CN106011045A - Method for preparing Sb-polluted site efficient ecological restoration flora by utilizing biogas residues - Google Patents
Method for preparing Sb-polluted site efficient ecological restoration flora by utilizing biogas residues Download PDFInfo
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- CN106011045A CN106011045A CN201610370472.5A CN201610370472A CN106011045A CN 106011045 A CN106011045 A CN 106011045A CN 201610370472 A CN201610370472 A CN 201610370472A CN 106011045 A CN106011045 A CN 106011045A
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- Prior art keywords
- sulfate
- flora
- stibate
- biogas residue
- solution
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- 238000000034 method Methods 0.000 title claims abstract description 17
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims abstract description 43
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 25
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 claims abstract description 13
- 230000001988 toxicity Effects 0.000 claims abstract description 5
- 231100000419 toxicity Toxicity 0.000 claims abstract description 5
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 17
- 229910052787 antimony Inorganic materials 0.000 claims description 10
- WATWJIUSRGPENY-UHFFFAOYSA-N antimony atom Chemical compound [Sb] WATWJIUSRGPENY-UHFFFAOYSA-N 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 230000008439 repair process Effects 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 5
- 238000005842 biochemical reaction Methods 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 3
- 239000002351 wastewater Substances 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims 1
- 229920002472 Starch Polymers 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 235000003891 ferrous sulphate Nutrition 0.000 claims 1
- 239000011790 ferrous sulphate Substances 0.000 claims 1
- 239000010794 food waste Substances 0.000 claims 1
- 229910000358 iron sulfate Inorganic materials 0.000 claims 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims 1
- 239000010815 organic waste Substances 0.000 claims 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims 1
- 229910052939 potassium sulfate Inorganic materials 0.000 claims 1
- 235000011151 potassium sulphates Nutrition 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 229910052938 sodium sulfate Inorganic materials 0.000 claims 1
- 235000011152 sodium sulphate Nutrition 0.000 claims 1
- 239000002910 solid waste Substances 0.000 claims 1
- 235000019698 starch Nutrition 0.000 claims 1
- 239000008107 starch Substances 0.000 claims 1
- 150000003568 thioethers Chemical class 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 3
- 239000002207 metabolite Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 238000011953 bioanalysis Methods 0.000 description 1
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000005987 sulfurization reaction Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 125000000101 thioether group Chemical group 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Soil Sciences (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a method for preparing Sb (V) polluted site high-efficiency ecological restoration flora by utilizing biogas residues, which is characterized in that a carbon source and sulfate are added into the biogas residues, so that sulfate reduction flora in the biogas residues can grow rapidly, and sulfides are generated at the same time; then, antimonate is added to consume sulfide for inhibiting the growth of sulfate reducing bacteria, the existence of the sulfide also greatly reduces the toxicity of the antimonate to the sulfate reducing bacteria, and simultaneously, the Sb resistance of the cultured sulfate reducing bacteria is improved.
Description
Technical field
The present invention provides a kind of and utilizes biogas residue to prepare the method that Sb contaminated site efficient ecological repairs flora, by adding carbon source and sulfate in biogas residue, makes sulfate reduction flora in biogas residue mushroom out, produces sulfide simultaneously；It is subsequently added stibate, consumes the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the stibate toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Sb performance improve simultaneously, belong to field of environment protection.
Background technology
Owing to mining, Metallurgical Waste Water arbitrarily discharge, cause a large amount of soil and polluted by Sb.The method processing mercurous soil at present is the most defective.High-temperature melting method cost is high, and power consumption is big.Bioanalysis is long for reaction time, and easily causes the secondary pollution of adventive microbial inoculum.Solidification method effect is unstable, and soil is easily caused secondary pollution by firming agent.The patent No. 201510056406.6 describes a kind of curing containing heavy metal soil, but the method using substantial amounts of material, relatively costly, on the other hand solidification effect is unstable, the most easily there is carbonation in the calcium oxide as added so that alkaline-curing effect reduces.
Summary of the invention
For the deficiencies in the prior art, the present invention provides one to utilize biogas residue to prepare the method that Sb (V) contaminated site efficient ecological repairs flora, by adding carbon source and sulfate in biogas residue, make sulfate reduction flora in biogas residue mushroom out, produce sulfide simultaneously；It is subsequently added stibate, consumes the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the stibate toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Sb performance improve simultaneously.
Its operating procedure is as follows:
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 0.1-20%, solid content 0.5-25%, organic carbon content 0.5-20%, the mass ratio of organic carbon/sulfate (counting with SO4) is (1-50): 1, following reaction 0.1-5 days；
(2) in step (1) reacted solution, a certain amount of stibate, the mol ratio (1-50) of control sulfate/stibate: 1, the mass ratio (10-100) of organic carbon/antimony: 1, following reaction 0-5 days are added；
(3) repeat to add stibate 0-4 time in solution, the most all control the mol ratio (1-50) of sulfate/stibate: 1, the mass ratio (10-100) of organic carbon/antimony: 1, in 0-5 days response time, obtain efficient ecological reparation flora subsequently.
Comparing traditional containing antimony agent, soil treatment method, this method has a following advantage:
1. the sulfate reduction flora cultivated itself is indigenous flora, and natural tolerance is strong, easily multiplies on a large scale in place, while effectively going back ortho-antimonate, does not results in ecological problem；
2. cultivating acclimation period significantly to reduce, can be trained the soonest in 1 day, economy is greatly improved；
3. while cultivating sulfate reducting bacteria, create metabolite sulfide, significantly alleviate the stibate added the subsequently toxicity to sulfate reducting bacteria, simultaneously because sulfate reducting bacteria is just had about suppression by metabolite sulfide itself, the most this method, while cultivating sulfate reducting bacteria rapidly, enhances its resistance to antimony characteristic；
4.Sb and sulfate reducting bacteria metabolite sulfide form sulfuration Sb precipitation so that in soil, Sb is more stable.
It is embodied as example as follows:
Embodiment 1:
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 0.5%, solid content 10%, organic carbon content 12%, the mass ratio of organic carbon/sulfate is 5:1, following reaction 1 day；
(2) in step (1) reacted solution, a certain amount of stibate, the mol ratio (2-3) of control sulfate/stibate: 1, the mass ratio (10-30) of organic carbon/antimony: 1, following reaction 5 days are added；
(3) repeat in solution, to add stibate 2 times, the most all control the mol ratio (2-3) of sulfate/stibate: 1, the mass ratio (10-30) of organic carbon/antimony: 1, in 5 days response time, obtain efficient ecological subsequently and repair flora.
Embodiment 2:
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 8-10%, solid content 10-15%, organic carbon content 10-15%, the mass ratio of organic carbon/sulfate is 20:1, following reaction 0.1-1 days；
(2) in step (1) reacted solution, a certain amount of stibate, the mol ratio (1-5) of control sulfate/stibate: 1, the mass ratio (30-80) of organic carbon/antimony: 1, following reaction 0-1 days are added；
(3) repeat in solution, to add stibate 1 time, the mol ratio (1-5) of control sulfate/stibate: 1, the mass ratio (30-80) of organic carbon/antimony: 1,1 day response time, obtain efficient ecological subsequently and repair flora.
Above example is only in order to illustrate technical scheme, rather than is limited；Although the present invention being described in detail with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or wherein portion of techniques feature is carried out equivalent；And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (4)
1. utilize biogas residue to prepare the method that Sb contaminated site efficient ecological repairs flora, it is characterized in that, by adding carbon source and sulfate in biogas residue, make sulfate reduction flora in biogas residue mushroom out, produce sulfide simultaneously；Being subsequently added stibate, consume the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the stibate toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Sb performance improve simultaneously, it comprises the steps of
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 0.1-20%, solid content 0.5-25%, organic carbon content 0.5-20%, the mass ratio of organic carbon/sulfate (counting with SO4) is (1-50): 1, following reaction 0.1-5 days；
(2) in step (1) reacted solution, a certain amount of stibate, the mol ratio (1-50) of control sulfate/stibate: 1, the mass ratio (10-100) of organic carbon/antimony: 1, following reaction 0-5 days are added；
(3) repeat to add stibate 0-4 time in solution, the most all control the mol ratio (1-50) of sulfate/stibate: 1, the mass ratio (10-100) of organic carbon/antimony: 1, in 0-5 days response time, obtain efficient ecological reparation flora subsequently.
A kind of utilize biogas residue prepare Sb contaminated site efficient ecological repair flora method, it is characterized in that, described biogas residue be agricultural, grocery trade debirs carry out the solid residue in dry type, half dry type and wet type anaerobic fermentation process.
A kind of method utilizing biogas residue to prepare Sb contaminated site efficient ecological reparation flora, it is characterized in that, described carbon source solution can be containing organic solution such as ethanol, saccharide, protein, starch, can also be for the solution containing the industry organic solid waste such as organic waste, changing food waste, it is also possible to for industrial and municipal organic wastewater.
A kind of method utilizing biogas residue to prepare Sb contaminated site efficient ecological reparation flora, is characterized in that, described containing sulfate is the salt of the containing sulfate radicals such as sodium sulfate, potassium sulfate, ferrous sulfate, iron sulfate；Described stibate is the salt containing stibate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610370472.5A CN106011045A (en) | 2016-05-30 | 2016-05-30 | Method for preparing Sb-polluted site efficient ecological restoration flora by utilizing biogas residues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610370472.5A CN106011045A (en) | 2016-05-30 | 2016-05-30 | Method for preparing Sb-polluted site efficient ecological restoration flora by utilizing biogas residues |
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| Publication Number | Publication Date |
|---|---|
| CN106011045A true CN106011045A (en) | 2016-10-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610370472.5A Pending CN106011045A (en) | 2016-05-30 | 2016-05-30 | Method for preparing Sb-polluted site efficient ecological restoration flora by utilizing biogas residues |
Country Status (1)
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| CN (1) | CN106011045A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1332701A (en) * | 1998-12-29 | 2002-01-23 | 帕克比奥系统公司 | Process for treatment of waste water containing heavy metals |
| CN101195859A (en) * | 2006-12-05 | 2008-06-11 | 中国科学院过程工程研究所 | Technique for processing low concentration heavy metal sulfate liquor with microorganism method |
| CN104450552A (en) * | 2014-08-17 | 2015-03-25 | 西北大学 | Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil |
-
2016
- 2016-05-30 CN CN201610370472.5A patent/CN106011045A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1332701A (en) * | 1998-12-29 | 2002-01-23 | 帕克比奥系统公司 | Process for treatment of waste water containing heavy metals |
| CN101195859A (en) * | 2006-12-05 | 2008-06-11 | 中国科学院过程工程研究所 | Technique for processing low concentration heavy metal sulfate liquor with microorganism method |
| CN104450552A (en) * | 2014-08-17 | 2015-03-25 | 西北大学 | Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil |
Non-Patent Citations (2)
| Title |
|---|
| 欧阳小雪等: "用硫酸盐还原菌去除废水中锑的实验研究", 《地球与环境》 * |
| 马春花等: "硫酸盐还原菌处理含重金属废水的实验研究", 《科技创新导报》 * |
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| C06 | Publication | ||
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| C10 | Entry into substantive examination | ||
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Application publication date: 20161012 |
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| RJ01 | Rejection of invention patent application after publication |