CN106840828A - The method and separator of quick separating blood plasma in a kind of micro whole blood - Google Patents
The method and separator of quick separating blood plasma in a kind of micro whole blood Download PDFInfo
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Abstract
The invention provides the method and separator of quick separating blood plasma in a kind of micro whole blood, the method of quick separating blood plasma in the micro whole blood, by sample, overlapping for direction adsorbs the physics accumulation for reducing red blood cell in separation process from lower to upper, from 3 μm of polyethersulfone resin (PES) films by sulfonation hydrophily treatment unsymmetric structure in aperture, it is effectively isolated red blood cell and improves blood plasma transmitance again, the plasma adsorption area of low density glass fiber is easy to draw the blood plasma after separating, and improves the uptake of blood plasma；Separator used includes A areas Plasma absorptions material, B areas polyethersulfone resin (PES) film, C areas composite fibre and D areas glass fibre, be not required to external equipment, using more portable, overlapping for direction adsorbs the physics accumulation for reducing red blood cell in separation process from lower to upper for mechanical power destruction red blood cell, sample, the plasma adsorption area of low density glass fiber is easy to draw the blood plasma after separating, and high-purity blood plasma is efficiently separated in a short time.
Description
Technical field
The present invention relates to a kind of method of quick separating blood plasma in biological sample separation technology field, especially micro whole blood
And separator.
Background technology
It is to stand the whole blood containing anti-coagulants at this stage for the separation of blood plasma, the Action of Gravity Field by red blood cell enters
Row sedimentation separation obtains supernatant for blood plasma；Or the whole blood sample containing anti-coagulants is centrifuged, isolated supernatant
It is blood plasma；CN201380041858.6 patents of invention are separated with capillary using film to blood plasma；CN201310254336.6
Patent of invention is separated by chemically treated microchannel using PDMS chips to blood plasma.
For above-mentioned prior art means, often have the following disadvantages：
Standing separation needs the long time treatment isolated blood plasma of ability.
Although centrifugation reduces disengaging time, but needs that by centrifuge blood plasma point could be realized after sample collection
From centrifuge is needed to be unable to portable use installed in horizontal fixed venue, and centrifugal force may cause damage to red blood cell to be caused
Haemolysis.
CN201380041858.6 patents of invention provide a kind of portable plasma separating unit, the device by film directly with entirely
Blood sample is contacted, vertical direction filtering.Because Action of Gravity Field red blood cell can be deposited to film bottom in filter process, cause aperture
Footpath film is blocked, and directly affects separative efficiency.
The microchannel that CN201310254336.6 inventions are used can just use after needing complicated Chemical Pretreatment, due to
Red blood cell system is divided into pronormoblast, early erythroblast, rubricyte, metarubricyte, granulophilocyte and red blood cell, carefully
Born of the same parents' diameter is about at 6-10 microns, but a diameter of 0.5-50 microns that the microchannel of the device allows, the meeting in separation process
Cause to be mixed with some red blood cells in separate blood plasma.
Therefore, search out and be not required to centrifuge or external instrument and can be independently operated, and can efficiently separate high-purity in a short time
The method and device of blood plasma, with important application value.
The content of the invention
The technical problems to be solved by the invention are the method for providing quick separating blood plasma in a kind of micro whole blood.
The technical problems to be solved by the invention are to provide a kind of separation for realizing quick separating blood plasma in micro whole blood
Device.
In order to solve the above technical problems, the technical scheme is that：
The method of quick separating blood plasma, comprises the following steps that in a kind of micro whole blood：
(1) take Plasma absorption material, by sulfonation hydrophily process 0.03-10 μm of aperture unsymmetric structure polyethers
Sulphone resin (PES) film, contain 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2 (ethylenediamines
Tetraacethyl dipotassium) or EDTA-NA (disodium ethylene diamine tetraacetate) glass fibre, contain 0.1mg/mL-10mg/mL red blood cell lists
The glass of clonal antibody and 0-2.2mg/mL EDTA-K2 (EDTAP dipotassium ethylene diamine tetraacetate) or EDTA-NA (disodium ethylene diamine tetraacetate)
Glass fiber successively place by edge superposition, and the Plasma absorption material is placed in the superiors, the Plasma absorption material be glass fibre,
Polyester, composite fibre or pure cotton filter paper, wherein,
Plasma absorption material, referred to as A areas；
The polyethersulfone resin of the unsymmetric structure in 0.03-10 μm of the aperture processed by sulfonation hydrophily
(PES) film, referred to as B areas；
Contain 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2 (ethylenediamine tetra-acetic acids
Dipotassium) or EDTA-NA (disodium ethylene diamine tetraacetate) composite fibre, referred to as C areas；
Contain 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2 (ethylenediamine tetra-acetic acids
Dipotassium) or EDTA-NA (disodium ethylene diamine tetraacetate) glass fibre, referred to as D areas；
(2) the red blood cell monoclonal antibody and 0- of whole blood sample 0.1mg/mL-10mg/mL concentration pre-coated with D areas first
The glass fibre reaction of 2.2mg/mL EDTA anti-coagulants, EDTA ensure that whole blood will not occur aggegation, whole blood in separation process
In red blood cell specific immunological response can occur with red blood cell monoclonal antibody, due to the irregular knot of glass fibre
Structure, most of red blood cell can be adsorbed in D areas；
(3) the red blood cell monoclonal of small part red blood cell and blood plasma 0.1mg/mL-10mg/mL concentration pre-coated with C areas again
The composite fibre reaction of antibody and EDTA anti-coagulants, most red blood cells are adsorbed by the reaction in D areas and C areas, due to
The composite fibre material of D areas glass fibre is better than from mobility and water imbibition, promotes red blood cell and blood plasma quick separating；
(4) a small amount of free red blood cell is trapped in C areas by the polyethersulfone resin membrane in B areas by the physical action of molecular sieve,
Only blood plasma can pass through, and finally adsorb in A areas Plasma absorption material.
Preferably, in above-mentioned micro whole blood quick separating blood plasma method, the aperture of the polyethersulfone resin membrane is 3 μm.
Preferably, in above-mentioned micro whole blood quick separating blood plasma method, contain 2.2mg/ in the glass fibre in the C areas
mL EDTA-K2。
Preferably, in above-mentioned micro whole blood quick separating blood plasma method, contain 0.1mg/ in the glass fibre in the D areas
ML red blood cell monoclonal antibodies.
A kind of separator for realizing quick separating blood plasma in micro whole blood, including A areas Plasma absorptions material, B areas
Polyethersulfone resin (PES) film, C areas composite fibre and D areas glass fibre, and PVC board and shell, the PVC board and shell are matched somebody with somebody
Close fixation and form box-like hollow structure, the A areas Plasma absorption material, B areas polyethersulfone resin (PES) film, C areas composite fibre and
D areas glass fibre is placed in the box-like hollow structure and edge superposition is positioned in the PVC board successively, and A areas blood plasma is inhaled
Receive material and be placed in the superiors, to be referred to as blood plasma temporary for non-side Chong Die with B areas polyethersulfone resin membrane on the A areas Plasma absorption material
Pond, is referred to as Plasma absorption area, being set on the shell on A areas Plasma absorption material near side Chong Die with B areas polyethersulfone resin membrane
There are three through holes, the surface of blood plasma scratch pool, Plasma absorption area and D areas glass fibre is correspondingly arranged in respectively, wherein,
Plasma absorptions material in A areas is glass fibre, polyester, composite fibre or pure cotton filter paper,
B areas polyethersulfone resin (PES) film is the unsymmetric structure in 0.03-10 μm of the aperture processed by sulfonation hydrophily
Polyethersulfone resin membrane,
C areas glass fibre is to contain 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2
The composite fibre of (EDTAP dipotassium ethylene diamine tetraacetate) or EDTA-NA (disodium ethylene diamine tetraacetate),
D areas glass fibre is to contain 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2
The glass fibre of (EDTAP dipotassium ethylene diamine tetraacetate) or EDTA-NA (disodium ethylene diamine tetraacetate).
Preferably, the above-mentioned separator for realizing quick separating blood plasma in micro whole blood, the polyethersulfone resin membrane
Aperture be 3 μm.
Preferably, the above-mentioned separator for realizing quick separating blood plasma in micro whole blood, in C areas glass fibre
Contain 2.2mg/mL EDTA-K2.
Preferably, the above-mentioned separator for realizing quick separating blood plasma in micro whole blood, in D areas glass fibre
Contain 0.1mg/mL red blood cell monoclonal antibodies.
The beneficial effects of the invention are as follows：
The method of quick separating blood plasma in the micro whole blood, by sample from lower to upper direction overlap adsorb reduce
The physics of red blood cell is piled up in separation process, from the 3 μm of polyether sulfone trees by sulfonation hydrophily treatment unsymmetric structure in aperture
Fat (PES) film, that is, be effectively isolated red blood cell and improve blood plasma transmitance again, and the plasma adsorption area of low density glass fiber is easy to draw
Blood plasma after separation, improves the uptake of blood plasma；Separator small volume used, low production cost, be not required to external equipment, should
With more portable, the overlap absorption in mechanical power destruction red blood cell, sample direction from lower to upper reduces red thin in separation process
The physics of born of the same parents is piled up, and the plasma adsorption area of low density glass fiber is easy to draw the blood plasma after separating, and efficiently divides in a short time
From high-purity blood plasma.
Brief description of the drawings
Fig. 1 is that the internal structure for realizing the separator of quick separating blood plasma in micro whole blood of the present invention is illustrated
Figure；In figure, 1-S1 pad 2-S2 pad 3-PES film 4-AB pads
Fig. 2 is that the external structure for realizing the separator of quick separating blood plasma in micro whole blood of the present invention is illustrated
Figure, modeling card is provided with tri- through holes of A, B, C.
Specific embodiment
Technical scheme of the present invention is further described with reference to specific embodiment.
" the red blood cell monoclonal antibody " mentioned in following embodiments refers to mouse anti-human RBC's monoclonal antibody, is commercialization
Biological raw material, purchased from Shenzhen City Fapon Biotech Co., Ltd.
Embodiment 1
1. material：
Red blood cell monoclonal antibody, EDTA-K2, composite fibre, glass fibre, 0.03 μm, 3.00 μm and 10.00 μm PES
Film, PVC board, it is known that (whole blood sample P4 is negative whole blood sample to the EDTA-K2 anticoagulated whole blood samples P4 of packed cell volume, is come
From the negative whole blood sample of Tianjin Hua Tai hospitals).
2. method
Composite fibre and glass fibre are immersed in and contain 0.1mg/mL red blood cells monoclonal antibody and 2.2mg/mL
In the 0.01M PH7.4PBS buffer solutions of EDTA-K2, fully dried 12 hours at 45 DEG C after immersion.Glass fibre note after treatment
It is S1 pads, the composite fibre after treatment is designated as S2 pads, and untreated glass fibre is designated as AB pads.
As shown in Figure 1 and Figure 2, according to S1 pads 1, S2 pads 2, PES films 3, AB pads 4 order, 4 materials are overlapped successively viscous
It is attached in PVC board, the 3 kinds of semi-finished product of different compositions according to PES films.Semi-finished product are cut into 4mm bar shapeds and are attached to formation during modeling blocks
Separator.The modeling card is provided with three through hole A holes, B holes and C holes, wherein, C holes are arranged at S1 pads surface, A holes and B holes
It is arranged at directly over AB pads, while A holes and C holes are respectively arranged at the both sides in B holes.
P4 samples are chosen to detect the separative efficiency of separator.100 μ L whole blood samples are added to the C of separator
Hole, using pipettor from separator B holes draws blood plasma and measures the volume of blood plasma, according to formula separation after standing 3 minutes
Efficiency=(1- packed cell volumes) × sample size ÷ separated volumes, calculates the separation rate of blood plasma.
3. result
Experimental result is shown in Table 1, as a result shows, 0.03 μm of the too small influence separative efficiency of PES membrane apertures, 10 μm of PES films
The larger blood plasma separative efficiency highest in aperture, but have very much the blood plasma that some red blood cells are also exuded to after separating greatly due to aperture
In, 3 μm of PES films can be adequately isolated the infiltration of free red blood cell, and equally have separative efficiency higher.
Table 1
Embodiment 2
1. material：
Red blood cell monoclonal antibody, EDTA-K2, composite fibre, glass fibre, 3 μm of PES films, PVC board, it is known that red blood cell
The EDTA-K2 anticoagulated whole blood samples P4 of hematocrit.
2. method
Composite fibre and glass fibre are immersed in and contain 0.1mg/mL red blood cells monoclonal antibody and 2.2mg/mL
In the 0.01M PH7.4PBS buffer solutions of EDTA-K2, fully dried 12 hours at 45 DEG C after immersion.Glass fibre note after treatment
It is S1 pads, the composite fibre after treatment is designated as S2 pads, and untreated glass fibre is designated as AB pads.
According to S1 pads, S2 pads, PES films, AB pads order, 4 materials are overlapped successively be pasted onto in PVC board composition half into
Product.Semi-finished product are respectively cut and form separator (structure for the bar shaped of 2mm, 4mm and 8mm is attached in different size of modeling card
Referring to embodiment 1).Choose the separative efficiency of P4 pattern detection separators.50,100 and 200 μ L whole blood samples are added respectively
Using pipettor from separator B holes blood plasma is drawn to the C holes of separator, after standing 3 minutes and measure the volume of blood plasma,
According to formula separative efficiency=(1- packed cell volumes) × sample size ÷ separated volumes, the separation rate of blood plasma is calculated.
3. result
Experimental result is shown in Table 2-4, as a result shows, separative efficiency is up to when the separator of 2mm separates 50 μ L samples
60.57%, excessive sample only can rest on well.Separative efficiency highest when the separator of 4mm separates 100 μ L samples
It is 61.15%, sample point can not be carried out because separator sorptive material sample size in itself can cause separator very little
From, and the sample of excess only can rest on well.Separative efficiency is up to when the separator of 8mm separates 200 μ L samples
63.48%, sample separation can not be carried out because separator sorptive material sample size in itself can cause separator very little,
And the not enough meeting of sample size influences the separative efficiency of sample.
The testing result of the 2mm separators of table 2
The testing result of the 4mm separators of table 3
The testing result of the 8mm separators of table 4
Embodiment 3
1. material：
Red blood cell monoclonal antibody, EDTA-K2, low density glass fiber, high-density glass fiber, 3 μm of PES films, PVC
Plate, it is known that negative EDTA-K2 anticoagulated whole bloods sample P1, P2, P3, P4, P5 of packed cell volume (are negative whole blood sample, come
From the negative whole blood sample of Tianjin Hua Tai hospitals).
2. method
Composite fibre and glass fibre are immersed in and contain 0.1mg/mL red blood cells monoclonal antibody and 2.2mg/mL
In the 0.01M PH7.4PBS buffer solutions of EDTA-K2, fully dried 12 hours at 45 DEG C after immersion.Glass fibre note after treatment
It is S1 pads, the composite fibre after treatment is designated as S2 pads, and untreated glass fibre is designated as AB pads.
According to S1 pads, S2 pads, PES films, AB pads order, 4 materials are overlapped successively be pasted onto in PVC board composition half into
Product.Semi-finished product are cut into 4mm bar shapeds and are attached to formation separator during modeling blocks (structure is referring to embodiment 1).Choose known red thin
The separative efficiency of the EDTA-K2 anticoagulated whole blood pattern detection separators of born of the same parents' hematocrit.100 μ L whole blood samples are added to separation dress
The C holes put, using pipettor from separator B holes draw blood plasma and measure the volume of blood plasma, according to formula after standing 3 minutes
Separative efficiency=(1- packed cell volumes) × sample size ÷ separated volumes, calculates the separation rate of blood plasma.
3. result
Experimental result is shown in Table 5, as a result shows, packed cell volume is divided in the blood sample of 0.38-0.51 scopes by blood plasma
After device separation, the separative efficiency of blood plasma is about 62.46%, resists by 3 μm of PES films, containing 0.1mg/mL red blood cell monoclonals
The S1 pads of body, the S2 pads containing 2.2mg/mL EDTA-K2, the plasma separating unit of AB pads composition are applied to different red blood cell pressures
Long-pending blood sample.
Table 5
It is above-mentioned with reference to embodiment the method and separator of quick separating blood plasma in a kind of micro whole blood are carried out it is detailed
Thin description, is illustrative rather than limited, can include several embodiments according to limited scope, therefore do not taking off
Changing and modifications under present general inventive concept, should belong within protection scope of the present invention.
Claims (8)
1. in a kind of micro whole blood quick separating blood plasma method, it is characterised in that：Comprise the following steps that：
(1) take Plasma absorption material, by sulfonation hydrophily process 0.03-10 μm of aperture unsymmetric structure polyether sulfone tree
Adipose membrane, the glass containing 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2 or EDTA-NA
Fiber, the glass containing 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2 or EDTA-NA
Fiber successively place by edge superposition, and the Plasma absorption material is placed in the superiors, and the Plasma absorption material is glass fibre, gathers
Ester, composite fibre or pure cotton filter paper, wherein,
Plasma absorption material, referred to as A areas；
The polyethersulfone resin membrane of the unsymmetric structure in 0.03-10 μm of the aperture processed by sulfonation hydrophily, referred to as B areas；
Mixing containing 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2 or EDTA-NA is fine
Dimension, referred to as C areas；
Glass fibers containing 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2 or EDTA-NA
Dimension, referred to as D areas；
(2) the red blood cell monoclonal antibody and 0- of whole blood sample 0.1mg/mL-10mg/mL concentration pre-coated with D areas first
The glass fibre reaction of 2.2mg/mL EDTA anti-coagulants, EDTA ensure that whole blood will not occur aggegation, whole blood in separation process
In red blood cell specific immunological response can occur with red blood cell monoclonal antibody, due to the irregular knot of glass fibre
Structure, most of red blood cell can be adsorbed in D areas；
(3) the red blood cell monoclonal antibody of small part red blood cell and blood plasma 0.1mg/mL-10mg/mL concentration pre-coated with C areas again
Composite fibre with EDTA anti-coagulants is reacted, and is adsorbed most red blood cells by the reaction in D areas and C areas, due to selecting
Mobility and water imbibition are better than the composite fibre material of D areas glass fibre, promote red blood cell and blood plasma quick separating；
(4) a small amount of free red blood cell is trapped in C areas by the polyethersulfone resin membrane in B areas by the physical action of molecular sieve, only
Blood plasma can pass through, and finally adsorb in A areas Plasma absorption material.
2. in micro whole blood according to claim 1 quick separating blood plasma method, it is characterised in that：The polyether sulfone tree
The aperture of adipose membrane is 3 μm.
3. in micro whole blood according to claim 1 quick separating blood plasma method, it is characterised in that：The glass in the C areas
Contain 2.2mg/mL EDTA-K2 in glass fiber.
4. in micro whole blood according to claim 1 quick separating blood plasma method, it is characterised in that：The glass in the D areas
Contain 0.1mg/mL red blood cell monoclonal antibodies in glass fiber.
5. a kind of separator for realizing quick separating blood plasma in micro whole blood described in claim 1, it is characterised in that：Bag
Include A areas Plasma absorptions material, B areas polyethersulfone resin membrane, C areas composite fibre and D areas glass fibre, and PVC board and shell, institute
State PVC board and shell coordinates fixation to form box-like hollow structure, the A areas Plasma absorption material, B areas polyethersulfone resin membrane, C areas
Composite fibre and D areas glass fibre are placed in the box-like hollow structure and edge superposition is positioned in the PVC board successively, described
A areas Plasma absorption material is placed in the superiors, and non-side Chong Die with B areas polyethersulfone resin membrane claims on the A areas Plasma absorption material
It is blood plasma scratch pool, Plasma absorption area, institute is referred to as near side Chong Die with B areas polyethersulfone resin membrane on A areas Plasma absorption material
State shell and be provided with three through holes, just going up for blood plasma scratch pool, Plasma absorption area and D areas glass fibre is correspondingly arranged in respectively
Side, wherein,
Plasma absorptions material in A areas is glass fibre, polyester, composite fibre or pure cotton filter paper,
B areas polyethersulfone resin membrane is the polyether sulfone tree of the unsymmetric structure in 0.03-10 μm of the aperture processed by sulfonation hydrophily
Adipose membrane,
C areas glass fibre be containing 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2 or
The composite fibre of EDTA-NA,
D areas glass fibre be containing 0.1mg/mL-10mg/mL red blood cells monoclonal antibody and 0-2.2mg/mL EDTA-K2 or
The glass fibre of EDTA-NA.
6. the separator for realizing quick separating blood plasma in micro whole blood according to claim 5, it is characterised in that：
The aperture of the polyethersulfone resin membrane is 3 μm.
7. the separator for realizing quick separating blood plasma in micro whole blood according to claim 5, it is characterised in that：
Contain 2.2mg/mL EDTA-K2 in C areas glass fibre.
8. the separator for realizing quick separating blood plasma in micro whole blood according to claim 5, it is characterised in that：
Contain 0.1mg/mL red blood cell monoclonal antibodies in D areas glass fibre.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108152177A (en) * | 2017-12-11 | 2018-06-12 | 三诺生物传感股份有限公司 | A kind of method of quick detection hematocrit |
| WO2020220157A1 (en) * | 2019-04-27 | 2020-11-05 | 南京岚煜生物科技有限公司 | Whole blood filtering method and filter membrane structure for whole blood filtering |
| CN116731962A (en) * | 2023-08-14 | 2023-09-12 | 天津中新科炬生物制药股份有限公司 | Kit and method for separating red blood cells from whole blood |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201177631Y (en) * | 2008-02-01 | 2009-01-07 | 陈莉莉 | Whole blood detection type immunity-chromatography device |
| CN101625357A (en) * | 2008-06-03 | 2010-01-13 | 卓安医疗科技有限公司 | Apparatus and method for rapid detection |
| CN101750244A (en) * | 2008-10-13 | 2010-06-23 | 艾博生物医药(杭州)有限公司 | Method for separating red cells from blood sample and application |
| CN101839908A (en) * | 2010-05-28 | 2010-09-22 | 李金波 | Quantitative detection device and detection method of biological fluid samples |
| CN101862564A (en) * | 2010-03-16 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Micro-blood sample pretreatment system and preparation process thereof |
| CN201658893U (en) * | 2010-03-16 | 2010-12-01 | 苏州市玮琪生物科技有限公司 | Micro-blood sample pretreatment system |
| CN104428651A (en) * | 2012-04-20 | 2015-03-18 | 斯利普奇普公司 | Fluidic devices and systems for sample preparation or autonomous analysis |
| CN105209880A (en) * | 2013-03-15 | 2015-12-30 | 赛拉诺斯股份有限公司 | Methods and devices for sample collection and sample separation |
| CN105445083A (en) * | 2015-12-21 | 2016-03-30 | 丹娜（天津）生物科技有限公司 | Centrifugation-free whole-blood processing module |
| CN105722576A (en) * | 2013-11-14 | 2016-06-29 | 生物梅里埃公司 | Novel filter medium for obtaining plasma, and associated filtration device and method |
-
2017
- 2017-03-29 CN CN201710196864.9A patent/CN106840828B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201177631Y (en) * | 2008-02-01 | 2009-01-07 | 陈莉莉 | Whole blood detection type immunity-chromatography device |
| CN101625357A (en) * | 2008-06-03 | 2010-01-13 | 卓安医疗科技有限公司 | Apparatus and method for rapid detection |
| CN101750244A (en) * | 2008-10-13 | 2010-06-23 | 艾博生物医药(杭州)有限公司 | Method for separating red cells from blood sample and application |
| CN101862564A (en) * | 2010-03-16 | 2010-10-20 | 苏州市玮琪生物科技有限公司 | Micro-blood sample pretreatment system and preparation process thereof |
| CN201658893U (en) * | 2010-03-16 | 2010-12-01 | 苏州市玮琪生物科技有限公司 | Micro-blood sample pretreatment system |
| CN101839908A (en) * | 2010-05-28 | 2010-09-22 | 李金波 | Quantitative detection device and detection method of biological fluid samples |
| CN104428651A (en) * | 2012-04-20 | 2015-03-18 | 斯利普奇普公司 | Fluidic devices and systems for sample preparation or autonomous analysis |
| CN105209880A (en) * | 2013-03-15 | 2015-12-30 | 赛拉诺斯股份有限公司 | Methods and devices for sample collection and sample separation |
| CN105722576A (en) * | 2013-11-14 | 2016-06-29 | 生物梅里埃公司 | Novel filter medium for obtaining plasma, and associated filtration device and method |
| CN105445083A (en) * | 2015-12-21 | 2016-03-30 | 丹娜（天津）生物科技有限公司 | Centrifugation-free whole-blood processing module |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108152177A (en) * | 2017-12-11 | 2018-06-12 | 三诺生物传感股份有限公司 | A kind of method of quick detection hematocrit |
| CN108152177B (en) * | 2017-12-11 | 2020-11-24 | 三诺生物传感股份有限公司 | Method for rapidly detecting hematocrit |
| WO2020220157A1 (en) * | 2019-04-27 | 2020-11-05 | 南京岚煜生物科技有限公司 | Whole blood filtering method and filter membrane structure for whole blood filtering |
| CN116731962A (en) * | 2023-08-14 | 2023-09-12 | 天津中新科炬生物制药股份有限公司 | Kit and method for separating red blood cells from whole blood |
| CN116731962B (en) * | 2023-08-14 | 2023-11-03 | 天津中新科炬生物制药股份有限公司 | Kit and method for separating red blood cells from whole blood |
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