 239000000758 substrate Substances 0.000 claims description 26
- 239000000463 material Substances 0.000 claims description 21
- 238000010521 absorption reaction Methods 0.000 claims description 13
- 239000000853 adhesive Substances 0.000 claims description 7
- 230000001070 adhesive effect Effects 0.000 claims description 7
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- 230000002745 absorbent Effects 0.000 claims description 3
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- 229920000742 Cotton Polymers 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 12
- 238000000605 extraction Methods 0.000 abstract description 5
- 238000005070 sampling Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 29
- 238000005534 hematocrit Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000005457 optimization Methods 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
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- CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
- G01N2333/805—Haemoglobins; Myoglobins
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a micro red cell collecting device for detecting glycated hemoglobin. According to the device provided by the invention, a blood sucking pad, a porous bonding layer and other structures are arranged so that the extraction of packed red cells can be realized on one device. The device has the advantages of simple structure, convenience and quickness in detection and the like. The invention also provides a micro red cell collecting method. According to the method provided by the invention, the micro red cells can be directly collected from a blood sample by using the collecting device provided by the invention, so that the problem that the required sampling operation is complicated in the prior art is solved. Meanwhile, the invention also provides a glycated hemoglobin detecting method. According to the method provided by the invention, the packed cell cells are collected by using the device provided by the invention, and the glycated hemoglobin is directly detected further, so that the technical problem that the inconvenience in operation on detection of glycated hemoglobin is brought by joint use of multiple devices is solved.
Description
Technical field
The present invention relates to red blood cell collection device field, concrete, the present invention relates to a kind of HbAle
Protein Detection trace red blood cell collection device and acquisition testing.
Background technology
Blood is made up of blood plasma and hemocyte.In Clinical detection, general conventional blood specimen is blood
Slurry, but also have project to need packed red cells as detection specimen, such as biochemistry detection saccharifying blood
During Lactoferrin, it is necessary to using packed red cells as detection specimen.
Glycolated hemoglobin is the product that in blood of human body, endoerythrocytic hemoglobin is combined with blood glucose.
The general percentage ratio using glycolated hemoglobin to account for total hemoglobin clinically is as weighing HbAle egg
The most normal index of Bai Hanliang.Glycolated hemoglobin accounts for the percentage ratio of total hemoglobin and can stably may be used
That leans on reflects the detection average blood glucose levels of first 4 months, and this detection will not by the blood drawing time,
The most on an empty stomach, if use the factor interference such as insulin, it is that diabetes monitor " goldstandard " in the world.
The most common glycosylated hemoglobin detection kit (such as enzyme process, immunoturbidimetry etc.) is all
The hematocrit needing the whole blood sample using vacuum test tube to gather to be formed through centrifugal or natural subsidence is red
Cell is to picture and next by measuring the content of glycolated hemoglobin in packed red cells sample as detection
Calculate glycolated hemoglobin and account for the percentage ratio of total hemoglobin, such that it is able to avoid each sample independent
Carry out complex detection and operation that total hemoglobin detection is brought.Obtain the most quickly and easily
Packed red cells just becomes an important problem.
In the prior art, in order to obtain fixed amount packed red cells, and HbAle is carried out further
Percent protein detects, it usually needs blood collecting, separation packed red cells, absorption fixed body hematocrit
Long-pending erythrocyte these three step.Specifically, when existing glycolated hemoglobin percentage ratio detects, one
As first by vacuum test tube collect venous blood, or from human body tip extraction certain volume whole blood
In a container；Then, take to be centrifuged, the method such as natural subsidence, filter element filtering makes erythrocyte and blood
Slurry separates；Finally, the packed red cells re-using liquid-transfering device absorption fixed amount carries out HbAle egg
The detection of white percentage ratio.
But, the process CIMS of detection method of the prior art is complicated, and time-consumingly, cost is the most relatively
Height, blood sampling volume is bigger than normal.The centrifuge that such as centrifugal needs are special, needs to provide power supply for centrifuge；
And natural subsidence, need to expend the waiting time of a few hours；Meanwhile, the filter of the special material of needs is filtered
Film, and the erythrocyte being filtrated to get is not easily taken out and tests；Same, last solid pressure is long-pending red
The collection of cell is also required to use pipettor to shift.Whole flow process wastes time and energy, and is difficult to realize
Miniaturization portability.
Therefore, develop a kind of miniaturization packed red cells harvester and easily hematocrit red carefully
The acquisition method of born of the same parents, has the most just become the emphasis that present people study.
In view of this, the special proposition present invention.
Summary of the invention
The first object of the present invention is to provide a kind of trace for glycolated hemoglobin detection red carefully
Born of the same parents' harvester.In the present invention, directly draw and fix blood sample by arranging blood sucting pad, thus
Avoid the use of blood collection container；Meanwhile, in the present invention, by arranging porous adhesive layer, from
And blood sucting pad can be fixed, the most also do not interfere with blood sucting pad and carry out the absorption of blood sample.Enter one
Step, apparatus of the present invention are after drawing blood sample, by simply being removed by unnecessary blood sample, it becomes possible to
Realize the extraction of packed red cells and fix, such that it is able to it is red to utilize a device to be i.e. capable of hematocrit
The extraction of cell, and then avoid the many devices connection used in prior art for extracting packed red cells
The technical problem of miniaturization, facilitation cannot be realized with the device brought.Apparatus of the present invention have knot
Structure is simple, the advantage such as fast easy to detect.
Second object of the present invention is, it is provided that a kind of erythrocytic acquisition method of trace, the present invention
In method, the application of the invention device is harvester, such that it is able to directly from Blood specimen collection
Trace erythrocyte, and then solve in prior art packed red cells gatherer process owing to using multiple dresses
Put and the technical problem of complex operation that multi-step is brought operation.The inventive method has operation letter
Just, the advantage such as quick.
Third object of the present invention is to provide a kind of glycolated hemoglobin detection method.Side of the present invention
In method, the application of the invention device gathers packed red cells, and the most directly detects HbAle
Albumen, thus owing to using the associating of many devices during solving the detection of prior art glycolated hemoglobin
The unhandy technical problem used and bring.The inventive method has that operational approach is convenient, result
The advantage such as accurately.
In order to realize above-mentioned purpose of the present invention, the application spy adopts the following technical scheme that
A kind of trace red blood cell collection device for glycolated hemoglobin detection, described device includes solid
Part, blood sucting pad and porous adhesive layer；Described blood sucting pad is arranged on one end of described firmware；Described many
Hole adhesive layer is by wrapping up described blood sucting pad and being provided with the end of blood sucting pad by described blood sucting pad admittedly
On described firmware.
In the present invention, by arranging blood sucting pad and covering its porous adhesive layer, such that it is able to suction
Blood pad carries out position to be fixed, and directly carries out drawing of blood sample and fixing of further packed red cells,
Thus avoid packed red cells in prior art and extract multiple instruments, the complicated behaviour of multi-step used
Make.Apparatus of the present invention have a simple structure, the advantage such as fast easy to detect.
Optionally, in the present invention, a length of 3-10cm of described device.
Optionally, in the present invention, the width of described device is 1.5-3.5mm；Preferably, in the present invention,
The width of described device is 2mm.
Optionally, in the present invention, the length of described blood sucting pad is less than the length of described substrate, it is possible to
According to drawing blood volume and the difference of the most fixing packed red cells amount, its length is adjusted.
Optionally, in the present invention, can according to draw and to fix packed red cells amount difference and
The aperture of porous adhesive layer it described is adjusted.
Optionally, in the present invention, described firmware is a bar shaped substrate, and described blood sucting pad is arranged on described
On one side of bar shaped substrate, and extend to its end.Further, described porous adhesive layer
One end is fixed on the side of described bar shaped substrate, and the other end is fixed on and is provided with the side of blood sucting pad
On relative side；And the width of described porous adhesive layer is identical with the width of described bar shaped substrate.
In the present invention, by described board structure and described porous adhesive layer position, planform
Adjust further and optimize so that apparatus of the present invention are in use, described in suck blood
Draw blood sample at side that pad can never be wrapped by and porous adhesive layer hole, and then enter one
Step improves absorption and the fixed efficiency of sample.
Optionally, in the present invention, described device still further comprises blood removal device；Wherein, institute
State blood removal device to be arranged on described firmware, and be slidably connected with described firmware.
In the present invention, by arranging blood removal device further, such that it is able to enterprising at same device
One step realizes the removal of loose excess blood, and then achieves the integration that packed red cells extracts
Device operating procedure so that detect more convenient.
Optionally, in the present invention, described blood removal device is connected to described firmware by clamp structure.
Optionally, in the present invention, described blood removal device is porous or membranaceous adsorbing material.
In the present invention, by use porous or film material as blood removal device, such that it is able to will
The blood do not fixed by blood sucting pad fully draws removing, thus improves the accuracy of detection.
Optionally, in the present invention, described porous or film material be hydroscopic high-molecular resin, Cotton Gossypii with
And the mixing material of one or more in absorbent paper, sponge.
In the present invention, by adjusting further and optimizing blood removal device material used, use
The hydroscopic high-molecular resins that performance is more excellent etc. are as blood removal device material, and utilize blood to go
Except different caused the two of device materials and blood sucting pad material performance of sucking blood is different, such that it is able to enter
Unnecessary sample is removed by one step, and then can improve efficiency and the accuracy of detection further.
Optionally, in the present invention, described firmware is PET material firmware.
In the present invention, by the selection of baseplate material and optimization, use and there is excellent physical mechanical
The PET of performance is as baseplate material, thus improves the structural stability of apparatus of the present invention.
Optionally, in the present invention, described blood sucting pad is glass fibre blood sucting pad.
In the present invention, by the selection of blood sucting pad material and optimization, and select that there is good hematocrit
The glass fibre of erythrocyte crystallized ability is as blood sucting pad material, thus improves apparatus of the present invention detection
Accuracy.
Optionally, in the present invention, described porous adhesive layer is porous adhesive waterproof tape.
In the present invention, by the selection of porous adhesion-layer materials and optimization, and select to have good viscous
Conjunction ability simultaneously will not the porous adhesive waterproof tape of draw blood as adhesive layer, such that it is able to improving this
While invention apparatus structure stability, also do not interfere with the accuracy of apparatus of the present invention detection.
Optionally, the present invention is for the preparation of the trace red blood cell collection device of glycolated hemoglobin detection
Method is as follows: stick 4mm in the bottom of the rectangle PET sheet backing of a width about 3 ￣ 10cm
Wide fiberglass packing, then with mackintosh with holes by monoblock rectangle PET sheet with post glass
One end of fiber mat wraps, and ensures that this one end wrapped up is seamless, finally entire plate is cut into
The bar that 2mm is wide, and obtain being about 3 ￣ 10cm, apparatus of the present invention of wide about 2mm.Further,
Described preparation method still further comprises passes through gripper mechanism grips in described base by blood removal device
The step at the middle part of plate.Meanwhile, a kind of method that present invention also offers trace red blood cell collection, institute
State and method uses device of the present invention.Further, described method specifically includes following steps:
The structure division being glued with blood sucting pad of heretofore described red blood cell collection device is placed in blood sample
Carry out sample absorption in Ben, and by extruding absorption, the blood sample do not fixed by described blood sucting pad is removed
Go, and obtain gathered trace erythrocyte.
In the inventive method, the application of the invention device is red blood cell collection device, such that it is able to directly
Connect from Blood specimen collection erythrocyte, and then solve in prior art packed red cells gatherer process many
The technical problem of the troublesome operation that device uses and brings.The inventive method has easy and simple to handle, quick
Etc. advantage.
Optionally, the acquisition method that method is packed red cells of trace red blood cell collection of the present invention；
The trace erythrocyte gathered is packed red cells.
Further, the method that present invention also offers the detection of a kind of glycolated hemoglobin, described method
Middle use device of the present invention.Further, described method comprises the steps: institute of the present invention
State the structure division being glued with described blood sucting pad of red blood cell collection device to be placed in blood sample and carry out
Sample is drawn, and is removed by the blood sample do not fixed by blood sucting pad by extruding absorption, then should
Structure division is placed in hemoglobin detectable liquid, carries out glycolated hemoglobin detection.
In the inventive method, the application of the invention device gathers packed red cells, and the most direct
Detection glycolated hemoglobin, thus owing to adopting during solving the detection of prior art glycolated hemoglobin
The unhandy technical problem being used in combination with many devices and bring.The inventive method has operation side
The advantages such as method is convenient, result is accurate.
Compared with prior art, the invention have the benefit that
(1) apparatus of the present invention simple structure, it is possible to realize in the same apparatus blood sample absorption and
The extraction of packed red cells is fixed, thus has use, the advantage such as fast easy to detect.
(2) in the present invention, by the selection of part structure material each in device and optimization, thus
Apparatus of the present invention are made to have good structural stability, even if long-time placement also will not deform upon
Or structure comes off.
(3), in the inventive method, the application of the invention device is packed red cells harvester,
Thus have easy to operate, the advantage such as easy to use.
Accompanying drawing explanation
In order to be illustrated more clearly that the specific embodiment of the invention or technical scheme of the prior art, under
The accompanying drawing used required in detailed description of the invention or description of the prior art will be briefly described by face,
It should be evident that the accompanying drawing in describing below is some embodiments of the present invention, general for this area
From the point of view of logical technical staff, on the premise of not paying creative work, it is also possible to obtain according to these accompanying drawings
Obtain other accompanying drawing.
Fig. 1 is the trace red blood cell collection device for glycolated hemoglobin detection provided by the present invention
Structural representation, 101-firmware, 102-porous adhesive layer, 103-blood sucting pad；
Fig. 2 is the trace red blood cell collection device for glycolated hemoglobin detection provided by the present invention
Structural representation, 101-firmware, 102-blood removal device, 103-porous adhesive layer, 104-sucks blood
Pad.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearer, below by the skill to the present invention
Art scheme carries out clear, complete description, and based on the detailed description of the invention in the present invention, this area is general
Other embodiments all that logical technical staff is obtained on the premise of not making creative work,
Broadly fall into the scope that the present invention is protected.
In describing the invention, it should be noted that term " " center ", " on ", D score, " left ",
Orientation or the position relationship of the instruction such as " right ", " vertically ", " level ", " interior ", " outward " are based on attached
Orientation shown in figure or position relationship, be for only for ease of description the present invention and simplify describe rather than
Instruction or the hint device of indication or element must have specific orientation, with specific azimuth configuration and
Operation, is therefore not considered as limiting the invention.Additionally, term " first ", " second ", "
Three " it is only used for describing purpose, and it is not intended that indicate or hint relative importance.
In describing the invention, it should be noted that unless otherwise clearly defined and limited, art
Language " is installed ", " being connected ", " connection " should be interpreted broadly, and connects for example, it may be fixing,
Can also be to removably connect, or be integrally connected；Can be to be mechanically connected, it is also possible to be electrical connection；
Can be to be joined directly together, it is also possible to be indirectly connected to by intermediary, can be two element internals
Connection.For the ordinary skill in the art, can understand that above-mentioned term is at this with concrete condition
Concrete meaning in invention.
Embodiment 1
Refer to Fig. 1, a kind of trace for glycolated hemoglobin detection provided by the present invention is red carefully
Born of the same parents' harvester, comprising: firmware 101, porous adhesive layer 102, blood sucting pad 103；
Wherein, described blood sucting pad 103 is arranged on one end of described firmware 101；Described porous adhesive layer
102 by wrapping up described blood sucting pad 103 and being provided with the end of blood sucting pad 103 by described blood sucting pad
103 are fixed on described firmware 101.
Embodiment 2
Refer to Fig. 1, a kind of trace for glycolated hemoglobin detection provided by the present invention is red carefully
Born of the same parents' harvester, comprising: firmware 101, porous adhesive layer 102, blood sucting pad 103；
Wherein, stating firmware 101 is a bar shaped substrate, and described blood sucting pad 103 is arranged on described bar shaped base
On one side of plate, and extend to its end；
Meanwhile, one end of described porous adhesive layer 102 is fixed on the side of described bar shaped substrate, separately
One end is fixed on the side relative with the side being provided with blood sucting pad 103；And described porous adhesive layer
The width of 102 is identical with the width of described bar shaped substrate.
Further, described firmware 101 is PET material firmware, and described porous adhesive layer 102 is
Porous adhesive waterproof tape, described blood sucting pad 103 is glass fibre blood sucting pad.
Embodiment 3
Refer to Fig. 1, a kind of trace for glycolated hemoglobin detection provided by the present invention is red carefully
Born of the same parents' harvester, comprising: firmware 101, porous adhesive layer 102, blood sucting pad 103；
Wherein, stating firmware 101 is a bar shaped substrate, and described blood sucting pad 103 is arranged on described bar shaped base
On one side of plate, and extend to its end；
Meanwhile, one end of described porous adhesive layer 102 is fixed on the side of described bar shaped substrate, separately
One end is fixed on the side relative with the side being provided with blood sucting pad 103；And described porous adhesive layer
The width of 102 is identical with the width of described bar shaped substrate.
Further, described firmware 101 is PET material firmware, and described porous adhesive layer 102 is
Porous adhesive waterproof tape, described blood sucting pad 103 is glass fibre blood sucting pad；
Meanwhile, a length of 3cm of described substrate 101, the width of described substrate 101 is 2mm.
Embodiment 4
Refer to Fig. 2, a kind of trace for glycolated hemoglobin detection provided by the present invention is red carefully
Born of the same parents' harvester, comprising: firmware 101, blood removal device 102, porous adhesive layer 103, inhales
Blood pad 104；
Wherein, described blood sucting pad 104 is arranged on one end of described firmware 101；Described porous adhesive layer
103 by wrapping up described blood sucting pad 104 and being provided with the end of blood sucting pad 104 by described blood sucting pad
104 are fixed on described firmware 101；Described blood removal device 102 is arranged on described firmware 101
On, and be slidably connected with described firmware 101.
Embodiment 5
Refer to Fig. 2, a kind of trace for glycolated hemoglobin detection provided by the present invention is red carefully
Born of the same parents' harvester, comprising: firmware 101, blood removal device 102, porous adhesive layer 103, inhales
Blood pad 104；
Wherein, stating firmware 101 is a bar shaped substrate, and described blood sucting pad 104 is arranged on described bar shaped base
On one side of plate, and extend to its end；One end of described porous adhesive layer 103 is fixed on institute
Stating on the side of bar shaped substrate, the other end is fixed on the side relative with the side being provided with blood sucting pad 104
On face；And the width of described porous adhesive layer 103 is identical with the width of described bar shaped substrate；
Meanwhile, described blood removal device 102 is arranged on described firmware 101, and with described firmware
101 are slidably connected.
Further, described firmware 101 is pet substrate, and described blood removal device 102 is sea
Continuous blood removal device, described porous adhesive layer 103 is porous adhesive waterproof tape, described blood sucting pad 104
For glass fibre blood sucting pad.
Embodiment 6
Refer to Fig. 2, a kind of trace for glycolated hemoglobin detection provided by the present invention is red carefully
Born of the same parents' harvester, comprising: firmware 101, blood removal device 102, porous adhesive layer 103, inhales
Blood pad 104；
Wherein, stating firmware 101 is a bar shaped substrate, and described blood sucting pad 104 is arranged on described bar shaped base
On one side of plate, and extend to its end；One end of described porous adhesive layer 103 is fixed on institute
Stating on the side of bar shaped substrate, the other end is fixed on the side relative with the side being provided with blood sucting pad 104
On face；And the width of described porous adhesive layer 103 is identical with the width of described bar shaped substrate；
Meanwhile, described blood removal device 102 is arranged on described firmware 101, and is tied by clamping
Structure is slidably connected with described firmware 101.
Further, described firmware 101 is pet substrate, and described blood removal device 102 is sea
Continuous blood removal device, described porous adhesive layer 103 is porous adhesive waterproof tape, described blood sucting pad 104
For glass fibre blood sucting pad；
Meanwhile, a length of 10cm of described substrate 101, the width of described substrate 101 is 2mm
Experimental example 1
By the trace red blood cell collection device being used for glycolated hemoglobin detection of the embodiment of the present invention 3
Structure division immersion blood sample with blood sucting pad 103 carries out blood absorption；Then, device is taken out,
Then use absorbent paper that blood sucting pad 103 is extruded, unnecessary blood sample is drawn and removes, i.e. obtain institute
Gather trace erythrocyte, namely packed red cells.
Then, device is soaked in hemoglobin detectable liquid with the structure division of blood sucting pad 103
In, thus packed red cells is discharged, and carry out further glycolated hemoglobin detection.
Experimental example 2
By the trace red blood cell collection device being used for glycolated hemoglobin detection of the embodiment of the present invention 6
Structure division immersion blood sample with blood sucting pad 104 carries out blood absorption；Then, device is taken out,
Then blood removal device 102 is moved to blood sucting pad 104 by clamping device, and to sucking blood
Pad 104 extrudes, and is removed by unnecessary blood sample, i.e. obtains gathered trace erythrocyte, namely press
Long-pending erythrocyte.
Then, device is soaked in hemoglobin detectable liquid with the structure division of blood sucting pad 104
In, thus packed red cells is discharged, and carry out further glycolated hemoglobin detection.
In the present invention, blood sample is effectively drawn by it by blood sucting pad, and utilizes blood to remove dress
Put and suck blood the different of ability from blood sucting pad and unnecessary blood sample is removed, and then it is red carefully to obtain hematocrit
Born of the same parents, and carry out glycolated hemoglobin detection.The present invention has that apparatus structure is simple, be prone to miniaturization,
Detection method simple operation, the advantages such as accuracy is good.
Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that and do not carrying on the back
May be made that in the case of the spirit and scope of the present invention many other change and amendment.Therefore,
This means all these changes including belonging in the scope of the invention in the following claims and repair
Change.
Claims (10)
1., for a trace red blood cell collection device for glycolated hemoglobin detection, its feature exists
In, described device includes firmware, blood sucting pad and porous adhesive layer；Described blood sucting pad is arranged on described
One end of firmware；Described porous adhesive layer is by wrapping up described blood sucting pad and being provided with the end of blood sucting pad
Described blood sucting pad is fixed on described firmware by portion.
Device the most according to claim 1, it is characterised in that described firmware is a bar shaped base
Plate, described blood sucting pad is arranged on a side of described bar shaped substrate, and extends to its end；
One end of described porous adhesive layer is fixed on the side of described bar shaped substrate, and the other end is fixed on
On the side relative with the side being provided with blood sucting pad；And the width of described porous adhesive layer and described bar
The width of shape substrate is identical.
Device the most according to claim 1, it is characterised in that described device wraps the most further
Include blood removal device；
Wherein, described blood removal device is arranged on described firmware, and is slidably connected with described firmware.
Device the most according to claim 3, it is characterised in that described blood removal device is
Porous or membranaceous adsorbing material.
Device the most according to claim 4, it is characterised in that described porous or membranaceous absorption
Material is the mixing of one or more in hydroscopic high-molecular resin, Cotton Gossypii, absorbent paper, sponge.
Device the most according to claim 1, it is characterised in that described firmware is PET
Material firmware.
Device the most according to claim 1, it is characterised in that described blood sucting pad is glass fibers
Dimension blood sucting pad.
Device the most according to claim 1, it is characterised in that described porous adhesive layer is many
Hole adhesive waterproof tape.
9. the method for a trace red blood cell collection, it is characterised in that described method includes walking as follows
Rapid: by the structure being glued with blood sucting pad of red blood cell collection device according to any one of claim 1-8
It is partially disposed in blood sample and carries out sample absorption, and drawn solid by described blood sucting pad by extruding
Fixed blood sample removes, and obtains gathered trace erythrocyte.
10. the method for a glycolated hemoglobin detection, it is characterised in that described method includes as follows
Step: red blood cell collection device according to any one of claim 1-8 is glued with described blood sucting pad
Structure division be placed in blood sample and carry out sample absorption, and drawn by extruding will be by blood sucting pad
Fixing blood sample removes, and is then placed in hemoglobin detectable liquid by this structure division, enters
Row glycolated hemoglobin detects.
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| CN201610515057.4A CN106018053B (en) | 2016-07-01 | 2016-07-01 | Glycosylated hemoglobin detection micro red blood cell collection device and acquisition testing method |
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| CN201610515057.4A CN106018053B (en) | 2016-07-01 | 2016-07-01 | Glycosylated hemoglobin detection micro red blood cell collection device and acquisition testing method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4397248A1 (en) * | 2023-01-06 | 2024-07-10 | Anbio Biotechnology Limited | Micro red blood cell collection device and method for collecting and detecting glycosylated hemoglobin |
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| CN106018053B (en) | 2019-04-30 |
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Denomination of invention: Micro red blood cell collection device and collection and detection method for glycosylated hemoglobin detection Effective date of registration: 20210208 Granted publication date: 20190430 Pledgee: Bank of China Limited by Share Ltd. Xiamen branch Pledgor: ANBIO (XIAMEN) PRODUCTS Inc. Registration number: Y2021980001126 |
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