CN210136235U - Whole blood sample detection device - Google Patents
Whole blood sample detection device Download PDFInfo
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- CN210136235U CN210136235U CN201920677335.5U CN201920677335U CN210136235U CN 210136235 U CN210136235 U CN 210136235U CN 201920677335 U CN201920677335 U CN 201920677335U CN 210136235 U CN210136235 U CN 210136235U
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Abstract
The utility model provides a whole blood sample detection device, including lower casing, lower casing includes base and support position, the height of base is not less than support position and the length of base is less than the length of lower casing to enclose into plasma collection chamber with support position jointly, support position is used for setting up red blood cell treatment layer, the base is used for placing the test paper strip, the test paper strip includes glass fiber membrane, cellulose nitrate membrane and the water absorption membrane that meets in proper order and sets up on the end card, the one end of glass fiber membrane is longer than the end card and introduces in the plasma collection chamber; the device cooperates the chromatography test paper strip, can save sample preliminary treatment step, and optimization operation is experienced, improves whole blood sample detection's CV value and sensitivity, and the testing result more is close serum, plasma detection level, provides more convenient, reliable product for clinical use.
Description
Technical Field
The utility model belongs to the field of medical equipment, a whole blood sample detection device is related to, especially relate to a one step of detection device of whole blood sample for chromatography kit.
Background
With the increasing demand of modern medicine for bedside rapid diagnostic reagents for detecting various markers, especially POCT, immunochromatography is used as the mainstream method of rapid detection and is applied to the detection of various markers. However, considering the influence of red blood cells on the chromatographic method, most products on the market mainly detect serum and plasma, and for a whole blood sample, a whole blood filter needs to be directly added to a sample pad or centrifuged or diluted.
CN206740772U discloses a N-terminal brain natriuretic peptide precursor immunochromatography quantification kit in human whole blood, its characterized in that the card shell cover on be provided with whole blood application of sample groove, whole blood application of sample groove bottom have into the sample hole, whole blood application of sample groove lateral wall on be equipped with a plurality of overflow sample holes, one side of whole blood application of sample groove be equipped with whole blood diluent and add the sample hole.
CN204177805U discloses a short-term test paper strip, this test paper strip compares and has increased the whole blood filter membrane in general test paper strip structure, and blood sample dropwise add to on the glass fiber, through whole blood filter membrane separation whole blood, red blood cell is combined on whole blood filter membrane in the blood, and serum chromatography reacts to the mark pad, improves reaction sensitivity. However, the patent has the risk that red blood cells block the filtering membrane and even directly wash the glass fiber membrane, so that the detection background is increased, and the heterogeneity caused by different samples is more obvious.
CN208104413U discloses a side blood filtering structure and a biochip, wherein the side blood filtering structure comprises a shell, a filtering membrane is arranged in the shell, a first cavity with an upward opening is arranged at the top of the shell, a second cavity is arranged in the shell, the first cavity and the second cavity are communicated through the filtering membrane, and the filtering membrane is vertical to a horizontal plane; still be equipped with the volume chamber in the shell, the volume chamber communicates with first cavity bottom, and the volume chamber passes through filtration membrane and communicates with the second cavity, and the volume in volume chamber is less than the volume of first cavity, and the volume in volume chamber is more than or equal to the total volume of treating the red blood cell in the filtered whole blood. The patent ensures that red blood cells are not easy to block a filter membrane, the blood filtration is sufficient, the blood filtration time is short, the efficiency is high, but the design of the vertical membrane surface is not suitable for most flat plate clamping groove type test instruments.
CN108226478A discloses a whole blood immunity detection device, including centrifuge and the micro-fluidic chip of installing on centrifuge, be provided with application of sample groove, test hole and channel on the micro-fluidic chip, application of sample groove and test hole pass through the channel intercommunication, are provided with nanometer gold particle and detection probe in the test hole, and the one end of test hole is provided with the parallel light subassembly, and the other end of test hole is provided with the spectral analysis subassembly. This device is relatively complex and not conducive to rapid detection and application.
CN108469521A discloses a whole blood full-quantitative detection immunochromatography device, which comprises an upper cover and a lower cover, wherein a plurality of anti-pressure nails are distributed on the inner side edge of the upper cover, a plurality of nail holes corresponding to the positions of the anti-pressure nails are distributed on the inner side edge of the lower cover, and each anti-pressure nail is embedded into the corresponding nail hole to cover the upper cover and the lower cover; a bayonet is arranged on the inner side of the lower cover, and a reagent strip is placed in an accommodating cavity formed by the nail holes and the bayonet after the lower cover is covered; the surface of the upper cover is provided with a sample adding window, a detection window adjacent to the sample adding window and a handheld part positioned at one end part; the periphery of the sample adding window is composed of smooth inclined planes which incline to the bottom and form a hollow quadrangle at the bottom, and the bottom of the sample adding window corresponds to the filtering gasket of the reagent strip; 4 prevent pressing the nail and distribute respectively in the upper cover border department that corresponds with 4 angles of application of sample window, 4 nail holes distribute respectively in the lower cover border department that corresponds with 4 prevent pressing the nail. This device suffers from the drawback that the blood sample is in direct contact with the glass fibre membrane.
CN208520872U discloses an animal plasma detection device, including the centrifuge bowl, the centrifuge bowl passes through the pipeline and is connected with detection device, the centrifuge bowl passes through the frame to be fixed on the workstation, centrifuge bowl bottom is connected with inlet means, the centrifuge bowl top is connected with drive arrangement, centrifuge bowl upper portion passes through the pipeline and is connected with detection device, detection device includes mixing cup, mixing cup upper end sets up the stopper, the cover body that the opening is downward is established to the cover on the pipeline, the cover body can be screwed on the stopper, the pipeline tip can stretch into in the mixing cup, detection device sets up on adjusting device, adjusting device includes the supporting shoe, set up the fixed slot that holds stopper and mixing cup with the axle center on the supporting shoe, the supporting shoe bottom surface is connected with the telescopic link, the supporting shoe can be under the effect of telescopic link translation from top to bottom, set up the translucent cover that surrounds centrifuge device and detection device on the workstation. However, this device also requires an additional centrifugation step, which is relatively cumbersome.
At present, the detection kit based on the immunochromatography principle is mainly used for detecting serum and plasma, the whole blood sample is treated by pretreatment including centrifugation, dilution and the like, operation steps and detection time are increased, and on the other hand, a blood filtering membrane is directly connected to a chromatography gasket. In addition, inconsistency in the chromatographic time due to individual difference of blood cells may also cause deviation of the detection result.
Therefore, how to design a rapid and simple detection device which overcomes the defect that a large amount of blood plasma is directly chromatographed is of great significance to the accurate detection of whole blood samples.
SUMMERY OF THE UTILITY MODEL
Not enough to prior art, the utility model aims at providing a whole blood sample test device for when solving the full blood test, the whole blood sample is direct to contact with the glass fiber membrane, causes red blood cell to pile up, leads to the red blood cell directly to dash the problem of going up the glass fiber membrane.
In order to achieve the purpose of the utility model, the utility model adopts the following technical proposal:
the utility model provides a whole blood sample detection device, including casing 1 down, casing 1 includes base 11 and support position 8 down, base 11 highly be not less than support position 8 and base 11's length is less than casing 1's length down to enclose into plasma collection chamber 7 jointly with support position 8, support position 8 is used for setting up red blood cell treatment layer 9, base 11 is used for placing the test paper strip, the test paper strip is including meeting in proper order and setting up glass fiber membrane 4, nitrocellulose membrane 5 and the membrane 6 that absorbs water on end card 10, the one end of glass fiber membrane 4 is good at the end card and is introduced in plasma collection chamber 7.
Preferably, the whole blood sample detection device further comprises an upper shell 2, the upper shell 2 is connected with the lower shell 1 in a chimeric way, and one end of the upper shell 2 is provided with a sample inlet 3 corresponding to the supporting part 8.
The erythrocyte treating layer 9 is generally provided with membranes which can filter erythrocytes, such as hemofiltration membranes.
The utility model provides a whole blood sample detection device has designed red blood cell processing layer at detect reagent box application of sample end, and its structure sets up support position 8 for the card shell is peripheral for placing and strain the blood membrane, then directly introduces plasma collection chamber 7 with glass fiber membrane 4. The function of this structure lies in having realized the separation of glass fiber membrane 4 and the hemofilter that the red blood cell handled the layer 9, after the application of sample, the red blood cell of whole blood sample is got into by introduction port 3, by setting up the hemofilter on the red blood cell handled layer 9 on the support site 8 and intercepting, plasma is separated plasma and is collected chamber 7, glass fiber membrane 4 directly absorbs the plasma after the separation through the chromatography and detects, and pass through nitrocellulose membrane 5 and absorb water membrane 6 in proper order, the chromatography influence that the individual difference of effective reduction red blood cell brought, simplify the testing procedure of whole blood sample, guarantee the uniformity of different sample results.
Preferably, the upper housing 2 is also provided with a plurality of fixing brackets 12, ensuring correct chromatographic drainage of the test strip.
Preferably, the fixing support 12 comprises a fixing support 12 corresponding to the position above one end of the glass fiber membrane 4 introduced into the plasma collection chamber 7.
Preferably, the fixing support 12 introduced into the plasma collection chamber 7 at a position above one end of the glass fiber membrane 4 is longer 12 relative to the rest fixing supports.
Preferably, the number of the fixing brackets 12 is 4.
In the utility model, the connection of the upper shell 2 and the lower shell 1 can be jogged and connected with the jogged hole correspondingly arranged on the lower shell 1 through the fixed bracket 12; therefore, not only the whole shell can be sealed, but also the glass fiber membrane 4, the nitrocellulose membrane 5, the water absorption membrane 6, the bottom card 10 and the like in the shell can be properly fixed through the fixing bracket.
In addition, the slight extrusion of sample notch has caused the drainage effect, improves erythrocyte filtration speed, and the fixed bolster 12 of everywhere has guaranteed that the upper and lower casing cooperates the back, and the test strip can correct chromatography drainage. Through the test, the device cooperates the chromatography test paper strip, can optimize the operation and experience, realizes more accurate detection. Wherein, a longer fixed bolster can prevent that the glass fiber membrane from upwards perk, introduces into the blood plasma collection chamber smoothly.
Compared with the prior art, the utility model discloses following beneficial effect has:
the whole blood sample detection device provided by the utility model, when the whole blood is detected, the glass fiber membrane is not directly contacted with the whole blood sample due to the separation of the glass fiber membrane and the red blood cell processing layer, therefore, the glass fiber membrane has no erythrocyte residue, the chromatographic time of different blood samples (especially different hematocrit) is closer, compared with a test card in which the blood filtration membrane directly participates in the lamination of the strips, the chromatographic influence caused by the individual difference of the erythrocyte can be effectively reduced, the detection steps of the whole blood sample are simplified, the consistency of the results of different samples is ensured, therefore, the whole blood detection CV and the sensitivity of the utility model are closer to the detection level of serum and plasma, the standard curve setting of the chromatography products is usually based on serum and plasma, and the fact that the whole blood detection is closer to the plasma means that the reading value is more accurate, so that the chromatography products can be used more conveniently and reliably for clinical application.
Drawings
FIG. 1 is a whole blood sample testing device provided in embodiment 1 of the present invention, wherein 1-lower casing, 2-upper casing, 3-sample inlet, 4-glass fiber membrane, 5-nitrocellulose membrane, 6-water absorbing membrane, 7-plasma collecting cavity, 8-supporting part, 9-red blood cell processing layer, 10-bottom card, 11-base.
FIG. 2 is a whole blood sample testing device provided by embodiment 2 of the present invention, wherein 1-lower casing, 2-upper casing, 3-sample inlet, 4-glass fiber membrane, 5-nitrocellulose membrane, 6-water absorbing membrane, 7-plasma collecting cavity, 8-supporting part, 9-red blood cell processing layer, 10-bottom card, 11-base, 12-fixing support.
FIG. 3 is a top view of the red blood cell treatment layer of the present invention, wherein 8-support part, 9-red blood cell treatment layer.
FIG. 4 is a whole blood sample testing device provided in embodiment 3 of the present invention, wherein 1-lower casing, 4-glass fiber membrane, 5-nitrocellulose membrane, 6-water absorbing membrane, 7-plasma collecting cavity, 8-supporting portion, 9-red blood cell processing layer, 10-bottom card, 11-base.
FIG. 5 is the conventional whole blood sample measuring device provided in example 4, wherein 1-lower case, 2-upper case, 3-sample inlet, 4-glass fiber membrane, 5-nitrocellulose membrane, 6-water absorbent membrane, 9-red blood cell processing layer, and 10-bottom card.
Detailed Description
The technical solution of the present invention will be further explained by the following embodiments. It should be understood by those skilled in the art that the described embodiments are merely provided to assist in understanding the present invention and should not be construed as specifically limiting the present invention.
Example 1
The embodiment provides a whole blood sample detection device
Whole blood sample detection device includes the shell structure who comprises lower casing 1 and last casing 2, go up casing 2 and lower casing 1 gomphosis and meet mutually, the one end of going up casing 2 is provided with introduction port 3 corresponding to support position 8, lower casing 1 includes base 11 and support position 8, the height of base 11 is not less than support position 8 and the length of base 11 is less than the length of lower casing 1, and enclose into plasma collection chamber 7 jointly with support position 8, support position 8 is used for setting up red blood cell treatment layer 9, base 11 is used for placing the test paper strip, the test paper strip is including meeting in proper order and setting up glass fiber membrane 4, cellulose nitrate membrane 5 and the membrane 6 that absorbs water on end card 10, the one end of glass fiber membrane 4 is longer than the end card and is introduced in the plasma collection chamber 7. The specific device diagram is shown in figure 1.
Example 2
The embodiment provides a whole blood sample detection device
The whole blood sample detection device comprises a shell structure consisting of a lower shell 1 and an upper shell 2, wherein the upper shell 2 is connected with the lower shell 1 in an embedded mode, one end of the upper shell 2 is provided with a sample inlet 3 corresponding to a supporting part 8, the lower shell 1 comprises a base 11 and the supporting part 8, the height of the base 11 is not lower than that of the supporting part 8, the length of the base 11 is smaller than that of the lower shell 1, the base and the supporting part 8 form a plasma collecting cavity 7 together in a surrounding mode, the supporting part 8 is used for arranging a red blood cell treatment layer 9, the base 11 is used for placing a test strip, the test strip comprises a glass fiber membrane 4, a nitrocellulose membrane 5 and a water absorption membrane 6 which are sequentially connected and arranged on a bottom card 10, one end of the glass fiber membrane 4 is longer than the bottom card and is led into the plasma collecting cavity 7, the upper shell 2 is provided with 4 fixing supports 12, wherein the fixing support 12 corresponding to the upper position of one end of the glass fiber membrane 4, a blood filter membrane is placed on the erythrocyte processing layer 9. The specific device diagram is shown in fig. 2.
Wherein a hemofiltration membrane is placed on the red blood cell processing layer 9, and an auxiliary support is designed around the red blood cell processing layer 9, and the top view of the red blood cell processing layer 9 is shown in fig. 3.
Example 3
The embodiment provides a whole blood sample detection device
Whole blood sample detection device includes casing 1 down, casing 1 includes base 11 and supporting site 8 down, base 11 highly be not less than supporting site 8 and base 11's length is less than casing 1's length down, and enclose into plasma collection chamber 7 jointly with supporting site 8, supporting site 8 is used for setting up erythrocyte treatment layer 9, base 11 is used for placing the test paper strip, the test paper strip is including meeting in proper order and setting up glass fiber membrane 4, cellulose nitrate membrane 5 and the membrane 6 that absorbs water on end card 10, the one end of glass fiber membrane 4 is long in end card and is introduced in the plasma is collected in the chamber 7. The specific device diagram is shown in fig. 4.
Example 4
The present embodiment provides a conventional whole blood sample testing device
The existing whole blood sample detection device comprises a shell structure consisting of a lower shell 1 and an upper shell 2, wherein the upper shell 2 is connected with the lower shell 1 in an embedded mode, one end of the upper shell 2 is provided with a sample inlet 3, the lower shell comprises a test strip and a red blood cell treatment layer 9, the test strip comprises a glass fiber membrane 4, a nitrocellulose membrane 5 and a water absorption membrane 6 which are sequentially connected and arranged on a bottom card 10, and one end of the red blood cell treatment layer 9 is connected with the glass fiber membrane 4. As shown in particular in fig. 5.
And (3) performance testing:
the whole blood sample testing device of example 2 was tested with the conventional whole blood sample testing device of example 4 by the following method: prepare the fluorescence test paper strip that cooperation device detected usefulness, detect in the immunofluorescence detector of terminal brain natriuretic peptide project, application of sample volume 100 μ L, respectively with the anticoagulant plasma of EDTA, the structure of whole blood of same sample source in embodiment 4 and the utility model discloses detect in the whole blood sample detection device of embodiment 2, 6 samples in total, experimental data is as shown in following table 1:
TABLE 1
Can know through data in table 1, the utility model provides a whole blood sample detection device has reduced the CV value that the product detected whole blood sample, and detects that data is closer plasma detection.
The applicant states that the present invention is illustrated by the above embodiments, but the present invention is not limited to the above process steps, i.e. the present invention is not meant to rely on the above process steps to implement the present invention. It should be clear to those skilled in the art that any improvement of the present invention is to the equivalent replacement of the selected raw materials, the addition of auxiliary components, the selection of specific modes, etc., all fall within the protection scope and disclosure scope of the present invention.
Claims (6)
1. The utility model provides a whole blood sample test device, its characterized in that, includes lower casing, lower casing includes base and support position, the length that highly is not less than support position and base of base is less than the length of lower casing to enclose into plasma collection chamber jointly with the support position, the support position is used for setting up red blood cell and handles the layer, the base is used for placing the test paper strip, the test paper strip is including meeting in proper order and setting up glass fiber membrane, nitrocellulose membrane and the membrane that absorbs water on the end card, the one end of glass fiber membrane is longer than the end card and is introduced in the plasma is collected in the chamber.
2. The whole blood sample test device according to claim 1, further comprising an upper housing, wherein the upper housing and the lower housing are connected in a jogged manner, and one end of the upper housing is provided with a sample inlet corresponding to the supporting part.
3. The whole blood sample testing device according to claim 2, wherein the upper housing is further provided with a plurality of fixing brackets.
4. The whole blood sample testing device according to claim 3, wherein said holding frame comprises a holding frame corresponding to a position above an end of the glass fiber membrane introduced into said plasma collection chamber.
5. The whole blood sample testing device according to claim 4, wherein the fixing support corresponding to the position above the one end of the glass fiber membrane introduced into the plasma collection chamber is longer than the remaining fixing supports.
6. The whole blood sample test device according to claim 3, wherein the number of the fixing brackets is 4.
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| CN201920677335.5U CN210136235U (en) | 2019-05-13 | 2019-05-13 | Whole blood sample detection device |
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| CN201920677335.5U CN210136235U (en) | 2019-05-13 | 2019-05-13 | Whole blood sample detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110007076A (en) * | 2019-05-13 | 2019-07-12 | 无锡博慧斯生物医药科技有限公司 | A kind of whole blood sample detection device and its detection method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110007076A (en) * | 2019-05-13 | 2019-07-12 | 无锡博慧斯生物医药科技有限公司 | A kind of whole blood sample detection device and its detection method |
| CN110007076B (en) * | 2019-05-13 | 2024-02-27 | 无锡博慧斯生物医药科技有限公司 | Whole blood sample detection device and detection method thereof |
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