CN218824304U

CN218824304U - Detection card suitable for immunochromatography detection reagent

Info

Publication number: CN218824304U
Application number: CN202222317600.3U
Authority: CN
Inventors: 袁占元
Original assignee: Xiamen Runkangyuan Biotechnology Co ltd
Current assignee: Xiamen Runkangyuan Biotechnology Co ltd
Priority date: 2022-08-30
Filing date: 2022-08-30
Publication date: 2023-04-07
Anticipated expiration: 2032-08-30

Abstract

The utility model discloses a detect card suitable for immunochromatography detect reagent relates to the detect card field, and the technical essential lies in: the method comprises the following steps: the detection card comprises a detection card main body and an auxiliary module, wherein the detection card main body comprises a sample adding hole; the auxiliary module is detachably inserted at one end of the detection card main body and comprises a filtering separator which is arranged opposite to the sample adding hole; wherein, the sample passes through the filtering separator and the sample adding hole in sequence and enters the detection card main body. The utility model discloses a filtration separator on the auxiliary module filters and separates the sample that awaits measuring, realize the preliminary treatment to the sample that awaits measuring, thereby make the interference thing in the sample that awaits measuring filtered in advance, the composition difference nature of different samples has been reduced, be favorable to the homogeneity of reaction and the reliability and the accuracy of result on the test paper, the auxiliary module can use and replace according to the sample selectivity that awaits measuring, can be suitable for such as immunochromatography detection reagent as new crown antibody detection reagent, promote the product suitability.

Description

Detection card suitable for immunochromatography detection reagent

Technical Field

The utility model relates to a test card field, concretely relates to test card suitable for immunochromatography detect reagent.

Background

The immunochromatography detection reagent is a reagent for in-vitro detection of human body samples in the processes of prediction, prevention, diagnosis, treatment monitoring, prognosis observation and health state evaluation of diseases, and the applied human body samples generally comprise various samples such as blood (serum, plasma and whole blood), urine, saliva, nasal secretion, excrement, hair, human tissue fluid and the like. Due to the difference of components and disease markers to be detected, the samples need to be preprocessed. Such as whole blood samples requiring filtration of red blood cells, hair samples requiring abrasive lysis extraction, metabolites requiring dissociation for release, etc.

Such as a D-dimer immunochromatographic assay reagent, the type of sample to which the reagent is applied is typically whole blood or plasma. It is well known that the fibrinolytic system is the most important anticoagulant system in the human body. When a fibrin clot forms, the fibrinolysis process begins and dissolves the fibrin clot to form various soluble fragments, D-dimers being one of the most important soluble fragments. The fibrinolytic system of the human body plays an important role in keeping normal permeability of the vascular wall, maintaining the flowing state of blood and repairing tissues. An increased level in the plasma of D-dimer indicates the presence of a secondary fibrinolytic process. The D-Dimer (D-Dimer) is a characteristic product of degradation of crosslinked fibrin, the appearance of the D-Dimer is a mark of fibrin deposition in a blood vessel wall, the D-Dimer is a key index of Deep Venous Thrombosis (DVT), pulmonary Embolism (PE) and Disseminated Intravascular Coagulation (DIC), the quantitative detection of the D-Dimer can quantitatively reflect the thrombolytic effect of a medicament and can be used for diagnosing and screening newly formed thrombus, and the D-Dimer has important significance for diagnosing and treating diseases of a fibrinolytic system, diseases related to the fibrinolytic system and monitoring thrombolytic therapy.

D-dimer is present in plasma or whole blood samples, and thus the type of sample to be tested for D-dimer is plasma or whole blood. The whole blood sample contains a large amount of red blood cells, and thus significantly interferes with the detection reaction of D-dimer. The technical scheme adopted conventionally is that an agglutination reaction of erythrocytes is added on a test paper, and the interception and filtration of the erythrocytes are realized through the agglutination reaction of antigen and antibody. However, the agglutination reaction of the red blood cells on the test paper is relied on singly, the antibody with higher concentration is needed to achieve the expected agglutination interception and filtration effect, and meanwhile, the antibody with higher concentration can be partially chromatographed on the nitrocellulose membrane along with the liquid, so that a certain degree of interference is brought to the immunodetection of the D-dimer. Meanwhile, part of erythrocytes which are not completely agglutinated, intercepted and filtered are chromatographed on the nitrocellulose membrane with a certain probability, so that the immunodetection reaction of the D-dimer is interfered, and the accuracy and precision of the final test result are obviously interfered. The conventional D-dimer detection card on the market is only used as a protective jacket of the detection test paper, and has no pretreatment function on a sample to be detected.

The inventor is realizing the technical scheme of the utility model, finds that there are following technical problems in current detection card:

the conventional D-dimer detection card does not have a pretreatment function on a sample to be detected, and the conventional method of adding excessive anti-erythrocyte antibodies on the detection test paper to realize agglutination separation of erythrocytes is adopted, so that the excessive anti-erythrocyte antibodies and free erythrocytes can interfere with the test result of the detection test paper, and the stability and accuracy of the test result are reduced.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model provides a detection card suitable for immunochromatography detect reagent solves current detection card at least and does not have the problem to sample preprocessing function and can show the interference that reduces red blood cell and bring the testing result.

(II) technical scheme

In order to solve the technical problem, the utility model provides a following technical scheme:

a test card suitable for use with an immunochromatographic test reagent, comprising:

the detection card comprises a detection card main body, a detection card body and a detection card body, wherein the detection card main body comprises a sample adding hole;

the auxiliary module is detachably inserted at one end of the detection card main body and comprises a filtering separator which is arranged opposite to the sample adding hole;

wherein, the sample passes through the filtering separator and the sample adding hole in sequence to enter the detection card main body.

In a further set scheme, the filtering separator is one or more of non-woven fabrics, glass fiber materials with porous structures, nylon membranes, nitrocellulose membranes and cellulose acetate membranes.

In a further arrangement, the auxiliary module comprises a housing, the housing comprises an insertion groove and a mounting hole communicated with the insertion groove, the detection card body is arranged in the insertion groove, and the filtering separator is arranged in the mounting hole.

In a further arrangement, the shell further comprises an annular protrusion positioned on the wall of the insertion groove, and the detection card main body further comprises an annular groove positioned on the periphery of the sample adding hole, wherein the annular groove is used for the annular protrusion to rotate; the shell also comprises a holding part used for holding fingers.

In a further arrangement, the housing further comprises a positioning bump, the positioning bump is made of an elastic material and is arranged around the periphery of the annular protrusion, and the detection card main body further comprises a positioning concave hole arranged around the periphery of the annular groove; when the positioning convex points are matched with the positioning concave holes, the auxiliary module is relatively fixed with the detection card main body.

In a further arrangement, the end face of one end of the detection card body inserted into the insertion groove is an inclined plane, and the inclined plane is a straight inclined plane or an arc inclined plane.

(III) advantageous effects

Compared with the prior art, the utility model provides a detection card possesses following beneficial effect:

(1) The detection card is provided with the auxiliary module on the detection card main body, and filters and separates a sample to be detected through the filtering separator on the auxiliary module, so that the sample to be detected is pretreated, interference objects in the sample to be detected are filtered in advance, the component differences of different samples are reduced, and the uniformity of reaction on the detection test paper and the reliability and accuracy of results are facilitated.

(2) The auxiliary module is detachably inserted into the detection card main body, can be selectively used and replaced according to a sample to be detected, and can be detached from the auxiliary module on the sample to be detected without filtering and separating treatment, so that the detection cost is reduced, and the product applicability is improved.

Drawings

FIG. 1 is a schematic view of a test card suitable for an immunochromatographic test reagent in example 1;

FIG. 2 is a front view of a test card suitable for an immunochromatographic test reagent in example 2;

FIG. 3 is a schematic view of section A of FIG. 2;

FIG. 4 is a schematic view of section B of FIG. 3;

FIG. 5 is a disassembled view of the test card suitable for the immunochromatographic test reagent in example 2.

Reference numerals: the detection card comprises a detection card main body 1, an auxiliary module 2, a sample adding hole 10, an annular groove 11, a positioning concave hole 12, an inclined plane 13, a shell 20, a filter separator 21, a plug-in groove 201, a mounting hole 202, an annular protrusion 203, a pinching part 204 and a positioning convex point 205.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

Example 1: referring to fig. 1, fig. 1 is a schematic diagram of a test card in embodiment 1.

The detection card suitable for the immunochromatography detection reagent of the embodiment comprises: the card body 1 and the auxiliary module 2 are detected.

The test card body 1 includes a loading hole 10.

The detection card body 1 is used for detecting samples, the type of the detection card body 1 can be selected according to the type of the detection samples, and the samples to be detected enter the detection card body 1 through the sample adding holes 10 for detection.

The auxiliary module 2 is detachably inserted into one end of the detection card body 1, and includes a filter separator 21, and the filter separator 21 is disposed opposite to the sample application hole 10.

The auxiliary module 2 preprocesses the sample to be tested through the filtering separator 21, filters and separates the interferent in the sample to be tested, and the preprocessed sample to be tested flows into the sample adding hole 10.

Wherein the sample sequentially passes through the filtering separator 21 and the sample application hole 10 to enter the test card body 1.

In one embodiment of the filtering separator 21, the filtering separator 21 is one or more of a non-woven fabric, a glass fiber material with a porous structure, a nylon membrane, a nitrocellulose membrane, and a cellulose acetate membrane, and the filtering separator 21 is disposed in a layered shape, a mesh shape, and the like.

The filtering and separating function of the filtering and separating device 21 may be realized by physical pore size filtering and separating, or may be realized by biochemical reaction assistance.

The detection card of above technical scheme sets up auxiliary module 2 on detecting card main part 1 to filter and separate the sample that awaits measuring through filter separator 21 on auxiliary module 2, realize the preliminary treatment to the sample that awaits measuring, thereby make the interferent in the sample that awaits measuring filtered in advance, reduced the composition difference of different samples, be favorable to the homogeneity of reaction and the reliability and the accuracy of result on the test paper. Taking the D-dimer detection card as an example, on the basis of the existing detection card, the pretreatment of the whole blood sample to be detected is realized through the filtering and separating functions of the auxiliary module 2 on the red blood cells in the whole blood sample to be detected, so that the red blood cells of the whole blood sample to be detected are filtered in advance, the component differences of different whole blood samples are reduced, the whole blood sample is close to the plasma sample, and the uniformity of immunoreaction on the detection test paper and the reliability of the result are facilitated. The auxiliary module 2 belongs to pretreatment of a whole blood sample, and compared with in-situ separation and filtration of a sample pad of a conventional detection test paper, the auxiliary module 2 disclosed by the invention belongs to ex-situ separation and filtration of the sample, so that the interference of sample pretreatment operation on immune reaction on the detection test paper is effectively avoided.

Simultaneously, the grafting of auxiliary module 2 detachable is on detecting card main part 1, and auxiliary module 2 can use and replace according to the sample selectivity that awaits measuring, if can pull down auxiliary module 2 on the sample that awaits measuring that need not the filtering separation processing, reduces and detects the cost, promotes the product suitability.

Example 2: referring to fig. 2, 3, 4 and 5, fig. 2 is a front view of the detection card in embodiment 2, fig. 3 is a schematic diagram of a section a in fig. 2, fig. 4 is a schematic diagram of a section B in fig. 3, and fig. 5 is a schematic diagram of the detection card in embodiment 2 after being detached.

The detection card of the embodiment is configured as follows:

in an implementation mode that the auxiliary module 2 and the detection card main body 1 are detachably connected in a plugging manner, the auxiliary module 2 comprises a shell 20, the shell 20 can be of an integrally formed structure or a split structure, the shell 20 comprises a plugging groove 201 and a mounting hole 202 communicated with the plugging groove 201, the filter separator 21 is arranged in the mounting hole 202 in a bonding or filling manner, the detection card main body 1 is connected in the plugging groove 201 in a plugging manner during installation, and the installation is convenient and rapid.

In order to enable the auxiliary module 2 to rotate around the detection card main body 1 and serve as a part for taking the detection card, the shell 20 further comprises an annular protrusion 203 located on the groove wall of the insertion groove 201, the detection card main body 1 further comprises an annular groove 11 located on the periphery of the sample adding hole 10, the annular groove 11 is used for the annular protrusion 203 to rotate, namely when the detection card main body 1 is inserted into the insertion groove 201 of the auxiliary module 2, the annular protrusion 203 is embedded into the annular groove 11 to be in rotating fit, the shell 20 further comprises a holding part 204, the holding part 204 is used for holding fingers, the auxiliary module 2 can rotate relative to the main body, the rotated two are relatively fixed through a tight connection or positioning structure, an operator can take the detection card through the auxiliary module 2, and the taking angle can be adjusted according to operation habits or needs.

In an embodiment of the above positioning structure, the housing 20 further includes a positioning bump 205, the positioning bump 205 is made of an elastic material and is disposed around the periphery of the annular bump 203, the positioning bump 205 may be a rubber bump fixed on the inner side of the housing 20, and the detection card main body 1 further includes a positioning concave hole 12 disposed around the periphery of the annular groove 11; the number and the interval between the positioning bumps 205 and the positioning holes 12 can be set as required, for example, when the auxiliary module 2 and the detection card body 1 are provided with matching points at vertical and horizontal positions, and the auxiliary module 2 and the detection card body 1 are limited by the positioning bumps 205 in the rotation direction and are relatively fixed when the auxiliary module and the detection card body 1 are matched; so, operating personnel can be when rotating supplementary module 2 through location bump 205 and the cooperation location of location shrinkage pool 12, and the operation has the gear and feels, and it is more convenient to use.

In order to facilitate the insertion of the detection card body 1 into the auxiliary module 2, in some embodiments, an end surface of the detection card body 1 inserted into the insertion slot 201 is an inclined surface 13, and the inclined surface 13 is a straight inclined surface or an arc inclined surface.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A test card adapted for use with an immunochromatographic test reagent, comprising:

2. The detection card of claim 1, wherein the auxiliary module comprises a housing, the housing comprises a slot and a mounting hole communicating with the slot, the detection card body is disposed in the slot, and the filter separator is disposed in the mounting hole.

3. The test card of claim 2, wherein the housing further comprises an annular protrusion formed on a wall of the insertion groove, and the test card body further comprises an annular recess formed on a periphery of the sample application hole, the annular recess being adapted to allow the annular protrusion to rotate; the shell also comprises a holding part used for holding fingers.

4. The test card of claim 3, wherein the housing further comprises a positioning protrusion, the positioning protrusion is made of an elastic material and is disposed around the periphery of the annular protrusion, and the test card body further comprises a positioning recess disposed around the periphery of the annular recess; when the positioning convex points are matched with the positioning concave holes, the auxiliary module is relatively fixed with the detection card main body.

5. The reagent cartridge of claim 2, wherein the end surface of the cartridge body inserted into the insertion groove is a slope, and the slope is a straight slope or a circular arc slope.